oligonucleotide primers Search Results


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  • 99
    Integrated DNA Technologies primer design dna oligonucleotide primers
    Primer Design Dna Oligonucleotide Primers, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher random primers dna labeling kit
    Differential display and Northern blot analysis of R5020 and epidermal growth factor (EGF) regulation of desmoplakin I (DPI) and II (DPII) in T47D cells. (a) Autoradiography of a representative differential display RT-PCR acrylamide gel. 33 P-labeled <t>DNA</t> fragments generated by RT-PCR from RNAs of control T47D cells (lane 1), 10 nmol/l R5020 treatment (lane 2), 10 nmol/l EGF treatment (lane 3), 10 nmol/l R5020 plus 10 nmol/l EGF treatment (lane 4). Each lane contains 5 μL PCR mixture. (A) 1:20 dilution of RT-PCR <t>cDNA.</t> (B) 1:40 dilution of RT-PCR cDNA. The arrow indicates desmoplakin (DP). Molecular weight standards of 0.65, 0.42, and 0.41 kb are indicated in lane 5. (b) Photograph of a representative Northern blot analysis of DPI and DPII, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA expression in T47D cells. Lane 1: control T47D cells were left untreated for 48 hours. Matched sister colonies of T47D cells received 10nmol/l R5020 (lane 2), 10 nmol/l EGF (lane 3), or 10 nmol/l R5020 plus 10 nmol/l EGF for 48 hours (lane 4). Ten micrograms of total RNA was subjected to Northern blot analysis as described in the Methods section. (c) The Northern blot films were scanned. The graphs indicate the fold difference of desmoplakin expression compared vehicle (set at a value of 1) following measurement of the band intensities. In each independent experiment, the desmoplakin band for each treatment condition was normalized to the GAPDH signal. The experiment was repeated three times and the mean values from these experiments ± SEM are reported. Statistical significance was determined using t-test. * P
    Random Primers Dna Labeling Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 228 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Thermo Fisher dna oligonucleotide primers
    Differential display and Northern blot analysis of R5020 and epidermal growth factor (EGF) regulation of desmoplakin I (DPI) and II (DPII) in T47D cells. (a) Autoradiography of a representative differential display RT-PCR acrylamide gel. 33 P-labeled <t>DNA</t> fragments generated by RT-PCR from RNAs of control T47D cells (lane 1), 10 nmol/l R5020 treatment (lane 2), 10 nmol/l EGF treatment (lane 3), 10 nmol/l R5020 plus 10 nmol/l EGF treatment (lane 4). Each lane contains 5 μL PCR mixture. (A) 1:20 dilution of RT-PCR <t>cDNA.</t> (B) 1:40 dilution of RT-PCR cDNA. The arrow indicates desmoplakin (DP). Molecular weight standards of 0.65, 0.42, and 0.41 kb are indicated in lane 5. (b) Photograph of a representative Northern blot analysis of DPI and DPII, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA expression in T47D cells. Lane 1: control T47D cells were left untreated for 48 hours. Matched sister colonies of T47D cells received 10nmol/l R5020 (lane 2), 10 nmol/l EGF (lane 3), or 10 nmol/l R5020 plus 10 nmol/l EGF for 48 hours (lane 4). Ten micrograms of total RNA was subjected to Northern blot analysis as described in the Methods section. (c) The Northern blot films were scanned. The graphs indicate the fold difference of desmoplakin expression compared vehicle (set at a value of 1) following measurement of the band intensities. In each independent experiment, the desmoplakin band for each treatment condition was normalized to the GAPDH signal. The experiment was repeated three times and the mean values from these experiments ± SEM are reported. Statistical significance was determined using t-test. * P
    Dna Oligonucleotide Primers, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 91 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    biomers.net dna oligonucleotide primers
    Differential display and Northern blot analysis of R5020 and epidermal growth factor (EGF) regulation of desmoplakin I (DPI) and II (DPII) in T47D cells. (a) Autoradiography of a representative differential display RT-PCR acrylamide gel. 33 P-labeled <t>DNA</t> fragments generated by RT-PCR from RNAs of control T47D cells (lane 1), 10 nmol/l R5020 treatment (lane 2), 10 nmol/l EGF treatment (lane 3), 10 nmol/l R5020 plus 10 nmol/l EGF treatment (lane 4). Each lane contains 5 μL PCR mixture. (A) 1:20 dilution of RT-PCR <t>cDNA.</t> (B) 1:40 dilution of RT-PCR cDNA. The arrow indicates desmoplakin (DP). Molecular weight standards of 0.65, 0.42, and 0.41 kb are indicated in lane 5. (b) Photograph of a representative Northern blot analysis of DPI and DPII, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA expression in T47D cells. Lane 1: control T47D cells were left untreated for 48 hours. Matched sister colonies of T47D cells received 10nmol/l R5020 (lane 2), 10 nmol/l EGF (lane 3), or 10 nmol/l R5020 plus 10 nmol/l EGF for 48 hours (lane 4). Ten micrograms of total RNA was subjected to Northern blot analysis as described in the Methods section. (c) The Northern blot films were scanned. The graphs indicate the fold difference of desmoplakin expression compared vehicle (set at a value of 1) following measurement of the band intensities. In each independent experiment, the desmoplakin band for each treatment condition was normalized to the GAPDH signal. The experiment was repeated three times and the mean values from these experiments ± SEM are reported. Statistical significance was determined using t-test. * P
    Dna Oligonucleotide Primers, supplied by biomers.net, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Roche dna oligonucleotide primers
    Differential display and Northern blot analysis of R5020 and epidermal growth factor (EGF) regulation of desmoplakin I (DPI) and II (DPII) in T47D cells. (a) Autoradiography of a representative differential display RT-PCR acrylamide gel. 33 P-labeled <t>DNA</t> fragments generated by RT-PCR from RNAs of control T47D cells (lane 1), 10 nmol/l R5020 treatment (lane 2), 10 nmol/l EGF treatment (lane 3), 10 nmol/l R5020 plus 10 nmol/l EGF treatment (lane 4). Each lane contains 5 μL PCR mixture. (A) 1:20 dilution of RT-PCR <t>cDNA.</t> (B) 1:40 dilution of RT-PCR cDNA. The arrow indicates desmoplakin (DP). Molecular weight standards of 0.65, 0.42, and 0.41 kb are indicated in lane 5. (b) Photograph of a representative Northern blot analysis of DPI and DPII, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA expression in T47D cells. Lane 1: control T47D cells were left untreated for 48 hours. Matched sister colonies of T47D cells received 10nmol/l R5020 (lane 2), 10 nmol/l EGF (lane 3), or 10 nmol/l R5020 plus 10 nmol/l EGF for 48 hours (lane 4). Ten micrograms of total RNA was subjected to Northern blot analysis as described in the Methods section. (c) The Northern blot films were scanned. The graphs indicate the fold difference of desmoplakin expression compared vehicle (set at a value of 1) following measurement of the band intensities. In each independent experiment, the desmoplakin band for each treatment condition was normalized to the GAPDH signal. The experiment was repeated three times and the mean values from these experiments ± SEM are reported. Statistical significance was determined using t-test. * P
    Dna Oligonucleotide Primers, supplied by Roche, used in various techniques. Bioz Stars score: 89/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Sigma-Genosys dna oligonucleotide primers
    Differential display and Northern blot analysis of R5020 and epidermal growth factor (EGF) regulation of desmoplakin I (DPI) and II (DPII) in T47D cells. (a) Autoradiography of a representative differential display RT-PCR acrylamide gel. 33 P-labeled <t>DNA</t> fragments generated by RT-PCR from RNAs of control T47D cells (lane 1), 10 nmol/l R5020 treatment (lane 2), 10 nmol/l EGF treatment (lane 3), 10 nmol/l R5020 plus 10 nmol/l EGF treatment (lane 4). Each lane contains 5 μL PCR mixture. (A) 1:20 dilution of RT-PCR <t>cDNA.</t> (B) 1:40 dilution of RT-PCR cDNA. The arrow indicates desmoplakin (DP). Molecular weight standards of 0.65, 0.42, and 0.41 kb are indicated in lane 5. (b) Photograph of a representative Northern blot analysis of DPI and DPII, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA expression in T47D cells. Lane 1: control T47D cells were left untreated for 48 hours. Matched sister colonies of T47D cells received 10nmol/l R5020 (lane 2), 10 nmol/l EGF (lane 3), or 10 nmol/l R5020 plus 10 nmol/l EGF for 48 hours (lane 4). Ten micrograms of total RNA was subjected to Northern blot analysis as described in the Methods section. (c) The Northern blot films were scanned. The graphs indicate the fold difference of desmoplakin expression compared vehicle (set at a value of 1) following measurement of the band intensities. In each independent experiment, the desmoplakin band for each treatment condition was normalized to the GAPDH signal. The experiment was repeated three times and the mean values from these experiments ± SEM are reported. Statistical significance was determined using t-test. * P
    Dna Oligonucleotide Primers, supplied by Sigma-Genosys, used in various techniques. Bioz Stars score: 89/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Sigma-Genosys dna sequencing oligonucleotide primers
    Differential display and Northern blot analysis of R5020 and epidermal growth factor (EGF) regulation of desmoplakin I (DPI) and II (DPII) in T47D cells. (a) Autoradiography of a representative differential display RT-PCR acrylamide gel. 33 P-labeled <t>DNA</t> fragments generated by RT-PCR from RNAs of control T47D cells (lane 1), 10 nmol/l R5020 treatment (lane 2), 10 nmol/l EGF treatment (lane 3), 10 nmol/l R5020 plus 10 nmol/l EGF treatment (lane 4). Each lane contains 5 μL PCR mixture. (A) 1:20 dilution of RT-PCR <t>cDNA.</t> (B) 1:40 dilution of RT-PCR cDNA. The arrow indicates desmoplakin (DP). Molecular weight standards of 0.65, 0.42, and 0.41 kb are indicated in lane 5. (b) Photograph of a representative Northern blot analysis of DPI and DPII, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA expression in T47D cells. Lane 1: control T47D cells were left untreated for 48 hours. Matched sister colonies of T47D cells received 10nmol/l R5020 (lane 2), 10 nmol/l EGF (lane 3), or 10 nmol/l R5020 plus 10 nmol/l EGF for 48 hours (lane 4). Ten micrograms of total RNA was subjected to Northern blot analysis as described in the Methods section. (c) The Northern blot films were scanned. The graphs indicate the fold difference of desmoplakin expression compared vehicle (set at a value of 1) following measurement of the band intensities. In each independent experiment, the desmoplakin band for each treatment condition was normalized to the GAPDH signal. The experiment was repeated three times and the mean values from these experiments ± SEM are reported. Statistical significance was determined using t-test. * P
    Dna Sequencing Oligonucleotide Primers, supplied by Sigma-Genosys, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    GenScript oligonucleotide dna primers
    Differential display and Northern blot analysis of R5020 and epidermal growth factor (EGF) regulation of desmoplakin I (DPI) and II (DPII) in T47D cells. (a) Autoradiography of a representative differential display RT-PCR acrylamide gel. 33 P-labeled <t>DNA</t> fragments generated by RT-PCR from RNAs of control T47D cells (lane 1), 10 nmol/l R5020 treatment (lane 2), 10 nmol/l EGF treatment (lane 3), 10 nmol/l R5020 plus 10 nmol/l EGF treatment (lane 4). Each lane contains 5 μL PCR mixture. (A) 1:20 dilution of RT-PCR <t>cDNA.</t> (B) 1:40 dilution of RT-PCR cDNA. The arrow indicates desmoplakin (DP). Molecular weight standards of 0.65, 0.42, and 0.41 kb are indicated in lane 5. (b) Photograph of a representative Northern blot analysis of DPI and DPII, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA expression in T47D cells. Lane 1: control T47D cells were left untreated for 48 hours. Matched sister colonies of T47D cells received 10nmol/l R5020 (lane 2), 10 nmol/l EGF (lane 3), or 10 nmol/l R5020 plus 10 nmol/l EGF for 48 hours (lane 4). Ten micrograms of total RNA was subjected to Northern blot analysis as described in the Methods section. (c) The Northern blot films were scanned. The graphs indicate the fold difference of desmoplakin expression compared vehicle (set at a value of 1) following measurement of the band intensities. In each independent experiment, the desmoplakin band for each treatment condition was normalized to the GAPDH signal. The experiment was repeated three times and the mean values from these experiments ± SEM are reported. Statistical significance was determined using t-test. * P
    Oligonucleotide Dna Primers, supplied by GenScript, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore complementary dna oligonucleotide primers
    Differential display and Northern blot analysis of R5020 and epidermal growth factor (EGF) regulation of desmoplakin I (DPI) and II (DPII) in T47D cells. (a) Autoradiography of a representative differential display RT-PCR acrylamide gel. 33 P-labeled <t>DNA</t> fragments generated by RT-PCR from RNAs of control T47D cells (lane 1), 10 nmol/l R5020 treatment (lane 2), 10 nmol/l EGF treatment (lane 3), 10 nmol/l R5020 plus 10 nmol/l EGF treatment (lane 4). Each lane contains 5 μL PCR mixture. (A) 1:20 dilution of RT-PCR <t>cDNA.</t> (B) 1:40 dilution of RT-PCR cDNA. The arrow indicates desmoplakin (DP). Molecular weight standards of 0.65, 0.42, and 0.41 kb are indicated in lane 5. (b) Photograph of a representative Northern blot analysis of DPI and DPII, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA expression in T47D cells. Lane 1: control T47D cells were left untreated for 48 hours. Matched sister colonies of T47D cells received 10nmol/l R5020 (lane 2), 10 nmol/l EGF (lane 3), or 10 nmol/l R5020 plus 10 nmol/l EGF for 48 hours (lane 4). Ten micrograms of total RNA was subjected to Northern blot analysis as described in the Methods section. (c) The Northern blot films were scanned. The graphs indicate the fold difference of desmoplakin expression compared vehicle (set at a value of 1) following measurement of the band intensities. In each independent experiment, the desmoplakin band for each treatment condition was normalized to the GAPDH signal. The experiment was repeated three times and the mean values from these experiments ± SEM are reported. Statistical significance was determined using t-test. * P
    Complementary Dna Oligonucleotide Primers, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa oligonucleotide dna primers
    Differential display and Northern blot analysis of R5020 and epidermal growth factor (EGF) regulation of desmoplakin I (DPI) and II (DPII) in T47D cells. (a) Autoradiography of a representative differential display RT-PCR acrylamide gel. 33 P-labeled <t>DNA</t> fragments generated by RT-PCR from RNAs of control T47D cells (lane 1), 10 nmol/l R5020 treatment (lane 2), 10 nmol/l EGF treatment (lane 3), 10 nmol/l R5020 plus 10 nmol/l EGF treatment (lane 4). Each lane contains 5 μL PCR mixture. (A) 1:20 dilution of RT-PCR <t>cDNA.</t> (B) 1:40 dilution of RT-PCR cDNA. The arrow indicates desmoplakin (DP). Molecular weight standards of 0.65, 0.42, and 0.41 kb are indicated in lane 5. (b) Photograph of a representative Northern blot analysis of DPI and DPII, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA expression in T47D cells. Lane 1: control T47D cells were left untreated for 48 hours. Matched sister colonies of T47D cells received 10nmol/l R5020 (lane 2), 10 nmol/l EGF (lane 3), or 10 nmol/l R5020 plus 10 nmol/l EGF for 48 hours (lane 4). Ten micrograms of total RNA was subjected to Northern blot analysis as described in the Methods section. (c) The Northern blot films were scanned. The graphs indicate the fold difference of desmoplakin expression compared vehicle (set at a value of 1) following measurement of the band intensities. In each independent experiment, the desmoplakin band for each treatment condition was normalized to the GAPDH signal. The experiment was repeated three times and the mean values from these experiments ± SEM are reported. Statistical significance was determined using t-test. * P
    Oligonucleotide Dna Primers, supplied by TaKaRa, used in various techniques. Bioz Stars score: 88/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare oligonucleotide dna primers
    Differential display and Northern blot analysis of R5020 and epidermal growth factor (EGF) regulation of desmoplakin I (DPI) and II (DPII) in T47D cells. (a) Autoradiography of a representative differential display RT-PCR acrylamide gel. 33 P-labeled <t>DNA</t> fragments generated by RT-PCR from RNAs of control T47D cells (lane 1), 10 nmol/l R5020 treatment (lane 2), 10 nmol/l EGF treatment (lane 3), 10 nmol/l R5020 plus 10 nmol/l EGF treatment (lane 4). Each lane contains 5 μL PCR mixture. (A) 1:20 dilution of RT-PCR <t>cDNA.</t> (B) 1:40 dilution of RT-PCR cDNA. The arrow indicates desmoplakin (DP). Molecular weight standards of 0.65, 0.42, and 0.41 kb are indicated in lane 5. (b) Photograph of a representative Northern blot analysis of DPI and DPII, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA expression in T47D cells. Lane 1: control T47D cells were left untreated for 48 hours. Matched sister colonies of T47D cells received 10nmol/l R5020 (lane 2), 10 nmol/l EGF (lane 3), or 10 nmol/l R5020 plus 10 nmol/l EGF for 48 hours (lane 4). Ten micrograms of total RNA was subjected to Northern blot analysis as described in the Methods section. (c) The Northern blot films were scanned. The graphs indicate the fold difference of desmoplakin expression compared vehicle (set at a value of 1) following measurement of the band intensities. In each independent experiment, the desmoplakin band for each treatment condition was normalized to the GAPDH signal. The experiment was repeated three times and the mean values from these experiments ± SEM are reported. Statistical significance was determined using t-test. * P
    Oligonucleotide Dna Primers, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 88/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TIB MOLBIOL dna oligonucleotide primers
    Differential display and Northern blot analysis of R5020 and epidermal growth factor (EGF) regulation of desmoplakin I (DPI) and II (DPII) in T47D cells. (a) Autoradiography of a representative differential display RT-PCR acrylamide gel. 33 P-labeled <t>DNA</t> fragments generated by RT-PCR from RNAs of control T47D cells (lane 1), 10 nmol/l R5020 treatment (lane 2), 10 nmol/l EGF treatment (lane 3), 10 nmol/l R5020 plus 10 nmol/l EGF treatment (lane 4). Each lane contains 5 μL PCR mixture. (A) 1:20 dilution of RT-PCR <t>cDNA.</t> (B) 1:40 dilution of RT-PCR cDNA. The arrow indicates desmoplakin (DP). Molecular weight standards of 0.65, 0.42, and 0.41 kb are indicated in lane 5. (b) Photograph of a representative Northern blot analysis of DPI and DPII, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA expression in T47D cells. Lane 1: control T47D cells were left untreated for 48 hours. Matched sister colonies of T47D cells received 10nmol/l R5020 (lane 2), 10 nmol/l EGF (lane 3), or 10 nmol/l R5020 plus 10 nmol/l EGF for 48 hours (lane 4). Ten micrograms of total RNA was subjected to Northern blot analysis as described in the Methods section. (c) The Northern blot films were scanned. The graphs indicate the fold difference of desmoplakin expression compared vehicle (set at a value of 1) following measurement of the band intensities. In each independent experiment, the desmoplakin band for each treatment condition was normalized to the GAPDH signal. The experiment was repeated three times and the mean values from these experiments ± SEM are reported. Statistical significance was determined using t-test. * P
    Dna Oligonucleotide Primers, supplied by TIB MOLBIOL, used in various techniques. Bioz Stars score: 89/100, based on 120 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Sangon Biotech dna oligonucleotide primers
    Differential display and Northern blot analysis of R5020 and epidermal growth factor (EGF) regulation of desmoplakin I (DPI) and II (DPII) in T47D cells. (a) Autoradiography of a representative differential display RT-PCR acrylamide gel. 33 P-labeled <t>DNA</t> fragments generated by RT-PCR from RNAs of control T47D cells (lane 1), 10 nmol/l R5020 treatment (lane 2), 10 nmol/l EGF treatment (lane 3), 10 nmol/l R5020 plus 10 nmol/l EGF treatment (lane 4). Each lane contains 5 μL PCR mixture. (A) 1:20 dilution of RT-PCR <t>cDNA.</t> (B) 1:40 dilution of RT-PCR cDNA. The arrow indicates desmoplakin (DP). Molecular weight standards of 0.65, 0.42, and 0.41 kb are indicated in lane 5. (b) Photograph of a representative Northern blot analysis of DPI and DPII, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA expression in T47D cells. Lane 1: control T47D cells were left untreated for 48 hours. Matched sister colonies of T47D cells received 10nmol/l R5020 (lane 2), 10 nmol/l EGF (lane 3), or 10 nmol/l R5020 plus 10 nmol/l EGF for 48 hours (lane 4). Ten micrograms of total RNA was subjected to Northern blot analysis as described in the Methods section. (c) The Northern blot films were scanned. The graphs indicate the fold difference of desmoplakin expression compared vehicle (set at a value of 1) following measurement of the band intensities. In each independent experiment, the desmoplakin band for each treatment condition was normalized to the GAPDH signal. The experiment was repeated three times and the mean values from these experiments ± SEM are reported. Statistical significance was determined using t-test. * P
    Dna Oligonucleotide Primers, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Beckman Coulter dna oligonucleotide primers
    Differential display and Northern blot analysis of R5020 and epidermal growth factor (EGF) regulation of desmoplakin I (DPI) and II (DPII) in T47D cells. (a) Autoradiography of a representative differential display RT-PCR acrylamide gel. 33 P-labeled <t>DNA</t> fragments generated by RT-PCR from RNAs of control T47D cells (lane 1), 10 nmol/l R5020 treatment (lane 2), 10 nmol/l EGF treatment (lane 3), 10 nmol/l R5020 plus 10 nmol/l EGF treatment (lane 4). Each lane contains 5 μL PCR mixture. (A) 1:20 dilution of RT-PCR <t>cDNA.</t> (B) 1:40 dilution of RT-PCR cDNA. The arrow indicates desmoplakin (DP). Molecular weight standards of 0.65, 0.42, and 0.41 kb are indicated in lane 5. (b) Photograph of a representative Northern blot analysis of DPI and DPII, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA expression in T47D cells. Lane 1: control T47D cells were left untreated for 48 hours. Matched sister colonies of T47D cells received 10nmol/l R5020 (lane 2), 10 nmol/l EGF (lane 3), or 10 nmol/l R5020 plus 10 nmol/l EGF for 48 hours (lane 4). Ten micrograms of total RNA was subjected to Northern blot analysis as described in the Methods section. (c) The Northern blot films were scanned. The graphs indicate the fold difference of desmoplakin expression compared vehicle (set at a value of 1) following measurement of the band intensities. In each independent experiment, the desmoplakin band for each treatment condition was normalized to the GAPDH signal. The experiment was repeated three times and the mean values from these experiments ± SEM are reported. Statistical significance was determined using t-test. * P
    Dna Oligonucleotide Primers, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 89/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Kaneka Corp dna oligonucleotide primers
    Differential display and Northern blot analysis of R5020 and epidermal growth factor (EGF) regulation of desmoplakin I (DPI) and II (DPII) in T47D cells. (a) Autoradiography of a representative differential display RT-PCR acrylamide gel. 33 P-labeled <t>DNA</t> fragments generated by RT-PCR from RNAs of control T47D cells (lane 1), 10 nmol/l R5020 treatment (lane 2), 10 nmol/l EGF treatment (lane 3), 10 nmol/l R5020 plus 10 nmol/l EGF treatment (lane 4). Each lane contains 5 μL PCR mixture. (A) 1:20 dilution of RT-PCR <t>cDNA.</t> (B) 1:40 dilution of RT-PCR cDNA. The arrow indicates desmoplakin (DP). Molecular weight standards of 0.65, 0.42, and 0.41 kb are indicated in lane 5. (b) Photograph of a representative Northern blot analysis of DPI and DPII, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA expression in T47D cells. Lane 1: control T47D cells were left untreated for 48 hours. Matched sister colonies of T47D cells received 10nmol/l R5020 (lane 2), 10 nmol/l EGF (lane 3), or 10 nmol/l R5020 plus 10 nmol/l EGF for 48 hours (lane 4). Ten micrograms of total RNA was subjected to Northern blot analysis as described in the Methods section. (c) The Northern blot films were scanned. The graphs indicate the fold difference of desmoplakin expression compared vehicle (set at a value of 1) following measurement of the band intensities. In each independent experiment, the desmoplakin band for each treatment condition was normalized to the GAPDH signal. The experiment was repeated three times and the mean values from these experiments ± SEM are reported. Statistical significance was determined using t-test. * P
    Dna Oligonucleotide Primers, supplied by Kaneka Corp, used in various techniques. Bioz Stars score: 90/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Differential display and Northern blot analysis of R5020 and epidermal growth factor (EGF) regulation of desmoplakin I (DPI) and II (DPII) in T47D cells. (a) Autoradiography of a representative differential display RT-PCR acrylamide gel. 33 P-labeled <t>DNA</t> fragments generated by RT-PCR from RNAs of control T47D cells (lane 1), 10 nmol/l R5020 treatment (lane 2), 10 nmol/l EGF treatment (lane 3), 10 nmol/l R5020 plus 10 nmol/l EGF treatment (lane 4). Each lane contains 5 μL PCR mixture. (A) 1:20 dilution of RT-PCR <t>cDNA.</t> (B) 1:40 dilution of RT-PCR cDNA. The arrow indicates desmoplakin (DP). Molecular weight standards of 0.65, 0.42, and 0.41 kb are indicated in lane 5. (b) Photograph of a representative Northern blot analysis of DPI and DPII, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA expression in T47D cells. Lane 1: control T47D cells were left untreated for 48 hours. Matched sister colonies of T47D cells received 10nmol/l R5020 (lane 2), 10 nmol/l EGF (lane 3), or 10 nmol/l R5020 plus 10 nmol/l EGF for 48 hours (lane 4). Ten micrograms of total RNA was subjected to Northern blot analysis as described in the Methods section. (c) The Northern blot films were scanned. The graphs indicate the fold difference of desmoplakin expression compared vehicle (set at a value of 1) following measurement of the band intensities. In each independent experiment, the desmoplakin band for each treatment condition was normalized to the GAPDH signal. The experiment was repeated three times and the mean values from these experiments ± SEM are reported. Statistical significance was determined using t-test. * P
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    Differential display and Northern blot analysis of R5020 and epidermal growth factor (EGF) regulation of desmoplakin I (DPI) and II (DPII) in T47D cells. (a) Autoradiography of a representative differential display RT-PCR acrylamide gel. 33 P-labeled <t>DNA</t> fragments generated by RT-PCR from RNAs of control T47D cells (lane 1), 10 nmol/l R5020 treatment (lane 2), 10 nmol/l EGF treatment (lane 3), 10 nmol/l R5020 plus 10 nmol/l EGF treatment (lane 4). Each lane contains 5 μL PCR mixture. (A) 1:20 dilution of RT-PCR <t>cDNA.</t> (B) 1:40 dilution of RT-PCR cDNA. The arrow indicates desmoplakin (DP). Molecular weight standards of 0.65, 0.42, and 0.41 kb are indicated in lane 5. (b) Photograph of a representative Northern blot analysis of DPI and DPII, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA expression in T47D cells. Lane 1: control T47D cells were left untreated for 48 hours. Matched sister colonies of T47D cells received 10nmol/l R5020 (lane 2), 10 nmol/l EGF (lane 3), or 10 nmol/l R5020 plus 10 nmol/l EGF for 48 hours (lane 4). Ten micrograms of total RNA was subjected to Northern blot analysis as described in the Methods section. (c) The Northern blot films were scanned. The graphs indicate the fold difference of desmoplakin expression compared vehicle (set at a value of 1) following measurement of the band intensities. In each independent experiment, the desmoplakin band for each treatment condition was normalized to the GAPDH signal. The experiment was repeated three times and the mean values from these experiments ± SEM are reported. Statistical significance was determined using t-test. * P
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    Differential display and Northern blot analysis of R5020 and epidermal growth factor (EGF) regulation of desmoplakin I (DPI) and II (DPII) in T47D cells. (a) Autoradiography of a representative differential display RT-PCR acrylamide gel. 33 P-labeled <t>DNA</t> fragments generated by RT-PCR from RNAs of control T47D cells (lane 1), 10 nmol/l R5020 treatment (lane 2), 10 nmol/l EGF treatment (lane 3), 10 nmol/l R5020 plus 10 nmol/l EGF treatment (lane 4). Each lane contains 5 μL PCR mixture. (A) 1:20 dilution of RT-PCR <t>cDNA.</t> (B) 1:40 dilution of RT-PCR cDNA. The arrow indicates desmoplakin (DP). Molecular weight standards of 0.65, 0.42, and 0.41 kb are indicated in lane 5. (b) Photograph of a representative Northern blot analysis of DPI and DPII, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA expression in T47D cells. Lane 1: control T47D cells were left untreated for 48 hours. Matched sister colonies of T47D cells received 10nmol/l R5020 (lane 2), 10 nmol/l EGF (lane 3), or 10 nmol/l R5020 plus 10 nmol/l EGF for 48 hours (lane 4). Ten micrograms of total RNA was subjected to Northern blot analysis as described in the Methods section. (c) The Northern blot films were scanned. The graphs indicate the fold difference of desmoplakin expression compared vehicle (set at a value of 1) following measurement of the band intensities. In each independent experiment, the desmoplakin band for each treatment condition was normalized to the GAPDH signal. The experiment was repeated three times and the mean values from these experiments ± SEM are reported. Statistical significance was determined using t-test. * P
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    Differential display and Northern blot analysis of R5020 and epidermal growth factor (EGF) regulation of desmoplakin I (DPI) and II (DPII) in T47D cells. (a) Autoradiography of a representative differential display RT-PCR acrylamide gel. 33 P-labeled <t>DNA</t> fragments generated by RT-PCR from RNAs of control T47D cells (lane 1), 10 nmol/l R5020 treatment (lane 2), 10 nmol/l EGF treatment (lane 3), 10 nmol/l R5020 plus 10 nmol/l EGF treatment (lane 4). Each lane contains 5 μL PCR mixture. (A) 1:20 dilution of RT-PCR <t>cDNA.</t> (B) 1:40 dilution of RT-PCR cDNA. The arrow indicates desmoplakin (DP). Molecular weight standards of 0.65, 0.42, and 0.41 kb are indicated in lane 5. (b) Photograph of a representative Northern blot analysis of DPI and DPII, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA expression in T47D cells. Lane 1: control T47D cells were left untreated for 48 hours. Matched sister colonies of T47D cells received 10nmol/l R5020 (lane 2), 10 nmol/l EGF (lane 3), or 10 nmol/l R5020 plus 10 nmol/l EGF for 48 hours (lane 4). Ten micrograms of total RNA was subjected to Northern blot analysis as described in the Methods section. (c) The Northern blot films were scanned. The graphs indicate the fold difference of desmoplakin expression compared vehicle (set at a value of 1) following measurement of the band intensities. In each independent experiment, the desmoplakin band for each treatment condition was normalized to the GAPDH signal. The experiment was repeated three times and the mean values from these experiments ± SEM are reported. Statistical significance was determined using t-test. * P
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    Differential display and Northern blot analysis of R5020 and epidermal growth factor (EGF) regulation of desmoplakin I (DPI) and II (DPII) in T47D cells. (a) Autoradiography of a representative differential display RT-PCR acrylamide gel. 33 P-labeled <t>DNA</t> fragments generated by RT-PCR from RNAs of control T47D cells (lane 1), 10 nmol/l R5020 treatment (lane 2), 10 nmol/l EGF treatment (lane 3), 10 nmol/l R5020 plus 10 nmol/l EGF treatment (lane 4). Each lane contains 5 μL PCR mixture. (A) 1:20 dilution of RT-PCR <t>cDNA.</t> (B) 1:40 dilution of RT-PCR cDNA. The arrow indicates desmoplakin (DP). Molecular weight standards of 0.65, 0.42, and 0.41 kb are indicated in lane 5. (b) Photograph of a representative Northern blot analysis of DPI and DPII, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA expression in T47D cells. Lane 1: control T47D cells were left untreated for 48 hours. Matched sister colonies of T47D cells received 10nmol/l R5020 (lane 2), 10 nmol/l EGF (lane 3), or 10 nmol/l R5020 plus 10 nmol/l EGF for 48 hours (lane 4). Ten micrograms of total RNA was subjected to Northern blot analysis as described in the Methods section. (c) The Northern blot films were scanned. The graphs indicate the fold difference of desmoplakin expression compared vehicle (set at a value of 1) following measurement of the band intensities. In each independent experiment, the desmoplakin band for each treatment condition was normalized to the GAPDH signal. The experiment was repeated three times and the mean values from these experiments ± SEM are reported. Statistical significance was determined using t-test. * P
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    Differential display and Northern blot analysis of R5020 and epidermal growth factor (EGF) regulation of desmoplakin I (DPI) and II (DPII) in T47D cells. (a) Autoradiography of a representative differential display RT-PCR acrylamide gel. 33 P-labeled <t>DNA</t> fragments generated by RT-PCR from RNAs of control T47D cells (lane 1), 10 nmol/l R5020 treatment (lane 2), 10 nmol/l EGF treatment (lane 3), 10 nmol/l R5020 plus 10 nmol/l EGF treatment (lane 4). Each lane contains 5 μL PCR mixture. (A) 1:20 dilution of RT-PCR <t>cDNA.</t> (B) 1:40 dilution of RT-PCR cDNA. The arrow indicates desmoplakin (DP). Molecular weight standards of 0.65, 0.42, and 0.41 kb are indicated in lane 5. (b) Photograph of a representative Northern blot analysis of DPI and DPII, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA expression in T47D cells. Lane 1: control T47D cells were left untreated for 48 hours. Matched sister colonies of T47D cells received 10nmol/l R5020 (lane 2), 10 nmol/l EGF (lane 3), or 10 nmol/l R5020 plus 10 nmol/l EGF for 48 hours (lane 4). Ten micrograms of total RNA was subjected to Northern blot analysis as described in the Methods section. (c) The Northern blot films were scanned. The graphs indicate the fold difference of desmoplakin expression compared vehicle (set at a value of 1) following measurement of the band intensities. In each independent experiment, the desmoplakin band for each treatment condition was normalized to the GAPDH signal. The experiment was repeated three times and the mean values from these experiments ± SEM are reported. Statistical significance was determined using t-test. * P
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    Differential display and Northern blot analysis of R5020 and epidermal growth factor (EGF) regulation of desmoplakin I (DPI) and II (DPII) in T47D cells. (a) Autoradiography of a representative differential display RT-PCR acrylamide gel. 33 P-labeled <t>DNA</t> fragments generated by RT-PCR from RNAs of control T47D cells (lane 1), 10 nmol/l R5020 treatment (lane 2), 10 nmol/l EGF treatment (lane 3), 10 nmol/l R5020 plus 10 nmol/l EGF treatment (lane 4). Each lane contains 5 μL PCR mixture. (A) 1:20 dilution of RT-PCR <t>cDNA.</t> (B) 1:40 dilution of RT-PCR cDNA. The arrow indicates desmoplakin (DP). Molecular weight standards of 0.65, 0.42, and 0.41 kb are indicated in lane 5. (b) Photograph of a representative Northern blot analysis of DPI and DPII, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA expression in T47D cells. Lane 1: control T47D cells were left untreated for 48 hours. Matched sister colonies of T47D cells received 10nmol/l R5020 (lane 2), 10 nmol/l EGF (lane 3), or 10 nmol/l R5020 plus 10 nmol/l EGF for 48 hours (lane 4). Ten micrograms of total RNA was subjected to Northern blot analysis as described in the Methods section. (c) The Northern blot films were scanned. The graphs indicate the fold difference of desmoplakin expression compared vehicle (set at a value of 1) following measurement of the band intensities. In each independent experiment, the desmoplakin band for each treatment condition was normalized to the GAPDH signal. The experiment was repeated three times and the mean values from these experiments ± SEM are reported. Statistical significance was determined using t-test. * P
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    Differential display and Northern blot analysis of R5020 and epidermal growth factor (EGF) regulation of desmoplakin I (DPI) and II (DPII) in T47D cells. (a) Autoradiography of a representative differential display RT-PCR acrylamide gel. 33 P-labeled <t>DNA</t> fragments generated by RT-PCR from RNAs of control T47D cells (lane 1), 10 nmol/l R5020 treatment (lane 2), 10 nmol/l EGF treatment (lane 3), 10 nmol/l R5020 plus 10 nmol/l EGF treatment (lane 4). Each lane contains 5 μL PCR mixture. (A) 1:20 dilution of RT-PCR <t>cDNA.</t> (B) 1:40 dilution of RT-PCR cDNA. The arrow indicates desmoplakin (DP). Molecular weight standards of 0.65, 0.42, and 0.41 kb are indicated in lane 5. (b) Photograph of a representative Northern blot analysis of DPI and DPII, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA expression in T47D cells. Lane 1: control T47D cells were left untreated for 48 hours. Matched sister colonies of T47D cells received 10nmol/l R5020 (lane 2), 10 nmol/l EGF (lane 3), or 10 nmol/l R5020 plus 10 nmol/l EGF for 48 hours (lane 4). Ten micrograms of total RNA was subjected to Northern blot analysis as described in the Methods section. (c) The Northern blot films were scanned. The graphs indicate the fold difference of desmoplakin expression compared vehicle (set at a value of 1) following measurement of the band intensities. In each independent experiment, the desmoplakin band for each treatment condition was normalized to the GAPDH signal. The experiment was repeated three times and the mean values from these experiments ± SEM are reported. Statistical significance was determined using t-test. * P
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    Differential display and Northern blot analysis of R5020 and epidermal growth factor (EGF) regulation of desmoplakin I (DPI) and II (DPII) in T47D cells. (a) Autoradiography of a representative differential display RT-PCR acrylamide gel. 33 P-labeled <t>DNA</t> fragments generated by RT-PCR from RNAs of control T47D cells (lane 1), 10 nmol/l R5020 treatment (lane 2), 10 nmol/l EGF treatment (lane 3), 10 nmol/l R5020 plus 10 nmol/l EGF treatment (lane 4). Each lane contains 5 μL PCR mixture. (A) 1:20 dilution of RT-PCR <t>cDNA.</t> (B) 1:40 dilution of RT-PCR cDNA. The arrow indicates desmoplakin (DP). Molecular weight standards of 0.65, 0.42, and 0.41 kb are indicated in lane 5. (b) Photograph of a representative Northern blot analysis of DPI and DPII, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA expression in T47D cells. Lane 1: control T47D cells were left untreated for 48 hours. Matched sister colonies of T47D cells received 10nmol/l R5020 (lane 2), 10 nmol/l EGF (lane 3), or 10 nmol/l R5020 plus 10 nmol/l EGF for 48 hours (lane 4). Ten micrograms of total RNA was subjected to Northern blot analysis as described in the Methods section. (c) The Northern blot films were scanned. The graphs indicate the fold difference of desmoplakin expression compared vehicle (set at a value of 1) following measurement of the band intensities. In each independent experiment, the desmoplakin band for each treatment condition was normalized to the GAPDH signal. The experiment was repeated three times and the mean values from these experiments ± SEM are reported. Statistical significance was determined using t-test. * P
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    Differential display and Northern blot analysis of R5020 and epidermal growth factor (EGF) regulation of desmoplakin I (DPI) and II (DPII) in T47D cells. (a) Autoradiography of a representative differential display RT-PCR acrylamide gel. 33 P-labeled DNA fragments generated by RT-PCR from RNAs of control T47D cells (lane 1), 10 nmol/l R5020 treatment (lane 2), 10 nmol/l EGF treatment (lane 3), 10 nmol/l R5020 plus 10 nmol/l EGF treatment (lane 4). Each lane contains 5 μL PCR mixture. (A) 1:20 dilution of RT-PCR cDNA. (B) 1:40 dilution of RT-PCR cDNA. The arrow indicates desmoplakin (DP). Molecular weight standards of 0.65, 0.42, and 0.41 kb are indicated in lane 5. (b) Photograph of a representative Northern blot analysis of DPI and DPII, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA expression in T47D cells. Lane 1: control T47D cells were left untreated for 48 hours. Matched sister colonies of T47D cells received 10nmol/l R5020 (lane 2), 10 nmol/l EGF (lane 3), or 10 nmol/l R5020 plus 10 nmol/l EGF for 48 hours (lane 4). Ten micrograms of total RNA was subjected to Northern blot analysis as described in the Methods section. (c) The Northern blot films were scanned. The graphs indicate the fold difference of desmoplakin expression compared vehicle (set at a value of 1) following measurement of the band intensities. In each independent experiment, the desmoplakin band for each treatment condition was normalized to the GAPDH signal. The experiment was repeated three times and the mean values from these experiments ± SEM are reported. Statistical significance was determined using t-test. * P

    Journal: Breast Cancer Research

    Article Title: Epidermal growth factor suppresses induction by progestin of the adhesion protein desmoplakin in T47D breast cancer cells

    doi: 10.1186/bcr780

    Figure Lengend Snippet: Differential display and Northern blot analysis of R5020 and epidermal growth factor (EGF) regulation of desmoplakin I (DPI) and II (DPII) in T47D cells. (a) Autoradiography of a representative differential display RT-PCR acrylamide gel. 33 P-labeled DNA fragments generated by RT-PCR from RNAs of control T47D cells (lane 1), 10 nmol/l R5020 treatment (lane 2), 10 nmol/l EGF treatment (lane 3), 10 nmol/l R5020 plus 10 nmol/l EGF treatment (lane 4). Each lane contains 5 μL PCR mixture. (A) 1:20 dilution of RT-PCR cDNA. (B) 1:40 dilution of RT-PCR cDNA. The arrow indicates desmoplakin (DP). Molecular weight standards of 0.65, 0.42, and 0.41 kb are indicated in lane 5. (b) Photograph of a representative Northern blot analysis of DPI and DPII, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA expression in T47D cells. Lane 1: control T47D cells were left untreated for 48 hours. Matched sister colonies of T47D cells received 10nmol/l R5020 (lane 2), 10 nmol/l EGF (lane 3), or 10 nmol/l R5020 plus 10 nmol/l EGF for 48 hours (lane 4). Ten micrograms of total RNA was subjected to Northern blot analysis as described in the Methods section. (c) The Northern blot films were scanned. The graphs indicate the fold difference of desmoplakin expression compared vehicle (set at a value of 1) following measurement of the band intensities. In each independent experiment, the desmoplakin band for each treatment condition was normalized to the GAPDH signal. The experiment was repeated three times and the mean values from these experiments ± SEM are reported. Statistical significance was determined using t-test. * P

    Article Snippet: Blot hybridization was done in 5 × SSC, 50% formamide, 2 × Denhardt, 20 mmol/l Na2 HPO4 , 0.1% SDS, 10% dextran sulfate, and 100 μg/ml salmon testis DNA, with the cDNA probe labeled with [32 P]-dCTP using Random Primers DNA Labeling Kit (Gibco BRL, Grand Island, NY, USA).

    Techniques: Northern Blot, Autoradiography, Reverse Transcription Polymerase Chain Reaction, Acrylamide Gel Assay, Labeling, Generated, Polymerase Chain Reaction, Molecular Weight, Expressing