oligo dt primers Thermo Fisher Search Results


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  • 95
    Qiagen oligo dt primers
    Oligo Dt Primers, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 1199 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 95 stars, based on 1199 article reviews
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    oligo dt primers - by Bioz Stars, 2020-02
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    95
    Millipore oligo
    Oligo, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 1223 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 95 stars, based on 1223 article reviews
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    90
    Thermo Fisher oligodeoxythymidylic acid primer
    Oligodeoxythymidylic Acid Primer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 669 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 669 article reviews
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    90
    Thermo Fisher oligo
    Oligo, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 26412 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher oligos
    BAX knockdown by <t>siRNA</t> mimics promotes tumor growth in vivo. siNC and siBAX <t>oligos</t> were injected into A431 cell xenografts every three days. ( A ) Loss of BAX promotes subcutaneous tumor growth in a mouse xenograft model. Tumor volumes (mm 3 ) were plotted according to days. Tumor volume statistical data represent the average of three independent experiments ± s.d., respectively; ( B ) the mice were sacrificed at the end of the experiment and images taken along with the dissected tumors from three representative mice are shown. White arrows indicate the siNC-treated xenografts, while black arrows indicate siBAX-treated xenografts; ( C ) the expression of BAX was measured in the dissected tumors by qRT-PCR. qRT-PCR statistical data represent the average of three independent experiments ± s.d. Expression folds are shown with respect to NC where normalized copy numbers were set to 1; ( D ) the protein expression of BAX was detected in xenografts after siBAX treatment by Western blot; ( E ) histopathology analysis (IHC staining) of BAX on tumor sections. The quantification was done by counting positively-stained cells from 20 randomly-chosen fields from a total of five sections per tumor. Magnification, 400×, Scale bars, 50 µm; ( F ) TUNEL assay of apoptosis on tumor sections after BAX depletion. The quantification was done by counting positively-stained signals from 20 randomly-chosen fields from a total of five sections per tumor. Magnification, 400×, Scale bars, 50 µm. ** p
    Oligos, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 2907 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher oligo dt 12
    BAX knockdown by <t>siRNA</t> mimics promotes tumor growth in vivo. siNC and siBAX <t>oligos</t> were injected into A431 cell xenografts every three days. ( A ) Loss of BAX promotes subcutaneous tumor growth in a mouse xenograft model. Tumor volumes (mm 3 ) were plotted according to days. Tumor volume statistical data represent the average of three independent experiments ± s.d., respectively; ( B ) the mice were sacrificed at the end of the experiment and images taken along with the dissected tumors from three representative mice are shown. White arrows indicate the siNC-treated xenografts, while black arrows indicate siBAX-treated xenografts; ( C ) the expression of BAX was measured in the dissected tumors by qRT-PCR. qRT-PCR statistical data represent the average of three independent experiments ± s.d. Expression folds are shown with respect to NC where normalized copy numbers were set to 1; ( D ) the protein expression of BAX was detected in xenografts after siBAX treatment by Western blot; ( E ) histopathology analysis (IHC staining) of BAX on tumor sections. The quantification was done by counting positively-stained cells from 20 randomly-chosen fields from a total of five sections per tumor. Magnification, 400×, Scale bars, 50 µm; ( F ) TUNEL assay of apoptosis on tumor sections after BAX depletion. The quantification was done by counting positively-stained signals from 20 randomly-chosen fields from a total of five sections per tumor. Magnification, 400×, Scale bars, 50 µm. ** p
    Oligo Dt 12, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2404 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher oligo dt 12 18 primer
    BAX knockdown by <t>siRNA</t> mimics promotes tumor growth in vivo. siNC and siBAX <t>oligos</t> were injected into A431 cell xenografts every three days. ( A ) Loss of BAX promotes subcutaneous tumor growth in a mouse xenograft model. Tumor volumes (mm 3 ) were plotted according to days. Tumor volume statistical data represent the average of three independent experiments ± s.d., respectively; ( B ) the mice were sacrificed at the end of the experiment and images taken along with the dissected tumors from three representative mice are shown. White arrows indicate the siNC-treated xenografts, while black arrows indicate siBAX-treated xenografts; ( C ) the expression of BAX was measured in the dissected tumors by qRT-PCR. qRT-PCR statistical data represent the average of three independent experiments ± s.d. Expression folds are shown with respect to NC where normalized copy numbers were set to 1; ( D ) the protein expression of BAX was detected in xenografts after siBAX treatment by Western blot; ( E ) histopathology analysis (IHC staining) of BAX on tumor sections. The quantification was done by counting positively-stained cells from 20 randomly-chosen fields from a total of five sections per tumor. Magnification, 400×, Scale bars, 50 µm; ( F ) TUNEL assay of apoptosis on tumor sections after BAX depletion. The quantification was done by counting positively-stained signals from 20 randomly-chosen fields from a total of five sections per tumor. Magnification, 400×, Scale bars, 50 µm. ** p
    Oligo Dt 12 18 Primer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 489 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Thermo Fisher oligo dt t7 primer
    BAX knockdown by <t>siRNA</t> mimics promotes tumor growth in vivo. siNC and siBAX <t>oligos</t> were injected into A431 cell xenografts every three days. ( A ) Loss of BAX promotes subcutaneous tumor growth in a mouse xenograft model. Tumor volumes (mm 3 ) were plotted according to days. Tumor volume statistical data represent the average of three independent experiments ± s.d., respectively; ( B ) the mice were sacrificed at the end of the experiment and images taken along with the dissected tumors from three representative mice are shown. White arrows indicate the siNC-treated xenografts, while black arrows indicate siBAX-treated xenografts; ( C ) the expression of BAX was measured in the dissected tumors by qRT-PCR. qRT-PCR statistical data represent the average of three independent experiments ± s.d. Expression folds are shown with respect to NC where normalized copy numbers were set to 1; ( D ) the protein expression of BAX was detected in xenografts after siBAX treatment by Western blot; ( E ) histopathology analysis (IHC staining) of BAX on tumor sections. The quantification was done by counting positively-stained cells from 20 randomly-chosen fields from a total of five sections per tumor. Magnification, 400×, Scale bars, 50 µm; ( F ) TUNEL assay of apoptosis on tumor sections after BAX depletion. The quantification was done by counting positively-stained signals from 20 randomly-chosen fields from a total of five sections per tumor. Magnification, 400×, Scale bars, 50 µm. ** p
    Oligo Dt T7 Primer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 99 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 89 stars, based on 99 article reviews
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    91
    Thermo Fisher generacer oligo dt primer
    BAX knockdown by <t>siRNA</t> mimics promotes tumor growth in vivo. siNC and siBAX <t>oligos</t> were injected into A431 cell xenografts every three days. ( A ) Loss of BAX promotes subcutaneous tumor growth in a mouse xenograft model. Tumor volumes (mm 3 ) were plotted according to days. Tumor volume statistical data represent the average of three independent experiments ± s.d., respectively; ( B ) the mice were sacrificed at the end of the experiment and images taken along with the dissected tumors from three representative mice are shown. White arrows indicate the siNC-treated xenografts, while black arrows indicate siBAX-treated xenografts; ( C ) the expression of BAX was measured in the dissected tumors by qRT-PCR. qRT-PCR statistical data represent the average of three independent experiments ± s.d. Expression folds are shown with respect to NC where normalized copy numbers were set to 1; ( D ) the protein expression of BAX was detected in xenografts after siBAX treatment by Western blot; ( E ) histopathology analysis (IHC staining) of BAX on tumor sections. The quantification was done by counting positively-stained cells from 20 randomly-chosen fields from a total of five sections per tumor. Magnification, 400×, Scale bars, 50 µm; ( F ) TUNEL assay of apoptosis on tumor sections after BAX depletion. The quantification was done by counting positively-stained signals from 20 randomly-chosen fields from a total of five sections per tumor. Magnification, 400×, Scale bars, 50 µm. ** p
    Generacer Oligo Dt Primer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 116 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher oligo dt random primers
    BAX knockdown by <t>siRNA</t> mimics promotes tumor growth in vivo. siNC and siBAX <t>oligos</t> were injected into A431 cell xenografts every three days. ( A ) Loss of BAX promotes subcutaneous tumor growth in a mouse xenograft model. Tumor volumes (mm 3 ) were plotted according to days. Tumor volume statistical data represent the average of three independent experiments ± s.d., respectively; ( B ) the mice were sacrificed at the end of the experiment and images taken along with the dissected tumors from three representative mice are shown. White arrows indicate the siNC-treated xenografts, while black arrows indicate siBAX-treated xenografts; ( C ) the expression of BAX was measured in the dissected tumors by qRT-PCR. qRT-PCR statistical data represent the average of three independent experiments ± s.d. Expression folds are shown with respect to NC where normalized copy numbers were set to 1; ( D ) the protein expression of BAX was detected in xenografts after siBAX treatment by Western blot; ( E ) histopathology analysis (IHC staining) of BAX on tumor sections. The quantification was done by counting positively-stained cells from 20 randomly-chosen fields from a total of five sections per tumor. Magnification, 400×, Scale bars, 50 µm; ( F ) TUNEL assay of apoptosis on tumor sections after BAX depletion. The quantification was done by counting positively-stained signals from 20 randomly-chosen fields from a total of five sections per tumor. Magnification, 400×, Scale bars, 50 µm. ** p
    Oligo Dt Random Primers, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 94 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Thermo Fisher oligo dt t8 primer
    BAX knockdown by <t>siRNA</t> mimics promotes tumor growth in vivo. siNC and siBAX <t>oligos</t> were injected into A431 cell xenografts every three days. ( A ) Loss of BAX promotes subcutaneous tumor growth in a mouse xenograft model. Tumor volumes (mm 3 ) were plotted according to days. Tumor volume statistical data represent the average of three independent experiments ± s.d., respectively; ( B ) the mice were sacrificed at the end of the experiment and images taken along with the dissected tumors from three representative mice are shown. White arrows indicate the siNC-treated xenografts, while black arrows indicate siBAX-treated xenografts; ( C ) the expression of BAX was measured in the dissected tumors by qRT-PCR. qRT-PCR statistical data represent the average of three independent experiments ± s.d. Expression folds are shown with respect to NC where normalized copy numbers were set to 1; ( D ) the protein expression of BAX was detected in xenografts after siBAX treatment by Western blot; ( E ) histopathology analysis (IHC staining) of BAX on tumor sections. The quantification was done by counting positively-stained cells from 20 randomly-chosen fields from a total of five sections per tumor. Magnification, 400×, Scale bars, 50 µm; ( F ) TUNEL assay of apoptosis on tumor sections after BAX depletion. The quantification was done by counting positively-stained signals from 20 randomly-chosen fields from a total of five sections per tumor. Magnification, 400×, Scale bars, 50 µm. ** p
    Oligo Dt T8 Primer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Thermo Fisher oligodeoxythymidylic acid adapter primer
    BAX knockdown by <t>siRNA</t> mimics promotes tumor growth in vivo. siNC and siBAX <t>oligos</t> were injected into A431 cell xenografts every three days. ( A ) Loss of BAX promotes subcutaneous tumor growth in a mouse xenograft model. Tumor volumes (mm 3 ) were plotted according to days. Tumor volume statistical data represent the average of three independent experiments ± s.d., respectively; ( B ) the mice were sacrificed at the end of the experiment and images taken along with the dissected tumors from three representative mice are shown. White arrows indicate the siNC-treated xenografts, while black arrows indicate siBAX-treated xenografts; ( C ) the expression of BAX was measured in the dissected tumors by qRT-PCR. qRT-PCR statistical data represent the average of three independent experiments ± s.d. Expression folds are shown with respect to NC where normalized copy numbers were set to 1; ( D ) the protein expression of BAX was detected in xenografts after siBAX treatment by Western blot; ( E ) histopathology analysis (IHC staining) of BAX on tumor sections. The quantification was done by counting positively-stained cells from 20 randomly-chosen fields from a total of five sections per tumor. Magnification, 400×, Scale bars, 50 µm; ( F ) TUNEL assay of apoptosis on tumor sections after BAX depletion. The quantification was done by counting positively-stained signals from 20 randomly-chosen fields from a total of five sections per tumor. Magnification, 400×, Scale bars, 50 µm. ** p
    Oligodeoxythymidylic Acid Adapter Primer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Thermo Fisher oligo dt primer dt15
    BAX knockdown by <t>siRNA</t> mimics promotes tumor growth in vivo. siNC and siBAX <t>oligos</t> were injected into A431 cell xenografts every three days. ( A ) Loss of BAX promotes subcutaneous tumor growth in a mouse xenograft model. Tumor volumes (mm 3 ) were plotted according to days. Tumor volume statistical data represent the average of three independent experiments ± s.d., respectively; ( B ) the mice were sacrificed at the end of the experiment and images taken along with the dissected tumors from three representative mice are shown. White arrows indicate the siNC-treated xenografts, while black arrows indicate siBAX-treated xenografts; ( C ) the expression of BAX was measured in the dissected tumors by qRT-PCR. qRT-PCR statistical data represent the average of three independent experiments ± s.d. Expression folds are shown with respect to NC where normalized copy numbers were set to 1; ( D ) the protein expression of BAX was detected in xenografts after siBAX treatment by Western blot; ( E ) histopathology analysis (IHC staining) of BAX on tumor sections. The quantification was done by counting positively-stained cells from 20 randomly-chosen fields from a total of five sections per tumor. Magnification, 400×, Scale bars, 50 µm; ( F ) TUNEL assay of apoptosis on tumor sections after BAX depletion. The quantification was done by counting positively-stained signals from 20 randomly-chosen fields from a total of five sections per tumor. Magnification, 400×, Scale bars, 50 µm. ** p
    Oligo Dt Primer Dt15, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    82
    Thermo Fisher t7 oligo dt promoter primer
    BAX knockdown by <t>siRNA</t> mimics promotes tumor growth in vivo. siNC and siBAX <t>oligos</t> were injected into A431 cell xenografts every three days. ( A ) Loss of BAX promotes subcutaneous tumor growth in a mouse xenograft model. Tumor volumes (mm 3 ) were plotted according to days. Tumor volume statistical data represent the average of three independent experiments ± s.d., respectively; ( B ) the mice were sacrificed at the end of the experiment and images taken along with the dissected tumors from three representative mice are shown. White arrows indicate the siNC-treated xenografts, while black arrows indicate siBAX-treated xenografts; ( C ) the expression of BAX was measured in the dissected tumors by qRT-PCR. qRT-PCR statistical data represent the average of three independent experiments ± s.d. Expression folds are shown with respect to NC where normalized copy numbers were set to 1; ( D ) the protein expression of BAX was detected in xenografts after siBAX treatment by Western blot; ( E ) histopathology analysis (IHC staining) of BAX on tumor sections. The quantification was done by counting positively-stained cells from 20 randomly-chosen fields from a total of five sections per tumor. Magnification, 400×, Scale bars, 50 µm; ( F ) TUNEL assay of apoptosis on tumor sections after BAX depletion. The quantification was done by counting positively-stained signals from 20 randomly-chosen fields from a total of five sections per tumor. Magnification, 400×, Scale bars, 50 µm. ** p
    T7 Oligo Dt Promoter Primer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 82/100, based on 98 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 82 stars, based on 98 article reviews
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    90
    Thermo Fisher dynabeads
    BAX knockdown by <t>siRNA</t> mimics promotes tumor growth in vivo. siNC and siBAX <t>oligos</t> were injected into A431 cell xenografts every three days. ( A ) Loss of BAX promotes subcutaneous tumor growth in a mouse xenograft model. Tumor volumes (mm 3 ) were plotted according to days. Tumor volume statistical data represent the average of three independent experiments ± s.d., respectively; ( B ) the mice were sacrificed at the end of the experiment and images taken along with the dissected tumors from three representative mice are shown. White arrows indicate the siNC-treated xenografts, while black arrows indicate siBAX-treated xenografts; ( C ) the expression of BAX was measured in the dissected tumors by qRT-PCR. qRT-PCR statistical data represent the average of three independent experiments ± s.d. Expression folds are shown with respect to NC where normalized copy numbers were set to 1; ( D ) the protein expression of BAX was detected in xenografts after siBAX treatment by Western blot; ( E ) histopathology analysis (IHC staining) of BAX on tumor sections. The quantification was done by counting positively-stained cells from 20 randomly-chosen fields from a total of five sections per tumor. Magnification, 400×, Scale bars, 50 µm; ( F ) TUNEL assay of apoptosis on tumor sections after BAX depletion. The quantification was done by counting positively-stained signals from 20 randomly-chosen fields from a total of five sections per tumor. Magnification, 400×, Scale bars, 50 µm. ** p
    Dynabeads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 13467 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Thermo Fisher retroscript kit oligo dt primers
    BAX knockdown by <t>siRNA</t> mimics promotes tumor growth in vivo. siNC and siBAX <t>oligos</t> were injected into A431 cell xenografts every three days. ( A ) Loss of BAX promotes subcutaneous tumor growth in a mouse xenograft model. Tumor volumes (mm 3 ) were plotted according to days. Tumor volume statistical data represent the average of three independent experiments ± s.d., respectively; ( B ) the mice were sacrificed at the end of the experiment and images taken along with the dissected tumors from three representative mice are shown. White arrows indicate the siNC-treated xenografts, while black arrows indicate siBAX-treated xenografts; ( C ) the expression of BAX was measured in the dissected tumors by qRT-PCR. qRT-PCR statistical data represent the average of three independent experiments ± s.d. Expression folds are shown with respect to NC where normalized copy numbers were set to 1; ( D ) the protein expression of BAX was detected in xenografts after siBAX treatment by Western blot; ( E ) histopathology analysis (IHC staining) of BAX on tumor sections. The quantification was done by counting positively-stained cells from 20 randomly-chosen fields from a total of five sections per tumor. Magnification, 400×, Scale bars, 50 µm; ( F ) TUNEL assay of apoptosis on tumor sections after BAX depletion. The quantification was done by counting positively-stained signals from 20 randomly-chosen fields from a total of five sections per tumor. Magnification, 400×, Scale bars, 50 µm. ** p
    Retroscript Kit Oligo Dt Primers, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    87
    Thermo Fisher genechip t7 oligo dt promoter primer kit
    BAX knockdown by <t>siRNA</t> mimics promotes tumor growth in vivo. siNC and siBAX <t>oligos</t> were injected into A431 cell xenografts every three days. ( A ) Loss of BAX promotes subcutaneous tumor growth in a mouse xenograft model. Tumor volumes (mm 3 ) were plotted according to days. Tumor volume statistical data represent the average of three independent experiments ± s.d., respectively; ( B ) the mice were sacrificed at the end of the experiment and images taken along with the dissected tumors from three representative mice are shown. White arrows indicate the siNC-treated xenografts, while black arrows indicate siBAX-treated xenografts; ( C ) the expression of BAX was measured in the dissected tumors by qRT-PCR. qRT-PCR statistical data represent the average of three independent experiments ± s.d. Expression folds are shown with respect to NC where normalized copy numbers were set to 1; ( D ) the protein expression of BAX was detected in xenografts after siBAX treatment by Western blot; ( E ) histopathology analysis (IHC staining) of BAX on tumor sections. The quantification was done by counting positively-stained cells from 20 randomly-chosen fields from a total of five sections per tumor. Magnification, 400×, Scale bars, 50 µm; ( F ) TUNEL assay of apoptosis on tumor sections after BAX depletion. The quantification was done by counting positively-stained signals from 20 randomly-chosen fields from a total of five sections per tumor. Magnification, 400×, Scale bars, 50 µm. ** p
    Genechip T7 Oligo Dt Promoter Primer Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    82
    Thermo Fisher mrna transcription oligo dt primers
    BAX knockdown by <t>siRNA</t> mimics promotes tumor growth in vivo. siNC and siBAX <t>oligos</t> were injected into A431 cell xenografts every three days. ( A ) Loss of BAX promotes subcutaneous tumor growth in a mouse xenograft model. Tumor volumes (mm 3 ) were plotted according to days. Tumor volume statistical data represent the average of three independent experiments ± s.d., respectively; ( B ) the mice were sacrificed at the end of the experiment and images taken along with the dissected tumors from three representative mice are shown. White arrows indicate the siNC-treated xenografts, while black arrows indicate siBAX-treated xenografts; ( C ) the expression of BAX was measured in the dissected tumors by qRT-PCR. qRT-PCR statistical data represent the average of three independent experiments ± s.d. Expression folds are shown with respect to NC where normalized copy numbers were set to 1; ( D ) the protein expression of BAX was detected in xenografts after siBAX treatment by Western blot; ( E ) histopathology analysis (IHC staining) of BAX on tumor sections. The quantification was done by counting positively-stained cells from 20 randomly-chosen fields from a total of five sections per tumor. Magnification, 400×, Scale bars, 50 µm; ( F ) TUNEL assay of apoptosis on tumor sections after BAX depletion. The quantification was done by counting positively-stained signals from 20 randomly-chosen fields from a total of five sections per tumor. Magnification, 400×, Scale bars, 50 µm. ** p
    Mrna Transcription Oligo Dt Primers, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 82/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Thermo Fisher oligo dt primed reverse transcriptase
    BAX knockdown by <t>siRNA</t> mimics promotes tumor growth in vivo. siNC and siBAX <t>oligos</t> were injected into A431 cell xenografts every three days. ( A ) Loss of BAX promotes subcutaneous tumor growth in a mouse xenograft model. Tumor volumes (mm 3 ) were plotted according to days. Tumor volume statistical data represent the average of three independent experiments ± s.d., respectively; ( B ) the mice were sacrificed at the end of the experiment and images taken along with the dissected tumors from three representative mice are shown. White arrows indicate the siNC-treated xenografts, while black arrows indicate siBAX-treated xenografts; ( C ) the expression of BAX was measured in the dissected tumors by qRT-PCR. qRT-PCR statistical data represent the average of three independent experiments ± s.d. Expression folds are shown with respect to NC where normalized copy numbers were set to 1; ( D ) the protein expression of BAX was detected in xenografts after siBAX treatment by Western blot; ( E ) histopathology analysis (IHC staining) of BAX on tumor sections. The quantification was done by counting positively-stained cells from 20 randomly-chosen fields from a total of five sections per tumor. Magnification, 400×, Scale bars, 50 µm; ( F ) TUNEL assay of apoptosis on tumor sections after BAX depletion. The quantification was done by counting positively-stained signals from 20 randomly-chosen fields from a total of five sections per tumor. Magnification, 400×, Scale bars, 50 µm. ** p
    Oligo Dt Primed Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher oligo dt 15 primer solution
    BAX knockdown by <t>siRNA</t> mimics promotes tumor growth in vivo. siNC and siBAX <t>oligos</t> were injected into A431 cell xenografts every three days. ( A ) Loss of BAX promotes subcutaneous tumor growth in a mouse xenograft model. Tumor volumes (mm 3 ) were plotted according to days. Tumor volume statistical data represent the average of three independent experiments ± s.d., respectively; ( B ) the mice were sacrificed at the end of the experiment and images taken along with the dissected tumors from three representative mice are shown. White arrows indicate the siNC-treated xenografts, while black arrows indicate siBAX-treated xenografts; ( C ) the expression of BAX was measured in the dissected tumors by qRT-PCR. qRT-PCR statistical data represent the average of three independent experiments ± s.d. Expression folds are shown with respect to NC where normalized copy numbers were set to 1; ( D ) the protein expression of BAX was detected in xenografts after siBAX treatment by Western blot; ( E ) histopathology analysis (IHC staining) of BAX on tumor sections. The quantification was done by counting positively-stained cells from 20 randomly-chosen fields from a total of five sections per tumor. Magnification, 400×, Scale bars, 50 µm; ( F ) TUNEL assay of apoptosis on tumor sections after BAX depletion. The quantification was done by counting positively-stained signals from 20 randomly-chosen fields from a total of five sections per tumor. Magnification, 400×, Scale bars, 50 µm. ** p
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    BAX knockdown by <t>siRNA</t> mimics promotes tumor growth in vivo. siNC and siBAX <t>oligos</t> were injected into A431 cell xenografts every three days. ( A ) Loss of BAX promotes subcutaneous tumor growth in a mouse xenograft model. Tumor volumes (mm 3 ) were plotted according to days. Tumor volume statistical data represent the average of three independent experiments ± s.d., respectively; ( B ) the mice were sacrificed at the end of the experiment and images taken along with the dissected tumors from three representative mice are shown. White arrows indicate the siNC-treated xenografts, while black arrows indicate siBAX-treated xenografts; ( C ) the expression of BAX was measured in the dissected tumors by qRT-PCR. qRT-PCR statistical data represent the average of three independent experiments ± s.d. Expression folds are shown with respect to NC where normalized copy numbers were set to 1; ( D ) the protein expression of BAX was detected in xenografts after siBAX treatment by Western blot; ( E ) histopathology analysis (IHC staining) of BAX on tumor sections. The quantification was done by counting positively-stained cells from 20 randomly-chosen fields from a total of five sections per tumor. Magnification, 400×, Scale bars, 50 µm; ( F ) TUNEL assay of apoptosis on tumor sections after BAX depletion. The quantification was done by counting positively-stained signals from 20 randomly-chosen fields from a total of five sections per tumor. Magnification, 400×, Scale bars, 50 µm. ** p
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    BAX knockdown by <t>siRNA</t> mimics promotes tumor growth in vivo. siNC and siBAX <t>oligos</t> were injected into A431 cell xenografts every three days. ( A ) Loss of BAX promotes subcutaneous tumor growth in a mouse xenograft model. Tumor volumes (mm 3 ) were plotted according to days. Tumor volume statistical data represent the average of three independent experiments ± s.d., respectively; ( B ) the mice were sacrificed at the end of the experiment and images taken along with the dissected tumors from three representative mice are shown. White arrows indicate the siNC-treated xenografts, while black arrows indicate siBAX-treated xenografts; ( C ) the expression of BAX was measured in the dissected tumors by qRT-PCR. qRT-PCR statistical data represent the average of three independent experiments ± s.d. Expression folds are shown with respect to NC where normalized copy numbers were set to 1; ( D ) the protein expression of BAX was detected in xenografts after siBAX treatment by Western blot; ( E ) histopathology analysis (IHC staining) of BAX on tumor sections. The quantification was done by counting positively-stained cells from 20 randomly-chosen fields from a total of five sections per tumor. Magnification, 400×, Scale bars, 50 µm; ( F ) TUNEL assay of apoptosis on tumor sections after BAX depletion. The quantification was done by counting positively-stained signals from 20 randomly-chosen fields from a total of five sections per tumor. Magnification, 400×, Scale bars, 50 µm. ** p
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    BAX knockdown by <t>siRNA</t> mimics promotes tumor growth in vivo. siNC and siBAX <t>oligos</t> were injected into A431 cell xenografts every three days. ( A ) Loss of BAX promotes subcutaneous tumor growth in a mouse xenograft model. Tumor volumes (mm 3 ) were plotted according to days. Tumor volume statistical data represent the average of three independent experiments ± s.d., respectively; ( B ) the mice were sacrificed at the end of the experiment and images taken along with the dissected tumors from three representative mice are shown. White arrows indicate the siNC-treated xenografts, while black arrows indicate siBAX-treated xenografts; ( C ) the expression of BAX was measured in the dissected tumors by qRT-PCR. qRT-PCR statistical data represent the average of three independent experiments ± s.d. Expression folds are shown with respect to NC where normalized copy numbers were set to 1; ( D ) the protein expression of BAX was detected in xenografts after siBAX treatment by Western blot; ( E ) histopathology analysis (IHC staining) of BAX on tumor sections. The quantification was done by counting positively-stained cells from 20 randomly-chosen fields from a total of five sections per tumor. Magnification, 400×, Scale bars, 50 µm; ( F ) TUNEL assay of apoptosis on tumor sections after BAX depletion. The quantification was done by counting positively-stained signals from 20 randomly-chosen fields from a total of five sections per tumor. Magnification, 400×, Scale bars, 50 µm. ** p
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    BAX knockdown by <t>siRNA</t> mimics promotes tumor growth in vivo. siNC and siBAX <t>oligos</t> were injected into A431 cell xenografts every three days. ( A ) Loss of BAX promotes subcutaneous tumor growth in a mouse xenograft model. Tumor volumes (mm 3 ) were plotted according to days. Tumor volume statistical data represent the average of three independent experiments ± s.d., respectively; ( B ) the mice were sacrificed at the end of the experiment and images taken along with the dissected tumors from three representative mice are shown. White arrows indicate the siNC-treated xenografts, while black arrows indicate siBAX-treated xenografts; ( C ) the expression of BAX was measured in the dissected tumors by qRT-PCR. qRT-PCR statistical data represent the average of three independent experiments ± s.d. Expression folds are shown with respect to NC where normalized copy numbers were set to 1; ( D ) the protein expression of BAX was detected in xenografts after siBAX treatment by Western blot; ( E ) histopathology analysis (IHC staining) of BAX on tumor sections. The quantification was done by counting positively-stained cells from 20 randomly-chosen fields from a total of five sections per tumor. Magnification, 400×, Scale bars, 50 µm; ( F ) TUNEL assay of apoptosis on tumor sections after BAX depletion. The quantification was done by counting positively-stained signals from 20 randomly-chosen fields from a total of five sections per tumor. Magnification, 400×, Scale bars, 50 µm. ** p
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    Image Search Results


    BAX knockdown by siRNA mimics promotes tumor growth in vivo. siNC and siBAX oligos were injected into A431 cell xenografts every three days. ( A ) Loss of BAX promotes subcutaneous tumor growth in a mouse xenograft model. Tumor volumes (mm 3 ) were plotted according to days. Tumor volume statistical data represent the average of three independent experiments ± s.d., respectively; ( B ) the mice were sacrificed at the end of the experiment and images taken along with the dissected tumors from three representative mice are shown. White arrows indicate the siNC-treated xenografts, while black arrows indicate siBAX-treated xenografts; ( C ) the expression of BAX was measured in the dissected tumors by qRT-PCR. qRT-PCR statistical data represent the average of three independent experiments ± s.d. Expression folds are shown with respect to NC where normalized copy numbers were set to 1; ( D ) the protein expression of BAX was detected in xenografts after siBAX treatment by Western blot; ( E ) histopathology analysis (IHC staining) of BAX on tumor sections. The quantification was done by counting positively-stained cells from 20 randomly-chosen fields from a total of five sections per tumor. Magnification, 400×, Scale bars, 50 µm; ( F ) TUNEL assay of apoptosis on tumor sections after BAX depletion. The quantification was done by counting positively-stained signals from 20 randomly-chosen fields from a total of five sections per tumor. Magnification, 400×, Scale bars, 50 µm. ** p

    Journal: International Journal of Molecular Sciences

    Article Title: Loss of BAX by miR-365 Promotes Cutaneous Squamous Cell Carcinoma Progression by Suppressing Apoptosis

    doi: 10.3390/ijms18061157

    Figure Lengend Snippet: BAX knockdown by siRNA mimics promotes tumor growth in vivo. siNC and siBAX oligos were injected into A431 cell xenografts every three days. ( A ) Loss of BAX promotes subcutaneous tumor growth in a mouse xenograft model. Tumor volumes (mm 3 ) were plotted according to days. Tumor volume statistical data represent the average of three independent experiments ± s.d., respectively; ( B ) the mice were sacrificed at the end of the experiment and images taken along with the dissected tumors from three representative mice are shown. White arrows indicate the siNC-treated xenografts, while black arrows indicate siBAX-treated xenografts; ( C ) the expression of BAX was measured in the dissected tumors by qRT-PCR. qRT-PCR statistical data represent the average of three independent experiments ± s.d. Expression folds are shown with respect to NC where normalized copy numbers were set to 1; ( D ) the protein expression of BAX was detected in xenografts after siBAX treatment by Western blot; ( E ) histopathology analysis (IHC staining) of BAX on tumor sections. The quantification was done by counting positively-stained cells from 20 randomly-chosen fields from a total of five sections per tumor. Magnification, 400×, Scale bars, 50 µm; ( F ) TUNEL assay of apoptosis on tumor sections after BAX depletion. The quantification was done by counting positively-stained signals from 20 randomly-chosen fields from a total of five sections per tumor. Magnification, 400×, Scale bars, 50 µm. ** p

    Article Snippet: Oligos were prepared by pre-incubating 3 nM siRNA per mouse with Lipofectamine 2000 (Life Technologies) for 15 min; injections were made using a final volume of 50 µL in serum free DMEM (Life Technologies).

    Techniques: In Vivo, Injection, Mouse Assay, Expressing, Quantitative RT-PCR, Western Blot, Histopathology, Immunohistochemistry, Staining, TUNEL Assay