oligo dt Thermo Fisher Search Results


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  • 99
    Thermo Fisher oligo dt
    Spearman's rank correlation tree of the gene expression patterns from amplified and unamplified RNA. RNA was amplified from 500, 50 and 5 ng total RNA using the <t>oligo-dT</t> and <t>ADP</t> methods. All M. tuberculosis H37Rv genes were clustered using median expression ratios from 2–6 replicate hybridisations.
    Oligo Dt, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 26271 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Thermo Fisher oligo dt dynabeads thermo fisher
    Spearman's rank correlation tree of the gene expression patterns from amplified and unamplified RNA. RNA was amplified from 500, 50 and 5 ng total RNA using the <t>oligo-dT</t> and <t>ADP</t> methods. All M. tuberculosis H37Rv genes were clustered using median expression ratios from 2–6 replicate hybridisations.
    Oligo Dt Dynabeads Thermo Fisher, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Qiagen oligo dt primers
    Spearman's rank correlation tree of the gene expression patterns from amplified and unamplified RNA. RNA was amplified from 500, 50 and 5 ng total RNA using the <t>oligo-dT</t> and <t>ADP</t> methods. All M. tuberculosis H37Rv genes were clustered using median expression ratios from 2–6 replicate hybridisations.
    Oligo Dt Primers, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1677 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/oligo dt primers/product/Qiagen
    Average 99 stars, based on 1677 article reviews
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    93
    Thermo Fisher oligo dt 12 18 primer thermo fisher scientific
    Spearman's rank correlation tree of the gene expression patterns from amplified and unamplified RNA. RNA was amplified from 500, 50 and 5 ng total RNA using the <t>oligo-dT</t> and <t>ADP</t> methods. All M. tuberculosis H37Rv genes were clustered using median expression ratios from 2–6 replicate hybridisations.
    Oligo Dt 12 18 Primer Thermo Fisher Scientific, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 34 article reviews
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    93
    Thermo Fisher oligo dt 20
    Spearman's rank correlation tree of the gene expression patterns from amplified and unamplified RNA. RNA was amplified from 500, 50 and 5 ng total RNA using the <t>oligo-dT</t> and <t>ADP</t> methods. All M. tuberculosis H37Rv genes were clustered using median expression ratios from 2–6 replicate hybridisations.
    Oligo Dt 20, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1834 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/oligo dt 20/product/Thermo Fisher
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    88
    Thermo Fisher dynabeads oligo dt
    Spearman's rank correlation tree of the gene expression patterns from amplified and unamplified RNA. RNA was amplified from 500, 50 and 5 ng total RNA using the <t>oligo-dT</t> and <t>ADP</t> methods. All M. tuberculosis H37Rv genes were clustered using median expression ratios from 2–6 replicate hybridisations.
    Dynabeads Oligo Dt, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 211 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 88 stars, based on 211 article reviews
    Price from $9.99 to $1999.99
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    99
    Thermo Fisher oligo dt 20 primers
    Spearman's rank correlation tree of the gene expression patterns from amplified and unamplified RNA. RNA was amplified from 500, 50 and 5 ng total RNA using the <t>oligo-dT</t> and <t>ADP</t> methods. All M. tuberculosis H37Rv genes were clustered using median expression ratios from 2–6 replicate hybridisations.
    Oligo Dt 20 Primers, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 281 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 281 article reviews
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    oligo dt 20 primers - by Bioz Stars, 2020-07
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    Thermo Fisher oligo dt 25 dynabeads
    Spearman's rank correlation tree of the gene expression patterns from amplified and unamplified RNA. RNA was amplified from 500, 50 and 5 ng total RNA using the <t>oligo-dT</t> and <t>ADP</t> methods. All M. tuberculosis H37Rv genes were clustered using median expression ratios from 2–6 replicate hybridisations.
    Oligo Dt 25 Dynabeads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher oligo dt 18
    Spearman's rank correlation tree of the gene expression patterns from amplified and unamplified RNA. RNA was amplified from 500, 50 and 5 ng total RNA using the <t>oligo-dT</t> and <t>ADP</t> methods. All M. tuberculosis H37Rv genes were clustered using median expression ratios from 2–6 replicate hybridisations.
    Oligo Dt 18, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1144 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/oligo dt 18/product/Thermo Fisher
    Average 99 stars, based on 1144 article reviews
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    oligo dt 18 - by Bioz Stars, 2020-07
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    Image Search Results


    Spearman's rank correlation tree of the gene expression patterns from amplified and unamplified RNA. RNA was amplified from 500, 50 and 5 ng total RNA using the oligo-dT and ADP methods. All M. tuberculosis H37Rv genes were clustered using median expression ratios from 2–6 replicate hybridisations.

    Journal: BMC Genomics

    Article Title: Microarray analysis of defined Mycobacterium tuberculosis populations using RNA amplification strategies

    doi: 10.1186/1471-2164-9-94

    Figure Lengend Snippet: Spearman's rank correlation tree of the gene expression patterns from amplified and unamplified RNA. RNA was amplified from 500, 50 and 5 ng total RNA using the oligo-dT and ADP methods. All M. tuberculosis H37Rv genes were clustered using median expression ratios from 2–6 replicate hybridisations.

    Article Snippet: RNA amplification 50 μM M. tb amplification-directed primers (ADP) were used in the place of oligo-dT in the MessageAmp II eukaryotic amplification system (Ambion); the second priming strategy included an initial polyadenylation step (with E. coli polyA polymerase at 37°C for 15 minutes) followed by oligo-dT based amplification (MessageAmp II Bacteria, Ambion).

    Techniques: Expressing, Amplification

    The size distribution of products amplified from 500 (triangle), 50 (square), 5 ng (diamond) M. tuberculosis total RNA using oligo-dT (A) and ADP (B) amplification strategies. (C) Unamplified total mycobacterial RNA. The peaks at 25 bp represent the marker added to all samples. The size distributions were plotted using the average of 2–4 replicate amplifications. Abundance units are detailed in relative fluorescence and plotted against migration time that has been converted into a base pair (bp) estimate of product size as measured using the Agilent Bioanalyser.

    Journal: BMC Genomics

    Article Title: Microarray analysis of defined Mycobacterium tuberculosis populations using RNA amplification strategies

    doi: 10.1186/1471-2164-9-94

    Figure Lengend Snippet: The size distribution of products amplified from 500 (triangle), 50 (square), 5 ng (diamond) M. tuberculosis total RNA using oligo-dT (A) and ADP (B) amplification strategies. (C) Unamplified total mycobacterial RNA. The peaks at 25 bp represent the marker added to all samples. The size distributions were plotted using the average of 2–4 replicate amplifications. Abundance units are detailed in relative fluorescence and plotted against migration time that has been converted into a base pair (bp) estimate of product size as measured using the Agilent Bioanalyser.

    Article Snippet: RNA amplification 50 μM M. tb amplification-directed primers (ADP) were used in the place of oligo-dT in the MessageAmp II eukaryotic amplification system (Ambion); the second priming strategy included an initial polyadenylation step (with E. coli polyA polymerase at 37°C for 15 minutes) followed by oligo-dT based amplification (MessageAmp II Bacteria, Ambion).

    Techniques: Amplification, Marker, Fluorescence, Migration

    A comparison of the number of genes not detected by microarray analysis from unamplified RNA or from products generated by oligo-dT and ADP amplification methods using 500 or 50 ng starting total RNA. The number of genes below a 2 fold signal/background threshold in the RNA channel was compared between amplified and unamplified gene expression signatures as a measure of the potential transcriptome data lost during amplification.

    Journal: BMC Genomics

    Article Title: Microarray analysis of defined Mycobacterium tuberculosis populations using RNA amplification strategies

    doi: 10.1186/1471-2164-9-94

    Figure Lengend Snippet: A comparison of the number of genes not detected by microarray analysis from unamplified RNA or from products generated by oligo-dT and ADP amplification methods using 500 or 50 ng starting total RNA. The number of genes below a 2 fold signal/background threshold in the RNA channel was compared between amplified and unamplified gene expression signatures as a measure of the potential transcriptome data lost during amplification.

    Article Snippet: RNA amplification 50 μM M. tb amplification-directed primers (ADP) were used in the place of oligo-dT in the MessageAmp II eukaryotic amplification system (Ambion); the second priming strategy included an initial polyadenylation step (with E. coli polyA polymerase at 37°C for 15 minutes) followed by oligo-dT based amplification (MessageAmp II Bacteria, Ambion).

    Techniques: Microarray, Generated, Amplification, Expressing

    A Spearman's rank correlation of the 155 genes identified to be significantly differentially expressed in microaerophilic compared to aerobic M. tuberculosis growth conditions using unamplified RNA (marked Un). The mean gene expression ratios derived from unamplified RNA and from the products of 500, 50, and 5 ng amplifications using oligo-dT and ADP methods are displayed. Genes are ordered in rows, amplification conditions as columns. Red colouring indicates genes induced in microaerophilic vs. aerobic growth conditions; green colouring denotes repression.

    Journal: BMC Genomics

    Article Title: Microarray analysis of defined Mycobacterium tuberculosis populations using RNA amplification strategies

    doi: 10.1186/1471-2164-9-94

    Figure Lengend Snippet: A Spearman's rank correlation of the 155 genes identified to be significantly differentially expressed in microaerophilic compared to aerobic M. tuberculosis growth conditions using unamplified RNA (marked Un). The mean gene expression ratios derived from unamplified RNA and from the products of 500, 50, and 5 ng amplifications using oligo-dT and ADP methods are displayed. Genes are ordered in rows, amplification conditions as columns. Red colouring indicates genes induced in microaerophilic vs. aerobic growth conditions; green colouring denotes repression.

    Article Snippet: RNA amplification 50 μM M. tb amplification-directed primers (ADP) were used in the place of oligo-dT in the MessageAmp II eukaryotic amplification system (Ambion); the second priming strategy included an initial polyadenylation step (with E. coli polyA polymerase at 37°C for 15 minutes) followed by oligo-dT based amplification (MessageAmp II Bacteria, Ambion).

    Techniques: Expressing, Derivative Assay, Amplification

    Expression of FasR, FADD, and caspase 8 in PBL and various catfish LCL. Total RNA was isolated from T (G14D), cytotoxic T (TS 32.17), B (1G8, 3B11), macrophage (42TA), and fibroblast (CCO) cell lines as well as PBLs from two fish and reverse transcribed using an oligo[dT] primer. The resulting cDNA was used in PCR along with gene-specific primers. Actin was included as a positive control. The presence or absence of FasL cross-reactive proteins as determined previously by Western blotting is indicated: + 37 denotes the 37,000- M r membrane form of FasL, whereas + 15 denotes the 15,000- M r soluble form. ND denotes not detected

    Journal: Immunogenetics

    Article Title: Identification and characterization of a FasL-like protein and cDNAs encoding the channel catfish death-inducing signaling complex

    doi: 10.1007/s00251-004-0701-2

    Figure Lengend Snippet: Expression of FasR, FADD, and caspase 8 in PBL and various catfish LCL. Total RNA was isolated from T (G14D), cytotoxic T (TS 32.17), B (1G8, 3B11), macrophage (42TA), and fibroblast (CCO) cell lines as well as PBLs from two fish and reverse transcribed using an oligo[dT] primer. The resulting cDNA was used in PCR along with gene-specific primers. Actin was included as a positive control. The presence or absence of FasL cross-reactive proteins as determined previously by Western blotting is indicated: + 37 denotes the 37,000- M r membrane form of FasL, whereas + 15 denotes the 15,000- M r soluble form. ND denotes not detected

    Article Snippet: Total RNA (2.5 μg) was reverse transcribed with an oligo(dT) primer and Superscript II RNase H-minus reverse transcriptase (Invitrogen) according to the manufacturer's instructions.

    Techniques: Expressing, Isolation, Fluorescence In Situ Hybridization, Polymerase Chain Reaction, Positive Control, Western Blot

    Ferroportin is expressed in hPASMCs and regulated by hepcidin. Confluent hPASMCs were ( A ) either mock-treated or treated with 1 µg/mL hepcidin for 2.5 h and total RNA extracted using RNeasy kit, cDNA synthesised using oligo-dT primers and RT-PCR performed using SYBR green with human Fpn (ferroportin) primers and β-actin as housekeeping gene. The values were further normalised as fold changes to the control untreated cells at time zero. N = 4 ( B ) mock-treated for 24 h, cells lysed and total protein extracted, quantified by Bradford assay and 40 µg of protein separated on 10% SDS-PAGE and transferred onto nitrocellulose membranes. Western blotting was performed using Rabbit anti-Fpn as primary and Goat anti-rabbit IgG conjugated with horse-radish peroxidase as secondary antibodies. Human intestinal lysates (Abcam) were used as positive control. N = 3 ( C ) either mock-treated or treated with 1 µg/mL hepcidin for 24 h, cells lysed and total protein extracted. Fpn expression was quantitated using an ELISA kit (BlueGene Biotech) and normalised to total protein estimated by Bradford reagent. N = 4 ( D ) Confocal images of hPASMCs grown with (top panels) normal media or (bottom panels) treated with 1 µg/mL hepcidin for 20–22 h and immuno-stained with rabbit anti-Fpn antibody and goat anti-rabbit IgG secondary antibody tagged with Alexa-568. The cells were further counterstained with DAPI and images captured using Leica LSM 510 confocal microscope. Scale bar = 10 µm; N = 5. Student’s t test was performed; **p

    Journal: Scientific Reports

    Article Title: The Hepcidin/Ferroportin axis modulates proliferation of pulmonary artery smooth muscle cells

    doi: 10.1038/s41598-018-31095-0

    Figure Lengend Snippet: Ferroportin is expressed in hPASMCs and regulated by hepcidin. Confluent hPASMCs were ( A ) either mock-treated or treated with 1 µg/mL hepcidin for 2.5 h and total RNA extracted using RNeasy kit, cDNA synthesised using oligo-dT primers and RT-PCR performed using SYBR green with human Fpn (ferroportin) primers and β-actin as housekeeping gene. The values were further normalised as fold changes to the control untreated cells at time zero. N = 4 ( B ) mock-treated for 24 h, cells lysed and total protein extracted, quantified by Bradford assay and 40 µg of protein separated on 10% SDS-PAGE and transferred onto nitrocellulose membranes. Western blotting was performed using Rabbit anti-Fpn as primary and Goat anti-rabbit IgG conjugated with horse-radish peroxidase as secondary antibodies. Human intestinal lysates (Abcam) were used as positive control. N = 3 ( C ) either mock-treated or treated with 1 µg/mL hepcidin for 24 h, cells lysed and total protein extracted. Fpn expression was quantitated using an ELISA kit (BlueGene Biotech) and normalised to total protein estimated by Bradford reagent. N = 4 ( D ) Confocal images of hPASMCs grown with (top panels) normal media or (bottom panels) treated with 1 µg/mL hepcidin for 20–22 h and immuno-stained with rabbit anti-Fpn antibody and goat anti-rabbit IgG secondary antibody tagged with Alexa-568. The cells were further counterstained with DAPI and images captured using Leica LSM 510 confocal microscope. Scale bar = 10 µm; N = 5. Student’s t test was performed; **p

    Article Snippet: 0.6–1 μg of total RNA was used for reverse transcription to produce cDNA using oligo-dT primers (Invitrogen), 10 mM dNTP mix, RNasin plus (Promega) and M-MLV Reverse transcriptase enzyme (Invitrogen) according to manufacturer recommendations.

    Techniques: Reverse Transcription Polymerase Chain Reaction, SYBR Green Assay, Bradford Assay, SDS Page, Western Blot, Positive Control, Expressing, Enzyme-linked Immunosorbent Assay, Staining, Microscopy

    IL-6 increased hepcidin expression and down-regulated ferroportin in hPASMCs. Confluent hPASMCs were either mock-treated or treated with 10 ng/mL IL-6 ( A ) for 2.5 h and total RNA extracted using RNeasy kit, cDNA synthesised using oligo-dT primers and RT-PCR performed using SYBR green with human hepcidin (Hamp-1) primers and β-actin as housekeeping gene. The values were further normalised as fold changes to the control untreated cells at time zero. N = 4 ( B ) for 24 h, media supernatant collected and hepcidin secretion was quantitated using an ELISA kit (R D Systems). N = 4 ( C ) for 24 h cells lysed and total protein extracted. Ferroportin expression was quantitated using an ELISA kit (BlueGene Biotech) and normalised to total protein estimated by Bradford reagent. N = 4 ( D ) Confocal images of hPASMCs grown with (top panels) normal media or (bottom panels) treated with 10 ng/mL IL-6 for 20–22 h and immuno-stained with rabbit anti-Fpn antibody and goat anti-rabbit IgG secondary antibody tagged with Alexa-568. The cells were further counterstained with DAPI and images captured using Leica LSM 510 confocal microscope. Scale bar = 10 µm, N = 5. Student’s t test was performed; **p

    Journal: Scientific Reports

    Article Title: The Hepcidin/Ferroportin axis modulates proliferation of pulmonary artery smooth muscle cells

    doi: 10.1038/s41598-018-31095-0

    Figure Lengend Snippet: IL-6 increased hepcidin expression and down-regulated ferroportin in hPASMCs. Confluent hPASMCs were either mock-treated or treated with 10 ng/mL IL-6 ( A ) for 2.5 h and total RNA extracted using RNeasy kit, cDNA synthesised using oligo-dT primers and RT-PCR performed using SYBR green with human hepcidin (Hamp-1) primers and β-actin as housekeeping gene. The values were further normalised as fold changes to the control untreated cells at time zero. N = 4 ( B ) for 24 h, media supernatant collected and hepcidin secretion was quantitated using an ELISA kit (R D Systems). N = 4 ( C ) for 24 h cells lysed and total protein extracted. Ferroportin expression was quantitated using an ELISA kit (BlueGene Biotech) and normalised to total protein estimated by Bradford reagent. N = 4 ( D ) Confocal images of hPASMCs grown with (top panels) normal media or (bottom panels) treated with 10 ng/mL IL-6 for 20–22 h and immuno-stained with rabbit anti-Fpn antibody and goat anti-rabbit IgG secondary antibody tagged with Alexa-568. The cells were further counterstained with DAPI and images captured using Leica LSM 510 confocal microscope. Scale bar = 10 µm, N = 5. Student’s t test was performed; **p

    Article Snippet: 0.6–1 μg of total RNA was used for reverse transcription to produce cDNA using oligo-dT primers (Invitrogen), 10 mM dNTP mix, RNasin plus (Promega) and M-MLV Reverse transcriptase enzyme (Invitrogen) according to manufacturer recommendations.

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, SYBR Green Assay, Enzyme-linked Immunosorbent Assay, Staining, Microscopy