oligo Thermo Fisher Search Results


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  • 97
    Thermo Fisher oligos targeting mad2
    Oligos Targeting Mad2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Thermo Fisher oligo dt 12 18 primer thermo fisher scientific
    Oligo Dt 12 18 Primer Thermo Fisher Scientific, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher mirna oligonucleotides targeting cdh17 gene
    Mirna Oligonucleotides Targeting Cdh17 Gene, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher morpholino oligos
    Id3 is required for cell proliferation and survival of neural crest. ( A ) Neurula-stage embryos injected with <t>morpholino</t> <t>oligos</t> and subjected to double in situ hybridization with FoxD3 and Sox2 RNA probes. (Panel a ) Dorsal view of a control embryo (anterior toward left ) shows foxD3 expression (purple) intact on the side injected with control-MO (Red-Gal). (Panels b,c ) Dorsal view of an embryo (anterior toward left ) injected with Id3-MO and anterior view of another embryo injected with Id3-MO show that the neural crest marker foxD3 (purple, arrowhead) is lost on the injected side (Red-Gal), while the neural plate (border line, arrow) marked by sox2 expression (light blue) is not expanded (red dotted line) and does not replace the neural crest. The black dotted line indicates the midline. ( B ) Cell proliferation assay measured by immunostaining with antiphosphorylated histone H3 antibody in an embryo injected with Id3-MO. Dorsal view (panel a ) and magnified anterior view (panel b ) of an embryo show that mitotic cells (black dots at control side, arrowhead) are absent or decreased in the neural crest region injected with Id3-MO (arrow, Red-Gal). ( C ) Expression of cell cycle inhibitor in the embryos injected with Id3-MO. (Panel a ) p27 XicI (purple, arrow) is ectopically expressed in neural crest progenitors when Id3 is depleted (white arrow). (Panel b ) Double in situ hybridization reveals that foxD3 expression (light blue, arrowhead) in neural crest is replaced by p27 XicI expression (purple, arrow) at the side injected with Id3-MO (Red-Gal). ( D ) Differentiation markers in the embryos injected with Id3-MO. n-tubulin (panel a ) and NeuroD (panel b ) are not ectopically expressed in the neural crest at the side injected with Id3-MO at stage 13. (Panel b ) Their expression is slightly increased in trigeminal ganglia at the side injected with Id3-MO (arrow) at stage 14. ( E ) TUNEL staining in the embryos injected with Id3-MO or control-MO. (Panels a,a ′) Id3-MO-injected region (Red-Gal) of a stage 11 embryo is negative (arrow, no black dot) in TUNEL staining. (Panels b,b ′) Dorsal view of a stage 14 embryo (anterior toward left ) shows significantly increased cell death (black dots, arrow) on the neural crest region injected with Id3-MO (Red-Gal), compared with the uninjected side (arrowhead). (Panels c,c ′) Anterior view of a stage 17 embryo also shows elevated cell death (black dots, arrow) on the neural crest region injected with Id3-MO (X-gal staining, turquoise color), compared with the uninjected side (arrowhead). (Panels d,d ′) Anterior view of a stage 16 embryo injected with control-MO shows that cell death doesn't occur in the injected region (Red-Gal, arrow), compared with the uninjected side (arrowhead). The MO-injected region is magnified for each embryo in a ′- d ′. ( F ) The graph illustrating the results of TUNEL staining as percentages of TUNEL-negative (black bar) or TUNEL-positive (red bar) embryos out of the embryos injected with control-MO or Id3-MO.
    Morpholino Oligos, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2130 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    morpholino oligos - by Bioz Stars, 2020-05
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    99
    Thermo Fisher oligod
    Id3 is required for cell proliferation and survival of neural crest. ( A ) Neurula-stage embryos injected with <t>morpholino</t> <t>oligos</t> and subjected to double in situ hybridization with FoxD3 and Sox2 RNA probes. (Panel a ) Dorsal view of a control embryo (anterior toward left ) shows foxD3 expression (purple) intact on the side injected with control-MO (Red-Gal). (Panels b,c ) Dorsal view of an embryo (anterior toward left ) injected with Id3-MO and anterior view of another embryo injected with Id3-MO show that the neural crest marker foxD3 (purple, arrowhead) is lost on the injected side (Red-Gal), while the neural plate (border line, arrow) marked by sox2 expression (light blue) is not expanded (red dotted line) and does not replace the neural crest. The black dotted line indicates the midline. ( B ) Cell proliferation assay measured by immunostaining with antiphosphorylated histone H3 antibody in an embryo injected with Id3-MO. Dorsal view (panel a ) and magnified anterior view (panel b ) of an embryo show that mitotic cells (black dots at control side, arrowhead) are absent or decreased in the neural crest region injected with Id3-MO (arrow, Red-Gal). ( C ) Expression of cell cycle inhibitor in the embryos injected with Id3-MO. (Panel a ) p27 XicI (purple, arrow) is ectopically expressed in neural crest progenitors when Id3 is depleted (white arrow). (Panel b ) Double in situ hybridization reveals that foxD3 expression (light blue, arrowhead) in neural crest is replaced by p27 XicI expression (purple, arrow) at the side injected with Id3-MO (Red-Gal). ( D ) Differentiation markers in the embryos injected with Id3-MO. n-tubulin (panel a ) and NeuroD (panel b ) are not ectopically expressed in the neural crest at the side injected with Id3-MO at stage 13. (Panel b ) Their expression is slightly increased in trigeminal ganglia at the side injected with Id3-MO (arrow) at stage 14. ( E ) TUNEL staining in the embryos injected with Id3-MO or control-MO. (Panels a,a ′) Id3-MO-injected region (Red-Gal) of a stage 11 embryo is negative (arrow, no black dot) in TUNEL staining. (Panels b,b ′) Dorsal view of a stage 14 embryo (anterior toward left ) shows significantly increased cell death (black dots, arrow) on the neural crest region injected with Id3-MO (Red-Gal), compared with the uninjected side (arrowhead). (Panels c,c ′) Anterior view of a stage 17 embryo also shows elevated cell death (black dots, arrow) on the neural crest region injected with Id3-MO (X-gal staining, turquoise color), compared with the uninjected side (arrowhead). (Panels d,d ′) Anterior view of a stage 16 embryo injected with control-MO shows that cell death doesn't occur in the injected region (Red-Gal, arrow), compared with the uninjected side (arrowhead). The MO-injected region is magnified for each embryo in a ′- d ′. ( F ) The graph illustrating the results of TUNEL staining as percentages of TUNEL-negative (black bar) or TUNEL-positive (red bar) embryos out of the embryos injected with control-MO or Id3-MO.
    Oligod, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 94 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher block it fluorescent oligomer
    Id3 is required for cell proliferation and survival of neural crest. ( A ) Neurula-stage embryos injected with <t>morpholino</t> <t>oligos</t> and subjected to double in situ hybridization with FoxD3 and Sox2 RNA probes. (Panel a ) Dorsal view of a control embryo (anterior toward left ) shows foxD3 expression (purple) intact on the side injected with control-MO (Red-Gal). (Panels b,c ) Dorsal view of an embryo (anterior toward left ) injected with Id3-MO and anterior view of another embryo injected with Id3-MO show that the neural crest marker foxD3 (purple, arrowhead) is lost on the injected side (Red-Gal), while the neural plate (border line, arrow) marked by sox2 expression (light blue) is not expanded (red dotted line) and does not replace the neural crest. The black dotted line indicates the midline. ( B ) Cell proliferation assay measured by immunostaining with antiphosphorylated histone H3 antibody in an embryo injected with Id3-MO. Dorsal view (panel a ) and magnified anterior view (panel b ) of an embryo show that mitotic cells (black dots at control side, arrowhead) are absent or decreased in the neural crest region injected with Id3-MO (arrow, Red-Gal). ( C ) Expression of cell cycle inhibitor in the embryos injected with Id3-MO. (Panel a ) p27 XicI (purple, arrow) is ectopically expressed in neural crest progenitors when Id3 is depleted (white arrow). (Panel b ) Double in situ hybridization reveals that foxD3 expression (light blue, arrowhead) in neural crest is replaced by p27 XicI expression (purple, arrow) at the side injected with Id3-MO (Red-Gal). ( D ) Differentiation markers in the embryos injected with Id3-MO. n-tubulin (panel a ) and NeuroD (panel b ) are not ectopically expressed in the neural crest at the side injected with Id3-MO at stage 13. (Panel b ) Their expression is slightly increased in trigeminal ganglia at the side injected with Id3-MO (arrow) at stage 14. ( E ) TUNEL staining in the embryos injected with Id3-MO or control-MO. (Panels a,a ′) Id3-MO-injected region (Red-Gal) of a stage 11 embryo is negative (arrow, no black dot) in TUNEL staining. (Panels b,b ′) Dorsal view of a stage 14 embryo (anterior toward left ) shows significantly increased cell death (black dots, arrow) on the neural crest region injected with Id3-MO (Red-Gal), compared with the uninjected side (arrowhead). (Panels c,c ′) Anterior view of a stage 17 embryo also shows elevated cell death (black dots, arrow) on the neural crest region injected with Id3-MO (X-gal staining, turquoise color), compared with the uninjected side (arrowhead). (Panels d,d ′) Anterior view of a stage 16 embryo injected with control-MO shows that cell death doesn't occur in the injected region (Red-Gal, arrow), compared with the uninjected side (arrowhead). The MO-injected region is magnified for each embryo in a ′- d ′. ( F ) The graph illustrating the results of TUNEL staining as percentages of TUNEL-negative (black bar) or TUNEL-positive (red bar) embryos out of the embryos injected with control-MO or Id3-MO.
    Block It Fluorescent Oligomer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher atp6v0b morpholinos
    <t>atp6v0b</t> <t>morpholinos</t> do not phenocopy blowout
    Atp6v0b Morpholinos, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Id3 is required for cell proliferation and survival of neural crest. ( A ) Neurula-stage embryos injected with morpholino oligos and subjected to double in situ hybridization with FoxD3 and Sox2 RNA probes. (Panel a ) Dorsal view of a control embryo (anterior toward left ) shows foxD3 expression (purple) intact on the side injected with control-MO (Red-Gal). (Panels b,c ) Dorsal view of an embryo (anterior toward left ) injected with Id3-MO and anterior view of another embryo injected with Id3-MO show that the neural crest marker foxD3 (purple, arrowhead) is lost on the injected side (Red-Gal), while the neural plate (border line, arrow) marked by sox2 expression (light blue) is not expanded (red dotted line) and does not replace the neural crest. The black dotted line indicates the midline. ( B ) Cell proliferation assay measured by immunostaining with antiphosphorylated histone H3 antibody in an embryo injected with Id3-MO. Dorsal view (panel a ) and magnified anterior view (panel b ) of an embryo show that mitotic cells (black dots at control side, arrowhead) are absent or decreased in the neural crest region injected with Id3-MO (arrow, Red-Gal). ( C ) Expression of cell cycle inhibitor in the embryos injected with Id3-MO. (Panel a ) p27 XicI (purple, arrow) is ectopically expressed in neural crest progenitors when Id3 is depleted (white arrow). (Panel b ) Double in situ hybridization reveals that foxD3 expression (light blue, arrowhead) in neural crest is replaced by p27 XicI expression (purple, arrow) at the side injected with Id3-MO (Red-Gal). ( D ) Differentiation markers in the embryos injected with Id3-MO. n-tubulin (panel a ) and NeuroD (panel b ) are not ectopically expressed in the neural crest at the side injected with Id3-MO at stage 13. (Panel b ) Their expression is slightly increased in trigeminal ganglia at the side injected with Id3-MO (arrow) at stage 14. ( E ) TUNEL staining in the embryos injected with Id3-MO or control-MO. (Panels a,a ′) Id3-MO-injected region (Red-Gal) of a stage 11 embryo is negative (arrow, no black dot) in TUNEL staining. (Panels b,b ′) Dorsal view of a stage 14 embryo (anterior toward left ) shows significantly increased cell death (black dots, arrow) on the neural crest region injected with Id3-MO (Red-Gal), compared with the uninjected side (arrowhead). (Panels c,c ′) Anterior view of a stage 17 embryo also shows elevated cell death (black dots, arrow) on the neural crest region injected with Id3-MO (X-gal staining, turquoise color), compared with the uninjected side (arrowhead). (Panels d,d ′) Anterior view of a stage 16 embryo injected with control-MO shows that cell death doesn't occur in the injected region (Red-Gal, arrow), compared with the uninjected side (arrowhead). The MO-injected region is magnified for each embryo in a ′- d ′. ( F ) The graph illustrating the results of TUNEL staining as percentages of TUNEL-negative (black bar) or TUNEL-positive (red bar) embryos out of the embryos injected with control-MO or Id3-MO.

    Journal: Genes & Development

    Article Title: To proliferate or to die: role of Id3 in cell cycle progression and survival of neural crest progenitors

    doi: 10.1101/gad.1257405

    Figure Lengend Snippet: Id3 is required for cell proliferation and survival of neural crest. ( A ) Neurula-stage embryos injected with morpholino oligos and subjected to double in situ hybridization with FoxD3 and Sox2 RNA probes. (Panel a ) Dorsal view of a control embryo (anterior toward left ) shows foxD3 expression (purple) intact on the side injected with control-MO (Red-Gal). (Panels b,c ) Dorsal view of an embryo (anterior toward left ) injected with Id3-MO and anterior view of another embryo injected with Id3-MO show that the neural crest marker foxD3 (purple, arrowhead) is lost on the injected side (Red-Gal), while the neural plate (border line, arrow) marked by sox2 expression (light blue) is not expanded (red dotted line) and does not replace the neural crest. The black dotted line indicates the midline. ( B ) Cell proliferation assay measured by immunostaining with antiphosphorylated histone H3 antibody in an embryo injected with Id3-MO. Dorsal view (panel a ) and magnified anterior view (panel b ) of an embryo show that mitotic cells (black dots at control side, arrowhead) are absent or decreased in the neural crest region injected with Id3-MO (arrow, Red-Gal). ( C ) Expression of cell cycle inhibitor in the embryos injected with Id3-MO. (Panel a ) p27 XicI (purple, arrow) is ectopically expressed in neural crest progenitors when Id3 is depleted (white arrow). (Panel b ) Double in situ hybridization reveals that foxD3 expression (light blue, arrowhead) in neural crest is replaced by p27 XicI expression (purple, arrow) at the side injected with Id3-MO (Red-Gal). ( D ) Differentiation markers in the embryos injected with Id3-MO. n-tubulin (panel a ) and NeuroD (panel b ) are not ectopically expressed in the neural crest at the side injected with Id3-MO at stage 13. (Panel b ) Their expression is slightly increased in trigeminal ganglia at the side injected with Id3-MO (arrow) at stage 14. ( E ) TUNEL staining in the embryos injected with Id3-MO or control-MO. (Panels a,a ′) Id3-MO-injected region (Red-Gal) of a stage 11 embryo is negative (arrow, no black dot) in TUNEL staining. (Panels b,b ′) Dorsal view of a stage 14 embryo (anterior toward left ) shows significantly increased cell death (black dots, arrow) on the neural crest region injected with Id3-MO (Red-Gal), compared with the uninjected side (arrowhead). (Panels c,c ′) Anterior view of a stage 17 embryo also shows elevated cell death (black dots, arrow) on the neural crest region injected with Id3-MO (X-gal staining, turquoise color), compared with the uninjected side (arrowhead). (Panels d,d ′) Anterior view of a stage 16 embryo injected with control-MO shows that cell death doesn't occur in the injected region (Red-Gal, arrow), compared with the uninjected side (arrowhead). The MO-injected region is magnified for each embryo in a ′- d ′. ( F ) The graph illustrating the results of TUNEL staining as percentages of TUNEL-negative (black bar) or TUNEL-positive (red bar) embryos out of the embryos injected with control-MO or Id3-MO.

    Article Snippet: The embryos injected with morpholino oligos were rehydrated in PBS, permeablized in PBS containing 0.5% Tween 20, and washed in TdT (terminal deoxynucletidyl transferase) buffer (Invitrogen).

    Techniques: Injection, In Situ Hybridization, Expressing, Marker, Proliferation Assay, Immunostaining, TUNEL Assay, Staining

    Id3 depletion leads to a loss of neural crest progenitors. ( A ) Morpholino antisense oligo sequence (Id3-MO) corresponding to a region 5′ upstream of the Id3 start codon (25 bp; -8 to -32) and its control oligo (control-MO) with seven mismatches (red letters). ( B ) Id3 protein synthesis was monitored in the absence of oligo (no MO) or in the presence of morpholino oligos (control, Id3 or Id2 oligo) in an in vitro translation system. Only Id3-MO specifically blocks Id3 protein translation. ( C ) Neurula-stage embryos injected with control-MO or Id3-MO. (Panel a ) slug expression (arrow) is intact on the side injected with control-MO (C-MO, Red-Gal). (Panels b - d ) Embryos injected with Id3-MO show the loss of early neural crest markers (arrows) on the injected side, which is marked by Red-Gal staining: slug (panel b ), sox10 (panel c ), and foxD3 (panel d ). ( D ) slug expression is not affected by Id2 depletion (arrow). ( E ) The loss of neural crest progenitors by Id3 depletion (panel a ) is rescued in the presence of chick Id3 (panels b,c ).

    Journal: Genes & Development

    Article Title: To proliferate or to die: role of Id3 in cell cycle progression and survival of neural crest progenitors

    doi: 10.1101/gad.1257405

    Figure Lengend Snippet: Id3 depletion leads to a loss of neural crest progenitors. ( A ) Morpholino antisense oligo sequence (Id3-MO) corresponding to a region 5′ upstream of the Id3 start codon (25 bp; -8 to -32) and its control oligo (control-MO) with seven mismatches (red letters). ( B ) Id3 protein synthesis was monitored in the absence of oligo (no MO) or in the presence of morpholino oligos (control, Id3 or Id2 oligo) in an in vitro translation system. Only Id3-MO specifically blocks Id3 protein translation. ( C ) Neurula-stage embryos injected with control-MO or Id3-MO. (Panel a ) slug expression (arrow) is intact on the side injected with control-MO (C-MO, Red-Gal). (Panels b - d ) Embryos injected with Id3-MO show the loss of early neural crest markers (arrows) on the injected side, which is marked by Red-Gal staining: slug (panel b ), sox10 (panel c ), and foxD3 (panel d ). ( D ) slug expression is not affected by Id2 depletion (arrow). ( E ) The loss of neural crest progenitors by Id3 depletion (panel a ) is rescued in the presence of chick Id3 (panels b,c ).

    Article Snippet: The embryos injected with morpholino oligos were rehydrated in PBS, permeablized in PBS containing 0.5% Tween 20, and washed in TdT (terminal deoxynucletidyl transferase) buffer (Invitrogen).

    Techniques: Sequencing, In Vitro, Injection, Expressing, Staining

    Inefficient splicing of MATa1 pre-mRNA, but not of other pre-mRNAs, in bud13Δ and ist3Δ mutants. (A) Schematic representations of the genes tested and the primers used. Exons and introns are shown as open boxes and lines, respectively. Open arrowheads, forward and reverse primers corresponding to exon sequences; closed arrowheads, alternative forward primers corresponding to ACT1 and RPS17A intron sequences (see Table S1 ). (B–D) Analyses of spliced and unspliced mRNAs using the exon-derived primers. Cells of diploid strains YEF473 (wild-type), STY254 ( bud13Δ/bud13Δ ), STY260 ( ist3Δ/ist3Δ ), and STY464 ( pml1Δ/pml1Δ ) were grown in YM-P medium at 30°C to OD 600 ≈0.5. Total RNA was prepared, treated with DNase, and reverse-transcribed into single-stranded cDNA using oligo (dT) 16 primers (see Materials and Methods ). cDNAs were then amplified by PCR using the appropriate primers. RNA samples that were not treated with DNase (DNase-) and/or not subjected to reverse transcription (RT−), as indicated, were used as controls. Molecular-size markers were run in the outside lanes in each gel; their sizes are indicated. (E) Analysis of unspliced ACT1 and RPS17A transcripts using the intron-derived forward primers. The reverse primers and other conditions were as described for B–D. The arrow indicates the expected size for the cDNA derived from unspliced pre-mRNA (approximately the same for each gene). The other bands in the ACT1 lanes appear to be nonspecific PCR products.

    Journal: PLoS ONE

    Article Title: Apparent Defect in Yeast Bud-Site Selection Due to a Specific Failure to Splice the Pre-mRNA of a Regulator of Cell-Type-Specific Transcription

    doi: 10.1371/journal.pone.0047621

    Figure Lengend Snippet: Inefficient splicing of MATa1 pre-mRNA, but not of other pre-mRNAs, in bud13Δ and ist3Δ mutants. (A) Schematic representations of the genes tested and the primers used. Exons and introns are shown as open boxes and lines, respectively. Open arrowheads, forward and reverse primers corresponding to exon sequences; closed arrowheads, alternative forward primers corresponding to ACT1 and RPS17A intron sequences (see Table S1 ). (B–D) Analyses of spliced and unspliced mRNAs using the exon-derived primers. Cells of diploid strains YEF473 (wild-type), STY254 ( bud13Δ/bud13Δ ), STY260 ( ist3Δ/ist3Δ ), and STY464 ( pml1Δ/pml1Δ ) were grown in YM-P medium at 30°C to OD 600 ≈0.5. Total RNA was prepared, treated with DNase, and reverse-transcribed into single-stranded cDNA using oligo (dT) 16 primers (see Materials and Methods ). cDNAs were then amplified by PCR using the appropriate primers. RNA samples that were not treated with DNase (DNase-) and/or not subjected to reverse transcription (RT−), as indicated, were used as controls. Molecular-size markers were run in the outside lanes in each gel; their sizes are indicated. (E) Analysis of unspliced ACT1 and RPS17A transcripts using the intron-derived forward primers. The reverse primers and other conditions were as described for B–D. The arrow indicates the expected size for the cDNA derived from unspliced pre-mRNA (approximately the same for each gene). The other bands in the ACT1 lanes appear to be nonspecific PCR products.

    Article Snippet: Single-stranded cDNA was synthesized using an oligo (dT)16 primer, MultiScribe™ reverse transcriptase (Applied Biosystems), and a regimen of 10 min at 25°C, 30 min at 48°C, and 5 min at 95°C, followed by storage at 4°C. cDNAs were then amplified by PCR using PrimeSTAR polymerase (Takara Bio) and gene-specific primers ( ).

    Techniques: Derivative Assay, Amplification, Polymerase Chain Reaction

    Measurements of influenza transcription. HIV-1-infected and -uninfected macrophages were infected with influenza A(H1N1)pdm09. After 24h, cells were lysed, RNA extracted and cDNA synthetized using oligo.dT and UNI-12 primers. Quantitative real time PCR was performed for the M1 gene. As a control, ribavirin-treated and -untreated influenza-infected macrophages were submitted to the same procedure described above (inset). * P

    Journal: PLoS ONE

    Article Title: HIV-1 and Its gp120 Inhibits the Influenza A(H1N1)pdm09 Life Cycle in an IFITM3-Dependent Fashion

    doi: 10.1371/journal.pone.0101056

    Figure Lengend Snippet: Measurements of influenza transcription. HIV-1-infected and -uninfected macrophages were infected with influenza A(H1N1)pdm09. After 24h, cells were lysed, RNA extracted and cDNA synthetized using oligo.dT and UNI-12 primers. Quantitative real time PCR was performed for the M1 gene. As a control, ribavirin-treated and -untreated influenza-infected macrophages were submitted to the same procedure described above (inset). * P

    Article Snippet: CDNA was synthetized with SuperScript III (Life Technologies) using oligo.dT (Life Technologies) or UNI-12 (5′-AGCRAAAGCAGG-3′) as the primers for first-strand synthesis, for 1 h at 45°C.

    Techniques: Infection, Real-time Polymerase Chain Reaction

    atp6v0b morpholinos do not phenocopy blowout

    Journal: Developmental biology

    Article Title: Zebrafish blowout provides genetic evidence for Patched1 mediated negative regulation of Hedgehog signaling within the proximal optic vesicle of the vertebrate eye

    doi: 10.1016/j.ydbio.2008.03.035

    Figure Lengend Snippet: atp6v0b morpholinos do not phenocopy blowout

    Article Snippet: ptc1 and atp6v0b morpholinos (MOs) were purchased from Open Biosystems (Huntsville, AL).

    Techniques: