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  • 96
    Millipore oligo
    Oligo, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 1184 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Qiagen oligo
    Oligo, supplied by Qiagen, used in various techniques. Bioz Stars score: 92/100, based on 868 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Beijing TransGen Biotech oligo
    Oligo, supplied by Beijing TransGen Biotech, used in various techniques. Bioz Stars score: 94/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Bio-Rad oligo
    Oligo, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 443 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Eurofins oligo
    Oligo, supplied by Eurofins, used in various techniques. Bioz Stars score: 96/100, based on 97 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    GE Healthcare oligo
    Oligo, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 93/100, based on 853 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Horizon Discovery oligo
    Oligo, supplied by Horizon Discovery, used in various techniques. Bioz Stars score: 92/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    iNtRON Biotechnology oligo
    Oligo, supplied by iNtRON Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 191 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Medprobe oligo
    Oligo, supplied by Medprobe, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    Promega oligo
    Oligo, supplied by Promega, used in various techniques. Bioz Stars score: 98/100, based on 6630 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    oligo  (Roche)
    96
    Roche oligo
    Oligo, supplied by Roche, used in various techniques. Bioz Stars score: 96/100, based on 1633 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    TaKaRa oligo
    Oligo, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1902 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Valiant oligo
    Oligo, supplied by Valiant, used in various techniques. Bioz Stars score: 92/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Bioneer Corporation oligo
    Oligo, supplied by Bioneer Corporation, used in various techniques. Bioz Stars score: 94/100, based on 215 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 215 article reviews
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    96
    Bioteke Corporation oligo
    Oligo, supplied by Bioteke Corporation, used in various techniques. Bioz Stars score: 96/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    EuroClone oligo
    Oligo, supplied by EuroClone, used in various techniques. Bioz Stars score: 94/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 38 article reviews
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    92
    IBA GmbH oligo
    Oligo, supplied by IBA GmbH, used in various techniques. Bioz Stars score: 92/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    PerkinElmer oligo
    Oligo, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 92/100, based on 185 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Ahram Biosystems oligo
    Oligo, supplied by Ahram Biosystems, used in various techniques. Bioz Stars score: 92/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Genset Corporation oligo
    Oligo, supplied by Genset Corporation, used in various techniques. Bioz Stars score: 92/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 35 article reviews
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    92
    MWG-Biotech oligo
    Oligo, supplied by MWG-Biotech, used in various techniques. Bioz Stars score: 92/100, based on 123 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Stratagene oligo
    Oligo, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 318 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Supertechs oligo
    Oligo, supplied by Supertechs, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    tiangen biotech co oligo
    Oligo, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 93/100, based on 196 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 196 article reviews
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    96
    Toyobo oligo
    Oligo, supplied by Toyobo, used in various techniques. Bioz Stars score: 96/100, based on 547 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Macrogen oligo
    Oligo, supplied by Macrogen, used in various techniques. Bioz Stars score: 92/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Microsynth oligo
    Oligo, supplied by Microsynth, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher oligo
    Oligo, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 24851 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Beijing Solarbio Science oligo
    Oligo, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 92/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Bioo Scientific oligoes
    Oligoes, supplied by Bioo Scientific, used in various techniques. Bioz Stars score: 79/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Zymo Research oligo clean
    In vivo production of ssDNA. ( a ) Schematic for the conversion of the <t>r_oligo</t> gene to ssDNA. The r_oligo gene contains both the desired ssDNA sequence and the HTBS part, which serves as a terminator (black, ssDNA sequence; orange, A/U region; brown, PBS; purple, hairpin). ( b ) The impact of different combinations of RT expression on ssDNA production is shown. Data are shown for the production of a 205-nt ssDNA (r_oligo_205) under purification conditions preventing the removal of the HTBS (RNAse A+150 mM NaCl). The red triangle shows the predicted location of the band (note that the ladder is based on double stranded DNA). The bands are from the same gel and the image processed once, but the order changed for publication. For the full gel in its original order, see Supplementary Fig. 1 . The ssDNA sequence and reverse transcriptase sequences are added in Supplementary Tables 2 and 3 . ( c ) The expression of ssDNA after 18 h growth in the presence (+IPTG, 1 mM) and absence of IPTG (−IPTG) under conditions preserving the HTBS. To confirm that the band is ssDNA, the same sample is exposed to DNase (+IPTG/+DNase, 1 mM/4 units). ( d ) For the same system as in c , the ssDNA is treated to remove the HTBS <t>RNA</t> (RNAse and no salt). ( e ) Comparison of an in vivo produced ssDNA with commercial chemically synthesized ssDNAs. The ladder was calculated using commercial oligos of defined size run simultaneously in the gel. The ssDNA sequence is in Supplementary Table 2 . ( f ) Sequencing analysis of the in vivo produced 72-mer. The ‘prediction' is the complementary sequence of the expected ssDNA (Methods).
    Oligo Clean, supplied by Zymo Research, used in various techniques. Bioz Stars score: 91/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Thermo Fisher ultrahybtm oligo
    In vivo production of ssDNA. ( a ) Schematic for the conversion of the <t>r_oligo</t> gene to ssDNA. The r_oligo gene contains both the desired ssDNA sequence and the HTBS part, which serves as a terminator (black, ssDNA sequence; orange, A/U region; brown, PBS; purple, hairpin). ( b ) The impact of different combinations of RT expression on ssDNA production is shown. Data are shown for the production of a 205-nt ssDNA (r_oligo_205) under purification conditions preventing the removal of the HTBS (RNAse A+150 mM NaCl). The red triangle shows the predicted location of the band (note that the ladder is based on double stranded DNA). The bands are from the same gel and the image processed once, but the order changed for publication. For the full gel in its original order, see Supplementary Fig. 1 . The ssDNA sequence and reverse transcriptase sequences are added in Supplementary Tables 2 and 3 . ( c ) The expression of ssDNA after 18 h growth in the presence (+IPTG, 1 mM) and absence of IPTG (−IPTG) under conditions preserving the HTBS. To confirm that the band is ssDNA, the same sample is exposed to DNase (+IPTG/+DNase, 1 mM/4 units). ( d ) For the same system as in c , the ssDNA is treated to remove the HTBS <t>RNA</t> (RNAse and no salt). ( e ) Comparison of an in vivo produced ssDNA with commercial chemically synthesized ssDNAs. The ladder was calculated using commercial oligos of defined size run simultaneously in the gel. The ssDNA sequence is in Supplementary Table 2 . ( f ) Sequencing analysis of the in vivo produced 72-mer. The ‘prediction' is the complementary sequence of the expected ssDNA (Methods).
    Ultrahybtm Oligo, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultrahybtm oligo/product/Thermo Fisher
    Average 79 stars, based on 9 article reviews
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    Image Search Results


    In vivo production of ssDNA. ( a ) Schematic for the conversion of the r_oligo gene to ssDNA. The r_oligo gene contains both the desired ssDNA sequence and the HTBS part, which serves as a terminator (black, ssDNA sequence; orange, A/U region; brown, PBS; purple, hairpin). ( b ) The impact of different combinations of RT expression on ssDNA production is shown. Data are shown for the production of a 205-nt ssDNA (r_oligo_205) under purification conditions preventing the removal of the HTBS (RNAse A+150 mM NaCl). The red triangle shows the predicted location of the band (note that the ladder is based on double stranded DNA). The bands are from the same gel and the image processed once, but the order changed for publication. For the full gel in its original order, see Supplementary Fig. 1 . The ssDNA sequence and reverse transcriptase sequences are added in Supplementary Tables 2 and 3 . ( c ) The expression of ssDNA after 18 h growth in the presence (+IPTG, 1 mM) and absence of IPTG (−IPTG) under conditions preserving the HTBS. To confirm that the band is ssDNA, the same sample is exposed to DNase (+IPTG/+DNase, 1 mM/4 units). ( d ) For the same system as in c , the ssDNA is treated to remove the HTBS RNA (RNAse and no salt). ( e ) Comparison of an in vivo produced ssDNA with commercial chemically synthesized ssDNAs. The ladder was calculated using commercial oligos of defined size run simultaneously in the gel. The ssDNA sequence is in Supplementary Table 2 . ( f ) Sequencing analysis of the in vivo produced 72-mer. The ‘prediction' is the complementary sequence of the expected ssDNA (Methods).

    Journal: Nature Communications

    Article Title: Genetic encoding of DNA nanostructures and their self-assembly in living bacteria

    doi: 10.1038/ncomms11179

    Figure Lengend Snippet: In vivo production of ssDNA. ( a ) Schematic for the conversion of the r_oligo gene to ssDNA. The r_oligo gene contains both the desired ssDNA sequence and the HTBS part, which serves as a terminator (black, ssDNA sequence; orange, A/U region; brown, PBS; purple, hairpin). ( b ) The impact of different combinations of RT expression on ssDNA production is shown. Data are shown for the production of a 205-nt ssDNA (r_oligo_205) under purification conditions preventing the removal of the HTBS (RNAse A+150 mM NaCl). The red triangle shows the predicted location of the band (note that the ladder is based on double stranded DNA). The bands are from the same gel and the image processed once, but the order changed for publication. For the full gel in its original order, see Supplementary Fig. 1 . The ssDNA sequence and reverse transcriptase sequences are added in Supplementary Tables 2 and 3 . ( c ) The expression of ssDNA after 18 h growth in the presence (+IPTG, 1 mM) and absence of IPTG (−IPTG) under conditions preserving the HTBS. To confirm that the band is ssDNA, the same sample is exposed to DNase (+IPTG/+DNase, 1 mM/4 units). ( d ) For the same system as in c , the ssDNA is treated to remove the HTBS RNA (RNAse and no salt). ( e ) Comparison of an in vivo produced ssDNA with commercial chemically synthesized ssDNAs. The ladder was calculated using commercial oligos of defined size run simultaneously in the gel. The ssDNA sequence is in Supplementary Table 2 . ( f ) Sequencing analysis of the in vivo produced 72-mer. The ‘prediction' is the complementary sequence of the expected ssDNA (Methods).

    Article Snippet: After RNA degradation, the solutions were cleaned and concentrated using oligo clean and concentrator kit (ZYMO Research Corp., D4061 ZYMO).

    Techniques: In Vivo, Sequencing, Expressing, Purification, Preserving, Produced, Synthesized