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  • 96
    BioVendor Instruments elisa kit
    Elisa Kit, supplied by BioVendor Instruments, used in various techniques. Bioz Stars score: 96/100, based on 578 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore 4 oh tamoxifen
    4 Oh Tamoxifen, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1000 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Waters Corporation oh d3
    Oh D3, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 92/100, based on 169 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    DiaSorin liaison 25 oh vitamin d total assay
    ADVIA Centaur® <t>Vitamin</t> D <t>Total</t>
    Liaison 25 Oh Vitamin D Total Assay, supplied by DiaSorin, used in various techniques. Bioz Stars score: 90/100, based on 205 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Tocris 8 oh dpat
    5-HT, 5-Htr1a specific agonist and 5-Htr1b specific agonist inhibits cell proliferation in BPH-1 and WPMY-1 human prostatic cells without any effect in PNT1A cells. ( a , b , c ) Effect of 5-HT, ( d , e , f ) 5-Htr1a specific agonist, <t>8-OH-DPAT,</t> and ( g , h , i ) 5-Htr1b specific agonist, Anpirtoline, on cell proliferation of BPH-1, PNT1A and WPMY-1 cells as quantified by Ki-67 positive cells/total cell number ratio. The data are expressed relative to control condition (0 µM 5-HT without testosterone supplementation) and were reproduced in at least three independent experiments. Error bars indicate s.e.m. n.s . non-significant; 5-HT, serotonin; * p
    8 Oh Dpat, supplied by Tocris, used in various techniques. Bioz Stars score: 99/100, based on 118 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore l cysteine
    5-HT, 5-Htr1a specific agonist and 5-Htr1b specific agonist inhibits cell proliferation in BPH-1 and WPMY-1 human prostatic cells without any effect in PNT1A cells. ( a , b , c ) Effect of 5-HT, ( d , e , f ) 5-Htr1a specific agonist, <t>8-OH-DPAT,</t> and ( g , h , i ) 5-Htr1b specific agonist, Anpirtoline, on cell proliferation of BPH-1, PNT1A and WPMY-1 cells as quantified by Ki-67 positive cells/total cell number ratio. The data are expressed relative to control condition (0 µM 5-HT without testosterone supplementation) and were reproduced in at least three independent experiments. Error bars indicate s.e.m. n.s . non-significant; 5-HT, serotonin; * p
    L Cysteine, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3331 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Cayman Chemical 1 25 oh 2 d3
    5-HT, 5-Htr1a specific agonist and 5-Htr1b specific agonist inhibits cell proliferation in BPH-1 and WPMY-1 human prostatic cells without any effect in PNT1A cells. ( a , b , c ) Effect of 5-HT, ( d , e , f ) 5-Htr1a specific agonist, <t>8-OH-DPAT,</t> and ( g , h , i ) 5-Htr1b specific agonist, Anpirtoline, on cell proliferation of BPH-1, PNT1A and WPMY-1 cells as quantified by Ki-67 positive cells/total cell number ratio. The data are expressed relative to control condition (0 µM 5-HT without testosterone supplementation) and were reproduced in at least three independent experiments. Error bars indicate s.e.m. n.s . non-significant; 5-HT, serotonin; * p
    1 25 Oh 2 D3, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    Millipore fmoc lys boc oh
    5-HT, 5-Htr1a specific agonist and 5-Htr1b specific agonist inhibits cell proliferation in BPH-1 and WPMY-1 human prostatic cells without any effect in PNT1A cells. ( a , b , c ) Effect of 5-HT, ( d , e , f ) 5-Htr1a specific agonist, <t>8-OH-DPAT,</t> and ( g , h , i ) 5-Htr1b specific agonist, Anpirtoline, on cell proliferation of BPH-1, PNT1A and WPMY-1 cells as quantified by Ki-67 positive cells/total cell number ratio. The data are expressed relative to control condition (0 µM 5-HT without testosterone supplementation) and were reproduced in at least three independent experiments. Error bars indicate s.e.m. n.s . non-significant; 5-HT, serotonin; * p
    Fmoc Lys Boc Oh, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    DiaSorin oh d3
    5-HT, 5-Htr1a specific agonist and 5-Htr1b specific agonist inhibits cell proliferation in BPH-1 and WPMY-1 human prostatic cells without any effect in PNT1A cells. ( a , b , c ) Effect of 5-HT, ( d , e , f ) 5-Htr1a specific agonist, <t>8-OH-DPAT,</t> and ( g , h , i ) 5-Htr1b specific agonist, Anpirtoline, on cell proliferation of BPH-1, PNT1A and WPMY-1 cells as quantified by Ki-67 positive cells/total cell number ratio. The data are expressed relative to control condition (0 µM 5-HT without testosterone supplementation) and were reproduced in at least three independent experiments. Error bars indicate s.e.m. n.s . non-significant; 5-HT, serotonin; * p
    Oh D3, supplied by DiaSorin, used in various techniques. Bioz Stars score: 92/100, based on 100 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    DiaSorin 25 oh vitamin d
    Associations between serum total 25‐ OH <t>vitamin</t> D quintile and mortality. Median 25‐ OH vitamin D is shown for each quintile. Demographic risk factors were age and sex. Clinical risk factors were body mass index, smoking status (current, prior vs never), renal disease, hypertension, hyperlipidemia, type 2 diabetes mellitus, family history of heart disease, prior myocardial infarction, congestive heart failure, whether ejection fraction was measured, ejection fraction (
    25 Oh Vitamin D, supplied by DiaSorin, used in various techniques. Bioz Stars score: 90/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore fmoc gly oh
    Associations between serum total 25‐ OH <t>vitamin</t> D quintile and mortality. Median 25‐ OH vitamin D is shown for each quintile. Demographic risk factors were age and sex. Clinical risk factors were body mass index, smoking status (current, prior vs never), renal disease, hypertension, hyperlipidemia, type 2 diabetes mellitus, family history of heart disease, prior myocardial infarction, congestive heart failure, whether ejection fraction was measured, ejection fraction (
    Fmoc Gly Oh, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 86 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Fisher Scientific nh4 oh
    Associations between serum total 25‐ OH <t>vitamin</t> D quintile and mortality. Median 25‐ OH vitamin D is shown for each quintile. Demographic risk factors were age and sex. Clinical risk factors were body mass index, smoking status (current, prior vs never), renal disease, hypertension, hyperlipidemia, type 2 diabetes mellitus, family history of heart disease, prior myocardial infarction, congestive heart failure, whether ejection fraction was measured, ejection fraction (
    Nh4 Oh, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    DSM NV oh d3
    Associations between serum total 25‐ OH <t>vitamin</t> D quintile and mortality. Median 25‐ OH vitamin D is shown for each quintile. Demographic risk factors were age and sex. Clinical risk factors were body mass index, smoking status (current, prior vs never), renal disease, hypertension, hyperlipidemia, type 2 diabetes mellitus, family history of heart disease, prior myocardial infarction, congestive heart failure, whether ejection fraction was measured, ejection fraction (
    Oh D3, supplied by DSM NV, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore fmoc arg pbf oh
    Associations between serum total 25‐ OH <t>vitamin</t> D quintile and mortality. Median 25‐ OH vitamin D is shown for each quintile. Demographic risk factors were age and sex. Clinical risk factors were body mass index, smoking status (current, prior vs never), renal disease, hypertension, hyperlipidemia, type 2 diabetes mellitus, family history of heart disease, prior myocardial infarction, congestive heart failure, whether ejection fraction was measured, ejection fraction (
    Fmoc Arg Pbf Oh, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Agilent technologies oh d3
    Associations between serum total 25‐ OH <t>vitamin</t> D quintile and mortality. Median 25‐ OH vitamin D is shown for each quintile. Demographic risk factors were age and sex. Clinical risk factors were body mass index, smoking status (current, prior vs never), renal disease, hypertension, hyperlipidemia, type 2 diabetes mellitus, family history of heart disease, prior myocardial infarction, congestive heart failure, whether ejection fraction was measured, ejection fraction (
    Oh D3, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 64 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Merck & Co nh4 oh
    Associations between serum total 25‐ OH <t>vitamin</t> D quintile and mortality. Median 25‐ OH vitamin D is shown for each quintile. Demographic risk factors were age and sex. Clinical risk factors were body mass index, smoking status (current, prior vs never), renal disease, hypertension, hyperlipidemia, type 2 diabetes mellitus, family history of heart disease, prior myocardial infarction, congestive heart failure, whether ejection fraction was measured, ejection fraction (
    Nh4 Oh, supplied by Merck & Co, used in various techniques. Bioz Stars score: 92/100, based on 77 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore nh4 oh
    Associations between serum total 25‐ OH <t>vitamin</t> D quintile and mortality. Median 25‐ OH vitamin D is shown for each quintile. Demographic risk factors were age and sex. Clinical risk factors were body mass index, smoking status (current, prior vs never), renal disease, hypertension, hyperlipidemia, type 2 diabetes mellitus, family history of heart disease, prior myocardial infarction, congestive heart failure, whether ejection fraction was measured, ejection fraction (
    Nh4 Oh, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 570 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Cambridge Isotope Laboratories nh2 oh
    Associations between serum total 25‐ OH <t>vitamin</t> D quintile and mortality. Median 25‐ OH vitamin D is shown for each quintile. Demographic risk factors were age and sex. Clinical risk factors were body mass index, smoking status (current, prior vs never), renal disease, hypertension, hyperlipidemia, type 2 diabetes mellitus, family history of heart disease, prior myocardial infarction, congestive heart failure, whether ejection fraction was measured, ejection fraction (
    Nh2 Oh, supplied by Cambridge Isotope Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore fmoc cys trt oh
    Associations between serum total 25‐ OH <t>vitamin</t> D quintile and mortality. Median 25‐ OH vitamin D is shown for each quintile. Demographic risk factors were age and sex. Clinical risk factors were body mass index, smoking status (current, prior vs never), renal disease, hypertension, hyperlipidemia, type 2 diabetes mellitus, family history of heart disease, prior myocardial infarction, congestive heart failure, whether ejection fraction was measured, ejection fraction (
    Fmoc Cys Trt Oh, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Merck & Co chcl3 ch3 oh
    Associations between serum total 25‐ OH <t>vitamin</t> D quintile and mortality. Median 25‐ OH vitamin D is shown for each quintile. Demographic risk factors were age and sex. Clinical risk factors were body mass index, smoking status (current, prior vs never), renal disease, hypertension, hyperlipidemia, type 2 diabetes mellitus, family history of heart disease, prior myocardial infarction, congestive heart failure, whether ejection fraction was measured, ejection fraction (
    Chcl3 Ch3 Oh, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Diasource free 25 oh d
    Associations between serum total 25‐ OH <t>vitamin</t> D quintile and mortality. Median 25‐ OH vitamin D is shown for each quintile. Demographic risk factors were age and sex. Clinical risk factors were body mass index, smoking status (current, prior vs never), renal disease, hypertension, hyperlipidemia, type 2 diabetes mellitus, family history of heart disease, prior myocardial infarction, congestive heart failure, whether ejection fraction was measured, ejection fraction (
    Free 25 Oh D, supplied by Diasource, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Johnson & Johnson oh jj
    Associations between serum total 25‐ OH <t>vitamin</t> D quintile and mortality. Median 25‐ OH vitamin D is shown for each quintile. Demographic risk factors were age and sex. Clinical risk factors were body mass index, smoking status (current, prior vs never), renal disease, hypertension, hyperlipidemia, type 2 diabetes mellitus, family history of heart disease, prior myocardial infarction, congestive heart failure, whether ejection fraction was measured, ejection fraction (
    Oh Jj, supplied by Johnson & Johnson, used in various techniques. Bioz Stars score: 88/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Tanabe ryu oh
    Associations between serum total 25‐ OH <t>vitamin</t> D quintile and mortality. Median 25‐ OH vitamin D is shown for each quintile. Demographic risk factors were age and sex. Clinical risk factors were body mass index, smoking status (current, prior vs never), renal disease, hypertension, hyperlipidemia, type 2 diabetes mellitus, family history of heart disease, prior myocardial infarction, congestive heart failure, whether ejection fraction was measured, ejection fraction (
    Ryu Oh, supplied by Tanabe, used in various techniques. Bioz Stars score: 90/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore fmoc ala oh
    Associations between serum total 25‐ OH <t>vitamin</t> D quintile and mortality. Median 25‐ OH vitamin D is shown for each quintile. Demographic risk factors were age and sex. Clinical risk factors were body mass index, smoking status (current, prior vs never), renal disease, hypertension, hyperlipidemia, type 2 diabetes mellitus, family history of heart disease, prior myocardial infarction, congestive heart failure, whether ejection fraction was measured, ejection fraction (
    Fmoc Ala Oh, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Tri γ glutamic acid
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    Image Search Results


    ADVIA Centaur® Vitamin D Total

    Journal: Clinical biochemistry

    Article Title: Performance Evaluation of Siemens ADVIA Centaur and Roche MODULAR Analytics E170 Total 25-OH Vitamin D Assays

    doi: 10.1016/j.clinbiochem.2012.06.002

    Figure Lengend Snippet: ADVIA Centaur® Vitamin D Total

    Article Snippet: The DiaSorin LIAISON 25 OH Vitamin D TOTAL assay is an automated direct competitive chemiluminescent immunoassay that detects 25-OH vitamins D2 and D3 and other hydroxylated metabolites in serum or plasma.

    Techniques:

    Linearity of the ADVIA Centaur and Modular E170 total Vitamin D assays.

    Journal: Clinical biochemistry

    Article Title: Performance Evaluation of Siemens ADVIA Centaur and Roche MODULAR Analytics E170 Total 25-OH Vitamin D Assays

    doi: 10.1016/j.clinbiochem.2012.06.002

    Figure Lengend Snippet: Linearity of the ADVIA Centaur and Modular E170 total Vitamin D assays.

    Article Snippet: The DiaSorin LIAISON 25 OH Vitamin D TOTAL assay is an automated direct competitive chemiluminescent immunoassay that detects 25-OH vitamins D2 and D3 and other hydroxylated metabolites in serum or plasma.

    Techniques:

    5-HT, 5-Htr1a specific agonist and 5-Htr1b specific agonist inhibits cell proliferation in BPH-1 and WPMY-1 human prostatic cells without any effect in PNT1A cells. ( a , b , c ) Effect of 5-HT, ( d , e , f ) 5-Htr1a specific agonist, 8-OH-DPAT, and ( g , h , i ) 5-Htr1b specific agonist, Anpirtoline, on cell proliferation of BPH-1, PNT1A and WPMY-1 cells as quantified by Ki-67 positive cells/total cell number ratio. The data are expressed relative to control condition (0 µM 5-HT without testosterone supplementation) and were reproduced in at least three independent experiments. Error bars indicate s.e.m. n.s . non-significant; 5-HT, serotonin; * p

    Journal: Scientific Reports

    Article Title: Serotonin regulates prostate growth through androgen receptor modulation

    doi: 10.1038/s41598-017-15832-5

    Figure Lengend Snippet: 5-HT, 5-Htr1a specific agonist and 5-Htr1b specific agonist inhibits cell proliferation in BPH-1 and WPMY-1 human prostatic cells without any effect in PNT1A cells. ( a , b , c ) Effect of 5-HT, ( d , e , f ) 5-Htr1a specific agonist, 8-OH-DPAT, and ( g , h , i ) 5-Htr1b specific agonist, Anpirtoline, on cell proliferation of BPH-1, PNT1A and WPMY-1 cells as quantified by Ki-67 positive cells/total cell number ratio. The data are expressed relative to control condition (0 µM 5-HT without testosterone supplementation) and were reproduced in at least three independent experiments. Error bars indicate s.e.m. n.s . non-significant; 5-HT, serotonin; * p

    Article Snippet: The 5-Htr1a specific agonist, 8-OH-DPAT and the 5-Htr1b specific agonist, Anpirtoline, were purchased from Tocris-Bioscience (Bristol, UK).

    Techniques:

    5-HT, 5-Htr1a specific agonist and 5-Htr1b specific agonist down-regulates AR expression in rat ventral prostate. ( a ) Western blot analysis of AR expression in prostate explants treated with increasing doses of 5-HT, specific 5-Htr1a agonist, 8-OH-DPAT, and specific 5-Htr1b agonist, anpirtoline. ( b ) Quantification of AR protein in VPs treated with different concentrations of 5-HT in medium conditions without or with testosterone supplementation, n ≥ 3 (each sample contained a pool of 4 VPs). ( c ) Quantification of AR protein in VPs treated with different concentrations of 8-OH-DPAT in medium conditions without or with testosterone supplementation, n ≥ 3 (each sample contained a pool of 4 VPs). ( d ) Quantification of AR protein in VPs treated with different concentrations of Anpirtoline in medium conditions without or with testosterone supplementation, n ≥ 3 (each sample contained a pool of 4 VPs). Error bars indicate s.e.m. n.s . non-significant; * p

    Journal: Scientific Reports

    Article Title: Serotonin regulates prostate growth through androgen receptor modulation

    doi: 10.1038/s41598-017-15832-5

    Figure Lengend Snippet: 5-HT, 5-Htr1a specific agonist and 5-Htr1b specific agonist down-regulates AR expression in rat ventral prostate. ( a ) Western blot analysis of AR expression in prostate explants treated with increasing doses of 5-HT, specific 5-Htr1a agonist, 8-OH-DPAT, and specific 5-Htr1b agonist, anpirtoline. ( b ) Quantification of AR protein in VPs treated with different concentrations of 5-HT in medium conditions without or with testosterone supplementation, n ≥ 3 (each sample contained a pool of 4 VPs). ( c ) Quantification of AR protein in VPs treated with different concentrations of 8-OH-DPAT in medium conditions without or with testosterone supplementation, n ≥ 3 (each sample contained a pool of 4 VPs). ( d ) Quantification of AR protein in VPs treated with different concentrations of Anpirtoline in medium conditions without or with testosterone supplementation, n ≥ 3 (each sample contained a pool of 4 VPs). Error bars indicate s.e.m. n.s . non-significant; * p

    Article Snippet: The 5-Htr1a specific agonist, 8-OH-DPAT and the 5-Htr1b specific agonist, Anpirtoline, were purchased from Tocris-Bioscience (Bristol, UK).

    Techniques: Expressing, Western Blot

    5-HT, 5-Htr1a specific agonist and 5-Htr1b specific agonist inhibits cell viability in BPH-1 and WPMY-1 human prostatic cells without any effect in PNT1A cells. ( a , b , c ) Effect of 5-HT on cell viability analyzed by MTS assay in BPH-1, PNT1A and WPMY-1 cells. ( d ) Immunofluorescence analysis of 5-Htr1a and 5-Htr1b expression in BPH-1, PNT1A and WPMY-1 cells. ( e , f , g ) Effect of 5-Htr1a specific agonist, 8-OH-DPAT, and ( h , i , j ) 5-Htr1b specific agonist, Anpirtoline, on cell viability analyzed by MTS assay in BPH-1, PNT1A and WPMY-1 cells. The data are expressed relative to control condition (0 µM 5-HT without testosterone supplementation) and were reproduced in at least three independent experiments. Error bars indicate s.e.m. n.s . non-significant; 5-HT, serotonin; * p

    Journal: Scientific Reports

    Article Title: Serotonin regulates prostate growth through androgen receptor modulation

    doi: 10.1038/s41598-017-15832-5

    Figure Lengend Snippet: 5-HT, 5-Htr1a specific agonist and 5-Htr1b specific agonist inhibits cell viability in BPH-1 and WPMY-1 human prostatic cells without any effect in PNT1A cells. ( a , b , c ) Effect of 5-HT on cell viability analyzed by MTS assay in BPH-1, PNT1A and WPMY-1 cells. ( d ) Immunofluorescence analysis of 5-Htr1a and 5-Htr1b expression in BPH-1, PNT1A and WPMY-1 cells. ( e , f , g ) Effect of 5-Htr1a specific agonist, 8-OH-DPAT, and ( h , i , j ) 5-Htr1b specific agonist, Anpirtoline, on cell viability analyzed by MTS assay in BPH-1, PNT1A and WPMY-1 cells. The data are expressed relative to control condition (0 µM 5-HT without testosterone supplementation) and were reproduced in at least three independent experiments. Error bars indicate s.e.m. n.s . non-significant; 5-HT, serotonin; * p

    Article Snippet: The 5-Htr1a specific agonist, 8-OH-DPAT and the 5-Htr1b specific agonist, Anpirtoline, were purchased from Tocris-Bioscience (Bristol, UK).

    Techniques: MTS Assay, Immunofluorescence, Expressing

    5-HT, 5-Htr1a specific agonist and 5-Htr1b specific agonist down-regulates AR expression in human prostatic cells. ( a ) Western blot analysis of AR expression in the three cell lines after 5-HT, 8-OH-DPAT and anpirtoline treatment. ( b ) Quantification of AR in BPH-1 and ( c ) WPMY-1 cells after 5-HT treatment in medium conditions without or with Testosterone supplementation. ( d ) Quantification of AR protein levels in BPH-1 and ( e ) WPMY-1 cells after 8-OH-DPAT treatment in medium conditions without or with Testosterone supplementation. ( f ) Quantification of AR protein levels in BPH-1 and ( g ) WPMY-1 cells after Anpirtoline treatment in medium conditions without or with Testosterone supplementation. The data are expressed relative to control condition (0 µM 5-HT without testosterone supplementation) and were reproduced in at least three independent experiments. Full, uncropped gel images are shown. Error bars indicate s.e.m. n.s . non-significant; * P

    Journal: Scientific Reports

    Article Title: Serotonin regulates prostate growth through androgen receptor modulation

    doi: 10.1038/s41598-017-15832-5

    Figure Lengend Snippet: 5-HT, 5-Htr1a specific agonist and 5-Htr1b specific agonist down-regulates AR expression in human prostatic cells. ( a ) Western blot analysis of AR expression in the three cell lines after 5-HT, 8-OH-DPAT and anpirtoline treatment. ( b ) Quantification of AR in BPH-1 and ( c ) WPMY-1 cells after 5-HT treatment in medium conditions without or with Testosterone supplementation. ( d ) Quantification of AR protein levels in BPH-1 and ( e ) WPMY-1 cells after 8-OH-DPAT treatment in medium conditions without or with Testosterone supplementation. ( f ) Quantification of AR protein levels in BPH-1 and ( g ) WPMY-1 cells after Anpirtoline treatment in medium conditions without or with Testosterone supplementation. The data are expressed relative to control condition (0 µM 5-HT without testosterone supplementation) and were reproduced in at least three independent experiments. Full, uncropped gel images are shown. Error bars indicate s.e.m. n.s . non-significant; * P

    Article Snippet: The 5-Htr1a specific agonist, 8-OH-DPAT and the 5-Htr1b specific agonist, Anpirtoline, were purchased from Tocris-Bioscience (Bristol, UK).

    Techniques: Expressing, Western Blot

    5-HT, 5-Htr1a specific agonist and 5-Htr1b specific agonist inhibit prostate branching morphogenesis. ( a ) Photographs of representative VPs at D 0 and at D 4 of culture treated with different 5-HT concentrations. ( b ) Morphometric analysis of the effect of 5-HT on VPs area and ( c ) number of peripheral buds ( n ≥ 12 VPs per group). ( d ) Immunofluorescence analysis of 5-Htr1a and ( e ) 5-Htr1b expression in the rat prostate. ( f ) Photographs of representative VPs at D0 and at D4 of culture treated with different 8-OH-DPAT concentrations. ( g ) Morphometric analysis of the effect of 8-OH-DPAT on VPs area and ( h ) number of peripheral buds ( n ≥ 12 VPs per group). ( i ) Photographs of representative VPs at D0 and at D4 of culture treated with different anpirtoline concentrations. ( j ) Morphometric analysis of the effect of Anpirtoline on VPs area and ( k ) number of peripheral buds ( n ≥ 12 VPs per group). Error bars indicate s.e.m. *** p

    Journal: Scientific Reports

    Article Title: Serotonin regulates prostate growth through androgen receptor modulation

    doi: 10.1038/s41598-017-15832-5

    Figure Lengend Snippet: 5-HT, 5-Htr1a specific agonist and 5-Htr1b specific agonist inhibit prostate branching morphogenesis. ( a ) Photographs of representative VPs at D 0 and at D 4 of culture treated with different 5-HT concentrations. ( b ) Morphometric analysis of the effect of 5-HT on VPs area and ( c ) number of peripheral buds ( n ≥ 12 VPs per group). ( d ) Immunofluorescence analysis of 5-Htr1a and ( e ) 5-Htr1b expression in the rat prostate. ( f ) Photographs of representative VPs at D0 and at D4 of culture treated with different 8-OH-DPAT concentrations. ( g ) Morphometric analysis of the effect of 8-OH-DPAT on VPs area and ( h ) number of peripheral buds ( n ≥ 12 VPs per group). ( i ) Photographs of representative VPs at D0 and at D4 of culture treated with different anpirtoline concentrations. ( j ) Morphometric analysis of the effect of Anpirtoline on VPs area and ( k ) number of peripheral buds ( n ≥ 12 VPs per group). Error bars indicate s.e.m. *** p

    Article Snippet: The 5-Htr1a specific agonist, 8-OH-DPAT and the 5-Htr1b specific agonist, Anpirtoline, were purchased from Tocris-Bioscience (Bristol, UK).

    Techniques: Immunofluorescence, Expressing

    Associations between serum total 25‐ OH vitamin D quintile and mortality. Median 25‐ OH vitamin D is shown for each quintile. Demographic risk factors were age and sex. Clinical risk factors were body mass index, smoking status (current, prior vs never), renal disease, hypertension, hyperlipidemia, type 2 diabetes mellitus, family history of heart disease, prior myocardial infarction, congestive heart failure, whether ejection fraction was measured, ejection fraction (

    Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

    Article Title: Serum Total 25‐OH Vitamin D Adds Little Prognostic Value in Patients Undergoing Coronary Catheterization

    doi: 10.1161/JAHA.116.004289

    Figure Lengend Snippet: Associations between serum total 25‐ OH vitamin D quintile and mortality. Median 25‐ OH vitamin D is shown for each quintile. Demographic risk factors were age and sex. Clinical risk factors were body mass index, smoking status (current, prior vs never), renal disease, hypertension, hyperlipidemia, type 2 diabetes mellitus, family history of heart disease, prior myocardial infarction, congestive heart failure, whether ejection fraction was measured, ejection fraction (

    Article Snippet: Because 25‐OH vitamin D was measured by liquid chromatography–tandem mass spectrometry in Edmonton and by immunoassay (Liaison; DiaSorin) in Calgary, 240 identical samples were run by each method (R 2 =0.8, slope=1.0, intercept=−8), and Edmonton results were corrected to match those from Calgary by subtracting 8 nmol/L.

    Techniques: