Journal: Clinical biochemistry

Article Title: Performance Evaluation of Siemens ADVIA Centaur and Roche MODULAR Analytics E170 Total 25-OH Vitamin D Assays

doi: 10.1016/j.clinbiochem.2012.06.002

Figure Lengend Snippet: ADVIA Centaur® Vitamin D Total

Article Snippet: The DiaSorin LIAISON 25 OH Vitamin D TOTAL assay is an automated direct competitive chemiluminescent immunoassay that detects 25-OH vitamins D2 and D3 and other hydroxylated metabolites in serum or plasma.

Techniques:

Journal: Clinical biochemistry

Article Title: Performance Evaluation of Siemens ADVIA Centaur and Roche MODULAR Analytics E170 Total 25-OH Vitamin D Assays

doi: 10.1016/j.clinbiochem.2012.06.002

Figure Lengend Snippet: Linearity of the ADVIA Centaur and Modular E170 total Vitamin D assays.

Article Snippet: The DiaSorin LIAISON 25 OH Vitamin D TOTAL assay is an automated direct competitive chemiluminescent immunoassay that detects 25-OH vitamins D2 and D3 and other hydroxylated metabolites in serum or plasma.

Techniques:

Journal: Scientific Reports

Article Title: Serotonin regulates prostate growth through androgen receptor modulation

doi: 10.1038/s41598-017-15832-5

Figure Lengend Snippet: 5-HT, 5-Htr1a specific agonist and 5-Htr1b specific agonist inhibits cell proliferation in BPH-1 and WPMY-1 human prostatic cells without any effect in PNT1A cells. ( a , b , c ) Effect of 5-HT, ( d , e , f ) 5-Htr1a specific agonist, 8-OH-DPAT, and ( g , h , i ) 5-Htr1b specific agonist, Anpirtoline, on cell proliferation of BPH-1, PNT1A and WPMY-1 cells as quantified by Ki-67 positive cells/total cell number ratio. The data are expressed relative to control condition (0 µM 5-HT without testosterone supplementation) and were reproduced in at least three independent experiments. Error bars indicate s.e.m. n.s . non-significant; 5-HT, serotonin; * p

Article Snippet: The 5-Htr1a specific agonist, 8-OH-DPAT and the 5-Htr1b specific agonist, Anpirtoline, were purchased from Tocris-Bioscience (Bristol, UK).

Techniques:

Journal: Scientific Reports

Article Title: Serotonin regulates prostate growth through androgen receptor modulation

doi: 10.1038/s41598-017-15832-5

Figure Lengend Snippet: 5-HT, 5-Htr1a specific agonist and 5-Htr1b specific agonist down-regulates AR expression in rat ventral prostate. ( a ) Western blot analysis of AR expression in prostate explants treated with increasing doses of 5-HT, specific 5-Htr1a agonist, 8-OH-DPAT, and specific 5-Htr1b agonist, anpirtoline. ( b ) Quantification of AR protein in VPs treated with different concentrations of 5-HT in medium conditions without or with testosterone supplementation, n ≥ 3 (each sample contained a pool of 4 VPs). ( c ) Quantification of AR protein in VPs treated with different concentrations of 8-OH-DPAT in medium conditions without or with testosterone supplementation, n ≥ 3 (each sample contained a pool of 4 VPs). ( d ) Quantification of AR protein in VPs treated with different concentrations of Anpirtoline in medium conditions without or with testosterone supplementation, n ≥ 3 (each sample contained a pool of 4 VPs). Error bars indicate s.e.m. n.s . non-significant; * p

Article Snippet: The 5-Htr1a specific agonist, 8-OH-DPAT and the 5-Htr1b specific agonist, Anpirtoline, were purchased from Tocris-Bioscience (Bristol, UK).

Techniques: Expressing, Western Blot

Journal: Scientific Reports

Article Title: Serotonin regulates prostate growth through androgen receptor modulation

doi: 10.1038/s41598-017-15832-5

Figure Lengend Snippet: 5-HT, 5-Htr1a specific agonist and 5-Htr1b specific agonist inhibits cell viability in BPH-1 and WPMY-1 human prostatic cells without any effect in PNT1A cells. ( a , b , c ) Effect of 5-HT on cell viability analyzed by MTS assay in BPH-1, PNT1A and WPMY-1 cells. ( d ) Immunofluorescence analysis of 5-Htr1a and 5-Htr1b expression in BPH-1, PNT1A and WPMY-1 cells. ( e , f , g ) Effect of 5-Htr1a specific agonist, 8-OH-DPAT, and ( h , i , j ) 5-Htr1b specific agonist, Anpirtoline, on cell viability analyzed by MTS assay in BPH-1, PNT1A and WPMY-1 cells. The data are expressed relative to control condition (0 µM 5-HT without testosterone supplementation) and were reproduced in at least three independent experiments. Error bars indicate s.e.m. n.s . non-significant; 5-HT, serotonin; * p

Article Snippet: The 5-Htr1a specific agonist, 8-OH-DPAT and the 5-Htr1b specific agonist, Anpirtoline, were purchased from Tocris-Bioscience (Bristol, UK).

Techniques: MTS Assay, Immunofluorescence, Expressing

Journal: Scientific Reports

Article Title: Serotonin regulates prostate growth through androgen receptor modulation

doi: 10.1038/s41598-017-15832-5

Figure Lengend Snippet: 5-HT, 5-Htr1a specific agonist and 5-Htr1b specific agonist down-regulates AR expression in human prostatic cells. ( a ) Western blot analysis of AR expression in the three cell lines after 5-HT, 8-OH-DPAT and anpirtoline treatment. ( b ) Quantification of AR in BPH-1 and ( c ) WPMY-1 cells after 5-HT treatment in medium conditions without or with Testosterone supplementation. ( d ) Quantification of AR protein levels in BPH-1 and ( e ) WPMY-1 cells after 8-OH-DPAT treatment in medium conditions without or with Testosterone supplementation. ( f ) Quantification of AR protein levels in BPH-1 and ( g ) WPMY-1 cells after Anpirtoline treatment in medium conditions without or with Testosterone supplementation. The data are expressed relative to control condition (0 µM 5-HT without testosterone supplementation) and were reproduced in at least three independent experiments. Full, uncropped gel images are shown. Error bars indicate s.e.m. n.s . non-significant; * P

Article Snippet: The 5-Htr1a specific agonist, 8-OH-DPAT and the 5-Htr1b specific agonist, Anpirtoline, were purchased from Tocris-Bioscience (Bristol, UK).

Techniques: Expressing, Western Blot

Journal: Scientific Reports

Article Title: Serotonin regulates prostate growth through androgen receptor modulation

doi: 10.1038/s41598-017-15832-5

Figure Lengend Snippet: 5-HT, 5-Htr1a specific agonist and 5-Htr1b specific agonist inhibit prostate branching morphogenesis. ( a ) Photographs of representative VPs at D 0 and at D 4 of culture treated with different 5-HT concentrations. ( b ) Morphometric analysis of the effect of 5-HT on VPs area and ( c ) number of peripheral buds ( n ≥ 12 VPs per group). ( d ) Immunofluorescence analysis of 5-Htr1a and ( e ) 5-Htr1b expression in the rat prostate. ( f ) Photographs of representative VPs at D0 and at D4 of culture treated with different 8-OH-DPAT concentrations. ( g ) Morphometric analysis of the effect of 8-OH-DPAT on VPs area and ( h ) number of peripheral buds ( n ≥ 12 VPs per group). ( i ) Photographs of representative VPs at D0 and at D4 of culture treated with different anpirtoline concentrations. ( j ) Morphometric analysis of the effect of Anpirtoline on VPs area and ( k ) number of peripheral buds ( n ≥ 12 VPs per group). Error bars indicate s.e.m. *** p

Article Snippet: The 5-Htr1a specific agonist, 8-OH-DPAT and the 5-Htr1b specific agonist, Anpirtoline, were purchased from Tocris-Bioscience (Bristol, UK).

Techniques: Immunofluorescence, Expressing

Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

Article Title: Serum Total 25‐OH Vitamin D Adds Little Prognostic Value in Patients Undergoing Coronary Catheterization

doi: 10.1161/JAHA.116.004289

Figure Lengend Snippet: Associations between serum total 25‐ OH vitamin D quintile and mortality. Median 25‐ OH vitamin D is shown for each quintile. Demographic risk factors were age and sex. Clinical risk factors were body mass index, smoking status (current, prior vs never), renal disease, hypertension, hyperlipidemia, type 2 diabetes mellitus, family history of heart disease, prior myocardial infarction, congestive heart failure, whether ejection fraction was measured, ejection fraction (

Article Snippet: Because 25‐OH vitamin D was measured by liquid chromatography–tandem mass spectrometry in Edmonton and by immunoassay (Liaison; DiaSorin) in Calgary, 240 identical samples were run by each method (R 2 =0.8, slope=1.0, intercept=−8), and Edmonton results were corrected to match those from Calgary by subtracting 8 nmol/L.

Techniques: