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    Ensure flat even banding and crisp resolution with Thermo Scientific Owl P82 Dual Gel System Replacement Parts For use with the Owl P82 Dual Gel System parts include replacement plates
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    90
    Selleck Chemicals og l002
    JIB-04 demethylase inhibitor sensitizes B-ALL cells to dex-induced cell death. a Nalm6 cells were treated with JIB-04 at 0.5 μM, <t>OG-L002</t> at 10 μM or vehicle DMSO for 24 h. Lysates were analyzed by immunoblot with the indicated antibodies (left panel) or immunoprecipitated with pan methyllysine antibody and analyzed by immunoblot with G9a antibody (right panel). Results shown are representative of three independent experiments. b Nalm6 cells were treated with vehicle DMSO or with JIB-04 at 0.25 μM (left panel) or OG-L002 at 10 μM (right panel) for 72 h in addition to a serial dilution of dex, and cell survival was measured by fluorescence metabolic assay. The fluorescence intensity for dex-treated cells was normalized to that measured with ethanol-treated cells. Percentage of survival is shown as the mean ± SEM for 4 independent experiments (each performed with triplicate biological replicates) and p values for results at individual dex concentrations were calculated using a paired t -test; * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001. A F -test was also calculated to compare the two curves. Insets show the corresponding half-maximal effective concentration (EC 50 ) values. c Nalm6 cells were first treated with JIB-04 at 0.5 μM, OG-L002 at 10 μM or DMSO for 4 h, then 100 nM of dex or ethanol was added to the media for an additional 20 h. Lysates were analyzed by immunoblot with the indicated antibodies. Results shown are representative of three independent experiments
    Og L002, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 90/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Millipore octylglucoside og
    JIB-04 demethylase inhibitor sensitizes B-ALL cells to dex-induced cell death. a Nalm6 cells were treated with JIB-04 at 0.5 μM, <t>OG-L002</t> at 10 μM or vehicle DMSO for 24 h. Lysates were analyzed by immunoblot with the indicated antibodies (left panel) or immunoprecipitated with pan methyllysine antibody and analyzed by immunoblot with G9a antibody (right panel). Results shown are representative of three independent experiments. b Nalm6 cells were treated with vehicle DMSO or with JIB-04 at 0.25 μM (left panel) or OG-L002 at 10 μM (right panel) for 72 h in addition to a serial dilution of dex, and cell survival was measured by fluorescence metabolic assay. The fluorescence intensity for dex-treated cells was normalized to that measured with ethanol-treated cells. Percentage of survival is shown as the mean ± SEM for 4 independent experiments (each performed with triplicate biological replicates) and p values for results at individual dex concentrations were calculated using a paired t -test; * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001. A F -test was also calculated to compare the two curves. Insets show the corresponding half-maximal effective concentration (EC 50 ) values. c Nalm6 cells were first treated with JIB-04 at 0.5 μM, OG-L002 at 10 μM or DMSO for 4 h, then 100 nM of dex or ethanol was added to the media for an additional 20 h. Lysates were analyzed by immunoblot with the indicated antibodies. Results shown are representative of three independent experiments
    Octylglucoside Og, supplied by Millipore, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Glen Research og phosphoramidite
    JIB-04 demethylase inhibitor sensitizes B-ALL cells to dex-induced cell death. a Nalm6 cells were treated with JIB-04 at 0.5 μM, <t>OG-L002</t> at 10 μM or vehicle DMSO for 24 h. Lysates were analyzed by immunoblot with the indicated antibodies (left panel) or immunoprecipitated with pan methyllysine antibody and analyzed by immunoblot with G9a antibody (right panel). Results shown are representative of three independent experiments. b Nalm6 cells were treated with vehicle DMSO or with JIB-04 at 0.25 μM (left panel) or OG-L002 at 10 μM (right panel) for 72 h in addition to a serial dilution of dex, and cell survival was measured by fluorescence metabolic assay. The fluorescence intensity for dex-treated cells was normalized to that measured with ethanol-treated cells. Percentage of survival is shown as the mean ± SEM for 4 independent experiments (each performed with triplicate biological replicates) and p values for results at individual dex concentrations were calculated using a paired t -test; * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001. A F -test was also calculated to compare the two curves. Insets show the corresponding half-maximal effective concentration (EC 50 ) values. c Nalm6 cells were first treated with JIB-04 at 0.5 μM, OG-L002 at 10 μM or DMSO for 4 h, then 100 nM of dex or ethanol was added to the media for an additional 20 h. Lysates were analyzed by immunoblot with the indicated antibodies. Results shown are representative of three independent experiments
    Og Phosphoramidite, supplied by Glen Research, used in various techniques. Bioz Stars score: 80/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Thermo Fisher og wga
    JIB-04 demethylase inhibitor sensitizes B-ALL cells to dex-induced cell death. a Nalm6 cells were treated with JIB-04 at 0.5 μM, <t>OG-L002</t> at 10 μM or vehicle DMSO for 24 h. Lysates were analyzed by immunoblot with the indicated antibodies (left panel) or immunoprecipitated with pan methyllysine antibody and analyzed by immunoblot with G9a antibody (right panel). Results shown are representative of three independent experiments. b Nalm6 cells were treated with vehicle DMSO or with JIB-04 at 0.25 μM (left panel) or OG-L002 at 10 μM (right panel) for 72 h in addition to a serial dilution of dex, and cell survival was measured by fluorescence metabolic assay. The fluorescence intensity for dex-treated cells was normalized to that measured with ethanol-treated cells. Percentage of survival is shown as the mean ± SEM for 4 independent experiments (each performed with triplicate biological replicates) and p values for results at individual dex concentrations were calculated using a paired t -test; * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001. A F -test was also calculated to compare the two curves. Insets show the corresponding half-maximal effective concentration (EC 50 ) values. c Nalm6 cells were first treated with JIB-04 at 0.5 μM, OG-L002 at 10 μM or DMSO for 4 h, then 100 nM of dex or ethanol was added to the media for an additional 20 h. Lysates were analyzed by immunoblot with the indicated antibodies. Results shown are representative of three independent experiments
    Og Wga, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    82
    Brabender opitz og
    JIB-04 demethylase inhibitor sensitizes B-ALL cells to dex-induced cell death. a Nalm6 cells were treated with JIB-04 at 0.5 μM, <t>OG-L002</t> at 10 μM or vehicle DMSO for 24 h. Lysates were analyzed by immunoblot with the indicated antibodies (left panel) or immunoprecipitated with pan methyllysine antibody and analyzed by immunoblot with G9a antibody (right panel). Results shown are representative of three independent experiments. b Nalm6 cells were treated with vehicle DMSO or with JIB-04 at 0.25 μM (left panel) or OG-L002 at 10 μM (right panel) for 72 h in addition to a serial dilution of dex, and cell survival was measured by fluorescence metabolic assay. The fluorescence intensity for dex-treated cells was normalized to that measured with ethanol-treated cells. Percentage of survival is shown as the mean ± SEM for 4 independent experiments (each performed with triplicate biological replicates) and p values for results at individual dex concentrations were calculated using a paired t -test; * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001. A F -test was also calculated to compare the two curves. Insets show the corresponding half-maximal effective concentration (EC 50 ) values. c Nalm6 cells were first treated with JIB-04 at 0.5 μM, OG-L002 at 10 μM or DMSO for 4 h, then 100 nM of dex or ethanol was added to the media for an additional 20 h. Lysates were analyzed by immunoblot with the indicated antibodies. Results shown are representative of three independent experiments
    Opitz Og, supplied by Brabender, used in various techniques. Bioz Stars score: 82/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    75
    Anatrace og anagrade
    JIB-04 demethylase inhibitor sensitizes B-ALL cells to dex-induced cell death. a Nalm6 cells were treated with JIB-04 at 0.5 μM, <t>OG-L002</t> at 10 μM or vehicle DMSO for 24 h. Lysates were analyzed by immunoblot with the indicated antibodies (left panel) or immunoprecipitated with pan methyllysine antibody and analyzed by immunoblot with G9a antibody (right panel). Results shown are representative of three independent experiments. b Nalm6 cells were treated with vehicle DMSO or with JIB-04 at 0.25 μM (left panel) or OG-L002 at 10 μM (right panel) for 72 h in addition to a serial dilution of dex, and cell survival was measured by fluorescence metabolic assay. The fluorescence intensity for dex-treated cells was normalized to that measured with ethanol-treated cells. Percentage of survival is shown as the mean ± SEM for 4 independent experiments (each performed with triplicate biological replicates) and p values for results at individual dex concentrations were calculated using a paired t -test; * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001. A F -test was also calculated to compare the two curves. Insets show the corresponding half-maximal effective concentration (EC 50 ) values. c Nalm6 cells were first treated with JIB-04 at 0.5 μM, OG-L002 at 10 μM or DMSO for 4 h, then 100 nM of dex or ethanol was added to the media for an additional 20 h. Lysates were analyzed by immunoblot with the indicated antibodies. Results shown are representative of three independent experiments
    Og Anagrade, supplied by Anatrace, used in various techniques. Bioz Stars score: 75/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Millipore octylglucopyranoside og
    ( a ) ω-Thiolipid (1-oleoyl-2-(16-thiopalmitoyl)-sn-glycero-3-phosphocholine); ( b ) surface plasmon resonance data. Bare gold chips were mounted in a Biacore X-100 and thiolipid 1 mg ml −1 in 1% <t>octylglucopyranoside,</t> 50 mM Tris pH 8.0, 10 mM TCEP was injected at a flow rate of 5 μl min −1 for 10 min, after which the surface was washed with 1% v/v SDS for 100 s before repeating the cycle 12 times. The amount of thiolipid bound after each injection was determined by the increase in resonance units each wash; these successive values were plotted against injection number ( c ) and fitted to estimate the maximum saturation coverage.
    Octylglucopyranoside Og, supplied by Millipore, used in various techniques. Bioz Stars score: 77/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    DNA Genotek oragene og 500
    ( a ) ω-Thiolipid (1-oleoyl-2-(16-thiopalmitoyl)-sn-glycero-3-phosphocholine); ( b ) surface plasmon resonance data. Bare gold chips were mounted in a Biacore X-100 and thiolipid 1 mg ml −1 in 1% <t>octylglucopyranoside,</t> 50 mM Tris pH 8.0, 10 mM TCEP was injected at a flow rate of 5 μl min −1 for 10 min, after which the surface was washed with 1% v/v SDS for 100 s before repeating the cycle 12 times. The amount of thiolipid bound after each injection was determined by the increase in resonance units each wash; these successive values were plotted against injection number ( c ) and fitted to estimate the maximum saturation coverage.
    Oragene Og 500, supplied by DNA Genotek, used in various techniques. Bioz Stars score: 79/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    87
    Anatrace octyl glucopyranoside og
    ( a ) ω-Thiolipid (1-oleoyl-2-(16-thiopalmitoyl)-sn-glycero-3-phosphocholine); ( b ) surface plasmon resonance data. Bare gold chips were mounted in a Biacore X-100 and thiolipid 1 mg ml −1 in 1% <t>octylglucopyranoside,</t> 50 mM Tris pH 8.0, 10 mM TCEP was injected at a flow rate of 5 μl min −1 for 10 min, after which the surface was washed with 1% v/v SDS for 100 s before repeating the cycle 12 times. The amount of thiolipid bound after each injection was determined by the increase in resonance units each wash; these successive values were plotted against injection number ( c ) and fitted to estimate the maximum saturation coverage.
    Octyl Glucopyranoside Og, supplied by Anatrace, used in various techniques. Bioz Stars score: 87/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Frontier Scientific oxalylglycine og
    ( a ) ω-Thiolipid (1-oleoyl-2-(16-thiopalmitoyl)-sn-glycero-3-phosphocholine); ( b ) surface plasmon resonance data. Bare gold chips were mounted in a Biacore X-100 and thiolipid 1 mg ml −1 in 1% <t>octylglucopyranoside,</t> 50 mM Tris pH 8.0, 10 mM TCEP was injected at a flow rate of 5 μl min −1 for 10 min, after which the surface was washed with 1% v/v SDS for 100 s before repeating the cycle 12 times. The amount of thiolipid bound after each injection was determined by the increase in resonance units each wash; these successive values were plotted against injection number ( c ) and fitted to estimate the maximum saturation coverage.
    Oxalylglycine Og, supplied by Frontier Scientific, used in various techniques. Bioz Stars score: 77/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    DNA Genotek oragene og 300
    ( a ) ω-Thiolipid (1-oleoyl-2-(16-thiopalmitoyl)-sn-glycero-3-phosphocholine); ( b ) surface plasmon resonance data. Bare gold chips were mounted in a Biacore X-100 and thiolipid 1 mg ml −1 in 1% <t>octylglucopyranoside,</t> 50 mM Tris pH 8.0, 10 mM TCEP was injected at a flow rate of 5 μl min −1 for 10 min, after which the surface was washed with 1% v/v SDS for 100 s before repeating the cycle 12 times. The amount of thiolipid bound after each injection was determined by the increase in resonance units each wash; these successive values were plotted against injection number ( c ) and fitted to estimate the maximum saturation coverage.
    Oragene Og 300, supplied by DNA Genotek, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Anatrace n octylglucoside og
    ( a ) ω-Thiolipid (1-oleoyl-2-(16-thiopalmitoyl)-sn-glycero-3-phosphocholine); ( b ) surface plasmon resonance data. Bare gold chips were mounted in a Biacore X-100 and thiolipid 1 mg ml −1 in 1% <t>octylglucopyranoside,</t> 50 mM Tris pH 8.0, 10 mM TCEP was injected at a flow rate of 5 μl min −1 for 10 min, after which the surface was washed with 1% v/v SDS for 100 s before repeating the cycle 12 times. The amount of thiolipid bound after each injection was determined by the increase in resonance units each wash; these successive values were plotted against injection number ( c ) and fitted to estimate the maximum saturation coverage.
    N Octylglucoside Og, supplied by Anatrace, used in various techniques. Bioz Stars score: 79/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Carbosynth β og
    The electronic absorption (lower panel) and circular dichroism (upper panel) spectra of the B780 (blue), B820 (orange) and B870 (dark pink) oligomeric forms of LH1 in 5%, 1% and 0.4% aqueous <t>β-OG,</t> respectively, recorded at room temperature. The absorption spectra were normalized to the maximum of the Soret band whereas the circular dichroism spectra to the absorption intensity of the Q Y maximum. For comparison, also the absorption spectrum of monomeric bacteriochlorophyll a (BChl a ) in acetone is shown (black dotted line). The shaded areas denote the integrated intensities of the Q Y transitions.
    β Og, supplied by Carbosynth, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Thermo Fisher oregon green og
    The electronic absorption (lower panel) and circular dichroism (upper panel) spectra of the B780 (blue), B820 (orange) and B870 (dark pink) oligomeric forms of LH1 in 5%, 1% and 0.4% aqueous <t>β-OG,</t> respectively, recorded at room temperature. The absorption spectra were normalized to the maximum of the Soret band whereas the circular dichroism spectra to the absorption intensity of the Q Y maximum. For comparison, also the absorption spectrum of monomeric bacteriochlorophyll a (BChl a ) in acetone is shown (black dotted line). The shaded areas denote the integrated intensities of the Q Y transitions.
    Oregon Green Og, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    75
    Thermo Fisher og 488 x succinimidylester og
    The electronic absorption (lower panel) and circular dichroism (upper panel) spectra of the B780 (blue), B820 (orange) and B870 (dark pink) oligomeric forms of LH1 in 5%, 1% and 0.4% aqueous <t>β-OG,</t> respectively, recorded at room temperature. The absorption spectra were normalized to the maximum of the Soret band whereas the circular dichroism spectra to the absorption intensity of the Q Y maximum. For comparison, also the absorption spectrum of monomeric bacteriochlorophyll a (BChl a ) in acetone is shown (black dotted line). The shaded areas denote the integrated intensities of the Q Y transitions.
    Og 488 X Succinimidylester Og, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 75/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher og 488 iodoacetamide
    The electronic absorption (lower panel) and circular dichroism (upper panel) spectra of the B780 (blue), B820 (orange) and B870 (dark pink) oligomeric forms of LH1 in 5%, 1% and 0.4% aqueous <t>β-OG,</t> respectively, recorded at room temperature. The absorption spectra were normalized to the maximum of the Soret band whereas the circular dichroism spectra to the absorption intensity of the Q Y maximum. For comparison, also the absorption spectrum of monomeric bacteriochlorophyll a (BChl a ) in acetone is shown (black dotted line). The shaded areas denote the integrated intensities of the Q Y transitions.
    Og 488 Iodoacetamide, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    76
    Thermo Fisher og labelled gelatin
    The electronic absorption (lower panel) and circular dichroism (upper panel) spectra of the B780 (blue), B820 (orange) and B870 (dark pink) oligomeric forms of LH1 in 5%, 1% and 0.4% aqueous <t>β-OG,</t> respectively, recorded at room temperature. The absorption spectra were normalized to the maximum of the Soret band whereas the circular dichroism spectra to the absorption intensity of the Q Y maximum. For comparison, also the absorption spectrum of monomeric bacteriochlorophyll a (BChl a ) in acetone is shown (black dotted line). The shaded areas denote the integrated intensities of the Q Y transitions.
    Og Labelled Gelatin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 76/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    DNA Genotek oragene og 500 kit
    The electronic absorption (lower panel) and circular dichroism (upper panel) spectra of the B780 (blue), B820 (orange) and B870 (dark pink) oligomeric forms of LH1 in 5%, 1% and 0.4% aqueous <t>β-OG,</t> respectively, recorded at room temperature. The absorption spectra were normalized to the maximum of the Soret band whereas the circular dichroism spectra to the absorption intensity of the Q Y maximum. For comparison, also the absorption spectrum of monomeric bacteriochlorophyll a (BChl a ) in acetone is shown (black dotted line). The shaded areas denote the integrated intensities of the Q Y transitions.
    Oragene Og 500 Kit, supplied by DNA Genotek, used in various techniques. Bioz Stars score: 85/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    75
    Thermo Fisher oregon green carboxylate og
    The electronic absorption (lower panel) and circular dichroism (upper panel) spectra of the B780 (blue), B820 (orange) and B870 (dark pink) oligomeric forms of LH1 in 5%, 1% and 0.4% aqueous <t>β-OG,</t> respectively, recorded at room temperature. The absorption spectra were normalized to the maximum of the Soret band whereas the circular dichroism spectra to the absorption intensity of the Q Y maximum. For comparison, also the absorption spectrum of monomeric bacteriochlorophyll a (BChl a ) in acetone is shown (black dotted line). The shaded areas denote the integrated intensities of the Q Y transitions.
    Oregon Green Carboxylate Og, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 75/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    DNA Genotek og 250 commercial kits
    The electronic absorption (lower panel) and circular dichroism (upper panel) spectra of the B780 (blue), B820 (orange) and B870 (dark pink) oligomeric forms of LH1 in 5%, 1% and 0.4% aqueous <t>β-OG,</t> respectively, recorded at room temperature. The absorption spectra were normalized to the maximum of the Soret band whereas the circular dichroism spectra to the absorption intensity of the Q Y maximum. For comparison, also the absorption spectrum of monomeric bacteriochlorophyll a (BChl a ) in acetone is shown (black dotted line). The shaded areas denote the integrated intensities of the Q Y transitions.
    Og 250 Commercial Kits, supplied by DNA Genotek, used in various techniques. Bioz Stars score: 79/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    DNA Genotek og 250 oragene kit
    The electronic absorption (lower panel) and circular dichroism (upper panel) spectra of the B780 (blue), B820 (orange) and B870 (dark pink) oligomeric forms of LH1 in 5%, 1% and 0.4% aqueous <t>β-OG,</t> respectively, recorded at room temperature. The absorption spectra were normalized to the maximum of the Soret band whereas the circular dichroism spectra to the absorption intensity of the Q Y maximum. For comparison, also the absorption spectrum of monomeric bacteriochlorophyll a (BChl a ) in acetone is shown (black dotted line). The shaded areas denote the integrated intensities of the Q Y transitions.
    Og 250 Oragene Kit, supplied by DNA Genotek, used in various techniques. Bioz Stars score: 78/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    82
    Addgene inc paav ef1a dio og
    Monosynaptic tracing initiated from spinal Grp::Cre neurons. A , A' , Grp::Cre neurons are located in lamina II of the spinal cord. The neuropil and cell bodies of GRP::Cre neurons overlap with central terminals of IB4+ and TH+ primary afferent fibers. B , High-resolution imaging of a tdTomato+ Grp neuron (red) indicates the presence of Homer1+ (green) postsynapses on Grp neurons, which are near IB4+ (blue) terminals. C , High-resolution imaging of tdTomato+ Grp neuropil (red) indicates the presence of Homer1+ (blue) postsynapses on Grp neurons near vGlut3+ (green) primary afferent terminals. D , Rabies-mediated monosynaptic retrograde tracing reveals many primary (mCherry+ and eGFP+) and secondary (only eGFP+) infected spinal neurons. E , Secondary infected DRG neurons are mostly NF200+ (arrows). F , Quantification of eGFP-labeled DRG neuron subtypes after retrograde transduction mediated by either SAD-G or oG rabies glycoprotein. SAD.RabiesΔG-eGFP (Enva) was always injected at 3.5 × 10 8 focus forming units per milliliter. <t>AAV1.EF1a.flex.mCherry-2A-SADG</t> and <t>pAAV1.Ef1a.DIO.oG.WPRE.hGH</t> were injected at 9.5 × 10 12 GC/ml. Scale bars: A , D , E , 100 μm; B , C , 5 μm. Error bars represent the SD.
    Paav Ef1a Dio Og, supplied by Addgene inc, used in various techniques. Bioz Stars score: 82/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Monosynaptic tracing initiated from spinal Grp::Cre neurons. A , A' , Grp::Cre neurons are located in lamina II of the spinal cord. The neuropil and cell bodies of GRP::Cre neurons overlap with central terminals of IB4+ and TH+ primary afferent fibers. B , High-resolution imaging of a tdTomato+ Grp neuron (red) indicates the presence of Homer1+ (green) postsynapses on Grp neurons, which are near IB4+ (blue) terminals. C , High-resolution imaging of tdTomato+ Grp neuropil (red) indicates the presence of Homer1+ (blue) postsynapses on Grp neurons near vGlut3+ (green) primary afferent terminals. D , Rabies-mediated monosynaptic retrograde tracing reveals many primary (mCherry+ and eGFP+) and secondary (only eGFP+) infected spinal neurons. E , Secondary infected DRG neurons are mostly NF200+ (arrows). F , Quantification of eGFP-labeled DRG neuron subtypes after retrograde transduction mediated by either SAD-G or oG rabies glycoprotein. SAD.RabiesΔG-eGFP (Enva) was always injected at 3.5 × 10 8 focus forming units per milliliter. <t>AAV1.EF1a.flex.mCherry-2A-SADG</t> and <t>pAAV1.Ef1a.DIO.oG.WPRE.hGH</t> were injected at 9.5 × 10 12 GC/ml. Scale bars: A , D , E , 100 μm; B , C , 5 μm. Error bars represent the SD.
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    Monosynaptic tracing initiated from spinal Grp::Cre neurons. A , A' , Grp::Cre neurons are located in lamina II of the spinal cord. The neuropil and cell bodies of GRP::Cre neurons overlap with central terminals of IB4+ and TH+ primary afferent fibers. B , High-resolution imaging of a tdTomato+ Grp neuron (red) indicates the presence of Homer1+ (green) postsynapses on Grp neurons, which are near IB4+ (blue) terminals. C , High-resolution imaging of tdTomato+ Grp neuropil (red) indicates the presence of Homer1+ (blue) postsynapses on Grp neurons near vGlut3+ (green) primary afferent terminals. D , Rabies-mediated monosynaptic retrograde tracing reveals many primary (mCherry+ and eGFP+) and secondary (only eGFP+) infected spinal neurons. E , Secondary infected DRG neurons are mostly NF200+ (arrows). F , Quantification of eGFP-labeled DRG neuron subtypes after retrograde transduction mediated by either SAD-G or oG rabies glycoprotein. SAD.RabiesΔG-eGFP (Enva) was always injected at 3.5 × 10 8 focus forming units per milliliter. <t>AAV1.EF1a.flex.mCherry-2A-SADG</t> and <t>pAAV1.Ef1a.DIO.oG.WPRE.hGH</t> were injected at 9.5 × 10 12 GC/ml. Scale bars: A , D , E , 100 μm; B , C , 5 μm. Error bars represent the SD.
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    Monosynaptic tracing initiated from spinal Grp::Cre neurons. A , A' , Grp::Cre neurons are located in lamina II of the spinal cord. The neuropil and cell bodies of GRP::Cre neurons overlap with central terminals of IB4+ and TH+ primary afferent fibers. B , High-resolution imaging of a tdTomato+ Grp neuron (red) indicates the presence of Homer1+ (green) postsynapses on Grp neurons, which are near IB4+ (blue) terminals. C , High-resolution imaging of tdTomato+ Grp neuropil (red) indicates the presence of Homer1+ (blue) postsynapses on Grp neurons near vGlut3+ (green) primary afferent terminals. D , Rabies-mediated monosynaptic retrograde tracing reveals many primary (mCherry+ and eGFP+) and secondary (only eGFP+) infected spinal neurons. E , Secondary infected DRG neurons are mostly NF200+ (arrows). F , Quantification of eGFP-labeled DRG neuron subtypes after retrograde transduction mediated by either SAD-G or oG rabies glycoprotein. SAD.RabiesΔG-eGFP (Enva) was always injected at 3.5 × 10 8 focus forming units per milliliter. <t>AAV1.EF1a.flex.mCherry-2A-SADG</t> and <t>pAAV1.Ef1a.DIO.oG.WPRE.hGH</t> were injected at 9.5 × 10 12 GC/ml. Scale bars: A , D , E , 100 μm; B , C , 5 μm. Error bars represent the SD.
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    Monosynaptic tracing initiated from spinal Grp::Cre neurons. A , A' , Grp::Cre neurons are located in lamina II of the spinal cord. The neuropil and cell bodies of GRP::Cre neurons overlap with central terminals of IB4+ and TH+ primary afferent fibers. B , High-resolution imaging of a tdTomato+ Grp neuron (red) indicates the presence of Homer1+ (green) postsynapses on Grp neurons, which are near IB4+ (blue) terminals. C , High-resolution imaging of tdTomato+ Grp neuropil (red) indicates the presence of Homer1+ (blue) postsynapses on Grp neurons near vGlut3+ (green) primary afferent terminals. D , Rabies-mediated monosynaptic retrograde tracing reveals many primary (mCherry+ and eGFP+) and secondary (only eGFP+) infected spinal neurons. E , Secondary infected DRG neurons are mostly NF200+ (arrows). F , Quantification of eGFP-labeled DRG neuron subtypes after retrograde transduction mediated by either SAD-G or oG rabies glycoprotein. SAD.RabiesΔG-eGFP (Enva) was always injected at 3.5 × 10 8 focus forming units per milliliter. <t>AAV1.EF1a.flex.mCherry-2A-SADG</t> and <t>pAAV1.Ef1a.DIO.oG.WPRE.hGH</t> were injected at 9.5 × 10 12 GC/ml. Scale bars: A , D , E , 100 μm; B , C , 5 μm. Error bars represent the SD.
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    Monosynaptic tracing initiated from spinal Grp::Cre neurons. A , A' , Grp::Cre neurons are located in lamina II of the spinal cord. The neuropil and cell bodies of GRP::Cre neurons overlap with central terminals of IB4+ and TH+ primary afferent fibers. B , High-resolution imaging of a tdTomato+ Grp neuron (red) indicates the presence of Homer1+ (green) postsynapses on Grp neurons, which are near IB4+ (blue) terminals. C , High-resolution imaging of tdTomato+ Grp neuropil (red) indicates the presence of Homer1+ (blue) postsynapses on Grp neurons near vGlut3+ (green) primary afferent terminals. D , Rabies-mediated monosynaptic retrograde tracing reveals many primary (mCherry+ and eGFP+) and secondary (only eGFP+) infected spinal neurons. E , Secondary infected DRG neurons are mostly NF200+ (arrows). F , Quantification of eGFP-labeled DRG neuron subtypes after retrograde transduction mediated by either SAD-G or oG rabies glycoprotein. SAD.RabiesΔG-eGFP (Enva) was always injected at 3.5 × 10 8 focus forming units per milliliter. <t>AAV1.EF1a.flex.mCherry-2A-SADG</t> and <t>pAAV1.Ef1a.DIO.oG.WPRE.hGH</t> were injected at 9.5 × 10 12 GC/ml. Scale bars: A , D , E , 100 μm; B , C , 5 μm. Error bars represent the SD.
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    Monosynaptic tracing initiated from spinal Grp::Cre neurons. A , A' , Grp::Cre neurons are located in lamina II of the spinal cord. The neuropil and cell bodies of GRP::Cre neurons overlap with central terminals of IB4+ and TH+ primary afferent fibers. B , High-resolution imaging of a tdTomato+ Grp neuron (red) indicates the presence of Homer1+ (green) postsynapses on Grp neurons, which are near IB4+ (blue) terminals. C , High-resolution imaging of tdTomato+ Grp neuropil (red) indicates the presence of Homer1+ (blue) postsynapses on Grp neurons near vGlut3+ (green) primary afferent terminals. D , Rabies-mediated monosynaptic retrograde tracing reveals many primary (mCherry+ and eGFP+) and secondary (only eGFP+) infected spinal neurons. E , Secondary infected DRG neurons are mostly NF200+ (arrows). F , Quantification of eGFP-labeled DRG neuron subtypes after retrograde transduction mediated by either SAD-G or oG rabies glycoprotein. SAD.RabiesΔG-eGFP (Enva) was always injected at 3.5 × 10 8 focus forming units per milliliter. <t>AAV1.EF1a.flex.mCherry-2A-SADG</t> and <t>pAAV1.Ef1a.DIO.oG.WPRE.hGH</t> were injected at 9.5 × 10 12 GC/ml. Scale bars: A , D , E , 100 μm; B , C , 5 μm. Error bars represent the SD.
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    Monosynaptic tracing initiated from spinal Grp::Cre neurons. A , A' , Grp::Cre neurons are located in lamina II of the spinal cord. The neuropil and cell bodies of GRP::Cre neurons overlap with central terminals of IB4+ and TH+ primary afferent fibers. B , High-resolution imaging of a tdTomato+ Grp neuron (red) indicates the presence of Homer1+ (green) postsynapses on Grp neurons, which are near IB4+ (blue) terminals. C , High-resolution imaging of tdTomato+ Grp neuropil (red) indicates the presence of Homer1+ (blue) postsynapses on Grp neurons near vGlut3+ (green) primary afferent terminals. D , Rabies-mediated monosynaptic retrograde tracing reveals many primary (mCherry+ and eGFP+) and secondary (only eGFP+) infected spinal neurons. E , Secondary infected DRG neurons are mostly NF200+ (arrows). F , Quantification of eGFP-labeled DRG neuron subtypes after retrograde transduction mediated by either SAD-G or oG rabies glycoprotein. SAD.RabiesΔG-eGFP (Enva) was always injected at 3.5 × 10 8 focus forming units per milliliter. <t>AAV1.EF1a.flex.mCherry-2A-SADG</t> and <t>pAAV1.Ef1a.DIO.oG.WPRE.hGH</t> were injected at 9.5 × 10 12 GC/ml. Scale bars: A , D , E , 100 μm; B , C , 5 μm. Error bars represent the SD.
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    Monosynaptic tracing initiated from spinal Grp::Cre neurons. A , A' , Grp::Cre neurons are located in lamina II of the spinal cord. The neuropil and cell bodies of GRP::Cre neurons overlap with central terminals of IB4+ and TH+ primary afferent fibers. B , High-resolution imaging of a tdTomato+ Grp neuron (red) indicates the presence of Homer1+ (green) postsynapses on Grp neurons, which are near IB4+ (blue) terminals. C , High-resolution imaging of tdTomato+ Grp neuropil (red) indicates the presence of Homer1+ (blue) postsynapses on Grp neurons near vGlut3+ (green) primary afferent terminals. D , Rabies-mediated monosynaptic retrograde tracing reveals many primary (mCherry+ and eGFP+) and secondary (only eGFP+) infected spinal neurons. E , Secondary infected DRG neurons are mostly NF200+ (arrows). F , Quantification of eGFP-labeled DRG neuron subtypes after retrograde transduction mediated by either SAD-G or oG rabies glycoprotein. SAD.RabiesΔG-eGFP (Enva) was always injected at 3.5 × 10 8 focus forming units per milliliter. <t>AAV1.EF1a.flex.mCherry-2A-SADG</t> and <t>pAAV1.Ef1a.DIO.oG.WPRE.hGH</t> were injected at 9.5 × 10 12 GC/ml. Scale bars: A , D , E , 100 μm; B , C , 5 μm. Error bars represent the SD.
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    Image Search Results


    JIB-04 demethylase inhibitor sensitizes B-ALL cells to dex-induced cell death. a Nalm6 cells were treated with JIB-04 at 0.5 μM, OG-L002 at 10 μM or vehicle DMSO for 24 h. Lysates were analyzed by immunoblot with the indicated antibodies (left panel) or immunoprecipitated with pan methyllysine antibody and analyzed by immunoblot with G9a antibody (right panel). Results shown are representative of three independent experiments. b Nalm6 cells were treated with vehicle DMSO or with JIB-04 at 0.25 μM (left panel) or OG-L002 at 10 μM (right panel) for 72 h in addition to a serial dilution of dex, and cell survival was measured by fluorescence metabolic assay. The fluorescence intensity for dex-treated cells was normalized to that measured with ethanol-treated cells. Percentage of survival is shown as the mean ± SEM for 4 independent experiments (each performed with triplicate biological replicates) and p values for results at individual dex concentrations were calculated using a paired t -test; * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001. A F -test was also calculated to compare the two curves. Insets show the corresponding half-maximal effective concentration (EC 50 ) values. c Nalm6 cells were first treated with JIB-04 at 0.5 μM, OG-L002 at 10 μM or DMSO for 4 h, then 100 nM of dex or ethanol was added to the media for an additional 20 h. Lysates were analyzed by immunoblot with the indicated antibodies. Results shown are representative of three independent experiments

    Journal: Cell Death & Disease

    Article Title: Increasing G9a automethylation sensitizes B acute lymphoblastic leukemia cells to glucocorticoid-induced death

    doi: 10.1038/s41419-018-1110-z

    Figure Lengend Snippet: JIB-04 demethylase inhibitor sensitizes B-ALL cells to dex-induced cell death. a Nalm6 cells were treated with JIB-04 at 0.5 μM, OG-L002 at 10 μM or vehicle DMSO for 24 h. Lysates were analyzed by immunoblot with the indicated antibodies (left panel) or immunoprecipitated with pan methyllysine antibody and analyzed by immunoblot with G9a antibody (right panel). Results shown are representative of three independent experiments. b Nalm6 cells were treated with vehicle DMSO or with JIB-04 at 0.25 μM (left panel) or OG-L002 at 10 μM (right panel) for 72 h in addition to a serial dilution of dex, and cell survival was measured by fluorescence metabolic assay. The fluorescence intensity for dex-treated cells was normalized to that measured with ethanol-treated cells. Percentage of survival is shown as the mean ± SEM for 4 independent experiments (each performed with triplicate biological replicates) and p values for results at individual dex concentrations were calculated using a paired t -test; * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001. A F -test was also calculated to compare the two curves. Insets show the corresponding half-maximal effective concentration (EC 50 ) values. c Nalm6 cells were first treated with JIB-04 at 0.5 μM, OG-L002 at 10 μM or DMSO for 4 h, then 100 nM of dex or ethanol was added to the media for an additional 20 h. Lysates were analyzed by immunoblot with the indicated antibodies. Results shown are representative of three independent experiments

    Article Snippet: JIB-04 alone and OG-L002 alone caused slight increases in cleavage, presumably due to some cell toxicity.

    Techniques: Immunoprecipitation, Serial Dilution, Fluorescence, Metabolic Assay, Concentration Assay

    ( a ) ω-Thiolipid (1-oleoyl-2-(16-thiopalmitoyl)-sn-glycero-3-phosphocholine); ( b ) surface plasmon resonance data. Bare gold chips were mounted in a Biacore X-100 and thiolipid 1 mg ml −1 in 1% octylglucopyranoside, 50 mM Tris pH 8.0, 10 mM TCEP was injected at a flow rate of 5 μl min −1 for 10 min, after which the surface was washed with 1% v/v SDS for 100 s before repeating the cycle 12 times. The amount of thiolipid bound after each injection was determined by the increase in resonance units each wash; these successive values were plotted against injection number ( c ) and fitted to estimate the maximum saturation coverage.

    Journal: Journal of the Royal Society Interface

    Article Title: High coverage fluid-phase floating lipid bilayers supported by ω-thiolipid self-assembled monolayers

    doi: 10.1098/rsif.2014.0447

    Figure Lengend Snippet: ( a ) ω-Thiolipid (1-oleoyl-2-(16-thiopalmitoyl)-sn-glycero-3-phosphocholine); ( b ) surface plasmon resonance data. Bare gold chips were mounted in a Biacore X-100 and thiolipid 1 mg ml −1 in 1% octylglucopyranoside, 50 mM Tris pH 8.0, 10 mM TCEP was injected at a flow rate of 5 μl min −1 for 10 min, after which the surface was washed with 1% v/v SDS for 100 s before repeating the cycle 12 times. The amount of thiolipid bound after each injection was determined by the increase in resonance units each wash; these successive values were plotted against injection number ( c ) and fitted to estimate the maximum saturation coverage.

    Article Snippet: Fabrication of the ω-thiolipid self-assembled monolayer ω-Thiolipid (1-oleoyl-2-(16-thiopalmitoyl)-sn-glycero-3-phosphocholine; Avanti Polar Lipids; a ) was dissolved at 1 mg ml−1 in chloroform/methanol (4 : 1), dried as a film in a glass tube and resuspended in 1% octylglucopyranoside (OG) (Sigma), 50 mM Tris pH = 8.0 (OG buffer).

    Techniques: SPR Assay, Injection, Flow Cytometry

    The electronic absorption (lower panel) and circular dichroism (upper panel) spectra of the B780 (blue), B820 (orange) and B870 (dark pink) oligomeric forms of LH1 in 5%, 1% and 0.4% aqueous β-OG, respectively, recorded at room temperature. The absorption spectra were normalized to the maximum of the Soret band whereas the circular dichroism spectra to the absorption intensity of the Q Y maximum. For comparison, also the absorption spectrum of monomeric bacteriochlorophyll a (BChl a ) in acetone is shown (black dotted line). The shaded areas denote the integrated intensities of the Q Y transitions.

    Journal: Materials

    Article Title: Tuning the Photophysical Features of Self-Assembling Photoactive Polypeptides for Light-Harvesting

    doi: 10.3390/ma12213554

    Figure Lengend Snippet: The electronic absorption (lower panel) and circular dichroism (upper panel) spectra of the B780 (blue), B820 (orange) and B870 (dark pink) oligomeric forms of LH1 in 5%, 1% and 0.4% aqueous β-OG, respectively, recorded at room temperature. The absorption spectra were normalized to the maximum of the Soret band whereas the circular dichroism spectra to the absorption intensity of the Q Y maximum. For comparison, also the absorption spectrum of monomeric bacteriochlorophyll a (BChl a ) in acetone is shown (black dotted line). The shaded areas denote the integrated intensities of the Q Y transitions.

    Article Snippet: The pellet of the RC-depleted chromatophores was solubilized in 2.35% (w /v ) β-OG (Carbosynth, Compton, UK) in 20 mM Tris-HCl, pH 7.8, and loaded on a DEAE-cellulose (DE52, Whatman, Maidstone, UK) column pre-equilibrated in 0.8% β-OG (w /v ).

    Techniques:

    Monosynaptic tracing initiated from spinal Grp::Cre neurons. A , A' , Grp::Cre neurons are located in lamina II of the spinal cord. The neuropil and cell bodies of GRP::Cre neurons overlap with central terminals of IB4+ and TH+ primary afferent fibers. B , High-resolution imaging of a tdTomato+ Grp neuron (red) indicates the presence of Homer1+ (green) postsynapses on Grp neurons, which are near IB4+ (blue) terminals. C , High-resolution imaging of tdTomato+ Grp neuropil (red) indicates the presence of Homer1+ (blue) postsynapses on Grp neurons near vGlut3+ (green) primary afferent terminals. D , Rabies-mediated monosynaptic retrograde tracing reveals many primary (mCherry+ and eGFP+) and secondary (only eGFP+) infected spinal neurons. E , Secondary infected DRG neurons are mostly NF200+ (arrows). F , Quantification of eGFP-labeled DRG neuron subtypes after retrograde transduction mediated by either SAD-G or oG rabies glycoprotein. SAD.RabiesΔG-eGFP (Enva) was always injected at 3.5 × 10 8 focus forming units per milliliter. AAV1.EF1a.flex.mCherry-2A-SADG and pAAV1.Ef1a.DIO.oG.WPRE.hGH were injected at 9.5 × 10 12 GC/ml. Scale bars: A , D , E , 100 μm; B , C , 5 μm. Error bars represent the SD.

    Journal: The Journal of Neuroscience

    Article Title: Identification of Two Classes of Somatosensory Neurons That Display Resistance to Retrograde Infection by Rabies Virus

    doi: 10.1523/JNEUROSCI.1277-17.2017

    Figure Lengend Snippet: Monosynaptic tracing initiated from spinal Grp::Cre neurons. A , A' , Grp::Cre neurons are located in lamina II of the spinal cord. The neuropil and cell bodies of GRP::Cre neurons overlap with central terminals of IB4+ and TH+ primary afferent fibers. B , High-resolution imaging of a tdTomato+ Grp neuron (red) indicates the presence of Homer1+ (green) postsynapses on Grp neurons, which are near IB4+ (blue) terminals. C , High-resolution imaging of tdTomato+ Grp neuropil (red) indicates the presence of Homer1+ (blue) postsynapses on Grp neurons near vGlut3+ (green) primary afferent terminals. D , Rabies-mediated monosynaptic retrograde tracing reveals many primary (mCherry+ and eGFP+) and secondary (only eGFP+) infected spinal neurons. E , Secondary infected DRG neurons are mostly NF200+ (arrows). F , Quantification of eGFP-labeled DRG neuron subtypes after retrograde transduction mediated by either SAD-G or oG rabies glycoprotein. SAD.RabiesΔG-eGFP (Enva) was always injected at 3.5 × 10 8 focus forming units per milliliter. AAV1.EF1a.flex.mCherry-2A-SADG and pAAV1.Ef1a.DIO.oG.WPRE.hGH were injected at 9.5 × 10 12 GC/ml. Scale bars: A , D , E , 100 μm; B , C , 5 μm. Error bars represent the SD.

    Article Snippet: AAV.flex.mCherry-2A-RabG vector was cloned by excising the ChR2-mCherry fusion protein from pAAV-Ef1a-DIO-hChR2(H134R)-mCherryWPRE-pA (kindly provided by Dr. Karl Deisseroth, Stanford University) with AscI and NheI and replacing it with PCR amplified mCherry-2A-RabG cDNA. pAAV-Ef1a-DIO-oG-WPRE-hGH was obtained from Addgene (74290; ) and packaged by the Viral Vector Facility (Zurich).

    Techniques: Imaging, Retrograde Tracing, Infection, Labeling, Transduction, Injection