Journal: BMC Microbiology
Article Title: Role of the SaeRS two-component regulatory system in Staphylococcus epidermidis autolysis and biofilm formation
Figure Lengend Snippet: Quantification of eDNA in SE1457 ΔsaeRS , SE1457, and SE1457 saec biofilms . eDNA was extracted from the unwashed 24 h biofilms of SE1457 ΔsaeRS (white bars), SE1457 (black bars), and SE1457 saec (gray bars). The eDNA in each biofilm was quantified by qPCR using primers specific for gyrA, serp0306, lysA , and leuA [ 19 , 28 ]. The quantity of eDNA was calculated as follows: total eDNA (ng)/relative OD600. Results represent the mean ± SD of three independent experiments. WT, SE1457; SAE, SE1457 ΔsaeRS ; SAEC, SE1457 saec.
Article Snippet: The OD600 of the cultures was measured at 60 min intervals for 12 h. For anaerobic growth conditions, bacteria were cultured in the Eppendorf tubes that were filled up with the TSB medium and sealed with wax.
Techniques: Real-time Polymerase Chain Reaction