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    BioTek Instruments od600
    Od600, supplied by BioTek Instruments, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/od600/product/BioTek Instruments
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    od600 - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    86
    Tecan Systems od600
    <t>OD600</t> time course of TOP10-rfp-lys grown in 15 ml tubes upon induction with HSL 100 nM (A) and RFP fluorescence time course in the supernatant (B) . Culture absorbance (A) and supernatant fluorescence (B) of TOP10-rfp-lys induced with HSL 100 nM (blue line). Uninduced TOP10-rfp-lys (red line), TOP10 bearing pHC-RFP induced with HSL 100 nM (green line) or uninduced (black line) are the negative controls. Induction was carried out in the exponential phase at OD600~0.55. Error bars represent the 95% confidence interval of the estimated mean.
    Od600, supplied by Tecan Systems, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/od600/product/Tecan Systems
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    od600 - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    86
    Eppendorf AG od600
    S. epidermidis attachment to polystyrene surfaces in the presence or absence of DNase I . (A) Attached SE1457 ΔatlE , SE1457 ΔsaeRS , SE1457 and SE1457 saec cells were observed by microscopy. Briefly, cell suspensions from the mid-exponential phase were diluted to <t>OD600</t> = 0.1 in PBS and then incubated in wells (1 mL per well) of cell-culture polystyrene chambers (Nunc, Roskilde, Denmark) with DNase I (140 U/mL) for 2 h at 37°C. S. epidermidis cells attached to the polystyrene surface were counted under microscope (400× magnification). (B) The number of attached bacteria per field was then counted. Results represent the mean ± SD of three independent experiments. *, P
    Od600, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/od600/product/Eppendorf AG
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    od600 - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    86
    GE Healthcare od600
    Growth curves of P. fluorescens strains cLP6a and cLP6a-1 . Growth of P. fluorescens strains cLP6a and cLP6a-1 at (a) 10°C, (b) 28°C or (c) 35°C determined as <t>OD600</t> Each data point is the mean of three independent cultures, and error bars, where visible, indicate the standard deviation.
    Od600, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/od600/product/GE Healthcare
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    od600 - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    Image Search Results


    OD600 time course of TOP10-rfp-lys grown in 15 ml tubes upon induction with HSL 100 nM (A) and RFP fluorescence time course in the supernatant (B) . Culture absorbance (A) and supernatant fluorescence (B) of TOP10-rfp-lys induced with HSL 100 nM (blue line). Uninduced TOP10-rfp-lys (red line), TOP10 bearing pHC-RFP induced with HSL 100 nM (green line) or uninduced (black line) are the negative controls. Induction was carried out in the exponential phase at OD600~0.55. Error bars represent the 95% confidence interval of the estimated mean.

    Journal: Journal of Biological Engineering

    Article Title: Characterization of a synthetic bacterial self-destruction device for programmed cell death and for recombinant proteins release

    doi: 10.1186/1754-1611-5-8

    Figure Lengend Snippet: OD600 time course of TOP10-rfp-lys grown in 15 ml tubes upon induction with HSL 100 nM (A) and RFP fluorescence time course in the supernatant (B) . Culture absorbance (A) and supernatant fluorescence (B) of TOP10-rfp-lys induced with HSL 100 nM (blue line). Uninduced TOP10-rfp-lys (red line), TOP10 bearing pHC-RFP induced with HSL 100 nM (green line) or uninduced (black line) are the negative controls. Induction was carried out in the exponential phase at OD600~0.55. Error bars represent the 95% confidence interval of the estimated mean.

    Article Snippet: After that time, a 200 μl aliquot of each culture was transferred in a flat-bottomed 96-well microplate (Greiner) and the OD600 was measured with an Infinite F200 microplate reader (Tecan).

    Techniques: Fluorescence

    Lysis profile of TOP10 bearing the lysis device in low copy number when induced in different growth phases in microplate reader . OD600 of TOP10 with pLC-T4LysHSL induced with HSL 100 nM (blue line) and uninduced (red line), pLC-T4Lys - induced with HSL 100 nM (green line) and uninduced (black line). Induction was performed in exponential phase (OD600 = 0.2) at t = 0 (A), early stationary phase (OD600~1.3) at t = 4 h (B) and late stationary phase (OD600~2) at t = 20 h (C). Error bars represent the 95% confidence interval of the estimated mean. For clarity of presentation, data points shown here are resampled with a 1-hour sampling time.

    Journal: Journal of Biological Engineering

    Article Title: Characterization of a synthetic bacterial self-destruction device for programmed cell death and for recombinant proteins release

    doi: 10.1186/1754-1611-5-8

    Figure Lengend Snippet: Lysis profile of TOP10 bearing the lysis device in low copy number when induced in different growth phases in microplate reader . OD600 of TOP10 with pLC-T4LysHSL induced with HSL 100 nM (blue line) and uninduced (red line), pLC-T4Lys - induced with HSL 100 nM (green line) and uninduced (black line). Induction was performed in exponential phase (OD600 = 0.2) at t = 0 (A), early stationary phase (OD600~1.3) at t = 4 h (B) and late stationary phase (OD600~2) at t = 20 h (C). Error bars represent the 95% confidence interval of the estimated mean. For clarity of presentation, data points shown here are resampled with a 1-hour sampling time.

    Article Snippet: After that time, a 200 μl aliquot of each culture was transferred in a flat-bottomed 96-well microplate (Greiner) and the OD600 was measured with an Infinite F200 microplate reader (Tecan).

    Techniques: Lysis, Low Copy Number, Planar Chromatography, Sampling

    Transfer function, rise time and delay time of the HSL-inducible lysis device in low copy plasmid in early stationary phase in microplate reader . Lysis entity of TOP10 cells with pLC-T4LysHSL induced with different concentrations of HSL in early stationary phase at OD600 = 0.9 (A). The experimental data (circles) were fitted with a Hill function (line, Vmax = 76, K 50 = 0.37, n = 1.3). For each concentration, the rise time, i.e. the time to rise from the 10% to 90% of the lysis entity (B) and the delay time before the OD600 drop after induction (C) are also shown. Error bars represent the 95% confidence interval of the estimated mean.

    Journal: Journal of Biological Engineering

    Article Title: Characterization of a synthetic bacterial self-destruction device for programmed cell death and for recombinant proteins release

    doi: 10.1186/1754-1611-5-8

    Figure Lengend Snippet: Transfer function, rise time and delay time of the HSL-inducible lysis device in low copy plasmid in early stationary phase in microplate reader . Lysis entity of TOP10 cells with pLC-T4LysHSL induced with different concentrations of HSL in early stationary phase at OD600 = 0.9 (A). The experimental data (circles) were fitted with a Hill function (line, Vmax = 76, K 50 = 0.37, n = 1.3). For each concentration, the rise time, i.e. the time to rise from the 10% to 90% of the lysis entity (B) and the delay time before the OD600 drop after induction (C) are also shown. Error bars represent the 95% confidence interval of the estimated mean.

    Article Snippet: After that time, a 200 μl aliquot of each culture was transferred in a flat-bottomed 96-well microplate (Greiner) and the OD600 was measured with an Infinite F200 microplate reader (Tecan).

    Techniques: Lysis, Plasmid Preparation, Planar Chromatography, Concentration Assay

    Lysis dynamics of TOP10 bearing the thermoinducible lysis device in low copy plasmid grown in microplate reader . OD600 of TOP10 with pLC-T4LysHeat induced with a temperature shift from 30°C to 42°C in the microplate reader (blue line). Heat-induced pLC-T4Lys - (green line) is shown as the negative control. Induction was performed in exponential phase at OD600 = 0.3. Error bars represent the 95% confidence interval of the estimated mean. For clarity of presentation, data points shown here are resampled with a 30-minute sampling time.

    Journal: Journal of Biological Engineering

    Article Title: Characterization of a synthetic bacterial self-destruction device for programmed cell death and for recombinant proteins release

    doi: 10.1186/1754-1611-5-8

    Figure Lengend Snippet: Lysis dynamics of TOP10 bearing the thermoinducible lysis device in low copy plasmid grown in microplate reader . OD600 of TOP10 with pLC-T4LysHeat induced with a temperature shift from 30°C to 42°C in the microplate reader (blue line). Heat-induced pLC-T4Lys - (green line) is shown as the negative control. Induction was performed in exponential phase at OD600 = 0.3. Error bars represent the 95% confidence interval of the estimated mean. For clarity of presentation, data points shown here are resampled with a 30-minute sampling time.

    Article Snippet: After that time, a 200 μl aliquot of each culture was transferred in a flat-bottomed 96-well microplate (Greiner) and the OD600 was measured with an Infinite F200 microplate reader (Tecan).

    Techniques: Lysis, Plasmid Preparation, Planar Chromatography, Negative Control, Sampling

    S. epidermidis attachment to polystyrene surfaces in the presence or absence of DNase I . (A) Attached SE1457 ΔatlE , SE1457 ΔsaeRS , SE1457 and SE1457 saec cells were observed by microscopy. Briefly, cell suspensions from the mid-exponential phase were diluted to OD600 = 0.1 in PBS and then incubated in wells (1 mL per well) of cell-culture polystyrene chambers (Nunc, Roskilde, Denmark) with DNase I (140 U/mL) for 2 h at 37°C. S. epidermidis cells attached to the polystyrene surface were counted under microscope (400× magnification). (B) The number of attached bacteria per field was then counted. Results represent the mean ± SD of three independent experiments. *, P

    Journal: BMC Microbiology

    Article Title: Role of the SaeRS two-component regulatory system in Staphylococcus epidermidis autolysis and biofilm formation

    doi: 10.1186/1471-2180-11-146

    Figure Lengend Snippet: S. epidermidis attachment to polystyrene surfaces in the presence or absence of DNase I . (A) Attached SE1457 ΔatlE , SE1457 ΔsaeRS , SE1457 and SE1457 saec cells were observed by microscopy. Briefly, cell suspensions from the mid-exponential phase were diluted to OD600 = 0.1 in PBS and then incubated in wells (1 mL per well) of cell-culture polystyrene chambers (Nunc, Roskilde, Denmark) with DNase I (140 U/mL) for 2 h at 37°C. S. epidermidis cells attached to the polystyrene surface were counted under microscope (400× magnification). (B) The number of attached bacteria per field was then counted. Results represent the mean ± SD of three independent experiments. *, P

    Article Snippet: The OD600 of the cultures was measured at 60 min intervals for 12 h. For anaerobic growth conditions, bacteria were cultured in the Eppendorf tubes that were filled up with the TSB medium and sealed with wax.

    Techniques: Microscopy, Incubation, Cell Culture

    Effect of saeRS deletion on Triton X-100-induced autolysis . SE1457 ΔsaeRS , SE1457, and SE1457 saec cells were diluted in TSB medium containing 1 M NaCl, grown to mid-exponential phase (OD600 = ~0.6-0.8), and resuspended in the same volume of 0.05 M Tris-HCl solution containing 0.05% Triton X-100 (pH 7.2). OD600 readings were measured every 30 min. The autolysis rate induced by Triton X-100 was calculated as follows: lysis rate = OD0 - ODt/OD0. The experiments were carried out in triplicate independently. WT, SE1457; SAE, SE1457 ΔsaeRS ; SAEC, SE1457 saec.

    Journal: BMC Microbiology

    Article Title: Role of the SaeRS two-component regulatory system in Staphylococcus epidermidis autolysis and biofilm formation

    doi: 10.1186/1471-2180-11-146

    Figure Lengend Snippet: Effect of saeRS deletion on Triton X-100-induced autolysis . SE1457 ΔsaeRS , SE1457, and SE1457 saec cells were diluted in TSB medium containing 1 M NaCl, grown to mid-exponential phase (OD600 = ~0.6-0.8), and resuspended in the same volume of 0.05 M Tris-HCl solution containing 0.05% Triton X-100 (pH 7.2). OD600 readings were measured every 30 min. The autolysis rate induced by Triton X-100 was calculated as follows: lysis rate = OD0 - ODt/OD0. The experiments were carried out in triplicate independently. WT, SE1457; SAE, SE1457 ΔsaeRS ; SAEC, SE1457 saec.

    Article Snippet: The OD600 of the cultures was measured at 60 min intervals for 12 h. For anaerobic growth conditions, bacteria were cultured in the Eppendorf tubes that were filled up with the TSB medium and sealed with wax.

    Techniques: Lysis

    Quantification of eDNA in SE1457 ΔsaeRS , SE1457, and SE1457 saec biofilms . eDNA was extracted from the unwashed 24 h biofilms of SE1457 ΔsaeRS (white bars), SE1457 (black bars), and SE1457 saec (gray bars). The eDNA in each biofilm was quantified by qPCR using primers specific for gyrA, serp0306, lysA , and leuA [ 19 , 28 ]. The quantity of eDNA was calculated as follows: total eDNA (ng)/relative OD600. Results represent the mean ± SD of three independent experiments. WT, SE1457; SAE, SE1457 ΔsaeRS ; SAEC, SE1457 saec.

    Journal: BMC Microbiology

    Article Title: Role of the SaeRS two-component regulatory system in Staphylococcus epidermidis autolysis and biofilm formation

    doi: 10.1186/1471-2180-11-146

    Figure Lengend Snippet: Quantification of eDNA in SE1457 ΔsaeRS , SE1457, and SE1457 saec biofilms . eDNA was extracted from the unwashed 24 h biofilms of SE1457 ΔsaeRS (white bars), SE1457 (black bars), and SE1457 saec (gray bars). The eDNA in each biofilm was quantified by qPCR using primers specific for gyrA, serp0306, lysA , and leuA [ 19 , 28 ]. The quantity of eDNA was calculated as follows: total eDNA (ng)/relative OD600. Results represent the mean ± SD of three independent experiments. WT, SE1457; SAE, SE1457 ΔsaeRS ; SAEC, SE1457 saec.

    Article Snippet: The OD600 of the cultures was measured at 60 min intervals for 12 h. For anaerobic growth conditions, bacteria were cultured in the Eppendorf tubes that were filled up with the TSB medium and sealed with wax.

    Techniques: Real-time Polymerase Chain Reaction

    Growth curves of P. fluorescens strains cLP6a and cLP6a-1 . Growth of P. fluorescens strains cLP6a and cLP6a-1 at (a) 10°C, (b) 28°C or (c) 35°C determined as OD600 Each data point is the mean of three independent cultures, and error bars, where visible, indicate the standard deviation.

    Journal: BMC Microbiology

    Article Title: An alternative physiological role for the EmhABC efflux pump in Pseudomonas fluorescens cLP6a

    doi: 10.1186/1471-2180-11-252

    Figure Lengend Snippet: Growth curves of P. fluorescens strains cLP6a and cLP6a-1 . Growth of P. fluorescens strains cLP6a and cLP6a-1 at (a) 10°C, (b) 28°C or (c) 35°C determined as OD600 Each data point is the mean of three independent cultures, and error bars, where visible, indicate the standard deviation.

    Article Snippet: Growth was measured as optical density at 600 nm (OD600 ) using an Ultrospec 3100 pro UV/Visible spectrophotometer (GE Healthcare Bio-Sciences), diluting the TSB blank and culture sample with distilled water as necessary.

    Techniques: Standard Deviation