octasaccharides Search Results


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  • 93
    Millipore pasteurella multocida
    Pasteurella Multocida, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher surveyor hplc system
    Surveyor Hplc System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1380 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Durect Corporation micro osmotic pump
    Micro Osmotic Pump, supplied by Durect Corporation, used in various techniques. Bioz Stars score: 91/100, based on 308 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Millipore naoh
    Naoh, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 7059 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore bovine testicular hyaluronidase
    Bovine Testicular Hyaluronidase, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 664 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    R&D Systems fgf10
    <t>Fgf10-induced</t> lacrimal gland budding was abolished in the Ugdh mutants. ( A-F ) In E13.5 control explant culture, BSA-containing beads did not perturb budding of the endogenous lacrimal gland (A, arrow), whereas Fgf10-containing beads induced ectopic lacrimal
    Fgf10, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 488 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Millipore hexasaccharide
    ( A ) Binding preferences of mIL-7 to various chain lengths of heparin using a SPR competition assay. Heparin disaccharide (dp2), tetrasaccharide (dp4), <t>hexasaccharide</t> (dp6), and octasaccharide (dp8) were each pre-equilibrated at a concentration of 1,000
    Hexasaccharide, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Millipore piperidine
    ( A ) Binding preferences of mIL-7 to various chain lengths of heparin using a SPR competition assay. Heparin disaccharide (dp2), tetrasaccharide (dp4), <t>hexasaccharide</t> (dp6), and octasaccharide (dp8) were each pre-equilibrated at a concentration of 1,000
    Piperidine, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 405 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher semi preparative scale carbopac pa1 column
    ( A ) Binding preferences of mIL-7 to various chain lengths of heparin using a SPR competition assay. Heparin disaccharide (dp2), tetrasaccharide (dp4), <t>hexasaccharide</t> (dp6), and octasaccharide (dp8) were each pre-equilibrated at a concentration of 1,000
    Semi Preparative Scale Carbopac Pa1 Column, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Elicityl lnno
    CLL subset 4 IGs do not bind i or I carbohydrate antigens. (A) Glycan sequences of the lipid-linked oligosaccharides <t>pLNH,</t> pLNnH, <t>LNnO</t> and I-octa that were among 76 additional (i- and Fc10.2- antigen-negative probes) in the microarray. Abbreviations: Gal, galactose; GlcNAc, N-acetylglucosamine; Glc, glucose; DH, L-1,2-dihexadecyl- sn -glycero-3-phosphoethanolamine; Cer, ceramide. (B), (C) Microarray analysis of CLL mAbs 141, 282, 183, 240, and 342, in either (B) native bivalent or (C) precomplexed condition. The human anti-i IgM PIA ELL, human anti-I Ma, and the murine IgM Fc10.2 were included as positive controls. The results are the means of fluorescence intensities of duplicate spots, printed at 5 fmol, with error bars representing half the difference between the two values.
    Lnno, supplied by Elicityl, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher 500 ion chromatography system
    CLL subset 4 IGs do not bind i or I carbohydrate antigens. (A) Glycan sequences of the lipid-linked oligosaccharides <t>pLNH,</t> pLNnH, <t>LNnO</t> and I-octa that were among 76 additional (i- and Fc10.2- antigen-negative probes) in the microarray. Abbreviations: Gal, galactose; GlcNAc, N-acetylglucosamine; Glc, glucose; DH, L-1,2-dihexadecyl- sn -glycero-3-phosphoethanolamine; Cer, ceramide. (B), (C) Microarray analysis of CLL mAbs 141, 282, 183, 240, and 342, in either (B) native bivalent or (C) precomplexed condition. The human anti-i IgM PIA ELL, human anti-I Ma, and the murine IgM Fc10.2 were included as positive controls. The results are the means of fluorescence intensities of duplicate spots, printed at 5 fmol, with error bars representing half the difference between the two values.
    500 Ion Chromatography System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Millipore tetrasaccharide
    CLL subset 4 IGs do not bind i or I carbohydrate antigens. (A) Glycan sequences of the lipid-linked oligosaccharides <t>pLNH,</t> pLNnH, <t>LNnO</t> and I-octa that were among 76 additional (i- and Fc10.2- antigen-negative probes) in the microarray. Abbreviations: Gal, galactose; GlcNAc, N-acetylglucosamine; Glc, glucose; DH, L-1,2-dihexadecyl- sn -glycero-3-phosphoethanolamine; Cer, ceramide. (B), (C) Microarray analysis of CLL mAbs 141, 282, 183, 240, and 342, in either (B) native bivalent or (C) precomplexed condition. The human anti-i IgM PIA ELL, human anti-I Ma, and the murine IgM Fc10.2 were included as positive controls. The results are the means of fluorescence intensities of duplicate spots, printed at 5 fmol, with error bars representing half the difference between the two values.
    Tetrasaccharide, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Millipore bovine lung heparin
    CLL subset 4 IGs do not bind i or I carbohydrate antigens. (A) Glycan sequences of the lipid-linked oligosaccharides <t>pLNH,</t> pLNnH, <t>LNnO</t> and I-octa that were among 76 additional (i- and Fc10.2- antigen-negative probes) in the microarray. Abbreviations: Gal, galactose; GlcNAc, N-acetylglucosamine; Glc, glucose; DH, L-1,2-dihexadecyl- sn -glycero-3-phosphoethanolamine; Cer, ceramide. (B), (C) Microarray analysis of CLL mAbs 141, 282, 183, 240, and 342, in either (B) native bivalent or (C) precomplexed condition. The human anti-i IgM PIA ELL, human anti-I Ma, and the murine IgM Fc10.2 were included as positive controls. The results are the means of fluorescence intensities of duplicate spots, printed at 5 fmol, with error bars representing half the difference between the two values.
    Bovine Lung Heparin, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher saxs analysis
    CLL subset 4 IGs do not bind i or I carbohydrate antigens. (A) Glycan sequences of the lipid-linked oligosaccharides <t>pLNH,</t> pLNnH, <t>LNnO</t> and I-octa that were among 76 additional (i- and Fc10.2- antigen-negative probes) in the microarray. Abbreviations: Gal, galactose; GlcNAc, N-acetylglucosamine; Glc, glucose; DH, L-1,2-dihexadecyl- sn -glycero-3-phosphoethanolamine; Cer, ceramide. (B), (C) Microarray analysis of CLL mAbs 141, 282, 183, 240, and 342, in either (B) native bivalent or (C) precomplexed condition. The human anti-i IgM PIA ELL, human anti-I Ma, and the murine IgM Fc10.2 were included as positive controls. The results are the means of fluorescence intensities of duplicate spots, printed at 5 fmol, with error bars representing half the difference between the two values.
    Saxs Analysis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Medicago rhizobium meliloti
    Western blot showing ExoR protein profiles of the S. <t>meliloti</t> wild-type Rm1021, the exoR95 mutant, the exoR108-chvI109 double mutant, and the exoR108-chvI109 (pHC510) double mutant, which is labeled as a p exoR mutant expressing the wild-type exoR gene.
    Rhizobium Meliloti, supplied by Medicago, used in various techniques. Bioz Stars score: 91/100, based on 233 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Megazyme xyloglucan derived oligosaccharides
    Western blot showing ExoR protein profiles of the S. <t>meliloti</t> wild-type Rm1021, the exoR95 mutant, the exoR108-chvI109 double mutant, and the exoR108-chvI109 (pHC510) double mutant, which is labeled as a p exoR mutant expressing the wild-type exoR gene.
    Xyloglucan Derived Oligosaccharides, supplied by Megazyme, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    The Nest Group dispo biodialyzers
    Western blot showing ExoR protein profiles of the S. <t>meliloti</t> wild-type Rm1021, the exoR95 mutant, the exoR108-chvI109 double mutant, and the exoR108-chvI109 (pHC510) double mutant, which is labeled as a p exoR mutant expressing the wild-type exoR gene.
    Dispo Biodialyzers, supplied by The Nest Group, used in various techniques. Bioz Stars score: 85/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Malvern Panalytical malvern peaq itc microcalorimeter
    Western blot showing ExoR protein profiles of the S. <t>meliloti</t> wild-type Rm1021, the exoR95 mutant, the exoR108-chvI109 double mutant, and the exoR108-chvI109 (pHC510) double mutant, which is labeled as a p exoR mutant expressing the wild-type exoR gene.
    Malvern Peaq Itc Microcalorimeter, supplied by Malvern Panalytical, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Millipore bovine kidney
    Western blot showing ExoR protein profiles of the S. <t>meliloti</t> wild-type Rm1021, the exoR95 mutant, the exoR108-chvI109 double mutant, and the exoR108-chvI109 (pHC510) double mutant, which is labeled as a p exoR mutant expressing the wild-type exoR gene.
    Bovine Kidney, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 243 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Biacore heparan sulfate
    A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and <t>heparan</t> sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,
    Heparan Sulfate, supplied by Biacore, used in various techniques. Bioz Stars score: 90/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Mimetics heparin mimetics
    A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and <t>heparan</t> sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,
    Heparin Mimetics, supplied by Mimetics, used in various techniques. Bioz Stars score: 91/100, based on 534 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    SLIT2 LTD robo1 binding hs structures
    SPR sensorgram representing the concentration-dependent kinetic analysis of the binding of 1 with immobilized <t>Robo1</t> on a CM5 chip. Concentration of compound 1 (from top to bottom): 100, 80, 40, 20, 10, 5, and 2.5 μ M, fitted with a Langmuir 1:1
    Robo1 Binding Hs Structures, supplied by SLIT2 LTD, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Waters Corporation c18 sep pak cartridge
    SPR sensorgram representing the concentration-dependent kinetic analysis of the binding of 1 with immobilized <t>Robo1</t> on a CM5 chip. Concentration of compound 1 (from top to bottom): 100, 80, 40, 20, 10, 5, and 2.5 μ M, fitted with a Langmuir 1:1
    C18 Sep Pak Cartridge, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 93/100, based on 1073 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    APP Pharmaceuticals heparin
    SPR sensorgram representing the concentration-dependent kinetic analysis of the binding of 1 with immobilized <t>Robo1</t> on a CM5 chip. Concentration of compound 1 (from top to bottom): 100, 80, 40, 20, 10, 5, and 2.5 μ M, fitted with a Langmuir 1:1
    Heparin, supplied by APP Pharmaceuticals, used in various techniques. Bioz Stars score: 92/100, based on 223 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Millipore cellotetraose
    SPR sensorgram representing the concentration-dependent kinetic analysis of the binding of 1 with immobilized <t>Robo1</t> on a CM5 chip. Concentration of compound 1 (from top to bottom): 100, 80, 40, 20, 10, 5, and 2.5 μ M, fitted with a Langmuir 1:1
    Cellotetraose, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Fgf10-induced lacrimal gland budding was abolished in the Ugdh mutants. ( A-F ) In E13.5 control explant culture, BSA-containing beads did not perturb budding of the endogenous lacrimal gland (A, arrow), whereas Fgf10-containing beads induced ectopic lacrimal

    Journal: Development (Cambridge, England)

    Article Title: Glycosaminoglycan-dependent restriction of FGF diffusion is necessary for lacrimal gland development

    doi: 10.1242/dev.079236

    Figure Lengend Snippet: Fgf10-induced lacrimal gland budding was abolished in the Ugdh mutants. ( A-F ) In E13.5 control explant culture, BSA-containing beads did not perturb budding of the endogenous lacrimal gland (A, arrow), whereas Fgf10-containing beads induced ectopic lacrimal

    Article Snippet: Ashikari-Hada and colleagues have previously reported that Fgf10 requires 6- O but not 2- O sulfate groups for interaction with octasaccharides in vitro ( ).

    Techniques:

    Ugdh deletion abolished GAG synthesis. ( A-H ) At E10.5, the Ugdh CKO mutants lost CS and HS (3G10) staining in the mesenchyme (A,B,E,F, arrows). There was little HS 10E4 staining in the mesenchyme at this stage (C,G), but the LACE staining of Fgf10/Fgfr2b,

    Journal: Development (Cambridge, England)

    Article Title: Glycosaminoglycan-dependent restriction of FGF diffusion is necessary for lacrimal gland development

    doi: 10.1242/dev.079236

    Figure Lengend Snippet: Ugdh deletion abolished GAG synthesis. ( A-H ) At E10.5, the Ugdh CKO mutants lost CS and HS (3G10) staining in the mesenchyme (A,B,E,F, arrows). There was little HS 10E4 staining in the mesenchyme at this stage (C,G), but the LACE staining of Fgf10/Fgfr2b,

    Article Snippet: Ashikari-Hada and colleagues have previously reported that Fgf10 requires 6- O but not 2- O sulfate groups for interaction with octasaccharides in vitro ( ).

    Techniques: Staining

    The Ugdh mutants preserved the mesenchymal Fgf10 expression but lost the epithelial FGF signaling in lacrimal gland development. ( A-J ) RNA in situ hybridization showed that the periocular markers Crabp1, FoxC1 and Pitx2 were unaffected in E10.5 and E12.5

    Journal: Development (Cambridge, England)

    Article Title: Glycosaminoglycan-dependent restriction of FGF diffusion is necessary for lacrimal gland development

    doi: 10.1242/dev.079236

    Figure Lengend Snippet: The Ugdh mutants preserved the mesenchymal Fgf10 expression but lost the epithelial FGF signaling in lacrimal gland development. ( A-J ) RNA in situ hybridization showed that the periocular markers Crabp1, FoxC1 and Pitx2 were unaffected in E10.5 and E12.5

    Article Snippet: Ashikari-Hada and colleagues have previously reported that Fgf10 requires 6- O but not 2- O sulfate groups for interaction with octasaccharides in vitro ( ).

    Techniques: Expressing, RNA In Situ Hybridization

    Expanded Fgf10 diffusion in the Ugdh mutants. ( A , B ) Western blots of E13.5 periocular mesenchyme showed that the Fgf10 protein was expressed at similar levels in control and Ugdh CKO mutants. After normalization using ERK proteins in the same lysates,

    Journal: Development (Cambridge, England)

    Article Title: Glycosaminoglycan-dependent restriction of FGF diffusion is necessary for lacrimal gland development

    doi: 10.1242/dev.079236

    Figure Lengend Snippet: Expanded Fgf10 diffusion in the Ugdh mutants. ( A , B ) Western blots of E13.5 periocular mesenchyme showed that the Fgf10 protein was expressed at similar levels in control and Ugdh CKO mutants. After normalization using ERK proteins in the same lysates,

    Article Snippet: Ashikari-Hada and colleagues have previously reported that Fgf10 requires 6- O but not 2- O sulfate groups for interaction with octasaccharides in vitro ( ).

    Techniques: Diffusion-based Assay, Western Blot

    Mesenchymal ablation of Ndst1/2 , but not Hs6st/Hs2st , disrupted lacrimal gland budding. ( A-H ) Mesenchymal deletion of Ndst1 ( Wnt1-Cre; Ndst1 flox/flox or Ndst1 CKO ) disrupted both HS 10E4 staining and the Fgf10/Fgfr2b LACE signal, but lacrimal gland budding

    Journal: Development (Cambridge, England)

    Article Title: Glycosaminoglycan-dependent restriction of FGF diffusion is necessary for lacrimal gland development

    doi: 10.1242/dev.079236

    Figure Lengend Snippet: Mesenchymal ablation of Ndst1/2 , but not Hs6st/Hs2st , disrupted lacrimal gland budding. ( A-H ) Mesenchymal deletion of Ndst1 ( Wnt1-Cre; Ndst1 flox/flox or Ndst1 CKO ) disrupted both HS 10E4 staining and the Fgf10/Fgfr2b LACE signal, but lacrimal gland budding

    Article Snippet: Ashikari-Hada and colleagues have previously reported that Fgf10 requires 6- O but not 2- O sulfate groups for interaction with octasaccharides in vitro ( ).

    Techniques: Staining

    ( A ) Binding preferences of mIL-7 to various chain lengths of heparin using a SPR competition assay. Heparin disaccharide (dp2), tetrasaccharide (dp4), hexasaccharide (dp6), and octasaccharide (dp8) were each pre-equilibrated at a concentration of 1,000

    Journal: Biochimie

    Article Title: Biophysical Characterization of Glycosaminoglycan-IL-7 Interactions using SPR

    doi: 10.1016/j.biochi.2011.10.015

    Figure Lengend Snippet: ( A ) Binding preferences of mIL-7 to various chain lengths of heparin using a SPR competition assay. Heparin disaccharide (dp2), tetrasaccharide (dp4), hexasaccharide (dp6), and octasaccharide (dp8) were each pre-equilibrated at a concentration of 1,000

    Article Snippet: A disaccharide (dp2), tetrasaccharide (dp4), hexasaccharide (dp6), and octasaccharide (dp8) form of heparin was prepared from controlled partial heparin lyase 1 treatment of bovine lung heparin (Sigma) [ ].

    Techniques: Binding Assay, SPR Assay, Competitive Binding Assay, Concentration Assay

    ( A ) Binding preferences of human IL-7 to various chain lengths of heparin using a SPR competition assay. A disaccharide (dp2), a tetrasaccharide (dp4), a hexasaccharide (dp6), and an octasaccarharide (dp8) of heparin was pre-equilibrated at a concentration

    Journal: Biochimie

    Article Title: Biophysical Characterization of Glycosaminoglycan-IL-7 Interactions using SPR

    doi: 10.1016/j.biochi.2011.10.015

    Figure Lengend Snippet: ( A ) Binding preferences of human IL-7 to various chain lengths of heparin using a SPR competition assay. A disaccharide (dp2), a tetrasaccharide (dp4), a hexasaccharide (dp6), and an octasaccarharide (dp8) of heparin was pre-equilibrated at a concentration

    Article Snippet: A disaccharide (dp2), tetrasaccharide (dp4), hexasaccharide (dp6), and octasaccharide (dp8) form of heparin was prepared from controlled partial heparin lyase 1 treatment of bovine lung heparin (Sigma) [ ].

    Techniques: Binding Assay, SPR Assay, Competitive Binding Assay, Concentration Assay

    CLL subset 4 IGs do not bind i or I carbohydrate antigens. (A) Glycan sequences of the lipid-linked oligosaccharides pLNH, pLNnH, LNnO and I-octa that were among 76 additional (i- and Fc10.2- antigen-negative probes) in the microarray. Abbreviations: Gal, galactose; GlcNAc, N-acetylglucosamine; Glc, glucose; DH, L-1,2-dihexadecyl- sn -glycero-3-phosphoethanolamine; Cer, ceramide. (B), (C) Microarray analysis of CLL mAbs 141, 282, 183, 240, and 342, in either (B) native bivalent or (C) precomplexed condition. The human anti-i IgM PIA ELL, human anti-I Ma, and the murine IgM Fc10.2 were included as positive controls. The results are the means of fluorescence intensities of duplicate spots, printed at 5 fmol, with error bars representing half the difference between the two values.

    Journal: Molecular Medicine

    Article Title: Binding of CLL Subset 4 B Cell Receptor Immunoglobulins to Viable Human Memory B Lymphocytes Requires a Distinctive IGKV Somatic Mutation

    doi: 10.2119/molmed.2017.00003

    Figure Lengend Snippet: CLL subset 4 IGs do not bind i or I carbohydrate antigens. (A) Glycan sequences of the lipid-linked oligosaccharides pLNH, pLNnH, LNnO and I-octa that were among 76 additional (i- and Fc10.2- antigen-negative probes) in the microarray. Abbreviations: Gal, galactose; GlcNAc, N-acetylglucosamine; Glc, glucose; DH, L-1,2-dihexadecyl- sn -glycero-3-phosphoethanolamine; Cer, ceramide. (B), (C) Microarray analysis of CLL mAbs 141, 282, 183, 240, and 342, in either (B) native bivalent or (C) precomplexed condition. The human anti-i IgM PIA ELL, human anti-I Ma, and the murine IgM Fc10.2 were included as positive controls. The results are the means of fluorescence intensities of duplicate spots, printed at 5 fmol, with error bars representing half the difference between the two values.

    Article Snippet: Two hexasaccharides, pLNH and pLNnH, from Dextra and an octasaccharide, LNnO, from Elicityl were analyzed as lipid-linked glycan probes, neoglycolipids (NGLs) derived by conjugation to a phospholipid L-1,2-dihexadecyl- sn -glycero-3-phosphoethanolamine (DHPE) ( ).

    Techniques: Microarray, Gas Chromatography, Fluorescence

    Western blot showing ExoR protein profiles of the S. meliloti wild-type Rm1021, the exoR95 mutant, the exoR108-chvI109 double mutant, and the exoR108-chvI109 (pHC510) double mutant, which is labeled as a p exoR mutant expressing the wild-type exoR gene.

    Journal: Journal of Bacteriology

    Article Title: Sinorhizobium meliloti ExoR Is the Target of Periplasmic Proteolysis

    doi: 10.1128/JB.00313-12

    Figure Lengend Snippet: Western blot showing ExoR protein profiles of the S. meliloti wild-type Rm1021, the exoR95 mutant, the exoR108-chvI109 double mutant, and the exoR108-chvI109 (pHC510) double mutant, which is labeled as a p exoR mutant expressing the wild-type exoR gene.

    Article Snippet: Biosynthetic control of molecular weight in the polymerization of the octasaccharide subunits of succinoglycan, a symbiotically important exopolysaccharide of Rhizobium meliloti .

    Techniques: Western Blot, Mutagenesis, Labeling, Expressing

    Cellular location of ExoR protein and site of proteolysis. (A and B) Schematic representation of three different ExoR-PhoA fusions (A) and their corresponding phosphatase activities in the S. meliloti Rm8002 background (B). The vertically hatched boxes

    Journal: Journal of Bacteriology

    Article Title: Sinorhizobium meliloti ExoR Is the Target of Periplasmic Proteolysis

    doi: 10.1128/JB.00313-12

    Figure Lengend Snippet: Cellular location of ExoR protein and site of proteolysis. (A and B) Schematic representation of three different ExoR-PhoA fusions (A) and their corresponding phosphatase activities in the S. meliloti Rm8002 background (B). The vertically hatched boxes

    Article Snippet: Biosynthetic control of molecular weight in the polymerization of the octasaccharide subunits of succinoglycan, a symbiotically important exopolysaccharide of Rhizobium meliloti .

    Techniques:

    Amino acid sequence alignment of ExoR proteolysis regions from 16 ExoR orthologs. Conserved amino acids are shaded. The overall amino acid identities between S. meliloti ExoR and its orthologs are listed. The positions of the amino acids and the site

    Journal: Journal of Bacteriology

    Article Title: Sinorhizobium meliloti ExoR Is the Target of Periplasmic Proteolysis

    doi: 10.1128/JB.00313-12

    Figure Lengend Snippet: Amino acid sequence alignment of ExoR proteolysis regions from 16 ExoR orthologs. Conserved amino acids are shaded. The overall amino acid identities between S. meliloti ExoR and its orthologs are listed. The positions of the amino acids and the site

    Article Snippet: Biosynthetic control of molecular weight in the polymerization of the octasaccharide subunits of succinoglycan, a symbiotically important exopolysaccharide of Rhizobium meliloti .

    Techniques: Sequencing

    A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,

    Journal:

    Article Title: Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *

    doi: 10.1074/jbc.M109.002840

    Figure Lengend Snippet: A , SPR binding assays. Shown is the injection of full-length α5β1 integrin (512 n m ) over immobilized heparin (145 resonance units, solid line ) and heparan sulfate ( dashed line ) (2 m m MgCl 2 , 0. 1 m m CaCl 2 , 15 μl/min; contact time,

    Article Snippet: The inhibition of α5β1 integrin binding to heparin and heparan sulfate increased with the length of oligosaccharides and then reached a plateau.

    Techniques: SPR Assay, Binding Assay, Injection

    Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin

    Journal:

    Article Title: Molecular Interplay between Endostatin, Integrins, and Heparan Sulfate *

    doi: 10.1074/jbc.M109.002840

    Figure Lengend Snippet: Heparin/Heparan Sulfate Bind to the Extracellular Domain of α5β1 Integrin

    Article Snippet: The inhibition of α5β1 integrin binding to heparin and heparan sulfate increased with the length of oligosaccharides and then reached a plateau.

    Techniques:

    SPR sensorgram representing the concentration-dependent kinetic analysis of the binding of 1 with immobilized Robo1 on a CM5 chip. Concentration of compound 1 (from top to bottom): 100, 80, 40, 20, 10, 5, and 2.5 μ M, fitted with a Langmuir 1:1

    Journal: Journal of the American Chemical Society

    Article Title: Integrated Approach to Identify Heparan Sulfate Ligand Requirements of Robo1

    doi: 10.1021/jacs.6b08161

    Figure Lengend Snippet: SPR sensorgram representing the concentration-dependent kinetic analysis of the binding of 1 with immobilized Robo1 on a CM5 chip. Concentration of compound 1 (from top to bottom): 100, 80, 40, 20, 10, 5, and 2.5 μ M, fitted with a Langmuir 1:1

    Article Snippet: To determine if the deduced Robo1 binding HS structures modulate Slit2–Robo1 signaling in a cellular context, the Robo1-binding octasaccharide ( 1 ), the Robo1-binding tetrasaccharide IdoA-GlcNS6S-IdoA-GlcNS6S ( 19 ), and the non-Robo1-binding tetrasaccharide IdoA-GlcNS6S-IdoA2S-GlcNS6S ( 20 ) were tested by cosupplementing with Slit2 in the lower chamber in the assay ( ).

    Techniques: SPR Assay, Concentration Assay, Binding Assay, Chromatin Immunoprecipitation

    Robo1-binding octa- and tetrasaccharides reverted the inhibition of Slit2–Robo1 signaling on mouse diaphragm endothelial cell migration. Serum-starved immortalized mouse lung endothelial cells in serum-free medium were seeded into the upper chamber

    Journal: Journal of the American Chemical Society

    Article Title: Integrated Approach to Identify Heparan Sulfate Ligand Requirements of Robo1

    doi: 10.1021/jacs.6b08161

    Figure Lengend Snippet: Robo1-binding octa- and tetrasaccharides reverted the inhibition of Slit2–Robo1 signaling on mouse diaphragm endothelial cell migration. Serum-starved immortalized mouse lung endothelial cells in serum-free medium were seeded into the upper chamber

    Article Snippet: To determine if the deduced Robo1 binding HS structures modulate Slit2–Robo1 signaling in a cellular context, the Robo1-binding octasaccharide ( 1 ), the Robo1-binding tetrasaccharide IdoA-GlcNS6S-IdoA-GlcNS6S ( 19 ), and the non-Robo1-binding tetrasaccharide IdoA-GlcNS6S-IdoA2S-GlcNS6S ( 20 ) were tested by cosupplementing with Slit2 in the lower chamber in the assay ( ).

    Techniques: Binding Assay, Inhibition, Migration

    Composition analysis of the octasaccharide mixture, the GFP-bound fraction, and the Robo1-bound fraction. Each composition is given as follows: [ΔHexA, HexA, GlcN, Ac, SO 3 ]. Abundances are relative to the overall amount of HS detected in that

    Journal: Journal of the American Chemical Society

    Article Title: Integrated Approach to Identify Heparan Sulfate Ligand Requirements of Robo1

    doi: 10.1021/jacs.6b08161

    Figure Lengend Snippet: Composition analysis of the octasaccharide mixture, the GFP-bound fraction, and the Robo1-bound fraction. Each composition is given as follows: [ΔHexA, HexA, GlcN, Ac, SO 3 ]. Abundances are relative to the overall amount of HS detected in that

    Article Snippet: To determine if the deduced Robo1 binding HS structures modulate Slit2–Robo1 signaling in a cellular context, the Robo1-binding octasaccharide ( 1 ), the Robo1-binding tetrasaccharide IdoA-GlcNS6S-IdoA-GlcNS6S ( 19 ), and the non-Robo1-binding tetrasaccharide IdoA-GlcNS6S-IdoA2S-GlcNS6S ( 20 ) were tested by cosupplementing with Slit2 in the lower chamber in the assay ( ).

    Techniques: