Journal: Scientific Reports
Article Title: β-Oxidation in ghrelin-producing cells is important for ghrelin acyl-modification
Figure Lengend Snippet: Effect of inhibiting β-oxidation on ghrelin production in PG-1 cells. PG-1 cells were plated in 24-well plates and etomoxir (ET, 5 µmol/L), octanoate (OA, 50 µmol/L), or a mixture of stearate and palmitate (LA, 50 µmol/L) was added. After incubation, acyl-ghrelin levels (a) , des-acyl ghrelin levels (b) , and acyl/total ghrelin ratio (c) in the medium were measured. Des-acyl ghrelin levels were not different between groups. ET treatment decreased acyl-ghrelin levels and the acyl/total ghrelin ratio, and ET + OA rescued acyl-ghrelin and acyl/total ghrelin ratio (a–c) . On the other hand, Ghrl and Mboat4 mRNA expression was not changed between all experimental groups (d , e) . PG-1 cell morphology did not change after the etomoxir treatment (f) . This experiment was replicated twice and the results of 2nd data set were used in this figure. Scale bars=500 μm Each value represents the mean ± SEM. n = 3.
Article Snippet: Simultaneously, sodium octanoate (C5038, Sigma-Aldrich) or mixture of sodium stearate and palmitate (199–03285, Wako) was added at a final concentration of 50 µmol/L and cells were incubated in a 37 °C incubator with 5% CO2 for 24 hours.
Techniques: Incubation, Expressing