Journal: Biochemistry

Article Title: Molecular Mechanism of Photoactivation and Structural Location of the Cyanobacterial Orange Carotenoid Protein

doi: 10.1021/bi401539w

Figure Lengend Snippet: The structural location of the OCP in PBS. (A) The OCP (cyan) binds to the basal cylinder containing both APC 660 trimers and APC 680 . ApcA (light blue), ApcB (wheat), ApcE (raserry). 3D structure of ApcE phycocyanobilin binding domain (raspberry) was predicted

Article Snippet: To separate the resulting PBS-OCP complexes from the unbound OCP after illumination, the sample was loaded onto a 100 kDa filter (Millipore Amicon Centrifugal Filters, Billerica, MA) and centrifuged at 4,000×g at 23 °C for 20 min. Fresh 0.8 M phosphate buffer was applied three times to dilute the retentate.

Techniques: Binding Assay

Journal: Analytical and Bioanalytical Chemistry

Article Title: Analytical methods for PCBs and organochlorine pesticides in environmental monitoring and surveillance: a critical appraisal

doi: 10.1007/s00216-006-0765-y

Figure Lengend Snippet: Illustration of the basic components of an ELISA for detection of OCPs and PCBs in environmental samples or extracts. Sample antigen (analyte) competes with antigen for binding sites on coating protein; after a wash step, detection is performed by adding substrate and chromophore

Article Snippet: Commercial ELISA kits for detection of PCBs and most OCPs are available from Millipore Corp. (Billerica, MA, USA) and Strategic Diagnostics (Newark, DE, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Environmental Sampling, Binding Assay

Journal: Solid State Nuclear Magnetic Resonance

Article Title: Solid state NMR - An indispensable tool in organic-inorganic biocomposite characterization; refining the structure of octacalcium phosphate composites with the linear metabolic di-acids succinate and adipate

doi: 10.1016/j.ssnmr.2018.08.004

Figure Lengend Snippet: 13 C— 13 C proton-driven spin diffusion correlation (PDSD, 5 ms mixing time) experiments on uniformly 13 C labelled OCP-SUC (left) and OCP-ADI (right). Correlations proving a single non-centrosymmetric ADI within the OCP-ADI structure are highlighted in red. Corresponding 1D 13 C spectra are superimposed. Signal broadening relative to unlabelled composites is ascribed to residual 13 C— 13 C dipolar interactions, and unresolved 13 C— 13 C scalar coupling. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: 2.1 Synthesis of OCP, OCP-SUC, and OCP-ADI Most reagents were purchased from Sigma-Aldrich and used as received. (U-13 C4 )-SUC and (U—13 C6 )-ADI were purchased from Cambridge Isotope Laboratories (Andover, MS, USA).

Techniques: Diffusion-based Assay, Mass Spectrometry

Journal: Solid State Nuclear Magnetic Resonance

Article Title: Solid state NMR - An indispensable tool in organic-inorganic biocomposite characterization; refining the structure of octacalcium phosphate composites with the linear metabolic di-acids succinate and adipate

doi: 10.1016/j.ssnmr.2018.08.004

Figure Lengend Snippet: Diagrams on the right depict structures of OCP-SUC (top) and OCP-ADI (bottom) consistent with two identical non-centrosymmetric di-acids per unit cell, modified from those implied by Model I of Markovic et al. [ 18 ], (left) which implies the existence of two identical and centrosymmetric di-acids.

Article Snippet: 2.1 Synthesis of OCP, OCP-SUC, and OCP-ADI Most reagents were purchased from Sigma-Aldrich and used as received. (U-13 C4 )-SUC and (U—13 C6 )-ADI were purchased from Cambridge Isotope Laboratories (Andover, MS, USA).

Techniques: Modification

Journal: Solid State Nuclear Magnetic Resonance

Article Title: Solid state NMR - An indispensable tool in organic-inorganic biocomposite characterization; refining the structure of octacalcium phosphate composites with the linear metabolic di-acids succinate and adipate

doi: 10.1016/j.ssnmr.2018.08.004

Figure Lengend Snippet: Typical 13 C{ 31 P} REDOR data (top) for OCP-SUC (left) and OCP-ADI (right), and resultant dephasing curves (bottom), showing the non-equivalence of the carboxylate carbons, and of the methylene carbons, with respect to neighbourhood phosphorus atoms Error bars in the dephasing curves represent spectral signal-to-noise measurements at each respective dephasing time.

Article Snippet: 2.1 Synthesis of OCP, OCP-SUC, and OCP-ADI Most reagents were purchased from Sigma-Aldrich and used as received. (U-13 C4 )-SUC and (U—13 C6 )-ADI were purchased from Cambridge Isotope Laboratories (Andover, MS, USA).

Techniques:

Journal: Solid State Nuclear Magnetic Resonance

Article Title: Solid state NMR - An indispensable tool in organic-inorganic biocomposite characterization; refining the structure of octacalcium phosphate composites with the linear metabolic di-acids succinate and adipate

doi: 10.1016/j.ssnmr.2018.08.004

Figure Lengend Snippet: Comparison between 13 C, and 31 P, NMR spectra of different batches of OCP-SUC and OCP-ADI, and pure OCP. Minor 13 C signals in some batches, of variable occurrence, are marked by asterisks.

Article Snippet: 2.1 Synthesis of OCP, OCP-SUC, and OCP-ADI Most reagents were purchased from Sigma-Aldrich and used as received. (U-13 C4 )-SUC and (U—13 C6 )-ADI were purchased from Cambridge Isotope Laboratories (Andover, MS, USA).

Techniques: Nuclear Magnetic Resonance

Journal: Scientific Reports

Article Title: The photocycle of orange carotenoid protein conceals distinct intermediates and asynchronous changes in the carotenoid and protein components

doi: 10.1038/s41598-017-15520-4

Figure Lengend Snippet: ( A ) flash-induced transitions of OCP absorption at 550 nm approximated by multiexponential decay: OCP – red, OCP in 1 M phosphate – grey; OCP in the presence of FRP (1/1.6 concentration ratio) – blue; ΔNTE OCP in the presence of FRP (1/1.6 concentration ratio) – black. Note the logarithmic timescale covering almost 7 orders of magnitude. ( B ) dependency of the photoconversion amplitude on the energy of the laser flash for OCP in the presence of FRP (1/1.6 concentration ratio). ( C ) Arrhenius plot of the fast (C1) and the slow (C3) components’ rate constants of absorption changes of OCP in the presence of FRP. Experiments were conducted in the range of temperatures from 6 to 37 °C.

Article Snippet: Time-Correlated Single-Photon Counting (TCSPC) techniques for tryptophan fluorescence transients In all experiments, OCP photoconversion was triggered by a blue LED (M455L3, Thorlabs, USA), which passed through the FB450-40 bandpass filter (Thorlabs, USA).

Techniques: Concentration Assay