oadc enrichment Search Results


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  • 99
    Thermo Fisher remel oadc enrichment
    Remel Oadc Enrichment, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 7 article reviews
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    remel oadc enrichment - by Bioz Stars, 2020-04
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    99
    Millipore oadc growth supplement
    Oadc Growth Supplement, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Difco oadc enrichment
    Growth of M. bovis Δ nadABC and M. tuberculosis Δ nadABC in vitro. A . The strains were grown with NAm (20 mg/l) to log phase, spun down, washed 5 X with PBS, resuspended in media without NAm, and inoculated into media containing either NAm at different concentrations or NAc at 20 mg/l. The complemented strains were grown without any supplement. The cultures were incubated with shaking at 37°C for 3 weeks and growth was followed by measuring OD 600nm . B . The strains were grown, washed, diluted, and inoculated in media containing or not containing NAm (20 mg/l). At each time point, samples were taken, diluted, and plated onto <t>Middlebrook</t> 7H10 plates containing <t>OADC,</t> glycerol, and NAm (20 mg/l). The plates were incubated at 37°C for 4-5 weeks and colonies were counted. The concentrations are in mg/l.
    Oadc Enrichment, supplied by Difco, used in various techniques. Bioz Stars score: 93/100, based on 102 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Becton Dickinson oadc enrichment
    Deletion of MSMEG_1285 in Msmeg mc 2 155 impacts the cell-surface exposure of HBHA_ Mtb . AFM tomographic image (left panel), representative spatially-resolved map of adhesion forces recorded with AFM heparin-coated tip (medium panel) and corresponding histogram (right panel, obtained from 756 force curves) for ( A ) the wild-type strain of Msmeg , ( B ) Msmeg mc 2 155 expressing hbhA_Mtb , ( C ) the Δ MSMEG_1285 mutant and ( D ) the Δ MSMEG_1285 mutant expressing hbhA_Mtb . All the strains were cultured in <t>7H9</t> supplemented with <t>OADC</t> and without detergent. For each image, the red square corresponds to the area scanned for the determination of one adhesion map.
    Oadc Enrichment, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 93/100, based on 239 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Fisher Scientific oadc enrichment
    Deletion of MSMEG_1285 in Msmeg mc 2 155 impacts the cell-surface exposure of HBHA_ Mtb . AFM tomographic image (left panel), representative spatially-resolved map of adhesion forces recorded with AFM heparin-coated tip (medium panel) and corresponding histogram (right panel, obtained from 756 force curves) for ( A ) the wild-type strain of Msmeg , ( B ) Msmeg mc 2 155 expressing hbhA_Mtb , ( C ) the Δ MSMEG_1285 mutant and ( D ) the Δ MSMEG_1285 mutant expressing hbhA_Mtb . All the strains were cultured in <t>7H9</t> supplemented with <t>OADC</t> and without detergent. For each image, the red square corresponds to the area scanned for the determination of one adhesion map.
    Oadc Enrichment, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    MiddleBrook Pharmaceuticals oadc enrichment
    Deletion of MSMEG_1285 in Msmeg mc 2 155 impacts the cell-surface exposure of HBHA_ Mtb . AFM tomographic image (left panel), representative spatially-resolved map of adhesion forces recorded with AFM heparin-coated tip (medium panel) and corresponding histogram (right panel, obtained from 756 force curves) for ( A ) the wild-type strain of Msmeg , ( B ) Msmeg mc 2 155 expressing hbhA_Mtb , ( C ) the Δ MSMEG_1285 mutant and ( D ) the Δ MSMEG_1285 mutant expressing hbhA_Mtb . All the strains were cultured in <t>7H9</t> supplemented with <t>OADC</t> and without detergent. For each image, the red square corresponds to the area scanned for the determination of one adhesion map.
    Oadc Enrichment, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 94/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Becton Dickinson middlebrook oadc enrichment
    Deletion of MSMEG_1285 in Msmeg mc 2 155 impacts the cell-surface exposure of HBHA_ Mtb . AFM tomographic image (left panel), representative spatially-resolved map of adhesion forces recorded with AFM heparin-coated tip (medium panel) and corresponding histogram (right panel, obtained from 756 force curves) for ( A ) the wild-type strain of Msmeg , ( B ) Msmeg mc 2 155 expressing hbhA_Mtb , ( C ) the Δ MSMEG_1285 mutant and ( D ) the Δ MSMEG_1285 mutant expressing hbhA_Mtb . All the strains were cultured in <t>7H9</t> supplemented with <t>OADC</t> and without detergent. For each image, the red square corresponds to the area scanned for the determination of one adhesion map.
    Middlebrook Oadc Enrichment, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Difco middlebrook oadc enrichment
    Deletion of MSMEG_1285 in Msmeg mc 2 155 impacts the cell-surface exposure of HBHA_ Mtb . AFM tomographic image (left panel), representative spatially-resolved map of adhesion forces recorded with AFM heparin-coated tip (medium panel) and corresponding histogram (right panel, obtained from 756 force curves) for ( A ) the wild-type strain of Msmeg , ( B ) Msmeg mc 2 155 expressing hbhA_Mtb , ( C ) the Δ MSMEG_1285 mutant and ( D ) the Δ MSMEG_1285 mutant expressing hbhA_Mtb . All the strains were cultured in <t>7H9</t> supplemented with <t>OADC</t> and without detergent. For each image, the red square corresponds to the area scanned for the determination of one adhesion map.
    Middlebrook Oadc Enrichment, supplied by Difco, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Becton Dickinson oadc enrichment broth
    Deletion of MSMEG_1285 in Msmeg mc 2 155 impacts the cell-surface exposure of HBHA_ Mtb . AFM tomographic image (left panel), representative spatially-resolved map of adhesion forces recorded with AFM heparin-coated tip (medium panel) and corresponding histogram (right panel, obtained from 756 force curves) for ( A ) the wild-type strain of Msmeg , ( B ) Msmeg mc 2 155 expressing hbhA_Mtb , ( C ) the Δ MSMEG_1285 mutant and ( D ) the Δ MSMEG_1285 mutant expressing hbhA_Mtb . All the strains were cultured in <t>7H9</t> supplemented with <t>OADC</t> and without detergent. For each image, the red square corresponds to the area scanned for the determination of one adhesion map.
    Oadc Enrichment Broth, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 95/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Thermo Fisher middlebrook oadc enrichment
    Deletion of MSMEG_1285 in Msmeg mc 2 155 impacts the cell-surface exposure of HBHA_ Mtb . AFM tomographic image (left panel), representative spatially-resolved map of adhesion forces recorded with AFM heparin-coated tip (medium panel) and corresponding histogram (right panel, obtained from 756 force curves) for ( A ) the wild-type strain of Msmeg , ( B ) Msmeg mc 2 155 expressing hbhA_Mtb , ( C ) the Δ MSMEG_1285 mutant and ( D ) the Δ MSMEG_1285 mutant expressing hbhA_Mtb . All the strains were cultured in <t>7H9</t> supplemented with <t>OADC</t> and without detergent. For each image, the red square corresponds to the area scanned for the determination of one adhesion map.
    Middlebrook Oadc Enrichment, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Avantor middlebrook oadc enrichment
    Deletion of MSMEG_1285 in Msmeg mc 2 155 impacts the cell-surface exposure of HBHA_ Mtb . AFM tomographic image (left panel), representative spatially-resolved map of adhesion forces recorded with AFM heparin-coated tip (medium panel) and corresponding histogram (right panel, obtained from 756 force curves) for ( A ) the wild-type strain of Msmeg , ( B ) Msmeg mc 2 155 expressing hbhA_Mtb , ( C ) the Δ MSMEG_1285 mutant and ( D ) the Δ MSMEG_1285 mutant expressing hbhA_Mtb . All the strains were cultured in <t>7H9</t> supplemented with <t>OADC</t> and without detergent. For each image, the red square corresponds to the area scanned for the determination of one adhesion map.
    Middlebrook Oadc Enrichment, supplied by Avantor, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson oadc enrichment medium
    Deletion of MSMEG_1285 in Msmeg mc 2 155 impacts the cell-surface exposure of HBHA_ Mtb . AFM tomographic image (left panel), representative spatially-resolved map of adhesion forces recorded with AFM heparin-coated tip (medium panel) and corresponding histogram (right panel, obtained from 756 force curves) for ( A ) the wild-type strain of Msmeg , ( B ) Msmeg mc 2 155 expressing hbhA_Mtb , ( C ) the Δ MSMEG_1285 mutant and ( D ) the Δ MSMEG_1285 mutant expressing hbhA_Mtb . All the strains were cultured in <t>7H9</t> supplemented with <t>OADC</t> and without detergent. For each image, the red square corresponds to the area scanned for the determination of one adhesion map.
    Oadc Enrichment Medium, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 93/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    87
    Becton Dickinson oadc enrichment solution
    Deletion of MSMEG_1285 in Msmeg mc 2 155 impacts the cell-surface exposure of HBHA_ Mtb . AFM tomographic image (left panel), representative spatially-resolved map of adhesion forces recorded with AFM heparin-coated tip (medium panel) and corresponding histogram (right panel, obtained from 756 force curves) for ( A ) the wild-type strain of Msmeg , ( B ) Msmeg mc 2 155 expressing hbhA_Mtb , ( C ) the Δ MSMEG_1285 mutant and ( D ) the Δ MSMEG_1285 mutant expressing hbhA_Mtb . All the strains were cultured in <t>7H9</t> supplemented with <t>OADC</t> and without detergent. For each image, the red square corresponds to the area scanned for the determination of one adhesion map.
    Oadc Enrichment Solution, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 87/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Becton Dickinson oadc growth supplement enrichment
    Deletion of MSMEG_1285 in Msmeg mc 2 155 impacts the cell-surface exposure of HBHA_ Mtb . AFM tomographic image (left panel), representative spatially-resolved map of adhesion forces recorded with AFM heparin-coated tip (medium panel) and corresponding histogram (right panel, obtained from 756 force curves) for ( A ) the wild-type strain of Msmeg , ( B ) Msmeg mc 2 155 expressing hbhA_Mtb , ( C ) the Δ MSMEG_1285 mutant and ( D ) the Δ MSMEG_1285 mutant expressing hbhA_Mtb . All the strains were cultured in <t>7H9</t> supplemented with <t>OADC</t> and without detergent. For each image, the red square corresponds to the area scanned for the determination of one adhesion map.
    Oadc Growth Supplement Enrichment, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Thermo Fisher oadc enrichment medium
    Deletion of MSMEG_1285 in Msmeg mc 2 155 impacts the cell-surface exposure of HBHA_ Mtb . AFM tomographic image (left panel), representative spatially-resolved map of adhesion forces recorded with AFM heparin-coated tip (medium panel) and corresponding histogram (right panel, obtained from 756 force curves) for ( A ) the wild-type strain of Msmeg , ( B ) Msmeg mc 2 155 expressing hbhA_Mtb , ( C ) the Δ MSMEG_1285 mutant and ( D ) the Δ MSMEG_1285 mutant expressing hbhA_Mtb . All the strains were cultured in <t>7H9</t> supplemented with <t>OADC</t> and without detergent. For each image, the red square corresponds to the area scanned for the determination of one adhesion map.
    Oadc Enrichment Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Becton Dickinson catalase oadc enrichment
    Deletion of MSMEG_1285 in Msmeg mc 2 155 impacts the cell-surface exposure of HBHA_ Mtb . AFM tomographic image (left panel), representative spatially-resolved map of adhesion forces recorded with AFM heparin-coated tip (medium panel) and corresponding histogram (right panel, obtained from 756 force curves) for ( A ) the wild-type strain of Msmeg , ( B ) Msmeg mc 2 155 expressing hbhA_Mtb , ( C ) the Δ MSMEG_1285 mutant and ( D ) the Δ MSMEG_1285 mutant expressing hbhA_Mtb . All the strains were cultured in <t>7H9</t> supplemented with <t>OADC</t> and without detergent. For each image, the red square corresponds to the area scanned for the determination of one adhesion map.
    Catalase Oadc Enrichment, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Millipore oadc enrichment
    Deletion of MSMEG_1285 in Msmeg mc 2 155 impacts the cell-surface exposure of HBHA_ Mtb . AFM tomographic image (left panel), representative spatially-resolved map of adhesion forces recorded with AFM heparin-coated tip (medium panel) and corresponding histogram (right panel, obtained from 756 force curves) for ( A ) the wild-type strain of Msmeg , ( B ) Msmeg mc 2 155 expressing hbhA_Mtb , ( C ) the Δ MSMEG_1285 mutant and ( D ) the Δ MSMEG_1285 mutant expressing hbhA_Mtb . All the strains were cultured in <t>7H9</t> supplemented with <t>OADC</t> and without detergent. For each image, the red square corresponds to the area scanned for the determination of one adhesion map.
    Oadc Enrichment, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Becton Dickinson oadc enrichment media supplement
    Deletion of MSMEG_1285 in Msmeg mc 2 155 impacts the cell-surface exposure of HBHA_ Mtb . AFM tomographic image (left panel), representative spatially-resolved map of adhesion forces recorded with AFM heparin-coated tip (medium panel) and corresponding histogram (right panel, obtained from 756 force curves) for ( A ) the wild-type strain of Msmeg , ( B ) Msmeg mc 2 155 expressing hbhA_Mtb , ( C ) the Δ MSMEG_1285 mutant and ( D ) the Δ MSMEG_1285 mutant expressing hbhA_Mtb . All the strains were cultured in <t>7H9</t> supplemented with <t>OADC</t> and without detergent. For each image, the red square corresponds to the area scanned for the determination of one adhesion map.
    Oadc Enrichment Media Supplement, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 85/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Difco catalase oadc enrichment
    Deletion of MSMEG_1285 in Msmeg mc 2 155 impacts the cell-surface exposure of HBHA_ Mtb . AFM tomographic image (left panel), representative spatially-resolved map of adhesion forces recorded with AFM heparin-coated tip (medium panel) and corresponding histogram (right panel, obtained from 756 force curves) for ( A ) the wild-type strain of Msmeg , ( B ) Msmeg mc 2 155 expressing hbhA_Mtb , ( C ) the Δ MSMEG_1285 mutant and ( D ) the Δ MSMEG_1285 mutant expressing hbhA_Mtb . All the strains were cultured in <t>7H9</t> supplemented with <t>OADC</t> and without detergent. For each image, the red square corresponds to the area scanned for the determination of one adhesion map.
    Catalase Oadc Enrichment, supplied by Difco, used in various techniques. Bioz Stars score: 86/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    MiddleBrook Pharmaceuticals middlebrook oadc enrichment
    Survival of Mtb strains in the presence of DETA/NO or menadione. Mtb strains were cultured in 7H9 <t>Middlebrook</t> with 10% of <t>OADC,</t> with or without various concentrations of DETA/NO or menadione for 6 days. The number of viable bacteria was determined by the CFU method. Data are presented as percentage of Mtb survival inhibition, expressed as means ± SEM (n = 3).
    Middlebrook Oadc Enrichment, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Difco oadc enriched 7h10 agar medium
    Survival of Mtb strains in the presence of DETA/NO or menadione. Mtb strains were cultured in 7H9 <t>Middlebrook</t> with 10% of <t>OADC,</t> with or without various concentrations of DETA/NO or menadione for 6 days. The number of viable bacteria was determined by the CFU method. Data are presented as percentage of Mtb survival inhibition, expressed as means ± SEM (n = 3).
    Oadc Enriched 7h10 Agar Medium, supplied by Difco, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Difco oleic acid adc enrichment oadc
    Survival of Mtb strains in the presence of DETA/NO or menadione. Mtb strains were cultured in 7H9 <t>Middlebrook</t> with 10% of <t>OADC,</t> with or without various concentrations of DETA/NO or menadione for 6 days. The number of viable bacteria was determined by the CFU method. Data are presented as percentage of Mtb survival inhibition, expressed as means ± SEM (n = 3).
    Oleic Acid Adc Enrichment Oadc, supplied by Difco, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Difco oadc enriched mueller hinton agar
    Survival of Mtb strains in the presence of DETA/NO or menadione. Mtb strains were cultured in 7H9 <t>Middlebrook</t> with 10% of <t>OADC,</t> with or without various concentrations of DETA/NO or menadione for 6 days. The number of viable bacteria was determined by the CFU method. Data are presented as percentage of Mtb survival inhibition, expressed as means ± SEM (n = 3).
    Oadc Enriched Mueller Hinton Agar, supplied by Difco, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson oleic acid adc oadc enrichment
    Survival of Mtb strains in the presence of DETA/NO or menadione. Mtb strains were cultured in 7H9 <t>Middlebrook</t> with 10% of <t>OADC,</t> with or without various concentrations of DETA/NO or menadione for 6 days. The number of viable bacteria was determined by the CFU method. Data are presented as percentage of Mtb survival inhibition, expressed as means ± SEM (n = 3).
    Oleic Acid Adc Oadc Enrichment, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    MiddleBrook Pharmaceuticals oadc enrichment medium
    Survival of Mtb strains in the presence of DETA/NO or menadione. Mtb strains were cultured in 7H9 <t>Middlebrook</t> with 10% of <t>OADC,</t> with or without various concentrations of DETA/NO or menadione for 6 days. The number of viable bacteria was determined by the CFU method. Data are presented as percentage of Mtb survival inhibition, expressed as means ± SEM (n = 3).
    Oadc Enrichment Medium, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 92/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Hardy Diagnostics catalase oadc enrichment
    Survival of Mtb strains in the presence of DETA/NO or menadione. Mtb strains were cultured in 7H9 <t>Middlebrook</t> with 10% of <t>OADC,</t> with or without various concentrations of DETA/NO or menadione for 6 days. The number of viable bacteria was determined by the CFU method. Data are presented as percentage of Mtb survival inhibition, expressed as means ± SEM (n = 3).
    Catalase Oadc Enrichment, supplied by Hardy Diagnostics, used in various techniques. Bioz Stars score: 86/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Difco oadc enrichment medium
    Survival of Mtb strains in the presence of DETA/NO or menadione. Mtb strains were cultured in 7H9 <t>Middlebrook</t> with 10% of <t>OADC,</t> with or without various concentrations of DETA/NO or menadione for 6 days. The number of viable bacteria was determined by the CFU method. Data are presented as percentage of Mtb survival inhibition, expressed as means ± SEM (n = 3).
    Oadc Enrichment Medium, supplied by Difco, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Survival of Mtb strains in the presence of DETA/NO or menadione. Mtb strains were cultured in 7H9 <t>Middlebrook</t> with 10% of <t>OADC,</t> with or without various concentrations of DETA/NO or menadione for 6 days. The number of viable bacteria was determined by the CFU method. Data are presented as percentage of Mtb survival inhibition, expressed as means ± SEM (n = 3).
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    Survival of Mtb strains in the presence of DETA/NO or menadione. Mtb strains were cultured in 7H9 <t>Middlebrook</t> with 10% of <t>OADC,</t> with or without various concentrations of DETA/NO or menadione for 6 days. The number of viable bacteria was determined by the CFU method. Data are presented as percentage of Mtb survival inhibition, expressed as means ± SEM (n = 3).
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    Survival of Mtb strains in the presence of DETA/NO or menadione. Mtb strains were cultured in 7H9 <t>Middlebrook</t> with 10% of <t>OADC,</t> with or without various concentrations of DETA/NO or menadione for 6 days. The number of viable bacteria was determined by the CFU method. Data are presented as percentage of Mtb survival inhibition, expressed as means ± SEM (n = 3).
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    Image Search Results


    Growth of M. bovis Δ nadABC and M. tuberculosis Δ nadABC in vitro. A . The strains were grown with NAm (20 mg/l) to log phase, spun down, washed 5 X with PBS, resuspended in media without NAm, and inoculated into media containing either NAm at different concentrations or NAc at 20 mg/l. The complemented strains were grown without any supplement. The cultures were incubated with shaking at 37°C for 3 weeks and growth was followed by measuring OD 600nm . B . The strains were grown, washed, diluted, and inoculated in media containing or not containing NAm (20 mg/l). At each time point, samples were taken, diluted, and plated onto Middlebrook 7H10 plates containing OADC, glycerol, and NAm (20 mg/l). The plates were incubated at 37°C for 4-5 weeks and colonies were counted. The concentrations are in mg/l.

    Journal: Molecular microbiology

    Article Title: NAD+ Auxotrophy is Bacteriocidal for the Tubercle Bacilli

    doi: 10.1111/j.1365-2958.2010.07099.x

    Figure Lengend Snippet: Growth of M. bovis Δ nadABC and M. tuberculosis Δ nadABC in vitro. A . The strains were grown with NAm (20 mg/l) to log phase, spun down, washed 5 X with PBS, resuspended in media without NAm, and inoculated into media containing either NAm at different concentrations or NAc at 20 mg/l. The complemented strains were grown without any supplement. The cultures were incubated with shaking at 37°C for 3 weeks and growth was followed by measuring OD 600nm . B . The strains were grown, washed, diluted, and inoculated in media containing or not containing NAm (20 mg/l). At each time point, samples were taken, diluted, and plated onto Middlebrook 7H10 plates containing OADC, glycerol, and NAm (20 mg/l). The plates were incubated at 37°C for 4-5 weeks and colonies were counted. The concentrations are in mg/l.

    Article Snippet: The strains were grown in Middlebrook 7H9 medium (Difco, Sparks, MD) supplemented with 10% (v/v) OADC enrichment (Difco), 0.2% (v/v) glycerol, and 0.05% (v/v) tyloxapol.

    Techniques: In Vitro, Incubation

    Deletion of MSMEG_1285 in Msmeg mc 2 155 impacts the cell-surface exposure of HBHA_ Mtb . AFM tomographic image (left panel), representative spatially-resolved map of adhesion forces recorded with AFM heparin-coated tip (medium panel) and corresponding histogram (right panel, obtained from 756 force curves) for ( A ) the wild-type strain of Msmeg , ( B ) Msmeg mc 2 155 expressing hbhA_Mtb , ( C ) the Δ MSMEG_1285 mutant and ( D ) the Δ MSMEG_1285 mutant expressing hbhA_Mtb . All the strains were cultured in 7H9 supplemented with OADC and without detergent. For each image, the red square corresponds to the area scanned for the determination of one adhesion map.

    Journal: International Journal of Molecular Sciences

    Article Title: Rv0613c/MSMEG_1285 Interacts with HBHA and Mediates Its Proper Cell-Surface Exposure in Mycobacteria

    doi: 10.3390/ijms19061673

    Figure Lengend Snippet: Deletion of MSMEG_1285 in Msmeg mc 2 155 impacts the cell-surface exposure of HBHA_ Mtb . AFM tomographic image (left panel), representative spatially-resolved map of adhesion forces recorded with AFM heparin-coated tip (medium panel) and corresponding histogram (right panel, obtained from 756 force curves) for ( A ) the wild-type strain of Msmeg , ( B ) Msmeg mc 2 155 expressing hbhA_Mtb , ( C ) the Δ MSMEG_1285 mutant and ( D ) the Δ MSMEG_1285 mutant expressing hbhA_Mtb . All the strains were cultured in 7H9 supplemented with OADC and without detergent. For each image, the red square corresponds to the area scanned for the determination of one adhesion map.

    Article Snippet: Msmeg mc2 155, the deletion mutant Δ MSMEG_1285 and the corresponding complemented strain ( ) were grown in Sauton’s medium (containing 0.025% tyloxapol), in Middlebrook 7H9 broth or on 7H11 agar plates supplemented with OADC enrichment (Becton Dickinson, Le Pont-de-Claix, France), hygromycin (50 μg·mL−1 ) or kanamycin (25 μg·mL−1 ), when required.

    Techniques: Expressing, Mutagenesis, Cell Culture

    Heterologous expression of hbhA_Mtb in the Δ MSMEG_1285 mutant affects auto-aggregation and colony morphology. ( A ) Overnight cultures in 7H9 supplemented with OADC and without detergent of Msmeg mc 2 155 and the Δ MSMEG_1285 mutant, both expressing hbhA_Mtb ; ( B ) Serial dilution of Msmeg mc 2 155 (upper series) and the Δ MSMEG_1285 mutant (lower series), both expressing hbhA_Mtb , on 7H11 agar plates; ( C ) Western-blot analysis using monoclonal antibodies anti-Hsp65 (upper panel), anti-HBHA VF2 (medium panel) and anti-HBHA D2 (lower panel) on 40 µg of total lysates from Msmeg mc 2 155 and the Δ MSMEG_1285 mutant, both expressing hbhA_Mtb .

    Journal: International Journal of Molecular Sciences

    Article Title: Rv0613c/MSMEG_1285 Interacts with HBHA and Mediates Its Proper Cell-Surface Exposure in Mycobacteria

    doi: 10.3390/ijms19061673

    Figure Lengend Snippet: Heterologous expression of hbhA_Mtb in the Δ MSMEG_1285 mutant affects auto-aggregation and colony morphology. ( A ) Overnight cultures in 7H9 supplemented with OADC and without detergent of Msmeg mc 2 155 and the Δ MSMEG_1285 mutant, both expressing hbhA_Mtb ; ( B ) Serial dilution of Msmeg mc 2 155 (upper series) and the Δ MSMEG_1285 mutant (lower series), both expressing hbhA_Mtb , on 7H11 agar plates; ( C ) Western-blot analysis using monoclonal antibodies anti-Hsp65 (upper panel), anti-HBHA VF2 (medium panel) and anti-HBHA D2 (lower panel) on 40 µg of total lysates from Msmeg mc 2 155 and the Δ MSMEG_1285 mutant, both expressing hbhA_Mtb .

    Article Snippet: Msmeg mc2 155, the deletion mutant Δ MSMEG_1285 and the corresponding complemented strain ( ) were grown in Sauton’s medium (containing 0.025% tyloxapol), in Middlebrook 7H9 broth or on 7H11 agar plates supplemented with OADC enrichment (Becton Dickinson, Le Pont-de-Claix, France), hygromycin (50 μg·mL−1 ) or kanamycin (25 μg·mL−1 ), when required.

    Techniques: Expressing, Mutagenesis, Serial Dilution, Western Blot

    Analysis of the Δ D6 ;Δ D4 - attB:: P ami - D4 Msm conditional mutant phenotype. ( A ) Growth in 7H9/OADC medium with (solid line) and without (dashed line) acetamide was followed over time by measuring the OD 600 nm (top) and number of viable cells (bottom). Values are means ± standard errors from three independent experiments. ( B) (left) Images of the mutant liquid cultures after 24 h of growth with (+) and without (−) acetamide. (right) Mutant growth on 7H10/OADC agar with (+) and without (−) acetamide. ( C) (top) Monitoring of the accD4 Msm expression level. The accD4 Msm transcript level was measured in mutant strain growing for 12 and 24 h in 7H9/OADC medium with (+) and without (−) acetamide. The expression level of the same transcript was also measured in the ∆ accD6 parental strain after 24 h of culture. (bottom) Thin-layer chromatography of 14 C-labeled fatty acid methyl esters (FAMEs) and mycolic acid methyl esters (MAMEs) extracted from the mutant after 3, 12 and 24 h of culture in 7H9/OADC medium with (+) and without (−) acetamide. Equal counts (10 000 cpm) were loaded onto TLC plate and developed in hexane – ethyl acetate (19:1, vol/vol). The symbols: α, α′, and e correspond to α-mycolates, α′-mycolates and epoxy-mycolates, respectively.

    Journal: Scientific Reports

    Article Title: The influence of AccD5 on AccD6 carboxyltransferase essentiality in pathogenic and non-pathogenic Mycobacterium

    doi: 10.1038/srep42692

    Figure Lengend Snippet: Analysis of the Δ D6 ;Δ D4 - attB:: P ami - D4 Msm conditional mutant phenotype. ( A ) Growth in 7H9/OADC medium with (solid line) and without (dashed line) acetamide was followed over time by measuring the OD 600 nm (top) and number of viable cells (bottom). Values are means ± standard errors from three independent experiments. ( B) (left) Images of the mutant liquid cultures after 24 h of growth with (+) and without (−) acetamide. (right) Mutant growth on 7H10/OADC agar with (+) and without (−) acetamide. ( C) (top) Monitoring of the accD4 Msm expression level. The accD4 Msm transcript level was measured in mutant strain growing for 12 and 24 h in 7H9/OADC medium with (+) and without (−) acetamide. The expression level of the same transcript was also measured in the ∆ accD6 parental strain after 24 h of culture. (bottom) Thin-layer chromatography of 14 C-labeled fatty acid methyl esters (FAMEs) and mycolic acid methyl esters (MAMEs) extracted from the mutant after 3, 12 and 24 h of culture in 7H9/OADC medium with (+) and without (−) acetamide. Equal counts (10 000 cpm) were loaded onto TLC plate and developed in hexane – ethyl acetate (19:1, vol/vol). The symbols: α, α′, and e correspond to α-mycolates, α′-mycolates and epoxy-mycolates, respectively.

    Article Snippet: Strains based on M. tuberculosis and M. smegmatis were maintained on Middlebrook 7H10 agar or 7H9 broth (Becton Dickinson) with 10% OADC (oleic acid, albumin, dextrose, catalase) enrichment (Becton Dickinson).

    Techniques: Mutagenesis, Expressing, Thin Layer Chromatography, Labeling

    Survival of Mtb strains in the presence of DETA/NO or menadione. Mtb strains were cultured in 7H9 Middlebrook with 10% of OADC, with or without various concentrations of DETA/NO or menadione for 6 days. The number of viable bacteria was determined by the CFU method. Data are presented as percentage of Mtb survival inhibition, expressed as means ± SEM (n = 3).

    Journal: PLoS ONE

    Article Title: Either Non-Homologous Ends Joining or Homologous Recombination Is Required to Repair Double-Strand Breaks in the Genome of Macrophage-Internalized Mycobacterium tuberculosis

    doi: 10.1371/journal.pone.0092799

    Figure Lengend Snippet: Survival of Mtb strains in the presence of DETA/NO or menadione. Mtb strains were cultured in 7H9 Middlebrook with 10% of OADC, with or without various concentrations of DETA/NO or menadione for 6 days. The number of viable bacteria was determined by the CFU method. Data are presented as percentage of Mtb survival inhibition, expressed as means ± SEM (n = 3).

    Article Snippet: Middlebrook 7H10 agar, Middlebrook 7H9 broth, and Middlebrook OADC enrichment were acquired from Becton Dickinson (Franklin Lakes, NJ, USA).

    Techniques: Cell Culture, Inhibition

    Effect of inhibition of ROS and NO production on the survival of Mtb strains inside MØs. MØs were incubated with apocynin or L-NIL for 30 minutes, then infected with Mtb wild-type or Δ( ku,ligD,recA ) for 2 hours (MOI = 10) and washed with HBSS. On the day of infection and after 6 days of culture, MØs were lysed with Triton X-100 and cell lysates were plated onto Middlebrook 7H10 agar supplemented with 10% OADC. After 21 days of culture, CFUs were counted. Data are presented as fold increase in CFUs, expressed as means ± SEM (n = 5; #p≤0.04, Δ( ku,ligD,recA ) vs. Δ( ku,ligD,recA ) + apocynin or L-NIL; Mann-Whitney U test).

    Journal: PLoS ONE

    Article Title: Either Non-Homologous Ends Joining or Homologous Recombination Is Required to Repair Double-Strand Breaks in the Genome of Macrophage-Internalized Mycobacterium tuberculosis

    doi: 10.1371/journal.pone.0092799

    Figure Lengend Snippet: Effect of inhibition of ROS and NO production on the survival of Mtb strains inside MØs. MØs were incubated with apocynin or L-NIL for 30 minutes, then infected with Mtb wild-type or Δ( ku,ligD,recA ) for 2 hours (MOI = 10) and washed with HBSS. On the day of infection and after 6 days of culture, MØs were lysed with Triton X-100 and cell lysates were plated onto Middlebrook 7H10 agar supplemented with 10% OADC. After 21 days of culture, CFUs were counted. Data are presented as fold increase in CFUs, expressed as means ± SEM (n = 5; #p≤0.04, Δ( ku,ligD,recA ) vs. Δ( ku,ligD,recA ) + apocynin or L-NIL; Mann-Whitney U test).

    Article Snippet: Middlebrook 7H10 agar, Middlebrook 7H9 broth, and Middlebrook OADC enrichment were acquired from Becton Dickinson (Franklin Lakes, NJ, USA).

    Techniques: Inhibition, Incubation, Infection, MANN-WHITNEY

    Survival of Mtb strains inside MØs. (A) MØs were infected with Mtb wild-type or its mutants, Δ( ku,ligD,recA ), Δ( ku,ligD ) or Δ recA , for 2 hours (MOI = 10), washed with HBSS, and cultured for 2, 4, and 6 days. (B and C) MØs were infected with Mtb wild-type or its mutants (B) or complemented strains Δ( ku,ligD,recA )- P recA recA – complemented strain I or Δ( ku,ligD,recA )- P kuligD ku-ligD – complemented strain II (C) for 6 days. On the day of infection and after 6 days of culture, MØs were lysed with Triton X-100 and cell lysates were plated onto Middlebrook 7H10 agar supplemented with 10% OADC. After 21 days of culture, CFUs were counted. Data are presented as fold increase in CFUs, expressed as means ± SEM (n = 5; *p≤0.05, Δ( ku,ligD,recA ) vs. wild-type; #p≤0.05, complemented strain I and II vs. Δ( ku,ligD,recA ) Mann-Whitney U test).

    Journal: PLoS ONE

    Article Title: Either Non-Homologous Ends Joining or Homologous Recombination Is Required to Repair Double-Strand Breaks in the Genome of Macrophage-Internalized Mycobacterium tuberculosis

    doi: 10.1371/journal.pone.0092799

    Figure Lengend Snippet: Survival of Mtb strains inside MØs. (A) MØs were infected with Mtb wild-type or its mutants, Δ( ku,ligD,recA ), Δ( ku,ligD ) or Δ recA , for 2 hours (MOI = 10), washed with HBSS, and cultured for 2, 4, and 6 days. (B and C) MØs were infected with Mtb wild-type or its mutants (B) or complemented strains Δ( ku,ligD,recA )- P recA recA – complemented strain I or Δ( ku,ligD,recA )- P kuligD ku-ligD – complemented strain II (C) for 6 days. On the day of infection and after 6 days of culture, MØs were lysed with Triton X-100 and cell lysates were plated onto Middlebrook 7H10 agar supplemented with 10% OADC. After 21 days of culture, CFUs were counted. Data are presented as fold increase in CFUs, expressed as means ± SEM (n = 5; *p≤0.05, Δ( ku,ligD,recA ) vs. wild-type; #p≤0.05, complemented strain I and II vs. Δ( ku,ligD,recA ) Mann-Whitney U test).

    Article Snippet: Middlebrook 7H10 agar, Middlebrook 7H9 broth, and Middlebrook OADC enrichment were acquired from Becton Dickinson (Franklin Lakes, NJ, USA).

    Techniques: Infection, Cell Culture, MANN-WHITNEY