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  • 99
    Vector Laboratories m o m kit
    M O M Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1783 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    m o m kit - by Bioz Stars, 2021-01
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    Thermo Fisher h2 o
    H2 O, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 8635 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Cambridge Isotope Laboratories d2 o
    D2 O, supplied by Cambridge Isotope Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 3723 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    d2 o - by Bioz Stars, 2021-01
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    Takeda takeuchi o
    Takeuchi O, supplied by Takeda, used in various techniques. Bioz Stars score: 92/100, based on 2540 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    takeuchi o - by Bioz Stars, 2021-01
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    Sakura Finetek tissue tek o c t compound
    Tissue Tek O C T Compound, supplied by Sakura Finetek, used in various techniques. Bioz Stars score: 92/100, based on 3201 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore o phenylenediamine
    O Phenylenediamine, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2787 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Sakura Finetek o c t compound
    O C T Compound, supplied by Sakura Finetek, used in various techniques. Bioz Stars score: 92/100, based on 2777 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore o phenylenediamine dihydrochloride
    O Phenylenediamine Dihydrochloride, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2261 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore safranin o
    Elevated active TGF-β levels in human HO. a H E staining of normal muscle (left) and HO in early osteogenesis stage (middle) and late osteogenesis stage (right) after elbow fracture (EF) and central nervous system trauma (CNST). Scale bar, 100 μm. b <t>Safranin</t> O and Fast Green (SOFG) staining of normal muscle (left) and HO. Proteoglycan (red) and heterotopic bone (green). Scale bar, 100 μm. c TRAP + cells (red) and d quantitative analysis of the number of TRAP + osteoclast surface (OCS) per bone surface (BS). Scale bar, 100 μm. Immunohistochemical staining of e pSmad2/3 + cells (brown) and g PDGF-BB + cells (brown) and quantitative analysis of the number of f pSmad2/3 + cells or h PDGF-BB + cells per bone marrow area (mm 2 ). i , j Quantitative analysis of i active TGF-β and j PDGF-BB in serum in HO patients at early osteogenesis stage and late mature stage by ELISA. Dotted line indicates the average concentration of healthy people with active TGF-β of 1.23 ng/ml and PDGF-BB of 1.68 ng/ml. k Immunofluorescent staining and l quantitative analysis of CD73 + (green) and CD90 + cells (red) in bone marrow of ectopic bone marrow in HO patients. Blue color indicates DAPI staining of nuclei. Scale bar, 20 μm. C cartilage, B bone, BM bone marrow, M muscle, NM normal muscle, EF elbow fracture, CNST central nervous system trauma, OR osteogenesis MR maturation. All data are shown as the mean ± s.d. n = 9 per group for d , f , h , l histomorphometry analysis. * p
    Safranin O, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1387 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Dwyer Instruments o dwyer pj
    Elevated active TGF-β levels in human HO. a H E staining of normal muscle (left) and HO in early osteogenesis stage (middle) and late osteogenesis stage (right) after elbow fracture (EF) and central nervous system trauma (CNST). Scale bar, 100 μm. b <t>Safranin</t> O and Fast Green (SOFG) staining of normal muscle (left) and HO. Proteoglycan (red) and heterotopic bone (green). Scale bar, 100 μm. c TRAP + cells (red) and d quantitative analysis of the number of TRAP + osteoclast surface (OCS) per bone surface (BS). Scale bar, 100 μm. Immunohistochemical staining of e pSmad2/3 + cells (brown) and g PDGF-BB + cells (brown) and quantitative analysis of the number of f pSmad2/3 + cells or h PDGF-BB + cells per bone marrow area (mm 2 ). i , j Quantitative analysis of i active TGF-β and j PDGF-BB in serum in HO patients at early osteogenesis stage and late mature stage by ELISA. Dotted line indicates the average concentration of healthy people with active TGF-β of 1.23 ng/ml and PDGF-BB of 1.68 ng/ml. k Immunofluorescent staining and l quantitative analysis of CD73 + (green) and CD90 + cells (red) in bone marrow of ectopic bone marrow in HO patients. Blue color indicates DAPI staining of nuclei. Scale bar, 20 μm. C cartilage, B bone, BM bone marrow, M muscle, NM normal muscle, EF elbow fracture, CNST central nervous system trauma, OR osteogenesis MR maturation. All data are shown as the mean ± s.d. n = 9 per group for d , f , h , l histomorphometry analysis. * p
    O Dwyer Pj, supplied by Dwyer Instruments, used in various techniques. Bioz Stars score: 93/100, based on 512 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore toluidine blue
    Elevated active TGF-β levels in human HO. a H E staining of normal muscle (left) and HO in early osteogenesis stage (middle) and late osteogenesis stage (right) after elbow fracture (EF) and central nervous system trauma (CNST). Scale bar, 100 μm. b <t>Safranin</t> O and Fast Green (SOFG) staining of normal muscle (left) and HO. Proteoglycan (red) and heterotopic bone (green). Scale bar, 100 μm. c TRAP + cells (red) and d quantitative analysis of the number of TRAP + osteoclast surface (OCS) per bone surface (BS). Scale bar, 100 μm. Immunohistochemical staining of e pSmad2/3 + cells (brown) and g PDGF-BB + cells (brown) and quantitative analysis of the number of f pSmad2/3 + cells or h PDGF-BB + cells per bone marrow area (mm 2 ). i , j Quantitative analysis of i active TGF-β and j PDGF-BB in serum in HO patients at early osteogenesis stage and late mature stage by ELISA. Dotted line indicates the average concentration of healthy people with active TGF-β of 1.23 ng/ml and PDGF-BB of 1.68 ng/ml. k Immunofluorescent staining and l quantitative analysis of CD73 + (green) and CD90 + cells (red) in bone marrow of ectopic bone marrow in HO patients. Blue color indicates DAPI staining of nuclei. Scale bar, 20 μm. C cartilage, B bone, BM bone marrow, M muscle, NM normal muscle, EF elbow fracture, CNST central nervous system trauma, OR osteogenesis MR maturation. All data are shown as the mean ± s.d. n = 9 per group for d , f , h , l histomorphometry analysis. * p
    Toluidine Blue, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3843 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Millipore dianisidine dihydrochloride
    Elevated active TGF-β levels in human HO. a H E staining of normal muscle (left) and HO in early osteogenesis stage (middle) and late osteogenesis stage (right) after elbow fracture (EF) and central nervous system trauma (CNST). Scale bar, 100 μm. b <t>Safranin</t> O and Fast Green (SOFG) staining of normal muscle (left) and HO. Proteoglycan (red) and heterotopic bone (green). Scale bar, 100 μm. c TRAP + cells (red) and d quantitative analysis of the number of TRAP + osteoclast surface (OCS) per bone surface (BS). Scale bar, 100 μm. Immunohistochemical staining of e pSmad2/3 + cells (brown) and g PDGF-BB + cells (brown) and quantitative analysis of the number of f pSmad2/3 + cells or h PDGF-BB + cells per bone marrow area (mm 2 ). i , j Quantitative analysis of i active TGF-β and j PDGF-BB in serum in HO patients at early osteogenesis stage and late mature stage by ELISA. Dotted line indicates the average concentration of healthy people with active TGF-β of 1.23 ng/ml and PDGF-BB of 1.68 ng/ml. k Immunofluorescent staining and l quantitative analysis of CD73 + (green) and CD90 + cells (red) in bone marrow of ectopic bone marrow in HO patients. Blue color indicates DAPI staining of nuclei. Scale bar, 20 μm. C cartilage, B bone, BM bone marrow, M muscle, NM normal muscle, EF elbow fracture, CNST central nervous system trauma, OR osteogenesis MR maturation. All data are shown as the mean ± s.d. n = 9 per group for d , f , h , l histomorphometry analysis. * p
    Dianisidine Dihydrochloride, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 82 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore o dianisidine dihydrochloride
    Elevated active TGF-β levels in human HO. a H E staining of normal muscle (left) and HO in early osteogenesis stage (middle) and late osteogenesis stage (right) after elbow fracture (EF) and central nervous system trauma (CNST). Scale bar, 100 μm. b <t>Safranin</t> O and Fast Green (SOFG) staining of normal muscle (left) and HO. Proteoglycan (red) and heterotopic bone (green). Scale bar, 100 μm. c TRAP + cells (red) and d quantitative analysis of the number of TRAP + osteoclast surface (OCS) per bone surface (BS). Scale bar, 100 μm. Immunohistochemical staining of e pSmad2/3 + cells (brown) and g PDGF-BB + cells (brown) and quantitative analysis of the number of f pSmad2/3 + cells or h PDGF-BB + cells per bone marrow area (mm 2 ). i , j Quantitative analysis of i active TGF-β and j PDGF-BB in serum in HO patients at early osteogenesis stage and late mature stage by ELISA. Dotted line indicates the average concentration of healthy people with active TGF-β of 1.23 ng/ml and PDGF-BB of 1.68 ng/ml. k Immunofluorescent staining and l quantitative analysis of CD73 + (green) and CD90 + cells (red) in bone marrow of ectopic bone marrow in HO patients. Blue color indicates DAPI staining of nuclei. Scale bar, 20 μm. C cartilage, B bone, BM bone marrow, M muscle, NM normal muscle, EF elbow fracture, CNST central nervous system trauma, OR osteogenesis MR maturation. All data are shown as the mean ± s.d. n = 9 per group for d , f , h , l histomorphometry analysis. * p
    O Dianisidine Dihydrochloride, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1003 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Sakura Finetek tissue tek o c t
    Elevated active TGF-β levels in human HO. a H E staining of normal muscle (left) and HO in early osteogenesis stage (middle) and late osteogenesis stage (right) after elbow fracture (EF) and central nervous system trauma (CNST). Scale bar, 100 μm. b <t>Safranin</t> O and Fast Green (SOFG) staining of normal muscle (left) and HO. Proteoglycan (red) and heterotopic bone (green). Scale bar, 100 μm. c TRAP + cells (red) and d quantitative analysis of the number of TRAP + osteoclast surface (OCS) per bone surface (BS). Scale bar, 100 μm. Immunohistochemical staining of e pSmad2/3 + cells (brown) and g PDGF-BB + cells (brown) and quantitative analysis of the number of f pSmad2/3 + cells or h PDGF-BB + cells per bone marrow area (mm 2 ). i , j Quantitative analysis of i active TGF-β and j PDGF-BB in serum in HO patients at early osteogenesis stage and late mature stage by ELISA. Dotted line indicates the average concentration of healthy people with active TGF-β of 1.23 ng/ml and PDGF-BB of 1.68 ng/ml. k Immunofluorescent staining and l quantitative analysis of CD73 + (green) and CD90 + cells (red) in bone marrow of ectopic bone marrow in HO patients. Blue color indicates DAPI staining of nuclei. Scale bar, 20 μm. C cartilage, B bone, BM bone marrow, M muscle, NM normal muscle, EF elbow fracture, CNST central nervous system trauma, OR osteogenesis MR maturation. All data are shown as the mean ± s.d. n = 9 per group for d , f , h , l histomorphometry analysis. * p
    Tissue Tek O C T, supplied by Sakura Finetek, used in various techniques. Bioz Stars score: 92/100, based on 1012 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    O. Kindler kedo o
    Elevated active TGF-β levels in human HO. a H E staining of normal muscle (left) and HO in early osteogenesis stage (middle) and late osteogenesis stage (right) after elbow fracture (EF) and central nervous system trauma (CNST). Scale bar, 100 μm. b <t>Safranin</t> O and Fast Green (SOFG) staining of normal muscle (left) and HO. Proteoglycan (red) and heterotopic bone (green). Scale bar, 100 μm. c TRAP + cells (red) and d quantitative analysis of the number of TRAP + osteoclast surface (OCS) per bone surface (BS). Scale bar, 100 μm. Immunohistochemical staining of e pSmad2/3 + cells (brown) and g PDGF-BB + cells (brown) and quantitative analysis of the number of f pSmad2/3 + cells or h PDGF-BB + cells per bone marrow area (mm 2 ). i , j Quantitative analysis of i active TGF-β and j PDGF-BB in serum in HO patients at early osteogenesis stage and late mature stage by ELISA. Dotted line indicates the average concentration of healthy people with active TGF-β of 1.23 ng/ml and PDGF-BB of 1.68 ng/ml. k Immunofluorescent staining and l quantitative analysis of CD73 + (green) and CD90 + cells (red) in bone marrow of ectopic bone marrow in HO patients. Blue color indicates DAPI staining of nuclei. Scale bar, 20 μm. C cartilage, B bone, BM bone marrow, M muscle, NM normal muscle, EF elbow fracture, CNST central nervous system trauma, OR osteogenesis MR maturation. All data are shown as the mean ± s.d. n = 9 per group for d , f , h , l histomorphometry analysis. * p
    Kedo O, supplied by O. Kindler, used in various techniques. Bioz Stars score: 91/100, based on 221 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Cambridge Isotope Laboratories h2 o
    Elevated active TGF-β levels in human HO. a H E staining of normal muscle (left) and HO in early osteogenesis stage (middle) and late osteogenesis stage (right) after elbow fracture (EF) and central nervous system trauma (CNST). Scale bar, 100 μm. b <t>Safranin</t> O and Fast Green (SOFG) staining of normal muscle (left) and HO. Proteoglycan (red) and heterotopic bone (green). Scale bar, 100 μm. c TRAP + cells (red) and d quantitative analysis of the number of TRAP + osteoclast surface (OCS) per bone surface (BS). Scale bar, 100 μm. Immunohistochemical staining of e pSmad2/3 + cells (brown) and g PDGF-BB + cells (brown) and quantitative analysis of the number of f pSmad2/3 + cells or h PDGF-BB + cells per bone marrow area (mm 2 ). i , j Quantitative analysis of i active TGF-β and j PDGF-BB in serum in HO patients at early osteogenesis stage and late mature stage by ELISA. Dotted line indicates the average concentration of healthy people with active TGF-β of 1.23 ng/ml and PDGF-BB of 1.68 ng/ml. k Immunofluorescent staining and l quantitative analysis of CD73 + (green) and CD90 + cells (red) in bone marrow of ectopic bone marrow in HO patients. Blue color indicates DAPI staining of nuclei. Scale bar, 20 μm. C cartilage, B bone, BM bone marrow, M muscle, NM normal muscle, EF elbow fracture, CNST central nervous system trauma, OR osteogenesis MR maturation. All data are shown as the mean ± s.d. n = 9 per group for d , f , h , l histomorphometry analysis. * p
    H2 O, supplied by Cambridge Isotope Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 603 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Vector Laboratories biotinylated anti mouse
    Elevated active TGF-β levels in human HO. a H E staining of normal muscle (left) and HO in early osteogenesis stage (middle) and late osteogenesis stage (right) after elbow fracture (EF) and central nervous system trauma (CNST). Scale bar, 100 μm. b <t>Safranin</t> O and Fast Green (SOFG) staining of normal muscle (left) and HO. Proteoglycan (red) and heterotopic bone (green). Scale bar, 100 μm. c TRAP + cells (red) and d quantitative analysis of the number of TRAP + osteoclast surface (OCS) per bone surface (BS). Scale bar, 100 μm. Immunohistochemical staining of e pSmad2/3 + cells (brown) and g PDGF-BB + cells (brown) and quantitative analysis of the number of f pSmad2/3 + cells or h PDGF-BB + cells per bone marrow area (mm 2 ). i , j Quantitative analysis of i active TGF-β and j PDGF-BB in serum in HO patients at early osteogenesis stage and late mature stage by ELISA. Dotted line indicates the average concentration of healthy people with active TGF-β of 1.23 ng/ml and PDGF-BB of 1.68 ng/ml. k Immunofluorescent staining and l quantitative analysis of CD73 + (green) and CD90 + cells (red) in bone marrow of ectopic bone marrow in HO patients. Blue color indicates DAPI staining of nuclei. Scale bar, 20 μm. C cartilage, B bone, BM bone marrow, M muscle, NM normal muscle, EF elbow fracture, CNST central nervous system trauma, OR osteogenesis MR maturation. All data are shown as the mean ± s.d. n = 9 per group for d , f , h , l histomorphometry analysis. * p
    Biotinylated Anti Mouse, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1808 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore β mercaptoethanol
    Elevated active TGF-β levels in human HO. a H E staining of normal muscle (left) and HO in early osteogenesis stage (middle) and late osteogenesis stage (right) after elbow fracture (EF) and central nervous system trauma (CNST). Scale bar, 100 μm. b <t>Safranin</t> O and Fast Green (SOFG) staining of normal muscle (left) and HO. Proteoglycan (red) and heterotopic bone (green). Scale bar, 100 μm. c TRAP + cells (red) and d quantitative analysis of the number of TRAP + osteoclast surface (OCS) per bone surface (BS). Scale bar, 100 μm. Immunohistochemical staining of e pSmad2/3 + cells (brown) and g PDGF-BB + cells (brown) and quantitative analysis of the number of f pSmad2/3 + cells or h PDGF-BB + cells per bone marrow area (mm 2 ). i , j Quantitative analysis of i active TGF-β and j PDGF-BB in serum in HO patients at early osteogenesis stage and late mature stage by ELISA. Dotted line indicates the average concentration of healthy people with active TGF-β of 1.23 ng/ml and PDGF-BB of 1.68 ng/ml. k Immunofluorescent staining and l quantitative analysis of CD73 + (green) and CD90 + cells (red) in bone marrow of ectopic bone marrow in HO patients. Blue color indicates DAPI staining of nuclei. Scale bar, 20 μm. C cartilage, B bone, BM bone marrow, M muscle, NM normal muscle, EF elbow fracture, CNST central nervous system trauma, OR osteogenesis MR maturation. All data are shown as the mean ± s.d. n = 9 per group for d , f , h , l histomorphometry analysis. * p
    β Mercaptoethanol, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 28107 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore oil red o staining
    Elevated active TGF-β levels in human HO. a H E staining of normal muscle (left) and HO in early osteogenesis stage (middle) and late osteogenesis stage (right) after elbow fracture (EF) and central nervous system trauma (CNST). Scale bar, 100 μm. b <t>Safranin</t> O and Fast Green (SOFG) staining of normal muscle (left) and HO. Proteoglycan (red) and heterotopic bone (green). Scale bar, 100 μm. c TRAP + cells (red) and d quantitative analysis of the number of TRAP + osteoclast surface (OCS) per bone surface (BS). Scale bar, 100 μm. Immunohistochemical staining of e pSmad2/3 + cells (brown) and g PDGF-BB + cells (brown) and quantitative analysis of the number of f pSmad2/3 + cells or h PDGF-BB + cells per bone marrow area (mm 2 ). i , j Quantitative analysis of i active TGF-β and j PDGF-BB in serum in HO patients at early osteogenesis stage and late mature stage by ELISA. Dotted line indicates the average concentration of healthy people with active TGF-β of 1.23 ng/ml and PDGF-BB of 1.68 ng/ml. k Immunofluorescent staining and l quantitative analysis of CD73 + (green) and CD90 + cells (red) in bone marrow of ectopic bone marrow in HO patients. Blue color indicates DAPI staining of nuclei. Scale bar, 20 μm. C cartilage, B bone, BM bone marrow, M muscle, NM normal muscle, EF elbow fracture, CNST central nervous system trauma, OR osteogenesis MR maturation. All data are shown as the mean ± s.d. n = 9 per group for d , f , h , l histomorphometry analysis. * p
    Oil Red O Staining, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 489 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Johnson & Johnson o shea jj
    Elevated active TGF-β levels in human HO. a H E staining of normal muscle (left) and HO in early osteogenesis stage (middle) and late osteogenesis stage (right) after elbow fracture (EF) and central nervous system trauma (CNST). Scale bar, 100 μm. b <t>Safranin</t> O and Fast Green (SOFG) staining of normal muscle (left) and HO. Proteoglycan (red) and heterotopic bone (green). Scale bar, 100 μm. c TRAP + cells (red) and d quantitative analysis of the number of TRAP + osteoclast surface (OCS) per bone surface (BS). Scale bar, 100 μm. Immunohistochemical staining of e pSmad2/3 + cells (brown) and g PDGF-BB + cells (brown) and quantitative analysis of the number of f pSmad2/3 + cells or h PDGF-BB + cells per bone marrow area (mm 2 ). i , j Quantitative analysis of i active TGF-β and j PDGF-BB in serum in HO patients at early osteogenesis stage and late mature stage by ELISA. Dotted line indicates the average concentration of healthy people with active TGF-β of 1.23 ng/ml and PDGF-BB of 1.68 ng/ml. k Immunofluorescent staining and l quantitative analysis of CD73 + (green) and CD90 + cells (red) in bone marrow of ectopic bone marrow in HO patients. Blue color indicates DAPI staining of nuclei. Scale bar, 20 μm. C cartilage, B bone, BM bone marrow, M muscle, NM normal muscle, EF elbow fracture, CNST central nervous system trauma, OR osteogenesis MR maturation. All data are shown as the mean ± s.d. n = 9 per group for d , f , h , l histomorphometry analysis. * p
    O Shea Jj, supplied by Johnson & Johnson, used in various techniques. Bioz Stars score: 91/100, based on 243 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher dna ladder
    Elevated active TGF-β levels in human HO. a H E staining of normal muscle (left) and HO in early osteogenesis stage (middle) and late osteogenesis stage (right) after elbow fracture (EF) and central nervous system trauma (CNST). Scale bar, 100 μm. b <t>Safranin</t> O and Fast Green (SOFG) staining of normal muscle (left) and HO. Proteoglycan (red) and heterotopic bone (green). Scale bar, 100 μm. c TRAP + cells (red) and d quantitative analysis of the number of TRAP + osteoclast surface (OCS) per bone surface (BS). Scale bar, 100 μm. Immunohistochemical staining of e pSmad2/3 + cells (brown) and g PDGF-BB + cells (brown) and quantitative analysis of the number of f pSmad2/3 + cells or h PDGF-BB + cells per bone marrow area (mm 2 ). i , j Quantitative analysis of i active TGF-β and j PDGF-BB in serum in HO patients at early osteogenesis stage and late mature stage by ELISA. Dotted line indicates the average concentration of healthy people with active TGF-β of 1.23 ng/ml and PDGF-BB of 1.68 ng/ml. k Immunofluorescent staining and l quantitative analysis of CD73 + (green) and CD90 + cells (red) in bone marrow of ectopic bone marrow in HO patients. Blue color indicates DAPI staining of nuclei. Scale bar, 20 μm. C cartilage, B bone, BM bone marrow, M muscle, NM normal muscle, EF elbow fracture, CNST central nervous system trauma, OR osteogenesis MR maturation. All data are shown as the mean ± s.d. n = 9 per group for d , f , h , l histomorphometry analysis. * p
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    Millipore o nitrophenyl β d galactopyranoside
    Elevated active TGF-β levels in human HO. a H E staining of normal muscle (left) and HO in early osteogenesis stage (middle) and late osteogenesis stage (right) after elbow fracture (EF) and central nervous system trauma (CNST). Scale bar, 100 μm. b <t>Safranin</t> O and Fast Green (SOFG) staining of normal muscle (left) and HO. Proteoglycan (red) and heterotopic bone (green). Scale bar, 100 μm. c TRAP + cells (red) and d quantitative analysis of the number of TRAP + osteoclast surface (OCS) per bone surface (BS). Scale bar, 100 μm. Immunohistochemical staining of e pSmad2/3 + cells (brown) and g PDGF-BB + cells (brown) and quantitative analysis of the number of f pSmad2/3 + cells or h PDGF-BB + cells per bone marrow area (mm 2 ). i , j Quantitative analysis of i active TGF-β and j PDGF-BB in serum in HO patients at early osteogenesis stage and late mature stage by ELISA. Dotted line indicates the average concentration of healthy people with active TGF-β of 1.23 ng/ml and PDGF-BB of 1.68 ng/ml. k Immunofluorescent staining and l quantitative analysis of CD73 + (green) and CD90 + cells (red) in bone marrow of ectopic bone marrow in HO patients. Blue color indicates DAPI staining of nuclei. Scale bar, 20 μm. C cartilage, B bone, BM bone marrow, M muscle, NM normal muscle, EF elbow fracture, CNST central nervous system trauma, OR osteogenesis MR maturation. All data are shown as the mean ± s.d. n = 9 per group for d , f , h , l histomorphometry analysis. * p
    O Nitrophenyl β D Galactopyranoside, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 798 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore oil red o solution
    Stemness features of the side population. In image ( A ) respective panel ( D the left drawing), the colony-forming potential of SP compared to non-SP cells is illustrated. The image shows representative colonies. Panel ( B ) shows <t>OIL-RED-O-stained</t> cells after adipogenic differentiation. More dark red cells indicate more adipogeneic differentiation. The slightly smaller SP cells are seen in Panel ( C ) (May-Grünwald-Giemsa staining). In panel ( D ), the two rightmost diagrams show the higher resistance of SP cells compared to non-SP cells (reference no-sort) after treatment with cytotoxic drugs for 72 h. Doxorubicine was effective at 0.1 µM while Etoposide is shown at 1 µM. A star indicates a significant difference using the t test and an alpha error of 5%. ‘ n ’ denotes the number of biological replicates behind a condition. Horizontal brackets with a star indicate a significant difference between bars (alpha
    Oil Red O Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 470 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Elevated active TGF-β levels in human HO. a H E staining of normal muscle (left) and HO in early osteogenesis stage (middle) and late osteogenesis stage (right) after elbow fracture (EF) and central nervous system trauma (CNST). Scale bar, 100 μm. b Safranin O and Fast Green (SOFG) staining of normal muscle (left) and HO. Proteoglycan (red) and heterotopic bone (green). Scale bar, 100 μm. c TRAP + cells (red) and d quantitative analysis of the number of TRAP + osteoclast surface (OCS) per bone surface (BS). Scale bar, 100 μm. Immunohistochemical staining of e pSmad2/3 + cells (brown) and g PDGF-BB + cells (brown) and quantitative analysis of the number of f pSmad2/3 + cells or h PDGF-BB + cells per bone marrow area (mm 2 ). i , j Quantitative analysis of i active TGF-β and j PDGF-BB in serum in HO patients at early osteogenesis stage and late mature stage by ELISA. Dotted line indicates the average concentration of healthy people with active TGF-β of 1.23 ng/ml and PDGF-BB of 1.68 ng/ml. k Immunofluorescent staining and l quantitative analysis of CD73 + (green) and CD90 + cells (red) in bone marrow of ectopic bone marrow in HO patients. Blue color indicates DAPI staining of nuclei. Scale bar, 20 μm. C cartilage, B bone, BM bone marrow, M muscle, NM normal muscle, EF elbow fracture, CNST central nervous system trauma, OR osteogenesis MR maturation. All data are shown as the mean ± s.d. n = 9 per group for d , f , h , l histomorphometry analysis. * p

    Journal: Nature Communications

    Article Title: Inhibition of overactive TGF-β attenuates progression of heterotopic ossification in mice

    doi: 10.1038/s41467-018-02988-5

    Figure Lengend Snippet: Elevated active TGF-β levels in human HO. a H E staining of normal muscle (left) and HO in early osteogenesis stage (middle) and late osteogenesis stage (right) after elbow fracture (EF) and central nervous system trauma (CNST). Scale bar, 100 μm. b Safranin O and Fast Green (SOFG) staining of normal muscle (left) and HO. Proteoglycan (red) and heterotopic bone (green). Scale bar, 100 μm. c TRAP + cells (red) and d quantitative analysis of the number of TRAP + osteoclast surface (OCS) per bone surface (BS). Scale bar, 100 μm. Immunohistochemical staining of e pSmad2/3 + cells (brown) and g PDGF-BB + cells (brown) and quantitative analysis of the number of f pSmad2/3 + cells or h PDGF-BB + cells per bone marrow area (mm 2 ). i , j Quantitative analysis of i active TGF-β and j PDGF-BB in serum in HO patients at early osteogenesis stage and late mature stage by ELISA. Dotted line indicates the average concentration of healthy people with active TGF-β of 1.23 ng/ml and PDGF-BB of 1.68 ng/ml. k Immunofluorescent staining and l quantitative analysis of CD73 + (green) and CD90 + cells (red) in bone marrow of ectopic bone marrow in HO patients. Blue color indicates DAPI staining of nuclei. Scale bar, 20 μm. C cartilage, B bone, BM bone marrow, M muscle, NM normal muscle, EF elbow fracture, CNST central nervous system trauma, OR osteogenesis MR maturation. All data are shown as the mean ± s.d. n = 9 per group for d , f , h , l histomorphometry analysis. * p

    Article Snippet: We processed 4-μm-thick sections of bone for H & E staining and safranin o (Sigma-Aldrich, S2255) and fast green (Sigma-Aldrich, F7252) staining.

    Techniques: Staining, Immunohistochemistry, Enzyme-linked Immunosorbent Assay, Concentration Assay

    Stemness features of the side population. In image ( A ) respective panel ( D the left drawing), the colony-forming potential of SP compared to non-SP cells is illustrated. The image shows representative colonies. Panel ( B ) shows OIL-RED-O-stained cells after adipogenic differentiation. More dark red cells indicate more adipogeneic differentiation. The slightly smaller SP cells are seen in Panel ( C ) (May-Grünwald-Giemsa staining). In panel ( D ), the two rightmost diagrams show the higher resistance of SP cells compared to non-SP cells (reference no-sort) after treatment with cytotoxic drugs for 72 h. Doxorubicine was effective at 0.1 µM while Etoposide is shown at 1 µM. A star indicates a significant difference using the t test and an alpha error of 5%. ‘ n ’ denotes the number of biological replicates behind a condition. Horizontal brackets with a star indicate a significant difference between bars (alpha

    Journal: International Journal of Molecular Sciences

    Article Title: Defining a Characteristic Gene Expression Set Responsible for Cancer Stem Cell-Like Features in a Sub-Population of Ewing Sarcoma Cells CADO-ES1

    doi: 10.3390/ijms19123908

    Figure Lengend Snippet: Stemness features of the side population. In image ( A ) respective panel ( D the left drawing), the colony-forming potential of SP compared to non-SP cells is illustrated. The image shows representative colonies. Panel ( B ) shows OIL-RED-O-stained cells after adipogenic differentiation. More dark red cells indicate more adipogeneic differentiation. The slightly smaller SP cells are seen in Panel ( C ) (May-Grünwald-Giemsa staining). In panel ( D ), the two rightmost diagrams show the higher resistance of SP cells compared to non-SP cells (reference no-sort) after treatment with cytotoxic drugs for 72 h. Doxorubicine was effective at 0.1 µM while Etoposide is shown at 1 µM. A star indicates a significant difference using the t test and an alpha error of 5%. ‘ n ’ denotes the number of biological replicates behind a condition. Horizontal brackets with a star indicate a significant difference between bars (alpha

    Article Snippet: These two medium changes were repeated a second time and finally cells were stained with OIL-RED-O solution (Sigma-Aldrich).

    Techniques: Staining