nuli Search Results


human bronchial epithelium  (ATCC)
93
ATCC human bronchial epithelium
Human Bronchial Epithelium, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human bronchial epithelium/product/ATCC
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
human bronchial epithelium - by Bioz Stars, 2024-10
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nuli cells  (Corning Life Sciences)
86
Corning Life Sciences nuli cells
Coimmunoprecipitation of endogenous AMPK-γ1 and KCa3.1 in <t>NuLi</t> <t>cells.</t> Immunoblots showing AMPK-γ1 (A, B) and KCa3.1 (C, D) proteins from NuLi extracts and AMPK-γ1/KCa3.1 immunoprecipitations. Membranes were blotted with anti-AMPK-γ1 (A, B) and anti-KCa3.1 (C, D) antibodies, in the presence (B, D) or absence (A, C) of neutralizing peptide. Endogenous expression of AMPK-γ1 and KCa3.1 proteins in the NuLi cell lysate are presented in lanes 4 of A and C, respectively. Control lanes (A and C, lanes 1) refer to coimmunoprecipitation experiments done in the absence of immunoprecipitating antibody. Control immunoprecipitations were also performed, AMPK-γ1 immunoprecipitation with the AMPK-γ1 antibody (A, lane 2) and KCa3.1 immmunoprecipitation with the KCa3.1 antibody (C, lane 3). Coimmunoprecipitation of endogenous AMPK-γ1 using anti-KCa3.1 antibody is illustrated in A, lane 3. Conversely, coimmunoprecipitation of endogenous KCa3.1 using anti-AMPK-γ1 antibody is shown at C, lane 2. Note that the same lysate and IP samples were used in the upper and lower parts of the membranes, blotted with AMPK-γ1 and KCa3.1 antibodies, respectively. Because of the higher AMPK-γ1 signal in cell lysates, 1/3 of the total IP was loaded for AMPK-γ1 immunoblot, whereas 2/3 of the total IP was loaded for the KCa3.1 immunoblot. No band was observed when the membranes were blotted with anti-AMPK-γ1 or anti-KCa3.1 antibodies in the presence of their respective neutralizing peptides (B and D).
Nuli Cells, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nuli cells/product/Corning Life Sciences
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
nuli cells - by Bioz Stars, 2024-10
86/100 stars
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nuli 1 cells  (Thermo Fisher)
86
Thermo Fisher nuli 1 cells
Coimmunoprecipitation of endogenous AMPK-γ1 and KCa3.1 in <t>NuLi</t> <t>cells.</t> Immunoblots showing AMPK-γ1 (A, B) and KCa3.1 (C, D) proteins from NuLi extracts and AMPK-γ1/KCa3.1 immunoprecipitations. Membranes were blotted with anti-AMPK-γ1 (A, B) and anti-KCa3.1 (C, D) antibodies, in the presence (B, D) or absence (A, C) of neutralizing peptide. Endogenous expression of AMPK-γ1 and KCa3.1 proteins in the NuLi cell lysate are presented in lanes 4 of A and C, respectively. Control lanes (A and C, lanes 1) refer to coimmunoprecipitation experiments done in the absence of immunoprecipitating antibody. Control immunoprecipitations were also performed, AMPK-γ1 immunoprecipitation with the AMPK-γ1 antibody (A, lane 2) and KCa3.1 immmunoprecipitation with the KCa3.1 antibody (C, lane 3). Coimmunoprecipitation of endogenous AMPK-γ1 using anti-KCa3.1 antibody is illustrated in A, lane 3. Conversely, coimmunoprecipitation of endogenous KCa3.1 using anti-AMPK-γ1 antibody is shown at C, lane 2. Note that the same lysate and IP samples were used in the upper and lower parts of the membranes, blotted with AMPK-γ1 and KCa3.1 antibodies, respectively. Because of the higher AMPK-γ1 signal in cell lysates, 1/3 of the total IP was loaded for AMPK-γ1 immunoblot, whereas 2/3 of the total IP was loaded for the KCa3.1 immunoblot. No band was observed when the membranes were blotted with anti-AMPK-γ1 or anti-KCa3.1 antibodies in the presence of their respective neutralizing peptides (B and D).
Nuli 1 Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nuli 1 cells/product/Thermo Fisher
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
nuli 1 cells - by Bioz Stars, 2024-10
86/100 stars
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nuli cufi cells  (Tecan Systems)
86
Tecan Systems nuli cufi cells
Coimmunoprecipitation of endogenous AMPK-γ1 and KCa3.1 in <t>NuLi</t> <t>cells.</t> Immunoblots showing AMPK-γ1 (A, B) and KCa3.1 (C, D) proteins from NuLi extracts and AMPK-γ1/KCa3.1 immunoprecipitations. Membranes were blotted with anti-AMPK-γ1 (A, B) and anti-KCa3.1 (C, D) antibodies, in the presence (B, D) or absence (A, C) of neutralizing peptide. Endogenous expression of AMPK-γ1 and KCa3.1 proteins in the NuLi cell lysate are presented in lanes 4 of A and C, respectively. Control lanes (A and C, lanes 1) refer to coimmunoprecipitation experiments done in the absence of immunoprecipitating antibody. Control immunoprecipitations were also performed, AMPK-γ1 immunoprecipitation with the AMPK-γ1 antibody (A, lane 2) and KCa3.1 immmunoprecipitation with the KCa3.1 antibody (C, lane 3). Coimmunoprecipitation of endogenous AMPK-γ1 using anti-KCa3.1 antibody is illustrated in A, lane 3. Conversely, coimmunoprecipitation of endogenous KCa3.1 using anti-AMPK-γ1 antibody is shown at C, lane 2. Note that the same lysate and IP samples were used in the upper and lower parts of the membranes, blotted with AMPK-γ1 and KCa3.1 antibodies, respectively. Because of the higher AMPK-γ1 signal in cell lysates, 1/3 of the total IP was loaded for AMPK-γ1 immunoblot, whereas 2/3 of the total IP was loaded for the KCa3.1 immunoblot. No band was observed when the membranes were blotted with anti-AMPK-γ1 or anti-KCa3.1 antibodies in the presence of their respective neutralizing peptides (B and D).
Nuli Cufi Cells, supplied by Tecan Systems, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nuli cufi cells/product/Tecan Systems
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
nuli cufi cells - by Bioz Stars, 2024-10
86/100 stars
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Image Search Results


Coimmunoprecipitation of endogenous AMPK-γ1 and KCa3.1 in NuLi cells. Immunoblots showing AMPK-γ1 (A, B) and KCa3.1 (C, D) proteins from NuLi extracts and AMPK-γ1/KCa3.1 immunoprecipitations. Membranes were blotted with anti-AMPK-γ1 (A, B) and anti-KCa3.1 (C, D) antibodies, in the presence (B, D) or absence (A, C) of neutralizing peptide. Endogenous expression of AMPK-γ1 and KCa3.1 proteins in the NuLi cell lysate are presented in lanes 4 of A and C, respectively. Control lanes (A and C, lanes 1) refer to coimmunoprecipitation experiments done in the absence of immunoprecipitating antibody. Control immunoprecipitations were also performed, AMPK-γ1 immunoprecipitation with the AMPK-γ1 antibody (A, lane 2) and KCa3.1 immmunoprecipitation with the KCa3.1 antibody (C, lane 3). Coimmunoprecipitation of endogenous AMPK-γ1 using anti-KCa3.1 antibody is illustrated in A, lane 3. Conversely, coimmunoprecipitation of endogenous KCa3.1 using anti-AMPK-γ1 antibody is shown at C, lane 2. Note that the same lysate and IP samples were used in the upper and lower parts of the membranes, blotted with AMPK-γ1 and KCa3.1 antibodies, respectively. Because of the higher AMPK-γ1 signal in cell lysates, 1/3 of the total IP was loaded for AMPK-γ1 immunoblot, whereas 2/3 of the total IP was loaded for the KCa3.1 immunoblot. No band was observed when the membranes were blotted with anti-AMPK-γ1 or anti-KCa3.1 antibodies in the presence of their respective neutralizing peptides (B and D).

Journal:

Article Title: Inhibition of the KCa3.1 channels by AMP-activated protein kinase in human airway epithelial cells

doi: 10.1152/ajpcell.00418.2008

Figure Lengend Snippet: Coimmunoprecipitation of endogenous AMPK-γ1 and KCa3.1 in NuLi cells. Immunoblots showing AMPK-γ1 (A, B) and KCa3.1 (C, D) proteins from NuLi extracts and AMPK-γ1/KCa3.1 immunoprecipitations. Membranes were blotted with anti-AMPK-γ1 (A, B) and anti-KCa3.1 (C, D) antibodies, in the presence (B, D) or absence (A, C) of neutralizing peptide. Endogenous expression of AMPK-γ1 and KCa3.1 proteins in the NuLi cell lysate are presented in lanes 4 of A and C, respectively. Control lanes (A and C, lanes 1) refer to coimmunoprecipitation experiments done in the absence of immunoprecipitating antibody. Control immunoprecipitations were also performed, AMPK-γ1 immunoprecipitation with the AMPK-γ1 antibody (A, lane 2) and KCa3.1 immmunoprecipitation with the KCa3.1 antibody (C, lane 3). Coimmunoprecipitation of endogenous AMPK-γ1 using anti-KCa3.1 antibody is illustrated in A, lane 3. Conversely, coimmunoprecipitation of endogenous KCa3.1 using anti-AMPK-γ1 antibody is shown at C, lane 2. Note that the same lysate and IP samples were used in the upper and lower parts of the membranes, blotted with AMPK-γ1 and KCa3.1 antibodies, respectively. Because of the higher AMPK-γ1 signal in cell lysates, 1/3 of the total IP was loaded for AMPK-γ1 immunoblot, whereas 2/3 of the total IP was loaded for the KCa3.1 immunoblot. No band was observed when the membranes were blotted with anti-AMPK-γ1 or anti-KCa3.1 antibodies in the presence of their respective neutralizing peptides (B and D).

Article Snippet: For electrophysiological experiments, NuLi cells were grown on Costar Transwell permeant filters (Costar Transwell, Toronto, ON) at the air-liquid interface in DMEM-F12 medium (Invitrogen) supplemented with UltroSerG (Biosepra SPA, Cergy-Saint-Christophe, France), which enhances ion transport ( 50 ).

Techniques: Western Blot, Expressing, Immunoprecipitation