nuclease-free water Qiagen Search Results


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  • 95
    Qiagen nuclease free water nfw
    Comparison of ‘wet’ and lyophilized reagents using direct detection by RT ‐ LAMP with primer set 1 (a) and primer set 2 (b). Black bars represent ‘wet’ reagents and grey bars represent lyophilized reagents. Neat: SVV ‐1 sample NC ‐88‐23626 diluted 1/100 in negative pig epithelium tissue suspension to simulate a natural original suspension sample. This ‘neat’ sample was then diluted frac12;, ¼, 1/8, 1/10, 1/16 and 1/20 in nuclease‐free water ( <t>NFW</t> ) and compared to extracted <t>RNA</t> from the ‘neat’ sample as a positive control
    Nuclease Free Water Nfw, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher nuclease free water
    Comparison of ‘wet’ and lyophilized reagents using direct detection by RT ‐ LAMP with primer set 1 (a) and primer set 2 (b). Black bars represent ‘wet’ reagents and grey bars represent lyophilized reagents. Neat: SVV ‐1 sample NC ‐88‐23626 diluted 1/100 in negative pig epithelium tissue suspension to simulate a natural original suspension sample. This ‘neat’ sample was then diluted frac12;, ¼, 1/8, 1/10, 1/16 and 1/20 in nuclease‐free water ( <t>NFW</t> ) and compared to extracted <t>RNA</t> from the ‘neat’ sample as a positive control
    Nuclease Free Water, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 14597 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore nuclease free water
    Comparison of ‘wet’ and lyophilized reagents using direct detection by RT ‐ LAMP with primer set 1 (a) and primer set 2 (b). Black bars represent ‘wet’ reagents and grey bars represent lyophilized reagents. Neat: SVV ‐1 sample NC ‐88‐23626 diluted 1/100 in negative pig epithelium tissue suspension to simulate a natural original suspension sample. This ‘neat’ sample was then diluted frac12;, ¼, 1/8, 1/10, 1/16 and 1/20 in nuclease‐free water ( <t>NFW</t> ) and compared to extracted <t>RNA</t> from the ‘neat’ sample as a positive control
    Nuclease Free Water, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1370 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Qiagen rnase free water
    Evaluation of the miRNeasy (Qiagen) and miRCURY (Exiqon) extraction kits. ( A ) Eight healthy blood donors were used. <t>RNA</t> was extracted from 200 µL serum using the miRNeasy RNA isolation kit (Qiagen) and miRCURY RNA isolation kit Biofuids (Exiqon) following the manufacturer’s instructions, and eluted in 14 µL and 50 µL of <t>RNase</t> free water respectively. TaqMan microRNA quantitative reverse transcription polymerase chain reaction (qRT-PCR) (Applied Biosystems, Waltham, MA, USA) was carried out against miR-423-3p, a normalizer recommended by Exiqon. Cq values for miR-423-3p obtained from RNA extracted using the miRNeasy RNA isolation kit (Red) verses Cq values obtained from RNA extracted using the miRCURY RNA isolation kit Biofluids (Green) are displayed. ( B ). *** p
    Rnase Free Water, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 3381 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Qiagen pcr water
    Evaluation of the miRNeasy (Qiagen) and miRCURY (Exiqon) extraction kits. ( A ) Eight healthy blood donors were used. <t>RNA</t> was extracted from 200 µL serum using the miRNeasy RNA isolation kit (Qiagen) and miRCURY RNA isolation kit Biofuids (Exiqon) following the manufacturer’s instructions, and eluted in 14 µL and 50 µL of <t>RNase</t> free water respectively. TaqMan microRNA quantitative reverse transcription polymerase chain reaction (qRT-PCR) (Applied Biosystems, Waltham, MA, USA) was carried out against miR-423-3p, a normalizer recommended by Exiqon. Cq values for miR-423-3p obtained from RNA extracted using the miRNeasy RNA isolation kit (Red) verses Cq values obtained from RNA extracted using the miRCURY RNA isolation kit Biofluids (Green) are displayed. ( B ). *** p
    Pcr Water, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 97 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Qiagen rnase water
    Evaluation of the miRNeasy (Qiagen) and miRCURY (Exiqon) extraction kits. ( A ) Eight healthy blood donors were used. <t>RNA</t> was extracted from 200 µL serum using the miRNeasy RNA isolation kit (Qiagen) and miRCURY RNA isolation kit Biofuids (Exiqon) following the manufacturer’s instructions, and eluted in 14 µL and 50 µL of <t>RNase</t> free water respectively. TaqMan microRNA quantitative reverse transcription polymerase chain reaction (qRT-PCR) (Applied Biosystems, Waltham, MA, USA) was carried out against miR-423-3p, a normalizer recommended by Exiqon. Cq values for miR-423-3p obtained from RNA extracted using the miRNeasy RNA isolation kit (Red) verses Cq values obtained from RNA extracted using the miRCURY RNA isolation kit Biofluids (Green) are displayed. ( B ). *** p
    Rnase Water, supplied by Qiagen, used in various techniques. Bioz Stars score: 97/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Qiagen dnase free water
    Evaluation of the miRNeasy (Qiagen) and miRCURY (Exiqon) extraction kits. ( A ) Eight healthy blood donors were used. <t>RNA</t> was extracted from 200 µL serum using the miRNeasy RNA isolation kit (Qiagen) and miRCURY RNA isolation kit Biofuids (Exiqon) following the manufacturer’s instructions, and eluted in 14 µL and 50 µL of <t>RNase</t> free water respectively. TaqMan microRNA quantitative reverse transcription polymerase chain reaction (qRT-PCR) (Applied Biosystems, Waltham, MA, USA) was carried out against miR-423-3p, a normalizer recommended by Exiqon. Cq values for miR-423-3p obtained from RNA extracted using the miRNeasy RNA isolation kit (Red) verses Cq values obtained from RNA extracted using the miRCURY RNA isolation kit Biofluids (Green) are displayed. ( B ). *** p
    Dnase Free Water, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 103 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Avantor dnase free ultra pure water
    Evaluation of the miRNeasy (Qiagen) and miRCURY (Exiqon) extraction kits. ( A ) Eight healthy blood donors were used. <t>RNA</t> was extracted from 200 µL serum using the miRNeasy RNA isolation kit (Qiagen) and miRCURY RNA isolation kit Biofuids (Exiqon) following the manufacturer’s instructions, and eluted in 14 µL and 50 µL of <t>RNase</t> free water respectively. TaqMan microRNA quantitative reverse transcription polymerase chain reaction (qRT-PCR) (Applied Biosystems, Waltham, MA, USA) was carried out against miR-423-3p, a normalizer recommended by Exiqon. Cq values for miR-423-3p obtained from RNA extracted using the miRNeasy RNA isolation kit (Red) verses Cq values obtained from RNA extracted using the miRCURY RNA isolation kit Biofluids (Green) are displayed. ( B ). *** p
    Dnase Free Ultra Pure Water, supplied by Avantor, used in various techniques. Bioz Stars score: 86/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Qiagen dnaase rnaase free water
    Evaluation of the miRNeasy (Qiagen) and miRCURY (Exiqon) extraction kits. ( A ) Eight healthy blood donors were used. <t>RNA</t> was extracted from 200 µL serum using the miRNeasy RNA isolation kit (Qiagen) and miRCURY RNA isolation kit Biofuids (Exiqon) following the manufacturer’s instructions, and eluted in 14 µL and 50 µL of <t>RNase</t> free water respectively. TaqMan microRNA quantitative reverse transcription polymerase chain reaction (qRT-PCR) (Applied Biosystems, Waltham, MA, USA) was carried out against miR-423-3p, a normalizer recommended by Exiqon. Cq values for miR-423-3p obtained from RNA extracted using the miRNeasy RNA isolation kit (Red) verses Cq values obtained from RNA extracted using the miRCURY RNA isolation kit Biofluids (Green) are displayed. ( B ). *** p
    Dnaase Rnaase Free Water, supplied by Qiagen, used in various techniques. Bioz Stars score: 80/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Qiagen sterile dnase free water
    Evaluation of the miRNeasy (Qiagen) and miRCURY (Exiqon) extraction kits. ( A ) Eight healthy blood donors were used. <t>RNA</t> was extracted from 200 µL serum using the miRNeasy RNA isolation kit (Qiagen) and miRCURY RNA isolation kit Biofuids (Exiqon) following the manufacturer’s instructions, and eluted in 14 µL and 50 µL of <t>RNase</t> free water respectively. TaqMan microRNA quantitative reverse transcription polymerase chain reaction (qRT-PCR) (Applied Biosystems, Waltham, MA, USA) was carried out against miR-423-3p, a normalizer recommended by Exiqon. Cq values for miR-423-3p obtained from RNA extracted using the miRNeasy RNA isolation kit (Red) verses Cq values obtained from RNA extracted using the miRCURY RNA isolation kit Biofluids (Green) are displayed. ( B ). *** p
    Sterile Dnase Free Water, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    76
    Thermo Fisher absolute ethanol rnase free water instrument
    Evaluation of the miRNeasy (Qiagen) and miRCURY (Exiqon) extraction kits. ( A ) Eight healthy blood donors were used. <t>RNA</t> was extracted from 200 µL serum using the miRNeasy RNA isolation kit (Qiagen) and miRCURY RNA isolation kit Biofuids (Exiqon) following the manufacturer’s instructions, and eluted in 14 µL and 50 µL of <t>RNase</t> free water respectively. TaqMan microRNA quantitative reverse transcription polymerase chain reaction (qRT-PCR) (Applied Biosystems, Waltham, MA, USA) was carried out against miR-423-3p, a normalizer recommended by Exiqon. Cq values for miR-423-3p obtained from RNA extracted using the miRNeasy RNA isolation kit (Red) verses Cq values obtained from RNA extracted using the miRCURY RNA isolation kit Biofluids (Green) are displayed. ( B ). *** p
    Absolute Ethanol Rnase Free Water Instrument, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 76/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Qiagen dnase rnase free distilled water
    Evaluation of the miRNeasy (Qiagen) and miRCURY (Exiqon) extraction kits. ( A ) Eight healthy blood donors were used. <t>RNA</t> was extracted from 200 µL serum using the miRNeasy RNA isolation kit (Qiagen) and miRCURY RNA isolation kit Biofuids (Exiqon) following the manufacturer’s instructions, and eluted in 14 µL and 50 µL of <t>RNase</t> free water respectively. TaqMan microRNA quantitative reverse transcription polymerase chain reaction (qRT-PCR) (Applied Biosystems, Waltham, MA, USA) was carried out against miR-423-3p, a normalizer recommended by Exiqon. Cq values for miR-423-3p obtained from RNA extracted using the miRNeasy RNA isolation kit (Red) verses Cq values obtained from RNA extracted using the miRCURY RNA isolation kit Biofluids (Green) are displayed. ( B ). *** p
    Dnase Rnase Free Distilled Water, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 66 article reviews
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    87
    Qiagen dnase rnase free molecular grade water
    Evaluation of the miRNeasy (Qiagen) and miRCURY (Exiqon) extraction kits. ( A ) Eight healthy blood donors were used. <t>RNA</t> was extracted from 200 µL serum using the miRNeasy RNA isolation kit (Qiagen) and miRCURY RNA isolation kit Biofuids (Exiqon) following the manufacturer’s instructions, and eluted in 14 µL and 50 µL of <t>RNase</t> free water respectively. TaqMan microRNA quantitative reverse transcription polymerase chain reaction (qRT-PCR) (Applied Biosystems, Waltham, MA, USA) was carried out against miR-423-3p, a normalizer recommended by Exiqon. Cq values for miR-423-3p obtained from RNA extracted using the miRNeasy RNA isolation kit (Red) verses Cq values obtained from RNA extracted using the miRCURY RNA isolation kit Biofluids (Green) are displayed. ( B ). *** p
    Dnase Rnase Free Molecular Grade Water, supplied by Qiagen, used in various techniques. Bioz Stars score: 87/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Qiagen rnase free dnase set
    Evaluation of the miRNeasy (Qiagen) and miRCURY (Exiqon) extraction kits. ( A ) Eight healthy blood donors were used. <t>RNA</t> was extracted from 200 µL serum using the miRNeasy RNA isolation kit (Qiagen) and miRCURY RNA isolation kit Biofuids (Exiqon) following the manufacturer’s instructions, and eluted in 14 µL and 50 µL of <t>RNase</t> free water respectively. TaqMan microRNA quantitative reverse transcription polymerase chain reaction (qRT-PCR) (Applied Biosystems, Waltham, MA, USA) was carried out against miR-423-3p, a normalizer recommended by Exiqon. Cq values for miR-423-3p obtained from RNA extracted using the miRNeasy RNA isolation kit (Red) verses Cq values obtained from RNA extracted using the miRCURY RNA isolation kit Biofluids (Green) are displayed. ( B ). *** p
    Rnase Free Dnase Set, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 18433 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Qiagen dneasy
    Evaluation of the miRNeasy (Qiagen) and miRCURY (Exiqon) extraction kits. ( A ) Eight healthy blood donors were used. <t>RNA</t> was extracted from 200 µL serum using the miRNeasy RNA isolation kit (Qiagen) and miRCURY RNA isolation kit Biofuids (Exiqon) following the manufacturer’s instructions, and eluted in 14 µL and 50 µL of <t>RNase</t> free water respectively. TaqMan microRNA quantitative reverse transcription polymerase chain reaction (qRT-PCR) (Applied Biosystems, Waltham, MA, USA) was carried out against miR-423-3p, a normalizer recommended by Exiqon. Cq values for miR-423-3p obtained from RNA extracted using the miRNeasy RNA isolation kit (Red) verses Cq values obtained from RNA extracted using the miRCURY RNA isolation kit Biofluids (Green) are displayed. ( B ). *** p
    Dneasy, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1520 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dneasy/product/Qiagen
    Average 99 stars, based on 1520 article reviews
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    dneasy - by Bioz Stars, 2020-02
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    95
    Qiagen rnase
    Evaluation of the miRNeasy (Qiagen) and miRCURY (Exiqon) extraction kits. ( A ) Eight healthy blood donors were used. <t>RNA</t> was extracted from 200 µL serum using the miRNeasy RNA isolation kit (Qiagen) and miRCURY RNA isolation kit Biofuids (Exiqon) following the manufacturer’s instructions, and eluted in 14 µL and 50 µL of <t>RNase</t> free water respectively. TaqMan microRNA quantitative reverse transcription polymerase chain reaction (qRT-PCR) (Applied Biosystems, Waltham, MA, USA) was carried out against miR-423-3p, a normalizer recommended by Exiqon. Cq values for miR-423-3p obtained from RNA extracted using the miRNeasy RNA isolation kit (Red) verses Cq values obtained from RNA extracted using the miRCURY RNA isolation kit Biofluids (Green) are displayed. ( B ). *** p
    Rnase, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 2607 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Qiagen hotstartaq master mix
    Evaluation of the miRNeasy (Qiagen) and miRCURY (Exiqon) extraction kits. ( A ) Eight healthy blood donors were used. <t>RNA</t> was extracted from 200 µL serum using the miRNeasy RNA isolation kit (Qiagen) and miRCURY RNA isolation kit Biofuids (Exiqon) following the manufacturer’s instructions, and eluted in 14 µL and 50 µL of <t>RNase</t> free water respectively. TaqMan microRNA quantitative reverse transcription polymerase chain reaction (qRT-PCR) (Applied Biosystems, Waltham, MA, USA) was carried out against miR-423-3p, a normalizer recommended by Exiqon. Cq values for miR-423-3p obtained from RNA extracted using the miRNeasy RNA isolation kit (Red) verses Cq values obtained from RNA extracted using the miRCURY RNA isolation kit Biofluids (Green) are displayed. ( B ). *** p
    Hotstartaq Master Mix, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 2390 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Qiagen dnase stock solution
    Evaluation of the miRNeasy (Qiagen) and miRCURY (Exiqon) extraction kits. ( A ) Eight healthy blood donors were used. <t>RNA</t> was extracted from 200 µL serum using the miRNeasy RNA isolation kit (Qiagen) and miRCURY RNA isolation kit Biofuids (Exiqon) following the manufacturer’s instructions, and eluted in 14 µL and 50 µL of <t>RNase</t> free water respectively. TaqMan microRNA quantitative reverse transcription polymerase chain reaction (qRT-PCR) (Applied Biosystems, Waltham, MA, USA) was carried out against miR-423-3p, a normalizer recommended by Exiqon. Cq values for miR-423-3p obtained from RNA extracted using the miRNeasy RNA isolation kit (Red) verses Cq values obtained from RNA extracted using the miRCURY RNA isolation kit Biofluids (Green) are displayed. ( B ). *** p
    Dnase Stock Solution, supplied by Qiagen, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Qiagen rotor gene sybr green pcr kit
    Evaluation of the miRNeasy (Qiagen) and miRCURY (Exiqon) extraction kits. ( A ) Eight healthy blood donors were used. <t>RNA</t> was extracted from 200 µL serum using the miRNeasy RNA isolation kit (Qiagen) and miRCURY RNA isolation kit Biofuids (Exiqon) following the manufacturer’s instructions, and eluted in 14 µL and 50 µL of <t>RNase</t> free water respectively. TaqMan microRNA quantitative reverse transcription polymerase chain reaction (qRT-PCR) (Applied Biosystems, Waltham, MA, USA) was carried out against miR-423-3p, a normalizer recommended by Exiqon. Cq values for miR-423-3p obtained from RNA extracted using the miRNeasy RNA isolation kit (Red) verses Cq values obtained from RNA extracted using the miRCURY RNA isolation kit Biofluids (Green) are displayed. ( B ). *** p
    Rotor Gene Sybr Green Pcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 1933 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Qiagen rneasy mini kit
    Evaluation of the miRNeasy (Qiagen) and miRCURY (Exiqon) extraction kits. ( A ) Eight healthy blood donors were used. <t>RNA</t> was extracted from 200 µL serum using the miRNeasy RNA isolation kit (Qiagen) and miRCURY RNA isolation kit Biofuids (Exiqon) following the manufacturer’s instructions, and eluted in 14 µL and 50 µL of <t>RNase</t> free water respectively. TaqMan microRNA quantitative reverse transcription polymerase chain reaction (qRT-PCR) (Applied Biosystems, Waltham, MA, USA) was carried out against miR-423-3p, a normalizer recommended by Exiqon. Cq values for miR-423-3p obtained from RNA extracted using the miRNeasy RNA isolation kit (Red) verses Cq values obtained from RNA extracted using the miRCURY RNA isolation kit Biofluids (Green) are displayed. ( B ). *** p
    Rneasy Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 266299 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Comparison of ‘wet’ and lyophilized reagents using direct detection by RT ‐ LAMP with primer set 1 (a) and primer set 2 (b). Black bars represent ‘wet’ reagents and grey bars represent lyophilized reagents. Neat: SVV ‐1 sample NC ‐88‐23626 diluted 1/100 in negative pig epithelium tissue suspension to simulate a natural original suspension sample. This ‘neat’ sample was then diluted frac12;, ¼, 1/8, 1/10, 1/16 and 1/20 in nuclease‐free water ( NFW ) and compared to extracted RNA from the ‘neat’ sample as a positive control

    Journal: Transboundary and Emerging Diseases

    Article Title: The development of two field‐ready reverse transcription loop‐mediated isothermal amplification assays for the rapid detection of Seneca Valley virus 1. The development of two field‐ready reverse transcription loop‐mediated isothermal amplification assays for the rapid detection of Seneca Valley virus 1

    doi: 10.1111/tbed.13051

    Figure Lengend Snippet: Comparison of ‘wet’ and lyophilized reagents using direct detection by RT ‐ LAMP with primer set 1 (a) and primer set 2 (b). Black bars represent ‘wet’ reagents and grey bars represent lyophilized reagents. Neat: SVV ‐1 sample NC ‐88‐23626 diluted 1/100 in negative pig epithelium tissue suspension to simulate a natural original suspension sample. This ‘neat’ sample was then diluted frac12;, ¼, 1/8, 1/10, 1/16 and 1/20 in nuclease‐free water ( NFW ) and compared to extracted RNA from the ‘neat’ sample as a positive control

    Article Snippet: To determine the analytical sensitivity, a tenfold dilution series (10−1 –10−9 ) was made of RNA extracted from SVV‐1 isolates NC‐88‐23626 and LA‐97‐1278 (Table ) diluted in nuclease‐free water (NFW) containing carrier RNA (1 μg/μl, Qiagen).

    Techniques: Positive Control

    Evaluation of the miRNeasy (Qiagen) and miRCURY (Exiqon) extraction kits. ( A ) Eight healthy blood donors were used. RNA was extracted from 200 µL serum using the miRNeasy RNA isolation kit (Qiagen) and miRCURY RNA isolation kit Biofuids (Exiqon) following the manufacturer’s instructions, and eluted in 14 µL and 50 µL of RNase free water respectively. TaqMan microRNA quantitative reverse transcription polymerase chain reaction (qRT-PCR) (Applied Biosystems, Waltham, MA, USA) was carried out against miR-423-3p, a normalizer recommended by Exiqon. Cq values for miR-423-3p obtained from RNA extracted using the miRNeasy RNA isolation kit (Red) verses Cq values obtained from RNA extracted using the miRCURY RNA isolation kit Biofluids (Green) are displayed. ( B ). *** p

    Journal: Biomolecules

    Article Title: Circulating MicroRNA Biomarkers in Melanoma: Tools and Challenges in Personalised Medicine

    doi: 10.3390/biom8020021

    Figure Lengend Snippet: Evaluation of the miRNeasy (Qiagen) and miRCURY (Exiqon) extraction kits. ( A ) Eight healthy blood donors were used. RNA was extracted from 200 µL serum using the miRNeasy RNA isolation kit (Qiagen) and miRCURY RNA isolation kit Biofuids (Exiqon) following the manufacturer’s instructions, and eluted in 14 µL and 50 µL of RNase free water respectively. TaqMan microRNA quantitative reverse transcription polymerase chain reaction (qRT-PCR) (Applied Biosystems, Waltham, MA, USA) was carried out against miR-423-3p, a normalizer recommended by Exiqon. Cq values for miR-423-3p obtained from RNA extracted using the miRNeasy RNA isolation kit (Red) verses Cq values obtained from RNA extracted using the miRCURY RNA isolation kit Biofluids (Green) are displayed. ( B ). *** p

    Article Snippet: The difference in Cq observed between kits in a is expected as RNA is eluted in 3.57× the amount of RNase free water using the miRCURY RNA isolation kit (Exiqon) compared to the miRNeasy RNA isolation kit (Qiagen), resulting in a lower concentration.

    Techniques: Isolation, Reverse Transcription Polymerase Chain Reaction, Quantitative RT-PCR