Journal: Scientific Reports
Article Title: Structural and Functional Analysis of DDX41: a bispecific immune receptor for DNA and cyclic dinucleotide
Figure Lengend Snippet: The DDX41 DEAD domain binds to dsDNA and CDN. ( A ) FL-DDX41 and its truncated variants. DDX41 contains the DEAD domain (sky blue), the HELICc domain (orange) and the Zinc finger (ZF, gray). ( B ) Pull-down assays of DDX41, using bio-ISD (left panel) and bio-cGG (right panel). In all of the pull-down assays, the final concentration of bio-ISD, bio-cGG, bio-ATP or bio-AMPPNP is 1 μM, unless otherwise stated. Gels were run under the same experimental conditions and are shown as cropped gels. Full-length gels with indicated cropping lines are shown in Figure S7 .
Article Snippet: Bio-ISD, bio-cGG (2′-O- (6-[biotinyl]aminohexylcarbamoyl)-cyclic diguanosine monophosphate, B098-001, BioLog), bio-ATP (N6 -(6-amino)hexyl-adenosine-5′-triphosphate-biotin, NU-805-BIO, Jena Bioscience) and bio-AMPPNP (2′/3′-O-(2-aminoethyl-carbamoyl)-adenosine-5′-[(β,γ)-imido] triphosphate-biotin, NU-810-BIO, Jena Bioscience), each at a final concentration of 1 μM, were incubated with Dynabeads M-280 Streptavidin (Thermo Fisher Scientific) for 30 min at 4 °C in binding buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10% glycerol, 0.5 mM EDTA, 1 mM 2-mercaptoethanol, 0.5% Nonidet P-40).
Techniques: Concentration Assay