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  • nrcam  (Abcam)
    92
    Abcam nrcam
    <t>NrCAM</t> associates with Npn-2 through the TARNER sequence in Ig1. A , Postulated mechanism of <t>NrCAM/Npn-2/PlexA3</t> as a receptor complex for Sema3F in inducing dendritic spine retraction in cortical pyramidal neurons. The sequence TARNER within the Ig1 domain
    Nrcam, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Millipore rabbit anti ncam
    Sorted PAX2 -null human nephron progenitors undergo mesenchymal-to-epithelial transition. ( A ) LHX1 + renal vesicles are induced from both the wild-type and PAX2-null nephron progenitors at day 3 of co-culture with spinal cord. ( B ) E-CADHERIN (ECAD) + distal and CADHERIN6 (CDH6) + proximal renal tubules, as well as <t>NCAM</t> + S-shaped bodies, are formed from the wild-type and PAX2 -null nephron progenitors at day 6. ( C ) Renal tubules and glomeruli are formed at day 9. Left panels: ECAD + distal and LTL + proximal renal tubules; right panels: <t>WT1/NEPHRIN</t> + glomeruli. Scale bars: 25 µm.
    Rabbit Anti Ncam, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Abcam anti nrcam antibody neuronal marker
    Sorted PAX2 -null human nephron progenitors undergo mesenchymal-to-epithelial transition. ( A ) LHX1 + renal vesicles are induced from both the wild-type and PAX2-null nephron progenitors at day 3 of co-culture with spinal cord. ( B ) E-CADHERIN (ECAD) + distal and CADHERIN6 (CDH6) + proximal renal tubules, as well as <t>NCAM</t> + S-shaped bodies, are formed from the wild-type and PAX2 -null nephron progenitors at day 6. ( C ) Renal tubules and glomeruli are formed at day 9. Left panels: ECAD + distal and LTL + proximal renal tubules; right panels: <t>WT1/NEPHRIN</t> + glomeruli. Scale bars: 25 µm.
    Anti Nrcam Antibody Neuronal Marker, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    NrCAM associates with Npn-2 through the TARNER sequence in Ig1. A , Postulated mechanism of NrCAM/Npn-2/PlexA3 as a receptor complex for Sema3F in inducing dendritic spine retraction in cortical pyramidal neurons. The sequence TARNER within the Ig1 domain

    Journal: The Journal of Neuroscience

    Article Title: Neural Cell Adhesion Molecule NrCAM Regulates Semaphorin 3F-Induced Dendritic Spine Remodeling

    doi: 10.1523/JNEUROSCI.1774-14.2014

    Figure Lengend Snippet: NrCAM associates with Npn-2 through the TARNER sequence in Ig1. A , Postulated mechanism of NrCAM/Npn-2/PlexA3 as a receptor complex for Sema3F in inducing dendritic spine retraction in cortical pyramidal neurons. The sequence TARNER within the Ig1 domain

    Article Snippet: Polyclonal antibodies were as follows: GFP, NrCAM, and PlexA3 (Abcam); Npn-2 (R & D Systems); and NR1 (Santa Cruz Biotechnology).

    Techniques: Sequencing

    Cell binding and Neurocan interaction with NrCAM. (A) COS-7 cells transfected with vector alone (pCAGGS-IRES-mEGFP) or pCAGGS-NrCAM-IRES-mEGFP were pre-treated with 8 nM Neurocan, then fixed and subjected to immunofluorescence staining without permeabilization to detect surface-bound Neurocan (red). Scale bar = 100 μm. (B) Mean fluorescence intensity (±SEM) of Neurocan immunofluorescence staining on the surface of COS-7 cells, as shown in panel A . NrCAM-expressing cells treated with Neurocan showed significantly greater levels of bound Neurocan than untreated cells. Fluorescence intensity in cells with vector alone treated with Fc or Sema3F-Fc was non-significant (ns). ∗ p > 0.05, t -test, n = 5 images each condition. (C) Lysates (50 μg) of cells transfected with vector alone or pCAGGS-NrCAM-IRES-mEGFP were treated with Neurocan as in panel A , and immunoblotted (IB) with Neurocan antibodies. Blots were reprobed with antibodies directed against GAPDH (loading control) or NrCAM (expression control). Representative immunoblots of three experiments are shown. (D) Mouse cortical neuron cultures from NrCAM null mice were transfected with vector alone or pCAGGS-NrCAM-IRES-EGFP, and pre-treated with 20 nM Neurocan before fixation and immunostaining to detect surface-bound Neurocan. In merged images of EGFP (green) and Neurocan (red), more Neurocan immunofluorescence was observed on the surface of neurons expressing NrCAM than on NrCAM null neurons with vector alone. Scale bar = 50 μm. (E) Mean fluorescence intensity (±SEM) of surface-bound Neurocan immunostaining on neurons in panel D NrCAM-expressing cells treated with Neurocan showed significantly greater levels of bound Neurocan than NrCAM-minus neurons. ∗ p > 0.05, t -test, and n = 10 neurons per condition. (F) ELISA of Neurocan-AP or control AP protein binding to NrCAM-Fc or positive control NCAM-Fc on protein A-coated microtiter wells. AP binding was detected colorimetrically with p-nitrophenylphosphate. The mean (±SEM) optical densities (OD 405) of Neurocan-AP bound to NrCAM-Fc or NCAM-Fc were significantly greater than control AP ( t -test and ∗ p > 0.05). (G) Recombinant human Neurocan was incubated in Tris buffered saline with purified Fc, Sema3F-Fc, or Sema3A-Fc proteins, then complexes were pulled down with Protein A/G Sepharose beads. Immunoblotting for Neurocan showed no binding of Neurocan to Fc or Sema3F-Fc, whereas Neurocan bound effectively to Sema3A-Fc. Blots were reprobed with anti-Fc antibodies to demonstrate that equivalent amounts of Fc fusion proteins were pulled down. Recombinant Neurocan (left lane) ran as a broad band between 250 and 130 kDa.

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Neurocan Inhibits Semaphorin 3F Induced Dendritic Spine Remodeling Through NrCAM in Cortical Neurons

    doi: 10.3389/fncel.2018.00346

    Figure Lengend Snippet: Cell binding and Neurocan interaction with NrCAM. (A) COS-7 cells transfected with vector alone (pCAGGS-IRES-mEGFP) or pCAGGS-NrCAM-IRES-mEGFP were pre-treated with 8 nM Neurocan, then fixed and subjected to immunofluorescence staining without permeabilization to detect surface-bound Neurocan (red). Scale bar = 100 μm. (B) Mean fluorescence intensity (±SEM) of Neurocan immunofluorescence staining on the surface of COS-7 cells, as shown in panel A . NrCAM-expressing cells treated with Neurocan showed significantly greater levels of bound Neurocan than untreated cells. Fluorescence intensity in cells with vector alone treated with Fc or Sema3F-Fc was non-significant (ns). ∗ p > 0.05, t -test, n = 5 images each condition. (C) Lysates (50 μg) of cells transfected with vector alone or pCAGGS-NrCAM-IRES-mEGFP were treated with Neurocan as in panel A , and immunoblotted (IB) with Neurocan antibodies. Blots were reprobed with antibodies directed against GAPDH (loading control) or NrCAM (expression control). Representative immunoblots of three experiments are shown. (D) Mouse cortical neuron cultures from NrCAM null mice were transfected with vector alone or pCAGGS-NrCAM-IRES-EGFP, and pre-treated with 20 nM Neurocan before fixation and immunostaining to detect surface-bound Neurocan. In merged images of EGFP (green) and Neurocan (red), more Neurocan immunofluorescence was observed on the surface of neurons expressing NrCAM than on NrCAM null neurons with vector alone. Scale bar = 50 μm. (E) Mean fluorescence intensity (±SEM) of surface-bound Neurocan immunostaining on neurons in panel D NrCAM-expressing cells treated with Neurocan showed significantly greater levels of bound Neurocan than NrCAM-minus neurons. ∗ p > 0.05, t -test, and n = 10 neurons per condition. (F) ELISA of Neurocan-AP or control AP protein binding to NrCAM-Fc or positive control NCAM-Fc on protein A-coated microtiter wells. AP binding was detected colorimetrically with p-nitrophenylphosphate. The mean (±SEM) optical densities (OD 405) of Neurocan-AP bound to NrCAM-Fc or NCAM-Fc were significantly greater than control AP ( t -test and ∗ p > 0.05). (G) Recombinant human Neurocan was incubated in Tris buffered saline with purified Fc, Sema3F-Fc, or Sema3A-Fc proteins, then complexes were pulled down with Protein A/G Sepharose beads. Immunoblotting for Neurocan showed no binding of Neurocan to Fc or Sema3F-Fc, whereas Neurocan bound effectively to Sema3A-Fc. Blots were reprobed with anti-Fc antibodies to demonstrate that equivalent amounts of Fc fusion proteins were pulled down. Recombinant Neurocan (left lane) ran as a broad band between 250 and 130 kDa.

    Article Snippet: Lysates (50 μg) were subjected to Western blotting with the following antibodies: anti-NrCAM (1:1000, Abcam), anti-Neurocan (1:500, R & D), anti-Sema3F (1:500, Millipore), and anti-β actin (1:1000, Millipore).

    Techniques: Binding Assay, Transfection, Plasmid Preparation, Immunofluorescence, Staining, Fluorescence, Expressing, Western Blot, Mouse Assay, Immunostaining, Enzyme-linked Immunosorbent Assay, Protein Binding, Positive Control, Recombinant, Incubation, Purification

    Sorted PAX2 -null human nephron progenitors undergo mesenchymal-to-epithelial transition. ( A ) LHX1 + renal vesicles are induced from both the wild-type and PAX2-null nephron progenitors at day 3 of co-culture with spinal cord. ( B ) E-CADHERIN (ECAD) + distal and CADHERIN6 (CDH6) + proximal renal tubules, as well as NCAM + S-shaped bodies, are formed from the wild-type and PAX2 -null nephron progenitors at day 6. ( C ) Renal tubules and glomeruli are formed at day 9. Left panels: ECAD + distal and LTL + proximal renal tubules; right panels: WT1/NEPHRIN + glomeruli. Scale bars: 25 µm.

    Journal: Scientific Reports

    Article Title: PAX2 is dispensable for in vitro nephron formation from human induced pluripotent stem cells

    doi: 10.1038/s41598-017-04813-3

    Figure Lengend Snippet: Sorted PAX2 -null human nephron progenitors undergo mesenchymal-to-epithelial transition. ( A ) LHX1 + renal vesicles are induced from both the wild-type and PAX2-null nephron progenitors at day 3 of co-culture with spinal cord. ( B ) E-CADHERIN (ECAD) + distal and CADHERIN6 (CDH6) + proximal renal tubules, as well as NCAM + S-shaped bodies, are formed from the wild-type and PAX2 -null nephron progenitors at day 6. ( C ) Renal tubules and glomeruli are formed at day 9. Left panels: ECAD + distal and LTL + proximal renal tubules; right panels: WT1/NEPHRIN + glomeruli. Scale bars: 25 µm.

    Article Snippet: The following primary antibodies were used: rabbit anti-PAX2 (Biolegend; 901001); mouse anti-turboGFP (Origene; TA150041); rabbit anti-WT1 (C19) (Santa Cruz Biotechnology; sc-192); anti-LHX1 (Developmental Studies Hybridoma Bank; 4F2); mouse anti-E-cadherin (BD Biosciences; 610181); rabbit anti-cadherin6 (a kind gift from Dr. Gregory Dressler, University of Michigan) ; mouse anti-NCAM (Ancell; 208-020); rabbit anti-NCAM (Millipore; AB5032); guinea pig anti-nephrin (Progen; GP-N2); goat anti-podocalyxin (R & D Systems; AF1658); anti-rat anti-cytokeratin-8 (Developmental Studies Hybridoma Bank; TROMA-1); mouse anti-SALL1 (Perseus Proteomics; PP-K9814-00); and rabbit anti-phosphorylated aPKC (Abcam; ab62372).

    Techniques: Co-Culture Assay