nox 2 primary antibody Search Results


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  • 93
    Bio-Techne corporation nbp2 41291
    Nbp2 41291, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nbp2 41291/product/Bio-Techne corporation
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nbp2 41291 - by Bioz Stars, 2024-05
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    93
    Bioss nox2/gp91phox polyclonal antibody
    Nox2/Gp91phox Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nox2/gp91phox polyclonal antibody/product/Bioss
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nox2/gp91phox polyclonal antibody - by Bioz Stars, 2024-05
    93/100 stars
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    94
    Proteintech nox2
    ROS generation and phosphorylation of VASP, ERK1/2, p38 MAPK, ERK5, JNK, AKT and c-PLA2. (A) Western blot analysis of the expression of <t>NOX2,</t> p67 phox , NOX1, NOXO1 and Rac in WT and p47 phox -/- platelets. (B) ROS generation in platelets after stimulation with CRP (2 μg/ml) or thrombin (0.5 U/ml) was expressed as mean fluorescent intensity (MFI) (mean ± SE, n = 6) (Student t-test). (C) The phosphorylation level of VASP, ERK1/2, p38, ERK5 and JNK in CRP-stimulated platelets was detected by western blot and (D) quantified as a ratio relative to the total level (mean ± SD, n = 3) (Two-way ANOVA). (E) The phosphorylation level of AKT and c-PLA2 was also detected and (F) quantified (mean ± SD, n = 3) (Two-way ANOVA). *p < 0.05; **p < 0.01; ***p < 0.001.
    Nox2, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nox2/product/Proteintech
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nox2 - by Bioz Stars, 2024-05
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    94
    Proteintech anti gp91 phox
    ROS generation and phosphorylation of VASP, ERK1/2, p38 MAPK, ERK5, JNK, AKT and c-PLA2. (A) Western blot analysis of the expression of <t>NOX2,</t> p67 phox , NOX1, NOXO1 and Rac in WT and p47 phox -/- platelets. (B) ROS generation in platelets after stimulation with CRP (2 μg/ml) or thrombin (0.5 U/ml) was expressed as mean fluorescent intensity (MFI) (mean ± SE, n = 6) (Student t-test). (C) The phosphorylation level of VASP, ERK1/2, p38, ERK5 and JNK in CRP-stimulated platelets was detected by western blot and (D) quantified as a ratio relative to the total level (mean ± SD, n = 3) (Two-way ANOVA). (E) The phosphorylation level of AKT and c-PLA2 was also detected and (F) quantified (mean ± SD, n = 3) (Two-way ANOVA). *p < 0.05; **p < 0.01; ***p < 0.001.
    Anti Gp91 Phox, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti gp91 phox/product/Proteintech
    Average 94 stars, based on 1 article reviews
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    anti gp91 phox - by Bioz Stars, 2024-05
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    94
    Proteintech nadphoxidase 1
    ROS generation and phosphorylation of VASP, ERK1/2, p38 MAPK, ERK5, JNK, AKT and c-PLA2. (A) Western blot analysis of the expression of <t>NOX2,</t> p67 phox , NOX1, NOXO1 and Rac in WT and p47 phox -/- platelets. (B) ROS generation in platelets after stimulation with CRP (2 μg/ml) or thrombin (0.5 U/ml) was expressed as mean fluorescent intensity (MFI) (mean ± SE, n = 6) (Student t-test). (C) The phosphorylation level of VASP, ERK1/2, p38, ERK5 and JNK in CRP-stimulated platelets was detected by western blot and (D) quantified as a ratio relative to the total level (mean ± SD, n = 3) (Two-way ANOVA). (E) The phosphorylation level of AKT and c-PLA2 was also detected and (F) quantified (mean ± SD, n = 3) (Two-way ANOVA). *p < 0.05; **p < 0.01; ***p < 0.001.
    Nadphoxidase 1, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nadphoxidase 1/product/Proteintech
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nadphoxidase 1 - by Bioz Stars, 2024-05
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    Image Search Results


    ROS generation and phosphorylation of VASP, ERK1/2, p38 MAPK, ERK5, JNK, AKT and c-PLA2. (A) Western blot analysis of the expression of NOX2, p67 phox , NOX1, NOXO1 and Rac in WT and p47 phox -/- platelets. (B) ROS generation in platelets after stimulation with CRP (2 μg/ml) or thrombin (0.5 U/ml) was expressed as mean fluorescent intensity (MFI) (mean ± SE, n = 6) (Student t-test). (C) The phosphorylation level of VASP, ERK1/2, p38, ERK5 and JNK in CRP-stimulated platelets was detected by western blot and (D) quantified as a ratio relative to the total level (mean ± SD, n = 3) (Two-way ANOVA). (E) The phosphorylation level of AKT and c-PLA2 was also detected and (F) quantified (mean ± SD, n = 3) (Two-way ANOVA). *p < 0.05; **p < 0.01; ***p < 0.001.

    Journal: Redox Biology

    Article Title: p47 phox deficiency impairs platelet function and protects mice against arterial and venous thrombosis

    doi: 10.1016/j.redox.2020.101569

    Figure Lengend Snippet: ROS generation and phosphorylation of VASP, ERK1/2, p38 MAPK, ERK5, JNK, AKT and c-PLA2. (A) Western blot analysis of the expression of NOX2, p67 phox , NOX1, NOXO1 and Rac in WT and p47 phox -/- platelets. (B) ROS generation in platelets after stimulation with CRP (2 μg/ml) or thrombin (0.5 U/ml) was expressed as mean fluorescent intensity (MFI) (mean ± SE, n = 6) (Student t-test). (C) The phosphorylation level of VASP, ERK1/2, p38, ERK5 and JNK in CRP-stimulated platelets was detected by western blot and (D) quantified as a ratio relative to the total level (mean ± SD, n = 3) (Two-way ANOVA). (E) The phosphorylation level of AKT and c-PLA2 was also detected and (F) quantified (mean ± SD, n = 3) (Two-way ANOVA). *p < 0.05; **p < 0.01; ***p < 0.001.

    Article Snippet: Immunoblotting assay involves antibodies against p47 phox (Santa Cruz Biotechnology); NOX1 and NOX2 (Proteintech); p67 phox (Abcam); NOXO1 (ABclonal Technology); Rac (Santa Cruz Biotechnology); Syk (anti-Tyr-525 and pan-Syk, Bioworld Technology); PLCγ2 (anti-Tyr-1217 and pan-PLCγ2; Bioworld Technology); VASP (anti-Ser157, Affinity Biosciences; anti-Ser239 and pan-VASP, Cell Signaling Technology); ERK1/2 (anti-Thr202/Tyr204 and pan-ERK1/2, Cell Signaling Technology); p38 MAPK (anti-Thr180/Tyr182, Cell Signaling Technology); ERK5 (anti-Thr218/Tyr220, Cell Signaling Technology; pan-ERK5, Proteintech); JNK (anti-Thr183/Tyr185, Cell Signaling Technology; pan-JNK, Affinity Biosciences); AKT (anti-Thr308, Cell Signaling Technology; pan-AKT, Affinity Biosciences); c-PLA2 (anti-Ser505 and pan-c-PLA2, Affinity Biosciences); NOX1 (Novus Biologicals); NOX2 (Abcam).

    Techniques: Western Blot, Expressing

    p47 phox translocation and interaction with NOX1 and NOX2. (A) Platelets were treated with CRP (5 μg/ml) for 30s or thrombin (1 U/ml) for 60s followed by isolation of the membrane and cytosol to measure the expression of p47 phox and (B) p47 phox expression was quantified as a ratio relative to the internal control β3 or GAPDH. Data were presented as mean ± SD (n = 3). Compared to corresponding 0, *p < 0.05. (C) Immunoprecipitation analysis of the interaction of p47 phox with NOX2 or NOX1 in platelets after stimulation with CRP (5 μg/ml) or thrombin (1 U/ml). Compared to resting, *p < 0.05; **p < 0.01; ***p < 0.001. (D) ROS generation in p47 phox -/- platelets in the presence of ML171 (5 μM) (NOX1 inhibitor) and/or gp91ds-at (50 μM) (NOX2 inhibitor) after CRP (2 μg/ml) or thrombin (0.5 U/ml) stimulation. (E) Platelet aggregation in p47 phox -/- platelets in the presence of ML171 (5 μM) (NOX1 inhibitor) and/or gp91ds-at (50 μM) (NOX2 inhibitor) after CRP (0.25 μg/ml) stimulation. Data were presented as mean ± SE (n = 6–8) (One-way ANOVA). Compared to WT, *p < 0.05; **p < 0.01. Compared with p47 phox -/- , &p < 0.01.

    Journal: Redox Biology

    Article Title: p47 phox deficiency impairs platelet function and protects mice against arterial and venous thrombosis

    doi: 10.1016/j.redox.2020.101569

    Figure Lengend Snippet: p47 phox translocation and interaction with NOX1 and NOX2. (A) Platelets were treated with CRP (5 μg/ml) for 30s or thrombin (1 U/ml) for 60s followed by isolation of the membrane and cytosol to measure the expression of p47 phox and (B) p47 phox expression was quantified as a ratio relative to the internal control β3 or GAPDH. Data were presented as mean ± SD (n = 3). Compared to corresponding 0, *p < 0.05. (C) Immunoprecipitation analysis of the interaction of p47 phox with NOX2 or NOX1 in platelets after stimulation with CRP (5 μg/ml) or thrombin (1 U/ml). Compared to resting, *p < 0.05; **p < 0.01; ***p < 0.001. (D) ROS generation in p47 phox -/- platelets in the presence of ML171 (5 μM) (NOX1 inhibitor) and/or gp91ds-at (50 μM) (NOX2 inhibitor) after CRP (2 μg/ml) or thrombin (0.5 U/ml) stimulation. (E) Platelet aggregation in p47 phox -/- platelets in the presence of ML171 (5 μM) (NOX1 inhibitor) and/or gp91ds-at (50 μM) (NOX2 inhibitor) after CRP (0.25 μg/ml) stimulation. Data were presented as mean ± SE (n = 6–8) (One-way ANOVA). Compared to WT, *p < 0.05; **p < 0.01. Compared with p47 phox -/- , &p < 0.01.

    Article Snippet: Immunoblotting assay involves antibodies against p47 phox (Santa Cruz Biotechnology); NOX1 and NOX2 (Proteintech); p67 phox (Abcam); NOXO1 (ABclonal Technology); Rac (Santa Cruz Biotechnology); Syk (anti-Tyr-525 and pan-Syk, Bioworld Technology); PLCγ2 (anti-Tyr-1217 and pan-PLCγ2; Bioworld Technology); VASP (anti-Ser157, Affinity Biosciences; anti-Ser239 and pan-VASP, Cell Signaling Technology); ERK1/2 (anti-Thr202/Tyr204 and pan-ERK1/2, Cell Signaling Technology); p38 MAPK (anti-Thr180/Tyr182, Cell Signaling Technology); ERK5 (anti-Thr218/Tyr220, Cell Signaling Technology; pan-ERK5, Proteintech); JNK (anti-Thr183/Tyr185, Cell Signaling Technology; pan-JNK, Affinity Biosciences); AKT (anti-Thr308, Cell Signaling Technology; pan-AKT, Affinity Biosciences); c-PLA2 (anti-Ser505 and pan-c-PLA2, Affinity Biosciences); NOX1 (Novus Biologicals); NOX2 (Abcam).

    Techniques: Translocation Assay, Isolation, Expressing, Immunoprecipitation