novablue Millipore Search Results


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  • 97
    Millipore coli novablue
    Coli Novablue, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Millipore novablue
    Loss of recoverable pETBlue plasmid DNA from transformed <t>NovaBlue</t> cells following incubation with HOCl. Conditions: ~1×10 8 E. coli /mL in 10 mL PBS incubated at 37 °C with the indicated amounts of HOCl for 4 h, after which a 10-fold excess of Na 2 S 2 O 3 was added to remove remaining oxidant, the plasmid was isolated, resuspended in H 2 O, and run on 0.8% agarose as described in Experimental Procedures. Lane 1 shows purified pETBlue that had been digested with EcoR1, a restriction enzyme which catalyzes a single double-strand cut in the plasmid. Lane 7 contains the 1 kB DNA ladder. No DNA was recovered from cells exposed to HOCl at higher concentrations ([HOCl] = 250–1000 µM).
    Novablue, supplied by Millipore, used in various techniques. Bioz Stars score: 89/100, based on 245 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Millipore novablue de3
    Loss of recoverable pETBlue plasmid DNA from transformed <t>NovaBlue</t> cells following incubation with HOCl. Conditions: ~1×10 8 E. coli /mL in 10 mL PBS incubated at 37 °C with the indicated amounts of HOCl for 4 h, after which a 10-fold excess of Na 2 S 2 O 3 was added to remove remaining oxidant, the plasmid was isolated, resuspended in H 2 O, and run on 0.8% agarose as described in Experimental Procedures. Lane 1 shows purified pETBlue that had been digested with EcoR1, a restriction enzyme which catalyzes a single double-strand cut in the plasmid. Lane 7 contains the 1 kB DNA ladder. No DNA was recovered from cells exposed to HOCl at higher concentrations ([HOCl] = 250–1000 µM).
    Novablue De3, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    novablue de3 - by Bioz Stars, 2020-08
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    85
    Millipore amplification host novablue
    Loss of recoverable pETBlue plasmid DNA from transformed <t>NovaBlue</t> cells following incubation with HOCl. Conditions: ~1×10 8 E. coli /mL in 10 mL PBS incubated at 37 °C with the indicated amounts of HOCl for 4 h, after which a 10-fold excess of Na 2 S 2 O 3 was added to remove remaining oxidant, the plasmid was isolated, resuspended in H 2 O, and run on 0.8% agarose as described in Experimental Procedures. Lane 1 shows purified pETBlue that had been digested with EcoR1, a restriction enzyme which catalyzes a single double-strand cut in the plasmid. Lane 7 contains the 1 kB DNA ladder. No DNA was recovered from cells exposed to HOCl at higher concentrations ([HOCl] = 250–1000 µM).
    Amplification Host Novablue, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Millipore novablue singles competent cells
    Loss of recoverable pETBlue plasmid DNA from transformed <t>NovaBlue</t> cells following incubation with HOCl. Conditions: ~1×10 8 E. coli /mL in 10 mL PBS incubated at 37 °C with the indicated amounts of HOCl for 4 h, after which a 10-fold excess of Na 2 S 2 O 3 was added to remove remaining oxidant, the plasmid was isolated, resuspended in H 2 O, and run on 0.8% agarose as described in Experimental Procedures. Lane 1 shows purified pETBlue that had been digested with EcoR1, a restriction enzyme which catalyzes a single double-strand cut in the plasmid. Lane 7 contains the 1 kB DNA ladder. No DNA was recovered from cells exposed to HOCl at higher concentrations ([HOCl] = 250–1000 µM).
    Novablue Singles Competent Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 239 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    novablue singles competent cells - by Bioz Stars, 2020-08
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    92
    Millipore novablue ultracompetent cells
    Loss of recoverable pETBlue plasmid DNA from transformed <t>NovaBlue</t> cells following incubation with HOCl. Conditions: ~1×10 8 E. coli /mL in 10 mL PBS incubated at 37 °C with the indicated amounts of HOCl for 4 h, after which a 10-fold excess of Na 2 S 2 O 3 was added to remove remaining oxidant, the plasmid was isolated, resuspended in H 2 O, and run on 0.8% agarose as described in Experimental Procedures. Lane 1 shows purified pETBlue that had been digested with EcoR1, a restriction enzyme which catalyzes a single double-strand cut in the plasmid. Lane 7 contains the 1 kB DNA ladder. No DNA was recovered from cells exposed to HOCl at higher concentrations ([HOCl] = 250–1000 µM).
    Novablue Ultracompetent Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Millipore novablue gigasingles competent cells
    Loss of recoverable pETBlue plasmid DNA from transformed <t>NovaBlue</t> cells following incubation with HOCl. Conditions: ~1×10 8 E. coli /mL in 10 mL PBS incubated at 37 °C with the indicated amounts of HOCl for 4 h, after which a 10-fold excess of Na 2 S 2 O 3 was added to remove remaining oxidant, the plasmid was isolated, resuspended in H 2 O, and run on 0.8% agarose as described in Experimental Procedures. Lane 1 shows purified pETBlue that had been digested with EcoR1, a restriction enzyme which catalyzes a single double-strand cut in the plasmid. Lane 7 contains the 1 kB DNA ladder. No DNA was recovered from cells exposed to HOCl at higher concentrations ([HOCl] = 250–1000 µM).
    Novablue Gigasingles Competent Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 135 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Millipore novablue gigasingles
    Loss of recoverable pETBlue plasmid DNA from transformed <t>NovaBlue</t> cells following incubation with HOCl. Conditions: ~1×10 8 E. coli /mL in 10 mL PBS incubated at 37 °C with the indicated amounts of HOCl for 4 h, after which a 10-fold excess of Na 2 S 2 O 3 was added to remove remaining oxidant, the plasmid was isolated, resuspended in H 2 O, and run on 0.8% agarose as described in Experimental Procedures. Lane 1 shows purified pETBlue that had been digested with EcoR1, a restriction enzyme which catalyzes a single double-strand cut in the plasmid. Lane 7 contains the 1 kB DNA ladder. No DNA was recovered from cells exposed to HOCl at higher concentrations ([HOCl] = 250–1000 µM).
    Novablue Gigasingles, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Millipore novablue novagen electrocompetent cells
    Loss of recoverable pETBlue plasmid DNA from transformed <t>NovaBlue</t> cells following incubation with HOCl. Conditions: ~1×10 8 E. coli /mL in 10 mL PBS incubated at 37 °C with the indicated amounts of HOCl for 4 h, after which a 10-fold excess of Na 2 S 2 O 3 was added to remove remaining oxidant, the plasmid was isolated, resuspended in H 2 O, and run on 0.8% agarose as described in Experimental Procedures. Lane 1 shows purified pETBlue that had been digested with EcoR1, a restriction enzyme which catalyzes a single double-strand cut in the plasmid. Lane 7 contains the 1 kB DNA ladder. No DNA was recovered from cells exposed to HOCl at higher concentrations ([HOCl] = 250–1000 µM).
    Novablue Novagen Electrocompetent Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Millipore novablue λde3
    Loss of recoverable pETBlue plasmid DNA from transformed <t>NovaBlue</t> cells following incubation with HOCl. Conditions: ~1×10 8 E. coli /mL in 10 mL PBS incubated at 37 °C with the indicated amounts of HOCl for 4 h, after which a 10-fold excess of Na 2 S 2 O 3 was added to remove remaining oxidant, the plasmid was isolated, resuspended in H 2 O, and run on 0.8% agarose as described in Experimental Procedures. Lane 1 shows purified pETBlue that had been digested with EcoR1, a restriction enzyme which catalyzes a single double-strand cut in the plasmid. Lane 7 contains the 1 kB DNA ladder. No DNA was recovered from cells exposed to HOCl at higher concentrations ([HOCl] = 250–1000 µM).
    Novablue λde3, supplied by Millipore, used in various techniques. Bioz Stars score: 80/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Millipore e scherichia coli novablue de3 competent cell
    Loss of recoverable pETBlue plasmid DNA from transformed <t>NovaBlue</t> cells following incubation with HOCl. Conditions: ~1×10 8 E. coli /mL in 10 mL PBS incubated at 37 °C with the indicated amounts of HOCl for 4 h, after which a 10-fold excess of Na 2 S 2 O 3 was added to remove remaining oxidant, the plasmid was isolated, resuspended in H 2 O, and run on 0.8% agarose as described in Experimental Procedures. Lane 1 shows purified pETBlue that had been digested with EcoR1, a restriction enzyme which catalyzes a single double-strand cut in the plasmid. Lane 7 contains the 1 kB DNA ladder. No DNA was recovered from cells exposed to HOCl at higher concentrations ([HOCl] = 250–1000 µM).
    E Scherichia Coli Novablue De3 Competent Cell, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore competent e coli
    Loss of recoverable pETBlue plasmid DNA from transformed <t>NovaBlue</t> cells following incubation with HOCl. Conditions: ~1×10 8 E. coli /mL in 10 mL PBS incubated at 37 °C with the indicated amounts of HOCl for 4 h, after which a 10-fold excess of Na 2 S 2 O 3 was added to remove remaining oxidant, the plasmid was isolated, resuspended in H 2 O, and run on 0.8% agarose as described in Experimental Procedures. Lane 1 shows purified pETBlue that had been digested with EcoR1, a restriction enzyme which catalyzes a single double-strand cut in the plasmid. Lane 7 contains the 1 kB DNA ladder. No DNA was recovered from cells exposed to HOCl at higher concentrations ([HOCl] = 250–1000 µM).
    Competent E Coli, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 214 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Millipore novablue gigasinglestm
    Loss of recoverable pETBlue plasmid DNA from transformed <t>NovaBlue</t> cells following incubation with HOCl. Conditions: ~1×10 8 E. coli /mL in 10 mL PBS incubated at 37 °C with the indicated amounts of HOCl for 4 h, after which a 10-fold excess of Na 2 S 2 O 3 was added to remove remaining oxidant, the plasmid was isolated, resuspended in H 2 O, and run on 0.8% agarose as described in Experimental Procedures. Lane 1 shows purified pETBlue that had been digested with EcoR1, a restriction enzyme which catalyzes a single double-strand cut in the plasmid. Lane 7 contains the 1 kB DNA ladder. No DNA was recovered from cells exposed to HOCl at higher concentrations ([HOCl] = 250–1000 µM).
    Novablue Gigasinglestm, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Loss of recoverable pETBlue plasmid DNA from transformed NovaBlue cells following incubation with HOCl. Conditions: ~1×10 8 E. coli /mL in 10 mL PBS incubated at 37 °C with the indicated amounts of HOCl for 4 h, after which a 10-fold excess of Na 2 S 2 O 3 was added to remove remaining oxidant, the plasmid was isolated, resuspended in H 2 O, and run on 0.8% agarose as described in Experimental Procedures. Lane 1 shows purified pETBlue that had been digested with EcoR1, a restriction enzyme which catalyzes a single double-strand cut in the plasmid. Lane 7 contains the 1 kB DNA ladder. No DNA was recovered from cells exposed to HOCl at higher concentrations ([HOCl] = 250–1000 µM).

    Journal: Archives of biochemistry and biophysics

    Article Title: Comparative study of HOCl-inflicted damage to bacterial DNA ex vivo and within cells

    doi: 10.1016/j.abb.2009.10.006

    Figure Lengend Snippet: Loss of recoverable pETBlue plasmid DNA from transformed NovaBlue cells following incubation with HOCl. Conditions: ~1×10 8 E. coli /mL in 10 mL PBS incubated at 37 °C with the indicated amounts of HOCl for 4 h, after which a 10-fold excess of Na 2 S 2 O 3 was added to remove remaining oxidant, the plasmid was isolated, resuspended in H 2 O, and run on 0.8% agarose as described in Experimental Procedures. Lane 1 shows purified pETBlue that had been digested with EcoR1, a restriction enzyme which catalyzes a single double-strand cut in the plasmid. Lane 7 contains the 1 kB DNA ladder. No DNA was recovered from cells exposed to HOCl at higher concentrations ([HOCl] = 250–1000 µM).

    Article Snippet: Biological); Hoechst 33342 trihydrochloride trihydrate (Molecular Probes); pETBlue plasmid and NovaBlue cells (Novagen).

    Techniques: Plasmid Preparation, Transformation Assay, Incubation, Isolation, Purification

    Reaction of plasmid DNA in pETBlue-transformed NovaBlue cells exposed to ONOOH. Conditions: ~3×10 9 cells/mL in 50 mM phosphate, pH 7.4, under an argon atmosphere mixed with the indicated amounts of peroxynitrite at ambient temperature, after which the plasmid was isolated and run on 0.8% agarose.

    Journal: Archives of biochemistry and biophysics

    Article Title: Comparative study of HOCl-inflicted damage to bacterial DNA ex vivo and within cells

    doi: 10.1016/j.abb.2009.10.006

    Figure Lengend Snippet: Reaction of plasmid DNA in pETBlue-transformed NovaBlue cells exposed to ONOOH. Conditions: ~3×10 9 cells/mL in 50 mM phosphate, pH 7.4, under an argon atmosphere mixed with the indicated amounts of peroxynitrite at ambient temperature, after which the plasmid was isolated and run on 0.8% agarose.

    Article Snippet: Biological); Hoechst 33342 trihydrochloride trihydrate (Molecular Probes); pETBlue plasmid and NovaBlue cells (Novagen).

    Techniques: Plasmid Preparation, Transformation Assay, Isolation

    Degradation of DNA in NovaBlue cells following exposure to HOCl. Conditions: E. coli suspended at the indicated cell densities in 50 mM phosphate, pH 7.4, incubated 15 min at ambient temperature, then oxidant quenched with excess Na 2 S 2 O 3 , and the DNA isolated and run on 0.8% agarose. Panel a: 2.4×10 8 plasmid-free cells/mL, for which LD 90 ~38 µM (color photograph) ; panel b: 5×10 7 pETBlue transformed cells/mL, for which LD 90 ~8 µM.

    Journal: Archives of biochemistry and biophysics

    Article Title: Comparative study of HOCl-inflicted damage to bacterial DNA ex vivo and within cells

    doi: 10.1016/j.abb.2009.10.006

    Figure Lengend Snippet: Degradation of DNA in NovaBlue cells following exposure to HOCl. Conditions: E. coli suspended at the indicated cell densities in 50 mM phosphate, pH 7.4, incubated 15 min at ambient temperature, then oxidant quenched with excess Na 2 S 2 O 3 , and the DNA isolated and run on 0.8% agarose. Panel a: 2.4×10 8 plasmid-free cells/mL, for which LD 90 ~38 µM (color photograph) ; panel b: 5×10 7 pETBlue transformed cells/mL, for which LD 90 ~8 µM.

    Article Snippet: Biological); Hoechst 33342 trihydrochloride trihydrate (Molecular Probes); pETBlue plasmid and NovaBlue cells (Novagen).

    Techniques: Incubation, Isolation, Plasmid Preparation, Transformation Assay