Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

Article Title: A liquid chromatography with tandem mass spectrometry method for simultaneous determination of UTL-5g and its metabolites in human plasma

doi: 10.1016/j.jchromb.2015.04.015

Figure Lengend Snippet: A total ion chromatogram of a standard mixture of UTL-5g (at m/z of 271.17 > 109.96), ISOX (at m/z of 128.05 > 109.96), DCA (at m/z of 161.92 > 125.95) and the internal standard zileuton (at m/z of 237.08 > 161.03) at the concentration of 1 μM. Chromatographic separation was performed on a Nova-Pak C18 column (4 μm, 3.9 mm × 150 mm) at 30°C, running with mobile phase consisting of 0.1% formic acid in water (A) and 0.1% formic acid in methanol (B) at the flow rate of 0.2 mL/min. Mobile phase gradient B%(min) was programmed as 10 (0) → 95(3.5) → 95 (19) → 10(19.5) → 10(24). The retention times were 16.86 ± 0.02 for UTL-5g, 11.85 ± 0.01 for ISOX and 14.60 ± 0.02 for DCA, and 13.53 ± 0.02 for internal standard zileuton.

Article Snippet: UTL-5g, its metabolites DCA and ISOX, and the internal standard zileuton were separated from potentially interfering material on a Nova-Pak C18 column (4 μm, 3.9 mm × 150 mm; Waters Corp., Milford, MA) maintained at 30 °C.

Techniques: Concentration Assay, Flow Cytometry

Journal: Advanced Biomedical Research

Article Title: Neuroprotective effects of Rosa damascena extract on learning and memory in a rat model of amyloid-β-induced Alzheimer's disease

doi: 10.4103/2277-9175.161512

Figure Lengend Snippet: (a) HPLC chromatogram of quercetin standard at 365 nm; 20 μL of the quercetin standard (Sigma Aldrich, USA) in the range of 100–1000 μg/mL was injected into a Nova-Pak C18, 3.9 × 150 mm (Waters, Milford, MA, USA) using H 3 PO 4 10 mM in water (solvent A) and acetonitrile (solvent B) with gradient elution at a flow rate of 0.8 mL/min. Quercetin peak appeared at a retention time of 13.64 min. (b) HPLC chromatogram of Rosa damascena extract at 365 nm. After acid hydrolysis of 100 mg of the extract (1 h in 2N HCl, at 95°C), the hydrolyzed flavonoids were extracted through ethyl acetate to 5 mL. Then 20 μL of the sample was injected into a Nova-Pak C18, 3.9 × 150 mm (Waters, Milford, MA, USA) using H 3 PO 4 10 mM in water (solvent A) and acetonitrile (solvent B) with gradient elution at a flow rate of 0.8 ml/min. Quercetin peak of the extract appeared at a retention time of 13.48 min. (c): Calibration curve of quercetin using HPLC method and acetonitrile/water as mobile phase with pH adjusted to 2.3 at 365 nm. Using the millennium processing software, the calibration curve was determined by linear regression in the range of 100–1000 μg/mL. The regression equation was y = 86,419 x + 7, 94,975

Article Snippet: Then 20 μl of the sample injected into a Nova-Pak C18, 3.9 × 150 mm (Waters, Milford, MA, USA) using H3 PO4 10 mM in water (solvent A) and acetonitrile (solvent B) with gradient elution at a flow rate of 0.8 mL/min.

Techniques: High Performance Liquid Chromatography, Injection, Flow Cytometry, Software

Journal:

Article Title: Irreversible Binding of a Novel Phenylisothiocyanate Tropane Analog to Monoamine Transporters In Rat Brain

doi: 10.1016/j.bcp.2007.04.019

Figure Lengend Snippet: Purification and pharmacological characterization of radiolabeled [ 125 I]HD-244 by HPLC. After reaction of HD-243 with 600 μCi of Na[ 125 I] with iodo-beads, samples were eluted on a C18 HPLC column using a linear gradient of 20 mM HEPES, pH 7.0

Article Snippet: Nova-Pak C18 HPLC column was obtained from Waters Chromatography (Milford, MA).

Techniques: Purification, High Performance Liquid Chromatography