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  • 99
    Thermo Fisher nonidet p 40
    Nonidet P 40, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2740 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore nonidet p40
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    Nacalai nonidet p 40
    Substrate specificity of NAAA in the presenceof different stimulators.Recombinant NAAA (0.3–3 μg of protein) was allowed toreact with the indicated  14 C-labeled NAEs at 100 μMin the presence or absence of 0.1% Nonidet P-40,
    Nonidet P 40, supplied by Nacalai, used in various techniques. Bioz Stars score: 93/100, based on 874 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Boehringer Mannheim nonidet p 40
    Substrate specificity of NAAA in the presenceof different stimulators.Recombinant NAAA (0.3–3 μg of protein) was allowed toreact with the indicated  14 C-labeled NAEs at 100 μMin the presence or absence of 0.1% Nonidet P-40,
    Nonidet P 40, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 646 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology nonidet p 40
    PGC-1α interacts with OGT. A , Fao cells were infected  with Ad-FLAG-PGC-1α and treated with insulin for 1 h prior to harvesting  and OGT co-immunoprecipitation ( IP ). Immunoprecipitates were  subjected to SDS-PAGE and immunoblotted ( IB ) for the presence of  PGC-1α. Membranes were then stripped and blotted for OGT. Immunoblots  are representative of three experiments.  B , Fao cells were infected  with Ad-FLAG-PGC-1α and treated with insulin for 1 h prior to harvesting  and FLAG co-immunoprecipitation. Immunoprecipitates were subjected to SDS-PAGE  and immunoblotted for the presence of OGT. Membranes were then stripped and  blotted for PGC-1α.  C , gel filtration chromatography (SMART  system, Superdex 200 column, phosphate-buffered saline, 1% Nonidet P-40  buffer) of lysates from rat liver incubated on ice for 30 min with either  normal IgG or anti-OGT antibodies (AL28). Fractions were subjected to SDS-PAGE  and blotted using anti-PGC-1α, anti-OGT (DM-17), and anti CPB. Data are  representative of two experiments.
    Nonidet P 40, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 570 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad nonidet p 40
    PGC-1α interacts with OGT. A , Fao cells were infected  with Ad-FLAG-PGC-1α and treated with insulin for 1 h prior to harvesting  and OGT co-immunoprecipitation ( IP ). Immunoprecipitates were  subjected to SDS-PAGE and immunoblotted ( IB ) for the presence of  PGC-1α. Membranes were then stripped and blotted for OGT. Immunoblots  are representative of three experiments.  B , Fao cells were infected  with Ad-FLAG-PGC-1α and treated with insulin for 1 h prior to harvesting  and FLAG co-immunoprecipitation. Immunoprecipitates were subjected to SDS-PAGE  and immunoblotted for the presence of OGT. Membranes were then stripped and  blotted for PGC-1α.  C , gel filtration chromatography (SMART  system, Superdex 200 column, phosphate-buffered saline, 1% Nonidet P-40  buffer) of lysates from rat liver incubated on ice for 30 min with either  normal IgG or anti-OGT antibodies (AL28). Fractions were subjected to SDS-PAGE  and blotted using anti-PGC-1α, anti-OGT (DM-17), and anti CPB. Data are  representative of two experiments.
    Nonidet P 40, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 331 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher nonidet p 40 lysis buffer
    PGC-1α interacts with OGT. A , Fao cells were infected  with Ad-FLAG-PGC-1α and treated with insulin for 1 h prior to harvesting  and OGT co-immunoprecipitation ( IP ). Immunoprecipitates were  subjected to SDS-PAGE and immunoblotted ( IB ) for the presence of  PGC-1α. Membranes were then stripped and blotted for OGT. Immunoblots  are representative of three experiments.  B , Fao cells were infected  with Ad-FLAG-PGC-1α and treated with insulin for 1 h prior to harvesting  and FLAG co-immunoprecipitation. Immunoprecipitates were subjected to SDS-PAGE  and immunoblotted for the presence of OGT. Membranes were then stripped and  blotted for PGC-1α.  C , gel filtration chromatography (SMART  system, Superdex 200 column, phosphate-buffered saline, 1% Nonidet P-40  buffer) of lysates from rat liver incubated on ice for 30 min with either  normal IgG or anti-OGT antibodies (AL28). Fractions were subjected to SDS-PAGE  and blotted using anti-PGC-1α, anti-OGT (DM-17), and anti CPB. Data are  representative of two experiments.
    Nonidet P 40 Lysis Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 279 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Beyotime nonidet p 40
    PGC-1α interacts with OGT. A , Fao cells were infected  with Ad-FLAG-PGC-1α and treated with insulin for 1 h prior to harvesting  and OGT co-immunoprecipitation ( IP ). Immunoprecipitates were  subjected to SDS-PAGE and immunoblotted ( IB ) for the presence of  PGC-1α. Membranes were then stripped and blotted for OGT. Immunoblots  are representative of three experiments.  B , Fao cells were infected  with Ad-FLAG-PGC-1α and treated with insulin for 1 h prior to harvesting  and FLAG co-immunoprecipitation. Immunoprecipitates were subjected to SDS-PAGE  and immunoblotted for the presence of OGT. Membranes were then stripped and  blotted for PGC-1α.  C , gel filtration chromatography (SMART  system, Superdex 200 column, phosphate-buffered saline, 1% Nonidet P-40  buffer) of lysates from rat liver incubated on ice for 30 min with either  normal IgG or anti-OGT antibodies (AL28). Fractions were subjected to SDS-PAGE  and blotted using anti-PGC-1α, anti-OGT (DM-17), and anti CPB. Data are  representative of two experiments.
    Nonidet P 40, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 210 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore np 40 substitute
    PGC-1α interacts with OGT. A , Fao cells were infected  with Ad-FLAG-PGC-1α and treated with insulin for 1 h prior to harvesting  and OGT co-immunoprecipitation ( IP ). Immunoprecipitates were  subjected to SDS-PAGE and immunoblotted ( IB ) for the presence of  PGC-1α. Membranes were then stripped and blotted for OGT. Immunoblots  are representative of three experiments.  B , Fao cells were infected  with Ad-FLAG-PGC-1α and treated with insulin for 1 h prior to harvesting  and FLAG co-immunoprecipitation. Immunoprecipitates were subjected to SDS-PAGE  and immunoblotted for the presence of OGT. Membranes were then stripped and  blotted for PGC-1α.  C , gel filtration chromatography (SMART  system, Superdex 200 column, phosphate-buffered saline, 1% Nonidet P-40  buffer) of lysates from rat liver incubated on ice for 30 min with either  normal IgG or anti-OGT antibodies (AL28). Fractions were subjected to SDS-PAGE  and blotted using anti-PGC-1α, anti-OGT (DM-17), and anti CPB. Data are  representative of two experiments.
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    Image Search Results


    Substrate specificity of NAAA in the presenceof different stimulators.Recombinant NAAA (0.3–3 μg of protein) was allowed toreact with the indicated  14 C-labeled NAEs at 100 μMin the presence or absence of 0.1% Nonidet P-40,

    Journal: ACS Chemical Neuroscience

    Article Title: Endogenous Molecules Stimulating N-Acylethanolamine-Hydrolyzing Acid Amidase (NAAA)

    doi: 10.1021/cn300007s

    Figure Lengend Snippet: Substrate specificity of NAAA in the presenceof different stimulators.Recombinant NAAA (0.3–3 μg of protein) was allowed toreact with the indicated 14 C-labeled NAEs at 100 μMin the presence or absence of 0.1% Nonidet P-40,

    Article Snippet: [1-14 C]Palmitic acid was purchasedfrom PerkinElmer Life Science (Boston, MA); [1-14 C]stearicand [1-14 C]arachidonic acids from GE Healthcare (Piscataway,NJ); [1-14 C]oleic acid from Moravek Biochemicals (Brea,CA); nonradiolabeled NAEs from Cayman Chemical (Ann Arbor, MI); bovineserum albumin, 1,2-dioleoyl-PE, 1,2-dioleoyl-PC, 1,2-dipalmitoyl-PG,bovine liver PI, 1,2-dioleoyl-PS, bovine brain SM, 2-mercaptoethanol,dihydrolipoic and α-lipoic acids, and fetal calf serum fromSigma-Aldrich (St. Louis, MO); Triton X-100, Tween 20, DTT, l -cysteine hydrochloride, glutathione, formic and succinic acids,sodium (+)-tartrate, 3(2)- t -butyl-4-hydroxyanisole,ethanolamine, and Dulbecco’s modified Eagle’s mediumfrom Wako Pure Chemical (Osaka, Japan); Nonidet P-40, dl -homocysteine,potassium hydrogen phthalate, and l -(+)-tartaric and 3,3-dimethylglutaricacids from Nacalai Tesque (Kyoto, Japan); n -octyl-β- d -glucoside and CHAPS from Dojindo (Kumamoto, Japan); proteinassay dye reagent concentrate from Bio-Rad (Hercules, CA); precoatedsilica gel 60 F254 aluminum sheets for thin-layer chromatography(20 × 20 cm, 0.2-mm thickness) from Merck (Darmstadt, Germany);Lipofectamine 2000, TRIzol, and pcDNA3.1(+) from Life Technologies(Carlsbad, CA); PrimeScript RT reagent kit and SYBR Premix Ex Taq II from Takara Bio (Ohtsu, Japan); and HEK293 cells fromHealth Science Research Resources Bank (Osaka, Japan).

    Techniques: Recombinant, Labeling

    Effects of various buffers on NAAA activity. Recombinant NAAA (300ng of protein) was allowed to react with 100 μM [ 14 C]PEA in the presence of 3 mM DTT and 0.1% Nonidet P-40 at pH 4.5.The pH was adjusted with the following buffers (100 mM): citrate-Na

    Journal: ACS Chemical Neuroscience

    Article Title: Endogenous Molecules Stimulating N-Acylethanolamine-Hydrolyzing Acid Amidase (NAAA)

    doi: 10.1021/cn300007s

    Figure Lengend Snippet: Effects of various buffers on NAAA activity. Recombinant NAAA (300ng of protein) was allowed to react with 100 μM [ 14 C]PEA in the presence of 3 mM DTT and 0.1% Nonidet P-40 at pH 4.5.The pH was adjusted with the following buffers (100 mM): citrate-Na

    Article Snippet: [1-14 C]Palmitic acid was purchasedfrom PerkinElmer Life Science (Boston, MA); [1-14 C]stearicand [1-14 C]arachidonic acids from GE Healthcare (Piscataway,NJ); [1-14 C]oleic acid from Moravek Biochemicals (Brea,CA); nonradiolabeled NAEs from Cayman Chemical (Ann Arbor, MI); bovineserum albumin, 1,2-dioleoyl-PE, 1,2-dioleoyl-PC, 1,2-dipalmitoyl-PG,bovine liver PI, 1,2-dioleoyl-PS, bovine brain SM, 2-mercaptoethanol,dihydrolipoic and α-lipoic acids, and fetal calf serum fromSigma-Aldrich (St. Louis, MO); Triton X-100, Tween 20, DTT, l -cysteine hydrochloride, glutathione, formic and succinic acids,sodium (+)-tartrate, 3(2)- t -butyl-4-hydroxyanisole,ethanolamine, and Dulbecco’s modified Eagle’s mediumfrom Wako Pure Chemical (Osaka, Japan); Nonidet P-40, dl -homocysteine,potassium hydrogen phthalate, and l -(+)-tartaric and 3,3-dimethylglutaricacids from Nacalai Tesque (Kyoto, Japan); n -octyl-β- d -glucoside and CHAPS from Dojindo (Kumamoto, Japan); proteinassay dye reagent concentrate from Bio-Rad (Hercules, CA); precoatedsilica gel 60 F254 aluminum sheets for thin-layer chromatography(20 × 20 cm, 0.2-mm thickness) from Merck (Darmstadt, Germany);Lipofectamine 2000, TRIzol, and pcDNA3.1(+) from Life Technologies(Carlsbad, CA); PrimeScript RT reagent kit and SYBR Premix Ex Taq II from Takara Bio (Ohtsu, Japan); and HEK293 cells fromHealth Science Research Resources Bank (Osaka, Japan).

    Techniques: Activity Assay, Recombinant

    Effects of various phospholipid classes on NAAA activity.RecombinantNAAA (300 ng of protein) was allowed to react with 100 μM [ 14 C]PEA. (A) The reactions were carried out in the presenceof 3 mM DTT and either Nonidet P-40 at 0.1% (w/v) or the

    Journal: ACS Chemical Neuroscience

    Article Title: Endogenous Molecules Stimulating N-Acylethanolamine-Hydrolyzing Acid Amidase (NAAA)

    doi: 10.1021/cn300007s

    Figure Lengend Snippet: Effects of various phospholipid classes on NAAA activity.RecombinantNAAA (300 ng of protein) was allowed to react with 100 μM [ 14 C]PEA. (A) The reactions were carried out in the presenceof 3 mM DTT and either Nonidet P-40 at 0.1% (w/v) or the

    Article Snippet: [1-14 C]Palmitic acid was purchasedfrom PerkinElmer Life Science (Boston, MA); [1-14 C]stearicand [1-14 C]arachidonic acids from GE Healthcare (Piscataway,NJ); [1-14 C]oleic acid from Moravek Biochemicals (Brea,CA); nonradiolabeled NAEs from Cayman Chemical (Ann Arbor, MI); bovineserum albumin, 1,2-dioleoyl-PE, 1,2-dioleoyl-PC, 1,2-dipalmitoyl-PG,bovine liver PI, 1,2-dioleoyl-PS, bovine brain SM, 2-mercaptoethanol,dihydrolipoic and α-lipoic acids, and fetal calf serum fromSigma-Aldrich (St. Louis, MO); Triton X-100, Tween 20, DTT, l -cysteine hydrochloride, glutathione, formic and succinic acids,sodium (+)-tartrate, 3(2)- t -butyl-4-hydroxyanisole,ethanolamine, and Dulbecco’s modified Eagle’s mediumfrom Wako Pure Chemical (Osaka, Japan); Nonidet P-40, dl -homocysteine,potassium hydrogen phthalate, and l -(+)-tartaric and 3,3-dimethylglutaricacids from Nacalai Tesque (Kyoto, Japan); n -octyl-β- d -glucoside and CHAPS from Dojindo (Kumamoto, Japan); proteinassay dye reagent concentrate from Bio-Rad (Hercules, CA); precoatedsilica gel 60 F254 aluminum sheets for thin-layer chromatography(20 × 20 cm, 0.2-mm thickness) from Merck (Darmstadt, Germany);Lipofectamine 2000, TRIzol, and pcDNA3.1(+) from Life Technologies(Carlsbad, CA); PrimeScript RT reagent kit and SYBR Premix Ex Taq II from Takara Bio (Ohtsu, Japan); and HEK293 cells fromHealth Science Research Resources Bank (Osaka, Japan).

    Techniques: Activity Assay

    Effects of various thiol compounds onNAAA activity. RecombinantNAAA (300 ng of protein) was allowed to react with 100 μM [ 14 C]PEA. (A) The reactions were carried out in the presenceof 0.1% Nonidet P-40 and the indicated compound at 3 mM. (B)

    Journal: ACS Chemical Neuroscience

    Article Title: Endogenous Molecules Stimulating N-Acylethanolamine-Hydrolyzing Acid Amidase (NAAA)

    doi: 10.1021/cn300007s

    Figure Lengend Snippet: Effects of various thiol compounds onNAAA activity. RecombinantNAAA (300 ng of protein) was allowed to react with 100 μM [ 14 C]PEA. (A) The reactions were carried out in the presenceof 0.1% Nonidet P-40 and the indicated compound at 3 mM. (B)

    Article Snippet: [1-14 C]Palmitic acid was purchasedfrom PerkinElmer Life Science (Boston, MA); [1-14 C]stearicand [1-14 C]arachidonic acids from GE Healthcare (Piscataway,NJ); [1-14 C]oleic acid from Moravek Biochemicals (Brea,CA); nonradiolabeled NAEs from Cayman Chemical (Ann Arbor, MI); bovineserum albumin, 1,2-dioleoyl-PE, 1,2-dioleoyl-PC, 1,2-dipalmitoyl-PG,bovine liver PI, 1,2-dioleoyl-PS, bovine brain SM, 2-mercaptoethanol,dihydrolipoic and α-lipoic acids, and fetal calf serum fromSigma-Aldrich (St. Louis, MO); Triton X-100, Tween 20, DTT, l -cysteine hydrochloride, glutathione, formic and succinic acids,sodium (+)-tartrate, 3(2)- t -butyl-4-hydroxyanisole,ethanolamine, and Dulbecco’s modified Eagle’s mediumfrom Wako Pure Chemical (Osaka, Japan); Nonidet P-40, dl -homocysteine,potassium hydrogen phthalate, and l -(+)-tartaric and 3,3-dimethylglutaricacids from Nacalai Tesque (Kyoto, Japan); n -octyl-β- d -glucoside and CHAPS from Dojindo (Kumamoto, Japan); proteinassay dye reagent concentrate from Bio-Rad (Hercules, CA); precoatedsilica gel 60 F254 aluminum sheets for thin-layer chromatography(20 × 20 cm, 0.2-mm thickness) from Merck (Darmstadt, Germany);Lipofectamine 2000, TRIzol, and pcDNA3.1(+) from Life Technologies(Carlsbad, CA); PrimeScript RT reagent kit and SYBR Premix Ex Taq II from Takara Bio (Ohtsu, Japan); and HEK293 cells fromHealth Science Research Resources Bank (Osaka, Japan).

    Techniques: Activity Assay

    PGC-1α interacts with OGT. A , Fao cells were infected  with Ad-FLAG-PGC-1α and treated with insulin for 1 h prior to harvesting  and OGT co-immunoprecipitation ( IP ). Immunoprecipitates were  subjected to SDS-PAGE and immunoblotted ( IB ) for the presence of  PGC-1α. Membranes were then stripped and blotted for OGT. Immunoblots  are representative of three experiments.  B , Fao cells were infected  with Ad-FLAG-PGC-1α and treated with insulin for 1 h prior to harvesting  and FLAG co-immunoprecipitation. Immunoprecipitates were subjected to SDS-PAGE  and immunoblotted for the presence of OGT. Membranes were then stripped and  blotted for PGC-1α.  C , gel filtration chromatography (SMART  system, Superdex 200 column, phosphate-buffered saline, 1% Nonidet P-40  buffer) of lysates from rat liver incubated on ice for 30 min with either  normal IgG or anti-OGT antibodies (AL28). Fractions were subjected to SDS-PAGE  and blotted using anti-PGC-1α, anti-OGT (DM-17), and anti CPB. Data are  representative of two experiments.

    Journal: The Journal of Biological Chemistry

    Article Title: A PGC-1α-O-GlcNAc Transferase Complex Regulates FoxO Transcription Factor Activity in Response to Glucose

    doi: 10.1074/jbc.M808890200

    Figure Lengend Snippet: PGC-1α interacts with OGT. A , Fao cells were infected with Ad-FLAG-PGC-1α and treated with insulin for 1 h prior to harvesting and OGT co-immunoprecipitation ( IP ). Immunoprecipitates were subjected to SDS-PAGE and immunoblotted ( IB ) for the presence of PGC-1α. Membranes were then stripped and blotted for OGT. Immunoblots are representative of three experiments. B , Fao cells were infected with Ad-FLAG-PGC-1α and treated with insulin for 1 h prior to harvesting and FLAG co-immunoprecipitation. Immunoprecipitates were subjected to SDS-PAGE and immunoblotted for the presence of OGT. Membranes were then stripped and blotted for PGC-1α. C , gel filtration chromatography (SMART system, Superdex 200 column, phosphate-buffered saline, 1% Nonidet P-40 buffer) of lysates from rat liver incubated on ice for 30 min with either normal IgG or anti-OGT antibodies (AL28). Fractions were subjected to SDS-PAGE and blotted using anti-PGC-1α, anti-OGT (DM-17), and anti CPB. Data are representative of two experiments.

    Article Snippet: Rat liver was lysed in Tris-buffered saline with 1% Nonidet P-40 and incubated for 1 h on ice with either normal rabit IgG (Santa Cruz Biotechnology) or anti-Ogt (AL28) ( ).

    Techniques: Pyrolysis Gas Chromatography, Infection, Immunoprecipitation, SDS Page, Western Blot, Filtration, Chromatography, Incubation