non-esterified cholesterol Search Results


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  • 85
    Thermo Fisher gene exp soat2 mm00448823 m1
    Effects of Fabp1/Scp2/Scpx gene ablation and sex on hepatic cholesterol accumulation in high fat fed mice Male and female WT and TKO mice on a C57BL/6NCr background were pair-fed a HFD. Total C (A), free C (B) and cholesteryl ester (C) were measured as described in Methods. qRT-PCR was performed to determine the relative transcription of SREBP2 (Srebf2 gene ) (D) and ACAT2 ( <t>Soat2</t> gene) (G) as described in Methods. Western blots were performed to measure relative hepatic protein levels of HMGCS1 (E), HMGCR (F) and HSL (H) as described in Methods. The housekeeping gene COX4 was used as a loading control to normalize protein expression of HMGCS1 and HSL and GAPDH was used to normalize HMGCR. The inset in panels E, F and H show representative western blots of relative protein expression in each mouse group. Values represent the mean ± SEM, n=8. *p
    Gene Exp Soat2 Mm00448823 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Millipore water soluble non esterified cholesterol
    Effects of Fabp1/Scp2/Scpx gene ablation and sex on hepatic cholesterol accumulation in high fat fed mice Male and female WT and TKO mice on a C57BL/6NCr background were pair-fed a HFD. Total C (A), free C (B) and cholesteryl ester (C) were measured as described in Methods. qRT-PCR was performed to determine the relative transcription of SREBP2 (Srebf2 gene ) (D) and ACAT2 ( <t>Soat2</t> gene) (G) as described in Methods. Western blots were performed to measure relative hepatic protein levels of HMGCS1 (E), HMGCR (F) and HSL (H) as described in Methods. The housekeeping gene COX4 was used as a loading control to normalize protein expression of HMGCS1 and HSL and GAPDH was used to normalize HMGCR. The inset in panels E, F and H show representative western blots of relative protein expression in each mouse group. Values represent the mean ± SEM, n=8. *p
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    Millipore non esterified cholesterol
    Effects of Fabp1/Scp2/Scpx gene ablation and sex on hepatic cholesterol accumulation in high fat fed mice Male and female WT and TKO mice on a C57BL/6NCr background were pair-fed a HFD. Total C (A), free C (B) and cholesteryl ester (C) were measured as described in Methods. qRT-PCR was performed to determine the relative transcription of SREBP2 (Srebf2 gene ) (D) and ACAT2 ( <t>Soat2</t> gene) (G) as described in Methods. Western blots were performed to measure relative hepatic protein levels of HMGCS1 (E), HMGCR (F) and HSL (H) as described in Methods. The housekeeping gene COX4 was used as a loading control to normalize protein expression of HMGCS1 and HSL and GAPDH was used to normalize HMGCR. The inset in panels E, F and H show representative western blots of relative protein expression in each mouse group. Values represent the mean ± SEM, n=8. *p
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    FUJIFILM non esterified cholesterol
    Effects of Fabp1/Scp2/Scpx gene ablation and sex on hepatic cholesterol accumulation in high fat fed mice Male and female WT and TKO mice on a C57BL/6NCr background were pair-fed a HFD. Total C (A), free C (B) and cholesteryl ester (C) were measured as described in Methods. qRT-PCR was performed to determine the relative transcription of SREBP2 (Srebf2 gene ) (D) and ACAT2 ( <t>Soat2</t> gene) (G) as described in Methods. Western blots were performed to measure relative hepatic protein levels of HMGCS1 (E), HMGCR (F) and HSL (H) as described in Methods. The housekeeping gene COX4 was used as a loading control to normalize protein expression of HMGCS1 and HSL and GAPDH was used to normalize HMGCR. The inset in panels E, F and H show representative western blots of relative protein expression in each mouse group. Values represent the mean ± SEM, n=8. *p
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    DiaSys non esterified cholesterol
    Effects of Fabp1/Scp2/Scpx gene ablation and sex on hepatic cholesterol accumulation in high fat fed mice Male and female WT and TKO mice on a C57BL/6NCr background were pair-fed a HFD. Total C (A), free C (B) and cholesteryl ester (C) were measured as described in Methods. qRT-PCR was performed to determine the relative transcription of SREBP2 (Srebf2 gene ) (D) and ACAT2 ( <t>Soat2</t> gene) (G) as described in Methods. Western blots were performed to measure relative hepatic protein levels of HMGCS1 (E), HMGCR (F) and HSL (H) as described in Methods. The housekeeping gene COX4 was used as a loading control to normalize protein expression of HMGCS1 and HSL and GAPDH was used to normalize HMGCR. The inset in panels E, F and H show representative western blots of relative protein expression in each mouse group. Values represent the mean ± SEM, n=8. *p
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    Thermo Fisher non esterified fatty acids
    Effects of Fabp1/Scp2/Scpx gene ablation and sex on hepatic cholesterol accumulation in high fat fed mice Male and female WT and TKO mice on a C57BL/6NCr background were pair-fed a HFD. Total C (A), free C (B) and cholesteryl ester (C) were measured as described in Methods. qRT-PCR was performed to determine the relative transcription of SREBP2 (Srebf2 gene ) (D) and ACAT2 ( <t>Soat2</t> gene) (G) as described in Methods. Western blots were performed to measure relative hepatic protein levels of HMGCS1 (E), HMGCR (F) and HSL (H) as described in Methods. The housekeeping gene COX4 was used as a loading control to normalize protein expression of HMGCS1 and HSL and GAPDH was used to normalize HMGCR. The inset in panels E, F and H show representative western blots of relative protein expression in each mouse group. Values represent the mean ± SEM, n=8. *p
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    FUJIFILM non esterified fatty acids
    Effects of Fabp1/Scp2/Scpx gene ablation and sex on hepatic cholesterol accumulation in high fat fed mice Male and female WT and TKO mice on a C57BL/6NCr background were pair-fed a HFD. Total C (A), free C (B) and cholesteryl ester (C) were measured as described in Methods. qRT-PCR was performed to determine the relative transcription of SREBP2 (Srebf2 gene ) (D) and ACAT2 ( <t>Soat2</t> gene) (G) as described in Methods. Western blots were performed to measure relative hepatic protein levels of HMGCS1 (E), HMGCR (F) and HSL (H) as described in Methods. The housekeeping gene COX4 was used as a loading control to normalize protein expression of HMGCS1 and HSL and GAPDH was used to normalize HMGCR. The inset in panels E, F and H show representative western blots of relative protein expression in each mouse group. Values represent the mean ± SEM, n=8. *p
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    FUJIFILM non esterified fatty acid nefa
    Effects of Fabp1/Scp2/Scpx gene ablation and sex on hepatic cholesterol accumulation in high fat fed mice Male and female WT and TKO mice on a C57BL/6NCr background were pair-fed a HFD. Total C (A), free C (B) and cholesteryl ester (C) were measured as described in Methods. qRT-PCR was performed to determine the relative transcription of SREBP2 (Srebf2 gene ) (D) and ACAT2 ( <t>Soat2</t> gene) (G) as described in Methods. Western blots were performed to measure relative hepatic protein levels of HMGCS1 (E), HMGCR (F) and HSL (H) as described in Methods. The housekeeping gene COX4 was used as a loading control to normalize protein expression of HMGCS1 and HSL and GAPDH was used to normalize HMGCR. The inset in panels E, F and H show representative western blots of relative protein expression in each mouse group. Values represent the mean ± SEM, n=8. *p
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    Roche non esterified fatty acids
    Effects of Fabp1/Scp2/Scpx gene ablation and sex on hepatic cholesterol accumulation in high fat fed mice Male and female WT and TKO mice on a C57BL/6NCr background were pair-fed a HFD. Total C (A), free C (B) and cholesteryl ester (C) were measured as described in Methods. qRT-PCR was performed to determine the relative transcription of SREBP2 (Srebf2 gene ) (D) and ACAT2 ( <t>Soat2</t> gene) (G) as described in Methods. Western blots were performed to measure relative hepatic protein levels of HMGCS1 (E), HMGCR (F) and HSL (H) as described in Methods. The housekeeping gene COX4 was used as a loading control to normalize protein expression of HMGCS1 and HSL and GAPDH was used to normalize HMGCR. The inset in panels E, F and H show representative western blots of relative protein expression in each mouse group. Values represent the mean ± SEM, n=8. *p
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    FUJIFILM non esterified free fatty acid assay kits
    Effects of Fabp1/Scp2/Scpx gene ablation and sex on hepatic cholesterol accumulation in high fat fed mice Male and female WT and TKO mice on a C57BL/6NCr background were pair-fed a HFD. Total C (A), free C (B) and cholesteryl ester (C) were measured as described in Methods. qRT-PCR was performed to determine the relative transcription of SREBP2 (Srebf2 gene ) (D) and ACAT2 ( <t>Soat2</t> gene) (G) as described in Methods. Western blots were performed to measure relative hepatic protein levels of HMGCS1 (E), HMGCR (F) and HSL (H) as described in Methods. The housekeeping gene COX4 was used as a loading control to normalize protein expression of HMGCS1 and HSL and GAPDH was used to normalize HMGCR. The inset in panels E, F and H show representative western blots of relative protein expression in each mouse group. Values represent the mean ± SEM, n=8. *p
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    FUJIFILM non esterified free fatty acids
    Effects of Fabp1/Scp2/Scpx gene ablation and sex on hepatic cholesterol accumulation in high fat fed mice Male and female WT and TKO mice on a C57BL/6NCr background were pair-fed a HFD. Total C (A), free C (B) and cholesteryl ester (C) were measured as described in Methods. qRT-PCR was performed to determine the relative transcription of SREBP2 (Srebf2 gene ) (D) and ACAT2 ( <t>Soat2</t> gene) (G) as described in Methods. Western blots were performed to measure relative hepatic protein levels of HMGCS1 (E), HMGCR (F) and HSL (H) as described in Methods. The housekeeping gene COX4 was used as a loading control to normalize protein expression of HMGCS1 and HSL and GAPDH was used to normalize HMGCR. The inset in panels E, F and H show representative western blots of relative protein expression in each mouse group. Values represent the mean ± SEM, n=8. *p
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    Randox non esterified fatty acid nefa
    Effects of Fabp1/Scp2/Scpx gene ablation and sex on hepatic cholesterol accumulation in high fat fed mice Male and female WT and TKO mice on a C57BL/6NCr background were pair-fed a HFD. Total C (A), free C (B) and cholesteryl ester (C) were measured as described in Methods. qRT-PCR was performed to determine the relative transcription of SREBP2 (Srebf2 gene ) (D) and ACAT2 ( <t>Soat2</t> gene) (G) as described in Methods. Western blots were performed to measure relative hepatic protein levels of HMGCS1 (E), HMGCR (F) and HSL (H) as described in Methods. The housekeeping gene COX4 was used as a loading control to normalize protein expression of HMGCS1 and HSL and GAPDH was used to normalize HMGCR. The inset in panels E, F and H show representative western blots of relative protein expression in each mouse group. Values represent the mean ± SEM, n=8. *p
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    FUJIFILM non esterified fatty acid assay kits
    Effects of Fabp1/Scp2/Scpx gene ablation and sex on hepatic cholesterol accumulation in high fat fed mice Male and female WT and TKO mice on a C57BL/6NCr background were pair-fed a HFD. Total C (A), free C (B) and cholesteryl ester (C) were measured as described in Methods. qRT-PCR was performed to determine the relative transcription of SREBP2 (Srebf2 gene ) (D) and ACAT2 ( <t>Soat2</t> gene) (G) as described in Methods. Western blots were performed to measure relative hepatic protein levels of HMGCS1 (E), HMGCR (F) and HSL (H) as described in Methods. The housekeeping gene COX4 was used as a loading control to normalize protein expression of HMGCS1 and HSL and GAPDH was used to normalize HMGCR. The inset in panels E, F and H show representative western blots of relative protein expression in each mouse group. Values represent the mean ± SEM, n=8. *p
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    Effect of Zucker-fatty genotype and BCAA restriction on circulating amino acids and energy balance . Male Zucker-lean rats (ZLR) or Zucker-fatty rats (ZFR) were fed a custom low fat (LF) diet or an isonitrogenous isocaloric LF diet in which BCAA were restricted by 45% (LF-RES). (A) Circulating amino acid profiles. (B) Daily food intake (kcal/day). (C) Starting weight, final weight, and total weight gain (grams). (D) Liver and epididymal adipose tissue (eWAT) weights (grams). (E) Gastrocnemius, soleus and heart tissue weights (grams). n = 12–28 per group (F) Ambulation (total x and y beam breaks), (G) Heat (kcal/kg), (H) VO2 (mL/kg/hr), and (I) Respiratory exchange ratio (RER) measured hourly during a 24 h cycle following a 24 h acclimation period in the metabolic cages, during which the dark period was from 5PM to 5AM as shown by the gray bar, n = 9–12 per group. (J) Plasma triglycerides (TG; mg/dL), (K) glycerol (mg/dL), (L) non-esterified fatty acids <t>(NEFA;</t> μM/L), (M) <t>hydroxybutyrate</t> and ketones (mmol/L), and (N) lactate (μM/L), n = 9–15 per group. Data represent mean ± SEM. Statistical differences indicated by: *: P
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    Roche non esterified fatty acids nefa
    Effect of Zucker-fatty genotype and BCAA restriction on circulating amino acids and energy balance . Male Zucker-lean rats (ZLR) or Zucker-fatty rats (ZFR) were fed a custom low fat (LF) diet or an isonitrogenous isocaloric LF diet in which BCAA were restricted by 45% (LF-RES). (A) Circulating amino acid profiles. (B) Daily food intake (kcal/day). (C) Starting weight, final weight, and total weight gain (grams). (D) Liver and epididymal adipose tissue (eWAT) weights (grams). (E) Gastrocnemius, soleus and heart tissue weights (grams). n = 12–28 per group (F) Ambulation (total x and y beam breaks), (G) Heat (kcal/kg), (H) VO2 (mL/kg/hr), and (I) Respiratory exchange ratio (RER) measured hourly during a 24 h cycle following a 24 h acclimation period in the metabolic cages, during which the dark period was from 5PM to 5AM as shown by the gray bar, n = 9–12 per group. (J) Plasma triglycerides (TG; mg/dL), (K) glycerol (mg/dL), (L) non-esterified fatty acids <t>(NEFA;</t> μM/L), (M) <t>hydroxybutyrate</t> and ketones (mmol/L), and (N) lactate (μM/L), n = 9–15 per group. Data represent mean ± SEM. Statistical differences indicated by: *: P
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    ZenBio serum plasma fatty acid kit non esterified fatty acids detection 100 point kit
    Effect of Zucker-fatty genotype and BCAA restriction on circulating amino acids and energy balance . Male Zucker-lean rats (ZLR) or Zucker-fatty rats (ZFR) were fed a custom low fat (LF) diet or an isonitrogenous isocaloric LF diet in which BCAA were restricted by 45% (LF-RES). (A) Circulating amino acid profiles. (B) Daily food intake (kcal/day). (C) Starting weight, final weight, and total weight gain (grams). (D) Liver and epididymal adipose tissue (eWAT) weights (grams). (E) Gastrocnemius, soleus and heart tissue weights (grams). n = 12–28 per group (F) Ambulation (total x and y beam breaks), (G) Heat (kcal/kg), (H) VO2 (mL/kg/hr), and (I) Respiratory exchange ratio (RER) measured hourly during a 24 h cycle following a 24 h acclimation period in the metabolic cages, during which the dark period was from 5PM to 5AM as shown by the gray bar, n = 9–12 per group. (J) Plasma triglycerides (TG; mg/dL), (K) glycerol (mg/dL), (L) non-esterified fatty acids <t>(NEFA;</t> μM/L), (M) <t>hydroxybutyrate</t> and ketones (mmol/L), and (N) lactate (μM/L), n = 9–15 per group. Data represent mean ± SEM. Statistical differences indicated by: *: P
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    Roche nonesterified fatty acid kit
    The ROS generation of DVDMS ‐Mn‐ LP s under US irradiation. A, Fluorescence emission spectra of DVDMS ‐Mn‐ LP s in <t>DCFH</t> ‐ DA solution with different US irradiation time. B, The fluorescence intensity changes of DCFH ‐ DA at 521 nm with the increase of US irradiation time. C, Fluorescence emission spectra of DVDMS ‐Mn‐ LP s in DCFH ‐ DA solution with different US irradiation intensities. D, The fluorescence intensity changes of DCFH ‐ DA at 521 nm with the increase of US irradiation intensity. DCFH‐DA, <t>2′,7′‐dichlorofluorescin</t> diacetate; ROS, reactive oxygen species
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    The ROS generation of DVDMS ‐Mn‐ LP s under US irradiation. A, Fluorescence emission spectra of DVDMS ‐Mn‐ LP s in <t>DCFH</t> ‐ DA solution with different US irradiation time. B, The fluorescence intensity changes of DCFH ‐ DA at 521 nm with the increase of US irradiation time. C, Fluorescence emission spectra of DVDMS ‐Mn‐ LP s in DCFH ‐ DA solution with different US irradiation intensities. D, The fluorescence intensity changes of DCFH ‐ DA at 521 nm with the increase of US irradiation intensity. DCFH‐DA, <t>2′,7′‐dichlorofluorescin</t> diacetate; ROS, reactive oxygen species
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    FUJIFILM nonesterified fa
    The ROS generation of DVDMS ‐Mn‐ LP s under US irradiation. A, Fluorescence emission spectra of DVDMS ‐Mn‐ LP s in <t>DCFH</t> ‐ DA solution with different US irradiation time. B, The fluorescence intensity changes of DCFH ‐ DA at 521 nm with the increase of US irradiation time. C, Fluorescence emission spectra of DVDMS ‐Mn‐ LP s in DCFH ‐ DA solution with different US irradiation intensities. D, The fluorescence intensity changes of DCFH ‐ DA at 521 nm with the increase of US irradiation intensity. DCFH‐DA, <t>2′,7′‐dichlorofluorescin</t> diacetate; ROS, reactive oxygen species
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    Image Search Results


    Effects of Fabp1/Scp2/Scpx gene ablation and sex on hepatic cholesterol accumulation in high fat fed mice Male and female WT and TKO mice on a C57BL/6NCr background were pair-fed a HFD. Total C (A), free C (B) and cholesteryl ester (C) were measured as described in Methods. qRT-PCR was performed to determine the relative transcription of SREBP2 (Srebf2 gene ) (D) and ACAT2 ( Soat2 gene) (G) as described in Methods. Western blots were performed to measure relative hepatic protein levels of HMGCS1 (E), HMGCR (F) and HSL (H) as described in Methods. The housekeeping gene COX4 was used as a loading control to normalize protein expression of HMGCS1 and HSL and GAPDH was used to normalize HMGCR. The inset in panels E, F and H show representative western blots of relative protein expression in each mouse group. Values represent the mean ± SEM, n=8. *p

    Journal: Biochimica et biophysica acta

    Article Title: Ablating both Fabp1 and Scp2/Scpx (TKO) Induces Hepatic Phospholipid and Cholesterol Accumulation in High Fat-fed Mice

    doi: 10.1016/j.bbalip.2017.12.013

    Figure Lengend Snippet: Effects of Fabp1/Scp2/Scpx gene ablation and sex on hepatic cholesterol accumulation in high fat fed mice Male and female WT and TKO mice on a C57BL/6NCr background were pair-fed a HFD. Total C (A), free C (B) and cholesteryl ester (C) were measured as described in Methods. qRT-PCR was performed to determine the relative transcription of SREBP2 (Srebf2 gene ) (D) and ACAT2 ( Soat2 gene) (G) as described in Methods. Western blots were performed to measure relative hepatic protein levels of HMGCS1 (E), HMGCR (F) and HSL (H) as described in Methods. The housekeeping gene COX4 was used as a loading control to normalize protein expression of HMGCS1 and HSL and GAPDH was used to normalize HMGCR. The inset in panels E, F and H show representative western blots of relative protein expression in each mouse group. Values represent the mean ± SEM, n=8. *p

    Article Snippet: The following probes were obtained from Applied Biosystems (Foster City, CA) for quantitating gene specific mRNA levels in mouse liver homogenates as follows: glycerol-3-phosphate acyltransferase (GPAT) ( Gpam ; Mm00833328_m1), 1-acylglycerol-3-phosphate-O-acyltransferase (AGPAT) ( Agpat2 ; Mm00458880_m1), Lipin 2 ( Lpin2 ; Mm00522390_m1), diacylglycerol acyltransferase (DGAT) ( Dgat2 ; Mm00499536_m1), acetyl-coA carboxylase (ACC1) ( Acaca ; Mm01304285_m1) , fatty acid synthase (FASN) ( Fasn ; Mm00662319_m1), organic anion-transporting polypeptide 1 (OATP1) ( Slco1 ; Mm01267414_m1), organic anion-transporting polypeptide 2 (OATP2) ( Slc22a7 ; Mm00460672_m1), carnitine palmitoyltransferase 1a (CPT1A) ( Cpt1a ; Mm00550438_m1), carnitine palmitoyltransferase 2 (CPT2) ( Cpt2 ; Mm00487202_m1), acyl-CoA oxidase-1 (ACOX1) ( Acox1 ; Mm00443579_m1), acetyl-CoA acetyltransferase (ACAT2) ( Soat2 ; Mm00448823_m1), bile salt export pump (BSEP) ( Abcb11 ; Mm00445168_m1), sterol regulatory element-binding protein 2 (SREBP2) ( Srebf2 ; Mm01306289_m1) and cytochrome P7A1, CYP7A1 ( Cyp7a1 , Mm00484152_m1).

    Techniques: Mouse Assay, Quantitative RT-PCR, Western Blot, Expressing

    Effect of Zucker-fatty genotype and BCAA restriction on circulating amino acids and energy balance . Male Zucker-lean rats (ZLR) or Zucker-fatty rats (ZFR) were fed a custom low fat (LF) diet or an isonitrogenous isocaloric LF diet in which BCAA were restricted by 45% (LF-RES). (A) Circulating amino acid profiles. (B) Daily food intake (kcal/day). (C) Starting weight, final weight, and total weight gain (grams). (D) Liver and epididymal adipose tissue (eWAT) weights (grams). (E) Gastrocnemius, soleus and heart tissue weights (grams). n = 12–28 per group (F) Ambulation (total x and y beam breaks), (G) Heat (kcal/kg), (H) VO2 (mL/kg/hr), and (I) Respiratory exchange ratio (RER) measured hourly during a 24 h cycle following a 24 h acclimation period in the metabolic cages, during which the dark period was from 5PM to 5AM as shown by the gray bar, n = 9–12 per group. (J) Plasma triglycerides (TG; mg/dL), (K) glycerol (mg/dL), (L) non-esterified fatty acids (NEFA; μM/L), (M) hydroxybutyrate and ketones (mmol/L), and (N) lactate (μM/L), n = 9–15 per group. Data represent mean ± SEM. Statistical differences indicated by: *: P

    Journal: Molecular Metabolism

    Article Title: Branched-chain amino acid restriction in Zucker-fatty rats improves muscle insulin sensitivity by enhancing efficiency of fatty acid oxidation and acyl-glycine export

    doi: 10.1016/j.molmet.2016.04.006

    Figure Lengend Snippet: Effect of Zucker-fatty genotype and BCAA restriction on circulating amino acids and energy balance . Male Zucker-lean rats (ZLR) or Zucker-fatty rats (ZFR) were fed a custom low fat (LF) diet or an isonitrogenous isocaloric LF diet in which BCAA were restricted by 45% (LF-RES). (A) Circulating amino acid profiles. (B) Daily food intake (kcal/day). (C) Starting weight, final weight, and total weight gain (grams). (D) Liver and epididymal adipose tissue (eWAT) weights (grams). (E) Gastrocnemius, soleus and heart tissue weights (grams). n = 12–28 per group (F) Ambulation (total x and y beam breaks), (G) Heat (kcal/kg), (H) VO2 (mL/kg/hr), and (I) Respiratory exchange ratio (RER) measured hourly during a 24 h cycle following a 24 h acclimation period in the metabolic cages, during which the dark period was from 5PM to 5AM as shown by the gray bar, n = 9–12 per group. (J) Plasma triglycerides (TG; mg/dL), (K) glycerol (mg/dL), (L) non-esterified fatty acids (NEFA; μM/L), (M) hydroxybutyrate and ketones (mmol/L), and (N) lactate (μM/L), n = 9–15 per group. Data represent mean ± SEM. Statistical differences indicated by: *: P

    Article Snippet: Other plasma analytes were measured on a Beckman DxC600 autoanalyzer, using reagents for lactate, total cholesterol, and triglycerides from Beckman, and non-esterified fatty acids (NEFA) and ketones (total and 3-hydroxybutyrate) from Wako (Richmond, VA).

    Techniques:

    The ROS generation of DVDMS ‐Mn‐ LP s under US irradiation. A, Fluorescence emission spectra of DVDMS ‐Mn‐ LP s in DCFH ‐ DA solution with different US irradiation time. B, The fluorescence intensity changes of DCFH ‐ DA at 521 nm with the increase of US irradiation time. C, Fluorescence emission spectra of DVDMS ‐Mn‐ LP s in DCFH ‐ DA solution with different US irradiation intensities. D, The fluorescence intensity changes of DCFH ‐ DA at 521 nm with the increase of US irradiation intensity. DCFH‐DA, 2′,7′‐dichlorofluorescin diacetate; ROS, reactive oxygen species

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Theranostic nanosensitizers for highly efficient MR/fluorescence imaging‐guided sonodynamic therapy of gliomas, et al. Theranostic nanosensitizers for highly efficient MR/fluorescence imaging‐guided sonodynamic therapy of gliomas

    doi: 10.1111/jcmm.13811

    Figure Lengend Snippet: The ROS generation of DVDMS ‐Mn‐ LP s under US irradiation. A, Fluorescence emission spectra of DVDMS ‐Mn‐ LP s in DCFH ‐ DA solution with different US irradiation time. B, The fluorescence intensity changes of DCFH ‐ DA at 521 nm with the increase of US irradiation time. C, Fluorescence emission spectra of DVDMS ‐Mn‐ LP s in DCFH ‐ DA solution with different US irradiation intensities. D, The fluorescence intensity changes of DCFH ‐ DA at 521 nm with the increase of US irradiation intensity. DCFH‐DA, 2′,7′‐dichlorofluorescin diacetate; ROS, reactive oxygen species

    Article Snippet: Cholesterol, 2′,7′‐dichlorofluorescin diacetate (DCFH‐DA) and 4′,6‐diamidino‐2‐phenylindole (DAPI) were purchased from Sigma‐Aldrich (St. Louis, MO, USA).

    Techniques: Irradiation, Fluorescence