non-essential amino-acid Search Results


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  • 95
    Millipore mem non essential amino acids
    Initial provision of serum, bFGF or SCF is not necessary for conversion of mouse PGCs into EG cells in presence of 2i-LIF. ( A ) Schematic of derivation protocol to test requirement for <t>FCS</t> and bFGF. Primordial germ cells (PGCs) are plated either directly into 2i-LIF only or into FCS-LIF plus bFGF. ( B ) Quantitation of EG cell colony formation following derivation directly into 2i-LIF or transfer to 2i-LIF after the first <t>medium</t> change. ( C ) Schematic of derivation protocol to test requirement for stem cell factor (SCF). PGCs were plated directly into 2i-LIF either onto Sl 4 -m220 feeders, Sl/Sl 4 feeders or Sl/Sl 4 feeders with the addition of soluble SCF. ( D ) Quantitation of EG cell colony formation following derivation directly into 2i-LIF in the presence of membrane-bound SCF, no SCF or soluble SCF.
    Mem Non Essential Amino Acids, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 536 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mem non essential amino acids - by Bioz Stars, 2020-02
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    90
    Thermo Fisher mem non essential amino acids solution
    TA-LF complexes promotes delivery of encapsulated therapeutic drugs to LC cells. MTS assay of drug-encapsulated TA-LF complexes against ( a ) A540 and ( b ) <t>H1299</t> LC cells. Cells (5 × 103) were seeded in a 96-well plate and left overnight for cell attachment to the plate , the cells were treated with indicated concentrations of gemcitabine, carboplatin, and irinotecan and their respective drug-encapsulated TA-LF complexes for 48 h. Cell viability was determined using MTS assay. TA at the concentrations used to make TA-LF complexes were used as controls. The data were presented in the form of line graphs as percent viable cells compared to untreated cells in <t>medium.</t> Data presented as mean ± SEM (each treatment, n = 6). Cytotoxicity of TA-LF-drug formulations were significant compared to free drugs (* p
    Mem Non Essential Amino Acids Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher non essential amino acid
    TA-LF complexes promotes delivery of encapsulated therapeutic drugs to LC cells. MTS assay of drug-encapsulated TA-LF complexes against ( a ) A540 and ( b ) <t>H1299</t> LC cells. Cells (5 × 103) were seeded in a 96-well plate and left overnight for cell attachment to the plate , the cells were treated with indicated concentrations of gemcitabine, carboplatin, and irinotecan and their respective drug-encapsulated TA-LF complexes for 48 h. Cell viability was determined using MTS assay. TA at the concentrations used to make TA-LF complexes were used as controls. The data were presented in the form of line graphs as percent viable cells compared to untreated cells in <t>medium.</t> Data presented as mean ± SEM (each treatment, n = 6). Cytotoxicity of TA-LF-drug formulations were significant compared to free drugs (* p
    Non Essential Amino Acid, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1028 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    EuroClone non essential amino acid
    TA-LF complexes promotes delivery of encapsulated therapeutic drugs to LC cells. MTS assay of drug-encapsulated TA-LF complexes against ( a ) A540 and ( b ) <t>H1299</t> LC cells. Cells (5 × 103) were seeded in a 96-well plate and left overnight for cell attachment to the plate , the cells were treated with indicated concentrations of gemcitabine, carboplatin, and irinotecan and their respective drug-encapsulated TA-LF complexes for 48 h. Cell viability was determined using MTS assay. TA at the concentrations used to make TA-LF complexes were used as controls. The data were presented in the form of line graphs as percent viable cells compared to untreated cells in <t>medium.</t> Data presented as mean ± SEM (each treatment, n = 6). Cytotoxicity of TA-LF-drug formulations were significant compared to free drugs (* p
    Non Essential Amino Acid, supplied by EuroClone, used in various techniques. Bioz Stars score: 93/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Cellgro non essential amino acid
    TA-LF complexes promotes delivery of encapsulated therapeutic drugs to LC cells. MTS assay of drug-encapsulated TA-LF complexes against ( a ) A540 and ( b ) <t>H1299</t> LC cells. Cells (5 × 103) were seeded in a 96-well plate and left overnight for cell attachment to the plate , the cells were treated with indicated concentrations of gemcitabine, carboplatin, and irinotecan and their respective drug-encapsulated TA-LF complexes for 48 h. Cell viability was determined using MTS assay. TA at the concentrations used to make TA-LF complexes were used as controls. The data were presented in the form of line graphs as percent viable cells compared to untreated cells in <t>medium.</t> Data presented as mean ± SEM (each treatment, n = 6). Cytotoxicity of TA-LF-drug formulations were significant compared to free drugs (* p
    Non Essential Amino Acid, supplied by Cellgro, used in various techniques. Bioz Stars score: 89/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Biochrom non essential amino acid
    TA-LF complexes promotes delivery of encapsulated therapeutic drugs to LC cells. MTS assay of drug-encapsulated TA-LF complexes against ( a ) A540 and ( b ) <t>H1299</t> LC cells. Cells (5 × 103) were seeded in a 96-well plate and left overnight for cell attachment to the plate , the cells were treated with indicated concentrations of gemcitabine, carboplatin, and irinotecan and their respective drug-encapsulated TA-LF complexes for 48 h. Cell viability was determined using MTS assay. TA at the concentrations used to make TA-LF complexes were used as controls. The data were presented in the form of line graphs as percent viable cells compared to untreated cells in <t>medium.</t> Data presented as mean ± SEM (each treatment, n = 6). Cytotoxicity of TA-LF-drug formulations were significant compared to free drugs (* p
    Non Essential Amino Acid, supplied by Biochrom, used in various techniques. Bioz Stars score: 89/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    GE Healthcare non essential amino acid
    TA-LF complexes promotes delivery of encapsulated therapeutic drugs to LC cells. MTS assay of drug-encapsulated TA-LF complexes against ( a ) A540 and ( b ) <t>H1299</t> LC cells. Cells (5 × 103) were seeded in a 96-well plate and left overnight for cell attachment to the plate , the cells were treated with indicated concentrations of gemcitabine, carboplatin, and irinotecan and their respective drug-encapsulated TA-LF complexes for 48 h. Cell viability was determined using MTS assay. TA at the concentrations used to make TA-LF complexes were used as controls. The data were presented in the form of line graphs as percent viable cells compared to untreated cells in <t>medium.</t> Data presented as mean ± SEM (each treatment, n = 6). Cytotoxicity of TA-LF-drug formulations were significant compared to free drugs (* p
    Non Essential Amino Acid, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Cambrex non essential amino acid
    TA-LF complexes promotes delivery of encapsulated therapeutic drugs to LC cells. MTS assay of drug-encapsulated TA-LF complexes against ( a ) A540 and ( b ) <t>H1299</t> LC cells. Cells (5 × 103) were seeded in a 96-well plate and left overnight for cell attachment to the plate , the cells were treated with indicated concentrations of gemcitabine, carboplatin, and irinotecan and their respective drug-encapsulated TA-LF complexes for 48 h. Cell viability was determined using MTS assay. TA at the concentrations used to make TA-LF complexes were used as controls. The data were presented in the form of line graphs as percent viable cells compared to untreated cells in <t>medium.</t> Data presented as mean ± SEM (each treatment, n = 6). Cytotoxicity of TA-LF-drug formulations were significant compared to free drugs (* p
    Non Essential Amino Acid, supplied by Cambrex, used in various techniques. Bioz Stars score: 89/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Mediatech non essential amino acid
    TA-LF complexes promotes delivery of encapsulated therapeutic drugs to LC cells. MTS assay of drug-encapsulated TA-LF complexes against ( a ) A540 and ( b ) <t>H1299</t> LC cells. Cells (5 × 103) were seeded in a 96-well plate and left overnight for cell attachment to the plate , the cells were treated with indicated concentrations of gemcitabine, carboplatin, and irinotecan and their respective drug-encapsulated TA-LF complexes for 48 h. Cell viability was determined using MTS assay. TA at the concentrations used to make TA-LF complexes were used as controls. The data were presented in the form of line graphs as percent viable cells compared to untreated cells in <t>medium.</t> Data presented as mean ± SEM (each treatment, n = 6). Cytotoxicity of TA-LF-drug formulations were significant compared to free drugs (* p
    Non Essential Amino Acid, supplied by Mediatech, used in various techniques. Bioz Stars score: 90/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Merck KGaA non essential amino acid
    TA-LF complexes promotes delivery of encapsulated therapeutic drugs to LC cells. MTS assay of drug-encapsulated TA-LF complexes against ( a ) A540 and ( b ) <t>H1299</t> LC cells. Cells (5 × 103) were seeded in a 96-well plate and left overnight for cell attachment to the plate , the cells were treated with indicated concentrations of gemcitabine, carboplatin, and irinotecan and their respective drug-encapsulated TA-LF complexes for 48 h. Cell viability was determined using MTS assay. TA at the concentrations used to make TA-LF complexes were used as controls. The data were presented in the form of line graphs as percent viable cells compared to untreated cells in <t>medium.</t> Data presented as mean ± SEM (each treatment, n = 6). Cytotoxicity of TA-LF-drug formulations were significant compared to free drugs (* p
    Non Essential Amino Acid, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 89/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Biological Industries Inc non essential amino acid
    TA-LF complexes promotes delivery of encapsulated therapeutic drugs to LC cells. MTS assay of drug-encapsulated TA-LF complexes against ( a ) A540 and ( b ) <t>H1299</t> LC cells. Cells (5 × 103) were seeded in a 96-well plate and left overnight for cell attachment to the plate , the cells were treated with indicated concentrations of gemcitabine, carboplatin, and irinotecan and their respective drug-encapsulated TA-LF complexes for 48 h. Cell viability was determined using MTS assay. TA at the concentrations used to make TA-LF complexes were used as controls. The data were presented in the form of line graphs as percent viable cells compared to untreated cells in <t>medium.</t> Data presented as mean ± SEM (each treatment, n = 6). Cytotoxicity of TA-LF-drug formulations were significant compared to free drugs (* p
    Non Essential Amino Acid, supplied by Biological Industries Inc, used in various techniques. Bioz Stars score: 89/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    FUJIFILM non essential amino acid
    TA-LF complexes promotes delivery of encapsulated therapeutic drugs to LC cells. MTS assay of drug-encapsulated TA-LF complexes against ( a ) A540 and ( b ) <t>H1299</t> LC cells. Cells (5 × 103) were seeded in a 96-well plate and left overnight for cell attachment to the plate , the cells were treated with indicated concentrations of gemcitabine, carboplatin, and irinotecan and their respective drug-encapsulated TA-LF complexes for 48 h. Cell viability was determined using MTS assay. TA at the concentrations used to make TA-LF complexes were used as controls. The data were presented in the form of line graphs as percent viable cells compared to untreated cells in <t>medium.</t> Data presented as mean ± SEM (each treatment, n = 6). Cytotoxicity of TA-LF-drug formulations were significant compared to free drugs (* p
    Non Essential Amino Acid, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 89/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Nacalai non essential amino acid
    TA-LF complexes promotes delivery of encapsulated therapeutic drugs to LC cells. MTS assay of drug-encapsulated TA-LF complexes against ( a ) A540 and ( b ) <t>H1299</t> LC cells. Cells (5 × 103) were seeded in a 96-well plate and left overnight for cell attachment to the plate , the cells were treated with indicated concentrations of gemcitabine, carboplatin, and irinotecan and their respective drug-encapsulated TA-LF complexes for 48 h. Cell viability was determined using MTS assay. TA at the concentrations used to make TA-LF complexes were used as controls. The data were presented in the form of line graphs as percent viable cells compared to untreated cells in <t>medium.</t> Data presented as mean ± SEM (each treatment, n = 6). Cytotoxicity of TA-LF-drug formulations were significant compared to free drugs (* p
    Non Essential Amino Acid, supplied by Nacalai, used in various techniques. Bioz Stars score: 89/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Mediatech non essential amino acid supplement
    TA-LF complexes promotes delivery of encapsulated therapeutic drugs to LC cells. MTS assay of drug-encapsulated TA-LF complexes against ( a ) A540 and ( b ) <t>H1299</t> LC cells. Cells (5 × 103) were seeded in a 96-well plate and left overnight for cell attachment to the plate , the cells were treated with indicated concentrations of gemcitabine, carboplatin, and irinotecan and their respective drug-encapsulated TA-LF complexes for 48 h. Cell viability was determined using MTS assay. TA at the concentrations used to make TA-LF complexes were used as controls. The data were presented in the form of line graphs as percent viable cells compared to untreated cells in <t>medium.</t> Data presented as mean ± SEM (each treatment, n = 6). Cytotoxicity of TA-LF-drug formulations were significant compared to free drugs (* p
    Non Essential Amino Acid Supplement, supplied by Mediatech, used in various techniques. Bioz Stars score: 78/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Avantor non essential amino acids
    TA-LF complexes promotes delivery of encapsulated therapeutic drugs to LC cells. MTS assay of drug-encapsulated TA-LF complexes against ( a ) A540 and ( b ) <t>H1299</t> LC cells. Cells (5 × 103) were seeded in a 96-well plate and left overnight for cell attachment to the plate , the cells were treated with indicated concentrations of gemcitabine, carboplatin, and irinotecan and their respective drug-encapsulated TA-LF complexes for 48 h. Cell viability was determined using MTS assay. TA at the concentrations used to make TA-LF complexes were used as controls. The data were presented in the form of line graphs as percent viable cells compared to untreated cells in <t>medium.</t> Data presented as mean ± SEM (each treatment, n = 6). Cytotoxicity of TA-LF-drug formulations were significant compared to free drugs (* p
    Non Essential Amino Acids, supplied by Avantor, used in various techniques. Bioz Stars score: 97/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    PAA Laboratories non essential amino acids
    TA-LF complexes promotes delivery of encapsulated therapeutic drugs to LC cells. MTS assay of drug-encapsulated TA-LF complexes against ( a ) A540 and ( b ) <t>H1299</t> LC cells. Cells (5 × 103) were seeded in a 96-well plate and left overnight for cell attachment to the plate , the cells were treated with indicated concentrations of gemcitabine, carboplatin, and irinotecan and their respective drug-encapsulated TA-LF complexes for 48 h. Cell viability was determined using MTS assay. TA at the concentrations used to make TA-LF complexes were used as controls. The data were presented in the form of line graphs as percent viable cells compared to untreated cells in <t>medium.</t> Data presented as mean ± SEM (each treatment, n = 6). Cytotoxicity of TA-LF-drug formulations were significant compared to free drugs (* p
    Non Essential Amino Acids, supplied by PAA Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 186 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Wisent Corporation non essential amino acids
    TA-LF complexes promotes delivery of encapsulated therapeutic drugs to LC cells. MTS assay of drug-encapsulated TA-LF complexes against ( a ) A540 and ( b ) <t>H1299</t> LC cells. Cells (5 × 103) were seeded in a 96-well plate and left overnight for cell attachment to the plate , the cells were treated with indicated concentrations of gemcitabine, carboplatin, and irinotecan and their respective drug-encapsulated TA-LF complexes for 48 h. Cell viability was determined using MTS assay. TA at the concentrations used to make TA-LF complexes were used as controls. The data were presented in the form of line graphs as percent viable cells compared to untreated cells in <t>medium.</t> Data presented as mean ± SEM (each treatment, n = 6). Cytotoxicity of TA-LF-drug formulations were significant compared to free drugs (* p
    Non Essential Amino Acids, supplied by Wisent Corporation, used in various techniques. Bioz Stars score: 96/100, based on 87 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Thermo Fisher 1x non essential amino acid
    TA-LF complexes promotes delivery of encapsulated therapeutic drugs to LC cells. MTS assay of drug-encapsulated TA-LF complexes against ( a ) A540 and ( b ) <t>H1299</t> LC cells. Cells (5 × 103) were seeded in a 96-well plate and left overnight for cell attachment to the plate , the cells were treated with indicated concentrations of gemcitabine, carboplatin, and irinotecan and their respective drug-encapsulated TA-LF complexes for 48 h. Cell viability was determined using MTS assay. TA at the concentrations used to make TA-LF complexes were used as controls. The data were presented in the form of line graphs as percent viable cells compared to untreated cells in <t>medium.</t> Data presented as mean ± SEM (each treatment, n = 6). Cytotoxicity of TA-LF-drug formulations were significant compared to free drugs (* p
    1x Non Essential Amino Acid, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 131 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Irvine Scientific non essential amino acids
    TA-LF complexes promotes delivery of encapsulated therapeutic drugs to LC cells. MTS assay of drug-encapsulated TA-LF complexes against ( a ) A540 and ( b ) <t>H1299</t> LC cells. Cells (5 × 103) were seeded in a 96-well plate and left overnight for cell attachment to the plate , the cells were treated with indicated concentrations of gemcitabine, carboplatin, and irinotecan and their respective drug-encapsulated TA-LF complexes for 48 h. Cell viability was determined using MTS assay. TA at the concentrations used to make TA-LF complexes were used as controls. The data were presented in the form of line graphs as percent viable cells compared to untreated cells in <t>medium.</t> Data presented as mean ± SEM (each treatment, n = 6). Cytotoxicity of TA-LF-drug formulations were significant compared to free drugs (* p
    Non Essential Amino Acids, supplied by Irvine Scientific, used in various techniques. Bioz Stars score: 94/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Roche non essential amino acids
    TA-LF complexes promotes delivery of encapsulated therapeutic drugs to LC cells. MTS assay of drug-encapsulated TA-LF complexes against ( a ) A540 and ( b ) <t>H1299</t> LC cells. Cells (5 × 103) were seeded in a 96-well plate and left overnight for cell attachment to the plate , the cells were treated with indicated concentrations of gemcitabine, carboplatin, and irinotecan and their respective drug-encapsulated TA-LF complexes for 48 h. Cell viability was determined using MTS assay. TA at the concentrations used to make TA-LF complexes were used as controls. The data were presented in the form of line graphs as percent viable cells compared to untreated cells in <t>medium.</t> Data presented as mean ± SEM (each treatment, n = 6). Cytotoxicity of TA-LF-drug formulations were significant compared to free drugs (* p
    Non Essential Amino Acids, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Fisher Scientific non essential amino acids
    TA-LF complexes promotes delivery of encapsulated therapeutic drugs to LC cells. MTS assay of drug-encapsulated TA-LF complexes against ( a ) A540 and ( b ) <t>H1299</t> LC cells. Cells (5 × 103) were seeded in a 96-well plate and left overnight for cell attachment to the plate , the cells were treated with indicated concentrations of gemcitabine, carboplatin, and irinotecan and their respective drug-encapsulated TA-LF complexes for 48 h. Cell viability was determined using MTS assay. TA at the concentrations used to make TA-LF complexes were used as controls. The data were presented in the form of line graphs as percent viable cells compared to untreated cells in <t>medium.</t> Data presented as mean ± SEM (each treatment, n = 6). Cytotoxicity of TA-LF-drug formulations were significant compared to free drugs (* p
    Non Essential Amino Acids, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 97/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    82
    GE Healthcare non essential amino acid neaa
    TA-LF complexes promotes delivery of encapsulated therapeutic drugs to LC cells. MTS assay of drug-encapsulated TA-LF complexes against ( a ) A540 and ( b ) <t>H1299</t> LC cells. Cells (5 × 103) were seeded in a 96-well plate and left overnight for cell attachment to the plate , the cells were treated with indicated concentrations of gemcitabine, carboplatin, and irinotecan and their respective drug-encapsulated TA-LF complexes for 48 h. Cell viability was determined using MTS assay. TA at the concentrations used to make TA-LF complexes were used as controls. The data were presented in the form of line graphs as percent viable cells compared to untreated cells in <t>medium.</t> Data presented as mean ± SEM (each treatment, n = 6). Cytotoxicity of TA-LF-drug formulations were significant compared to free drugs (* p
    Non Essential Amino Acid Neaa, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 82/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Flow Laboratories non essential amino acids
    TA-LF complexes promotes delivery of encapsulated therapeutic drugs to LC cells. MTS assay of drug-encapsulated TA-LF complexes against ( a ) A540 and ( b ) <t>H1299</t> LC cells. Cells (5 × 103) were seeded in a 96-well plate and left overnight for cell attachment to the plate , the cells were treated with indicated concentrations of gemcitabine, carboplatin, and irinotecan and their respective drug-encapsulated TA-LF complexes for 48 h. Cell viability was determined using MTS assay. TA at the concentrations used to make TA-LF complexes were used as controls. The data were presented in the form of line graphs as percent viable cells compared to untreated cells in <t>medium.</t> Data presented as mean ± SEM (each treatment, n = 6). Cytotoxicity of TA-LF-drug formulations were significant compared to free drugs (* p
    Non Essential Amino Acids, supplied by Flow Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Biowest SAS non essential amino acids
    TA-LF complexes promotes delivery of encapsulated therapeutic drugs to LC cells. MTS assay of drug-encapsulated TA-LF complexes against ( a ) A540 and ( b ) <t>H1299</t> LC cells. Cells (5 × 103) were seeded in a 96-well plate and left overnight for cell attachment to the plate , the cells were treated with indicated concentrations of gemcitabine, carboplatin, and irinotecan and their respective drug-encapsulated TA-LF complexes for 48 h. Cell viability was determined using MTS assay. TA at the concentrations used to make TA-LF complexes were used as controls. The data were presented in the form of line graphs as percent viable cells compared to untreated cells in <t>medium.</t> Data presented as mean ± SEM (each treatment, n = 6). Cytotoxicity of TA-LF-drug formulations were significant compared to free drugs (* p
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    TA-LF complexes promotes delivery of encapsulated therapeutic drugs to LC cells. MTS assay of drug-encapsulated TA-LF complexes against ( a ) A540 and ( b ) <t>H1299</t> LC cells. Cells (5 × 103) were seeded in a 96-well plate and left overnight for cell attachment to the plate , the cells were treated with indicated concentrations of gemcitabine, carboplatin, and irinotecan and their respective drug-encapsulated TA-LF complexes for 48 h. Cell viability was determined using MTS assay. TA at the concentrations used to make TA-LF complexes were used as controls. The data were presented in the form of line graphs as percent viable cells compared to untreated cells in <t>medium.</t> Data presented as mean ± SEM (each treatment, n = 6). Cytotoxicity of TA-LF-drug formulations were significant compared to free drugs (* p
    Non Essential Amino Acids, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 97/100, based on 239 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TA-LF complexes promotes delivery of encapsulated therapeutic drugs to LC cells. MTS assay of drug-encapsulated TA-LF complexes against ( a ) A540 and ( b ) <t>H1299</t> LC cells. Cells (5 × 103) were seeded in a 96-well plate and left overnight for cell attachment to the plate , the cells were treated with indicated concentrations of gemcitabine, carboplatin, and irinotecan and their respective drug-encapsulated TA-LF complexes for 48 h. Cell viability was determined using MTS assay. TA at the concentrations used to make TA-LF complexes were used as controls. The data were presented in the form of line graphs as percent viable cells compared to untreated cells in <t>medium.</t> Data presented as mean ± SEM (each treatment, n = 6). Cytotoxicity of TA-LF-drug formulations were significant compared to free drugs (* p
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    PAN - Biotech non essential amino acids
    TA-LF complexes promotes delivery of encapsulated therapeutic drugs to LC cells. MTS assay of drug-encapsulated TA-LF complexes against ( a ) A540 and ( b ) <t>H1299</t> LC cells. Cells (5 × 103) were seeded in a 96-well plate and left overnight for cell attachment to the plate , the cells were treated with indicated concentrations of gemcitabine, carboplatin, and irinotecan and their respective drug-encapsulated TA-LF complexes for 48 h. Cell viability was determined using MTS assay. TA at the concentrations used to make TA-LF complexes were used as controls. The data were presented in the form of line graphs as percent viable cells compared to untreated cells in <t>medium.</t> Data presented as mean ± SEM (each treatment, n = 6). Cytotoxicity of TA-LF-drug formulations were significant compared to free drugs (* p
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    TA-LF complexes promotes delivery of encapsulated therapeutic drugs to LC cells. MTS assay of drug-encapsulated TA-LF complexes against ( a ) A540 and ( b ) <t>H1299</t> LC cells. Cells (5 × 103) were seeded in a 96-well plate and left overnight for cell attachment to the plate , the cells were treated with indicated concentrations of gemcitabine, carboplatin, and irinotecan and their respective drug-encapsulated TA-LF complexes for 48 h. Cell viability was determined using MTS assay. TA at the concentrations used to make TA-LF complexes were used as controls. The data were presented in the form of line graphs as percent viable cells compared to untreated cells in <t>medium.</t> Data presented as mean ± SEM (each treatment, n = 6). Cytotoxicity of TA-LF-drug formulations were significant compared to free drugs (* p
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    TA-LF complexes promotes delivery of encapsulated therapeutic drugs to LC cells. MTS assay of drug-encapsulated TA-LF complexes against ( a ) A540 and ( b ) <t>H1299</t> LC cells. Cells (5 × 103) were seeded in a 96-well plate and left overnight for cell attachment to the plate , the cells were treated with indicated concentrations of gemcitabine, carboplatin, and irinotecan and their respective drug-encapsulated TA-LF complexes for 48 h. Cell viability was determined using MTS assay. TA at the concentrations used to make TA-LF complexes were used as controls. The data were presented in the form of line graphs as percent viable cells compared to untreated cells in <t>medium.</t> Data presented as mean ± SEM (each treatment, n = 6). Cytotoxicity of TA-LF-drug formulations were significant compared to free drugs (* p
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    TA-LF complexes promotes delivery of encapsulated therapeutic drugs to LC cells. MTS assay of drug-encapsulated TA-LF complexes against ( a ) A540 and ( b ) <t>H1299</t> LC cells. Cells (5 × 103) were seeded in a 96-well plate and left overnight for cell attachment to the plate , the cells were treated with indicated concentrations of gemcitabine, carboplatin, and irinotecan and their respective drug-encapsulated TA-LF complexes for 48 h. Cell viability was determined using MTS assay. TA at the concentrations used to make TA-LF complexes were used as controls. The data were presented in the form of line graphs as percent viable cells compared to untreated cells in <t>medium.</t> Data presented as mean ± SEM (each treatment, n = 6). Cytotoxicity of TA-LF-drug formulations were significant compared to free drugs (* p
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    Thermo Fisher 100×non essential amino acid
    TA-LF complexes promotes delivery of encapsulated therapeutic drugs to LC cells. MTS assay of drug-encapsulated TA-LF complexes against ( a ) A540 and ( b ) <t>H1299</t> LC cells. Cells (5 × 103) were seeded in a 96-well plate and left overnight for cell attachment to the plate , the cells were treated with indicated concentrations of gemcitabine, carboplatin, and irinotecan and their respective drug-encapsulated TA-LF complexes for 48 h. Cell viability was determined using MTS assay. TA at the concentrations used to make TA-LF complexes were used as controls. The data were presented in the form of line graphs as percent viable cells compared to untreated cells in <t>medium.</t> Data presented as mean ± SEM (each treatment, n = 6). Cytotoxicity of TA-LF-drug formulations were significant compared to free drugs (* p
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    Image Search Results


    Initial provision of serum, bFGF or SCF is not necessary for conversion of mouse PGCs into EG cells in presence of 2i-LIF. ( A ) Schematic of derivation protocol to test requirement for FCS and bFGF. Primordial germ cells (PGCs) are plated either directly into 2i-LIF only or into FCS-LIF plus bFGF. ( B ) Quantitation of EG cell colony formation following derivation directly into 2i-LIF or transfer to 2i-LIF after the first medium change. ( C ) Schematic of derivation protocol to test requirement for stem cell factor (SCF). PGCs were plated directly into 2i-LIF either onto Sl 4 -m220 feeders, Sl/Sl 4 feeders or Sl/Sl 4 feeders with the addition of soluble SCF. ( D ) Quantitation of EG cell colony formation following derivation directly into 2i-LIF in the presence of membrane-bound SCF, no SCF or soluble SCF.

    Journal: Development (Cambridge, England)

    Article Title: Embryonic germ cells from mice and rats exhibit properties consistent with a generic pluripotent ground state

    doi: 10.1242/dev.050427

    Figure Lengend Snippet: Initial provision of serum, bFGF or SCF is not necessary for conversion of mouse PGCs into EG cells in presence of 2i-LIF. ( A ) Schematic of derivation protocol to test requirement for FCS and bFGF. Primordial germ cells (PGCs) are plated either directly into 2i-LIF only or into FCS-LIF plus bFGF. ( B ) Quantitation of EG cell colony formation following derivation directly into 2i-LIF or transfer to 2i-LIF after the first medium change. ( C ) Schematic of derivation protocol to test requirement for stem cell factor (SCF). PGCs were plated directly into 2i-LIF either onto Sl 4 -m220 feeders, Sl/Sl 4 feeders or Sl/Sl 4 feeders with the addition of soluble SCF. ( D ) Quantitation of EG cell colony formation following derivation directly into 2i-LIF in the presence of membrane-bound SCF, no SCF or soluble SCF.

    Article Snippet: FCS-LIF conditions comprise DMEM-F12 medium supplemented with 15% FCS, 0.1% MEM non-essential amino acids, 4 mM glutamate, 2 mM sodium pyruvate, 0.1 mM 2-mecaptanethanol and recombinant mouse LIF (1200 U/ml; ESGRO, Chemicon) or human recombinant LIF generated in-house by transient transfection of Cos7 cells with plasmid encoding human LIF.

    Techniques: Quantitation Assay

    Mouse EG cells can be maintained and derived in 2i-LIF. ( A ) Phase contrast and fluorescence images of Oct4-ΔPE-GFP EG cells. ( B ) Phase contrast and fluorescence images of Oct4-ΔPE-GFP EG cells cultured in: FCS-LIF on a MEF feeder layer; FCS-LIF after one passage without feeders; 2i-LIF without feeders. ( C ) Flow cytometry analysis of Oct4-ΔPE-GFP EG cells cultured in 2i-LIF compared with FCS-LIF on feeders. Gates were set to eliminate feeders and cell debris. ( D ) Colony-forming assay on Oct4-ΔPE-GFP EG cells. Colonies were scored for alkaline phosphatase (AP) and designated positive, negative or mixed. Data are means of two biological replicates. ( E ) Schematic of EG cell derivation protocol. On day 3, medium was changed to either FCS-LIF or 2i-LIF. ( F ) Comparison of GFP-positive EG cell colony formation following addition of 2i-LIF on day 3 compared with FCS-LIF. ( G ) High-contribution coat colour chimaeras generated with agouti EG cells derived in 2i-LIF injected into C57BL/6 blastocysts. ( H ) Chimaera, C57BL/6 mate, and mixed litter of agouti and black pups. Scale bars: 100 μm in A; 25 μm in B.

    Journal: Development (Cambridge, England)

    Article Title: Embryonic germ cells from mice and rats exhibit properties consistent with a generic pluripotent ground state

    doi: 10.1242/dev.050427

    Figure Lengend Snippet: Mouse EG cells can be maintained and derived in 2i-LIF. ( A ) Phase contrast and fluorescence images of Oct4-ΔPE-GFP EG cells. ( B ) Phase contrast and fluorescence images of Oct4-ΔPE-GFP EG cells cultured in: FCS-LIF on a MEF feeder layer; FCS-LIF after one passage without feeders; 2i-LIF without feeders. ( C ) Flow cytometry analysis of Oct4-ΔPE-GFP EG cells cultured in 2i-LIF compared with FCS-LIF on feeders. Gates were set to eliminate feeders and cell debris. ( D ) Colony-forming assay on Oct4-ΔPE-GFP EG cells. Colonies were scored for alkaline phosphatase (AP) and designated positive, negative or mixed. Data are means of two biological replicates. ( E ) Schematic of EG cell derivation protocol. On day 3, medium was changed to either FCS-LIF or 2i-LIF. ( F ) Comparison of GFP-positive EG cell colony formation following addition of 2i-LIF on day 3 compared with FCS-LIF. ( G ) High-contribution coat colour chimaeras generated with agouti EG cells derived in 2i-LIF injected into C57BL/6 blastocysts. ( H ) Chimaera, C57BL/6 mate, and mixed litter of agouti and black pups. Scale bars: 100 μm in A; 25 μm in B.

    Article Snippet: FCS-LIF conditions comprise DMEM-F12 medium supplemented with 15% FCS, 0.1% MEM non-essential amino acids, 4 mM glutamate, 2 mM sodium pyruvate, 0.1 mM 2-mecaptanethanol and recombinant mouse LIF (1200 U/ml; ESGRO, Chemicon) or human recombinant LIF generated in-house by transient transfection of Cos7 cells with plasmid encoding human LIF.

    Techniques: Derivative Assay, Fluorescence, Cell Culture, Flow Cytometry, Cytometry, Generated, Injection

    TA-LF complexes promotes delivery of encapsulated therapeutic drugs to LC cells. MTS assay of drug-encapsulated TA-LF complexes against ( a ) A540 and ( b ) H1299 LC cells. Cells (5 × 103) were seeded in a 96-well plate and left overnight for cell attachment to the plate , the cells were treated with indicated concentrations of gemcitabine, carboplatin, and irinotecan and their respective drug-encapsulated TA-LF complexes for 48 h. Cell viability was determined using MTS assay. TA at the concentrations used to make TA-LF complexes were used as controls. The data were presented in the form of line graphs as percent viable cells compared to untreated cells in medium. Data presented as mean ± SEM (each treatment, n = 6). Cytotoxicity of TA-LF-drug formulations were significant compared to free drugs (* p

    Journal: Pharmaceutics

    Article Title: Tannic Acid-Lung Fluid Assemblies Promote Interaction and Delivery of Drugs to Lung Cancer Cells

    doi: 10.3390/pharmaceutics10030111

    Figure Lengend Snippet: TA-LF complexes promotes delivery of encapsulated therapeutic drugs to LC cells. MTS assay of drug-encapsulated TA-LF complexes against ( a ) A540 and ( b ) H1299 LC cells. Cells (5 × 103) were seeded in a 96-well plate and left overnight for cell attachment to the plate , the cells were treated with indicated concentrations of gemcitabine, carboplatin, and irinotecan and their respective drug-encapsulated TA-LF complexes for 48 h. Cell viability was determined using MTS assay. TA at the concentrations used to make TA-LF complexes were used as controls. The data were presented in the form of line graphs as percent viable cells compared to untreated cells in medium. Data presented as mean ± SEM (each treatment, n = 6). Cytotoxicity of TA-LF-drug formulations were significant compared to free drugs (* p

    Article Snippet: The cancer cell lines cultured under sterile condition in Dulbecco’s Modified Eagle’s medium (DMEM for A549) and Roswell Park Memorial Institute medium (RPMI for H1299) supplemented with 4.5 g/L of glucose, 10 nM of nonessential amino acids (# 11140076, Gibco, Thermo Fisher Scientific, Grand Island, NY, USA), 100 mM of sodium pyruvate (#11360070, Gibco), 1× antibiotic/antimycotic (#15240062, Gibco), and 10% heat-inactivated FBS (#10438026 Thermo Fisher).

    Techniques: MTS Assay, Cell Attachment Assay