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    Bio-Rad non denaturing page
    Development and characterization of aptamer-nanomedicine (A) Self-assembly scheme of aptamer nanostructure carrying gene specific and cell-specific entities, and the formed <t>Apt-NS/DOX-siRNA</t> (Apt-NMed) inducing targeted chemotherapy and gene therapy. ( B ) Formation of Apt-NS/siRNA was confirmed by change in the size of ssDNA oligonucleotide mixture (ssDNA #1, ssDNA #2, ssDNA #3) on 5% <t>PAGE</t> gel; Lane 1, markers between 50 and 1000 bp; Lane 2, DNA oligonucleotide #1; Lane 3, DNA oligonucleotide #2; Lane 4, DNA oligonucleotide #3; Lane 5, mixture of #1+#2; Lane 6, mixture of #1+#2+#3. ( C ) Formed Apt-NS/siRNA produced two fragments after restriction enzyme digestion with HindIII and BamHI , indicating successful formation of Apt-NS/siRNA; Lane marked with M shows markers between 50 and 1000 bp. ( D ) Saturation point determination of DOX loading in Apt-NS/siRNA by mixing different Apt-NS/siRNA to DOX ratios. Changes in fluorescence were used to monitor DOX intercalation into Apt-NS/siRNA; red arrow indicates saturation point of DOX loading in Apt-NS/siRNA. Size (E) and zeta-potential (F) of Apt-NS/siRNA and Apt-NMed were examined by Zeta-sizer nano-detector.
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    Development and characterization of aptamer-nanomedicine (A) Self-assembly scheme of aptamer nanostructure carrying gene specific and cell-specific entities, and the formed <t>Apt-NS/DOX-siRNA</t> (Apt-NMed) inducing targeted chemotherapy and gene therapy. ( B ) Formation of Apt-NS/siRNA was confirmed by change in the size of ssDNA oligonucleotide mixture (ssDNA #1, ssDNA #2, ssDNA #3) on 5% <t>PAGE</t> gel; Lane 1, markers between 50 and 1000 bp; Lane 2, DNA oligonucleotide #1; Lane 3, DNA oligonucleotide #2; Lane 4, DNA oligonucleotide #3; Lane 5, mixture of #1+#2; Lane 6, mixture of #1+#2+#3. ( C ) Formed Apt-NS/siRNA produced two fragments after restriction enzyme digestion with HindIII and BamHI , indicating successful formation of Apt-NS/siRNA; Lane marked with M shows markers between 50 and 1000 bp. ( D ) Saturation point determination of DOX loading in Apt-NS/siRNA by mixing different Apt-NS/siRNA to DOX ratios. Changes in fluorescence were used to monitor DOX intercalation into Apt-NS/siRNA; red arrow indicates saturation point of DOX loading in Apt-NS/siRNA. Size (E) and zeta-potential (F) of Apt-NS/siRNA and Apt-NMed were examined by Zeta-sizer nano-detector.
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    Development and characterization of aptamer-nanomedicine (A) Self-assembly scheme of aptamer nanostructure carrying gene specific and cell-specific entities, and the formed <t>Apt-NS/DOX-siRNA</t> (Apt-NMed) inducing targeted chemotherapy and gene therapy. ( B ) Formation of Apt-NS/siRNA was confirmed by change in the size of ssDNA oligonucleotide mixture (ssDNA #1, ssDNA #2, ssDNA #3) on 5% <t>PAGE</t> gel; Lane 1, markers between 50 and 1000 bp; Lane 2, DNA oligonucleotide #1; Lane 3, DNA oligonucleotide #2; Lane 4, DNA oligonucleotide #3; Lane 5, mixture of #1+#2; Lane 6, mixture of #1+#2+#3. ( C ) Formed Apt-NS/siRNA produced two fragments after restriction enzyme digestion with HindIII and BamHI , indicating successful formation of Apt-NS/siRNA; Lane marked with M shows markers between 50 and 1000 bp. ( D ) Saturation point determination of DOX loading in Apt-NS/siRNA by mixing different Apt-NS/siRNA to DOX ratios. Changes in fluorescence were used to monitor DOX intercalation into Apt-NS/siRNA; red arrow indicates saturation point of DOX loading in Apt-NS/siRNA. Size (E) and zeta-potential (F) of Apt-NS/siRNA and Apt-NMed were examined by Zeta-sizer nano-detector.
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    Development and characterization of aptamer-nanomedicine (A) Self-assembly scheme of aptamer nanostructure carrying gene specific and cell-specific entities, and the formed <t>Apt-NS/DOX-siRNA</t> (Apt-NMed) inducing targeted chemotherapy and gene therapy. ( B ) Formation of Apt-NS/siRNA was confirmed by change in the size of ssDNA oligonucleotide mixture (ssDNA #1, ssDNA #2, ssDNA #3) on 5% <t>PAGE</t> gel; Lane 1, markers between 50 and 1000 bp; Lane 2, DNA oligonucleotide #1; Lane 3, DNA oligonucleotide #2; Lane 4, DNA oligonucleotide #3; Lane 5, mixture of #1+#2; Lane 6, mixture of #1+#2+#3. ( C ) Formed Apt-NS/siRNA produced two fragments after restriction enzyme digestion with HindIII and BamHI , indicating successful formation of Apt-NS/siRNA; Lane marked with M shows markers between 50 and 1000 bp. ( D ) Saturation point determination of DOX loading in Apt-NS/siRNA by mixing different Apt-NS/siRNA to DOX ratios. Changes in fluorescence were used to monitor DOX intercalation into Apt-NS/siRNA; red arrow indicates saturation point of DOX loading in Apt-NS/siRNA. Size (E) and zeta-potential (F) of Apt-NS/siRNA and Apt-NMed were examined by Zeta-sizer nano-detector.
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    Development and characterization of aptamer-nanomedicine (A) Self-assembly scheme of aptamer nanostructure carrying gene specific and cell-specific entities, and the formed Apt-NS/DOX-siRNA (Apt-NMed) inducing targeted chemotherapy and gene therapy. ( B ) Formation of Apt-NS/siRNA was confirmed by change in the size of ssDNA oligonucleotide mixture (ssDNA #1, ssDNA #2, ssDNA #3) on 5% PAGE gel; Lane 1, markers between 50 and 1000 bp; Lane 2, DNA oligonucleotide #1; Lane 3, DNA oligonucleotide #2; Lane 4, DNA oligonucleotide #3; Lane 5, mixture of #1+#2; Lane 6, mixture of #1+#2+#3. ( C ) Formed Apt-NS/siRNA produced two fragments after restriction enzyme digestion with HindIII and BamHI , indicating successful formation of Apt-NS/siRNA; Lane marked with M shows markers between 50 and 1000 bp. ( D ) Saturation point determination of DOX loading in Apt-NS/siRNA by mixing different Apt-NS/siRNA to DOX ratios. Changes in fluorescence were used to monitor DOX intercalation into Apt-NS/siRNA; red arrow indicates saturation point of DOX loading in Apt-NS/siRNA. Size (E) and zeta-potential (F) of Apt-NS/siRNA and Apt-NMed were examined by Zeta-sizer nano-detector.

    Journal: Small (Weinheim an der Bergstrasse, Germany)

    Article Title: Self-Assembled Aptamer-Nanomedicine for Targeted Chemotherapy and Gene Therapy

    doi: 10.1002/smll.201702103

    Figure Lengend Snippet: Development and characterization of aptamer-nanomedicine (A) Self-assembly scheme of aptamer nanostructure carrying gene specific and cell-specific entities, and the formed Apt-NS/DOX-siRNA (Apt-NMed) inducing targeted chemotherapy and gene therapy. ( B ) Formation of Apt-NS/siRNA was confirmed by change in the size of ssDNA oligonucleotide mixture (ssDNA #1, ssDNA #2, ssDNA #3) on 5% PAGE gel; Lane 1, markers between 50 and 1000 bp; Lane 2, DNA oligonucleotide #1; Lane 3, DNA oligonucleotide #2; Lane 4, DNA oligonucleotide #3; Lane 5, mixture of #1+#2; Lane 6, mixture of #1+#2+#3. ( C ) Formed Apt-NS/siRNA produced two fragments after restriction enzyme digestion with HindIII and BamHI , indicating successful formation of Apt-NS/siRNA; Lane marked with M shows markers between 50 and 1000 bp. ( D ) Saturation point determination of DOX loading in Apt-NS/siRNA by mixing different Apt-NS/siRNA to DOX ratios. Changes in fluorescence were used to monitor DOX intercalation into Apt-NS/siRNA; red arrow indicates saturation point of DOX loading in Apt-NS/siRNA. Size (E) and zeta-potential (F) of Apt-NS/siRNA and Apt-NMed were examined by Zeta-sizer nano-detector.

    Article Snippet: First, the formed Apt-NS/siRNA (1μg) was separated on 5% non-denaturing PAGE, and analyzed on a Gel Doc EZ imager (Bio-Rad, Hercules, CA).

    Techniques: Polyacrylamide Gel Electrophoresis, Produced, Fluorescence