Journal: Small (Weinheim an der Bergstrasse, Germany)
Article Title: Self-Assembled Aptamer-Nanomedicine for Targeted Chemotherapy and Gene Therapy
Figure Lengend Snippet: Development and characterization of aptamer-nanomedicine (A) Self-assembly scheme of aptamer nanostructure carrying gene specific and cell-specific entities, and the formed Apt-NS/DOX-siRNA (Apt-NMed) inducing targeted chemotherapy and gene therapy. ( B ) Formation of Apt-NS/siRNA was confirmed by change in the size of ssDNA oligonucleotide mixture (ssDNA #1, ssDNA #2, ssDNA #3) on 5% PAGE gel; Lane 1, markers between 50 and 1000 bp; Lane 2, DNA oligonucleotide #1; Lane 3, DNA oligonucleotide #2; Lane 4, DNA oligonucleotide #3; Lane 5, mixture of #1+#2; Lane 6, mixture of #1+#2+#3. ( C ) Formed Apt-NS/siRNA produced two fragments after restriction enzyme digestion with HindIII and BamHI , indicating successful formation of Apt-NS/siRNA; Lane marked with M shows markers between 50 and 1000 bp. ( D ) Saturation point determination of DOX loading in Apt-NS/siRNA by mixing different Apt-NS/siRNA to DOX ratios. Changes in fluorescence were used to monitor DOX intercalation into Apt-NS/siRNA; red arrow indicates saturation point of DOX loading in Apt-NS/siRNA. Size (E) and zeta-potential (F) of Apt-NS/siRNA and Apt-NMed were examined by Zeta-sizer nano-detector.
Article Snippet: First, the formed Apt-NS/siRNA (1μg) was separated on 5% non-denaturing PAGE, and analyzed on a Gel Doc EZ imager (Bio-Rad, Hercules, CA).
Techniques: Polyacrylamide Gel Electrophoresis, Produced, Fluorescence