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  • 94
    New England Biolabs nla iv
    Sequence characteristics of transforming DNA. A. Variable DUS positions and restriction profiles in transforming DNA plasmids. Transforming DNA plasmids are based on pilG (black) interrupted by an erythromycin resistance insert, ermC , (gray). DUS ( 5′- ATGCCGTCTGAA-3′ ) or reverse complimentary DUS ( 5′-TTCAGACGGCAT-3′ ) are inserted in three different positions A, B and C as marked above the bar. All numbers refer to the nucleotide position following the start codon (1) of pilG. The 137 nucleotide long Bam HI-fragment which is removed in pDV-b, pDV-c versions is shown in white with black stripes. B. <t>Nla</t> IV restriction profile of <t>rpoB</t> and location of selective SNP. The homologous 723 nt long PCR fragment of an internal part of the meningococcal rpoB gene used in transformation contains two Nla IV restriction sites on both sides of the selective SNP responsible for rifampicin resistance in the recipient.
    Nla Iv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nla iv/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nla iv - by Bioz Stars, 2022-08
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    94
    New England Biolabs nlaiv
    Sequence characteristics of transforming DNA. A. Variable DUS positions and restriction profiles in transforming DNA plasmids. Transforming DNA plasmids are based on pilG (black) interrupted by an erythromycin resistance insert, ermC , (gray). DUS ( 5′- ATGCCGTCTGAA-3′ ) or reverse complimentary DUS ( 5′-TTCAGACGGCAT-3′ ) are inserted in three different positions A, B and C as marked above the bar. All numbers refer to the nucleotide position following the start codon (1) of pilG. The 137 nucleotide long Bam HI-fragment which is removed in pDV-b, pDV-c versions is shown in white with black stripes. B. <t>Nla</t> IV restriction profile of <t>rpoB</t> and location of selective SNP. The homologous 723 nt long PCR fragment of an internal part of the meningococcal rpoB gene used in transformation contains two Nla IV restriction sites on both sides of the selective SNP responsible for rifampicin resistance in the recipient.
    Nlaiv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nlaiv/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nlaiv - by Bioz Stars, 2022-08
    94/100 stars
      Buy from Supplier

    88
    Thermo Fisher bspl i
    Agarose gel electrophoresis of the semi-nested PCR amplification of a 432-bp of the <t>gdh</t> gene of G. duodenalis . Lanes 1, negative control (no DNA); 2, positive control; 3-5, patient's samples; M, DNA ladder (A). Digestion of PCR product targeting the gdh gene using <t>BspL</t> I ( Nla IV) to distinguish AI (90-, 120-, 150-bp) and AII (70-, 80-, 90-, 120-bp) genotypes. Lane 1, undigested PCR product; 2 and 3, AI genotype; 4 and 5, AII genotype; 6, mixed infections (AI+AII); M, DNA ladder (B). Agarose gel electrophoresis of the semi-nested PCR amplification of a 476-bp of the tpi gene of the G. duodenalis assemblage A. Lanes 1, positive control; 2-6, patient's samples; 7, negative control (no DNA); M, DNA ladder (C). Digestion of PCR product targeting the tpi gene using Rsa I to distinguish AI (437-, 15-bp) and AII (232-, 235-, 15-bp) genotypes. Lane 1, undigested PCR product of the G. duodenalis assemblage A; 2-4; AI genotype; 5, AII genotype; 6 and 7, mixed infections (AI+AII); M, DNA ladder (D). Sizes in base pairs are denoted by the numbers at left.
    Bspl I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bspl i/product/Thermo Fisher
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bspl i - by Bioz Stars, 2022-08
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    Image Search Results


    Sequence characteristics of transforming DNA. A. Variable DUS positions and restriction profiles in transforming DNA plasmids. Transforming DNA plasmids are based on pilG (black) interrupted by an erythromycin resistance insert, ermC , (gray). DUS ( 5′- ATGCCGTCTGAA-3′ ) or reverse complimentary DUS ( 5′-TTCAGACGGCAT-3′ ) are inserted in three different positions A, B and C as marked above the bar. All numbers refer to the nucleotide position following the start codon (1) of pilG. The 137 nucleotide long Bam HI-fragment which is removed in pDV-b, pDV-c versions is shown in white with black stripes. B. Nla IV restriction profile of rpoB and location of selective SNP. The homologous 723 nt long PCR fragment of an internal part of the meningococcal rpoB gene used in transformation contains two Nla IV restriction sites on both sides of the selective SNP responsible for rifampicin resistance in the recipient.

    Journal: PLoS ONE

    Article Title: Restriction and Sequence Alterations Affect DNA Uptake Sequence-Dependent Transformation in Neisseria meningitidis

    doi: 10.1371/journal.pone.0039742

    Figure Lengend Snippet: Sequence characteristics of transforming DNA. A. Variable DUS positions and restriction profiles in transforming DNA plasmids. Transforming DNA plasmids are based on pilG (black) interrupted by an erythromycin resistance insert, ermC , (gray). DUS ( 5′- ATGCCGTCTGAA-3′ ) or reverse complimentary DUS ( 5′-TTCAGACGGCAT-3′ ) are inserted in three different positions A, B and C as marked above the bar. All numbers refer to the nucleotide position following the start codon (1) of pilG. The 137 nucleotide long Bam HI-fragment which is removed in pDV-b, pDV-c versions is shown in white with black stripes. B. Nla IV restriction profile of rpoB and location of selective SNP. The homologous 723 nt long PCR fragment of an internal part of the meningococcal rpoB gene used in transformation contains two Nla IV restriction sites on both sides of the selective SNP responsible for rifampicin resistance in the recipient.

    Article Snippet: Intact and Nla IV (New England Biolabs) restricted rpoB PCR fragments were tested for their transforming abilities.

    Techniques: Sequencing, Polymerase Chain Reaction, Transformation Assay

    Nla IV restriction does not affect homologous DNA transformation in N. meningitidis MC58 wildtype or in the Nla IV null mutant. The Y axis shows the number of rifampicin-resistant CFU/total CFU 10 9 on a log scale. Along the X-axis are shown the two different DNA substrates (5 ng/ml), rpoB PCR fragment and rpoB PCR fragment pre-digested with Nla IV. For further details on the transforming DNA please consult Figure 1 and Table 2 . The transformation frequencies in the wildtype and in the Nla IV null mutant backgrounds are not statistically significant from each other.

    Journal: PLoS ONE

    Article Title: Restriction and Sequence Alterations Affect DNA Uptake Sequence-Dependent Transformation in Neisseria meningitidis

    doi: 10.1371/journal.pone.0039742

    Figure Lengend Snippet: Nla IV restriction does not affect homologous DNA transformation in N. meningitidis MC58 wildtype or in the Nla IV null mutant. The Y axis shows the number of rifampicin-resistant CFU/total CFU 10 9 on a log scale. Along the X-axis are shown the two different DNA substrates (5 ng/ml), rpoB PCR fragment and rpoB PCR fragment pre-digested with Nla IV. For further details on the transforming DNA please consult Figure 1 and Table 2 . The transformation frequencies in the wildtype and in the Nla IV null mutant backgrounds are not statistically significant from each other.

    Article Snippet: Intact and Nla IV (New England Biolabs) restricted rpoB PCR fragments were tested for their transforming abilities.

    Techniques: Transformation Assay, Mutagenesis, Polymerase Chain Reaction

    Agarose gel electrophoresis of the semi-nested PCR amplification of a 432-bp of the gdh gene of G. duodenalis . Lanes 1, negative control (no DNA); 2, positive control; 3-5, patient's samples; M, DNA ladder (A). Digestion of PCR product targeting the gdh gene using BspL I ( Nla IV) to distinguish AI (90-, 120-, 150-bp) and AII (70-, 80-, 90-, 120-bp) genotypes. Lane 1, undigested PCR product; 2 and 3, AI genotype; 4 and 5, AII genotype; 6, mixed infections (AI+AII); M, DNA ladder (B). Agarose gel electrophoresis of the semi-nested PCR amplification of a 476-bp of the tpi gene of the G. duodenalis assemblage A. Lanes 1, positive control; 2-6, patient's samples; 7, negative control (no DNA); M, DNA ladder (C). Digestion of PCR product targeting the tpi gene using Rsa I to distinguish AI (437-, 15-bp) and AII (232-, 235-, 15-bp) genotypes. Lane 1, undigested PCR product of the G. duodenalis assemblage A; 2-4; AI genotype; 5, AII genotype; 6 and 7, mixed infections (AI+AII); M, DNA ladder (D). Sizes in base pairs are denoted by the numbers at left.

    Journal: Microbes and infection

    Article Title: Adaptive immune response in symptomatic and asymptomatic enteric protozoal infection: evidence for a determining role of parasite genetic heterogeneity in host immunity to human giardiasis

    doi: 10.1016/j.micinf.2016.06.009

    Figure Lengend Snippet: Agarose gel electrophoresis of the semi-nested PCR amplification of a 432-bp of the gdh gene of G. duodenalis . Lanes 1, negative control (no DNA); 2, positive control; 3-5, patient's samples; M, DNA ladder (A). Digestion of PCR product targeting the gdh gene using BspL I ( Nla IV) to distinguish AI (90-, 120-, 150-bp) and AII (70-, 80-, 90-, 120-bp) genotypes. Lane 1, undigested PCR product; 2 and 3, AI genotype; 4 and 5, AII genotype; 6, mixed infections (AI+AII); M, DNA ladder (B). Agarose gel electrophoresis of the semi-nested PCR amplification of a 476-bp of the tpi gene of the G. duodenalis assemblage A. Lanes 1, positive control; 2-6, patient's samples; 7, negative control (no DNA); M, DNA ladder (C). Digestion of PCR product targeting the tpi gene using Rsa I to distinguish AI (437-, 15-bp) and AII (232-, 235-, 15-bp) genotypes. Lane 1, undigested PCR product of the G. duodenalis assemblage A; 2-4; AI genotype; 5, AII genotype; 6 and 7, mixed infections (AI+AII); M, DNA ladder (D). Sizes in base pairs are denoted by the numbers at left.

    Article Snippet: Ten microliters of the PCR products resulting from the amplification of the gdh locus were digested with 5 U of BspL I ( Nla IV; Fermentas, St. Leon-Rot, Germany) in 1× Tango buffer for 2 h at 37°C in order to distinguish between assemblages A (AI/AII) and B.

    Techniques: Agarose Gel Electrophoresis, Nested PCR, Amplification, Negative Control, Positive Control, Polymerase Chain Reaction