ni-nta resin Qiagen Search Results


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  • 99
    Qiagen ni nta qiagen resin
    Coprecipitation of PDSS1 and PDSS2 polypeptides. His-PDSS2-L or His-PDSS2-L-V117M polypeptides were incubated with GST-PDSS1 for 30 min and subsequently precipitated by <t>Ni-NTA</t> <t>Superflow</t> resin ( lanes 2 and 3 ). A coprecipitation assay of GST and His-PDSS2-L
    Ni Nta Qiagen Resin, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 2693 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ni nta qiagen resin/product/Qiagen
    Average 99 stars, based on 2693 article reviews
    Price from $9.99 to $1999.99
    ni nta qiagen resin - by Bioz Stars, 2020-08
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    99
    Qiagen ni nta qiagen agarose resin
    SDS-PAGE of recombinant <t>FumF</t> protein . Proteins were separated by 12% (w/v) SDS-PAGE and then stained with Coomassie brilliant blue G-250. Lane 1, molecular weight standards; Lane 2, total protein of E. coli BL21(DE3)pLysS harboring empty pETBlue-2 (control); Lane 3, total protein of E. coli BL21(DE3)pLysS harboring the recombinant fumF in pETBlue-2 without induction by IPTG; Lane 4, total protein of E. coli BL21(DE3)pLysS harboring the recombinant fumF in pETBlue-2 induced by addition of 0.5 mM IPTG; Lane 5, sample purified by the <t>Ni-NTA</t> column method. The recombinant FumF protein is indicated by the black arrow.
    Ni Nta Qiagen Agarose Resin, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 646 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ni nta qiagen agarose resin/product/Qiagen
    Average 99 stars, based on 646 article reviews
    Price from $9.99 to $1999.99
    ni nta qiagen agarose resin - by Bioz Stars, 2020-08
    99/100 stars
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    90
    Qiagen ni nta qiagen resins
    SDS-PAGE of recombinant <t>FumF</t> protein . Proteins were separated by 12% (w/v) SDS-PAGE and then stained with Coomassie brilliant blue G-250. Lane 1, molecular weight standards; Lane 2, total protein of E. coli BL21(DE3)pLysS harboring empty pETBlue-2 (control); Lane 3, total protein of E. coli BL21(DE3)pLysS harboring the recombinant fumF in pETBlue-2 without induction by IPTG; Lane 4, total protein of E. coli BL21(DE3)pLysS harboring the recombinant fumF in pETBlue-2 induced by addition of 0.5 mM IPTG; Lane 5, sample purified by the <t>Ni-NTA</t> column method. The recombinant FumF protein is indicated by the black arrow.
    Ni Nta Qiagen Resins, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ni nta qiagen resins/product/Qiagen
    Average 90 stars, based on 22 article reviews
    Price from $9.99 to $1999.99
    ni nta qiagen resins - by Bioz Stars, 2020-08
    90/100 stars
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    94
    Qiagen assays ni nta agarose resin qiagen
    SDS-PAGE of recombinant <t>FumF</t> protein . Proteins were separated by 12% (w/v) SDS-PAGE and then stained with Coomassie brilliant blue G-250. Lane 1, molecular weight standards; Lane 2, total protein of E. coli BL21(DE3)pLysS harboring empty pETBlue-2 (control); Lane 3, total protein of E. coli BL21(DE3)pLysS harboring the recombinant fumF in pETBlue-2 without induction by IPTG; Lane 4, total protein of E. coli BL21(DE3)pLysS harboring the recombinant fumF in pETBlue-2 induced by addition of 0.5 mM IPTG; Lane 5, sample purified by the <t>Ni-NTA</t> column method. The recombinant FumF protein is indicated by the black arrow.
    Assays Ni Nta Agarose Resin Qiagen, supplied by Qiagen, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/assays ni nta agarose resin qiagen/product/Qiagen
    Average 94 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    assays ni nta agarose resin qiagen - by Bioz Stars, 2020-08
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    Image Search Results


    Coprecipitation of PDSS1 and PDSS2 polypeptides. His-PDSS2-L or His-PDSS2-L-V117M polypeptides were incubated with GST-PDSS1 for 30 min and subsequently precipitated by Ni-NTA Superflow resin ( lanes 2 and 3 ). A coprecipitation assay of GST and His-PDSS2-L

    Journal: American Journal of Physiology - Renal Physiology

    Article Title: Coenzyme Q10 supplementation rescues renal disease in Pdss2kd/kd mice with mutations in prenyl diphosphate synthase subunit 2

    doi: 10.1152/ajprenal.90445.2008

    Figure Lengend Snippet: Coprecipitation of PDSS1 and PDSS2 polypeptides. His-PDSS2-L or His-PDSS2-L-V117M polypeptides were incubated with GST-PDSS1 for 30 min and subsequently precipitated by Ni-NTA Superflow resin ( lanes 2 and 3 ). A coprecipitation assay of GST and His-PDSS2-L

    Article Snippet: The fusion protein was induced by 0.5 mM IPTG (Fisher Biotech) at 30°C for 3 h and purified from the bacterial lysate under denaturing conditions (8 M urea in 1× PBS, adjusted to pH 7.4) by Ni-NTA Superflow resin (Qiagen).

    Techniques: Incubation

    Formation of mixed oligomers between Cpn60.1 and Cpn60.3 in Escherichia coli . Crude extracts from strains expressing either Cpn60.1 alone, Cpn60.3 alone, Cpn60.1(his) 6 , or Cpn60.1(his) 6 and Cpn60.3, were passed through a Ni-NTA column and subsequently eluted with imidazole. Protein in the soluble supernatant, the initial wash through the column, and the eluted fraction, were detected using an anti-GroEL monoclonal antibody (4-3F) that cross-reacts equally well with Cpn60.1 and Cpn60.3

    Journal: Cell Stress & Chaperones

    Article Title: Homologous cpn60 genes in Rhizobium leguminosarum are not functionally equivalent

    doi: 10.1379/CSC-227R.1

    Figure Lengend Snippet: Formation of mixed oligomers between Cpn60.1 and Cpn60.3 in Escherichia coli . Crude extracts from strains expressing either Cpn60.1 alone, Cpn60.3 alone, Cpn60.1(his) 6 , or Cpn60.1(his) 6 and Cpn60.3, were passed through a Ni-NTA column and subsequently eluted with imidazole. Protein in the soluble supernatant, the initial wash through the column, and the eluted fraction, were detected using an anti-GroEL monoclonal antibody (4-3F) that cross-reacts equally well with Cpn60.1 and Cpn60.3

    Article Snippet: When the extract from this strain (grown on glucose to eliminate expression of the endogenous GroEL) was passed through Ni-NTA resin and bound proteins eluted with imidazole, a small amount of Cpn60.3 was detected in addition to the strong Cpn60.1 band, but the proportion of Cpn60.3 to Cpn60.1 was considerably lower than in the initial protein extract ( ).

    Techniques: Expressing

    SDS-PAGE of recombinant FumF protein . Proteins were separated by 12% (w/v) SDS-PAGE and then stained with Coomassie brilliant blue G-250. Lane 1, molecular weight standards; Lane 2, total protein of E. coli BL21(DE3)pLysS harboring empty pETBlue-2 (control); Lane 3, total protein of E. coli BL21(DE3)pLysS harboring the recombinant fumF in pETBlue-2 without induction by IPTG; Lane 4, total protein of E. coli BL21(DE3)pLysS harboring the recombinant fumF in pETBlue-2 induced by addition of 0.5 mM IPTG; Lane 5, sample purified by the Ni-NTA column method. The recombinant FumF protein is indicated by the black arrow.

    Journal: Microbial Cell Factories

    Article Title: Identification and characterization of a novel fumarase gene by metagenome expression cloning from marine microorganisms

    doi: 10.1186/1475-2859-9-91

    Figure Lengend Snippet: SDS-PAGE of recombinant FumF protein . Proteins were separated by 12% (w/v) SDS-PAGE and then stained with Coomassie brilliant blue G-250. Lane 1, molecular weight standards; Lane 2, total protein of E. coli BL21(DE3)pLysS harboring empty pETBlue-2 (control); Lane 3, total protein of E. coli BL21(DE3)pLysS harboring the recombinant fumF in pETBlue-2 without induction by IPTG; Lane 4, total protein of E. coli BL21(DE3)pLysS harboring the recombinant fumF in pETBlue-2 induced by addition of 0.5 mM IPTG; Lane 5, sample purified by the Ni-NTA column method. The recombinant FumF protein is indicated by the black arrow.

    Article Snippet: The His-tagged FumF protein was expressed and purified using Ni-NTA agarose resin (Qiagen, Valencia, CA, USA), according to the manufacturer's instructions.

    Techniques: SDS Page, Recombinant, Staining, Molecular Weight, Purification