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    Qiagen ni nta hissorb plates
    PEDF secretion by immortalized RPE cells in vitro and in vivo . (a) ELISA-based quantification and Western blot analysis of total PEDF secretion by immortalized rat RPE cells in vitro at passages 1, 2, 5, and 10 (grey bars) after SB100X -mediated transfection with the pT2-CMV-PEDF/EGFP transposon plasmid. Note that nontransfected control cells secreted only very small amounts of endogenous PEDF (white bar). Data represent the mean ± SD from 2 independent measurements ( ∗∗∗ p = 0.0001). (b) Western blot analysis of total PEDF extracted from rat control eyes and eyes at 1 day, 1, 2, and 3 weeks after subretinal transplantation of PEDF-transfected rat RPE cells. Double bands indicated the slightly different molecular weights of endogenous (46.5 kDa) and recombinant human PEDF (47.5 kDa), which included a His-tag. Loading of equal protein amounts was confirmed by similar densities of GAPDH protein bands (36 kDa). Note that for these experiments the number of cells transplanted was 1 × 10 5 , since the low sensitivity of the anti-PEDF antibodies required a larger amount of protein. Data represent the mean ± SD from 2 independent measurements. (c) ELISA-based quantification of recombinant PEDF secretion in vivo in lysates of rat eyes at 1 day, 1, 2, 3, and 4 weeks after subretinal transplantation of 1 × 10 4 PEDF-transfected RPE cells, showed a constant level of recombinant human PEDF. For nontransfected control cells recombinant PEDF was not detectable (left bar), because the analysis was carried out with <t>Ni-NTA</t> <t>HisSorb</t> plates. Each bar represents the average data of three injected eyes ( ∗∗∗∗ p
    Ni Nta Hissorb Plates, supplied by Qiagen, used in various techniques. Bioz Stars score: 97/100, based on 124 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ni nta hissorb plates/product/Qiagen
    Average 97 stars, based on 124 article reviews
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    ni nta hissorb plates - by Bioz Stars, 2020-09
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    94
    Qiagen ni nta hissorb strips
    PEDF secretion by immortalized RPE cells in vitro and in vivo . (a) ELISA-based quantification and Western blot analysis of total PEDF secretion by immortalized rat RPE cells in vitro at passages 1, 2, 5, and 10 (grey bars) after SB100X -mediated transfection with the pT2-CMV-PEDF/EGFP transposon plasmid. Note that nontransfected control cells secreted only very small amounts of endogenous PEDF (white bar). Data represent the mean ± SD from 2 independent measurements ( ∗∗∗ p = 0.0001). (b) Western blot analysis of total PEDF extracted from rat control eyes and eyes at 1 day, 1, 2, and 3 weeks after subretinal transplantation of PEDF-transfected rat RPE cells. Double bands indicated the slightly different molecular weights of endogenous (46.5 kDa) and recombinant human PEDF (47.5 kDa), which included a His-tag. Loading of equal protein amounts was confirmed by similar densities of GAPDH protein bands (36 kDa). Note that for these experiments the number of cells transplanted was 1 × 10 5 , since the low sensitivity of the anti-PEDF antibodies required a larger amount of protein. Data represent the mean ± SD from 2 independent measurements. (c) ELISA-based quantification of recombinant PEDF secretion in vivo in lysates of rat eyes at 1 day, 1, 2, 3, and 4 weeks after subretinal transplantation of 1 × 10 4 PEDF-transfected RPE cells, showed a constant level of recombinant human PEDF. For nontransfected control cells recombinant PEDF was not detectable (left bar), because the analysis was carried out with <t>Ni-NTA</t> <t>HisSorb</t> plates. Each bar represents the average data of three injected eyes ( ∗∗∗∗ p
    Ni Nta Hissorb Strips, supplied by Qiagen, used in various techniques. Bioz Stars score: 94/100, based on 854 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ni nta hissorb strips/product/Qiagen
    Average 94 stars, based on 854 article reviews
    Price from $9.99 to $1999.99
    ni nta hissorb strips - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    PEDF secretion by immortalized RPE cells in vitro and in vivo . (a) ELISA-based quantification and Western blot analysis of total PEDF secretion by immortalized rat RPE cells in vitro at passages 1, 2, 5, and 10 (grey bars) after SB100X -mediated transfection with the pT2-CMV-PEDF/EGFP transposon plasmid. Note that nontransfected control cells secreted only very small amounts of endogenous PEDF (white bar). Data represent the mean ± SD from 2 independent measurements ( ∗∗∗ p = 0.0001). (b) Western blot analysis of total PEDF extracted from rat control eyes and eyes at 1 day, 1, 2, and 3 weeks after subretinal transplantation of PEDF-transfected rat RPE cells. Double bands indicated the slightly different molecular weights of endogenous (46.5 kDa) and recombinant human PEDF (47.5 kDa), which included a His-tag. Loading of equal protein amounts was confirmed by similar densities of GAPDH protein bands (36 kDa). Note that for these experiments the number of cells transplanted was 1 × 10 5 , since the low sensitivity of the anti-PEDF antibodies required a larger amount of protein. Data represent the mean ± SD from 2 independent measurements. (c) ELISA-based quantification of recombinant PEDF secretion in vivo in lysates of rat eyes at 1 day, 1, 2, 3, and 4 weeks after subretinal transplantation of 1 × 10 4 PEDF-transfected RPE cells, showed a constant level of recombinant human PEDF. For nontransfected control cells recombinant PEDF was not detectable (left bar), because the analysis was carried out with Ni-NTA HisSorb plates. Each bar represents the average data of three injected eyes ( ∗∗∗∗ p

    Journal: BioMed Research International

    Article Title: Antiangiogenic and Neurogenic Activities of Sleeping Beauty-Mediated PEDF-Transfected RPE Cells In Vitro and In Vivo

    doi: 10.1155/2015/863845

    Figure Lengend Snippet: PEDF secretion by immortalized RPE cells in vitro and in vivo . (a) ELISA-based quantification and Western blot analysis of total PEDF secretion by immortalized rat RPE cells in vitro at passages 1, 2, 5, and 10 (grey bars) after SB100X -mediated transfection with the pT2-CMV-PEDF/EGFP transposon plasmid. Note that nontransfected control cells secreted only very small amounts of endogenous PEDF (white bar). Data represent the mean ± SD from 2 independent measurements ( ∗∗∗ p = 0.0001). (b) Western blot analysis of total PEDF extracted from rat control eyes and eyes at 1 day, 1, 2, and 3 weeks after subretinal transplantation of PEDF-transfected rat RPE cells. Double bands indicated the slightly different molecular weights of endogenous (46.5 kDa) and recombinant human PEDF (47.5 kDa), which included a His-tag. Loading of equal protein amounts was confirmed by similar densities of GAPDH protein bands (36 kDa). Note that for these experiments the number of cells transplanted was 1 × 10 5 , since the low sensitivity of the anti-PEDF antibodies required a larger amount of protein. Data represent the mean ± SD from 2 independent measurements. (c) ELISA-based quantification of recombinant PEDF secretion in vivo in lysates of rat eyes at 1 day, 1, 2, 3, and 4 weeks after subretinal transplantation of 1 × 10 4 PEDF-transfected RPE cells, showed a constant level of recombinant human PEDF. For nontransfected control cells recombinant PEDF was not detectable (left bar), because the analysis was carried out with Ni-NTA HisSorb plates. Each bar represents the average data of three injected eyes ( ∗∗∗∗ p

    Article Snippet: ELISA-based quantification was performed using the ELISAquant kit for human PEDF (BioProducts MD) and Ni-NTA HisSorb plates (Qiagen) according to the manufacturer's instructions.

    Techniques: In Vitro, In Vivo, Enzyme-linked Immunosorbent Assay, Western Blot, Transfection, Plasmid Preparation, Transplantation Assay, Recombinant, Injection

    Confirmation of predicted cleavage site proteolysis by HDM proteases. Cleavage efficiencies of the predicted sites present at the 21 potential targets (ΔA/A) by Der p 1, Der p 3 and Der p 6 are represented in blue, red and green, respectively. Individual purified phages were either untreated (A) or treated (∆A) with Der p 1, Der p 3 or Der p 6 for 30 min at 37 °C before ELISA on Ni-NTA HisSorb plates. Data presented as mean of two independent experiments performed in duplicate ± standard error of the mean. Significance was calculated compared to negative control using a Mann-Whitney test ( p value 0.05).

    Journal: International Journal of Molecular Sciences

    Article Title: Profiling the Extended Cleavage Specificity of the House Dust Mite Protease Allergens Der p 1, Der p 3 and Der p 6 for the Prediction of New Cell Surface Protein Substrates

    doi: 10.3390/ijms18071373

    Figure Lengend Snippet: Confirmation of predicted cleavage site proteolysis by HDM proteases. Cleavage efficiencies of the predicted sites present at the 21 potential targets (ΔA/A) by Der p 1, Der p 3 and Der p 6 are represented in blue, red and green, respectively. Individual purified phages were either untreated (A) or treated (∆A) with Der p 1, Der p 3 or Der p 6 for 30 min at 37 °C before ELISA on Ni-NTA HisSorb plates. Data presented as mean of two independent experiments performed in duplicate ± standard error of the mean. Significance was calculated compared to negative control using a Mann-Whitney test ( p value 0.05).

    Article Snippet: Phages were then immobilized for 2 h at 25 °C in 96-well Ni-NTA HisSorb plates (Qiagen).

    Techniques: Purification, Enzyme-linked Immunosorbent Assay, Negative Control, MANN-WHITNEY