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  • 98
    Thermo Fisher nhei site italicized
    Nhei Site Italicized, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nhei site italicized/product/Thermo Fisher
    Average 98 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    nhei site italicized - by Bioz Stars, 2020-08
    98/100 stars
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    89
    Promega nhei site
    Sequence elements facilitating the subcloning procedure . (A) A double stranded oligonucleotide was cloned into <t>pGEM-T</t> easy to provide two key elements flanking a BglII site. We inserted a 1166 bp PCR amplified fragment into this restriction site. In future applications this step may as well be achieved by TA cloning or other methods. (B) A key element consists of a Esp3I recognition site and a restriction site generating a <t>NheI</t> compatible overhang, that is not recognized by NheI. (C) We used NheI as target recognition site in the destination vector.
    Nhei Site, supplied by Promega, used in various techniques. Bioz Stars score: 89/100, based on 136 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nhei site/product/Promega
    Average 89 stars, based on 136 article reviews
    Price from $9.99 to $1999.99
    nhei site - by Bioz Stars, 2020-08
    89/100 stars
      Buy from Supplier

    91
    System Biosciences Inc nhei site
    Sequence elements facilitating the subcloning procedure . (A) A double stranded oligonucleotide was cloned into <t>pGEM-T</t> easy to provide two key elements flanking a BglII site. We inserted a 1166 bp PCR amplified fragment into this restriction site. In future applications this step may as well be achieved by TA cloning or other methods. (B) A key element consists of a Esp3I recognition site and a restriction site generating a <t>NheI</t> compatible overhang, that is not recognized by NheI. (C) We used NheI as target recognition site in the destination vector.
    Nhei Site, supplied by System Biosciences Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nhei site/product/System Biosciences Inc
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nhei site - by Bioz Stars, 2020-08
    91/100 stars
      Buy from Supplier

    89
    Stratagene nhei site
    Sequence elements facilitating the subcloning procedure . (A) A double stranded oligonucleotide was cloned into <t>pGEM-T</t> easy to provide two key elements flanking a BglII site. We inserted a 1166 bp PCR amplified fragment into this restriction site. In future applications this step may as well be achieved by TA cloning or other methods. (B) A key element consists of a Esp3I recognition site and a restriction site generating a <t>NheI</t> compatible overhang, that is not recognized by NheI. (C) We used NheI as target recognition site in the destination vector.
    Nhei Site, supplied by Stratagene, used in various techniques. Bioz Stars score: 89/100, based on 58 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nhei site/product/Stratagene
    Average 89 stars, based on 58 article reviews
    Price from $9.99 to $1999.99
    nhei site - by Bioz Stars, 2020-08
    89/100 stars
      Buy from Supplier

    Image Search Results


    Sequence elements facilitating the subcloning procedure . (A) A double stranded oligonucleotide was cloned into pGEM-T easy to provide two key elements flanking a BglII site. We inserted a 1166 bp PCR amplified fragment into this restriction site. In future applications this step may as well be achieved by TA cloning or other methods. (B) A key element consists of a Esp3I recognition site and a restriction site generating a NheI compatible overhang, that is not recognized by NheI. (C) We used NheI as target recognition site in the destination vector.

    Journal: Journal of Biological Engineering

    Article Title: Rapid single step subcloning procedure by combined action of type II and type IIs endonucleases with ligase

    doi: 10.1186/1754-1611-1-7

    Figure Lengend Snippet: Sequence elements facilitating the subcloning procedure . (A) A double stranded oligonucleotide was cloned into pGEM-T easy to provide two key elements flanking a BglII site. We inserted a 1166 bp PCR amplified fragment into this restriction site. In future applications this step may as well be achieved by TA cloning or other methods. (B) A key element consists of a Esp3I recognition site and a restriction site generating a NheI compatible overhang, that is not recognized by NheI. (C) We used NheI as target recognition site in the destination vector.

    Article Snippet: Method In an example experiment we subcloned a 1166 bp long DNA fragment* from an entry vector (modified pGEM-T easy, Promega) into a NheI site of a destination vector (pEGFP-N1, Clontech).

    Techniques: Sequencing, Subcloning, Clone Assay, Polymerase Chain Reaction, Amplification, TA Cloning, Plasmid Preparation