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  • 99
    New England Biolabs nhei
    Electrophoresis of pPrime- fliC Recombinant Plasmids From White Colonies on 1% Agarose Gel Lane 1 to 6: extracted Pprime- fliC plasmids; Lane 8 to 13: Pprime- fliC plasmids digested by <t>XhoI;</t> Lane 15 to 20: Pprime- fliC plasmids digested by XhoI and <t>NheI;</t> Lane 7 and 14: 1 Kbp DNA ladder vivantis.
    Nhei, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 3400 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher nhei
    Digestion of 746-bp 23S rRNA obtained by <t>PCR</t> with <t>NheI</t> and separated on 2% agarose gel in 1× Tris-acetate-EDTA buffer. Lane 1, φX174 replicative-form DNA/HaeIII molecular mass standard; lanes 2 and 4, linezolid-susceptible strains SM888 and SM913 (MIC, 2 μg/ml); lanes 3 and 5, strains SM912 and SM925 (MIC, 8 μg/ml); lanes 6 and 7, strains SM943 and SM944 (MIC, 64 μg/ml).
    Nhei, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2924 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Promega nhei
    700TKLuc retains strong transcriptional activation in a keratinocyte-specific fashion in vitro (A) Diagram of the five initial luciferase constructs used for experiments shown in panels B to D. The partial restriction map of the K14 5′ upstream region shown includes the Ava I (+1), <t>Nhe</t> I (−350), Eco RV (−1000), Bbs I (−1570), and Hin dIII (−2200) sites and the approximate positions of HSsII and HSsIII. (B) Luciferase activity data from primary human keratinocytes transfected with constructs shown in panel A. The value for the TKLuc construct was low and was arbitrarily set at 1. (C) Activity of the 700-bp enhancer (−2000 to −1300) in conjunction with different heterologous promoters. Activities of all the minimal promoters were low and set at 1. SV40, major early viral promoter; K14mp, K14 minimal promoter; TK, thymidine kinase minimal promoter. (D) Activity of 700TKLuc in different cell types and/or lines. hK, primary human keratinocytes; mK, immortalized mouse keratinocytes. In all transfection experiments, luciferase activities were corrected for β-galactosidase values from an internal control reporter, CMV- lacZ . Note that β-galactosidase activities were comparable in all lines tested. Activities represent the mean ± standard deviation of a minimum of three different experiments performed in duplicate.
    Nhei, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 2192 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nhei  (TaKaRa)
    99
    TaKaRa nhei
    700TKLuc retains strong transcriptional activation in a keratinocyte-specific fashion in vitro (A) Diagram of the five initial luciferase constructs used for experiments shown in panels B to D. The partial restriction map of the K14 5′ upstream region shown includes the Ava I (+1), <t>Nhe</t> I (−350), Eco RV (−1000), Bbs I (−1570), and Hin dIII (−2200) sites and the approximate positions of HSsII and HSsIII. (B) Luciferase activity data from primary human keratinocytes transfected with constructs shown in panel A. The value for the TKLuc construct was low and was arbitrarily set at 1. (C) Activity of the 700-bp enhancer (−2000 to −1300) in conjunction with different heterologous promoters. Activities of all the minimal promoters were low and set at 1. SV40, major early viral promoter; K14mp, K14 minimal promoter; TK, thymidine kinase minimal promoter. (D) Activity of 700TKLuc in different cell types and/or lines. hK, primary human keratinocytes; mK, immortalized mouse keratinocytes. In all transfection experiments, luciferase activities were corrected for β-galactosidase values from an internal control reporter, CMV- lacZ . Note that β-galactosidase activities were comparable in all lines tested. Activities represent the mean ± standard deviation of a minimum of three different experiments performed in duplicate.
    Nhei, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1827 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore nhei
    Linear view of consensus optical maps with <t>DNA</t> sequence-based maps. Solid black arrows represent the locations of missing cuts in the consensus optical maps. The XbaI and <t>NheI</t> consensus optical maps extend to the left of the origin of the DNA sequence-based map because both optical maps had a missing cut at that position.
    Nhei, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1143 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    New England Biolabs nhei hf
    Linear view of consensus optical maps with <t>DNA</t> sequence-based maps. Solid black arrows represent the locations of missing cuts in the consensus optical maps. The XbaI and <t>NheI</t> consensus optical maps extend to the left of the origin of the DNA sequence-based map because both optical maps had a missing cut at that position.
    Nhei Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 518 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Thermo Fisher fastdigest nhei
    Linear view of consensus optical maps with <t>DNA</t> sequence-based maps. Solid black arrows represent the locations of missing cuts in the consensus optical maps. The XbaI and <t>NheI</t> consensus optical maps extend to the left of the origin of the DNA sequence-based map because both optical maps had a missing cut at that position.
    Fastdigest Nhei, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 121 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher nhei hindiii
    Linear view of consensus optical maps with <t>DNA</t> sequence-based maps. Solid black arrows represent the locations of missing cuts in the consensus optical maps. The XbaI and <t>NheI</t> consensus optical maps extend to the left of the origin of the DNA sequence-based map because both optical maps had a missing cut at that position.
    Nhei Hindiii, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher nhei bamhi sites
    Linear view of consensus optical maps with <t>DNA</t> sequence-based maps. Solid black arrows represent the locations of missing cuts in the consensus optical maps. The XbaI and <t>NheI</t> consensus optical maps extend to the left of the origin of the DNA sequence-based map because both optical maps had a missing cut at that position.
    Nhei Bamhi Sites, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Shanghai Genechem agei nhei
    Linear view of consensus optical maps with <t>DNA</t> sequence-based maps. Solid black arrows represent the locations of missing cuts in the consensus optical maps. The XbaI and <t>NheI</t> consensus optical maps extend to the left of the origin of the DNA sequence-based map because both optical maps had a missing cut at that position.
    Agei Nhei, supplied by Shanghai Genechem, used in various techniques. Bioz Stars score: 92/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Addgene inc ecori nhei digested plv teto hngn2 p2a egfp t2a puro
    Linear view of consensus optical maps with <t>DNA</t> sequence-based maps. Solid black arrows represent the locations of missing cuts in the consensus optical maps. The XbaI and <t>NheI</t> consensus optical maps extend to the left of the origin of the DNA sequence-based map because both optical maps had a missing cut at that position.
    Ecori Nhei Digested Plv Teto Hngn2 P2a Egfp T2a Puro, supplied by Addgene inc, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Electrophoresis of pPrime- fliC Recombinant Plasmids From White Colonies on 1% Agarose Gel Lane 1 to 6: extracted Pprime- fliC plasmids; Lane 8 to 13: Pprime- fliC plasmids digested by XhoI; Lane 15 to 20: Pprime- fliC plasmids digested by XhoI and NheI; Lane 7 and 14: 1 Kbp DNA ladder vivantis.

    Journal: Jundishapur Journal of Microbiology

    Article Title: Cloning of fliC Gene From Salmonella typhimurium in the Expression Vector pVAX1 and Evaluation of its Expression in Eukaryotic Cells

    doi: 10.5812/jjm.12351

    Figure Lengend Snippet: Electrophoresis of pPrime- fliC Recombinant Plasmids From White Colonies on 1% Agarose Gel Lane 1 to 6: extracted Pprime- fliC plasmids; Lane 8 to 13: Pprime- fliC plasmids digested by XhoI; Lane 15 to 20: Pprime- fliC plasmids digested by XhoI and NheI; Lane 7 and 14: 1 Kbp DNA ladder vivantis.

    Article Snippet: The recombinant plasmids were detected by restriction digestion with NheI and XhoI enzymes (New England BioLabs, USA) and confirmed by sequence analysis (Bioneer, Korea).

    Techniques: Electrophoresis, Recombinant, Agarose Gel Electrophoresis

    AgaroseGel Electrophoresis of Restriction Enzyme Digestion of Purified pVAX- fliC Recombinant Plasmid Lane 1: pVAX- fliC plasmid digested by XhoI had one band (4419 bp); Lane 2: Double Digestion by NheI and XhoI on pVAX- fliC plasmid had two bands that were 1509 bp (down) and 2910 bp (up); Lane 3: 1 Kbp DNA ladder Vivantis.

    Journal: Jundishapur Journal of Microbiology

    Article Title: Cloning of fliC Gene From Salmonella typhimurium in the Expression Vector pVAX1 and Evaluation of its Expression in Eukaryotic Cells

    doi: 10.5812/jjm.12351

    Figure Lengend Snippet: AgaroseGel Electrophoresis of Restriction Enzyme Digestion of Purified pVAX- fliC Recombinant Plasmid Lane 1: pVAX- fliC plasmid digested by XhoI had one band (4419 bp); Lane 2: Double Digestion by NheI and XhoI on pVAX- fliC plasmid had two bands that were 1509 bp (down) and 2910 bp (up); Lane 3: 1 Kbp DNA ladder Vivantis.

    Article Snippet: The recombinant plasmids were detected by restriction digestion with NheI and XhoI enzymes (New England BioLabs, USA) and confirmed by sequence analysis (Bioneer, Korea).

    Techniques: Electrophoresis, Purification, Recombinant, Plasmid Preparation

    Dendrogram based on Nhe I PFGE patterns of 40 N. gonorrhoeae isolates.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Molecular Basis of High-Level Ciprofloxacin Resistance in Neisseria gonorrhoeae Strains Isolated in Denmark from 1995 to 1998

    doi: 10.1128/AAC.45.1.117-123.2001

    Figure Lengend Snippet: Dendrogram based on Nhe I PFGE patterns of 40 N. gonorrhoeae isolates.

    Article Snippet: Slices of DNA-containing agarose blocks were digested with Spe I and Nhe I (New England Biolabs) overnight at 37°C in 100 μl of restriction endonuclease buffer containing 10 U of enzyme.

    Techniques:

    Restriction maps of plasmid and bacteriophage DNA obtained from V. parahaemolyticus strain PMC 58.5. N, H, and S correspond to NheI, HindIII, and SphI restriction enzymes sites, respectively. A hypothetical circular map is shown in the middle, with the possible ends shown with a red arrow and the restriction sites with a black line. b, bacteriophage; p, plasmid.

    Journal: Applied and Environmental Microbiology

    Article Title: Enhancement of UV Light Sensitivity of a Vibrio parahaemolyticus O3:K6 Pandemic Strain Due to Natural Lysogenization by a Telomeric Phage ▿

    doi: 10.1128/AEM.01995-08

    Figure Lengend Snippet: Restriction maps of plasmid and bacteriophage DNA obtained from V. parahaemolyticus strain PMC 58.5. N, H, and S correspond to NheI, HindIII, and SphI restriction enzymes sites, respectively. A hypothetical circular map is shown in the middle, with the possible ends shown with a red arrow and the restriction sites with a black line. b, bacteriophage; p, plasmid.

    Article Snippet: Restriction site mapping was performed by digestion with restriction enzymes NheI, HindIII, and SphI from New England Biolabs (Massachusetts), according to the manufacturer's instructions.

    Techniques: Plasmid Preparation

    Digestion of 746-bp 23S rRNA obtained by PCR with NheI and separated on 2% agarose gel in 1× Tris-acetate-EDTA buffer. Lane 1, φX174 replicative-form DNA/HaeIII molecular mass standard; lanes 2 and 4, linezolid-susceptible strains SM888 and SM913 (MIC, 2 μg/ml); lanes 3 and 5, strains SM912 and SM925 (MIC, 8 μg/ml); lanes 6 and 7, strains SM943 and SM944 (MIC, 64 μg/ml).

    Journal: Journal of Clinical Microbiology

    Article Title: Emergence of Linezolid Resistance in the Vancomycin-Resistant Enterococcus faecium Multilocus Sequence Typing C1 Epidemic Lineage

    doi: 10.1128/JCM.44.3.1153-1155.2006

    Figure Lengend Snippet: Digestion of 746-bp 23S rRNA obtained by PCR with NheI and separated on 2% agarose gel in 1× Tris-acetate-EDTA buffer. Lane 1, φX174 replicative-form DNA/HaeIII molecular mass standard; lanes 2 and 4, linezolid-susceptible strains SM888 and SM913 (MIC, 2 μg/ml); lanes 3 and 5, strains SM912 and SM925 (MIC, 8 μg/ml); lanes 6 and 7, strains SM943 and SM944 (MIC, 64 μg/ml).

    Article Snippet: The cycling conditions were as follows: an initial denaturation at 94°C for 5 min, followed by 30 cycles consisting of 94°C for 1 min, 50°C for 30 s, 72°C for 1 min, and a final extension step at 72°C for 5 min. Small amounts (5 μl) of PCR 23S rRNA were digested with 10 units of NheI (Invitrogen Life Technologies) at 37°C for 4 h and separated on 2% agarose gel in 1× Tris-acetate-EDTA buffer.

    Techniques: Polymerase Chain Reaction, Agarose Gel Electrophoresis

    700TKLuc retains strong transcriptional activation in a keratinocyte-specific fashion in vitro (A) Diagram of the five initial luciferase constructs used for experiments shown in panels B to D. The partial restriction map of the K14 5′ upstream region shown includes the Ava I (+1), Nhe I (−350), Eco RV (−1000), Bbs I (−1570), and Hin dIII (−2200) sites and the approximate positions of HSsII and HSsIII. (B) Luciferase activity data from primary human keratinocytes transfected with constructs shown in panel A. The value for the TKLuc construct was low and was arbitrarily set at 1. (C) Activity of the 700-bp enhancer (−2000 to −1300) in conjunction with different heterologous promoters. Activities of all the minimal promoters were low and set at 1. SV40, major early viral promoter; K14mp, K14 minimal promoter; TK, thymidine kinase minimal promoter. (D) Activity of 700TKLuc in different cell types and/or lines. hK, primary human keratinocytes; mK, immortalized mouse keratinocytes. In all transfection experiments, luciferase activities were corrected for β-galactosidase values from an internal control reporter, CMV- lacZ . Note that β-galactosidase activities were comparable in all lines tested. Activities represent the mean ± standard deviation of a minimum of three different experiments performed in duplicate.

    Journal: Molecular and Cellular Biology

    Article Title: Defining the Regulatory Factors Required for Epidermal Gene Expression

    doi:

    Figure Lengend Snippet: 700TKLuc retains strong transcriptional activation in a keratinocyte-specific fashion in vitro (A) Diagram of the five initial luciferase constructs used for experiments shown in panels B to D. The partial restriction map of the K14 5′ upstream region shown includes the Ava I (+1), Nhe I (−350), Eco RV (−1000), Bbs I (−1570), and Hin dIII (−2200) sites and the approximate positions of HSsII and HSsIII. (B) Luciferase activity data from primary human keratinocytes transfected with constructs shown in panel A. The value for the TKLuc construct was low and was arbitrarily set at 1. (C) Activity of the 700-bp enhancer (−2000 to −1300) in conjunction with different heterologous promoters. Activities of all the minimal promoters were low and set at 1. SV40, major early viral promoter; K14mp, K14 minimal promoter; TK, thymidine kinase minimal promoter. (D) Activity of 700TKLuc in different cell types and/or lines. hK, primary human keratinocytes; mK, immortalized mouse keratinocytes. In all transfection experiments, luciferase activities were corrected for β-galactosidase values from an internal control reporter, CMV- lacZ . Note that β-galactosidase activities were comparable in all lines tested. Activities represent the mean ± standard deviation of a minimum of three different experiments performed in duplicate.

    Article Snippet: The thymidine kinase (TK) minimal promoter ( ) was cloned into the Nhe I and Xho I sites of the luciferase reporter pGL3-basic vector (Promega) to create the LTK construct.

    Techniques: Activation Assay, In Vitro, Luciferase, Construct, Antiviral Assay, Activity Assay, Transfection, Standard Deviation

    Linear view of consensus optical maps with DNA sequence-based maps. Solid black arrows represent the locations of missing cuts in the consensus optical maps. The XbaI and NheI consensus optical maps extend to the left of the origin of the DNA sequence-based map because both optical maps had a missing cut at that position.

    Journal: Applied and Environmental Microbiology

    Article Title: Whole-Genome Shotgun Optical Mapping of Rhodospirillum rubrum

    doi: 10.1128/AEM.71.9.5511-5522.2005

    Figure Lengend Snippet: Linear view of consensus optical maps with DNA sequence-based maps. Solid black arrows represent the locations of missing cuts in the consensus optical maps. The XbaI and NheI consensus optical maps extend to the left of the origin of the DNA sequence-based map because both optical maps had a missing cut at that position.

    Article Snippet: Surface properties were assayed by digesting lambda DASH II bacteriophage DNA with 40 units of XbaI, HindIII, and NheI diluted in 200 μl of digestion buffer with 0.2% Triton X-100 (Sigma, St. Louis, MO) at 37°C to determine optimal digestion times, which ranged from 30 to 120 min.

    Techniques: Sequencing

    Use of optical maps in confirming assembly and order of sequence contigs. In silico maps of the sequence contigs were made and aligned against the whole-genome optical maps located in the center of each diagram. A) The high-resolution HindIII map enabled alignment of the small (∼80 kb) sequence contig (contig 83) between contigs 85c and 86c. Aside from the redundant alignment of contigs 87 and 90, no gaps remained in alignment between the optical map and sequence. B) The NheI map gave the clearest representation of the redundant alignment, shown in red in the optical map, of contigs 87 and 90. The alignment of contig 87 had a better match to the optical map than do the rightmost eight fragments in contig 90 (shown in the red square). C) Removing the rightmost eight fragments from contig 90 to make contigs 90a and 90b and reversing the orientation of 90b permitted alignment to the gap between contig 87 and contig 85c. Alignments were made with MapViewer Software (OpGen, Inc, Madison WI.).

    Journal: Applied and Environmental Microbiology

    Article Title: Whole-Genome Shotgun Optical Mapping of Rhodospirillum rubrum

    doi: 10.1128/AEM.71.9.5511-5522.2005

    Figure Lengend Snippet: Use of optical maps in confirming assembly and order of sequence contigs. In silico maps of the sequence contigs were made and aligned against the whole-genome optical maps located in the center of each diagram. A) The high-resolution HindIII map enabled alignment of the small (∼80 kb) sequence contig (contig 83) between contigs 85c and 86c. Aside from the redundant alignment of contigs 87 and 90, no gaps remained in alignment between the optical map and sequence. B) The NheI map gave the clearest representation of the redundant alignment, shown in red in the optical map, of contigs 87 and 90. The alignment of contig 87 had a better match to the optical map than do the rightmost eight fragments in contig 90 (shown in the red square). C) Removing the rightmost eight fragments from contig 90 to make contigs 90a and 90b and reversing the orientation of 90b permitted alignment to the gap between contig 87 and contig 85c. Alignments were made with MapViewer Software (OpGen, Inc, Madison WI.).

    Article Snippet: Surface properties were assayed by digesting lambda DASH II bacteriophage DNA with 40 units of XbaI, HindIII, and NheI diluted in 200 μl of digestion buffer with 0.2% Triton X-100 (Sigma, St. Louis, MO) at 37°C to determine optimal digestion times, which ranged from 30 to 120 min.

    Techniques: Sequencing, In Silico, Software

    Comparisons of the XbaI, NheI, and HindIII optical maps to sequence data. (A, B, and C) Plots of optical map fragment sizes versus the DNA sequence-based map fragment sizes from the finished sequence for XbaI (A), NheI (B), and HindIII (C). The error bars represent the standard deviation of optical map fragment size about the mean. (D, E, F) Plots of the relative fragment sizing error [100 × (optical map fragment size - corresponding DNA sequence-based map fragment size)/corresponding DNA sequence-based map fragment size] versus DNA sequence-based map fragment size for XbaI ( D), NheI (E), and HindIII (F).

    Journal: Applied and Environmental Microbiology

    Article Title: Whole-Genome Shotgun Optical Mapping of Rhodospirillum rubrum

    doi: 10.1128/AEM.71.9.5511-5522.2005

    Figure Lengend Snippet: Comparisons of the XbaI, NheI, and HindIII optical maps to sequence data. (A, B, and C) Plots of optical map fragment sizes versus the DNA sequence-based map fragment sizes from the finished sequence for XbaI (A), NheI (B), and HindIII (C). The error bars represent the standard deviation of optical map fragment size about the mean. (D, E, F) Plots of the relative fragment sizing error [100 × (optical map fragment size - corresponding DNA sequence-based map fragment size)/corresponding DNA sequence-based map fragment size] versus DNA sequence-based map fragment size for XbaI ( D), NheI (E), and HindIII (F).

    Article Snippet: Surface properties were assayed by digesting lambda DASH II bacteriophage DNA with 40 units of XbaI, HindIII, and NheI diluted in 200 μl of digestion buffer with 0.2% Triton X-100 (Sigma, St. Louis, MO) at 37°C to determine optimal digestion times, which ranged from 30 to 120 min.

    Techniques: Sequencing, Standard Deviation

    Cloning of HSA . a 1% agarose gel showing amplified PCR product and pET23b vector. Lane M, 1 kb DNA ladder; Lane 1, Uncut pET23b plasmid; Lane 2, a double digested pET23b vector with Nhe I and Xho I restriction enzymes; Lane 3, PCR amplified a product of HSA gene at an optimized annealing temperature of 58 °C. b 1% agarose gel showing the release of HSA gene after double digestion of constructed pETHSA vector with Nhe I and Xho I restriction enzymes. Lane M, 1 kb DNA ladder; Lane 1, Undigested pETHSA plasmid; Lane 2, digested pETHSA construct. c 12% SDS PAGE showing expression of rHSA in different strains of E. coli . Lane UI, Uninduced cells; Lane 1, Induced BL21 (DE3) E. coli cells; Lane 2, Induced Rosetta (DE3) E. coli cells; Lane 3, Induced Origami2 (DE3) E. coli cells

    Journal: Microbial Cell Factories

    Article Title: Revisiting Escherichia coli as microbial factory for enhanced production of human serum albumin

    doi: 10.1186/s12934-017-0784-8

    Figure Lengend Snippet: Cloning of HSA . a 1% agarose gel showing amplified PCR product and pET23b vector. Lane M, 1 kb DNA ladder; Lane 1, Uncut pET23b plasmid; Lane 2, a double digested pET23b vector with Nhe I and Xho I restriction enzymes; Lane 3, PCR amplified a product of HSA gene at an optimized annealing temperature of 58 °C. b 1% agarose gel showing the release of HSA gene after double digestion of constructed pETHSA vector with Nhe I and Xho I restriction enzymes. Lane M, 1 kb DNA ladder; Lane 1, Undigested pETHSA plasmid; Lane 2, digested pETHSA construct. c 12% SDS PAGE showing expression of rHSA in different strains of E. coli . Lane UI, Uninduced cells; Lane 1, Induced BL21 (DE3) E. coli cells; Lane 2, Induced Rosetta (DE3) E. coli cells; Lane 3, Induced Origami2 (DE3) E. coli cells

    Article Snippet: The PCR fragments were double-digested with Nhe I and Xho I and then subcloned into a pET23b expression vector (Novagen, USA) that had been pre-digested with the same enzymes.

    Techniques: Clone Assay, Agarose Gel Electrophoresis, Amplification, Polymerase Chain Reaction, Plasmid Preparation, Construct, SDS Page, Expressing

    CU-NP inhibits NHE-1 expression and activity and reduces [Na + ] i in hypertrophied cardiomyocytes. ( A and B ) The effect of CU-NP on NHE-1 mRNA and protein expression, respectively, after treatments, whereas ( C ) to ( E ) show pH i recoveries after NH 4 Cl pulsing.

    Journal: Cardiovascular Research

    Article Title: A novel chimeric natriuretic peptide reduces cardiomyocyte hypertrophy through the NHE-1-calcineurin pathway

    doi: 10.1093/cvr/cvq254

    Figure Lengend Snippet: CU-NP inhibits NHE-1 expression and activity and reduces [Na + ] i in hypertrophied cardiomyocytes. ( A and B ) The effect of CU-NP on NHE-1 mRNA and protein expression, respectively, after treatments, whereas ( C ) to ( E ) show pH i recoveries after NH 4 Cl pulsing.

    Article Snippet: The expression levels of ANP, α-skeletal actin, NHE-1, MCIP, and 18S rRNA genes were determined in 10 µL reaction volume using SYBR Green Jumpstart Taq ReadyMix DNA polymerase (Sigma-Aldrich), and fluorescence was measured and quantified using a DNA Engine Opticon 2 System (MJ Research, Waltham, MA, USA).

    Techniques: Expressing, Activity Assay

    Potential pathway underlying the anti-hypertrophic effect of CU-NP. As illustrated in ( A ), hypertrophic stimuli including PE, Ang II, and ET-1 activate NHE-1 via stimulation of their respective receptors (R). NHE-1 activation and the resultant increase

    Journal: Cardiovascular Research

    Article Title: A novel chimeric natriuretic peptide reduces cardiomyocyte hypertrophy through the NHE-1-calcineurin pathway

    doi: 10.1093/cvr/cvq254

    Figure Lengend Snippet: Potential pathway underlying the anti-hypertrophic effect of CU-NP. As illustrated in ( A ), hypertrophic stimuli including PE, Ang II, and ET-1 activate NHE-1 via stimulation of their respective receptors (R). NHE-1 activation and the resultant increase

    Article Snippet: The expression levels of ANP, α-skeletal actin, NHE-1, MCIP, and 18S rRNA genes were determined in 10 µL reaction volume using SYBR Green Jumpstart Taq ReadyMix DNA polymerase (Sigma-Aldrich), and fluorescence was measured and quantified using a DNA Engine Opticon 2 System (MJ Research, Waltham, MA, USA).

    Techniques: Activation Assay