nhei Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    New England Biolabs nhei
    Electrophoresis of pPrime- fliC Recombinant Plasmids From White Colonies on 1% Agarose Gel Lane 1 to 6: extracted Pprime- fliC plasmids; Lane 8 to 13: Pprime- fliC plasmids digested by <t>XhoI;</t> Lane 15 to 20: Pprime- fliC plasmids digested by XhoI and <t>NheI;</t> Lane 7 and 14: 1 Kbp DNA ladder vivantis.
    Nhei, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 2637 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nhei/product/New England Biolabs
    Average 99 stars, based on 2637 article reviews
    Price from $9.99 to $1999.99
    nhei - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher nhei
    Digestion of 746-bp 23S rRNA obtained by <t>PCR</t> with <t>NheI</t> and separated on 2% agarose gel in 1× Tris-acetate-EDTA buffer. Lane 1, φX174 replicative-form DNA/HaeIII molecular mass standard; lanes 2 and 4, linezolid-susceptible strains SM888 and SM913 (MIC, 2 μg/ml); lanes 3 and 5, strains SM912 and SM925 (MIC, 8 μg/ml); lanes 6 and 7, strains SM943 and SM944 (MIC, 64 μg/ml).
    Nhei, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2388 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nhei/product/Thermo Fisher
    Average 99 stars, based on 2388 article reviews
    Price from $9.99 to $1999.99
    nhei - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Promega nhei
    Digestion of 746-bp 23S rRNA obtained by <t>PCR</t> with <t>NheI</t> and separated on 2% agarose gel in 1× Tris-acetate-EDTA buffer. Lane 1, φX174 replicative-form DNA/HaeIII molecular mass standard; lanes 2 and 4, linezolid-susceptible strains SM888 and SM913 (MIC, 2 μg/ml); lanes 3 and 5, strains SM912 and SM925 (MIC, 8 μg/ml); lanes 6 and 7, strains SM943 and SM944 (MIC, 64 μg/ml).
    Nhei, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 1753 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nhei/product/Promega
    Average 99 stars, based on 1753 article reviews
    Price from $9.99 to $1999.99
    nhei - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    New England Biolabs nhei hf
    Digestion of 746-bp 23S rRNA obtained by <t>PCR</t> with <t>NheI</t> and separated on 2% agarose gel in 1× Tris-acetate-EDTA buffer. Lane 1, φX174 replicative-form DNA/HaeIII molecular mass standard; lanes 2 and 4, linezolid-susceptible strains SM888 and SM913 (MIC, 2 μg/ml); lanes 3 and 5, strains SM912 and SM925 (MIC, 8 μg/ml); lanes 6 and 7, strains SM943 and SM944 (MIC, 64 μg/ml).
    Nhei Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 435 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nhei hf/product/New England Biolabs
    Average 99 stars, based on 435 article reviews
    Price from $9.99 to $1999.99
    nhei hf - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher fastdigest nhei
    Digestion of 746-bp 23S rRNA obtained by <t>PCR</t> with <t>NheI</t> and separated on 2% agarose gel in 1× Tris-acetate-EDTA buffer. Lane 1, φX174 replicative-form DNA/HaeIII molecular mass standard; lanes 2 and 4, linezolid-susceptible strains SM888 and SM913 (MIC, 2 μg/ml); lanes 3 and 5, strains SM912 and SM925 (MIC, 8 μg/ml); lanes 6 and 7, strains SM943 and SM944 (MIC, 64 μg/ml).
    Fastdigest Nhei, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fastdigest nhei/product/Thermo Fisher
    Average 99 stars, based on 41 article reviews
    Price from $9.99 to $1999.99
    fastdigest nhei - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    97
    Jena Bioscience nhei restriction enzymes
    Digestion by <t>NheI</t> and <t>SalI</t> restriction enzymes, (A) pLexyhyg2; lane1, digestion by NheI; lane2, digestion of stuffer fragment by NheI and SalI (1000bp); lane3, DNA size marker 100–100000 bp (Fermentas, Lithuania). (B) PCR product digestion; lane1 PCR product 717bp.; lane2 digestion by NheI (618 bp); lane3 digesestion by NheI and SalI 531bp. fragment; lane4, DNA size marker 100–3000 bp (Fermentas, Lithuania)
    Nhei Restriction Enzymes, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 97/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nhei restriction enzymes/product/Jena Bioscience
    Average 97 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    nhei restriction enzymes - by Bioz Stars, 2020-04
    97/100 stars
      Buy from Supplier

    99
    Millipore nhei
    Linear view of consensus optical maps with <t>DNA</t> sequence-based maps. Solid black arrows represent the locations of missing cuts in the consensus optical maps. The XbaI and <t>NheI</t> consensus optical maps extend to the left of the origin of the DNA sequence-based map because both optical maps had a missing cut at that position.
    Nhei, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 975 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nhei/product/Millipore
    Average 99 stars, based on 975 article reviews
    Price from $9.99 to $1999.99
    nhei - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    nhei  (Roche)
    94
    Roche nhei
    ChIP analysis confirms binding of hAR to 5′ UTR ARE . a Line diagram (not to scale) of the hAR 5′ UTR showing the recognition sites of the restriction endonucleases <t>NheI</t> and <t>PvuII</t> used to digest chromatin and plasmid, plus the forward (F) and reverse (R) primers ( solid arrows ) used for ChIP semiquantitative PCR. Oligonucleotides Gen-R and Vect-R are specific for the genomic and plasmid vector sequences, respectively, and the bent arrow indicates the transcriptional start site. b , c and d Representative agarose gels of PCR amplified immunoprecipitated DNA. b LNCaP cells were treated with either vehicle or 10 nM DHT (shown above gel) and ChIP was performed using PG21 anti-hAR antibody. Precipitated genomic DNA was amplified using primers for the ARE in the hAR 5′ UTR ( ARE ), or in the PSA upstream promoter ( PSA-ARE-III ) with DHT treated cells. Lanes : IP input sample, Ig preimmune rabbit IgG, Ab antibody. Charts display values expressed as percentage of input DNA and represent means ± S.D, * p
    Nhei, supplied by Roche, used in various techniques. Bioz Stars score: 94/100, based on 105 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nhei/product/Roche
    Average 94 stars, based on 105 article reviews
    Price from $9.99 to $1999.99
    nhei - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    nhei  (Toyobo)
    81
    Toyobo nhei
    ChIP analysis confirms binding of hAR to 5′ UTR ARE . a Line diagram (not to scale) of the hAR 5′ UTR showing the recognition sites of the restriction endonucleases <t>NheI</t> and <t>PvuII</t> used to digest chromatin and plasmid, plus the forward (F) and reverse (R) primers ( solid arrows ) used for ChIP semiquantitative PCR. Oligonucleotides Gen-R and Vect-R are specific for the genomic and plasmid vector sequences, respectively, and the bent arrow indicates the transcriptional start site. b , c and d Representative agarose gels of PCR amplified immunoprecipitated DNA. b LNCaP cells were treated with either vehicle or 10 nM DHT (shown above gel) and ChIP was performed using PG21 anti-hAR antibody. Precipitated genomic DNA was amplified using primers for the ARE in the hAR 5′ UTR ( ARE ), or in the PSA upstream promoter ( PSA-ARE-III ) with DHT treated cells. Lanes : IP input sample, Ig preimmune rabbit IgG, Ab antibody. Charts display values expressed as percentage of input DNA and represent means ± S.D, * p
    Nhei, supplied by Toyobo, used in various techniques. Bioz Stars score: 81/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nhei/product/Toyobo
    Average 81 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    nhei - by Bioz Stars, 2020-04
    81/100 stars
      Buy from Supplier

    97
    Addgene inc nhei
    ChIP analysis confirms binding of hAR to 5′ UTR ARE . a Line diagram (not to scale) of the hAR 5′ UTR showing the recognition sites of the restriction endonucleases <t>NheI</t> and <t>PvuII</t> used to digest chromatin and plasmid, plus the forward (F) and reverse (R) primers ( solid arrows ) used for ChIP semiquantitative PCR. Oligonucleotides Gen-R and Vect-R are specific for the genomic and plasmid vector sequences, respectively, and the bent arrow indicates the transcriptional start site. b , c and d Representative agarose gels of PCR amplified immunoprecipitated DNA. b LNCaP cells were treated with either vehicle or 10 nM DHT (shown above gel) and ChIP was performed using PG21 anti-hAR antibody. Precipitated genomic DNA was amplified using primers for the ARE in the hAR 5′ UTR ( ARE ), or in the PSA upstream promoter ( PSA-ARE-III ) with DHT treated cells. Lanes : IP input sample, Ig preimmune rabbit IgG, Ab antibody. Charts display values expressed as percentage of input DNA and represent means ± S.D, * p
    Nhei, supplied by Addgene inc, used in various techniques. Bioz Stars score: 97/100, based on 437 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nhei/product/Addgene inc
    Average 97 stars, based on 437 article reviews
    Price from $9.99 to $1999.99
    nhei - by Bioz Stars, 2020-04
    97/100 stars
      Buy from Supplier

    93
    Roche nhei asp718
    ChIP analysis confirms binding of hAR to 5′ UTR ARE . a Line diagram (not to scale) of the hAR 5′ UTR showing the recognition sites of the restriction endonucleases <t>NheI</t> and <t>PvuII</t> used to digest chromatin and plasmid, plus the forward (F) and reverse (R) primers ( solid arrows ) used for ChIP semiquantitative PCR. Oligonucleotides Gen-R and Vect-R are specific for the genomic and plasmid vector sequences, respectively, and the bent arrow indicates the transcriptional start site. b , c and d Representative agarose gels of PCR amplified immunoprecipitated DNA. b LNCaP cells were treated with either vehicle or 10 nM DHT (shown above gel) and ChIP was performed using PG21 anti-hAR antibody. Precipitated genomic DNA was amplified using primers for the ARE in the hAR 5′ UTR ( ARE ), or in the PSA upstream promoter ( PSA-ARE-III ) with DHT treated cells. Lanes : IP input sample, Ig preimmune rabbit IgG, Ab antibody. Charts display values expressed as percentage of input DNA and represent means ± S.D, * p
    Nhei Asp718, supplied by Roche, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nhei asp718/product/Roche
    Average 93 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    nhei asp718 - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    89
    GenScript nhei noti
    ChIP analysis confirms binding of hAR to 5′ UTR ARE . a Line diagram (not to scale) of the hAR 5′ UTR showing the recognition sites of the restriction endonucleases <t>NheI</t> and <t>PvuII</t> used to digest chromatin and plasmid, plus the forward (F) and reverse (R) primers ( solid arrows ) used for ChIP semiquantitative PCR. Oligonucleotides Gen-R and Vect-R are specific for the genomic and plasmid vector sequences, respectively, and the bent arrow indicates the transcriptional start site. b , c and d Representative agarose gels of PCR amplified immunoprecipitated DNA. b LNCaP cells were treated with either vehicle or 10 nM DHT (shown above gel) and ChIP was performed using PG21 anti-hAR antibody. Precipitated genomic DNA was amplified using primers for the ARE in the hAR 5′ UTR ( ARE ), or in the PSA upstream promoter ( PSA-ARE-III ) with DHT treated cells. Lanes : IP input sample, Ig preimmune rabbit IgG, Ab antibody. Charts display values expressed as percentage of input DNA and represent means ± S.D, * p
    Nhei Noti, supplied by GenScript, used in various techniques. Bioz Stars score: 89/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nhei noti/product/GenScript
    Average 89 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    nhei noti - by Bioz Stars, 2020-04
    89/100 stars
      Buy from Supplier

    96
    Promega pvuii nhei
    ChIP analysis confirms binding of hAR to 5′ UTR ARE . a Line diagram (not to scale) of the hAR 5′ UTR showing the recognition sites of the restriction endonucleases <t>NheI</t> and <t>PvuII</t> used to digest chromatin and plasmid, plus the forward (F) and reverse (R) primers ( solid arrows ) used for ChIP semiquantitative PCR. Oligonucleotides Gen-R and Vect-R are specific for the genomic and plasmid vector sequences, respectively, and the bent arrow indicates the transcriptional start site. b , c and d Representative agarose gels of PCR amplified immunoprecipitated DNA. b LNCaP cells were treated with either vehicle or 10 nM DHT (shown above gel) and ChIP was performed using PG21 anti-hAR antibody. Precipitated genomic DNA was amplified using primers for the ARE in the hAR 5′ UTR ( ARE ), or in the PSA upstream promoter ( PSA-ARE-III ) with DHT treated cells. Lanes : IP input sample, Ig preimmune rabbit IgG, Ab antibody. Charts display values expressed as percentage of input DNA and represent means ± S.D, * p
    Pvuii Nhei, supplied by Promega, used in various techniques. Bioz Stars score: 96/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pvuii nhei/product/Promega
    Average 96 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    pvuii nhei - by Bioz Stars, 2020-04
    96/100 stars
      Buy from Supplier

    84
    Roche bgli nhei
    ChIP analysis confirms binding of hAR to 5′ UTR ARE . a Line diagram (not to scale) of the hAR 5′ UTR showing the recognition sites of the restriction endonucleases <t>NheI</t> and <t>PvuII</t> used to digest chromatin and plasmid, plus the forward (F) and reverse (R) primers ( solid arrows ) used for ChIP semiquantitative PCR. Oligonucleotides Gen-R and Vect-R are specific for the genomic and plasmid vector sequences, respectively, and the bent arrow indicates the transcriptional start site. b , c and d Representative agarose gels of PCR amplified immunoprecipitated DNA. b LNCaP cells were treated with either vehicle or 10 nM DHT (shown above gel) and ChIP was performed using PG21 anti-hAR antibody. Precipitated genomic DNA was amplified using primers for the ARE in the hAR 5′ UTR ( ARE ), or in the PSA upstream promoter ( PSA-ARE-III ) with DHT treated cells. Lanes : IP input sample, Ig preimmune rabbit IgG, Ab antibody. Charts display values expressed as percentage of input DNA and represent means ± S.D, * p
    Bgli Nhei, supplied by Roche, used in various techniques. Bioz Stars score: 84/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bgli nhei/product/Roche
    Average 84 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    bgli nhei - by Bioz Stars, 2020-04
    84/100 stars
      Buy from Supplier

    87
    Thermo Fisher nhei ecori
    ChIP analysis confirms binding of hAR to 5′ UTR ARE . a Line diagram (not to scale) of the hAR 5′ UTR showing the recognition sites of the restriction endonucleases <t>NheI</t> and <t>PvuII</t> used to digest chromatin and plasmid, plus the forward (F) and reverse (R) primers ( solid arrows ) used for ChIP semiquantitative PCR. Oligonucleotides Gen-R and Vect-R are specific for the genomic and plasmid vector sequences, respectively, and the bent arrow indicates the transcriptional start site. b , c and d Representative agarose gels of PCR amplified immunoprecipitated DNA. b LNCaP cells were treated with either vehicle or 10 nM DHT (shown above gel) and ChIP was performed using PG21 anti-hAR antibody. Precipitated genomic DNA was amplified using primers for the ARE in the hAR 5′ UTR ( ARE ), or in the PSA upstream promoter ( PSA-ARE-III ) with DHT treated cells. Lanes : IP input sample, Ig preimmune rabbit IgG, Ab antibody. Charts display values expressed as percentage of input DNA and represent means ± S.D, * p
    Nhei Ecori, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nhei ecori/product/Thermo Fisher
    Average 87 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    nhei ecori - by Bioz Stars, 2020-04
    87/100 stars
      Buy from Supplier

    95
    TaKaRa nhei xhoi
    ChIP analysis confirms binding of hAR to 5′ UTR ARE . a Line diagram (not to scale) of the hAR 5′ UTR showing the recognition sites of the restriction endonucleases <t>NheI</t> and <t>PvuII</t> used to digest chromatin and plasmid, plus the forward (F) and reverse (R) primers ( solid arrows ) used for ChIP semiquantitative PCR. Oligonucleotides Gen-R and Vect-R are specific for the genomic and plasmid vector sequences, respectively, and the bent arrow indicates the transcriptional start site. b , c and d Representative agarose gels of PCR amplified immunoprecipitated DNA. b LNCaP cells were treated with either vehicle or 10 nM DHT (shown above gel) and ChIP was performed using PG21 anti-hAR antibody. Precipitated genomic DNA was amplified using primers for the ARE in the hAR 5′ UTR ( ARE ), or in the PSA upstream promoter ( PSA-ARE-III ) with DHT treated cells. Lanes : IP input sample, Ig preimmune rabbit IgG, Ab antibody. Charts display values expressed as percentage of input DNA and represent means ± S.D, * p
    Nhei Xhoi, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nhei xhoi/product/TaKaRa
    Average 95 stars, based on 30 article reviews
    Price from $9.99 to $1999.99
    nhei xhoi - by Bioz Stars, 2020-04
    95/100 stars
      Buy from Supplier

    97
    Roche kpni nhei
    ChIP analysis confirms binding of hAR to 5′ UTR ARE . a Line diagram (not to scale) of the hAR 5′ UTR showing the recognition sites of the restriction endonucleases <t>NheI</t> and <t>PvuII</t> used to digest chromatin and plasmid, plus the forward (F) and reverse (R) primers ( solid arrows ) used for ChIP semiquantitative PCR. Oligonucleotides Gen-R and Vect-R are specific for the genomic and plasmid vector sequences, respectively, and the bent arrow indicates the transcriptional start site. b , c and d Representative agarose gels of PCR amplified immunoprecipitated DNA. b LNCaP cells were treated with either vehicle or 10 nM DHT (shown above gel) and ChIP was performed using PG21 anti-hAR antibody. Precipitated genomic DNA was amplified using primers for the ARE in the hAR 5′ UTR ( ARE ), or in the PSA upstream promoter ( PSA-ARE-III ) with DHT treated cells. Lanes : IP input sample, Ig preimmune rabbit IgG, Ab antibody. Charts display values expressed as percentage of input DNA and represent means ± S.D, * p
    Kpni Nhei, supplied by Roche, used in various techniques. Bioz Stars score: 97/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kpni nhei/product/Roche
    Average 97 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    kpni nhei - by Bioz Stars, 2020-04
    97/100 stars
      Buy from Supplier

    86
    Promega mlui nhei
    ChIP analysis confirms binding of hAR to 5′ UTR ARE . a Line diagram (not to scale) of the hAR 5′ UTR showing the recognition sites of the restriction endonucleases <t>NheI</t> and <t>PvuII</t> used to digest chromatin and plasmid, plus the forward (F) and reverse (R) primers ( solid arrows ) used for ChIP semiquantitative PCR. Oligonucleotides Gen-R and Vect-R are specific for the genomic and plasmid vector sequences, respectively, and the bent arrow indicates the transcriptional start site. b , c and d Representative agarose gels of PCR amplified immunoprecipitated DNA. b LNCaP cells were treated with either vehicle or 10 nM DHT (shown above gel) and ChIP was performed using PG21 anti-hAR antibody. Precipitated genomic DNA was amplified using primers for the ARE in the hAR 5′ UTR ( ARE ), or in the PSA upstream promoter ( PSA-ARE-III ) with DHT treated cells. Lanes : IP input sample, Ig preimmune rabbit IgG, Ab antibody. Charts display values expressed as percentage of input DNA and represent means ± S.D, * p
    Mlui Nhei, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mlui nhei/product/Promega
    Average 86 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    mlui nhei - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

    79
    Thermo Fisher xbai nhei
    ChIP analysis confirms binding of hAR to 5′ UTR ARE . a Line diagram (not to scale) of the hAR 5′ UTR showing the recognition sites of the restriction endonucleases <t>NheI</t> and <t>PvuII</t> used to digest chromatin and plasmid, plus the forward (F) and reverse (R) primers ( solid arrows ) used for ChIP semiquantitative PCR. Oligonucleotides Gen-R and Vect-R are specific for the genomic and plasmid vector sequences, respectively, and the bent arrow indicates the transcriptional start site. b , c and d Representative agarose gels of PCR amplified immunoprecipitated DNA. b LNCaP cells were treated with either vehicle or 10 nM DHT (shown above gel) and ChIP was performed using PG21 anti-hAR antibody. Precipitated genomic DNA was amplified using primers for the ARE in the hAR 5′ UTR ( ARE ), or in the PSA upstream promoter ( PSA-ARE-III ) with DHT treated cells. Lanes : IP input sample, Ig preimmune rabbit IgG, Ab antibody. Charts display values expressed as percentage of input DNA and represent means ± S.D, * p
    Xbai Nhei, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xbai nhei/product/Thermo Fisher
    Average 79 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    xbai nhei - by Bioz Stars, 2020-04
    79/100 stars
      Buy from Supplier

    85
    Roche stui nhei
    ChIP analysis confirms binding of hAR to 5′ UTR ARE . a Line diagram (not to scale) of the hAR 5′ UTR showing the recognition sites of the restriction endonucleases <t>NheI</t> and <t>PvuII</t> used to digest chromatin and plasmid, plus the forward (F) and reverse (R) primers ( solid arrows ) used for ChIP semiquantitative PCR. Oligonucleotides Gen-R and Vect-R are specific for the genomic and plasmid vector sequences, respectively, and the bent arrow indicates the transcriptional start site. b , c and d Representative agarose gels of PCR amplified immunoprecipitated DNA. b LNCaP cells were treated with either vehicle or 10 nM DHT (shown above gel) and ChIP was performed using PG21 anti-hAR antibody. Precipitated genomic DNA was amplified using primers for the ARE in the hAR 5′ UTR ( ARE ), or in the PSA upstream promoter ( PSA-ARE-III ) with DHT treated cells. Lanes : IP input sample, Ig preimmune rabbit IgG, Ab antibody. Charts display values expressed as percentage of input DNA and represent means ± S.D, * p
    Stui Nhei, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/stui nhei/product/Roche
    Average 85 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    stui nhei - by Bioz Stars, 2020-04
    85/100 stars
      Buy from Supplier

    84
    GenScript nsii nhei
    ChIP analysis confirms binding of hAR to 5′ UTR ARE . a Line diagram (not to scale) of the hAR 5′ UTR showing the recognition sites of the restriction endonucleases <t>NheI</t> and <t>PvuII</t> used to digest chromatin and plasmid, plus the forward (F) and reverse (R) primers ( solid arrows ) used for ChIP semiquantitative PCR. Oligonucleotides Gen-R and Vect-R are specific for the genomic and plasmid vector sequences, respectively, and the bent arrow indicates the transcriptional start site. b , c and d Representative agarose gels of PCR amplified immunoprecipitated DNA. b LNCaP cells were treated with either vehicle or 10 nM DHT (shown above gel) and ChIP was performed using PG21 anti-hAR antibody. Precipitated genomic DNA was amplified using primers for the ARE in the hAR 5′ UTR ( ARE ), or in the PSA upstream promoter ( PSA-ARE-III ) with DHT treated cells. Lanes : IP input sample, Ig preimmune rabbit IgG, Ab antibody. Charts display values expressed as percentage of input DNA and represent means ± S.D, * p
    Nsii Nhei, supplied by GenScript, used in various techniques. Bioz Stars score: 84/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nsii nhei/product/GenScript
    Average 84 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    nsii nhei - by Bioz Stars, 2020-04
    84/100 stars
      Buy from Supplier

    88
    TaKaRa noti nhei
    ChIP analysis confirms binding of hAR to 5′ UTR ARE . a Line diagram (not to scale) of the hAR 5′ UTR showing the recognition sites of the restriction endonucleases <t>NheI</t> and <t>PvuII</t> used to digest chromatin and plasmid, plus the forward (F) and reverse (R) primers ( solid arrows ) used for ChIP semiquantitative PCR. Oligonucleotides Gen-R and Vect-R are specific for the genomic and plasmid vector sequences, respectively, and the bent arrow indicates the transcriptional start site. b , c and d Representative agarose gels of PCR amplified immunoprecipitated DNA. b LNCaP cells were treated with either vehicle or 10 nM DHT (shown above gel) and ChIP was performed using PG21 anti-hAR antibody. Precipitated genomic DNA was amplified using primers for the ARE in the hAR 5′ UTR ( ARE ), or in the PSA upstream promoter ( PSA-ARE-III ) with DHT treated cells. Lanes : IP input sample, Ig preimmune rabbit IgG, Ab antibody. Charts display values expressed as percentage of input DNA and represent means ± S.D, * p
    Noti Nhei, supplied by TaKaRa, used in various techniques. Bioz Stars score: 88/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/noti nhei/product/TaKaRa
    Average 88 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    noti nhei - by Bioz Stars, 2020-04
    88/100 stars
      Buy from Supplier

    87
    TaKaRa nhei nhei fragment
    ChIP analysis confirms binding of hAR to 5′ UTR ARE . a Line diagram (not to scale) of the hAR 5′ UTR showing the recognition sites of the restriction endonucleases <t>NheI</t> and <t>PvuII</t> used to digest chromatin and plasmid, plus the forward (F) and reverse (R) primers ( solid arrows ) used for ChIP semiquantitative PCR. Oligonucleotides Gen-R and Vect-R are specific for the genomic and plasmid vector sequences, respectively, and the bent arrow indicates the transcriptional start site. b , c and d Representative agarose gels of PCR amplified immunoprecipitated DNA. b LNCaP cells were treated with either vehicle or 10 nM DHT (shown above gel) and ChIP was performed using PG21 anti-hAR antibody. Precipitated genomic DNA was amplified using primers for the ARE in the hAR 5′ UTR ( ARE ), or in the PSA upstream promoter ( PSA-ARE-III ) with DHT treated cells. Lanes : IP input sample, Ig preimmune rabbit IgG, Ab antibody. Charts display values expressed as percentage of input DNA and represent means ± S.D, * p
    Nhei Nhei Fragment, supplied by TaKaRa, used in various techniques. Bioz Stars score: 87/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nhei nhei fragment/product/TaKaRa
    Average 87 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    nhei nhei fragment - by Bioz Stars, 2020-04
    87/100 stars
      Buy from Supplier

    97
    TaKaRa nhei linearized pegfpc3
    ChIP analysis confirms binding of hAR to 5′ UTR ARE . a Line diagram (not to scale) of the hAR 5′ UTR showing the recognition sites of the restriction endonucleases <t>NheI</t> and <t>PvuII</t> used to digest chromatin and plasmid, plus the forward (F) and reverse (R) primers ( solid arrows ) used for ChIP semiquantitative PCR. Oligonucleotides Gen-R and Vect-R are specific for the genomic and plasmid vector sequences, respectively, and the bent arrow indicates the transcriptional start site. b , c and d Representative agarose gels of PCR amplified immunoprecipitated DNA. b LNCaP cells were treated with either vehicle or 10 nM DHT (shown above gel) and ChIP was performed using PG21 anti-hAR antibody. Precipitated genomic DNA was amplified using primers for the ARE in the hAR 5′ UTR ( ARE ), or in the PSA upstream promoter ( PSA-ARE-III ) with DHT treated cells. Lanes : IP input sample, Ig preimmune rabbit IgG, Ab antibody. Charts display values expressed as percentage of input DNA and represent means ± S.D, * p
    Nhei Linearized Pegfpc3, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nhei linearized pegfpc3/product/TaKaRa
    Average 97 stars, based on 21 article reviews
    Price from $9.99 to $1999.99
    nhei linearized pegfpc3 - by Bioz Stars, 2020-04
    97/100 stars
      Buy from Supplier

    94
    GenScript nhei restriction sites
    ChIP analysis confirms binding of hAR to 5′ UTR ARE . a Line diagram (not to scale) of the hAR 5′ UTR showing the recognition sites of the restriction endonucleases <t>NheI</t> and <t>PvuII</t> used to digest chromatin and plasmid, plus the forward (F) and reverse (R) primers ( solid arrows ) used for ChIP semiquantitative PCR. Oligonucleotides Gen-R and Vect-R are specific for the genomic and plasmid vector sequences, respectively, and the bent arrow indicates the transcriptional start site. b , c and d Representative agarose gels of PCR amplified immunoprecipitated DNA. b LNCaP cells were treated with either vehicle or 10 nM DHT (shown above gel) and ChIP was performed using PG21 anti-hAR antibody. Precipitated genomic DNA was amplified using primers for the ARE in the hAR 5′ UTR ( ARE ), or in the PSA upstream promoter ( PSA-ARE-III ) with DHT treated cells. Lanes : IP input sample, Ig preimmune rabbit IgG, Ab antibody. Charts display values expressed as percentage of input DNA and represent means ± S.D, * p
    Nhei Restriction Sites, supplied by GenScript, used in various techniques. Bioz Stars score: 94/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nhei restriction sites/product/GenScript
    Average 94 stars, based on 22 article reviews
    Price from $9.99 to $1999.99
    nhei restriction sites - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    93
    Promega nhei xhoi sites
    ChIP analysis confirms binding of hAR to 5′ UTR ARE . a Line diagram (not to scale) of the hAR 5′ UTR showing the recognition sites of the restriction endonucleases <t>NheI</t> and <t>PvuII</t> used to digest chromatin and plasmid, plus the forward (F) and reverse (R) primers ( solid arrows ) used for ChIP semiquantitative PCR. Oligonucleotides Gen-R and Vect-R are specific for the genomic and plasmid vector sequences, respectively, and the bent arrow indicates the transcriptional start site. b , c and d Representative agarose gels of PCR amplified immunoprecipitated DNA. b LNCaP cells were treated with either vehicle or 10 nM DHT (shown above gel) and ChIP was performed using PG21 anti-hAR antibody. Precipitated genomic DNA was amplified using primers for the ARE in the hAR 5′ UTR ( ARE ), or in the PSA upstream promoter ( PSA-ARE-III ) with DHT treated cells. Lanes : IP input sample, Ig preimmune rabbit IgG, Ab antibody. Charts display values expressed as percentage of input DNA and represent means ± S.D, * p
    Nhei Xhoi Sites, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nhei xhoi sites/product/Promega
    Average 93 stars, based on 63 article reviews
    Price from $9.99 to $1999.99
    nhei xhoi sites - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    79
    TaKaRa nhei ecorv sites
    ChIP analysis confirms binding of hAR to 5′ UTR ARE . a Line diagram (not to scale) of the hAR 5′ UTR showing the recognition sites of the restriction endonucleases <t>NheI</t> and <t>PvuII</t> used to digest chromatin and plasmid, plus the forward (F) and reverse (R) primers ( solid arrows ) used for ChIP semiquantitative PCR. Oligonucleotides Gen-R and Vect-R are specific for the genomic and plasmid vector sequences, respectively, and the bent arrow indicates the transcriptional start site. b , c and d Representative agarose gels of PCR amplified immunoprecipitated DNA. b LNCaP cells were treated with either vehicle or 10 nM DHT (shown above gel) and ChIP was performed using PG21 anti-hAR antibody. Precipitated genomic DNA was amplified using primers for the ARE in the hAR 5′ UTR ( ARE ), or in the PSA upstream promoter ( PSA-ARE-III ) with DHT treated cells. Lanes : IP input sample, Ig preimmune rabbit IgG, Ab antibody. Charts display values expressed as percentage of input DNA and represent means ± S.D, * p
    Nhei Ecorv Sites, supplied by TaKaRa, used in various techniques. Bioz Stars score: 79/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nhei ecorv sites/product/TaKaRa
    Average 79 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    nhei ecorv sites - by Bioz Stars, 2020-04
    79/100 stars
      Buy from Supplier

    80
    Promega nhei bglii digested
    ChIP analysis confirms binding of hAR to 5′ UTR ARE . a Line diagram (not to scale) of the hAR 5′ UTR showing the recognition sites of the restriction endonucleases <t>NheI</t> and <t>PvuII</t> used to digest chromatin and plasmid, plus the forward (F) and reverse (R) primers ( solid arrows ) used for ChIP semiquantitative PCR. Oligonucleotides Gen-R and Vect-R are specific for the genomic and plasmid vector sequences, respectively, and the bent arrow indicates the transcriptional start site. b , c and d Representative agarose gels of PCR amplified immunoprecipitated DNA. b LNCaP cells were treated with either vehicle or 10 nM DHT (shown above gel) and ChIP was performed using PG21 anti-hAR antibody. Precipitated genomic DNA was amplified using primers for the ARE in the hAR 5′ UTR ( ARE ), or in the PSA upstream promoter ( PSA-ARE-III ) with DHT treated cells. Lanes : IP input sample, Ig preimmune rabbit IgG, Ab antibody. Charts display values expressed as percentage of input DNA and represent means ± S.D, * p
    Nhei Bglii Digested, supplied by Promega, used in various techniques. Bioz Stars score: 80/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nhei bglii digested/product/Promega
    Average 80 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    nhei bglii digested - by Bioz Stars, 2020-04
    80/100 stars
      Buy from Supplier

    Image Search Results


    Electrophoresis of pPrime- fliC Recombinant Plasmids From White Colonies on 1% Agarose Gel Lane 1 to 6: extracted Pprime- fliC plasmids; Lane 8 to 13: Pprime- fliC plasmids digested by XhoI; Lane 15 to 20: Pprime- fliC plasmids digested by XhoI and NheI; Lane 7 and 14: 1 Kbp DNA ladder vivantis.

    Journal: Jundishapur Journal of Microbiology

    Article Title: Cloning of fliC Gene From Salmonella typhimurium in the Expression Vector pVAX1 and Evaluation of its Expression in Eukaryotic Cells

    doi: 10.5812/jjm.12351

    Figure Lengend Snippet: Electrophoresis of pPrime- fliC Recombinant Plasmids From White Colonies on 1% Agarose Gel Lane 1 to 6: extracted Pprime- fliC plasmids; Lane 8 to 13: Pprime- fliC plasmids digested by XhoI; Lane 15 to 20: Pprime- fliC plasmids digested by XhoI and NheI; Lane 7 and 14: 1 Kbp DNA ladder vivantis.

    Article Snippet: The recombinant plasmids were detected by restriction digestion with NheI and XhoI enzymes (New England BioLabs, USA) and confirmed by sequence analysis (Bioneer, Korea).

    Techniques: Electrophoresis, Recombinant, Agarose Gel Electrophoresis

    AgaroseGel Electrophoresis of Restriction Enzyme Digestion of Purified pVAX- fliC Recombinant Plasmid Lane 1: pVAX- fliC plasmid digested by XhoI had one band (4419 bp); Lane 2: Double Digestion by NheI and XhoI on pVAX- fliC plasmid had two bands that were 1509 bp (down) and 2910 bp (up); Lane 3: 1 Kbp DNA ladder Vivantis.

    Journal: Jundishapur Journal of Microbiology

    Article Title: Cloning of fliC Gene From Salmonella typhimurium in the Expression Vector pVAX1 and Evaluation of its Expression in Eukaryotic Cells

    doi: 10.5812/jjm.12351

    Figure Lengend Snippet: AgaroseGel Electrophoresis of Restriction Enzyme Digestion of Purified pVAX- fliC Recombinant Plasmid Lane 1: pVAX- fliC plasmid digested by XhoI had one band (4419 bp); Lane 2: Double Digestion by NheI and XhoI on pVAX- fliC plasmid had two bands that were 1509 bp (down) and 2910 bp (up); Lane 3: 1 Kbp DNA ladder Vivantis.

    Article Snippet: The recombinant plasmids were detected by restriction digestion with NheI and XhoI enzymes (New England BioLabs, USA) and confirmed by sequence analysis (Bioneer, Korea).

    Techniques: Electrophoresis, Purification, Recombinant, Plasmid Preparation

    Restriction maps of plasmid and bacteriophage DNA obtained from V. parahaemolyticus strain PMC 58.5. N, H, and S correspond to NheI, HindIII, and SphI restriction enzymes sites, respectively. A hypothetical circular map is shown in the middle, with the possible ends shown with a red arrow and the restriction sites with a black line. b, bacteriophage; p, plasmid.

    Journal: Applied and Environmental Microbiology

    Article Title: Enhancement of UV Light Sensitivity of a Vibrio parahaemolyticus O3:K6 Pandemic Strain Due to Natural Lysogenization by a Telomeric Phage ▿

    doi: 10.1128/AEM.01995-08

    Figure Lengend Snippet: Restriction maps of plasmid and bacteriophage DNA obtained from V. parahaemolyticus strain PMC 58.5. N, H, and S correspond to NheI, HindIII, and SphI restriction enzymes sites, respectively. A hypothetical circular map is shown in the middle, with the possible ends shown with a red arrow and the restriction sites with a black line. b, bacteriophage; p, plasmid.

    Article Snippet: Restriction site mapping was performed by digestion with restriction enzymes NheI, HindIII, and SphI from New England Biolabs (Massachusetts), according to the manufacturer's instructions.

    Techniques: Plasmid Preparation

    Dendrogram based on Nhe I PFGE patterns of 40 N. gonorrhoeae isolates.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Molecular Basis of High-Level Ciprofloxacin Resistance in Neisseria gonorrhoeae Strains Isolated in Denmark from 1995 to 1998

    doi: 10.1128/AAC.45.1.117-123.2001

    Figure Lengend Snippet: Dendrogram based on Nhe I PFGE patterns of 40 N. gonorrhoeae isolates.

    Article Snippet: Slices of DNA-containing agarose blocks were digested with Spe I and Nhe I (New England Biolabs) overnight at 37°C in 100 μl of restriction endonuclease buffer containing 10 U of enzyme.

    Techniques:

    Digestion of 746-bp 23S rRNA obtained by PCR with NheI and separated on 2% agarose gel in 1× Tris-acetate-EDTA buffer. Lane 1, φX174 replicative-form DNA/HaeIII molecular mass standard; lanes 2 and 4, linezolid-susceptible strains SM888 and SM913 (MIC, 2 μg/ml); lanes 3 and 5, strains SM912 and SM925 (MIC, 8 μg/ml); lanes 6 and 7, strains SM943 and SM944 (MIC, 64 μg/ml).

    Journal: Journal of Clinical Microbiology

    Article Title: Emergence of Linezolid Resistance in the Vancomycin-Resistant Enterococcus faecium Multilocus Sequence Typing C1 Epidemic Lineage

    doi: 10.1128/JCM.44.3.1153-1155.2006

    Figure Lengend Snippet: Digestion of 746-bp 23S rRNA obtained by PCR with NheI and separated on 2% agarose gel in 1× Tris-acetate-EDTA buffer. Lane 1, φX174 replicative-form DNA/HaeIII molecular mass standard; lanes 2 and 4, linezolid-susceptible strains SM888 and SM913 (MIC, 2 μg/ml); lanes 3 and 5, strains SM912 and SM925 (MIC, 8 μg/ml); lanes 6 and 7, strains SM943 and SM944 (MIC, 64 μg/ml).

    Article Snippet: The cycling conditions were as follows: an initial denaturation at 94°C for 5 min, followed by 30 cycles consisting of 94°C for 1 min, 50°C for 30 s, 72°C for 1 min, and a final extension step at 72°C for 5 min. Small amounts (5 μl) of PCR 23S rRNA were digested with 10 units of NheI (Invitrogen Life Technologies) at 37°C for 4 h and separated on 2% agarose gel in 1× Tris-acetate-EDTA buffer.

    Techniques: Polymerase Chain Reaction, Agarose Gel Electrophoresis

    Digestion by NheI and SalI restriction enzymes, (A) pLexyhyg2; lane1, digestion by NheI; lane2, digestion of stuffer fragment by NheI and SalI (1000bp); lane3, DNA size marker 100–100000 bp (Fermentas, Lithuania). (B) PCR product digestion; lane1 PCR product 717bp.; lane2 digestion by NheI (618 bp); lane3 digesestion by NheI and SalI 531bp. fragment; lane4, DNA size marker 100–3000 bp (Fermentas, Lithuania)

    Journal: Iranian Journal of Public Health

    Article Title: Expression of Recombinant Human Amelogenin in Iranian Lizard Leishmania and Its Biological Function Assay

    doi:

    Figure Lengend Snippet: Digestion by NheI and SalI restriction enzymes, (A) pLexyhyg2; lane1, digestion by NheI; lane2, digestion of stuffer fragment by NheI and SalI (1000bp); lane3, DNA size marker 100–100000 bp (Fermentas, Lithuania). (B) PCR product digestion; lane1 PCR product 717bp.; lane2 digestion by NheI (618 bp); lane3 digesestion by NheI and SalI 531bp. fragment; lane4, DNA size marker 100–3000 bp (Fermentas, Lithuania)

    Article Snippet: The synthesized gene was amplified by M13 primers ( ) and digested by using SalI and NheI restriction enzymes then sub cloned into pLEXSY-hyg2 plasmid that encoded 6His tag gene (EGE-232, Jena Bioscience, Germany).

    Techniques: Marker, Polymerase Chain Reaction

    Linear view of consensus optical maps with DNA sequence-based maps. Solid black arrows represent the locations of missing cuts in the consensus optical maps. The XbaI and NheI consensus optical maps extend to the left of the origin of the DNA sequence-based map because both optical maps had a missing cut at that position.

    Journal: Applied and Environmental Microbiology

    Article Title: Whole-Genome Shotgun Optical Mapping of Rhodospirillum rubrum

    doi: 10.1128/AEM.71.9.5511-5522.2005

    Figure Lengend Snippet: Linear view of consensus optical maps with DNA sequence-based maps. Solid black arrows represent the locations of missing cuts in the consensus optical maps. The XbaI and NheI consensus optical maps extend to the left of the origin of the DNA sequence-based map because both optical maps had a missing cut at that position.

    Article Snippet: Surface properties were assayed by digesting lambda DASH II bacteriophage DNA with 40 units of XbaI, HindIII, and NheI diluted in 200 μl of digestion buffer with 0.2% Triton X-100 (Sigma, St. Louis, MO) at 37°C to determine optimal digestion times, which ranged from 30 to 120 min.

    Techniques: Sequencing

    Use of optical maps in confirming assembly and order of sequence contigs. In silico maps of the sequence contigs were made and aligned against the whole-genome optical maps located in the center of each diagram. A) The high-resolution HindIII map enabled alignment of the small (∼80 kb) sequence contig (contig 83) between contigs 85c and 86c. Aside from the redundant alignment of contigs 87 and 90, no gaps remained in alignment between the optical map and sequence. B) The NheI map gave the clearest representation of the redundant alignment, shown in red in the optical map, of contigs 87 and 90. The alignment of contig 87 had a better match to the optical map than do the rightmost eight fragments in contig 90 (shown in the red square). C) Removing the rightmost eight fragments from contig 90 to make contigs 90a and 90b and reversing the orientation of 90b permitted alignment to the gap between contig 87 and contig 85c. Alignments were made with MapViewer Software (OpGen, Inc, Madison WI.).

    Journal: Applied and Environmental Microbiology

    Article Title: Whole-Genome Shotgun Optical Mapping of Rhodospirillum rubrum

    doi: 10.1128/AEM.71.9.5511-5522.2005

    Figure Lengend Snippet: Use of optical maps in confirming assembly and order of sequence contigs. In silico maps of the sequence contigs were made and aligned against the whole-genome optical maps located in the center of each diagram. A) The high-resolution HindIII map enabled alignment of the small (∼80 kb) sequence contig (contig 83) between contigs 85c and 86c. Aside from the redundant alignment of contigs 87 and 90, no gaps remained in alignment between the optical map and sequence. B) The NheI map gave the clearest representation of the redundant alignment, shown in red in the optical map, of contigs 87 and 90. The alignment of contig 87 had a better match to the optical map than do the rightmost eight fragments in contig 90 (shown in the red square). C) Removing the rightmost eight fragments from contig 90 to make contigs 90a and 90b and reversing the orientation of 90b permitted alignment to the gap between contig 87 and contig 85c. Alignments were made with MapViewer Software (OpGen, Inc, Madison WI.).

    Article Snippet: Surface properties were assayed by digesting lambda DASH II bacteriophage DNA with 40 units of XbaI, HindIII, and NheI diluted in 200 μl of digestion buffer with 0.2% Triton X-100 (Sigma, St. Louis, MO) at 37°C to determine optimal digestion times, which ranged from 30 to 120 min.

    Techniques: Sequencing, In Silico, Software

    Comparisons of the XbaI, NheI, and HindIII optical maps to sequence data. (A, B, and C) Plots of optical map fragment sizes versus the DNA sequence-based map fragment sizes from the finished sequence for XbaI (A), NheI (B), and HindIII (C). The error bars represent the standard deviation of optical map fragment size about the mean. (D, E, F) Plots of the relative fragment sizing error [100 × (optical map fragment size - corresponding DNA sequence-based map fragment size)/corresponding DNA sequence-based map fragment size] versus DNA sequence-based map fragment size for XbaI ( D), NheI (E), and HindIII (F).

    Journal: Applied and Environmental Microbiology

    Article Title: Whole-Genome Shotgun Optical Mapping of Rhodospirillum rubrum

    doi: 10.1128/AEM.71.9.5511-5522.2005

    Figure Lengend Snippet: Comparisons of the XbaI, NheI, and HindIII optical maps to sequence data. (A, B, and C) Plots of optical map fragment sizes versus the DNA sequence-based map fragment sizes from the finished sequence for XbaI (A), NheI (B), and HindIII (C). The error bars represent the standard deviation of optical map fragment size about the mean. (D, E, F) Plots of the relative fragment sizing error [100 × (optical map fragment size - corresponding DNA sequence-based map fragment size)/corresponding DNA sequence-based map fragment size] versus DNA sequence-based map fragment size for XbaI ( D), NheI (E), and HindIII (F).

    Article Snippet: Surface properties were assayed by digesting lambda DASH II bacteriophage DNA with 40 units of XbaI, HindIII, and NheI diluted in 200 μl of digestion buffer with 0.2% Triton X-100 (Sigma, St. Louis, MO) at 37°C to determine optimal digestion times, which ranged from 30 to 120 min.

    Techniques: Sequencing, Standard Deviation

    Cloning of HSA . a 1% agarose gel showing amplified PCR product and pET23b vector. Lane M, 1 kb DNA ladder; Lane 1, Uncut pET23b plasmid; Lane 2, a double digested pET23b vector with Nhe I and Xho I restriction enzymes; Lane 3, PCR amplified a product of HSA gene at an optimized annealing temperature of 58 °C. b 1% agarose gel showing the release of HSA gene after double digestion of constructed pETHSA vector with Nhe I and Xho I restriction enzymes. Lane M, 1 kb DNA ladder; Lane 1, Undigested pETHSA plasmid; Lane 2, digested pETHSA construct. c 12% SDS PAGE showing expression of rHSA in different strains of E. coli . Lane UI, Uninduced cells; Lane 1, Induced BL21 (DE3) E. coli cells; Lane 2, Induced Rosetta (DE3) E. coli cells; Lane 3, Induced Origami2 (DE3) E. coli cells

    Journal: Microbial Cell Factories

    Article Title: Revisiting Escherichia coli as microbial factory for enhanced production of human serum albumin

    doi: 10.1186/s12934-017-0784-8

    Figure Lengend Snippet: Cloning of HSA . a 1% agarose gel showing amplified PCR product and pET23b vector. Lane M, 1 kb DNA ladder; Lane 1, Uncut pET23b plasmid; Lane 2, a double digested pET23b vector with Nhe I and Xho I restriction enzymes; Lane 3, PCR amplified a product of HSA gene at an optimized annealing temperature of 58 °C. b 1% agarose gel showing the release of HSA gene after double digestion of constructed pETHSA vector with Nhe I and Xho I restriction enzymes. Lane M, 1 kb DNA ladder; Lane 1, Undigested pETHSA plasmid; Lane 2, digested pETHSA construct. c 12% SDS PAGE showing expression of rHSA in different strains of E. coli . Lane UI, Uninduced cells; Lane 1, Induced BL21 (DE3) E. coli cells; Lane 2, Induced Rosetta (DE3) E. coli cells; Lane 3, Induced Origami2 (DE3) E. coli cells

    Article Snippet: The PCR fragments were double-digested with Nhe I and Xho I and then subcloned into a pET23b expression vector (Novagen, USA) that had been pre-digested with the same enzymes.

    Techniques: Clone Assay, Agarose Gel Electrophoresis, Amplification, Polymerase Chain Reaction, Plasmid Preparation, Construct, SDS Page, Expressing

    ChIP analysis confirms binding of hAR to 5′ UTR ARE . a Line diagram (not to scale) of the hAR 5′ UTR showing the recognition sites of the restriction endonucleases NheI and PvuII used to digest chromatin and plasmid, plus the forward (F) and reverse (R) primers ( solid arrows ) used for ChIP semiquantitative PCR. Oligonucleotides Gen-R and Vect-R are specific for the genomic and plasmid vector sequences, respectively, and the bent arrow indicates the transcriptional start site. b , c and d Representative agarose gels of PCR amplified immunoprecipitated DNA. b LNCaP cells were treated with either vehicle or 10 nM DHT (shown above gel) and ChIP was performed using PG21 anti-hAR antibody. Precipitated genomic DNA was amplified using primers for the ARE in the hAR 5′ UTR ( ARE ), or in the PSA upstream promoter ( PSA-ARE-III ) with DHT treated cells. Lanes : IP input sample, Ig preimmune rabbit IgG, Ab antibody. Charts display values expressed as percentage of input DNA and represent means ± S.D, * p

    Journal: Hormones & Cancer

    Article Title: Negative Regulation of the Androgen Receptor Gene Through a Primate-Specific Androgen Response Element Present in the 5′ UTR

    doi: 10.1007/s12672-014-0185-y

    Figure Lengend Snippet: ChIP analysis confirms binding of hAR to 5′ UTR ARE . a Line diagram (not to scale) of the hAR 5′ UTR showing the recognition sites of the restriction endonucleases NheI and PvuII used to digest chromatin and plasmid, plus the forward (F) and reverse (R) primers ( solid arrows ) used for ChIP semiquantitative PCR. Oligonucleotides Gen-R and Vect-R are specific for the genomic and plasmid vector sequences, respectively, and the bent arrow indicates the transcriptional start site. b , c and d Representative agarose gels of PCR amplified immunoprecipitated DNA. b LNCaP cells were treated with either vehicle or 10 nM DHT (shown above gel) and ChIP was performed using PG21 anti-hAR antibody. Precipitated genomic DNA was amplified using primers for the ARE in the hAR 5′ UTR ( ARE ), or in the PSA upstream promoter ( PSA-ARE-III ) with DHT treated cells. Lanes : IP input sample, Ig preimmune rabbit IgG, Ab antibody. Charts display values expressed as percentage of input DNA and represent means ± S.D, * p

    Article Snippet: Chromatin and plasmid were digested with 400 units each PvuII (NEB) and NheI (Roche) for 15 min at 37 °C; followed by lysis and the removal of insoluble debris by centrifugation.

    Techniques: Chromatin Immunoprecipitation, Binding Assay, Plasmid Preparation, Polymerase Chain Reaction, Amplification, Immunoprecipitation