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  • 99
    New England Biolabs nhei
    Vector maps and <t>PEG</t> purification. a Basic vector maps (not to scale) for the original Tet-pLKO-Puro vector and our modified versions. b Agarose gel electrophoresis comparing DNA precipitation methods. 10 μg of EZ-Tet-pLKO vector DNA was co-digested with <t>NheI</t> + EcoRI. The digest was split into three 3 μg aliquots and precipitated with isopropanol (Iso) or polyethylene glycol (PEG) at 6 or 8% concentration. 1 μg of control DNA (uncut and cut) was run alongside 1/3 of the precipitated DNA samples
    Nhei, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 3268 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    Thermo Fisher nhei
    Digestion of 746-bp 23S rRNA obtained by <t>PCR</t> with <t>NheI</t> and separated on 2% agarose gel in 1× Tris-acetate-EDTA buffer. Lane 1, φX174 replicative-form DNA/HaeIII molecular mass standard; lanes 2 and 4, linezolid-susceptible strains SM888 and SM913 (MIC, 2 μg/ml); lanes 3 and 5, strains SM912 and SM925 (MIC, 8 μg/ml); lanes 6 and 7, strains SM943 and SM944 (MIC, 64 μg/ml).
    Nhei, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 2906 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nhei/product/Thermo Fisher
    Average 98 stars, based on 2906 article reviews
    Price from $9.99 to $1999.99
    nhei - by Bioz Stars, 2020-07
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    99
    Promega nhei
    Functional differences of the Gli1 5' UTR variants . (A) Schematic representation of the six Gli1 5' UTRs analyzed. The alternative TSSs, the individual exons and the initiator methionine codon are represented as in Figure 2A. Bold lines indicate the six 5' UTR regions, L, M, S, LΔ1B, MΔ1B, SΔ1B, which were cloned into the <t>NheI</t> site, upstream of the <t>Renilla</t> luciferase coding region (black box) of the psiCHECK2 vector as described in methods. (B, C, and D) Luciferase activity of the six reporter constructs after transfection into NIH3T3 cells, Sufu -/- MEFs, and Ptch1 -/- MEFs, respectively. Transfected NIH3T3 cells were treated with methanol (MeOH) or SAG. The Renilla luciferase activity was normalized relative to that of the Firefly luciferase. The error bars indicate the standard deviation. The statistical significance of the differences among the Gli1 5' UTR constructs is shown. (*: p
    Nhei, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 2204 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Millipore nhei
    Linear view of consensus optical maps with <t>DNA</t> sequence-based maps. Solid black arrows represent the locations of missing cuts in the consensus optical maps. The XbaI and <t>NheI</t> consensus optical maps extend to the left of the origin of the DNA sequence-based map because both optical maps had a missing cut at that position.
    Nhei, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 1145 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher fastdigest nhei
    Linear view of consensus optical maps with <t>DNA</t> sequence-based maps. Solid black arrows represent the locations of missing cuts in the consensus optical maps. The XbaI and <t>NheI</t> consensus optical maps extend to the left of the origin of the DNA sequence-based map because both optical maps had a missing cut at that position.
    Fastdigest Nhei, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 121 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nhei  (Roche)
    92
    Roche nhei
    ChIP analysis confirms binding of hAR to 5′ UTR ARE . a Line diagram (not to scale) of the hAR 5′ UTR showing the recognition sites of the restriction endonucleases <t>NheI</t> and <t>PvuII</t> used to digest chromatin and plasmid, plus the forward (F) and reverse (R) primers ( solid arrows ) used for ChIP semiquantitative PCR. Oligonucleotides Gen-R and Vect-R are specific for the genomic and plasmid vector sequences, respectively, and the bent arrow indicates the transcriptional start site. b , c and d Representative agarose gels of PCR amplified immunoprecipitated DNA. b LNCaP cells were treated with either vehicle or 10 nM DHT (shown above gel) and ChIP was performed using PG21 anti-hAR antibody. Precipitated genomic DNA was amplified using primers for the ARE in the hAR 5′ UTR ( ARE ), or in the PSA upstream promoter ( PSA-ARE-III ) with DHT treated cells. Lanes : IP input sample, Ig preimmune rabbit IgG, Ab antibody. Charts display values expressed as percentage of input DNA and represent means ± S.D, * p
    Nhei, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 129 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    New England Biolabs nhei hf
    ChIP analysis confirms binding of hAR to 5′ UTR ARE . a Line diagram (not to scale) of the hAR 5′ UTR showing the recognition sites of the restriction endonucleases <t>NheI</t> and <t>PvuII</t> used to digest chromatin and plasmid, plus the forward (F) and reverse (R) primers ( solid arrows ) used for ChIP semiquantitative PCR. Oligonucleotides Gen-R and Vect-R are specific for the genomic and plasmid vector sequences, respectively, and the bent arrow indicates the transcriptional start site. b , c and d Representative agarose gels of PCR amplified immunoprecipitated DNA. b LNCaP cells were treated with either vehicle or 10 nM DHT (shown above gel) and ChIP was performed using PG21 anti-hAR antibody. Precipitated genomic DNA was amplified using primers for the ARE in the hAR 5′ UTR ( ARE ), or in the PSA upstream promoter ( PSA-ARE-III ) with DHT treated cells. Lanes : IP input sample, Ig preimmune rabbit IgG, Ab antibody. Charts display values expressed as percentage of input DNA and represent means ± S.D, * p
    Nhei Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 512 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Vector maps and PEG purification. a Basic vector maps (not to scale) for the original Tet-pLKO-Puro vector and our modified versions. b Agarose gel electrophoresis comparing DNA precipitation methods. 10 μg of EZ-Tet-pLKO vector DNA was co-digested with NheI + EcoRI. The digest was split into three 3 μg aliquots and precipitated with isopropanol (Iso) or polyethylene glycol (PEG) at 6 or 8% concentration. 1 μg of control DNA (uncut and cut) was run alongside 1/3 of the precipitated DNA samples

    Journal: BMC Biotechnology

    Article Title: A streamlined method for the design and cloning of shRNAs into an optimized Dox-inducible lentiviral vector

    doi: 10.1186/s12896-017-0341-x

    Figure Lengend Snippet: Vector maps and PEG purification. a Basic vector maps (not to scale) for the original Tet-pLKO-Puro vector and our modified versions. b Agarose gel electrophoresis comparing DNA precipitation methods. 10 μg of EZ-Tet-pLKO vector DNA was co-digested with NheI + EcoRI. The digest was split into three 3 μg aliquots and precipitated with isopropanol (Iso) or polyethylene glycol (PEG) at 6 or 8% concentration. 1 μg of control DNA (uncut and cut) was run alongside 1/3 of the precipitated DNA samples

    Article Snippet: Vector digest and PEG precipitation Vector can be prepared by co-digesting EZ-Tet-pLKO-Puro DNA with NheI and EcoRI (NEB).

    Techniques: Plasmid Preparation, Purification, Modification, Agarose Gel Electrophoresis, Concentration Assay

    Electrophoresis of pPrime- fliC Recombinant Plasmids From White Colonies on 1% Agarose Gel Lane 1 to 6: extracted Pprime- fliC plasmids; Lane 8 to 13: Pprime- fliC plasmids digested by XhoI; Lane 15 to 20: Pprime- fliC plasmids digested by XhoI and NheI; Lane 7 and 14: 1 Kbp DNA ladder vivantis.

    Journal: Jundishapur Journal of Microbiology

    Article Title: Cloning of fliC Gene From Salmonella typhimurium in the Expression Vector pVAX1 and Evaluation of its Expression in Eukaryotic Cells

    doi: 10.5812/jjm.12351

    Figure Lengend Snippet: Electrophoresis of pPrime- fliC Recombinant Plasmids From White Colonies on 1% Agarose Gel Lane 1 to 6: extracted Pprime- fliC plasmids; Lane 8 to 13: Pprime- fliC plasmids digested by XhoI; Lane 15 to 20: Pprime- fliC plasmids digested by XhoI and NheI; Lane 7 and 14: 1 Kbp DNA ladder vivantis.

    Article Snippet: Subcloning in pVAX1 Eukaryotic Expression Vector The recombinant plasmid pPrime-fliC and eukaryotic expression vector pVAX1 (Invitrogen, Carlsbad, CA, USA) were digested by XhoI and NheI enzymes (New England BioLabs, USA) at 37˚C for 16 hours.

    Techniques: Electrophoresis, Recombinant, Agarose Gel Electrophoresis

    AgaroseGel Electrophoresis of Restriction Enzyme Digestion of Purified pVAX- fliC Recombinant Plasmid Lane 1: pVAX- fliC plasmid digested by XhoI had one band (4419 bp); Lane 2: Double Digestion by NheI and XhoI on pVAX- fliC plasmid had two bands that were 1509 bp (down) and 2910 bp (up); Lane 3: 1 Kbp DNA ladder Vivantis.

    Journal: Jundishapur Journal of Microbiology

    Article Title: Cloning of fliC Gene From Salmonella typhimurium in the Expression Vector pVAX1 and Evaluation of its Expression in Eukaryotic Cells

    doi: 10.5812/jjm.12351

    Figure Lengend Snippet: AgaroseGel Electrophoresis of Restriction Enzyme Digestion of Purified pVAX- fliC Recombinant Plasmid Lane 1: pVAX- fliC plasmid digested by XhoI had one band (4419 bp); Lane 2: Double Digestion by NheI and XhoI on pVAX- fliC plasmid had two bands that were 1509 bp (down) and 2910 bp (up); Lane 3: 1 Kbp DNA ladder Vivantis.

    Article Snippet: Subcloning in pVAX1 Eukaryotic Expression Vector The recombinant plasmid pPrime-fliC and eukaryotic expression vector pVAX1 (Invitrogen, Carlsbad, CA, USA) were digested by XhoI and NheI enzymes (New England BioLabs, USA) at 37˚C for 16 hours.

    Techniques: Electrophoresis, Purification, Recombinant, Plasmid Preparation

    Digestion of 746-bp 23S rRNA obtained by PCR with NheI and separated on 2% agarose gel in 1× Tris-acetate-EDTA buffer. Lane 1, φX174 replicative-form DNA/HaeIII molecular mass standard; lanes 2 and 4, linezolid-susceptible strains SM888 and SM913 (MIC, 2 μg/ml); lanes 3 and 5, strains SM912 and SM925 (MIC, 8 μg/ml); lanes 6 and 7, strains SM943 and SM944 (MIC, 64 μg/ml).

    Journal: Journal of Clinical Microbiology

    Article Title: Emergence of Linezolid Resistance in the Vancomycin-Resistant Enterococcus faecium Multilocus Sequence Typing C1 Epidemic Lineage

    doi: 10.1128/JCM.44.3.1153-1155.2006

    Figure Lengend Snippet: Digestion of 746-bp 23S rRNA obtained by PCR with NheI and separated on 2% agarose gel in 1× Tris-acetate-EDTA buffer. Lane 1, φX174 replicative-form DNA/HaeIII molecular mass standard; lanes 2 and 4, linezolid-susceptible strains SM888 and SM913 (MIC, 2 μg/ml); lanes 3 and 5, strains SM912 and SM925 (MIC, 8 μg/ml); lanes 6 and 7, strains SM943 and SM944 (MIC, 64 μg/ml).

    Article Snippet: The cycling conditions were as follows: an initial denaturation at 94°C for 5 min, followed by 30 cycles consisting of 94°C for 1 min, 50°C for 30 s, 72°C for 1 min, and a final extension step at 72°C for 5 min. Small amounts (5 μl) of PCR 23S rRNA were digested with 10 units of NheI (Invitrogen Life Technologies) at 37°C for 4 h and separated on 2% agarose gel in 1× Tris-acetate-EDTA buffer.

    Techniques: Polymerase Chain Reaction, Agarose Gel Electrophoresis

    Functional differences of the Gli1 5' UTR variants . (A) Schematic representation of the six Gli1 5' UTRs analyzed. The alternative TSSs, the individual exons and the initiator methionine codon are represented as in Figure 2A. Bold lines indicate the six 5' UTR regions, L, M, S, LΔ1B, MΔ1B, SΔ1B, which were cloned into the NheI site, upstream of the Renilla luciferase coding region (black box) of the psiCHECK2 vector as described in methods. (B, C, and D) Luciferase activity of the six reporter constructs after transfection into NIH3T3 cells, Sufu -/- MEFs, and Ptch1 -/- MEFs, respectively. Transfected NIH3T3 cells were treated with methanol (MeOH) or SAG. The Renilla luciferase activity was normalized relative to that of the Firefly luciferase. The error bars indicate the standard deviation. The statistical significance of the differences among the Gli1 5' UTR constructs is shown. (*: p

    Journal: BMC Molecular Biology

    Article Title: Genetic variations regulate alternative splicing in the 5' untranslated regions of the mouse glioma-associated oncogene 1, Gli1

    doi: 10.1186/1471-2199-11-32

    Figure Lengend Snippet: Functional differences of the Gli1 5' UTR variants . (A) Schematic representation of the six Gli1 5' UTRs analyzed. The alternative TSSs, the individual exons and the initiator methionine codon are represented as in Figure 2A. Bold lines indicate the six 5' UTR regions, L, M, S, LΔ1B, MΔ1B, SΔ1B, which were cloned into the NheI site, upstream of the Renilla luciferase coding region (black box) of the psiCHECK2 vector as described in methods. (B, C, and D) Luciferase activity of the six reporter constructs after transfection into NIH3T3 cells, Sufu -/- MEFs, and Ptch1 -/- MEFs, respectively. Transfected NIH3T3 cells were treated with methanol (MeOH) or SAG. The Renilla luciferase activity was normalized relative to that of the Firefly luciferase. The error bars indicate the standard deviation. The statistical significance of the differences among the Gli1 5' UTR constructs is shown. (*: p

    Article Snippet: The PCR products were then digested with the NheI restriction enzyme and cloned upstream of the Renilla luciferase open reading frame of the psiCHECK2 vector (Promega, GenBank: AY535007 ), which is under the control of the SV40 early enhancer/promoter.

    Techniques: Functional Assay, Clone Assay, Luciferase, Plasmid Preparation, Activity Assay, Construct, Transfection, Standard Deviation

    Diagram of vaccine constructs and controls. BNSP333 is the parental vector and FILORAB1, the control used, is based on BNSP333 with a codon-optimized Zaire Ebola Virus Glycoprotein (EBOV GP) inserted between N and P through the BsiWI and NheI restriction digest sites. LASSARAB was generated in a similar manner as FILORAB1, from BNSP333 by cloning a codon-optimized version of Lassa virus glycoprotein (LASV GPC) in the BsiWI and NheI restriction digest sites. LASSARAB-ΔG was further generated from LASSARAB by removing the native rabies glycoprotein (G) by using the restriction digest sites SmaI and PacI. rVSV-GPC was generated by replacing the native VSV glycoprotein (G) by LASV GPC at the same sites. rVSV-GPC was created to be used as a control vector and as a scaffold to produce a native LASV GPC antigen for ELISAs (see Methods section)

    Journal: Nature Communications

    Article Title: Non-neutralizing antibodies elicited by recombinant Lassa–Rabies vaccine are critical for protection against Lassa fever

    doi: 10.1038/s41467-018-06741-w

    Figure Lengend Snippet: Diagram of vaccine constructs and controls. BNSP333 is the parental vector and FILORAB1, the control used, is based on BNSP333 with a codon-optimized Zaire Ebola Virus Glycoprotein (EBOV GP) inserted between N and P through the BsiWI and NheI restriction digest sites. LASSARAB was generated in a similar manner as FILORAB1, from BNSP333 by cloning a codon-optimized version of Lassa virus glycoprotein (LASV GPC) in the BsiWI and NheI restriction digest sites. LASSARAB-ΔG was further generated from LASSARAB by removing the native rabies glycoprotein (G) by using the restriction digest sites SmaI and PacI. rVSV-GPC was generated by replacing the native VSV glycoprotein (G) by LASV GPC at the same sites. rVSV-GPC was created to be used as a control vector and as a scaffold to produce a native LASV GPC antigen for ELISAs (see Methods section)

    Article Snippet: Generation of VSV pseudovirons (ppVSV) To generate ppVSV, the cDNA plasmid backbone of rVSV-GPC was digested with MluI and NheI restriction enzymes to remove the LASV GPC glycoprotein and insert the NanoLuc ORF (Promega).

    Techniques: Construct, Plasmid Preparation, Generated, Clone Assay, Gel Permeation Chromatography

    Linear view of consensus optical maps with DNA sequence-based maps. Solid black arrows represent the locations of missing cuts in the consensus optical maps. The XbaI and NheI consensus optical maps extend to the left of the origin of the DNA sequence-based map because both optical maps had a missing cut at that position.

    Journal: Applied and Environmental Microbiology

    Article Title: Whole-Genome Shotgun Optical Mapping of Rhodospirillum rubrum

    doi: 10.1128/AEM.71.9.5511-5522.2005

    Figure Lengend Snippet: Linear view of consensus optical maps with DNA sequence-based maps. Solid black arrows represent the locations of missing cuts in the consensus optical maps. The XbaI and NheI consensus optical maps extend to the left of the origin of the DNA sequence-based map because both optical maps had a missing cut at that position.

    Article Snippet: Surface properties were assayed by digesting lambda DASH II bacteriophage DNA with 40 units of XbaI, HindIII, and NheI diluted in 200 μl of digestion buffer with 0.2% Triton X-100 (Sigma, St. Louis, MO) at 37°C to determine optimal digestion times, which ranged from 30 to 120 min.

    Techniques: Sequencing

    Use of optical maps in confirming assembly and order of sequence contigs. In silico maps of the sequence contigs were made and aligned against the whole-genome optical maps located in the center of each diagram. A) The high-resolution HindIII map enabled alignment of the small (∼80 kb) sequence contig (contig 83) between contigs 85c and 86c. Aside from the redundant alignment of contigs 87 and 90, no gaps remained in alignment between the optical map and sequence. B) The NheI map gave the clearest representation of the redundant alignment, shown in red in the optical map, of contigs 87 and 90. The alignment of contig 87 had a better match to the optical map than do the rightmost eight fragments in contig 90 (shown in the red square). C) Removing the rightmost eight fragments from contig 90 to make contigs 90a and 90b and reversing the orientation of 90b permitted alignment to the gap between contig 87 and contig 85c. Alignments were made with MapViewer Software (OpGen, Inc, Madison WI.).

    Journal: Applied and Environmental Microbiology

    Article Title: Whole-Genome Shotgun Optical Mapping of Rhodospirillum rubrum

    doi: 10.1128/AEM.71.9.5511-5522.2005

    Figure Lengend Snippet: Use of optical maps in confirming assembly and order of sequence contigs. In silico maps of the sequence contigs were made and aligned against the whole-genome optical maps located in the center of each diagram. A) The high-resolution HindIII map enabled alignment of the small (∼80 kb) sequence contig (contig 83) between contigs 85c and 86c. Aside from the redundant alignment of contigs 87 and 90, no gaps remained in alignment between the optical map and sequence. B) The NheI map gave the clearest representation of the redundant alignment, shown in red in the optical map, of contigs 87 and 90. The alignment of contig 87 had a better match to the optical map than do the rightmost eight fragments in contig 90 (shown in the red square). C) Removing the rightmost eight fragments from contig 90 to make contigs 90a and 90b and reversing the orientation of 90b permitted alignment to the gap between contig 87 and contig 85c. Alignments were made with MapViewer Software (OpGen, Inc, Madison WI.).

    Article Snippet: Surface properties were assayed by digesting lambda DASH II bacteriophage DNA with 40 units of XbaI, HindIII, and NheI diluted in 200 μl of digestion buffer with 0.2% Triton X-100 (Sigma, St. Louis, MO) at 37°C to determine optimal digestion times, which ranged from 30 to 120 min.

    Techniques: Sequencing, In Silico, Software

    Comparisons of the XbaI, NheI, and HindIII optical maps to sequence data. (A, B, and C) Plots of optical map fragment sizes versus the DNA sequence-based map fragment sizes from the finished sequence for XbaI (A), NheI (B), and HindIII (C). The error bars represent the standard deviation of optical map fragment size about the mean. (D, E, F) Plots of the relative fragment sizing error [100 × (optical map fragment size - corresponding DNA sequence-based map fragment size)/corresponding DNA sequence-based map fragment size] versus DNA sequence-based map fragment size for XbaI ( D), NheI (E), and HindIII (F).

    Journal: Applied and Environmental Microbiology

    Article Title: Whole-Genome Shotgun Optical Mapping of Rhodospirillum rubrum

    doi: 10.1128/AEM.71.9.5511-5522.2005

    Figure Lengend Snippet: Comparisons of the XbaI, NheI, and HindIII optical maps to sequence data. (A, B, and C) Plots of optical map fragment sizes versus the DNA sequence-based map fragment sizes from the finished sequence for XbaI (A), NheI (B), and HindIII (C). The error bars represent the standard deviation of optical map fragment size about the mean. (D, E, F) Plots of the relative fragment sizing error [100 × (optical map fragment size - corresponding DNA sequence-based map fragment size)/corresponding DNA sequence-based map fragment size] versus DNA sequence-based map fragment size for XbaI ( D), NheI (E), and HindIII (F).

    Article Snippet: Surface properties were assayed by digesting lambda DASH II bacteriophage DNA with 40 units of XbaI, HindIII, and NheI diluted in 200 μl of digestion buffer with 0.2% Triton X-100 (Sigma, St. Louis, MO) at 37°C to determine optimal digestion times, which ranged from 30 to 120 min.

    Techniques: Sequencing, Standard Deviation

    ChIP analysis confirms binding of hAR to 5′ UTR ARE . a Line diagram (not to scale) of the hAR 5′ UTR showing the recognition sites of the restriction endonucleases NheI and PvuII used to digest chromatin and plasmid, plus the forward (F) and reverse (R) primers ( solid arrows ) used for ChIP semiquantitative PCR. Oligonucleotides Gen-R and Vect-R are specific for the genomic and plasmid vector sequences, respectively, and the bent arrow indicates the transcriptional start site. b , c and d Representative agarose gels of PCR amplified immunoprecipitated DNA. b LNCaP cells were treated with either vehicle or 10 nM DHT (shown above gel) and ChIP was performed using PG21 anti-hAR antibody. Precipitated genomic DNA was amplified using primers for the ARE in the hAR 5′ UTR ( ARE ), or in the PSA upstream promoter ( PSA-ARE-III ) with DHT treated cells. Lanes : IP input sample, Ig preimmune rabbit IgG, Ab antibody. Charts display values expressed as percentage of input DNA and represent means ± S.D, * p

    Journal: Hormones & Cancer

    Article Title: Negative Regulation of the Androgen Receptor Gene Through a Primate-Specific Androgen Response Element Present in the 5′ UTR

    doi: 10.1007/s12672-014-0185-y

    Figure Lengend Snippet: ChIP analysis confirms binding of hAR to 5′ UTR ARE . a Line diagram (not to scale) of the hAR 5′ UTR showing the recognition sites of the restriction endonucleases NheI and PvuII used to digest chromatin and plasmid, plus the forward (F) and reverse (R) primers ( solid arrows ) used for ChIP semiquantitative PCR. Oligonucleotides Gen-R and Vect-R are specific for the genomic and plasmid vector sequences, respectively, and the bent arrow indicates the transcriptional start site. b , c and d Representative agarose gels of PCR amplified immunoprecipitated DNA. b LNCaP cells were treated with either vehicle or 10 nM DHT (shown above gel) and ChIP was performed using PG21 anti-hAR antibody. Precipitated genomic DNA was amplified using primers for the ARE in the hAR 5′ UTR ( ARE ), or in the PSA upstream promoter ( PSA-ARE-III ) with DHT treated cells. Lanes : IP input sample, Ig preimmune rabbit IgG, Ab antibody. Charts display values expressed as percentage of input DNA and represent means ± S.D, * p

    Article Snippet: Chromatin and plasmid were digested with 400 units each PvuII (NEB) and NheI (Roche) for 15 min at 37 °C; followed by lysis and the removal of insoluble debris by centrifugation.

    Techniques: Chromatin Immunoprecipitation, Binding Assay, Plasmid Preparation, Polymerase Chain Reaction, Amplification, Immunoprecipitation