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  • 92
    BioShop nh4 cl
    Nh4 Cl, supplied by BioShop, used in various techniques. Bioz Stars score: 92/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cambridge Isotope Laboratories nh4 cl
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    Fisher Scientific nh4 cl
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    Millipore nh4 cl
    γ-Secretase inhibitor treatment triggers autophagic dysfunction in APPswe-expressing SHSY-5Y but not mock-transfected cells. a SH-APPswe cells were treated with ELND006 (D6) and analyzed for APP-CTF levels by western blot. APP-CTFs were detected using α-APPct, 4G8 or 6E10 (see diagram for epitope recognition) and compared to C99-flag expression in HEK transfected cells. b Co-immunocytochemical staining of vehicle (Veh) or D6-treated APPswe cells using α-APPct and α-lamp1 shows a high overlap of these labelings. Scale bar 2 μm. c Vehicle (V) or D6-treated SH-mock or SH-APPswe cells were stained with Lysotracker red and DAPI. Scale bar 5 μm. d Cathepsin B (CatB) activity was monitored in vitro from microsomal fractions of SH-APPswe cells treated with vehicle (V), D6, DAPT (DT) or NH 4 Cl <t>(NH4).</t> Data are represented as mean ± SEM, as determined by ANOVA one-way Dunnett post hoc test, *** p
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    Nacalai nh4 cl
    γ-Secretase inhibitor treatment triggers autophagic dysfunction in APPswe-expressing SHSY-5Y but not mock-transfected cells. a SH-APPswe cells were treated with ELND006 (D6) and analyzed for APP-CTF levels by western blot. APP-CTFs were detected using α-APPct, 4G8 or 6E10 (see diagram for epitope recognition) and compared to C99-flag expression in HEK transfected cells. b Co-immunocytochemical staining of vehicle (Veh) or D6-treated APPswe cells using α-APPct and α-lamp1 shows a high overlap of these labelings. Scale bar 2 μm. c Vehicle (V) or D6-treated SH-mock or SH-APPswe cells were stained with Lysotracker red and DAPI. Scale bar 5 μm. d Cathepsin B (CatB) activity was monitored in vitro from microsomal fractions of SH-APPswe cells treated with vehicle (V), D6, DAPT (DT) or NH 4 Cl <t>(NH4).</t> Data are represented as mean ± SEM, as determined by ANOVA one-way Dunnett post hoc test, *** p
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    Sangon Biotech nh4 cl
    γ-Secretase inhibitor treatment triggers autophagic dysfunction in APPswe-expressing SHSY-5Y but not mock-transfected cells. a SH-APPswe cells were treated with ELND006 (D6) and analyzed for APP-CTF levels by western blot. APP-CTFs were detected using α-APPct, 4G8 or 6E10 (see diagram for epitope recognition) and compared to C99-flag expression in HEK transfected cells. b Co-immunocytochemical staining of vehicle (Veh) or D6-treated APPswe cells using α-APPct and α-lamp1 shows a high overlap of these labelings. Scale bar 2 μm. c Vehicle (V) or D6-treated SH-mock or SH-APPswe cells were stained with Lysotracker red and DAPI. Scale bar 5 μm. d Cathepsin B (CatB) activity was monitored in vitro from microsomal fractions of SH-APPswe cells treated with vehicle (V), D6, DAPT (DT) or NH 4 Cl <t>(NH4).</t> Data are represented as mean ± SEM, as determined by ANOVA one-way Dunnett post hoc test, *** p
    Nh4 Cl, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    STEMCELL Technologies Inc nh4 cl
    γ-Secretase inhibitor treatment triggers autophagic dysfunction in APPswe-expressing SHSY-5Y but not mock-transfected cells. a SH-APPswe cells were treated with ELND006 (D6) and analyzed for APP-CTF levels by western blot. APP-CTFs were detected using α-APPct, 4G8 or 6E10 (see diagram for epitope recognition) and compared to C99-flag expression in HEK transfected cells. b Co-immunocytochemical staining of vehicle (Veh) or D6-treated APPswe cells using α-APPct and α-lamp1 shows a high overlap of these labelings. Scale bar 2 μm. c Vehicle (V) or D6-treated SH-mock or SH-APPswe cells were stained with Lysotracker red and DAPI. Scale bar 5 μm. d Cathepsin B (CatB) activity was monitored in vitro from microsomal fractions of SH-APPswe cells treated with vehicle (V), D6, DAPT (DT) or NH 4 Cl <t>(NH4).</t> Data are represented as mean ± SEM, as determined by ANOVA one-way Dunnett post hoc test, *** p
    Nh4 Cl, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 92/100, based on 117 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher nh4 cl
    γ-Secretase inhibitor treatment triggers autophagic dysfunction in APPswe-expressing SHSY-5Y but not mock-transfected cells. a SH-APPswe cells were treated with ELND006 (D6) and analyzed for APP-CTF levels by western blot. APP-CTFs were detected using α-APPct, 4G8 or 6E10 (see diagram for epitope recognition) and compared to C99-flag expression in HEK transfected cells. b Co-immunocytochemical staining of vehicle (Veh) or D6-treated APPswe cells using α-APPct and α-lamp1 shows a high overlap of these labelings. Scale bar 2 μm. c Vehicle (V) or D6-treated SH-mock or SH-APPswe cells were stained with Lysotracker red and DAPI. Scale bar 5 μm. d Cathepsin B (CatB) activity was monitored in vitro from microsomal fractions of SH-APPswe cells treated with vehicle (V), D6, DAPT (DT) or NH 4 Cl <t>(NH4).</t> Data are represented as mean ± SEM, as determined by ANOVA one-way Dunnett post hoc test, *** p
    Nh4 Cl, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1100 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Applichem nh4 cl
    γ-Secretase inhibitor treatment triggers autophagic dysfunction in APPswe-expressing SHSY-5Y but not mock-transfected cells. a SH-APPswe cells were treated with ELND006 (D6) and analyzed for APP-CTF levels by western blot. APP-CTFs were detected using α-APPct, 4G8 or 6E10 (see diagram for epitope recognition) and compared to C99-flag expression in HEK transfected cells. b Co-immunocytochemical staining of vehicle (Veh) or D6-treated APPswe cells using α-APPct and α-lamp1 shows a high overlap of these labelings. Scale bar 2 μm. c Vehicle (V) or D6-treated SH-mock or SH-APPswe cells were stained with Lysotracker red and DAPI. Scale bar 5 μm. d Cathepsin B (CatB) activity was monitored in vitro from microsomal fractions of SH-APPswe cells treated with vehicle (V), D6, DAPT (DT) or NH 4 Cl <t>(NH4).</t> Data are represented as mean ± SEM, as determined by ANOVA one-way Dunnett post hoc test, *** p
    Nh4 Cl, supplied by Applichem, used in various techniques. Bioz Stars score: 92/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Avantor nh4 cl
    γ-Secretase inhibitor treatment triggers autophagic dysfunction in APPswe-expressing SHSY-5Y but not mock-transfected cells. a SH-APPswe cells were treated with ELND006 (D6) and analyzed for APP-CTF levels by western blot. APP-CTFs were detected using α-APPct, 4G8 or 6E10 (see diagram for epitope recognition) and compared to C99-flag expression in HEK transfected cells. b Co-immunocytochemical staining of vehicle (Veh) or D6-treated APPswe cells using α-APPct and α-lamp1 shows a high overlap of these labelings. Scale bar 2 μm. c Vehicle (V) or D6-treated SH-mock or SH-APPswe cells were stained with Lysotracker red and DAPI. Scale bar 5 μm. d Cathepsin B (CatB) activity was monitored in vitro from microsomal fractions of SH-APPswe cells treated with vehicle (V), D6, DAPT (DT) or NH 4 Cl <t>(NH4).</t> Data are represented as mean ± SEM, as determined by ANOVA one-way Dunnett post hoc test, *** p
    Nh4 Cl, supplied by Avantor, used in various techniques. Bioz Stars score: 92/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Isotec nh4 cl
    γ-Secretase inhibitor treatment triggers autophagic dysfunction in APPswe-expressing SHSY-5Y but not mock-transfected cells. a SH-APPswe cells were treated with ELND006 (D6) and analyzed for APP-CTF levels by western blot. APP-CTFs were detected using α-APPct, 4G8 or 6E10 (see diagram for epitope recognition) and compared to C99-flag expression in HEK transfected cells. b Co-immunocytochemical staining of vehicle (Veh) or D6-treated APPswe cells using α-APPct and α-lamp1 shows a high overlap of these labelings. Scale bar 2 μm. c Vehicle (V) or D6-treated SH-mock or SH-APPswe cells were stained with Lysotracker red and DAPI. Scale bar 5 μm. d Cathepsin B (CatB) activity was monitored in vitro from microsomal fractions of SH-APPswe cells treated with vehicle (V), D6, DAPT (DT) or NH 4 Cl <t>(NH4).</t> Data are represented as mean ± SEM, as determined by ANOVA one-way Dunnett post hoc test, *** p
    Nh4 Cl, supplied by Isotec, used in various techniques. Bioz Stars score: 92/100, based on 373 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Martek Biosciences nh4 cl
    γ-Secretase inhibitor treatment triggers autophagic dysfunction in APPswe-expressing SHSY-5Y but not mock-transfected cells. a SH-APPswe cells were treated with ELND006 (D6) and analyzed for APP-CTF levels by western blot. APP-CTFs were detected using α-APPct, 4G8 or 6E10 (see diagram for epitope recognition) and compared to C99-flag expression in HEK transfected cells. b Co-immunocytochemical staining of vehicle (Veh) or D6-treated APPswe cells using α-APPct and α-lamp1 shows a high overlap of these labelings. Scale bar 2 μm. c Vehicle (V) or D6-treated SH-mock or SH-APPswe cells were stained with Lysotracker red and DAPI. Scale bar 5 μm. d Cathepsin B (CatB) activity was monitored in vitro from microsomal fractions of SH-APPswe cells treated with vehicle (V), D6, DAPT (DT) or NH 4 Cl <t>(NH4).</t> Data are represented as mean ± SEM, as determined by ANOVA one-way Dunnett post hoc test, *** p
    Nh4 Cl, supplied by Martek Biosciences, used in various techniques. Bioz Stars score: 92/100, based on 87 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA nh4 cl
    Phylogenetic tree of OTU 0.03 s related to taxa of <t>NH4</t> + -oxidizing and NO 2 − -oxidizing prokaryotes in the investigated filter material samples. The tree was constructed using the neighbor-joining algorithm in ARB with 1,000 bootstrap replicates. The percentage of replicate trees ( > 60%) are shown by thick branches. The scale bar represents 0.10 substitutions per nucleotide position. The numbers in parentheses refer to the observed sequences in each OTU 0.03 . Filter grain fractions are as indicated on the figure. seq, sequence(s).
    Nh4 Cl, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 117 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Carl Roth GmbH nh4 cl
    Phylogenetic tree of OTU 0.03 s related to taxa of <t>NH4</t> + -oxidizing and NO 2 − -oxidizing prokaryotes in the investigated filter material samples. The tree was constructed using the neighbor-joining algorithm in ARB with 1,000 bootstrap replicates. The percentage of replicate trees ( > 60%) are shown by thick branches. The scale bar represents 0.10 substitutions per nucleotide position. The numbers in parentheses refer to the observed sequences in each OTU 0.03 . Filter grain fractions are as indicated on the figure. seq, sequence(s).
    Nh4 Cl, supplied by Carl Roth GmbH, used in various techniques. Bioz Stars score: 92/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Hospira nh4 cl
    Phylogenetic tree of OTU 0.03 s related to taxa of <t>NH4</t> + -oxidizing and NO 2 − -oxidizing prokaryotes in the investigated filter material samples. The tree was constructed using the neighbor-joining algorithm in ARB with 1,000 bootstrap replicates. The percentage of replicate trees ( > 60%) are shown by thick branches. The scale bar represents 0.10 substitutions per nucleotide position. The numbers in parentheses refer to the observed sequences in each OTU 0.03 . Filter grain fractions are as indicated on the figure. seq, sequence(s).
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    FUJIFILM nh4 cl
    Phylogenetic tree of OTU 0.03 s related to taxa of <t>NH4</t> + -oxidizing and NO 2 − -oxidizing prokaryotes in the investigated filter material samples. The tree was constructed using the neighbor-joining algorithm in ARB with 1,000 bootstrap replicates. The percentage of replicate trees ( > 60%) are shown by thick branches. The scale bar represents 0.10 substitutions per nucleotide position. The numbers in parentheses refer to the observed sequences in each OTU 0.03 . Filter grain fractions are as indicated on the figure. seq, sequence(s).
    Nh4 Cl, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 92/100, based on 71 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Beyotime nh4 cl
    Phylogenetic tree of OTU 0.03 s related to taxa of <t>NH4</t> + -oxidizing and NO 2 − -oxidizing prokaryotes in the investigated filter material samples. The tree was constructed using the neighbor-joining algorithm in ARB with 1,000 bootstrap replicates. The percentage of replicate trees ( > 60%) are shown by thick branches. The scale bar represents 0.10 substitutions per nucleotide position. The numbers in parentheses refer to the observed sequences in each OTU 0.03 . Filter grain fractions are as indicated on the figure. seq, sequence(s).
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    Cell Signaling Technology Inc nh4 cl
    Phylogenetic tree of OTU 0.03 s related to taxa of <t>NH4</t> + -oxidizing and NO 2 − -oxidizing prokaryotes in the investigated filter material samples. The tree was constructed using the neighbor-joining algorithm in ARB with 1,000 bootstrap replicates. The percentage of replicate trees ( > 60%) are shown by thick branches. The scale bar represents 0.10 substitutions per nucleotide position. The numbers in parentheses refer to the observed sequences in each OTU 0.03 . Filter grain fractions are as indicated on the figure. seq, sequence(s).
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    Melford Laboratories nh4 cl
    Phylogenetic tree of OTU 0.03 s related to taxa of <t>NH4</t> + -oxidizing and NO 2 − -oxidizing prokaryotes in the investigated filter material samples. The tree was constructed using the neighbor-joining algorithm in ARB with 1,000 bootstrap replicates. The percentage of replicate trees ( > 60%) are shown by thick branches. The scale bar represents 0.10 substitutions per nucleotide position. The numbers in parentheses refer to the observed sequences in each OTU 0.03 . Filter grain fractions are as indicated on the figure. seq, sequence(s).
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    Campro Scientific nh4 cl
    Phylogenetic tree of OTU 0.03 s related to taxa of <t>NH4</t> + -oxidizing and NO 2 − -oxidizing prokaryotes in the investigated filter material samples. The tree was constructed using the neighbor-joining algorithm in ARB with 1,000 bootstrap replicates. The percentage of replicate trees ( > 60%) are shown by thick branches. The scale bar represents 0.10 substitutions per nucleotide position. The numbers in parentheses refer to the observed sequences in each OTU 0.03 . Filter grain fractions are as indicated on the figure. seq, sequence(s).
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    Amresco nh4 cl
    Phylogenetic tree of OTU 0.03 s related to taxa of <t>NH4</t> + -oxidizing and NO 2 − -oxidizing prokaryotes in the investigated filter material samples. The tree was constructed using the neighbor-joining algorithm in ARB with 1,000 bootstrap replicates. The percentage of replicate trees ( > 60%) are shown by thick branches. The scale bar represents 0.10 substitutions per nucleotide position. The numbers in parentheses refer to the observed sequences in each OTU 0.03 . Filter grain fractions are as indicated on the figure. seq, sequence(s).
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    Teknova nh4 cl
    Phylogenetic tree of OTU 0.03 s related to taxa of <t>NH4</t> + -oxidizing and NO 2 − -oxidizing prokaryotes in the investigated filter material samples. The tree was constructed using the neighbor-joining algorithm in ARB with 1,000 bootstrap replicates. The percentage of replicate trees ( > 60%) are shown by thick branches. The scale bar represents 0.10 substitutions per nucleotide position. The numbers in parentheses refer to the observed sequences in each OTU 0.03 . Filter grain fractions are as indicated on the figure. seq, sequence(s).
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    Millipore nh4 cl buffer
    Phylogenetic tree of OTU 0.03 s related to taxa of <t>NH4</t> + -oxidizing and NO 2 − -oxidizing prokaryotes in the investigated filter material samples. The tree was constructed using the neighbor-joining algorithm in ARB with 1,000 bootstrap replicates. The percentage of replicate trees ( > 60%) are shown by thick branches. The scale bar represents 0.10 substitutions per nucleotide position. The numbers in parentheses refer to the observed sequences in each OTU 0.03 . Filter grain fractions are as indicated on the figure. seq, sequence(s).
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    Cambridge Isotope Laboratories n nh4 cl
    Phylogenetic tree of OTU 0.03 s related to taxa of <t>NH4</t> + -oxidizing and NO 2 − -oxidizing prokaryotes in the investigated filter material samples. The tree was constructed using the neighbor-joining algorithm in ARB with 1,000 bootstrap replicates. The percentage of replicate trees ( > 60%) are shown by thick branches. The scale bar represents 0.10 substitutions per nucleotide position. The numbers in parentheses refer to the observed sequences in each OTU 0.03 . Filter grain fractions are as indicated on the figure. seq, sequence(s).
    N Nh4 Cl, supplied by Cambridge Isotope Laboratories, used in various techniques. Bioz Stars score: 91/100, based on 751 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    γ-Secretase inhibitor treatment triggers autophagic dysfunction in APPswe-expressing SHSY-5Y but not mock-transfected cells. a SH-APPswe cells were treated with ELND006 (D6) and analyzed for APP-CTF levels by western blot. APP-CTFs were detected using α-APPct, 4G8 or 6E10 (see diagram for epitope recognition) and compared to C99-flag expression in HEK transfected cells. b Co-immunocytochemical staining of vehicle (Veh) or D6-treated APPswe cells using α-APPct and α-lamp1 shows a high overlap of these labelings. Scale bar 2 μm. c Vehicle (V) or D6-treated SH-mock or SH-APPswe cells were stained with Lysotracker red and DAPI. Scale bar 5 μm. d Cathepsin B (CatB) activity was monitored in vitro from microsomal fractions of SH-APPswe cells treated with vehicle (V), D6, DAPT (DT) or NH 4 Cl (NH4). Data are represented as mean ± SEM, as determined by ANOVA one-way Dunnett post hoc test, *** p

    Journal: Acta Neuropathologica

    Article Title: Intraneuronal aggregation of the β-CTF fragment of APP (C99) induces Aβ-independent lysosomal-autophagic pathology

    doi: 10.1007/s00401-016-1577-6

    Figure Lengend Snippet: γ-Secretase inhibitor treatment triggers autophagic dysfunction in APPswe-expressing SHSY-5Y but not mock-transfected cells. a SH-APPswe cells were treated with ELND006 (D6) and analyzed for APP-CTF levels by western blot. APP-CTFs were detected using α-APPct, 4G8 or 6E10 (see diagram for epitope recognition) and compared to C99-flag expression in HEK transfected cells. b Co-immunocytochemical staining of vehicle (Veh) or D6-treated APPswe cells using α-APPct and α-lamp1 shows a high overlap of these labelings. Scale bar 2 μm. c Vehicle (V) or D6-treated SH-mock or SH-APPswe cells were stained with Lysotracker red and DAPI. Scale bar 5 μm. d Cathepsin B (CatB) activity was monitored in vitro from microsomal fractions of SH-APPswe cells treated with vehicle (V), D6, DAPT (DT) or NH 4 Cl (NH4). Data are represented as mean ± SEM, as determined by ANOVA one-way Dunnett post hoc test, *** p

    Article Snippet: Cell culture and experimental treatments The human neuroblastoma cell line SH-SY5Y, naive or stably expressing APPswe or pcDNA3 (mock), previously described [ ] was exposed to the following drugs for 16–20 h: D6 (50 nM to 5 μM, Elan Pharmaceuticals, San Francisco) in vehicle (methylcellulose/polysorbate 80, Sigma), DAPT (5 μM in DMSO, Sigma), leupeptin (10 μM in H2 O, Sigma), PADK (5 μM in DMSO, Bachem), NH4 Cl (10 mM in H2 O, Sigma), pepstatin A (10 μM in EtOH, Enzo Life sciences), smer28 (50 μM in DMSO, Sigma) and bafilomycin A1 (50 nM in H20, Sigma).

    Techniques: Expressing, Transfection, Western Blot, Staining, Activity Assay, In Vitro

    Phylogenetic tree of OTU 0.03 s related to taxa of NH4 + -oxidizing and NO 2 − -oxidizing prokaryotes in the investigated filter material samples. The tree was constructed using the neighbor-joining algorithm in ARB with 1,000 bootstrap replicates. The percentage of replicate trees ( > 60%) are shown by thick branches. The scale bar represents 0.10 substitutions per nucleotide position. The numbers in parentheses refer to the observed sequences in each OTU 0.03 . Filter grain fractions are as indicated on the figure. seq, sequence(s).

    Journal: Applied and Environmental Microbiology

    Article Title: Internal Porosity of Mineral Coating Supports Microbial Activity in Rapid Sand Filters for Groundwater Treatment

    doi: 10.1128/AEM.01959-14

    Figure Lengend Snippet: Phylogenetic tree of OTU 0.03 s related to taxa of NH4 + -oxidizing and NO 2 − -oxidizing prokaryotes in the investigated filter material samples. The tree was constructed using the neighbor-joining algorithm in ARB with 1,000 bootstrap replicates. The percentage of replicate trees ( > 60%) are shown by thick branches. The scale bar represents 0.10 substitutions per nucleotide position. The numbers in parentheses refer to the observed sequences in each OTU 0.03 . Filter grain fractions are as indicated on the figure. seq, sequence(s).

    Article Snippet: Effluent water from the investigated full-scale rapid sand filter was used for the column influents, supplemented with 1 mg/liter NH4 + -N as NH4 Cl (Merck Chemicals).

    Techniques: Construct, Sequencing