nh4 2 so4 Search Results


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  • 95
    Millipore nh4
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    nh4  (Difco)
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    γ-Secretase inhibitor treatment triggers autophagic dysfunction in APPswe-expressing SHSY-5Y but not mock-transfected cells. a SH-APPswe cells were treated with ELND006 (D6) and analyzed for APP-CTF levels by western blot. APP-CTFs were detected using α-APPct, 4G8 or 6E10 (see diagram for epitope recognition) and compared to C99-flag expression in HEK transfected cells. b Co-immunocytochemical staining of vehicle (Veh) or D6-treated APPswe cells using α-APPct and α-lamp1 shows a high overlap of these labelings. Scale bar 2 μm. c Vehicle (V) or D6-treated SH-mock or SH-APPswe cells were stained with Lysotracker red and DAPI. Scale bar 5 μm. d Cathepsin B (CatB) activity was monitored in vitro from microsomal fractions of SH-APPswe cells treated with vehicle (V), D6, DAPT (DT) or NH 4 Cl <t>(NH4).</t> Data are represented as mean ± SEM, as determined by ANOVA one-way Dunnett post hoc test, *** p
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    γ-Secretase inhibitor treatment triggers autophagic dysfunction in APPswe-expressing SHSY-5Y but not mock-transfected cells. a SH-APPswe cells were treated with ELND006 (D6) and analyzed for APP-CTF levels by western blot. APP-CTFs were detected using α-APPct, 4G8 or 6E10 (see diagram for epitope recognition) and compared to C99-flag expression in HEK transfected cells. b Co-immunocytochemical staining of vehicle (Veh) or D6-treated APPswe cells using α-APPct and α-lamp1 shows a high overlap of these labelings. Scale bar 2 μm. c Vehicle (V) or D6-treated SH-mock or SH-APPswe cells were stained with Lysotracker red and DAPI. Scale bar 5 μm. d Cathepsin B (CatB) activity was monitored in vitro from microsomal fractions of SH-APPswe cells treated with vehicle (V), D6, DAPT (DT) or NH 4 Cl <t>(NH4).</t> Data are represented as mean ± SEM, as determined by ANOVA one-way Dunnett post hoc test, *** p
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    γ-Secretase inhibitor treatment triggers autophagic dysfunction in APPswe-expressing SHSY-5Y but not mock-transfected cells. a SH-APPswe cells were treated with ELND006 (D6) and analyzed for APP-CTF levels by western blot. APP-CTFs were detected using α-APPct, 4G8 or 6E10 (see diagram for epitope recognition) and compared to C99-flag expression in HEK transfected cells. b Co-immunocytochemical staining of vehicle (Veh) or D6-treated APPswe cells using α-APPct and α-lamp1 shows a high overlap of these labelings. Scale bar 2 μm. c Vehicle (V) or D6-treated SH-mock or SH-APPswe cells were stained with Lysotracker red and DAPI. Scale bar 5 μm. d Cathepsin B (CatB) activity was monitored in vitro from microsomal fractions of SH-APPswe cells treated with vehicle (V), D6, DAPT (DT) or NH 4 Cl <t>(NH4).</t> Data are represented as mean ± SEM, as determined by ANOVA one-way Dunnett post hoc test, *** p
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    γ-Secretase inhibitor treatment triggers autophagic dysfunction in APPswe-expressing SHSY-5Y but not mock-transfected cells. a SH-APPswe cells were treated with ELND006 (D6) and analyzed for APP-CTF levels by western blot. APP-CTFs were detected using α-APPct, 4G8 or 6E10 (see diagram for epitope recognition) and compared to C99-flag expression in HEK transfected cells. b Co-immunocytochemical staining of vehicle (Veh) or D6-treated APPswe cells using α-APPct and α-lamp1 shows a high overlap of these labelings. Scale bar 2 μm. c Vehicle (V) or D6-treated SH-mock or SH-APPswe cells were stained with Lysotracker red and DAPI. Scale bar 5 μm. d Cathepsin B (CatB) activity was monitored in vitro from microsomal fractions of SH-APPswe cells treated with vehicle (V), D6, DAPT (DT) or NH 4 Cl <t>(NH4).</t> Data are represented as mean ± SEM, as determined by ANOVA one-way Dunnett post hoc test, *** p
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    γ-Secretase inhibitor treatment triggers autophagic dysfunction in APPswe-expressing SHSY-5Y but not mock-transfected cells. a SH-APPswe cells were treated with ELND006 (D6) and analyzed for APP-CTF levels by western blot. APP-CTFs were detected using α-APPct, 4G8 or 6E10 (see diagram for epitope recognition) and compared to C99-flag expression in HEK transfected cells. b Co-immunocytochemical staining of vehicle (Veh) or D6-treated APPswe cells using α-APPct and α-lamp1 shows a high overlap of these labelings. Scale bar 2 μm. c Vehicle (V) or D6-treated SH-mock or SH-APPswe cells were stained with Lysotracker red and DAPI. Scale bar 5 μm. d Cathepsin B (CatB) activity was monitored in vitro from microsomal fractions of SH-APPswe cells treated with vehicle (V), D6, DAPT (DT) or NH 4 Cl <t>(NH4).</t> Data are represented as mean ± SEM, as determined by ANOVA one-way Dunnett post hoc test, *** p
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    Thermo Fisher nh4 cl
    γ-Secretase inhibitor treatment triggers autophagic dysfunction in APPswe-expressing SHSY-5Y but not mock-transfected cells. a SH-APPswe cells were treated with ELND006 (D6) and analyzed for APP-CTF levels by western blot. APP-CTFs were detected using α-APPct, 4G8 or 6E10 (see diagram for epitope recognition) and compared to C99-flag expression in HEK transfected cells. b Co-immunocytochemical staining of vehicle (Veh) or D6-treated APPswe cells using α-APPct and α-lamp1 shows a high overlap of these labelings. Scale bar 2 μm. c Vehicle (V) or D6-treated SH-mock or SH-APPswe cells were stained with Lysotracker red and DAPI. Scale bar 5 μm. d Cathepsin B (CatB) activity was monitored in vitro from microsomal fractions of SH-APPswe cells treated with vehicle (V), D6, DAPT (DT) or NH 4 Cl <t>(NH4).</t> Data are represented as mean ± SEM, as determined by ANOVA one-way Dunnett post hoc test, *** p
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    γ-Secretase inhibitor treatment triggers autophagic dysfunction in APPswe-expressing SHSY-5Y but not mock-transfected cells. a SH-APPswe cells were treated with ELND006 (D6) and analyzed for APP-CTF levels by western blot. APP-CTFs were detected using α-APPct, 4G8 or 6E10 (see diagram for epitope recognition) and compared to C99-flag expression in HEK transfected cells. b Co-immunocytochemical staining of vehicle (Veh) or D6-treated APPswe cells using α-APPct and α-lamp1 shows a high overlap of these labelings. Scale bar 2 μm. c Vehicle (V) or D6-treated SH-mock or SH-APPswe cells were stained with Lysotracker red and DAPI. Scale bar 5 μm. d Cathepsin B (CatB) activity was monitored in vitro from microsomal fractions of SH-APPswe cells treated with vehicle (V), D6, DAPT (DT) or NH 4 Cl <t>(NH4).</t> Data are represented as mean ± SEM, as determined by ANOVA one-way Dunnett post hoc test, *** p
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    γ-Secretase inhibitor treatment triggers autophagic dysfunction in APPswe-expressing SHSY-5Y but not mock-transfected cells. a SH-APPswe cells were treated with ELND006 (D6) and analyzed for APP-CTF levels by western blot. APP-CTFs were detected using α-APPct, 4G8 or 6E10 (see diagram for epitope recognition) and compared to C99-flag expression in HEK transfected cells. b Co-immunocytochemical staining of vehicle (Veh) or D6-treated APPswe cells using α-APPct and α-lamp1 shows a high overlap of these labelings. Scale bar 2 μm. c Vehicle (V) or D6-treated SH-mock or SH-APPswe cells were stained with Lysotracker red and DAPI. Scale bar 5 μm. d Cathepsin B (CatB) activity was monitored in vitro from microsomal fractions of SH-APPswe cells treated with vehicle (V), D6, DAPT (DT) or NH 4 Cl <t>(NH4).</t> Data are represented as mean ± SEM, as determined by ANOVA one-way Dunnett post hoc test, *** p
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    γ-Secretase inhibitor treatment triggers autophagic dysfunction in APPswe-expressing SHSY-5Y but not mock-transfected cells. a SH-APPswe cells were treated with ELND006 (D6) and analyzed for APP-CTF levels by western blot. APP-CTFs were detected using α-APPct, 4G8 or 6E10 (see diagram for epitope recognition) and compared to C99-flag expression in HEK transfected cells. b Co-immunocytochemical staining of vehicle (Veh) or D6-treated APPswe cells using α-APPct and α-lamp1 shows a high overlap of these labelings. Scale bar 2 μm. c Vehicle (V) or D6-treated SH-mock or SH-APPswe cells were stained with Lysotracker red and DAPI. Scale bar 5 μm. d Cathepsin B (CatB) activity was monitored in vitro from microsomal fractions of SH-APPswe cells treated with vehicle (V), D6, DAPT (DT) or NH 4 Cl <t>(NH4).</t> Data are represented as mean ± SEM, as determined by ANOVA one-way Dunnett post hoc test, *** p
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    Sangon Biotech nh4 cl
    γ-Secretase inhibitor treatment triggers autophagic dysfunction in APPswe-expressing SHSY-5Y but not mock-transfected cells. a SH-APPswe cells were treated with ELND006 (D6) and analyzed for APP-CTF levels by western blot. APP-CTFs were detected using α-APPct, 4G8 or 6E10 (see diagram for epitope recognition) and compared to C99-flag expression in HEK transfected cells. b Co-immunocytochemical staining of vehicle (Veh) or D6-treated APPswe cells using α-APPct and α-lamp1 shows a high overlap of these labelings. Scale bar 2 μm. c Vehicle (V) or D6-treated SH-mock or SH-APPswe cells were stained with Lysotracker red and DAPI. Scale bar 5 μm. d Cathepsin B (CatB) activity was monitored in vitro from microsomal fractions of SH-APPswe cells treated with vehicle (V), D6, DAPT (DT) or NH 4 Cl <t>(NH4).</t> Data are represented as mean ± SEM, as determined by ANOVA one-way Dunnett post hoc test, *** p
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    γ-Secretase inhibitor treatment triggers autophagic dysfunction in APPswe-expressing SHSY-5Y but not mock-transfected cells. a SH-APPswe cells were treated with ELND006 (D6) and analyzed for APP-CTF levels by western blot. APP-CTFs were detected using α-APPct, 4G8 or 6E10 (see diagram for epitope recognition) and compared to C99-flag expression in HEK transfected cells. b Co-immunocytochemical staining of vehicle (Veh) or D6-treated APPswe cells using α-APPct and α-lamp1 shows a high overlap of these labelings. Scale bar 2 μm. c Vehicle (V) or D6-treated SH-mock or SH-APPswe cells were stained with Lysotracker red and DAPI. Scale bar 5 μm. d Cathepsin B (CatB) activity was monitored in vitro from microsomal fractions of SH-APPswe cells treated with vehicle (V), D6, DAPT (DT) or NH 4 Cl <t>(NH4).</t> Data are represented as mean ± SEM, as determined by ANOVA one-way Dunnett post hoc test, *** p
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    Applichem nh4 cl
    γ-Secretase inhibitor treatment triggers autophagic dysfunction in APPswe-expressing SHSY-5Y but not mock-transfected cells. a SH-APPswe cells were treated with ELND006 (D6) and analyzed for APP-CTF levels by western blot. APP-CTFs were detected using α-APPct, 4G8 or 6E10 (see diagram for epitope recognition) and compared to C99-flag expression in HEK transfected cells. b Co-immunocytochemical staining of vehicle (Veh) or D6-treated APPswe cells using α-APPct and α-lamp1 shows a high overlap of these labelings. Scale bar 2 μm. c Vehicle (V) or D6-treated SH-mock or SH-APPswe cells were stained with Lysotracker red and DAPI. Scale bar 5 μm. d Cathepsin B (CatB) activity was monitored in vitro from microsomal fractions of SH-APPswe cells treated with vehicle (V), D6, DAPT (DT) or NH 4 Cl <t>(NH4).</t> Data are represented as mean ± SEM, as determined by ANOVA one-way Dunnett post hoc test, *** p
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    Image Search Results


    γ-Secretase inhibitor treatment triggers autophagic dysfunction in APPswe-expressing SHSY-5Y but not mock-transfected cells. a SH-APPswe cells were treated with ELND006 (D6) and analyzed for APP-CTF levels by western blot. APP-CTFs were detected using α-APPct, 4G8 or 6E10 (see diagram for epitope recognition) and compared to C99-flag expression in HEK transfected cells. b Co-immunocytochemical staining of vehicle (Veh) or D6-treated APPswe cells using α-APPct and α-lamp1 shows a high overlap of these labelings. Scale bar 2 μm. c Vehicle (V) or D6-treated SH-mock or SH-APPswe cells were stained with Lysotracker red and DAPI. Scale bar 5 μm. d Cathepsin B (CatB) activity was monitored in vitro from microsomal fractions of SH-APPswe cells treated with vehicle (V), D6, DAPT (DT) or NH 4 Cl (NH4). Data are represented as mean ± SEM, as determined by ANOVA one-way Dunnett post hoc test, *** p

    Journal: Acta Neuropathologica

    Article Title: Intraneuronal aggregation of the β-CTF fragment of APP (C99) induces Aβ-independent lysosomal-autophagic pathology

    doi: 10.1007/s00401-016-1577-6

    Figure Lengend Snippet: γ-Secretase inhibitor treatment triggers autophagic dysfunction in APPswe-expressing SHSY-5Y but not mock-transfected cells. a SH-APPswe cells were treated with ELND006 (D6) and analyzed for APP-CTF levels by western blot. APP-CTFs were detected using α-APPct, 4G8 or 6E10 (see diagram for epitope recognition) and compared to C99-flag expression in HEK transfected cells. b Co-immunocytochemical staining of vehicle (Veh) or D6-treated APPswe cells using α-APPct and α-lamp1 shows a high overlap of these labelings. Scale bar 2 μm. c Vehicle (V) or D6-treated SH-mock or SH-APPswe cells were stained with Lysotracker red and DAPI. Scale bar 5 μm. d Cathepsin B (CatB) activity was monitored in vitro from microsomal fractions of SH-APPswe cells treated with vehicle (V), D6, DAPT (DT) or NH 4 Cl (NH4). Data are represented as mean ± SEM, as determined by ANOVA one-way Dunnett post hoc test, *** p

    Article Snippet: Cell culture and experimental treatments The human neuroblastoma cell line SH-SY5Y, naive or stably expressing APPswe or pcDNA3 (mock), previously described [ ] was exposed to the following drugs for 16–20 h: D6 (50 nM to 5 μM, Elan Pharmaceuticals, San Francisco) in vehicle (methylcellulose/polysorbate 80, Sigma), DAPT (5 μM in DMSO, Sigma), leupeptin (10 μM in H2 O, Sigma), PADK (5 μM in DMSO, Bachem), NH4 Cl (10 mM in H2 O, Sigma), pepstatin A (10 μM in EtOH, Enzo Life sciences), smer28 (50 μM in DMSO, Sigma) and bafilomycin A1 (50 nM in H20, Sigma).

    Techniques: Expressing, Transfection, Western Blot, Staining, Activity Assay, In Vitro