Journal: The American Journal of Pathology
Article Title: Hepatitis C Virus Mimics Effects of Glypican-3 on CD81 and Promotes Development of Hepatocellular Carcinomas via Activation of Hippo Pathway in Hepatocytes
Figure Lengend Snippet: Forced expression of CD81 in glypican (Gpc)-3–expressing JM2 hepatoma cells results in activation of the Hippo pathway, a decrease in nuclear Yap, and a decrease in the percentage of bromodeoxyuridine (BrdU)-positive nuclei. JM2 cells were transiently transfected with pCMV6-CD81 plasmid, and after 12 hours, total cell lysates were collected for protein expression analysis. A: Expression of CD81, Gpc3, ezrin, and phosphorylated (p)-ezrin (Thr567). B: Cleaved (activated) p-mammalian STE20-like protein kinase (Mst)-1, yes-associated protein (Yap), and p-Yap. In both A and B , each band represents a separate cell culture. Results are representative of four experiments. C: JM2 cells in sparse density (50%) after 24 hours of transfection and 24 hours of BrdU incorporation. Positive ratio indicates the fraction of cells whose nuclei were positive for BrdU by IHC analysis. Data are expressed as means ± SEM, derived from four separate cultures for each category ( C ). P = 0.0078.
Article Snippet: Signaling functions of CD81 were first demonstrated by treatment with the specific agonistic antibody 5A6.
Techniques: Expressing, Gel Permeation Chromatography, Activation Assay, Transfection, Plasmid Preparation, Microscale Thermophoresis, Cell Culture, BrdU Incorporation Assay, Immunohistochemistry, Derivative Assay