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  • 99
    Thermo Fisher aqueous mounting medium
    Aqueous Mounting Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 390 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore anti myoferlin
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    Ventana Medical nexes stainer
    Nexes Stainer, supplied by Ventana Medical, used in various techniques. Bioz Stars score: 85/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Ventana Medical nexes automated stainer
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    Ventana Medical nex es automated stainer
    Nex Es Automated Stainer, supplied by Ventana Medical, used in various techniques. Bioz Stars score: 85/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Ventana Medical cc1 antigen retrieval buffer
    Cc1 Antigen Retrieval Buffer, supplied by Ventana Medical, used in various techniques. Bioz Stars score: 88/100, based on 125 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Ventana Medical hydrogen peroxide
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    Ventana Medical immunohistochemistry ihc
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    Ventana Medical immunohistochemistry immunohistochemistry
    Overexpression of IGF2BP3 predicts poor prognosis in GC. a Over-expressed IGF2BP3 was related to worse overall outcome and significantly associated with first progression survival in primary GC samples from Kaplan Meier plotter. b In TCGA cohort, high IGF2BP3 expression showed a non-significance trend to predict unfavorable overall and recurrence free survival. c Left panel , representative <t>immunohistochemistry</t> images of IGF2BP3 in normal gastric epithelium tissues, intestinal-, diffuse type GC samples (original magnification × 100, insertion × 400). IGF2BP3 expression was mainly localized in the cytoplasm. Right panel, Kaplan-Meier plots of disease specific survival according to IGF2BP3 expression status. IGF2BP3 accumulation in cytoplasm (moderate/strong staining) was associated with poor disease specific survival in patients with GC ( P = 0.012)
    Immunohistochemistry Immunohistochemistry, supplied by Ventana Medical, used in various techniques. Bioz Stars score: 93/100, based on 1424 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Ventana Medical detection kit
    Overexpression of IGF2BP3 predicts poor prognosis in GC. a Over-expressed IGF2BP3 was related to worse overall outcome and significantly associated with first progression survival in primary GC samples from Kaplan Meier plotter. b In TCGA cohort, high IGF2BP3 expression showed a non-significance trend to predict unfavorable overall and recurrence free survival. c Left panel , representative <t>immunohistochemistry</t> images of IGF2BP3 in normal gastric epithelium tissues, intestinal-, diffuse type GC samples (original magnification × 100, insertion × 400). IGF2BP3 expression was mainly localized in the cytoplasm. Right panel, Kaplan-Meier plots of disease specific survival according to IGF2BP3 expression status. IGF2BP3 accumulation in cytoplasm (moderate/strong staining) was associated with poor disease specific survival in patients with GC ( P = 0.012)
    Detection Kit, supplied by Ventana Medical, used in various techniques. Bioz Stars score: 92/100, based on 415 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Ventana Medical endogenous peroxidase
    Overexpression of IGF2BP3 predicts poor prognosis in GC. a Over-expressed IGF2BP3 was related to worse overall outcome and significantly associated with first progression survival in primary GC samples from Kaplan Meier plotter. b In TCGA cohort, high IGF2BP3 expression showed a non-significance trend to predict unfavorable overall and recurrence free survival. c Left panel , representative <t>immunohistochemistry</t> images of IGF2BP3 in normal gastric epithelium tissues, intestinal-, diffuse type GC samples (original magnification × 100, insertion × 400). IGF2BP3 expression was mainly localized in the cytoplasm. Right panel, Kaplan-Meier plots of disease specific survival according to IGF2BP3 expression status. IGF2BP3 accumulation in cytoplasm (moderate/strong staining) was associated with poor disease specific survival in patients with GC ( P = 0.012)
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    Ventana Medical erbb3 required retrieval
    MM-121 specifically downregulates Survivin and significantly enhances paclitaxel-induced anti-proliferative/anti-survival effects and apoptosis in a trastuzumab-resistant breast cancer cell line. (A) BT474-HR20 cells were untreated, or treated with MM-121 (10 μg/ml) for 24 or 48 h. Cells were collected and subjected to western blot analyses of <t>P-erbB3,</t> erbB3, Survivin, Bcl-xL, Mcl-1, or β-actin. The densitometry analyses of Survivin signals are shown underneath, and the arbitrary numbers indicate the intensities of each sample relative to control, defined as 1.0. ( B) BT474-HR20 cells were plated onto 96-well plates and incubated at 37°C with 5% CO 2 . After 24 h, the culture medium was replaced with 0.1 ml fresh medium containing 0.5% FBS or the same medium containing the indicated concentrations of paclitaxel in the absence (paclitaxel) or presence (MM-121 + pac) of MM-121 (10 μg/ml) for another 72 h. The percentages of surviving cells from each cell line relative to controls, defined as 100% survival, were determined by reduction of 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt (MTS). Bars represent SD. Data are representative of three independent experiments. (C and D) BT474-HR20 cells were untreated, or treated with either MM-121 (10 μg/ml) or paclitaxel (8 nmol/L) alone, or their combinations for 24 h. Cells were collected and subjected to western blot analyses of poly(ADP-ribose) polymerase (PARP), caspase-8, caspase-3, or β-actin (C) ; or a specific apoptosis ELISA (D) . Bars represent SD. * P
    Erbb3 Required Retrieval, supplied by Ventana Medical, used in various techniques. Bioz Stars score: 85/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Spring Bioscience cd40
    <t>CD40</t> is expressed on TAMs of cancer patients. (A) Exemplary IHC images for CD163 and CSF-1R staining and duplex staining for CD68/CD40 staining of one exemplary CRC and one exemplary mesothelioma patient. Black arrowheads indicate examples of CD68 + CD40 + double-positive macrophages. (B) Overall correlation shown as heat maps of 9 CRC and 19 mesothelioma patients using data obtained by automated digital analyses. (C) CD68 + CD40 + double-positive cells in CRC and mesothelioma patients quantified as cell counts per squared millimeters of tumor tissue by digital analyses. (D) CSF-1R + macrophages and CD3 + T cells colocalize in CRC and mesothelioma as assessed from consecutive sections.
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    Ventana Medical ki67
    The combination of MM-121 and trastuzumab significantly inhibits proliferation and induces apoptosis of trastuzumab-resistant BT474-HR20 breast cancer cells in vivo . The tumors obtained from the animal studies described above were evaluated by IHC analysis of <t>Ki67</t> and cleaved caspase-3. A , Data show the representative images of the immunostaining of Ki67 and cleaved caspase-3 (Cleaved Casp-3). B , The IHC slides were observed by two independent personnel. The tumor cells with positive staining of Ki67 or cleaved caspase-3 were counted from three randomly selected areas in each slide. The three areas were first identified by scanning the entire slide at ×10 magnification, and then the positive staining cells were counted at ×20 magnification using an Olympus BX40 Microscope. The bar graphs show the average of positive staining cells in each field. Bars , SD. The combinatorial treated mice had significantly fewer cells stained positive for Ki67 and more cells stained positive for cleaved caspase-3 than control mice or single Ab treated mice, P
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    Roper Technologies acquisition software image pro plus version 5 1
    The combination of MM-121 and trastuzumab significantly inhibits proliferation and induces apoptosis of trastuzumab-resistant BT474-HR20 breast cancer cells in vivo . The tumors obtained from the animal studies described above were evaluated by IHC analysis of <t>Ki67</t> and cleaved caspase-3. A , Data show the representative images of the immunostaining of Ki67 and cleaved caspase-3 (Cleaved Casp-3). B , The IHC slides were observed by two independent personnel. The tumor cells with positive staining of Ki67 or cleaved caspase-3 were counted from three randomly selected areas in each slide. The three areas were first identified by scanning the entire slide at ×10 magnification, and then the positive staining cells were counted at ×20 magnification using an Olympus BX40 Microscope. The bar graphs show the average of positive staining cells in each field. Bars , SD. The combinatorial treated mice had significantly fewer cells stained positive for Ki67 and more cells stained positive for cleaved caspase-3 than control mice or single Ab treated mice, P
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    88
    Ventana Medical automated slide stainer
    The combination of MM-121 and trastuzumab significantly inhibits proliferation and induces apoptosis of trastuzumab-resistant BT474-HR20 breast cancer cells in vivo . The tumors obtained from the animal studies described above were evaluated by IHC analysis of <t>Ki67</t> and cleaved caspase-3. A , Data show the representative images of the immunostaining of Ki67 and cleaved caspase-3 (Cleaved Casp-3). B , The IHC slides were observed by two independent personnel. The tumor cells with positive staining of Ki67 or cleaved caspase-3 were counted from three randomly selected areas in each slide. The three areas were first identified by scanning the entire slide at ×10 magnification, and then the positive staining cells were counted at ×20 magnification using an Olympus BX40 Microscope. The bar graphs show the average of positive staining cells in each field. Bars , SD. The combinatorial treated mice had significantly fewer cells stained positive for Ki67 and more cells stained positive for cleaved caspase-3 than control mice or single Ab treated mice, P
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    92
    Agilent technologies anti desmin
    The combination of MM-121 and trastuzumab significantly inhibits proliferation and induces apoptosis of trastuzumab-resistant BT474-HR20 breast cancer cells in vivo . The tumors obtained from the animal studies described above were evaluated by IHC analysis of <t>Ki67</t> and cleaved caspase-3. A , Data show the representative images of the immunostaining of Ki67 and cleaved caspase-3 (Cleaved Casp-3). B , The IHC slides were observed by two independent personnel. The tumor cells with positive staining of Ki67 or cleaved caspase-3 were counted from three randomly selected areas in each slide. The three areas were first identified by scanning the entire slide at ×10 magnification, and then the positive staining cells were counted at ×20 magnification using an Olympus BX40 Microscope. The bar graphs show the average of positive staining cells in each field. Bars , SD. The combinatorial treated mice had significantly fewer cells stained positive for Ki67 and more cells stained positive for cleaved caspase-3 than control mice or single Ab treated mice, P
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    Cell Signaling Technology Inc anti transforming growth factor beta induced
    The combination of MM-121 and trastuzumab significantly inhibits proliferation and induces apoptosis of trastuzumab-resistant BT474-HR20 breast cancer cells in vivo . The tumors obtained from the animal studies described above were evaluated by IHC analysis of <t>Ki67</t> and cleaved caspase-3. A , Data show the representative images of the immunostaining of Ki67 and cleaved caspase-3 (Cleaved Casp-3). B , The IHC slides were observed by two independent personnel. The tumor cells with positive staining of Ki67 or cleaved caspase-3 were counted from three randomly selected areas in each slide. The three areas were first identified by scanning the entire slide at ×10 magnification, and then the positive staining cells were counted at ×20 magnification using an Olympus BX40 Microscope. The bar graphs show the average of positive staining cells in each field. Bars , SD. The combinatorial treated mice had significantly fewer cells stained positive for Ki67 and more cells stained positive for cleaved caspase-3 than control mice or single Ab treated mice, P
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    Novocastra anti cd56
    The combination of MM-121 and trastuzumab significantly inhibits proliferation and induces apoptosis of trastuzumab-resistant BT474-HR20 breast cancer cells in vivo . The tumors obtained from the animal studies described above were evaluated by IHC analysis of <t>Ki67</t> and cleaved caspase-3. A , Data show the representative images of the immunostaining of Ki67 and cleaved caspase-3 (Cleaved Casp-3). B , The IHC slides were observed by two independent personnel. The tumor cells with positive staining of Ki67 or cleaved caspase-3 were counted from three randomly selected areas in each slide. The three areas were first identified by scanning the entire slide at ×10 magnification, and then the positive staining cells were counted at ×20 magnification using an Olympus BX40 Microscope. The bar graphs show the average of positive staining cells in each field. Bars , SD. The combinatorial treated mice had significantly fewer cells stained positive for Ki67 and more cells stained positive for cleaved caspase-3 than control mice or single Ab treated mice, P
    Anti Cd56, supplied by Novocastra, used in various techniques. Bioz Stars score: 92/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies anti ki67
    Effect of HDAC and COX-2 co-inhibition on BxPC-3 tumor growth on CAM. (A) Macroscopic pictures were obtained at the same magnification from bottom and side view. (B) Tumor volume at day 7 after cell implantation. Tumors were treated with 30 µl celecoxib (8 µM), MS-275 (0.2 µM) or drug combination at same concentration. (C) Western-blot detection of COX-2 in 20 µg proteins isolated from tumors grown on CAM and treated with MS-275 (0.2 µM). HSC70 was used as a loading control. (D) Histological aspect of tumors grown on CAM during 7 days and treated with 30 µl celecoxib (8 µM), MS-275 (0.2 µM) or drug combination at same concentration. (E) Western-blot detection of caspase-3 in 40 µg proteins isolated from tumors grown on CAM and treated with MS-275 (0.2 µM) or celecoxib (8 µM). HSC70 was used as a loading control. (F) <t>Ki67</t> immunostaining and associated quantification of tumors grown on CAM during 7 days and treated with 30 µl celecoxib (8 µM), MS-275 (0.2 µM) or drug combination at same concentration. Results are expressed as mean ± s.d. ***P
    Anti Ki67, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 1617 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology anti latent transforming growth factor beta binding protein 2
    Effect of HDAC and COX-2 co-inhibition on BxPC-3 tumor growth on CAM. (A) Macroscopic pictures were obtained at the same magnification from bottom and side view. (B) Tumor volume at day 7 after cell implantation. Tumors were treated with 30 µl celecoxib (8 µM), MS-275 (0.2 µM) or drug combination at same concentration. (C) Western-blot detection of COX-2 in 20 µg proteins isolated from tumors grown on CAM and treated with MS-275 (0.2 µM). HSC70 was used as a loading control. (D) Histological aspect of tumors grown on CAM during 7 days and treated with 30 µl celecoxib (8 µM), MS-275 (0.2 µM) or drug combination at same concentration. (E) Western-blot detection of caspase-3 in 40 µg proteins isolated from tumors grown on CAM and treated with MS-275 (0.2 µM) or celecoxib (8 µM). HSC70 was used as a loading control. (F) <t>Ki67</t> immunostaining and associated quantification of tumors grown on CAM during 7 days and treated with 30 µl celecoxib (8 µM), MS-275 (0.2 µM) or drug combination at same concentration. Results are expressed as mean ± s.d. ***P
    Anti Latent Transforming Growth Factor Beta Binding Protein 2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Overexpression of IGF2BP3 predicts poor prognosis in GC. a Over-expressed IGF2BP3 was related to worse overall outcome and significantly associated with first progression survival in primary GC samples from Kaplan Meier plotter. b In TCGA cohort, high IGF2BP3 expression showed a non-significance trend to predict unfavorable overall and recurrence free survival. c Left panel , representative immunohistochemistry images of IGF2BP3 in normal gastric epithelium tissues, intestinal-, diffuse type GC samples (original magnification × 100, insertion × 400). IGF2BP3 expression was mainly localized in the cytoplasm. Right panel, Kaplan-Meier plots of disease specific survival according to IGF2BP3 expression status. IGF2BP3 accumulation in cytoplasm (moderate/strong staining) was associated with poor disease specific survival in patients with GC ( P = 0.012)

    Journal: Molecular Cancer

    Article Title: IGF2BP3 functions as a potential oncogene and is a crucial target of miR-34a in gastric carcinogenesis

    doi: 10.1186/s12943-017-0647-2

    Figure Lengend Snippet: Overexpression of IGF2BP3 predicts poor prognosis in GC. a Over-expressed IGF2BP3 was related to worse overall outcome and significantly associated with first progression survival in primary GC samples from Kaplan Meier plotter. b In TCGA cohort, high IGF2BP3 expression showed a non-significance trend to predict unfavorable overall and recurrence free survival. c Left panel , representative immunohistochemistry images of IGF2BP3 in normal gastric epithelium tissues, intestinal-, diffuse type GC samples (original magnification × 100, insertion × 400). IGF2BP3 expression was mainly localized in the cytoplasm. Right panel, Kaplan-Meier plots of disease specific survival according to IGF2BP3 expression status. IGF2BP3 accumulation in cytoplasm (moderate/strong staining) was associated with poor disease specific survival in patients with GC ( P = 0.012)

    Article Snippet: Immunohistochemistry Immunohistochemistry was to conduct tissue microarray within a 4 μm-thick section of each clinical sample using Ventana NexES automated Stainer (Ventana Corporation).

    Techniques: Over Expression, Expressing, Immunohistochemistry, Staining

    MM-121 specifically downregulates Survivin and significantly enhances paclitaxel-induced anti-proliferative/anti-survival effects and apoptosis in a trastuzumab-resistant breast cancer cell line. (A) BT474-HR20 cells were untreated, or treated with MM-121 (10 μg/ml) for 24 or 48 h. Cells were collected and subjected to western blot analyses of P-erbB3, erbB3, Survivin, Bcl-xL, Mcl-1, or β-actin. The densitometry analyses of Survivin signals are shown underneath, and the arbitrary numbers indicate the intensities of each sample relative to control, defined as 1.0. ( B) BT474-HR20 cells were plated onto 96-well plates and incubated at 37°C with 5% CO 2 . After 24 h, the culture medium was replaced with 0.1 ml fresh medium containing 0.5% FBS or the same medium containing the indicated concentrations of paclitaxel in the absence (paclitaxel) or presence (MM-121 + pac) of MM-121 (10 μg/ml) for another 72 h. The percentages of surviving cells from each cell line relative to controls, defined as 100% survival, were determined by reduction of 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt (MTS). Bars represent SD. Data are representative of three independent experiments. (C and D) BT474-HR20 cells were untreated, or treated with either MM-121 (10 μg/ml) or paclitaxel (8 nmol/L) alone, or their combinations for 24 h. Cells were collected and subjected to western blot analyses of poly(ADP-ribose) polymerase (PARP), caspase-8, caspase-3, or β-actin (C) ; or a specific apoptosis ELISA (D) . Bars represent SD. * P

    Journal: Breast Cancer Research : BCR

    Article Title: Therapeutic targeting of erbB3 with MM-121/SAR256212 enhances antitumor activity of paclitaxel against erbB2-overexpressing breast cancer

    doi: 10.1186/bcr3563

    Figure Lengend Snippet: MM-121 specifically downregulates Survivin and significantly enhances paclitaxel-induced anti-proliferative/anti-survival effects and apoptosis in a trastuzumab-resistant breast cancer cell line. (A) BT474-HR20 cells were untreated, or treated with MM-121 (10 μg/ml) for 24 or 48 h. Cells were collected and subjected to western blot analyses of P-erbB3, erbB3, Survivin, Bcl-xL, Mcl-1, or β-actin. The densitometry analyses of Survivin signals are shown underneath, and the arbitrary numbers indicate the intensities of each sample relative to control, defined as 1.0. ( B) BT474-HR20 cells were plated onto 96-well plates and incubated at 37°C with 5% CO 2 . After 24 h, the culture medium was replaced with 0.1 ml fresh medium containing 0.5% FBS or the same medium containing the indicated concentrations of paclitaxel in the absence (paclitaxel) or presence (MM-121 + pac) of MM-121 (10 μg/ml) for another 72 h. The percentages of surviving cells from each cell line relative to controls, defined as 100% survival, were determined by reduction of 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt (MTS). Bars represent SD. Data are representative of three independent experiments. (C and D) BT474-HR20 cells were untreated, or treated with either MM-121 (10 μg/ml) or paclitaxel (8 nmol/L) alone, or their combinations for 24 h. Cells were collected and subjected to western blot analyses of poly(ADP-ribose) polymerase (PARP), caspase-8, caspase-3, or β-actin (C) ; or a specific apoptosis ELISA (D) . Bars represent SD. * P

    Article Snippet: ErbB3 required retrieval in Cell Conditioner 1 (standard retrieval time, Ventana Medical Systems, Tucson, AZ, USA).

    Techniques: Western Blot, Incubation, Enzyme-linked Immunosorbent Assay

    MM-121 significantly enhances paclitaxel-mediated anti-proliferative/anti-survival effects on breast cancer cell lines with expression of both erbB2 and erbB3. (A) SKBR3.neo1, SKBR3.B3.1, or SKBR3.B3.2 cells were plated onto 96-well plates and incubated at 37°C with 5% CO 2 . After 24 h, the culture medium was replaced with 0.1 ml fresh medium containing 0.5% FBS or the same medium containing the indicated concentrations of paclitaxel in the absence (paclitaxel) or presence (MM-121 + pac) of MM-121 (10 μg/ml) for another 72 h. The percentages of surviving cells from each cell line relative to controls, defined as 100% survival, were determined by reduction of 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt (MTS). Bars represent SD. Data are representative of three independent experiments. (B) The same cells were untreated or treated with MM-121 (10 μg/ml) for 24 or 48 h. Cells were collected and subjected to western blot analyses of phosphorylated (P)-erbB2, erbB2, P-erbB3, erbB3, P-Akt, Akt, P-mitogen-activated protein kinase (P-MAPK), MAPK, or β-actin.

    Journal: Breast Cancer Research : BCR

    Article Title: Therapeutic targeting of erbB3 with MM-121/SAR256212 enhances antitumor activity of paclitaxel against erbB2-overexpressing breast cancer

    doi: 10.1186/bcr3563

    Figure Lengend Snippet: MM-121 significantly enhances paclitaxel-mediated anti-proliferative/anti-survival effects on breast cancer cell lines with expression of both erbB2 and erbB3. (A) SKBR3.neo1, SKBR3.B3.1, or SKBR3.B3.2 cells were plated onto 96-well plates and incubated at 37°C with 5% CO 2 . After 24 h, the culture medium was replaced with 0.1 ml fresh medium containing 0.5% FBS or the same medium containing the indicated concentrations of paclitaxel in the absence (paclitaxel) or presence (MM-121 + pac) of MM-121 (10 μg/ml) for another 72 h. The percentages of surviving cells from each cell line relative to controls, defined as 100% survival, were determined by reduction of 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt (MTS). Bars represent SD. Data are representative of three independent experiments. (B) The same cells were untreated or treated with MM-121 (10 μg/ml) for 24 or 48 h. Cells were collected and subjected to western blot analyses of phosphorylated (P)-erbB2, erbB2, P-erbB3, erbB3, P-Akt, Akt, P-mitogen-activated protein kinase (P-MAPK), MAPK, or β-actin.

    Article Snippet: ErbB3 required retrieval in Cell Conditioner 1 (standard retrieval time, Ventana Medical Systems, Tucson, AZ, USA).

    Techniques: Expressing, Incubation, Western Blot

    CD40 is expressed on TAMs of cancer patients. (A) Exemplary IHC images for CD163 and CSF-1R staining and duplex staining for CD68/CD40 staining of one exemplary CRC and one exemplary mesothelioma patient. Black arrowheads indicate examples of CD68 + CD40 + double-positive macrophages. (B) Overall correlation shown as heat maps of 9 CRC and 19 mesothelioma patients using data obtained by automated digital analyses. (C) CD68 + CD40 + double-positive cells in CRC and mesothelioma patients quantified as cell counts per squared millimeters of tumor tissue by digital analyses. (D) CSF-1R + macrophages and CD3 + T cells colocalize in CRC and mesothelioma as assessed from consecutive sections.

    Journal: The Journal of Experimental Medicine

    Article Title: Rapid activation of tumor-associated macrophages boosts preexisting tumor immunity

    doi: 10.1084/jem.20171440

    Figure Lengend Snippet: CD40 is expressed on TAMs of cancer patients. (A) Exemplary IHC images for CD163 and CSF-1R staining and duplex staining for CD68/CD40 staining of one exemplary CRC and one exemplary mesothelioma patient. Black arrowheads indicate examples of CD68 + CD40 + double-positive macrophages. (B) Overall correlation shown as heat maps of 9 CRC and 19 mesothelioma patients using data obtained by automated digital analyses. (C) CD68 + CD40 + double-positive cells in CRC and mesothelioma patients quantified as cell counts per squared millimeters of tumor tissue by digital analyses. (D) CSF-1R + macrophages and CD3 + T cells colocalize in CRC and mesothelioma as assessed from consecutive sections.

    Article Snippet: All reagents except the CD40 (E3704; Spring Bioscience) and CSF-1R (clone 29; Roche) antibodies were obtained from Ventana Medical Systems.

    Techniques: Immunohistochemistry, Staining

    The combination of MM-121 and trastuzumab significantly inhibits proliferation and induces apoptosis of trastuzumab-resistant BT474-HR20 breast cancer cells in vivo . The tumors obtained from the animal studies described above were evaluated by IHC analysis of Ki67 and cleaved caspase-3. A , Data show the representative images of the immunostaining of Ki67 and cleaved caspase-3 (Cleaved Casp-3). B , The IHC slides were observed by two independent personnel. The tumor cells with positive staining of Ki67 or cleaved caspase-3 were counted from three randomly selected areas in each slide. The three areas were first identified by scanning the entire slide at ×10 magnification, and then the positive staining cells were counted at ×20 magnification using an Olympus BX40 Microscope. The bar graphs show the average of positive staining cells in each field. Bars , SD. The combinatorial treated mice had significantly fewer cells stained positive for Ki67 and more cells stained positive for cleaved caspase-3 than control mice or single Ab treated mice, P

    Journal: Molecular Cancer

    Article Title: The anti-erbB3 antibody MM-121/SAR256212 in combination with trastuzumab exerts potent antitumor activity against trastuzumab-resistant breast cancer cells

    doi: 10.1186/1476-4598-12-134

    Figure Lengend Snippet: The combination of MM-121 and trastuzumab significantly inhibits proliferation and induces apoptosis of trastuzumab-resistant BT474-HR20 breast cancer cells in vivo . The tumors obtained from the animal studies described above were evaluated by IHC analysis of Ki67 and cleaved caspase-3. A , Data show the representative images of the immunostaining of Ki67 and cleaved caspase-3 (Cleaved Casp-3). B , The IHC slides were observed by two independent personnel. The tumor cells with positive staining of Ki67 or cleaved caspase-3 were counted from three randomly selected areas in each slide. The three areas were first identified by scanning the entire slide at ×10 magnification, and then the positive staining cells were counted at ×20 magnification using an Olympus BX40 Microscope. The bar graphs show the average of positive staining cells in each field. Bars , SD. The combinatorial treated mice had significantly fewer cells stained positive for Ki67 and more cells stained positive for cleaved caspase-3 than control mice or single Ab treated mice, P

    Article Snippet: Ki67 and cleaved caspase-3 antibodies were incubated for 32 min and detected with a modified I-VIEW DAB (Ventana) detection kit.

    Techniques: In Vivo, Immunohistochemistry, Immunostaining, Staining, Microscopy, Mouse Assay

    Effect of HDAC and COX-2 co-inhibition on BxPC-3 tumor growth on CAM. (A) Macroscopic pictures were obtained at the same magnification from bottom and side view. (B) Tumor volume at day 7 after cell implantation. Tumors were treated with 30 µl celecoxib (8 µM), MS-275 (0.2 µM) or drug combination at same concentration. (C) Western-blot detection of COX-2 in 20 µg proteins isolated from tumors grown on CAM and treated with MS-275 (0.2 µM). HSC70 was used as a loading control. (D) Histological aspect of tumors grown on CAM during 7 days and treated with 30 µl celecoxib (8 µM), MS-275 (0.2 µM) or drug combination at same concentration. (E) Western-blot detection of caspase-3 in 40 µg proteins isolated from tumors grown on CAM and treated with MS-275 (0.2 µM) or celecoxib (8 µM). HSC70 was used as a loading control. (F) Ki67 immunostaining and associated quantification of tumors grown on CAM during 7 days and treated with 30 µl celecoxib (8 µM), MS-275 (0.2 µM) or drug combination at same concentration. Results are expressed as mean ± s.d. ***P

    Journal: PLoS ONE

    Article Title: The Anti-Tumor Effect of HDAC Inhibition in a Human Pancreas Cancer Model Is Significantly Improved by the Simultaneous Inhibition of Cyclooxygenase 2

    doi: 10.1371/journal.pone.0075102

    Figure Lengend Snippet: Effect of HDAC and COX-2 co-inhibition on BxPC-3 tumor growth on CAM. (A) Macroscopic pictures were obtained at the same magnification from bottom and side view. (B) Tumor volume at day 7 after cell implantation. Tumors were treated with 30 µl celecoxib (8 µM), MS-275 (0.2 µM) or drug combination at same concentration. (C) Western-blot detection of COX-2 in 20 µg proteins isolated from tumors grown on CAM and treated with MS-275 (0.2 µM). HSC70 was used as a loading control. (D) Histological aspect of tumors grown on CAM during 7 days and treated with 30 µl celecoxib (8 µM), MS-275 (0.2 µM) or drug combination at same concentration. (E) Western-blot detection of caspase-3 in 40 µg proteins isolated from tumors grown on CAM and treated with MS-275 (0.2 µM) or celecoxib (8 µM). HSC70 was used as a loading control. (F) Ki67 immunostaining and associated quantification of tumors grown on CAM during 7 days and treated with 30 µl celecoxib (8 µM), MS-275 (0.2 µM) or drug combination at same concentration. Results are expressed as mean ± s.d. ***P

    Article Snippet: Following antibodies were used: anti-cytokeratin 7 (CK7 - Dako, Glostrup, Denmark), anti-cytokeratin 19 (CK19 - Roche Diagnostics, Vilvoorde, Belgium), anti-cytokeratin 20 (CK20 - Dako, Glostrup, Denmark), anti-CD56 (Novocastra, Leica Microsystem Inc, Buffalo Grove, IL), anti-carcinoembryonic antigen (CEA - Roche Diagnostics, Vilvoorde, Belgium), anti-Ki67 (Dako, Glostrup, Denmark), anti-latent transforming growth factor-beta binding protein 2 (LTBP2 – Santa Cruz Biotchnology, Santa Cruz, CA), anti-transforming growth factor beta-induced (TGFBI - Cell Signalling, Danvers, MA), anti-myoferlin (Sigma, Bornem, Belgium) and anti-desmin (Dako, Glostrup, Denmark) were used for the primary reaction.

    Techniques: Inhibition, Chick Chorioallantoic Membrane Assay, Mass Spectrometry, Concentration Assay, Western Blot, Isolation, Immunostaining