neun Search Results


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    Synonyms Fox 3 Rbfox 3 Fox3 Price 300 00 Host Guinea pig Immunogen Recombinant protein corresponding to AA 1 to 97 from mouse NeuN UniProt Id Q8BIF2 Reactivity rat mouse
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    99
    Millipore mouse anti neun
    Lack of colocalization of P2ry13 mRNA with other cell types of the DG (young adults). (A–E) Details of DG showing double labeling using FISH for P2ry13 (red) and immunocytochemistry for various cell markers (green); overlay (left), immunolabeling (right; all WT animals). (A) Glial fibrillary acidic protein (GFAP), (B) <t>Sox2,</t> (C) DCX (weak immunolabeling due to experimental conditions required for FISH) (D) <t>NeuN.</t> (E) Triple labeling for somatostatin (green, St), parvalbumin (white PV) and for P2ry13 (red). The GCL is outlined with dashed lines. Arrows, cell bodies labeled for P2ry13 mRNA; arrow head, P2ry13 -negative astrocyte. (F) Simplified schematic of principal cell types along the hippocampal neurogenesis pathway. Lineage markers used in this study and their cellular location are indicated by bars. gcl, granule cell layer; mol, molecular layer; sgz, subgranular zone. Scale bars, 25 μm.
    Mouse Anti Neun, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 12574 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore neun
    Lack of colocalization of P2ry13 mRNA with other cell types of the DG (young adults). (A–E) Details of DG showing double labeling using FISH for P2ry13 (red) and immunocytochemistry for various cell markers (green); overlay (left), immunolabeling (right; all WT animals). (A) Glial fibrillary acidic protein (GFAP), (B) <t>Sox2,</t> (C) DCX (weak immunolabeling due to experimental conditions required for FISH) (D) <t>NeuN.</t> (E) Triple labeling for somatostatin (green, St), parvalbumin (white PV) and for P2ry13 (red). The GCL is outlined with dashed lines. Arrows, cell bodies labeled for P2ry13 mRNA; arrow head, P2ry13 -negative astrocyte. (F) Simplified schematic of principal cell types along the hippocampal neurogenesis pathway. Lineage markers used in this study and their cellular location are indicated by bars. gcl, granule cell layer; mol, molecular layer; sgz, subgranular zone. Scale bars, 25 μm.
    Neun, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 11856 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam anti neun
    Exercise enhances proliferation of neurons in the hippocampal dentate gyrus. (A) Immunofluorescence staining for <t>BrdU</t> (red) and <t>NeuN</t> (green) in rats 21 days after MCAO. Scale bar=50 µm. (B) Quantification of BrdU + /NeuN + cells 7, 14 and 21 days after MCAO. Data are presented as the means ± standard deviation. The arrows in the images are indicating BrdU + /NeuN + cells. *P
    Anti Neun, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 756 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    neun  (Abcam)
    99
    Abcam neun
    Exercise enhances proliferation of neurons in the hippocampal dentate gyrus. (A) Immunofluorescence staining for <t>BrdU</t> (red) and <t>NeuN</t> (green) in rats 21 days after MCAO. Scale bar=50 µm. (B) Quantification of BrdU + /NeuN + cells 7, 14 and 21 days after MCAO. Data are presented as the means ± standard deviation. The arrows in the images are indicating BrdU + /NeuN + cells. *P
    Neun, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1317 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam mouse anti neun
    Exercise enhances proliferation of neurons in the hippocampal dentate gyrus. (A) Immunofluorescence staining for <t>BrdU</t> (red) and <t>NeuN</t> (green) in rats 21 days after MCAO. Scale bar=50 µm. (B) Quantification of BrdU + /NeuN + cells 7, 14 and 21 days after MCAO. Data are presented as the means ± standard deviation. The arrows in the images are indicating BrdU + /NeuN + cells. *P
    Mouse Anti Neun, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 571 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA mouse anti neun
    Analysis of transduced neurons after i Mecp2 gene transfer in Mecp2 -/y brains. ( a ) Characterization of i Mecp2 -transduced cells in KO cortex (1×10 9 , 1×10 10 , 1×10 11 ,1×10 12 vg/mouse) using colocalization of <t>V5</t> + cells with a neuronal marker <t>(NeuN).</t> ( b ) Quantification of V5 + cells on total DAPI + cells (orange bar) and V5 + /NeuN + cells on total DAPI + cells (green bar). Dashed line indicates the number of NeuN + cells on total DAPI + cells. ( c–d ) Colocalization and quantification of transduced (V5+) GABAerigic interneurons (marked with GABA, GABA + ) in i Mecp2 -transduced KO cortexes of animals treated with a 1×10 11 vg/mouse dose. Nuclei were stained with DAPI. All images were captured using confocal microscope. Scale bar: 50 μm. n = 3 animals per group.
    Mouse Anti Neun, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 278 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Millipore monoclonal anti neun
    Analysis of transduced neurons after i Mecp2 gene transfer in Mecp2 -/y brains. ( a ) Characterization of i Mecp2 -transduced cells in KO cortex (1×10 9 , 1×10 10 , 1×10 11 ,1×10 12 vg/mouse) using colocalization of <t>V5</t> + cells with a neuronal marker <t>(NeuN).</t> ( b ) Quantification of V5 + cells on total DAPI + cells (orange bar) and V5 + /NeuN + cells on total DAPI + cells (green bar). Dashed line indicates the number of NeuN + cells on total DAPI + cells. ( c–d ) Colocalization and quantification of transduced (V5+) GABAerigic interneurons (marked with GABA, GABA + ) in i Mecp2 -transduced KO cortexes of animals treated with a 1×10 11 vg/mouse dose. Nuclei were stained with DAPI. All images were captured using confocal microscope. Scale bar: 50 μm. n = 3 animals per group.
    Monoclonal Anti Neun, supplied by Millipore, used in various techniques. Bioz Stars score: 89/100, based on 247 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Abcam anti neun antibody neuronal marker
    Analysis of transduced neurons after i Mecp2 gene transfer in Mecp2 -/y brains. ( a ) Characterization of i Mecp2 -transduced cells in KO cortex (1×10 9 , 1×10 10 , 1×10 11 ,1×10 12 vg/mouse) using colocalization of <t>V5</t> + cells with a neuronal marker <t>(NeuN).</t> ( b ) Quantification of V5 + cells on total DAPI + cells (orange bar) and V5 + /NeuN + cells on total DAPI + cells (green bar). Dashed line indicates the number of NeuN + cells on total DAPI + cells. ( c–d ) Colocalization and quantification of transduced (V5+) GABAerigic interneurons (marked with GABA, GABA + ) in i Mecp2 -transduced KO cortexes of animals treated with a 1×10 11 vg/mouse dose. Nuclei were stained with DAPI. All images were captured using confocal microscope. Scale bar: 50 μm. n = 3 animals per group.
    Anti Neun Antibody Neuronal Marker, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA anti neun
    Angiotensin receptor type 2 is involved in heat acclimation (HA)-mediated enhanced neurogenesis in the subventricular zone (SVZ). Mice were subjected to traumatic brain injury (TBI) after treatment with saline or PD123319 (PD, 10 mg/kg/day) for 3 days, and killed 42 days post TBI. Evenly sliced 10- μ m sections were incubated with <t>anti-NeuN</t> for mature neurons and with <t>anti-BrdU</t> (anti-5-bromo-2-deoxyuridine) for newborn cells. ( A ) HA induced neurogenesis in the SVZ (i, j) and PD123319 (PD, 10 mg/kg/day) treatment blocked the observed neurogenesis (m–p). PD treatment (e–h) did not reduce neurogenesis in normothermic (NT) mice measured after saline treatment (a–d). Stereological quantification of the fields was used to count the number of BrdU-positive cells (BrdU + ). Results show the average number of BrdU + per field ( B ), and the average number of double-positive cells for NeuN and for BrdU (BrdU + /NeuN + ) per field ( C ). * P
    Anti Neun, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 260 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore neuron specific nuclear protein neun
    Angiotensin receptor type 2 is involved in heat acclimation (HA)-mediated enhanced neurogenesis in the subventricular zone (SVZ). Mice were subjected to traumatic brain injury (TBI) after treatment with saline or PD123319 (PD, 10 mg/kg/day) for 3 days, and killed 42 days post TBI. Evenly sliced 10- μ m sections were incubated with <t>anti-NeuN</t> for mature neurons and with <t>anti-BrdU</t> (anti-5-bromo-2-deoxyuridine) for newborn cells. ( A ) HA induced neurogenesis in the SVZ (i, j) and PD123319 (PD, 10 mg/kg/day) treatment blocked the observed neurogenesis (m–p). PD treatment (e–h) did not reduce neurogenesis in normothermic (NT) mice measured after saline treatment (a–d). Stereological quantification of the fields was used to count the number of BrdU-positive cells (BrdU + ). Results show the average number of BrdU + per field ( B ), and the average number of double-positive cells for NeuN and for BrdU (BrdU + /NeuN + ) per field ( C ). * P
    Neuron Specific Nuclear Protein Neun, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 221 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    N/A
    Affinity purified Chicken polyclonal NeuN antibody
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    Image Search Results


    Lack of colocalization of P2ry13 mRNA with other cell types of the DG (young adults). (A–E) Details of DG showing double labeling using FISH for P2ry13 (red) and immunocytochemistry for various cell markers (green); overlay (left), immunolabeling (right; all WT animals). (A) Glial fibrillary acidic protein (GFAP), (B) Sox2, (C) DCX (weak immunolabeling due to experimental conditions required for FISH) (D) NeuN. (E) Triple labeling for somatostatin (green, St), parvalbumin (white PV) and for P2ry13 (red). The GCL is outlined with dashed lines. Arrows, cell bodies labeled for P2ry13 mRNA; arrow head, P2ry13 -negative astrocyte. (F) Simplified schematic of principal cell types along the hippocampal neurogenesis pathway. Lineage markers used in this study and their cellular location are indicated by bars. gcl, granule cell layer; mol, molecular layer; sgz, subgranular zone. Scale bars, 25 μm.

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Disruption of the Microglial ADP Receptor P2Y13 Enhances Adult Hippocampal Neurogenesis

    doi: 10.3389/fncel.2018.00134

    Figure Lengend Snippet: Lack of colocalization of P2ry13 mRNA with other cell types of the DG (young adults). (A–E) Details of DG showing double labeling using FISH for P2ry13 (red) and immunocytochemistry for various cell markers (green); overlay (left), immunolabeling (right; all WT animals). (A) Glial fibrillary acidic protein (GFAP), (B) Sox2, (C) DCX (weak immunolabeling due to experimental conditions required for FISH) (D) NeuN. (E) Triple labeling for somatostatin (green, St), parvalbumin (white PV) and for P2ry13 (red). The GCL is outlined with dashed lines. Arrows, cell bodies labeled for P2ry13 mRNA; arrow head, P2ry13 -negative astrocyte. (F) Simplified schematic of principal cell types along the hippocampal neurogenesis pathway. Lineage markers used in this study and their cellular location are indicated by bars. gcl, granule cell layer; mol, molecular layer; sgz, subgranular zone. Scale bars, 25 μm.

    Article Snippet: Single- or two-color FISH combined with immunohistochemistry was performed as described (Ishii et al., ) with slight modifications: (1) hybridization step: slices were incubated in 12 μg/ml of proteinase K solution at 37°C for 16 min; (2) hybridization of P2ry13 and Cx3cr1 riboprobes labeled with digoxigenin and fluorescein, respectively, was performed at 65°C overnight in the hybridization solution (Parrilla et al., ); and (3) detection step: antibodies against digoxigenin-alkaline phosphatase (11093274910, Roche) and fluorescein horseradish peroxidase (NEF710, PerkinElmer) were incubated together with the following primary antibodies: anti-Iba1, anti-GFAP, anti-SRY (sex determining region Y)-box 2 (Sox2; Sc-17320, 1:200, Santa Cruz), anti-DCX, anti-NeuN (ABN78, 1:500, Millipore), anti-somatostatin (AB5494, 1:200, Millipore) and/or anti-parvalbumin (PV27, 1:200, Swant).

    Techniques: Labeling, Fluorescence In Situ Hybridization, Immunocytochemistry, Immunolabeling

    Impact of NIHL on neuronal differentiation in hippocampus. (A) representative images of EdU/NeuN double staining from the 4 subgroups. (B) Mean (±SEM) percentage of cells that were double labeled in the pool of cells labeled with EdU. ∗∗ p

    Journal: Frontiers in Systems Neuroscience

    Article Title: Hippocampal Mechanisms Underlying Impairment in Spatial Learning Long After Establishment of Noise-Induced Hearing Loss in CBA Mice

    doi: 10.3389/fnsys.2018.00035

    Figure Lengend Snippet: Impact of NIHL on neuronal differentiation in hippocampus. (A) representative images of EdU/NeuN double staining from the 4 subgroups. (B) Mean (±SEM) percentage of cells that were double labeled in the pool of cells labeled with EdU. ∗∗ p

    Article Snippet: After EdU staining, the slices were further stained with the first antibody against NeuN (1:500, Anti-NeuN, clone A60, mouse monoclonal, Millipore, MAB377, 4°C overnight) and then the second antibody [1:1000, Alexa Flour® 568 goat anti-mouse IgG1(y1), Invitrogen, , 37°C for 1 h].

    Techniques: Double Staining, Labeling

    Serotonergic dysfunction in Eif4e ki/ki mice. a Representative whole cell recordings of spontaneous excitatory post-synaptic currents (sEPSCs) from layer V pyramidal neurons of the medial prefrontal cortex (mPFC) of wild-type and Eif4e ki/ki mice. Traces represent consecutive recordings of synaptic currents before (baseline) and after 5-HT (20 μM) treatment from single pyramidal cells in each group. b Frequency of sEPSCs from pyramidal neurons of wild-type and Eif4e ki/ki mice before (−) and after 5-HT at 20 μM ( n = 7 cells from 5 Eif4e +/+ ; n = 8 cells from 7 Eif4e ki/ki), 50 μM ( n = 4 cells from 3 Eif4e +/+ ; n = 5 cells from 3 Eif4e ki/ki) or 100 μM ( n = 5 cells from 3 Eif4e +/+ ; n = 6 cells from 3 Eif4e ki/ki). c In vivo single-unit extracellular recordings of dorsal raphe neurons were performed in anesthetized Eif4e +/+ and ki/ki mice. d Firing rate of dorsal raphe 5-HT neurons, measured in Eif4e +/+ and ki/ki mice ( n = 56 single unit recordings from 8 Eif4e +/+ mice; n = 43 single unit recordings from 7 Eif4e ki/ki mice). e 5-HT neurons recorded per descent (track) in the dorsal raphe of wild-type and Eif4e ki/ki mice ( n = 8 Eif4e +/+ mice; n = 7 Eif4e ki/ki mice). f Firing rate of non-5-HT neurons in the DR ( n = 27 single unit recordings from 6 Eif4e +/+ mice; n = 16 single unit recordings from 5 Eif4e ki/ki mice). g Number of non-5-HT neurons per descent in the DR ( n = 6 Eif4e +/+ mice; n = 5 Eif4e ki/ki mice). h Number of NeuN + cells in the DR nuclei, which was subdivided into DR dorsal part (DRD), DR lateral part (DRL), posterodorsal raphe nucleus (PRN), and DR ventral part (DRV). Data are means of 4 slides per animal ( n = 4 Eif4e +/+ mice; n = 5 Eif4e ki/ki mice). i Number of tryptophan hydroxylase 2 (TPH2) positive cells in the DRN. j Representative images of the DR IHC; blue: nuclear DAPI staining; red: TPH2; green: NeuN. Scale bar is 100 μM. * p

    Journal: Nature Communications

    Article Title: Translational control of depression-like behavior via phosphorylation of eukaryotic translation initiation factor 4E

    doi: 10.1038/s41467-018-04883-5

    Figure Lengend Snippet: Serotonergic dysfunction in Eif4e ki/ki mice. a Representative whole cell recordings of spontaneous excitatory post-synaptic currents (sEPSCs) from layer V pyramidal neurons of the medial prefrontal cortex (mPFC) of wild-type and Eif4e ki/ki mice. Traces represent consecutive recordings of synaptic currents before (baseline) and after 5-HT (20 μM) treatment from single pyramidal cells in each group. b Frequency of sEPSCs from pyramidal neurons of wild-type and Eif4e ki/ki mice before (−) and after 5-HT at 20 μM ( n = 7 cells from 5 Eif4e +/+ ; n = 8 cells from 7 Eif4e ki/ki), 50 μM ( n = 4 cells from 3 Eif4e +/+ ; n = 5 cells from 3 Eif4e ki/ki) or 100 μM ( n = 5 cells from 3 Eif4e +/+ ; n = 6 cells from 3 Eif4e ki/ki). c In vivo single-unit extracellular recordings of dorsal raphe neurons were performed in anesthetized Eif4e +/+ and ki/ki mice. d Firing rate of dorsal raphe 5-HT neurons, measured in Eif4e +/+ and ki/ki mice ( n = 56 single unit recordings from 8 Eif4e +/+ mice; n = 43 single unit recordings from 7 Eif4e ki/ki mice). e 5-HT neurons recorded per descent (track) in the dorsal raphe of wild-type and Eif4e ki/ki mice ( n = 8 Eif4e +/+ mice; n = 7 Eif4e ki/ki mice). f Firing rate of non-5-HT neurons in the DR ( n = 27 single unit recordings from 6 Eif4e +/+ mice; n = 16 single unit recordings from 5 Eif4e ki/ki mice). g Number of non-5-HT neurons per descent in the DR ( n = 6 Eif4e +/+ mice; n = 5 Eif4e ki/ki mice). h Number of NeuN + cells in the DR nuclei, which was subdivided into DR dorsal part (DRD), DR lateral part (DRL), posterodorsal raphe nucleus (PRN), and DR ventral part (DRV). Data are means of 4 slides per animal ( n = 4 Eif4e +/+ mice; n = 5 Eif4e ki/ki mice). i Number of tryptophan hydroxylase 2 (TPH2) positive cells in the DRN. j Representative images of the DR IHC; blue: nuclear DAPI staining; red: TPH2; green: NeuN. Scale bar is 100 μM. * p

    Article Snippet: Antibodies were: polyclonal rabbit anti-TPH2 (Novus Biologicals, 1:1000) and monoclonal mouse anti-NeuN (Millipore, 1:150).

    Techniques: Mouse Assay, In Vivo, Immunohistochemistry, Staining

    Exercise enhances proliferation of neurons in the hippocampal dentate gyrus. (A) Immunofluorescence staining for BrdU (red) and NeuN (green) in rats 21 days after MCAO. Scale bar=50 µm. (B) Quantification of BrdU + /NeuN + cells 7, 14 and 21 days after MCAO. Data are presented as the means ± standard deviation. The arrows in the images are indicating BrdU + /NeuN + cells. *P

    Journal: Molecular Medicine Reports

    Article Title: Physical exercise promotes proliferation and differentiation of endogenous neural stem cells via ERK in rats with cerebral infarction

    doi: 10.3892/mmr.2018.9147

    Figure Lengend Snippet: Exercise enhances proliferation of neurons in the hippocampal dentate gyrus. (A) Immunofluorescence staining for BrdU (red) and NeuN (green) in rats 21 days after MCAO. Scale bar=50 µm. (B) Quantification of BrdU + /NeuN + cells 7, 14 and 21 days after MCAO. Data are presented as the means ± standard deviation. The arrows in the images are indicating BrdU + /NeuN + cells. *P

    Article Snippet: The sections were incubated at 4°C overnight with the following primary antibodies: Mouse anti-BrdU (1:100; cat. no. B2531; Sigma-Aldrich; Merck KGaA), rabbit anti-NeuN (1:200; cat. no. ab177487) and rabbit anti-GFAP (1:500; cat. no. ab7260; both Abcam, Cambridge, MA, USA).

    Techniques: Immunofluorescence, Staining, Standard Deviation

    Analysis of transduced neurons after i Mecp2 gene transfer in Mecp2 -/y brains. ( a ) Characterization of i Mecp2 -transduced cells in KO cortex (1×10 9 , 1×10 10 , 1×10 11 ,1×10 12 vg/mouse) using colocalization of V5 + cells with a neuronal marker (NeuN). ( b ) Quantification of V5 + cells on total DAPI + cells (orange bar) and V5 + /NeuN + cells on total DAPI + cells (green bar). Dashed line indicates the number of NeuN + cells on total DAPI + cells. ( c–d ) Colocalization and quantification of transduced (V5+) GABAerigic interneurons (marked with GABA, GABA + ) in i Mecp2 -transduced KO cortexes of animals treated with a 1×10 11 vg/mouse dose. Nuclei were stained with DAPI. All images were captured using confocal microscope. Scale bar: 50 μm. n = 3 animals per group.

    Journal: eLife

    Article Title: Whole brain delivery of an instability-prone Mecp2 transgene improves behavioral and molecular pathological defects in mouse models of Rett syndrome

    doi: 10.7554/eLife.52629

    Figure Lengend Snippet: Analysis of transduced neurons after i Mecp2 gene transfer in Mecp2 -/y brains. ( a ) Characterization of i Mecp2 -transduced cells in KO cortex (1×10 9 , 1×10 10 , 1×10 11 ,1×10 12 vg/mouse) using colocalization of V5 + cells with a neuronal marker (NeuN). ( b ) Quantification of V5 + cells on total DAPI + cells (orange bar) and V5 + /NeuN + cells on total DAPI + cells (green bar). Dashed line indicates the number of NeuN + cells on total DAPI + cells. ( c–d ) Colocalization and quantification of transduced (V5+) GABAerigic interneurons (marked with GABA, GABA + ) in i Mecp2 -transduced KO cortexes of animals treated with a 1×10 11 vg/mouse dose. Nuclei were stained with DAPI. All images were captured using confocal microscope. Scale bar: 50 μm. n = 3 animals per group.

    Article Snippet: Cell and tissue where stained with the following primary antibody: rabbit anti-MeCP2 (1:500; Cell Signaling Technology, RRID: AB_2143849 ), mouse anti-V5 (1:500; ThermoFisher Scientific, RRID: AB_2556564 ), mouse anti-NeuN (1:300; Merck Millipore, RRID: AB_2298772 ), rabbit anti-Sox9 (1:500; Sigma-Aldrich, RRID: AB_2239761 ), rabbit anti-GABA (1:500; Sigma-Aldrich, RRID: AB_477652 ) anti-chicken_MAP2 (1:500, Abcam, RRID: AB_2138147 ), anti-OCT4 (1:50, Abcam, RRID: AB_444714 ).

    Techniques: Marker, Staining, Microscopy

    Characterization of i Mecp2 transgene expression in brain and liver of Mecp2 +/- animals. ( a ) Vector biodistribution (upper panel) and transgene expression (lower panel) in mice cortex and liver of wild-type female mice (WT female, n = 3), Het mice untreated (Het, n = 3) and treated with 1×10 11 (n = 3) of i Mecp2 . Data were normalized over Mecp2 gene levels and expression of wild-type mice (WT male, n = 3), respectively. ( b ) Characterization of i Mecp2 -transduced cells in Het cortex (1×10 11 vg/mouse) using colocalization of V5 + cells with neurons (NeuN+) and astrocytes (Sox9). ( c ) Quantification of V5+ cells on total DAPI+ cells (orange bar), V5+ NeuN+ cells on total DAPI+ cells (green bar) and V5+ Sox9+ cells on total DAPI+ cells (blue bar). Dashed lines indicate the number of NeuN+ cells or Sox9+ cells on total DAPI + cells. Nuclei were stained with DAPI. All images were captured using confocal microscope. Scale bar: 50 μm. N = 3 animals per group.

    Journal: eLife

    Article Title: Whole brain delivery of an instability-prone Mecp2 transgene improves behavioral and molecular pathological defects in mouse models of Rett syndrome

    doi: 10.7554/eLife.52629

    Figure Lengend Snippet: Characterization of i Mecp2 transgene expression in brain and liver of Mecp2 +/- animals. ( a ) Vector biodistribution (upper panel) and transgene expression (lower panel) in mice cortex and liver of wild-type female mice (WT female, n = 3), Het mice untreated (Het, n = 3) and treated with 1×10 11 (n = 3) of i Mecp2 . Data were normalized over Mecp2 gene levels and expression of wild-type mice (WT male, n = 3), respectively. ( b ) Characterization of i Mecp2 -transduced cells in Het cortex (1×10 11 vg/mouse) using colocalization of V5 + cells with neurons (NeuN+) and astrocytes (Sox9). ( c ) Quantification of V5+ cells on total DAPI+ cells (orange bar), V5+ NeuN+ cells on total DAPI+ cells (green bar) and V5+ Sox9+ cells on total DAPI+ cells (blue bar). Dashed lines indicate the number of NeuN+ cells or Sox9+ cells on total DAPI + cells. Nuclei were stained with DAPI. All images were captured using confocal microscope. Scale bar: 50 μm. N = 3 animals per group.

    Article Snippet: Cell and tissue where stained with the following primary antibody: rabbit anti-MeCP2 (1:500; Cell Signaling Technology, RRID: AB_2143849 ), mouse anti-V5 (1:500; ThermoFisher Scientific, RRID: AB_2556564 ), mouse anti-NeuN (1:300; Merck Millipore, RRID: AB_2298772 ), rabbit anti-Sox9 (1:500; Sigma-Aldrich, RRID: AB_2239761 ), rabbit anti-GABA (1:500; Sigma-Aldrich, RRID: AB_477652 ) anti-chicken_MAP2 (1:500, Abcam, RRID: AB_2138147 ), anti-OCT4 (1:50, Abcam, RRID: AB_444714 ).

    Techniques: Expressing, Plasmid Preparation, Mouse Assay, Staining, Microscopy

    Angiotensin receptor type 2 is involved in heat acclimation (HA)-mediated enhanced neurogenesis in the subventricular zone (SVZ). Mice were subjected to traumatic brain injury (TBI) after treatment with saline or PD123319 (PD, 10 mg/kg/day) for 3 days, and killed 42 days post TBI. Evenly sliced 10- μ m sections were incubated with anti-NeuN for mature neurons and with anti-BrdU (anti-5-bromo-2-deoxyuridine) for newborn cells. ( A ) HA induced neurogenesis in the SVZ (i, j) and PD123319 (PD, 10 mg/kg/day) treatment blocked the observed neurogenesis (m–p). PD treatment (e–h) did not reduce neurogenesis in normothermic (NT) mice measured after saline treatment (a–d). Stereological quantification of the fields was used to count the number of BrdU-positive cells (BrdU + ). Results show the average number of BrdU + per field ( B ), and the average number of double-positive cells for NeuN and for BrdU (BrdU + /NeuN + ) per field ( C ). * P

    Journal: Journal of Cerebral Blood Flow & Metabolism

    Article Title: Neuroprotection after traumatic brain injury in heat-acclimated mice involves induced neurogenesis and activation of angiotensin receptor type 2 signaling

    doi: 10.1038/jcbfm.2014.93

    Figure Lengend Snippet: Angiotensin receptor type 2 is involved in heat acclimation (HA)-mediated enhanced neurogenesis in the subventricular zone (SVZ). Mice were subjected to traumatic brain injury (TBI) after treatment with saline or PD123319 (PD, 10 mg/kg/day) for 3 days, and killed 42 days post TBI. Evenly sliced 10- μ m sections were incubated with anti-NeuN for mature neurons and with anti-BrdU (anti-5-bromo-2-deoxyuridine) for newborn cells. ( A ) HA induced neurogenesis in the SVZ (i, j) and PD123319 (PD, 10 mg/kg/day) treatment blocked the observed neurogenesis (m–p). PD treatment (e–h) did not reduce neurogenesis in normothermic (NT) mice measured after saline treatment (a–d). Stereological quantification of the fields was used to count the number of BrdU-positive cells (BrdU + ). Results show the average number of BrdU + per field ( B ), and the average number of double-positive cells for NeuN and for BrdU (BrdU + /NeuN + ) per field ( C ). * P

    Article Snippet: Brain slices were double stained for immunohistochemical evaluation using fate-specific antibodies anti-BrdU (2 μ g/mL; Calbiochem, Darmstadt, Germany) and anti-NeuN (1:750; Merck Millipore, Darmstadt, Germany).

    Techniques: Mouse Assay, Incubation

    Angiotensin receptor type 2 is involved in heat acclimation (HA)-mediated enhanced neurogenesis in the injury region (inj). Mice were subjected to traumatic brain injury (TBI) and treated with saline or PD123319 (PD, 10 mg/kg/day) for 3 days, and killed 42 days post TBI. Evenly sliced 10- μ m sections were incubated with anti-NeuN for mature neurons and with anti-BrdU (anti-5-bromo-2-deoxyuridine) for newborn cells. ( A ) HA induced neurogenesis in the injury region (i, j) and PD123319 (PD, 10 mg/kg/day) treatment blocked the observed neurogenesis (m–p). PD treatment (e–h) did not reduce neurogenesis in normothermic (NT) mice (a–d). Stereological quantification of the fields was used to count the number of BrdU-positive cells (BrdU + ). Results show the average number of BrdU + per field ( B ), and the average number of double-positive cells for NeuN and for BrdU (BrdU + /NeuN + ) per field ( C ). * P

    Journal: Journal of Cerebral Blood Flow & Metabolism

    Article Title: Neuroprotection after traumatic brain injury in heat-acclimated mice involves induced neurogenesis and activation of angiotensin receptor type 2 signaling

    doi: 10.1038/jcbfm.2014.93

    Figure Lengend Snippet: Angiotensin receptor type 2 is involved in heat acclimation (HA)-mediated enhanced neurogenesis in the injury region (inj). Mice were subjected to traumatic brain injury (TBI) and treated with saline or PD123319 (PD, 10 mg/kg/day) for 3 days, and killed 42 days post TBI. Evenly sliced 10- μ m sections were incubated with anti-NeuN for mature neurons and with anti-BrdU (anti-5-bromo-2-deoxyuridine) for newborn cells. ( A ) HA induced neurogenesis in the injury region (i, j) and PD123319 (PD, 10 mg/kg/day) treatment blocked the observed neurogenesis (m–p). PD treatment (e–h) did not reduce neurogenesis in normothermic (NT) mice (a–d). Stereological quantification of the fields was used to count the number of BrdU-positive cells (BrdU + ). Results show the average number of BrdU + per field ( B ), and the average number of double-positive cells for NeuN and for BrdU (BrdU + /NeuN + ) per field ( C ). * P

    Article Snippet: Brain slices were double stained for immunohistochemical evaluation using fate-specific antibodies anti-BrdU (2 μ g/mL; Calbiochem, Darmstadt, Germany) and anti-NeuN (1:750; Merck Millipore, Darmstadt, Germany).

    Techniques: Mouse Assay, Incubation

    Angiotensin receptor type 2 is involved in heat acclimation (HA)-mediated enhanced neurogenesis in the dentate gyrus. Mice were subjected to traumatic brain injury (TBI) and treated with saline or PD123319 (PD, 10 mg/kg/day) for 3 days, and killed 42 days post TBI. Evenly sliced 10- μ m sections were incubated with anti-NeuN for mature neurons and with anti-BrdU (anti-5-bromo-2-deoxyuridine) for newborn cells. ( A ) HA induced neurogenesis in the dentate gyrus subgranular zone (i, j) and PD123319 (PD, 10 mg/kg/day) treatment blocked the observed neurogenesis (m–p). PD treatment (e–h) did not reduce neurogenesis in normothermic (NT) mice (a–d). Stereological quantification of the fields was used to count the number of BrdU-positive cells (BrdU + ). Results show the average number of BrdU + per field ( B ), and the average number of double-positive cells for NeuN and for BrdU (BrdU + /NeuN + ) per field ( C ). * P

    Journal: Journal of Cerebral Blood Flow & Metabolism

    Article Title: Neuroprotection after traumatic brain injury in heat-acclimated mice involves induced neurogenesis and activation of angiotensin receptor type 2 signaling

    doi: 10.1038/jcbfm.2014.93

    Figure Lengend Snippet: Angiotensin receptor type 2 is involved in heat acclimation (HA)-mediated enhanced neurogenesis in the dentate gyrus. Mice were subjected to traumatic brain injury (TBI) and treated with saline or PD123319 (PD, 10 mg/kg/day) for 3 days, and killed 42 days post TBI. Evenly sliced 10- μ m sections were incubated with anti-NeuN for mature neurons and with anti-BrdU (anti-5-bromo-2-deoxyuridine) for newborn cells. ( A ) HA induced neurogenesis in the dentate gyrus subgranular zone (i, j) and PD123319 (PD, 10 mg/kg/day) treatment blocked the observed neurogenesis (m–p). PD treatment (e–h) did not reduce neurogenesis in normothermic (NT) mice (a–d). Stereological quantification of the fields was used to count the number of BrdU-positive cells (BrdU + ). Results show the average number of BrdU + per field ( B ), and the average number of double-positive cells for NeuN and for BrdU (BrdU + /NeuN + ) per field ( C ). * P

    Article Snippet: Brain slices were double stained for immunohistochemical evaluation using fate-specific antibodies anti-BrdU (2 μ g/mL; Calbiochem, Darmstadt, Germany) and anti-NeuN (1:750; Merck Millipore, Darmstadt, Germany).

    Techniques: Mouse Assay, Incubation