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  • 99
    Integrated DNA Technologies dna oligonucleotides
    Working principle of basic <t>NESA.</t> Single-stranded target <t>DNA</t> contains the recognition sequence of a nicking enzyme. A molecular beacon is designed with its loop sequence complementary to the target. When one target molecule hybridizes with one beacon molecule, a full recognition site forms for the nicking enzyme to cleave the beacon strand. The nicking enzyme binds to the hybrid, and makes a nick in the beacon strand. After nicking, the complex dissociates, finishing one reaction cycle. The net result of one reaction cycle is a cleaved molecular beacon. The target molecule and nicking enzyme can be re-used for next cycle of cleavage. This way, each target can go through many cycles, resulting in cleavage of many beacon molecules. In each cycle, one target causes one beacon molecule to open and fluoresce, contributing one beacon signal. After N ( N is an integer) cycles, one target gives rise to N beacons signals, achieving a linear signal amplification.
    Dna Oligonucleotides, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 99/100, based on 2965 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna oligonucleotides/product/Integrated DNA Technologies
    Average 99 stars, based on 2965 article reviews
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    93
    New England Biolabs nt bsmai
    Working principle of basic <t>NESA.</t> Single-stranded target <t>DNA</t> contains the recognition sequence of a nicking enzyme. A molecular beacon is designed with its loop sequence complementary to the target. When one target molecule hybridizes with one beacon molecule, a full recognition site forms for the nicking enzyme to cleave the beacon strand. The nicking enzyme binds to the hybrid, and makes a nick in the beacon strand. After nicking, the complex dissociates, finishing one reaction cycle. The net result of one reaction cycle is a cleaved molecular beacon. The target molecule and nicking enzyme can be re-used for next cycle of cleavage. This way, each target can go through many cycles, resulting in cleavage of many beacon molecules. In each cycle, one target causes one beacon molecule to open and fluoresce, contributing one beacon signal. After N ( N is an integer) cycles, one target gives rise to N beacons signals, achieving a linear signal amplification.
    Nt Bsmai, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nt bsmai/product/New England Biolabs
    Average 93 stars, based on 21 article reviews
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    nt bsmai - by Bioz Stars, 2020-08
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    93
    Medtronic calambur narasimhan india medtronic pvt
    Working principle of basic <t>NESA.</t> Single-stranded target <t>DNA</t> contains the recognition sequence of a nicking enzyme. A molecular beacon is designed with its loop sequence complementary to the target. When one target molecule hybridizes with one beacon molecule, a full recognition site forms for the nicking enzyme to cleave the beacon strand. The nicking enzyme binds to the hybrid, and makes a nick in the beacon strand. After nicking, the complex dissociates, finishing one reaction cycle. The net result of one reaction cycle is a cleaved molecular beacon. The target molecule and nicking enzyme can be re-used for next cycle of cleavage. This way, each target can go through many cycles, resulting in cleavage of many beacon molecules. In each cycle, one target causes one beacon molecule to open and fluoresce, contributing one beacon signal. After N ( N is an integer) cycles, one target gives rise to N beacons signals, achieving a linear signal amplification.
    Calambur Narasimhan India Medtronic Pvt, supplied by Medtronic, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/calambur narasimhan india medtronic pvt/product/Medtronic
    Average 93 stars, based on 1 article reviews
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    93
    Medtronic ajay naik india medtronic pvt
    Working principle of basic <t>NESA.</t> Single-stranded target <t>DNA</t> contains the recognition sequence of a nicking enzyme. A molecular beacon is designed with its loop sequence complementary to the target. When one target molecule hybridizes with one beacon molecule, a full recognition site forms for the nicking enzyme to cleave the beacon strand. The nicking enzyme binds to the hybrid, and makes a nick in the beacon strand. After nicking, the complex dissociates, finishing one reaction cycle. The net result of one reaction cycle is a cleaved molecular beacon. The target molecule and nicking enzyme can be re-used for next cycle of cleavage. This way, each target can go through many cycles, resulting in cleavage of many beacon molecules. In each cycle, one target causes one beacon molecule to open and fluoresce, contributing one beacon signal. After N ( N is an integer) cycles, one target gives rise to N beacons signals, achieving a linear signal amplification.
    Ajay Naik India Medtronic Pvt, supplied by Medtronic, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ajay naik india medtronic pvt/product/Medtronic
    Average 93 stars, based on 1 article reviews
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    ajay naik india medtronic pvt - by Bioz Stars, 2020-08
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    85
    Elekta cifre contract
    Working principle of basic <t>NESA.</t> Single-stranded target <t>DNA</t> contains the recognition sequence of a nicking enzyme. A molecular beacon is designed with its loop sequence complementary to the target. When one target molecule hybridizes with one beacon molecule, a full recognition site forms for the nicking enzyme to cleave the beacon strand. The nicking enzyme binds to the hybrid, and makes a nick in the beacon strand. After nicking, the complex dissociates, finishing one reaction cycle. The net result of one reaction cycle is a cleaved molecular beacon. The target molecule and nicking enzyme can be re-used for next cycle of cleavage. This way, each target can go through many cycles, resulting in cleavage of many beacon molecules. In each cycle, one target causes one beacon molecule to open and fluoresce, contributing one beacon signal. After N ( N is an integer) cycles, one target gives rise to N beacons signals, achieving a linear signal amplification.
    Cifre Contract, supplied by Elekta, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    HORIBA spectrofluorometer
    Working principle of basic <t>NESA.</t> Single-stranded target <t>DNA</t> contains the recognition sequence of a nicking enzyme. A molecular beacon is designed with its loop sequence complementary to the target. When one target molecule hybridizes with one beacon molecule, a full recognition site forms for the nicking enzyme to cleave the beacon strand. The nicking enzyme binds to the hybrid, and makes a nick in the beacon strand. After nicking, the complex dissociates, finishing one reaction cycle. The net result of one reaction cycle is a cleaved molecular beacon. The target molecule and nicking enzyme can be re-used for next cycle of cleavage. This way, each target can go through many cycles, resulting in cleavage of many beacon molecules. In each cycle, one target causes one beacon molecule to open and fluoresce, contributing one beacon signal. After N ( N is an integer) cycles, one target gives rise to N beacons signals, achieving a linear signal amplification.
    Spectrofluorometer, supplied by HORIBA, used in various techniques. Bioz Stars score: 93/100, based on 455 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Stratagene real time pcr machine
    Working principle of basic <t>NESA.</t> Single-stranded target <t>DNA</t> contains the recognition sequence of a nicking enzyme. A molecular beacon is designed with its loop sequence complementary to the target. When one target molecule hybridizes with one beacon molecule, a full recognition site forms for the nicking enzyme to cleave the beacon strand. The nicking enzyme binds to the hybrid, and makes a nick in the beacon strand. After nicking, the complex dissociates, finishing one reaction cycle. The net result of one reaction cycle is a cleaved molecular beacon. The target molecule and nicking enzyme can be re-used for next cycle of cleavage. This way, each target can go through many cycles, resulting in cleavage of many beacon molecules. In each cycle, one target causes one beacon molecule to open and fluoresce, contributing one beacon signal. After N ( N is an integer) cycles, one target gives rise to N beacons signals, achieving a linear signal amplification.
    Real Time Pcr Machine, supplied by Stratagene, used in various techniques. Bioz Stars score: 93/100, based on 241 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher promastigotes
    Working principle of basic <t>NESA.</t> Single-stranded target <t>DNA</t> contains the recognition sequence of a nicking enzyme. A molecular beacon is designed with its loop sequence complementary to the target. When one target molecule hybridizes with one beacon molecule, a full recognition site forms for the nicking enzyme to cleave the beacon strand. The nicking enzyme binds to the hybrid, and makes a nick in the beacon strand. After nicking, the complex dissociates, finishing one reaction cycle. The net result of one reaction cycle is a cleaved molecular beacon. The target molecule and nicking enzyme can be re-used for next cycle of cleavage. This way, each target can go through many cycles, resulting in cleavage of many beacon molecules. In each cycle, one target causes one beacon molecule to open and fluoresce, contributing one beacon signal. After N ( N is an integer) cycles, one target gives rise to N beacons signals, achieving a linear signal amplification.
    Promastigotes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1498 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    SAS institute statistical software version 9 1
    Working principle of basic <t>NESA.</t> Single-stranded target <t>DNA</t> contains the recognition sequence of a nicking enzyme. A molecular beacon is designed with its loop sequence complementary to the target. When one target molecule hybridizes with one beacon molecule, a full recognition site forms for the nicking enzyme to cleave the beacon strand. The nicking enzyme binds to the hybrid, and makes a nick in the beacon strand. After nicking, the complex dissociates, finishing one reaction cycle. The net result of one reaction cycle is a cleaved molecular beacon. The target molecule and nicking enzyme can be re-used for next cycle of cleavage. This way, each target can go through many cycles, resulting in cleavage of many beacon molecules. In each cycle, one target causes one beacon molecule to open and fluoresce, contributing one beacon signal. After N ( N is an integer) cycles, one target gives rise to N beacons signals, achieving a linear signal amplification.
    Statistical Software Version 9 1, supplied by SAS institute, used in various techniques. Bioz Stars score: 91/100, based on 454 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    statistical software version 9 1 - by Bioz Stars, 2020-08
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    94
    Roche light cycler 480
    Working principle of basic <t>NESA.</t> Single-stranded target <t>DNA</t> contains the recognition sequence of a nicking enzyme. A molecular beacon is designed with its loop sequence complementary to the target. When one target molecule hybridizes with one beacon molecule, a full recognition site forms for the nicking enzyme to cleave the beacon strand. The nicking enzyme binds to the hybrid, and makes a nick in the beacon strand. After nicking, the complex dissociates, finishing one reaction cycle. The net result of one reaction cycle is a cleaved molecular beacon. The target molecule and nicking enzyme can be re-used for next cycle of cleavage. This way, each target can go through many cycles, resulting in cleavage of many beacon molecules. In each cycle, one target causes one beacon molecule to open and fluoresce, contributing one beacon signal. After N ( N is an integer) cycles, one target gives rise to N beacons signals, achieving a linear signal amplification.
    Light Cycler 480, supplied by Roche, used in various techniques. Bioz Stars score: 94/100, based on 12833 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Integrated DNA Technologies bsub 6
    Limit of detection using bacterial cells. A log phase culture of B. subtilis was serially diluted and 1 µl of each dilution was used to determine colony forming units by growth overnight on LB plates. Another 1 µl aliquot from each of these dilutions was amplified directly with multiple displacement amplification, without sample clean up, then underwent NESA analysis with both Bsub 3 and <t>Bsub</t> 6 probes.
    Bsub 6, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bsub 6/product/Integrated DNA Technologies
    Average 85 stars, based on 5 article reviews
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    Image Search Results


    Working principle of basic NESA. Single-stranded target DNA contains the recognition sequence of a nicking enzyme. A molecular beacon is designed with its loop sequence complementary to the target. When one target molecule hybridizes with one beacon molecule, a full recognition site forms for the nicking enzyme to cleave the beacon strand. The nicking enzyme binds to the hybrid, and makes a nick in the beacon strand. After nicking, the complex dissociates, finishing one reaction cycle. The net result of one reaction cycle is a cleaved molecular beacon. The target molecule and nicking enzyme can be re-used for next cycle of cleavage. This way, each target can go through many cycles, resulting in cleavage of many beacon molecules. In each cycle, one target causes one beacon molecule to open and fluoresce, contributing one beacon signal. After N ( N is an integer) cycles, one target gives rise to N beacons signals, achieving a linear signal amplification.

    Journal: Nucleic Acids Research

    Article Title: Enzymatic signal amplification of molecular beacons for sensitive DNA detection

    doi: 10.1093/nar/gkn033

    Figure Lengend Snippet: Working principle of basic NESA. Single-stranded target DNA contains the recognition sequence of a nicking enzyme. A molecular beacon is designed with its loop sequence complementary to the target. When one target molecule hybridizes with one beacon molecule, a full recognition site forms for the nicking enzyme to cleave the beacon strand. The nicking enzyme binds to the hybrid, and makes a nick in the beacon strand. After nicking, the complex dissociates, finishing one reaction cycle. The net result of one reaction cycle is a cleaved molecular beacon. The target molecule and nicking enzyme can be re-used for next cycle of cleavage. This way, each target can go through many cycles, resulting in cleavage of many beacon molecules. In each cycle, one target causes one beacon molecule to open and fluoresce, contributing one beacon signal. After N ( N is an integer) cycles, one target gives rise to N beacons signals, achieving a linear signal amplification.

    Article Snippet: Molecular beacons and DNA oligonucleotides for NESA, hybridization and RCA assays were synthesized by Integrated DNA Technologies; all other oligonucleotides were from MWG Biotech.

    Techniques: Sequencing, Amplification

    Working principle of extended NESA. Basic NESA is integrated with rolling circle amplification (RCA), in order to recognize target DNA of any sequence of interest. The central element in RCA is the padlock probe, which contains, at its two ends, a target recognition sequence, and, in the middle, a sequence (green color) identical to that of the major portion (loop plus one arm) of a molecular beacon. The molecular beacon contains a nicking enzyme recognition sequence. Extended NESA includes three sequential steps: ligation, polymerization and nicking. At ligation step, a padlock probe hybridizes at two ends to target DNA and is circularized by DNA ligase. At polymerization step, a primer binds to the circularized padlock probe, and is extended by DNA polymerase, producing a long single-stranded DNA composed of tandem copies of the complementary sequence of the padlock probe, with each copy containing a complementary sequence (red color) for the molecular beacon. At nicking step, each red color sequence, like the target sequence in basic NESA, acts as a mobile catalytic site, and leads to the nicking of many molecular beacons in the presence of nicking enzymes. To be consistent with the terms used in the basic nicking assay, we designated the target DNA as the primary target, and the red color sequence in the RCA product the secondary target. In extended NESA, the primary target does not need to contain nicking enzyme recognition sequence. Extended NESA has two levels of signal amplification: each primary target induces many secondary targets through RCA, and each secondary target brings about cleavage of many beacon molecules.

    Journal: Nucleic Acids Research

    Article Title: Enzymatic signal amplification of molecular beacons for sensitive DNA detection

    doi: 10.1093/nar/gkn033

    Figure Lengend Snippet: Working principle of extended NESA. Basic NESA is integrated with rolling circle amplification (RCA), in order to recognize target DNA of any sequence of interest. The central element in RCA is the padlock probe, which contains, at its two ends, a target recognition sequence, and, in the middle, a sequence (green color) identical to that of the major portion (loop plus one arm) of a molecular beacon. The molecular beacon contains a nicking enzyme recognition sequence. Extended NESA includes three sequential steps: ligation, polymerization and nicking. At ligation step, a padlock probe hybridizes at two ends to target DNA and is circularized by DNA ligase. At polymerization step, a primer binds to the circularized padlock probe, and is extended by DNA polymerase, producing a long single-stranded DNA composed of tandem copies of the complementary sequence of the padlock probe, with each copy containing a complementary sequence (red color) for the molecular beacon. At nicking step, each red color sequence, like the target sequence in basic NESA, acts as a mobile catalytic site, and leads to the nicking of many molecular beacons in the presence of nicking enzymes. To be consistent with the terms used in the basic nicking assay, we designated the target DNA as the primary target, and the red color sequence in the RCA product the secondary target. In extended NESA, the primary target does not need to contain nicking enzyme recognition sequence. Extended NESA has two levels of signal amplification: each primary target induces many secondary targets through RCA, and each secondary target brings about cleavage of many beacon molecules.

    Article Snippet: Molecular beacons and DNA oligonucleotides for NESA, hybridization and RCA assays were synthesized by Integrated DNA Technologies; all other oligonucleotides were from MWG Biotech.

    Techniques: Amplification, Sequencing, Ligation

    Limit of detection using bacterial cells. A log phase culture of B. subtilis was serially diluted and 1 µl of each dilution was used to determine colony forming units by growth overnight on LB plates. Another 1 µl aliquot from each of these dilutions was amplified directly with multiple displacement amplification, without sample clean up, then underwent NESA analysis with both Bsub 3 and Bsub 6 probes.

    Journal: Nucleic Acids Research

    Article Title: Sequence specific detection of DNA using nicking endonuclease signal amplification (NESA)

    doi: 10.1093/nar/gkm654

    Figure Lengend Snippet: Limit of detection using bacterial cells. A log phase culture of B. subtilis was serially diluted and 1 µl of each dilution was used to determine colony forming units by growth overnight on LB plates. Another 1 µl aliquot from each of these dilutions was amplified directly with multiple displacement amplification, without sample clean up, then underwent NESA analysis with both Bsub 3 and Bsub 6 probes.

    Article Snippet: We used two 21-mer B. subtilis probes, Bsub 3 and Bsub 6, that have a standard T m of 50.6 and 56.3°C, respectively (Integrated DNA Technologies Inc).

    Techniques: Amplification, Multiple Displacement Amplification

    Multiplex assays. Standard NESA reactions were set up using either oligonucleotide complements ( A–E ) or 500 ng B. subtilis MDA DNA. Peaks labeled 3 and 6 are derived from FAM-labeled probes Bsub 3 and Bsub 6, respectively (blue lines). Peaks labeled 3T and 6T are derived from FAM-labeled Bsub 3 and Bsub 6 probes with two additional nucleotides (T 2 ) at the 5′ end. Peaks labeled 3H and 6H are HEX-labeled Bsub 3 and Bsub 6 probes, respectively (green lines). The positions of the 5 and 17 base hex-labeled standards are shown. The cleaved probes migrate at positions that cannot be determined solely by their length. The apparent sizes of the cleaved probes are: FAM-Bsub 3, 13.4 bases; FAM-Bsub 6, 13.6 bases; FAM-Bsub 3T2, 14.6 bases; FAM-Bsub 6T2, 14.4 bases; HEX-Bsub 3 bases, 14.5; HEX-BSub 6, 15.1 bases.

    Journal: Nucleic Acids Research

    Article Title: Sequence specific detection of DNA using nicking endonuclease signal amplification (NESA)

    doi: 10.1093/nar/gkm654

    Figure Lengend Snippet: Multiplex assays. Standard NESA reactions were set up using either oligonucleotide complements ( A–E ) or 500 ng B. subtilis MDA DNA. Peaks labeled 3 and 6 are derived from FAM-labeled probes Bsub 3 and Bsub 6, respectively (blue lines). Peaks labeled 3T and 6T are derived from FAM-labeled Bsub 3 and Bsub 6 probes with two additional nucleotides (T 2 ) at the 5′ end. Peaks labeled 3H and 6H are HEX-labeled Bsub 3 and Bsub 6 probes, respectively (green lines). The positions of the 5 and 17 base hex-labeled standards are shown. The cleaved probes migrate at positions that cannot be determined solely by their length. The apparent sizes of the cleaved probes are: FAM-Bsub 3, 13.4 bases; FAM-Bsub 6, 13.6 bases; FAM-Bsub 3T2, 14.6 bases; FAM-Bsub 6T2, 14.4 bases; HEX-Bsub 3 bases, 14.5; HEX-BSub 6, 15.1 bases.

    Article Snippet: We used two 21-mer B. subtilis probes, Bsub 3 and Bsub 6, that have a standard T m of 50.6 and 56.3°C, respectively (Integrated DNA Technologies Inc).

    Techniques: Multiplex Assay, Multiple Displacement Amplification, Labeling, Derivative Assay

    Probe specificity. Standard NESA reactions were performed using 0.2 fmol of genomic DNA from various Bacillus species. No cut probe was detected in the absence of added DNA or using the Bsub 6 probe with B. anthracis, B. cereus or B thuringiensis genomic DNA. *, significantly different ( t -test) from the no added DNA control ( P

    Journal: Nucleic Acids Research

    Article Title: Sequence specific detection of DNA using nicking endonuclease signal amplification (NESA)

    doi: 10.1093/nar/gkm654

    Figure Lengend Snippet: Probe specificity. Standard NESA reactions were performed using 0.2 fmol of genomic DNA from various Bacillus species. No cut probe was detected in the absence of added DNA or using the Bsub 6 probe with B. anthracis, B. cereus or B thuringiensis genomic DNA. *, significantly different ( t -test) from the no added DNA control ( P

    Article Snippet: We used two 21-mer B. subtilis probes, Bsub 3 and Bsub 6, that have a standard T m of 50.6 and 56.3°C, respectively (Integrated DNA Technologies Inc).

    Techniques: