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    New England Biolabs nebuffer2 new england biolabs
    Nebuffer2 New England Biolabs, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs new england biolabs buffer 2
    Determination of divalent cation cofactor requirement and DNA substrate preference for GmrSD digestion. (A). Metal ion cofactor requirement for GmrSD digestion. Divalent cations or EDTA are indicated on top of each lane. (B). Substrate preference and optimal substrate size for GmrSD digestion. PCR DNA substrates containing 5hmC or regular dC were generated by PCR using pBR322 template and digested by GmrSD endonuclease in the presence of 1 mM ATP in <t>NEB</t> buffer 2. The same DNA substrates were also digested by HpaII (CCGG) in NEB buffer 4 (to confirm modified DNA).
    New England Biolabs Buffer 2, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 152 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 152 article reviews
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    93
    New England Biolabs nebuffer 2
    Determination of divalent cation cofactor requirement and DNA substrate preference for GmrSD digestion. (A). Metal ion cofactor requirement for GmrSD digestion. Divalent cations or EDTA are indicated on top of each lane. (B). Substrate preference and optimal substrate size for GmrSD digestion. PCR DNA substrates containing 5hmC or regular dC were generated by PCR using pBR322 template and digested by GmrSD endonuclease in the presence of 1 mM ATP in <t>NEB</t> buffer 2. The same DNA substrates were also digested by HpaII (CCGG) in NEB buffer 4 (to confirm modified DNA).
    Nebuffer 2, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 2310 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 2310 article reviews
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    New England Biolabs digestion mixture
    Determination of divalent cation cofactor requirement and DNA substrate preference for GmrSD digestion. (A). Metal ion cofactor requirement for GmrSD digestion. Divalent cations or EDTA are indicated on top of each lane. (B). Substrate preference and optimal substrate size for GmrSD digestion. PCR DNA substrates containing 5hmC or regular dC were generated by PCR using pBR322 template and digested by GmrSD endonuclease in the presence of 1 mM ATP in <t>NEB</t> buffer 2. The same DNA substrates were also digested by HpaII (CCGG) in NEB buffer 4 (to confirm modified DNA).
    Digestion Mixture, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Determination of divalent cation cofactor requirement and DNA substrate preference for GmrSD digestion. (A). Metal ion cofactor requirement for GmrSD digestion. Divalent cations or EDTA are indicated on top of each lane. (B). Substrate preference and optimal substrate size for GmrSD digestion. PCR DNA substrates containing 5hmC or regular dC were generated by PCR using pBR322 template and digested by GmrSD endonuclease in the presence of 1 mM ATP in NEB buffer 2. The same DNA substrates were also digested by HpaII (CCGG) in NEB buffer 4 (to confirm modified DNA).

    Journal: Scientific Reports

    Article Title: Expression and purification of a single-chain Type IV restriction enzyme Eco94GmrSD and determination of its substrate preference

    doi: 10.1038/srep09747

    Figure Lengend Snippet: Determination of divalent cation cofactor requirement and DNA substrate preference for GmrSD digestion. (A). Metal ion cofactor requirement for GmrSD digestion. Divalent cations or EDTA are indicated on top of each lane. (B). Substrate preference and optimal substrate size for GmrSD digestion. PCR DNA substrates containing 5hmC or regular dC were generated by PCR using pBR322 template and digested by GmrSD endonuclease in the presence of 1 mM ATP in NEB buffer 2. The same DNA substrates were also digested by HpaII (CCGG) in NEB buffer 4 (to confirm modified DNA).

    Article Snippet: GmrSD enzyme activity assay T4 (glc-5hmC), T4gt (5hmC), and λ DNA (Dam+ Dcm+ ) or 5hmC-modified PCR DNA were digested with purified GmrSD enzyme in NEB buffer 2 (50 mM NaCl, 10 mM Tris-HCl, 10 mM MgCl2, 1 mM DTT) supplemented with 1 mM ATP at 37°C for 1 h unless specified otherwise.

    Techniques: Polymerase Chain Reaction, Generated, Modification