Journal: Cells

Article Title: Inhibition of Mitochondrial Complex Function—The Hepatotoxicity Mechanism of Emodin Based on Quantitative Proteomic Analyses

doi: 10.3390/cells8030263

Figure Lengend Snippet: The differentially expressed proteins involved in the oxidative phosphorylation pathway and respiratory chain complex.

Article Snippet: Caspase-8, caspase-3, Ndufs1, Cox7A2, ATP6, SDHA, and Bax inhibitor 1 antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA).

Techniques:

Journal: Nucleic Acids Research

Article Title: FARS2 deficiency in Drosophila reveals the developmental delay and seizure manifested by aberrant mitochondrial tRNA metabolism

doi: 10.1093/nar/gkab1187

Figure Lengend Snippet: Western blotting analysis of mitochondrial proteins. ( A ) Ten micrograms of total proteins from various larvae were electrophoresed through a denaturing polyacrylamide gel, electroblotted and hybridized with antibodies for 6 subunits of OXPHOS (mtDNA-encoded CO3, complex IV, nuclear-encoded UQRCFS1, complex III; NDUFS3 and NDUFS1, complex I; ATP5A, complex V; SDHA, complex II), and Porin as a loading control, respectively. ( B ) Quantification of relative mitochondrial protein levels. Average content of each polypeptide was normalized to the average content of Porin in each genotype. n = 3. Calculations were based on three independent experiments. Graph details and symbols are explained in the legend to Figure .

Article Snippet: The primary antibodies used include anti-NDUFS3 (1:1000; Abcam, ab14711), anti-UQCRFS1 (1:1000; Abcam, ab14746), anti-CO3 (1:1000; Abcam, ab110259), anti-NDUFS1 (1:1000; Proteintech, 12444-1-AP), anti-ATP5A (1:2000; Abcam, ab14748), anti-SDHA (1:1000; Abcam, ab209986), anti-TFAM (1:1000; BOSTER, ba2827) and anti-Porin (1:2000; Abcam, ab14734).

Techniques: Western Blot