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  • 99
    New England Biolabs ndei
    Ndei, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 5999 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ndei/product/New England Biolabs
    Average 99 stars, based on 5999 article reviews
    Price from $9.99 to $1999.99
    ndei - by Bioz Stars, 2020-12
    99/100 stars
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    99
    Thermo Fisher ndei
    Ndei, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2395 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ndei/product/Thermo Fisher
    Average 99 stars, based on 2395 article reviews
    Price from $9.99 to $1999.99
    ndei - by Bioz Stars, 2020-12
    99/100 stars
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    99
    Millipore ndei
    Ndei, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 14619 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ndei/product/Millipore
    Average 99 stars, based on 14619 article reviews
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    99
    New England Biolabs nde i
    Nde I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 3083 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nde i/product/New England Biolabs
    Average 99 stars, based on 3083 article reviews
    Price from $9.99 to $1999.99
    nde i - by Bioz Stars, 2020-12
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    ndei  (TaKaRa)
    99
    TaKaRa ndei
    Ndei, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1120 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ndei/product/TaKaRa
    Average 99 stars, based on 1120 article reviews
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    ndei - by Bioz Stars, 2020-12
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    98
    Promega ndei
    Linear plasmid DNA molecule abundance is higher when friction forces are applied. ( A ) Extraction of the plasmid <t>pET-19b</t> from sepiolite-treated E. coli DH5α, a recA deficient strain, after one minute of friction treatment (five extractions per treatment). Note the enrichment of linearised plasmid DNA molecules (5 Kb fragments?) from bacteria treated with sepiolite after two minutes of friction applied to a 1% agarose gel. ( B ) Box-plot of the abundance of single-strand DNA molecules after different experimental treatments (control, sepiolite without friction and sepiolite with friction). Plasmid pET-19b digested with a single cut site enzyme <t>NdeI</t> was used as a control for the linear molecule migration rate and as a reference to calculate relative intensities using a densitometry analysis. The asterisks represent a significant difference; Tukey HSD test: P
    Ndei, supplied by Promega, used in various techniques. Bioz Stars score: 98/100, based on 465 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ndei/product/Promega
    Average 98 stars, based on 465 article reviews
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    ndei - by Bioz Stars, 2020-12
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    99
    Millipore ndei hindiii
    Linear plasmid DNA molecule abundance is higher when friction forces are applied. ( A ) Extraction of the plasmid <t>pET-19b</t> from sepiolite-treated E. coli DH5α, a recA deficient strain, after one minute of friction treatment (five extractions per treatment). Note the enrichment of linearised plasmid DNA molecules (5 Kb fragments?) from bacteria treated with sepiolite after two minutes of friction applied to a 1% agarose gel. ( B ) Box-plot of the abundance of single-strand DNA molecules after different experimental treatments (control, sepiolite without friction and sepiolite with friction). Plasmid pET-19b digested with a single cut site enzyme <t>NdeI</t> was used as a control for the linear molecule migration rate and as a reference to calculate relative intensities using a densitometry analysis. The asterisks represent a significant difference; Tukey HSD test: P
    Ndei Hindiii, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 148 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ndei hindiii/product/Millipore
    Average 99 stars, based on 148 article reviews
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    ndei hindiii - by Bioz Stars, 2020-12
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    93
    Thermo Fisher fastdigest ndei
    Linear plasmid DNA molecule abundance is higher when friction forces are applied. ( A ) Extraction of the plasmid <t>pET-19b</t> from sepiolite-treated E. coli DH5α, a recA deficient strain, after one minute of friction treatment (five extractions per treatment). Note the enrichment of linearised plasmid DNA molecules (5 Kb fragments?) from bacteria treated with sepiolite after two minutes of friction applied to a 1% agarose gel. ( B ) Box-plot of the abundance of single-strand DNA molecules after different experimental treatments (control, sepiolite without friction and sepiolite with friction). Plasmid pET-19b digested with a single cut site enzyme <t>NdeI</t> was used as a control for the linear molecule migration rate and as a reference to calculate relative intensities using a densitometry analysis. The asterisks represent a significant difference; Tukey HSD test: P
    Fastdigest Ndei, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fastdigest ndei/product/Thermo Fisher
    Average 93 stars, based on 53 article reviews
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    fastdigest ndei - by Bioz Stars, 2020-12
    93/100 stars
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    99
    New England Biolabs restriction endonuclease ndei
    Linear plasmid DNA molecule abundance is higher when friction forces are applied. ( A ) Extraction of the plasmid <t>pET-19b</t> from sepiolite-treated E. coli DH5α, a recA deficient strain, after one minute of friction treatment (five extractions per treatment). Note the enrichment of linearised plasmid DNA molecules (5 Kb fragments?) from bacteria treated with sepiolite after two minutes of friction applied to a 1% agarose gel. ( B ) Box-plot of the abundance of single-strand DNA molecules after different experimental treatments (control, sepiolite without friction and sepiolite with friction). Plasmid pET-19b digested with a single cut site enzyme <t>NdeI</t> was used as a control for the linear molecule migration rate and as a reference to calculate relative intensities using a densitometry analysis. The asterisks represent a significant difference; Tukey HSD test: P
    Restriction Endonuclease Ndei, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction endonuclease ndei/product/New England Biolabs
    Average 99 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    restriction endonuclease ndei - by Bioz Stars, 2020-12
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    N/A
    Standard format Plasmid sent in bacteria as agar stab
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    N/A
    Standard format Plasmid sent in bacteria as agar stab
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    N/A
    Standard format Plasmid sent in bacteria as agar stab
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    Image Search Results


    Linear plasmid DNA molecule abundance is higher when friction forces are applied. ( A ) Extraction of the plasmid pET-19b from sepiolite-treated E. coli DH5α, a recA deficient strain, after one minute of friction treatment (five extractions per treatment). Note the enrichment of linearised plasmid DNA molecules (5 Kb fragments?) from bacteria treated with sepiolite after two minutes of friction applied to a 1% agarose gel. ( B ) Box-plot of the abundance of single-strand DNA molecules after different experimental treatments (control, sepiolite without friction and sepiolite with friction). Plasmid pET-19b digested with a single cut site enzyme NdeI was used as a control for the linear molecule migration rate and as a reference to calculate relative intensities using a densitometry analysis. The asterisks represent a significant difference; Tukey HSD test: P

    Journal: Scientific Reports

    Article Title: Clay-induced DNA breaks as a path for genetic diversity, antibiotic resistance, and asbestos carcinogenesis

    doi: 10.1038/s41598-018-26958-5

    Figure Lengend Snippet: Linear plasmid DNA molecule abundance is higher when friction forces are applied. ( A ) Extraction of the plasmid pET-19b from sepiolite-treated E. coli DH5α, a recA deficient strain, after one minute of friction treatment (five extractions per treatment). Note the enrichment of linearised plasmid DNA molecules (5 Kb fragments?) from bacteria treated with sepiolite after two minutes of friction applied to a 1% agarose gel. ( B ) Box-plot of the abundance of single-strand DNA molecules after different experimental treatments (control, sepiolite without friction and sepiolite with friction). Plasmid pET-19b digested with a single cut site enzyme NdeI was used as a control for the linear molecule migration rate and as a reference to calculate relative intensities using a densitometry analysis. The asterisks represent a significant difference; Tukey HSD test: P

    Article Snippet: A NdeI (Promega, USA) digested aliquot of pET-19b was used as a control for the linear migration rate.

    Techniques: Plasmid Preparation, Positron Emission Tomography, Agarose Gel Electrophoresis, Migration

    Confirmation of KH1 deletion in L. infantum . ( a ) Comparison of the copy number of KH1 gene in the genomic DNA of the mutant lines. The copy number was determined by quantitative real-time PCR relative to the housekeeping genes GAPDH , using the comparative C T method (2 −ΔΔCT Method). ( b ) Schematic representation of KH1 locus before and after the cassettes integration. The cleavage sites of Nde I and Pvu II restriction enzymes and the length of the generated fragments were represented. ( c , d ) Southern blot analysis performed with 10 µg of genomic DNA of L. infantum wild-type (WT), the mutants Δ Likh1 + , Δ Likh1 and Δ Likh1 [pSP72αZEOα/ KH1 ] were digested with Nde I and Pvu II restriction enzymes. The membranes were hybridized with 32P -labelled probes, that reconize the KH1 5′UTR ( c ) or Kh1 gene ( d ). The normalization was performed by calculating the KH1/GAPDH ratio. Similar results were obtained when normalizing with DNA polymerase as housekeeping gene. Exposures were cropped from original images available as supplementary material.

    Journal: Scientific Reports

    Article Title: Growth arrested live-attenuated Leishmania infantum KHARON1 null mutants display cytokinesis defect and protective immunity in mice

    doi: 10.1038/s41598-018-30076-7

    Figure Lengend Snippet: Confirmation of KH1 deletion in L. infantum . ( a ) Comparison of the copy number of KH1 gene in the genomic DNA of the mutant lines. The copy number was determined by quantitative real-time PCR relative to the housekeeping genes GAPDH , using the comparative C T method (2 −ΔΔCT Method). ( b ) Schematic representation of KH1 locus before and after the cassettes integration. The cleavage sites of Nde I and Pvu II restriction enzymes and the length of the generated fragments were represented. ( c , d ) Southern blot analysis performed with 10 µg of genomic DNA of L. infantum wild-type (WT), the mutants Δ Likh1 + , Δ Likh1 and Δ Likh1 [pSP72αZEOα/ KH1 ] were digested with Nde I and Pvu II restriction enzymes. The membranes were hybridized with 32P -labelled probes, that reconize the KH1 5′UTR ( c ) or Kh1 gene ( d ). The normalization was performed by calculating the KH1/GAPDH ratio. Similar results were obtained when normalizing with DNA polymerase as housekeeping gene. Exposures were cropped from original images available as supplementary material.

    Article Snippet: About 10 µg of genomic DNA from L. infantum WT, Δ Likh1 + , Δ Likh1 and Δ Likh1 [pSP72αZEOα/ KH1 ] were digested with restriction enzymes Pvu II and Nde I (Promega, Madison, WI, USA).

    Techniques: Mutagenesis, Real-time Polymerase Chain Reaction, Generated, Southern Blot