Journal: Translational research : the journal of laboratory and clinical medicine
Article Title: Nanoformulated Copper/Zinc Superoxide Dismutase Exerts Differential Effects on Glucose versus Lipid Homeostasis Depending on the Diet Composition Possibly via Altered AMPK Signaling
Figure Lengend Snippet: Effect of Nano on systemic insulin sensitivity, glucose disposal, and hepatic glucose output Mice were intraperitoneally injected with insulin for insulin tolerance test and blood glucose was recorded at indicated time points (A). Area under the curve glucose values from ITT were determined (B). GTT was performed by injecting an acute bolus of glucose and blood glucose was determined at various time points (C). Area under the curve glucose values from GTT were recorded (D). PTT was performed by injecting an acute bolus of sodium pyruvate and blood glucose was determined at various time points (E). Area under the curve glucose values from PTT were recorded (F). Blood glucose values for ITT, GTT, and PTT are plotted as percent change from baseline (0 min). Values are expressed as mean ± SEM of 6–8 mice in each group. Total RNA was extracted from liver and real-time PCR was carried out for genes involved in gluconeogenesis (G). Values are normalized to 18S RNA and are mean ± SEM of 6–8 samples per group. CD, Chow diet. HF, High fat, Nn, nanoSOD.
Article Snippet: RNA was quantified using a Nano-drop spectrophotometer and 200 ng RNA was transcribed using 5x iScript reaction mix and iScript reverse transcriptase (Bio-Rad, USA).
Techniques: Mouse Assay, Injection, Real-time Polymerase Chain Reaction