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    Thermo Fisher nd 1000 spectrophotometer
    Analysis of DDZ-AuNP sensitivity. A) DDZ-M-AuNP colorimetric assays were performed on DENV-2 NGC titers of 1 × 10 1 , 1 × 10 2 , 1 × 10 4 , and 1 × 10 6 to determine the limits of detection. Samples were assembled as described in Methods, incubated at 37°C for 30 minutes and photographed. Results show that the DDZ-M-AuNP colorimetric assay is capable of detecting DENV-2 at a titer as low as 1 × 10 1 . DDZ-M = anti-dengue virus DNAzyme, DDZin-M = inactive anti-dengue virus DNAzyme. B) Analysis of DDZ-AuNP sensitivity by UV/Vis spectrophotometry. Ten microliters (10 uL) of cell culture fluid from Mock, DENV-2 NGC (titers of 1 × 10 1 , 1 × 10 2 1 × 10 6 /mL), or CHIKV vaccine strain 181/25 (1 × 10 6/ mL) infected C6/36 cells, or DENV serially diluted from 1 × 10 1 (Dil1 through Dil5) or were added to separate mixtures as described in A) . UV/Vis spectrophotometric analysis was performed using the <t>ND-1000</t> spectrophotometer at an absorbance of 520 nm. Absorbance measurements were graphed in log scale to illustrate sensitivity and accuracy of the colorimetric DENV detection method.
    Nd 1000 Spectrophotometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 57123 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nd 1000 spectrophotometer/product/Thermo Fisher
    Average 99 stars, based on 57123 article reviews
    Price from $9.99 to $1999.99
    nd 1000 spectrophotometer - by Bioz Stars, 2019-08
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    99
    Thermo Fisher nd 1000 uv spectrophotometer
    DNA yield of dust isolated from swine confinement facilities, grain elevators and households without pets. Total genomic DNA was isolated by a bead-beating protocol (Mo Bio, Power Clean, Carlsbad, CA) and quantified using a Nanodrop <t>ND-1000</t> UV spectrophotometer (NanoDrop Technologies, Wilmington, DE). Each bar represents the mean DNA yield (µg/mg dust) ± SEM from two independent dust samples.
    Nd 1000 Uv Spectrophotometer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1252 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nd 1000 uv spectrophotometer/product/Thermo Fisher
    Average 99 stars, based on 1252 article reviews
    Price from $9.99 to $1999.99
    nd 1000 uv spectrophotometer - by Bioz Stars, 2019-08
    99/100 stars
      Buy from Supplier

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    Analysis of DDZ-AuNP sensitivity. A) DDZ-M-AuNP colorimetric assays were performed on DENV-2 NGC titers of 1 × 10 1 , 1 × 10 2 , 1 × 10 4 , and 1 × 10 6 to determine the limits of detection. Samples were assembled as described in Methods, incubated at 37°C for 30 minutes and photographed. Results show that the DDZ-M-AuNP colorimetric assay is capable of detecting DENV-2 at a titer as low as 1 × 10 1 . DDZ-M = anti-dengue virus DNAzyme, DDZin-M = inactive anti-dengue virus DNAzyme. B) Analysis of DDZ-AuNP sensitivity by UV/Vis spectrophotometry. Ten microliters (10 uL) of cell culture fluid from Mock, DENV-2 NGC (titers of 1 × 10 1 , 1 × 10 2 1 × 10 6 /mL), or CHIKV vaccine strain 181/25 (1 × 10 6/ mL) infected C6/36 cells, or DENV serially diluted from 1 × 10 1 (Dil1 through Dil5) or were added to separate mixtures as described in A) . UV/Vis spectrophotometric analysis was performed using the ND-1000 spectrophotometer at an absorbance of 520 nm. Absorbance measurements were graphed in log scale to illustrate sensitivity and accuracy of the colorimetric DENV detection method.

    Journal: Virology Journal

    Article Title: A novel dengue virus detection method that couples DNAzyme and gold nanoparticle approaches

    doi: 10.1186/1743-422X-10-201

    Figure Lengend Snippet: Analysis of DDZ-AuNP sensitivity. A) DDZ-M-AuNP colorimetric assays were performed on DENV-2 NGC titers of 1 × 10 1 , 1 × 10 2 , 1 × 10 4 , and 1 × 10 6 to determine the limits of detection. Samples were assembled as described in Methods, incubated at 37°C for 30 minutes and photographed. Results show that the DDZ-M-AuNP colorimetric assay is capable of detecting DENV-2 at a titer as low as 1 × 10 1 . DDZ-M = anti-dengue virus DNAzyme, DDZin-M = inactive anti-dengue virus DNAzyme. B) Analysis of DDZ-AuNP sensitivity by UV/Vis spectrophotometry. Ten microliters (10 uL) of cell culture fluid from Mock, DENV-2 NGC (titers of 1 × 10 1 , 1 × 10 2 1 × 10 6 /mL), or CHIKV vaccine strain 181/25 (1 × 10 6/ mL) infected C6/36 cells, or DENV serially diluted from 1 × 10 1 (Dil1 through Dil5) or were added to separate mixtures as described in A) . UV/Vis spectrophotometric analysis was performed using the ND-1000 spectrophotometer at an absorbance of 520 nm. Absorbance measurements were graphed in log scale to illustrate sensitivity and accuracy of the colorimetric DENV detection method.

    Article Snippet: Quantification of these oligonucleotides was performed with the ND-1000 spectrophotometer from NanoDrop (Wilmington, DE).

    Techniques: Incubation, Colorimetric Assay, Spectrophotometry, Cell Culture, Infection

    Assessment of DDZ-AuNP specificity. A) DENV-2 and CHIKV (1 × 106/mL each) were placed in a buffered solution containing 10 mM MgCl2, 1 × 105 DDZ-AuNP particles/mL, 1.5 M NaCl, and 0.5% (w/v) SDS. Eppendorf tubes containing these mixes were incubated at 37°C for 30 minutes and photographed. CHIKV = chikungunya virus. DENV-2 = dengue virus serotype 2, DDZ-M = anti-dengue virus DNAzyme, DDZin-M = inactive anti-dengue virus DNAzyme. B) Analysis of DENV detection by UV/Vis Spectrophotometry. Samples were assembled as was performed for in A, mixed, incubated at 37°C for 5 minutes, and spectrophotometric analysis was performed using the ND-1000 spectrophotometer. C) Detection of DENV by DDZ-M-AuNP in comparison to several other flaviviruses. The specificity of our DDZ-M-AuNP device to detect DENV and not other fellow flavivirus members YFV, JEV or ZV was determined as described earlier (see Figure 4 ). D) An alignment was performed on consensus sequences of each of the four DENV serotypes to determine the most optimal regions for the design of serotype specific DDZ-AuNP devices by determining the region of least conservation one serotype has with the other DENV serotypes. E) Colorimetric serotype-specific detection of DENV. Cell culture supernatants from C6/36 cells mock infected (Mock), or from cells infected with either DENV serotypes 1 through 4 or CHIKV (1 × 106/mL each) were placed in buffered solutions containing the necessary cofactors and AuNPs tethered with our all purpose DENV serotype specific DNAzyme, DDZ-M, or one of the serotype-specific DDZs (designated DDZ-1 through-4) designed to specifically target the corresponding DENV serotype (Table 1 ). Eppendorf tubes containing these mixes were incubated at 37°C for 5 minutes and photographed.

    Journal: Virology Journal

    Article Title: A novel dengue virus detection method that couples DNAzyme and gold nanoparticle approaches

    doi: 10.1186/1743-422X-10-201

    Figure Lengend Snippet: Assessment of DDZ-AuNP specificity. A) DENV-2 and CHIKV (1 × 106/mL each) were placed in a buffered solution containing 10 mM MgCl2, 1 × 105 DDZ-AuNP particles/mL, 1.5 M NaCl, and 0.5% (w/v) SDS. Eppendorf tubes containing these mixes were incubated at 37°C for 30 minutes and photographed. CHIKV = chikungunya virus. DENV-2 = dengue virus serotype 2, DDZ-M = anti-dengue virus DNAzyme, DDZin-M = inactive anti-dengue virus DNAzyme. B) Analysis of DENV detection by UV/Vis Spectrophotometry. Samples were assembled as was performed for in A, mixed, incubated at 37°C for 5 minutes, and spectrophotometric analysis was performed using the ND-1000 spectrophotometer. C) Detection of DENV by DDZ-M-AuNP in comparison to several other flaviviruses. The specificity of our DDZ-M-AuNP device to detect DENV and not other fellow flavivirus members YFV, JEV or ZV was determined as described earlier (see Figure 4 ). D) An alignment was performed on consensus sequences of each of the four DENV serotypes to determine the most optimal regions for the design of serotype specific DDZ-AuNP devices by determining the region of least conservation one serotype has with the other DENV serotypes. E) Colorimetric serotype-specific detection of DENV. Cell culture supernatants from C6/36 cells mock infected (Mock), or from cells infected with either DENV serotypes 1 through 4 or CHIKV (1 × 106/mL each) were placed in buffered solutions containing the necessary cofactors and AuNPs tethered with our all purpose DENV serotype specific DNAzyme, DDZ-M, or one of the serotype-specific DDZs (designated DDZ-1 through-4) designed to specifically target the corresponding DENV serotype (Table 1 ). Eppendorf tubes containing these mixes were incubated at 37°C for 5 minutes and photographed.

    Article Snippet: Quantification of these oligonucleotides was performed with the ND-1000 spectrophotometer from NanoDrop (Wilmington, DE).

    Techniques: Incubation, Spectrophotometry, Cell Culture, Infection

    Flowchart of study design to identify collection techniques that enable quantitative measures of taste gene expression. Samples were collected from 8 volunteers using the six different methods, the RNA was extracted and analysed with the NanoDrop ND-1000 Spectrophotometer (for quantity and purity) and Bio-analyser (analysis of RNA integrity). Real-time quantitative PCR was completed on taste tissue markers and taste genes, allowing for the identification of methods enabling quantitative measures of taste gene expression.

    Journal: PLoS ONE

    Article Title: A Comparison of Collection Techniques for Gene Expression Analysis of Human Oral Taste Tissue

    doi: 10.1371/journal.pone.0152157

    Figure Lengend Snippet: Flowchart of study design to identify collection techniques that enable quantitative measures of taste gene expression. Samples were collected from 8 volunteers using the six different methods, the RNA was extracted and analysed with the NanoDrop ND-1000 Spectrophotometer (for quantity and purity) and Bio-analyser (analysis of RNA integrity). Real-time quantitative PCR was completed on taste tissue markers and taste genes, allowing for the identification of methods enabling quantitative measures of taste gene expression.

    Article Snippet: Samples were assessed for RNA quantity using NanoDrop ND-1000 Spectrophotometer, with the purity determined by absorbance ratio measurement at 260nm/280nm (ideal ratio of 2.0).

    Techniques: Expressing, Spectrophotometry, Real-time Polymerase Chain Reaction

    (A) Microfluidic analyses of total RNA prepared from different life stages of Nylanderia pubens by the Trizol method. RNA standards and their corresponding sizes are provided in the left-most column. (B) PCR of a portion of the N. pubens ubiquin gene. DNase-treated RNA (from panel A) of larvae (lane 1 ) and adults (lane 2) was reverse transcribed and subsequently amplified by PCR. MW = molecular weight markers. Sizes shown on the left. (C) Results of spectrophotometric analysis of the RNA preparations from workers and larvae from the upper panel on an ND-1000 spectrophotometer. Standard deviation values are indicated by error bars. The mean 260:280 nm ratio is provided for each life stage within the bar. High quality figures are available online.

    Journal: Journal of Insect Science

    Article Title: Non-Enzymatic Hydrolysis of RNA in Workers of the Ant Nylanderia pubens

    doi: 10.1673/031.012.14601

    Figure Lengend Snippet: (A) Microfluidic analyses of total RNA prepared from different life stages of Nylanderia pubens by the Trizol method. RNA standards and their corresponding sizes are provided in the left-most column. (B) PCR of a portion of the N. pubens ubiquin gene. DNase-treated RNA (from panel A) of larvae (lane 1 ) and adults (lane 2) was reverse transcribed and subsequently amplified by PCR. MW = molecular weight markers. Sizes shown on the left. (C) Results of spectrophotometric analysis of the RNA preparations from workers and larvae from the upper panel on an ND-1000 spectrophotometer. Standard deviation values are indicated by error bars. The mean 260:280 nm ratio is provided for each life stage within the bar. High quality figures are available online.

    Article Snippet: For comparison, RNA quality and quantity were determined spectrophotometrically on an ND-1000 spectrophotometer (Nanodrop Technologies, Inc., http://www.nanodrop.com/ ).

    Techniques: Polymerase Chain Reaction, Amplification, Molecular Weight, Spectrophotometry, Standard Deviation

    DNA yield of dust isolated from swine confinement facilities, grain elevators and households without pets. Total genomic DNA was isolated by a bead-beating protocol (Mo Bio, Power Clean, Carlsbad, CA) and quantified using a Nanodrop ND-1000 UV spectrophotometer (NanoDrop Technologies, Wilmington, DE). Each bar represents the mean DNA yield (µg/mg dust) ± SEM from two independent dust samples.

    Journal: PLoS ONE

    Article Title: Shotgun Pyrosequencing Metagenomic Analyses of Dusts from Swine Confinement and Grain Facilities

    doi: 10.1371/journal.pone.0095578

    Figure Lengend Snippet: DNA yield of dust isolated from swine confinement facilities, grain elevators and households without pets. Total genomic DNA was isolated by a bead-beating protocol (Mo Bio, Power Clean, Carlsbad, CA) and quantified using a Nanodrop ND-1000 UV spectrophotometer (NanoDrop Technologies, Wilmington, DE). Each bar represents the mean DNA yield (µg/mg dust) ± SEM from two independent dust samples.

    Article Snippet: Total genomic DNA was isolated by bead beating following the manufacturer’s instructions (Mo Bio, PowerSoil Kit, Carlsbad, CA), then assayed using a Nanodrop ND-1000 UV spectrophotometer (NanoDrop Technologies, Wilmington, DE).

    Techniques: Isolation, Spectrophotometry