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    StemCultures ncx3
    Effect of ziram treatment on mitochondrial membrane potential and intracellular Ca 2+ levels in BHK-WT and in <t>BHK-NCX3</t> cells. (A) Representative confocal images of BHK-WT and BHK-NCX3 cells double stained with 20 nM TMRE and 5 μM Oregon Green BAPTA-1 after 24 h treatment with 0.1 μM DMSO (top panels of each experimental group) or 1 μM ziram (middle panels of each experimental group) and after 2 h treatment with 10 μM ziram (bottom panels of each experimental group). (B) Quantification of mitochondrial dysfunction expressed as TMRE arbitrary units in control conditions (DMSO-24 h), after 24 h with 1μM ziram or 2 h with 10 μM ziram in BHK-WT (black bars) and BHK-NCX3 (gray bars) cells. **P
    Ncx3, supplied by StemCultures, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Alpha Diagnostics ncx3
    <t>NCX3</t> knockdown reduced [Ca 2+ ] r and the calcium response elicited by high potassium depolarization in MHS myotubes. Myoblasts isolated from MHN and MHS were differentiated for 1 day and then transfected with either scramble- or NCX3-siRNA for 48 h. A,
    Ncx3, supplied by Alpha Diagnostics, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Swant anti ncx3
    Effect of <t>ncx3</t> genetic knocked-out on preconditioning-induced neuroprotection. A On top of the figure are included representative rostral-caudal brain sections stained with cresyl violet of preconditioned hypoxic-ischemic (wild-type) and preconditioned hypoxic-ischemic (ncx3−/−) mice. Brain damage induced in mice was evaluated as % of ischemic damage at P11. Data are expressed as mean ± SD ( n = 5–7). * P
    Anti Ncx3, supplied by Swant, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ncx3/product/Swant
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    ncx3  (Qiagen)
    90
    Qiagen ncx3
    Effect of <t>ncx3</t> genetic knocked-out on preconditioning-induced neuroprotection. A On top of the figure are included representative rostral-caudal brain sections stained with cresyl violet of preconditioned hypoxic-ischemic (wild-type) and preconditioned hypoxic-ischemic (ncx3−/−) mice. Brain damage induced in mice was evaluated as % of ischemic damage at P11. Data are expressed as mean ± SD ( n = 5–7). * P
    Ncx3, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ncx3/product/Qiagen
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ncx3 - by Bioz Stars, 2022-09
    90/100 stars
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    Image Search Results


    Effect of ziram treatment on mitochondrial membrane potential and intracellular Ca 2+ levels in BHK-WT and in BHK-NCX3 cells. (A) Representative confocal images of BHK-WT and BHK-NCX3 cells double stained with 20 nM TMRE and 5 μM Oregon Green BAPTA-1 after 24 h treatment with 0.1 μM DMSO (top panels of each experimental group) or 1 μM ziram (middle panels of each experimental group) and after 2 h treatment with 10 μM ziram (bottom panels of each experimental group). (B) Quantification of mitochondrial dysfunction expressed as TMRE arbitrary units in control conditions (DMSO-24 h), after 24 h with 1μM ziram or 2 h with 10 μM ziram in BHK-WT (black bars) and BHK-NCX3 (gray bars) cells. **P

    Journal: Neurotoxicology

    Article Title: Involvement of the Sodium-Calcium exchanger 3 (NCX3) in ziram-induced calcium dysregulation and toxicity

    doi: 10.1016/j.neuro.2014.09.004

    Figure Lengend Snippet: Effect of ziram treatment on mitochondrial membrane potential and intracellular Ca 2+ levels in BHK-WT and in BHK-NCX3 cells. (A) Representative confocal images of BHK-WT and BHK-NCX3 cells double stained with 20 nM TMRE and 5 μM Oregon Green BAPTA-1 after 24 h treatment with 0.1 μM DMSO (top panels of each experimental group) or 1 μM ziram (middle panels of each experimental group) and after 2 h treatment with 10 μM ziram (bottom panels of each experimental group). (B) Quantification of mitochondrial dysfunction expressed as TMRE arbitrary units in control conditions (DMSO-24 h), after 24 h with 1μM ziram or 2 h with 10 μM ziram in BHK-WT (black bars) and BHK-NCX3 (gray bars) cells. **P

    Article Snippet: Further examinations are needed to determine whether the cascade of events involving E1, ALDH and NCX3 is sequential, synergistic or independent and to explain why neurons lacking NCX3 are not completely protected from ziram-induced damage.

    Techniques: Staining

    Effect of ziram on cell morphology following treatment with 10 μM ziram. (A–B) Representative dot plots of BHK-WT cells following 2 h treatment with DMSO (vehicle control) or 10 μM ziram. (C–D) Representative dot plots of BHK-NCX3 cells following 2 h treatment with DMSO (vehicle control) or 10 μM ziram. (E) Representative histogram of BHK-WT and BHK-NCX3 cells treated with DMSO (black and gray respectively) or 10 μM ziram (blue and red respectively).

    Journal: Neurotoxicology

    Article Title: Involvement of the Sodium-Calcium exchanger 3 (NCX3) in ziram-induced calcium dysregulation and toxicity

    doi: 10.1016/j.neuro.2014.09.004

    Figure Lengend Snippet: Effect of ziram on cell morphology following treatment with 10 μM ziram. (A–B) Representative dot plots of BHK-WT cells following 2 h treatment with DMSO (vehicle control) or 10 μM ziram. (C–D) Representative dot plots of BHK-NCX3 cells following 2 h treatment with DMSO (vehicle control) or 10 μM ziram. (E) Representative histogram of BHK-WT and BHK-NCX3 cells treated with DMSO (black and gray respectively) or 10 μM ziram (blue and red respectively).

    Article Snippet: Further examinations are needed to determine whether the cascade of events involving E1, ALDH and NCX3 is sequential, synergistic or independent and to explain why neurons lacking NCX3 are not completely protected from ziram-induced damage.

    Techniques:

    Flow cytometry analysis of BHK-WT and BHK-NCX3 cell viability and cell death following 2 h treatment with 10 μM ziram. (A–D) Representative density plots of BHK-WT (A and B) and BHK-NCX3 (C and D) cells labeled with calcein-AM. The forward scatter parameter (FCS) on the X-axis is proportional to size. (E) Representative flow cytometric histograms showing functional incorporation of calcein-AM in BHK-WT and BHK-NCX3 cells treated with DMSO (black and gray histograms respectively) or 10 μM ziram (blue and red histograms respectively). (F) Bar graph illustrating the % of calcein-AM positive cells in the upper right quadrant for BHK-WT cells treated with DMSO (black bars) or ziram (blue bars) and BHK-NCX3 cells treated with DMSO (grey bars) or ziram (red bars). (G–J) Representative density plots of BHK-WT (G and H) and BHK-NCX3 (I and J) cells labeled with PI. (K) Representative flow cytometric histograms showing incorporation of PI in nonviable BHK-WT and BHK-NCX3 cells treated with DMSO (black and gray histograms respectively) or 10 μM ziram (blue and red histograms respectively). (F) Bar graph illustrating the % of PI positive cells in the upper left quadrant for BHK-WT cells treated with DMSO (black bars) or ziram (blue bars) and BHK-NCX3 cells treated with DMSO (grey bars) or ziram (red bars). Each bar represents the mean (±S.E.M.) of 2,500 cells studied in three independent experimental sessions. *P

    Journal: Neurotoxicology

    Article Title: Involvement of the Sodium-Calcium exchanger 3 (NCX3) in ziram-induced calcium dysregulation and toxicity

    doi: 10.1016/j.neuro.2014.09.004

    Figure Lengend Snippet: Flow cytometry analysis of BHK-WT and BHK-NCX3 cell viability and cell death following 2 h treatment with 10 μM ziram. (A–D) Representative density plots of BHK-WT (A and B) and BHK-NCX3 (C and D) cells labeled with calcein-AM. The forward scatter parameter (FCS) on the X-axis is proportional to size. (E) Representative flow cytometric histograms showing functional incorporation of calcein-AM in BHK-WT and BHK-NCX3 cells treated with DMSO (black and gray histograms respectively) or 10 μM ziram (blue and red histograms respectively). (F) Bar graph illustrating the % of calcein-AM positive cells in the upper right quadrant for BHK-WT cells treated with DMSO (black bars) or ziram (blue bars) and BHK-NCX3 cells treated with DMSO (grey bars) or ziram (red bars). (G–J) Representative density plots of BHK-WT (G and H) and BHK-NCX3 (I and J) cells labeled with PI. (K) Representative flow cytometric histograms showing incorporation of PI in nonviable BHK-WT and BHK-NCX3 cells treated with DMSO (black and gray histograms respectively) or 10 μM ziram (blue and red histograms respectively). (F) Bar graph illustrating the % of PI positive cells in the upper left quadrant for BHK-WT cells treated with DMSO (black bars) or ziram (blue bars) and BHK-NCX3 cells treated with DMSO (grey bars) or ziram (red bars). Each bar represents the mean (±S.E.M.) of 2,500 cells studied in three independent experimental sessions. *P

    Article Snippet: Further examinations are needed to determine whether the cascade of events involving E1, ALDH and NCX3 is sequential, synergistic or independent and to explain why neurons lacking NCX3 are not completely protected from ziram-induced damage.

    Techniques: Flow Cytometry, Cytometry, Labeling, Functional Assay

    Ziram-induced increase of intracellular Ca 2+ in BHK-NCX3 cells. (A) Effects of ziram on [Ca 2+ i ] when BHK-WT and BHK-NCX3 cells were perfused in normal Krebs (NCX3 forward mode of exchange). Superimposed traces of the effects of ziram on [Ca 2+ i ] when BHK cells were incubated in normal Krebs or in the presence of 10 μM KBR-7943 (KBR) (B) Effect of Na + -free solution on NCX3 reverse activity in BHK-WT and in BHK-NCX3 cells. Superimposed traces of the effect of Na + -free on [Ca 2+ i ] when BHK cells were perfused in Na+-free solution alone, or pre-incubated with 10μM ziram, 10 μM KB-R7943 (KBR) or 10 μM KB-R7943 + 10 μM ziram (KBR+ziram).

    Journal: Neurotoxicology

    Article Title: Involvement of the Sodium-Calcium exchanger 3 (NCX3) in ziram-induced calcium dysregulation and toxicity

    doi: 10.1016/j.neuro.2014.09.004

    Figure Lengend Snippet: Ziram-induced increase of intracellular Ca 2+ in BHK-NCX3 cells. (A) Effects of ziram on [Ca 2+ i ] when BHK-WT and BHK-NCX3 cells were perfused in normal Krebs (NCX3 forward mode of exchange). Superimposed traces of the effects of ziram on [Ca 2+ i ] when BHK cells were incubated in normal Krebs or in the presence of 10 μM KBR-7943 (KBR) (B) Effect of Na + -free solution on NCX3 reverse activity in BHK-WT and in BHK-NCX3 cells. Superimposed traces of the effect of Na + -free on [Ca 2+ i ] when BHK cells were perfused in Na+-free solution alone, or pre-incubated with 10μM ziram, 10 μM KB-R7943 (KBR) or 10 μM KB-R7943 + 10 μM ziram (KBR+ziram).

    Article Snippet: Further examinations are needed to determine whether the cascade of events involving E1, ALDH and NCX3 is sequential, synergistic or independent and to explain why neurons lacking NCX3 are not completely protected from ziram-induced damage.

    Techniques: Incubation, Activity Assay

    Ziram-induced neurotoxicity in primary mesencephalic cultures. (A) Immunoblots showing that NCX3 is expressed in wild-type brainstem (C57/Bl6) and absent in NCX3 −/− homogenate. Loading control detected with α-tubulin is shown. (B) TH and NeuN cell viability was normalized to the condition in DMSO (i.e. vehicle control) for each independent cell culture and expressed as % of vehicle control. TH + /NeuN + was calculated as the ratio of TH + to NeuN + cell viability normalized to vehicle control. (C) Representative photomicrographs of TH- (red) and NeuN-stained (green) mesencephalic cultures isolated from C57/Bl6 (top panels) and NCX3 −/− (bottom panels) mice. Left panels: control treatment. Right panels: ziram 1 μM. Ziram-induced TH cell damage was quantified by TH + /NeuN + immunohistochemistry. A minimum of 4 coverslips were analyzed for each treatment in three independent experiments.

    Journal: Neurotoxicology

    Article Title: Involvement of the Sodium-Calcium exchanger 3 (NCX3) in ziram-induced calcium dysregulation and toxicity

    doi: 10.1016/j.neuro.2014.09.004

    Figure Lengend Snippet: Ziram-induced neurotoxicity in primary mesencephalic cultures. (A) Immunoblots showing that NCX3 is expressed in wild-type brainstem (C57/Bl6) and absent in NCX3 −/− homogenate. Loading control detected with α-tubulin is shown. (B) TH and NeuN cell viability was normalized to the condition in DMSO (i.e. vehicle control) for each independent cell culture and expressed as % of vehicle control. TH + /NeuN + was calculated as the ratio of TH + to NeuN + cell viability normalized to vehicle control. (C) Representative photomicrographs of TH- (red) and NeuN-stained (green) mesencephalic cultures isolated from C57/Bl6 (top panels) and NCX3 −/− (bottom panels) mice. Left panels: control treatment. Right panels: ziram 1 μM. Ziram-induced TH cell damage was quantified by TH + /NeuN + immunohistochemistry. A minimum of 4 coverslips were analyzed for each treatment in three independent experiments.

    Article Snippet: Further examinations are needed to determine whether the cascade of events involving E1, ALDH and NCX3 is sequential, synergistic or independent and to explain why neurons lacking NCX3 are not completely protected from ziram-induced damage.

    Techniques: Western Blot, Cell Culture, Staining, Isolation, Mouse Assay, Immunohistochemistry

    Effect of ziram treatment on cell viability in BHK-WT and BHK-NCX3 cells. (A). Representative fluorescent images of BHK-WT and BHK-NCX3 cells double stained with calcein-AM and propidium iodide in control conditions (left panels), following 24 h treatment with ziram 1 μM (middle panels) and following 2 h treatment with 10 μM ziram (right panels). (B). Bar graph illustrating cell death in BHK-WT and BHK-NCX3 cells following ziram exposure and in control condition (DMSO-24h). ** P

    Journal: Neurotoxicology

    Article Title: Involvement of the Sodium-Calcium exchanger 3 (NCX3) in ziram-induced calcium dysregulation and toxicity

    doi: 10.1016/j.neuro.2014.09.004

    Figure Lengend Snippet: Effect of ziram treatment on cell viability in BHK-WT and BHK-NCX3 cells. (A). Representative fluorescent images of BHK-WT and BHK-NCX3 cells double stained with calcein-AM and propidium iodide in control conditions (left panels), following 24 h treatment with ziram 1 μM (middle panels) and following 2 h treatment with 10 μM ziram (right panels). (B). Bar graph illustrating cell death in BHK-WT and BHK-NCX3 cells following ziram exposure and in control condition (DMSO-24h). ** P

    Article Snippet: Further examinations are needed to determine whether the cascade of events involving E1, ALDH and NCX3 is sequential, synergistic or independent and to explain why neurons lacking NCX3 are not completely protected from ziram-induced damage.

    Techniques: Staining

    NCX3 knockdown reduced [Ca 2+ ] r and the calcium response elicited by high potassium depolarization in MHS myotubes. Myoblasts isolated from MHN and MHS were differentiated for 1 day and then transfected with either scramble- or NCX3-siRNA for 48 h. A,

    Journal: The Journal of Biological Chemistry

    Article Title: Ca2+ Influx via the Na+/Ca2+ Exchanger Is Enhanced in Malignant Hyperthermia Skeletal Muscle *

    doi: 10.1074/jbc.M114.550764

    Figure Lengend Snippet: NCX3 knockdown reduced [Ca 2+ ] r and the calcium response elicited by high potassium depolarization in MHS myotubes. Myoblasts isolated from MHN and MHS were differentiated for 1 day and then transfected with either scramble- or NCX3-siRNA for 48 h. A,

    Article Snippet: To assess contribution of the NCX3 in the Ca2+ response to high [K+ ]e , MHN and MHS myotubes were transfected with NCX3 or scramble-siRNA for 48 h and then loaded with Fura-2AM.

    Techniques: Isolation, Transfection

    NCX3 expression in MHN and MHS skeletal muscle cells. Quadriceps muscles were dissected from 3–5-month-old mice and cultured differentiated myotubes from MHN and MHS mice were homogenized and NCX3 protein levels were assessed by Western blot and

    Journal: The Journal of Biological Chemistry

    Article Title: Ca2+ Influx via the Na+/Ca2+ Exchanger Is Enhanced in Malignant Hyperthermia Skeletal Muscle *

    doi: 10.1074/jbc.M114.550764

    Figure Lengend Snippet: NCX3 expression in MHN and MHS skeletal muscle cells. Quadriceps muscles were dissected from 3–5-month-old mice and cultured differentiated myotubes from MHN and MHS mice were homogenized and NCX3 protein levels were assessed by Western blot and

    Article Snippet: To assess contribution of the NCX3 in the Ca2+ response to high [K+ ]e , MHN and MHS myotubes were transfected with NCX3 or scramble-siRNA for 48 h and then loaded with Fura-2AM.

    Techniques: Expressing, Mouse Assay, Cell Culture, Western Blot

    Effect of ncx3 genetic knocked-out on preconditioning-induced neuroprotection. A On top of the figure are included representative rostral-caudal brain sections stained with cresyl violet of preconditioned hypoxic-ischemic (wild-type) and preconditioned hypoxic-ischemic (ncx3−/−) mice. Brain damage induced in mice was evaluated as % of ischemic damage at P11. Data are expressed as mean ± SD ( n = 5–7). * P

    Journal: Cell Death Discovery

    Article Title: Preconditioning in hypoxic-ischemic neonate mice triggers Na+-Ca2+ exchanger-dependent neurogenesis

    doi: 10.1038/s41420-022-01089-z

    Figure Lengend Snippet: Effect of ncx3 genetic knocked-out on preconditioning-induced neuroprotection. A On top of the figure are included representative rostral-caudal brain sections stained with cresyl violet of preconditioned hypoxic-ischemic (wild-type) and preconditioned hypoxic-ischemic (ncx3−/−) mice. Brain damage induced in mice was evaluated as % of ischemic damage at P11. Data are expressed as mean ± SD ( n = 5–7). * P

    Article Snippet: For DAB staining, standard 3,3-diaminobenzidine (DAB) was employed on coronal sections using anti-NCX1 (1:500) and anti-NCX3 (1:3000) (Swant, Bellinzona, Switzerland; catalog 95,209) [ ].

    Techniques: Staining, Mouse Assay

    Effect of NCX1 silencing and ncx3 genetic knocked-out on the differentiation induced by HPC. A Representative confocal images of dentate gyrus of preconditioned hypoxic-ischemic mice (siControl) and preconditioned hypoxic-ischemic mice (siNCX1). Single staining of p57 (A, B), NeuroD1 (C, D), Hoechst (E, F), and merge (G, H). Scale bar 75 μm. Arrows indicate p57 and NeuroD1 expressing cells. B Cell-counting analysis of total number of onlyp57 + cells in DG of P11 mice. C Cell-counting analysis of total number of only NeuroD1 + cells in DG of P11 mice. D Cell-counting analysis of total number of p57 + /NeuroD1 + cells in DG of P11 mice. B , C , D Data are expressed as mean ± SD ( n = 3). C * P

    Journal: Cell Death Discovery

    Article Title: Preconditioning in hypoxic-ischemic neonate mice triggers Na+-Ca2+ exchanger-dependent neurogenesis

    doi: 10.1038/s41420-022-01089-z

    Figure Lengend Snippet: Effect of NCX1 silencing and ncx3 genetic knocked-out on the differentiation induced by HPC. A Representative confocal images of dentate gyrus of preconditioned hypoxic-ischemic mice (siControl) and preconditioned hypoxic-ischemic mice (siNCX1). Single staining of p57 (A, B), NeuroD1 (C, D), Hoechst (E, F), and merge (G, H). Scale bar 75 μm. Arrows indicate p57 and NeuroD1 expressing cells. B Cell-counting analysis of total number of onlyp57 + cells in DG of P11 mice. C Cell-counting analysis of total number of only NeuroD1 + cells in DG of P11 mice. D Cell-counting analysis of total number of p57 + /NeuroD1 + cells in DG of P11 mice. B , C , D Data are expressed as mean ± SD ( n = 3). C * P

    Article Snippet: For DAB staining, standard 3,3-diaminobenzidine (DAB) was employed on coronal sections using anti-NCX1 (1:500) and anti-NCX3 (1:3000) (Swant, Bellinzona, Switzerland; catalog 95,209) [ ].

    Techniques: Mouse Assay, Staining, Expressing, Cell Counting

    NCX1 and NCX3 expression in the dentate gyrus after HPC. A Representative image of NCX1 3,3′-diaminobenzidine (DAB) staining of dentate gyrus of control, HI, and PC + HI groups. Scale bar 25 μm. Arrows indicate NCX1 expressing cells. Cell-counting analysis was expressed as the percentage of total number of NCX1 + cells in dentate gyrus of P11 mice. B Representative image of NCX3 DAB staining of dentate gyrus of control, HI, and PC + HI groups. Scale bar 25 μm. Arrows indicate NCX3 expressing cells. Cell-counting analysis was expressed as the percentage of total number of NCX3 + cells in dentate gyrus of P11 mice. A , B Data are expressed as mean ± SD ( n = 3). * P

    Journal: Cell Death Discovery

    Article Title: Preconditioning in hypoxic-ischemic neonate mice triggers Na+-Ca2+ exchanger-dependent neurogenesis

    doi: 10.1038/s41420-022-01089-z

    Figure Lengend Snippet: NCX1 and NCX3 expression in the dentate gyrus after HPC. A Representative image of NCX1 3,3′-diaminobenzidine (DAB) staining of dentate gyrus of control, HI, and PC + HI groups. Scale bar 25 μm. Arrows indicate NCX1 expressing cells. Cell-counting analysis was expressed as the percentage of total number of NCX1 + cells in dentate gyrus of P11 mice. B Representative image of NCX3 DAB staining of dentate gyrus of control, HI, and PC + HI groups. Scale bar 25 μm. Arrows indicate NCX3 expressing cells. Cell-counting analysis was expressed as the percentage of total number of NCX3 + cells in dentate gyrus of P11 mice. A , B Data are expressed as mean ± SD ( n = 3). * P

    Article Snippet: For DAB staining, standard 3,3-diaminobenzidine (DAB) was employed on coronal sections using anti-NCX1 (1:500) and anti-NCX3 (1:3000) (Swant, Bellinzona, Switzerland; catalog 95,209) [ ].

    Techniques: Expressing, Staining, Cell Counting, Mouse Assay