ncode mirna first-strand cdna synthesis Thermo Fisher Search Results


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    Thermo Fisher ncode mirna first strand cdna synthesis kit
    <t>miRNA</t> and gene expression in calvarial cells from Runx2−/− embryos. after transfected with overexpression vector for Dicer or empty vector. (A) Runx2−/− cells were transfected with Dicer <t>cDNA</t> plasmids or empty vector. Immunohistochemical analysis was performed to confirm increased expression of DICER in Dicer cDNA group compared to with empty vector control (intense red signals, x200). (B) miRNA and gene expressions were detected by qRT-PCR in Runx2−/− cells transfected with DICER cDNAs or empty vector. These data are expressed as the mean ± SD (n=3). * p
    Ncode Mirna First Strand Cdna Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4777 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    miRNA and gene expression in calvarial cells from Runx2−/− embryos. after transfected with overexpression vector for Dicer or empty vector. (A) Runx2−/− cells were transfected with Dicer cDNA plasmids or empty vector. Immunohistochemical analysis was performed to confirm increased expression of DICER in Dicer cDNA group compared to with empty vector control (intense red signals, x200). (B) miRNA and gene expressions were detected by qRT-PCR in Runx2−/− cells transfected with DICER cDNAs or empty vector. These data are expressed as the mean ± SD (n=3). * p

    Journal: Journal of cellular physiology

    Article Title: Runx2 /DICER/miRNA Pathway in Regulating Osteogenesis †

    doi: 10.1002/jcp.25406

    Figure Lengend Snippet: miRNA and gene expression in calvarial cells from Runx2−/− embryos. after transfected with overexpression vector for Dicer or empty vector. (A) Runx2−/− cells were transfected with Dicer cDNA plasmids or empty vector. Immunohistochemical analysis was performed to confirm increased expression of DICER in Dicer cDNA group compared to with empty vector control (intense red signals, x200). (B) miRNA and gene expressions were detected by qRT-PCR in Runx2−/− cells transfected with DICER cDNAs or empty vector. These data are expressed as the mean ± SD (n=3). * p

    Article Snippet: For miRNA analysis, total RNA was extracted using the miRNeasy Mini Kit (Qiagen), cDNA synthesis was performed with 1μg of total RNA, using an NCode miRNA First-Strand cDNA Synthesis Kit (Invitrogen) according to the manufacturer’s protocol. qRT- PCR was performed on a Bio-Rad iQ5 thermal cycler using an NCode Express SYBR GreenER miRNA qRT-PCR Kit (Invitrogen).

    Techniques: Expressing, Transfection, Over Expression, Plasmid Preparation, Immunohistochemistry, Quantitative RT-PCR

    Quantitative real-time polymerase chain reaction analysis of miRNAs differentially expressed in the intestinal mucosal layer (IML) of necrotic enteritis (NE)-induced Fayoumi chickens along with their top ranked target genes. cDNA templates for the amplification of miRNAs and genes were synthesized separately, and amplified with appropriate miRNA- and gene-specific primers. (A), (B) miRNAs and genes were amplified with cDNAs from the IML of NE-induced Fayoumi chicken line M5.1. (C), (D) miRNAs and genes were amplified with cDNAs from the IML of NE-induced Fayoumi chicken line M15.2. The expression of miRNAs/genes in NE-induced samples was calculated after normalizing with chicken snoRNA/glyceraldehyde 3-phosphate dehydrogenase expression. Statistical significance: * p

    Journal: Asian-Australasian Journal of Animal Sciences

    Article Title: Distribution and differential expression of microRNAs in the intestinal mucosal layer of necrotic enteritis induced Fayoumi chickens

    doi: 10.5713/ajas.16.0685

    Figure Lengend Snippet: Quantitative real-time polymerase chain reaction analysis of miRNAs differentially expressed in the intestinal mucosal layer (IML) of necrotic enteritis (NE)-induced Fayoumi chickens along with their top ranked target genes. cDNA templates for the amplification of miRNAs and genes were synthesized separately, and amplified with appropriate miRNA- and gene-specific primers. (A), (B) miRNAs and genes were amplified with cDNAs from the IML of NE-induced Fayoumi chicken line M5.1. (C), (D) miRNAs and genes were amplified with cDNAs from the IML of NE-induced Fayoumi chicken line M15.2. The expression of miRNAs/genes in NE-induced samples was calculated after normalizing with chicken snoRNA/glyceraldehyde 3-phosphate dehydrogenase expression. Statistical significance: * p

    Article Snippet: The cDNA for miRNA amplification was synthesized from the total RNA (2 μg) using the NCode miRNA First-Strand cDNA Synthesis and qRT-PCR Kit (Invitrogen, USA) according to the manufacturer’s protocols.

    Techniques: Real-time Polymerase Chain Reaction, Amplification, Synthesized, Expressing