Journal: Asian-Australasian Journal of Animal Sciences
Article Title: Distribution and differential expression of microRNAs in the intestinal mucosal layer of necrotic enteritis induced Fayoumi chickens
Figure Lengend Snippet: Quantitative real-time polymerase chain reaction analysis of miRNAs differentially expressed in the intestinal mucosal layer (IML) of necrotic enteritis (NE)-induced Fayoumi chickens along with their top ranked target genes. cDNA templates for the amplification of miRNAs and genes were synthesized separately, and amplified with appropriate miRNA- and gene-specific primers. (A), (B) miRNAs and genes were amplified with cDNAs from the IML of NE-induced Fayoumi chicken line M5.1. (C), (D) miRNAs and genes were amplified with cDNAs from the IML of NE-induced Fayoumi chicken line M15.2. The expression of miRNAs/genes in NE-induced samples was calculated after normalizing with chicken snoRNA/glyceraldehyde 3-phosphate dehydrogenase expression. Statistical significance: * p
Article Snippet: The cDNA for miRNA amplification was synthesized from the total RNA (2 μg) using the NCode miRNA First-Strand cDNA Synthesis and qRT-PCR Kit (Invitrogen, USA) according to the manufacturer’s protocols.
Techniques: Real-time Polymerase Chain Reaction, Amplification, Synthesized, Expressing