ncode mirna first strand cdna synthesis Thermo Fisher Search Results


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    Thermo Fisher ncode mirna first strand cdna synthesis kit
    <t>miRNA</t> and gene expression in calvarial cells from Runx2−/− embryos. after transfected with overexpression vector for Dicer or empty vector. (A) Runx2−/− cells were transfected with Dicer <t>cDNA</t> plasmids or empty vector. Immunohistochemical analysis was performed to confirm increased expression of DICER in Dicer cDNA group compared to with empty vector control (intense red signals, x200). (B) miRNA and gene expressions were detected by qRT-PCR in Runx2−/− cells transfected with DICER cDNAs or empty vector. These data are expressed as the mean ± SD (n=3). * p
    Ncode Mirna First Strand Cdna Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 703 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ncode mirna first strand cdna synthesis kit/product/Thermo Fisher
    Average 90 stars, based on 703 article reviews
    Price from $9.99 to $1999.99
    ncode mirna first strand cdna synthesis kit - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher ncode mirna first strand cdna synthesis
    <t>miRNA</t> and gene expression in calvarial cells from Runx2−/− embryos. after transfected with overexpression vector for Dicer or empty vector. (A) Runx2−/− cells were transfected with Dicer <t>cDNA</t> plasmids or empty vector. Immunohistochemical analysis was performed to confirm increased expression of DICER in Dicer cDNA group compared to with empty vector control (intense red signals, x200). (B) miRNA and gene expressions were detected by qRT-PCR in Runx2−/− cells transfected with DICER cDNAs or empty vector. These data are expressed as the mean ± SD (n=3). * p
    Ncode Mirna First Strand Cdna Synthesis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 239 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ncode mirna first strand cdna synthesis/product/Thermo Fisher
    Average 90 stars, based on 239 article reviews
    Price from $9.99 to $1999.99
    ncode mirna first strand cdna synthesis - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

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    miRNA and gene expression in calvarial cells from Runx2−/− embryos. after transfected with overexpression vector for Dicer or empty vector. (A) Runx2−/− cells were transfected with Dicer cDNA plasmids or empty vector. Immunohistochemical analysis was performed to confirm increased expression of DICER in Dicer cDNA group compared to with empty vector control (intense red signals, x200). (B) miRNA and gene expressions were detected by qRT-PCR in Runx2−/− cells transfected with DICER cDNAs or empty vector. These data are expressed as the mean ± SD (n=3). * p

    Journal: Journal of cellular physiology

    Article Title: Runx2 /DICER/miRNA Pathway in Regulating Osteogenesis †

    doi: 10.1002/jcp.25406

    Figure Lengend Snippet: miRNA and gene expression in calvarial cells from Runx2−/− embryos. after transfected with overexpression vector for Dicer or empty vector. (A) Runx2−/− cells were transfected with Dicer cDNA plasmids or empty vector. Immunohistochemical analysis was performed to confirm increased expression of DICER in Dicer cDNA group compared to with empty vector control (intense red signals, x200). (B) miRNA and gene expressions were detected by qRT-PCR in Runx2−/− cells transfected with DICER cDNAs or empty vector. These data are expressed as the mean ± SD (n=3). * p

    Article Snippet: For miRNA analysis, total RNA was extracted using the miRNeasy Mini Kit (Qiagen), cDNA synthesis was performed with 1μg of total RNA, using an NCode miRNA First-Strand cDNA Synthesis Kit (Invitrogen) according to the manufacturer’s protocol. qRT- PCR was performed on a Bio-Rad iQ5 thermal cycler using an NCode Express SYBR GreenER miRNA qRT-PCR Kit (Invitrogen).

    Techniques: Expressing, Transfection, Over Expression, Plasmid Preparation, Immunohistochemistry, Quantitative RT-PCR