nci i Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    New England Biolabs ncii
    Targeted point mutation (851G→A, R284Q) of <t>Spata16</t> by CRISPR/Cas9. ( A ) Targeting scheme of the point mutation (851G→A, R284Q) in the fourth exon of mouse Spata16 . Spata16 consists of eleven exons. The point mutation was introduced into mouse ES cells using the HDR donor plasmid with 0.5 kb homology arms. Blue indicates the sgRNA sequence. Red indicates a G→A mutation at the last nucleotide (851st coding exon) of the fourth exon of Spata16 . Capital and small letters indicate nucleotides of exons and introns, respectively; ( B ) The genotyping of point-mutant mice by <t>Nci</t> I digestion. The Nci I recognition site (5′-CCCGG-3′) was disrupted due to the G→A mutation of the fourth exon. In wild-type mice, two bands (1372 bp and 1569 bp) were detected after Nci I digestion; ( C ) Direct sequencing of PCR products in Spata16 pm/pm mice. The G→A mutation at the last nucleotide (851st coding exon) of the fourth exon is indicated by the orange square; ( D ) RT-PCR analysis of a testis in Spata16 pm/pm mice. The 498-bp band was amplified from wild-type and Spata16 pm/pm mice. A point mutation causing an inappropriate splicing was not found in Spata16 pm/pm mouse testes; ( E ) Representative testicular histology sections stained with hematoxylin and eosin. Spermatogenesis in Spata16 pm/pm mice looked normal compared with that in Spata16 pm/wt mice. Scale bars: 100 μm; ( F ) Cauda epididymal spermatozoa from Spata16 pm/wt and Spata16 pm/pm mice. There were no sperm morphology differences between Spata16 pm/wt and Spata16 pm/pm mice. Spata16 pm/pm males were fertile. Scale bars: 50 μm.
    Ncii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ncii/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ncii - by Bioz Stars, 2022-08
    94/100 stars
      Buy from Supplier

    86
    New England Biolabs nci i
    a PCR amplification of nagt gene (1.4 kb) from Moroccan L. infantum strains. b , c , d PCR-amplified nagt digested with <t>Nci</t> I, Nae I, and Alw I, respectively. The numbers above and below the digested fragments indicate their sizes. Lanes 1–5: DNA samples of Moroccan L. infantum isolates 1 to 5; NC: Negative control; M: Molecular marker HyperLader™ 100 bp plus (Bioline, Taunton, MA, USA). e RFLP maps illustrating the nagt genotype identified in this work and those identified by Waki et al. [ 34 ]*. The vertical numbers indicate the cut position of each enzyme
    Nci I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nci i/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nci i - by Bioz Stars, 2022-08
    86/100 stars
      Buy from Supplier

    94
    Thermo Fisher bcni
    a PCR amplification of nagt gene (1.4 kb) from Moroccan L. infantum strains. b , c , d PCR-amplified nagt digested with <t>Nci</t> I, Nae I, and Alw I, respectively. The numbers above and below the digested fragments indicate their sizes. Lanes 1–5: DNA samples of Moroccan L. infantum isolates 1 to 5; NC: Negative control; M: Molecular marker HyperLader™ 100 bp plus (Bioline, Taunton, MA, USA). e RFLP maps illustrating the nagt genotype identified in this work and those identified by Waki et al. [ 34 ]*. The vertical numbers indicate the cut position of each enzyme
    Bcni, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bcni/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bcni - by Bioz Stars, 2022-08
    94/100 stars
      Buy from Supplier

    Image Search Results


    Targeted point mutation (851G→A, R284Q) of Spata16 by CRISPR/Cas9. ( A ) Targeting scheme of the point mutation (851G→A, R284Q) in the fourth exon of mouse Spata16 . Spata16 consists of eleven exons. The point mutation was introduced into mouse ES cells using the HDR donor plasmid with 0.5 kb homology arms. Blue indicates the sgRNA sequence. Red indicates a G→A mutation at the last nucleotide (851st coding exon) of the fourth exon of Spata16 . Capital and small letters indicate nucleotides of exons and introns, respectively; ( B ) The genotyping of point-mutant mice by Nci I digestion. The Nci I recognition site (5′-CCCGG-3′) was disrupted due to the G→A mutation of the fourth exon. In wild-type mice, two bands (1372 bp and 1569 bp) were detected after Nci I digestion; ( C ) Direct sequencing of PCR products in Spata16 pm/pm mice. The G→A mutation at the last nucleotide (851st coding exon) of the fourth exon is indicated by the orange square; ( D ) RT-PCR analysis of a testis in Spata16 pm/pm mice. The 498-bp band was amplified from wild-type and Spata16 pm/pm mice. A point mutation causing an inappropriate splicing was not found in Spata16 pm/pm mouse testes; ( E ) Representative testicular histology sections stained with hematoxylin and eosin. Spermatogenesis in Spata16 pm/pm mice looked normal compared with that in Spata16 pm/wt mice. Scale bars: 100 μm; ( F ) Cauda epididymal spermatozoa from Spata16 pm/wt and Spata16 pm/pm mice. There were no sperm morphology differences between Spata16 pm/wt and Spata16 pm/pm mice. Spata16 pm/pm males were fertile. Scale bars: 50 μm.

    Journal: International Journal of Molecular Sciences

    Article Title: Human Globozoospermia-Related Gene Spata16 Is Required for Sperm Formation Revealed by CRISPR/Cas9-Mediated Mouse Models

    doi: 10.3390/ijms18102208

    Figure Lengend Snippet: Targeted point mutation (851G→A, R284Q) of Spata16 by CRISPR/Cas9. ( A ) Targeting scheme of the point mutation (851G→A, R284Q) in the fourth exon of mouse Spata16 . Spata16 consists of eleven exons. The point mutation was introduced into mouse ES cells using the HDR donor plasmid with 0.5 kb homology arms. Blue indicates the sgRNA sequence. Red indicates a G→A mutation at the last nucleotide (851st coding exon) of the fourth exon of Spata16 . Capital and small letters indicate nucleotides of exons and introns, respectively; ( B ) The genotyping of point-mutant mice by Nci I digestion. The Nci I recognition site (5′-CCCGG-3′) was disrupted due to the G→A mutation of the fourth exon. In wild-type mice, two bands (1372 bp and 1569 bp) were detected after Nci I digestion; ( C ) Direct sequencing of PCR products in Spata16 pm/pm mice. The G→A mutation at the last nucleotide (851st coding exon) of the fourth exon is indicated by the orange square; ( D ) RT-PCR analysis of a testis in Spata16 pm/pm mice. The 498-bp band was amplified from wild-type and Spata16 pm/pm mice. A point mutation causing an inappropriate splicing was not found in Spata16 pm/pm mouse testes; ( E ) Representative testicular histology sections stained with hematoxylin and eosin. Spermatogenesis in Spata16 pm/pm mice looked normal compared with that in Spata16 pm/wt mice. Scale bars: 100 μm; ( F ) Cauda epididymal spermatozoa from Spata16 pm/wt and Spata16 pm/pm mice. There were no sperm morphology differences between Spata16 pm/wt and Spata16 pm/pm mice. Spata16 pm/pm males were fertile. Scale bars: 50 μm.

    Article Snippet: Spata16 point mutant mice were genotyped by Nci I restriction enzyme (New England Biolabs, Ipswich, MA, USA) digestion following PCR.

    Techniques: Mutagenesis, CRISPR, Plasmid Preparation, Sequencing, Mouse Assay, Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Amplification, Staining

    a PCR amplification of nagt gene (1.4 kb) from Moroccan L. infantum strains. b , c , d PCR-amplified nagt digested with Nci I, Nae I, and Alw I, respectively. The numbers above and below the digested fragments indicate their sizes. Lanes 1–5: DNA samples of Moroccan L. infantum isolates 1 to 5; NC: Negative control; M: Molecular marker HyperLader™ 100 bp plus (Bioline, Taunton, MA, USA). e RFLP maps illustrating the nagt genotype identified in this work and those identified by Waki et al. [ 34 ]*. The vertical numbers indicate the cut position of each enzyme

    Journal: Infectious Diseases of Poverty

    Article Title: Genetic polymorphism in Leishmania infantum isolates from human and animals determined by nagt PCR-RFLP

    doi: 10.1186/s40249-018-0439-y

    Figure Lengend Snippet: a PCR amplification of nagt gene (1.4 kb) from Moroccan L. infantum strains. b , c , d PCR-amplified nagt digested with Nci I, Nae I, and Alw I, respectively. The numbers above and below the digested fragments indicate their sizes. Lanes 1–5: DNA samples of Moroccan L. infantum isolates 1 to 5; NC: Negative control; M: Molecular marker HyperLader™ 100 bp plus (Bioline, Taunton, MA, USA). e RFLP maps illustrating the nagt genotype identified in this work and those identified by Waki et al. [ 34 ]*. The vertical numbers indicate the cut position of each enzyme

    Article Snippet: The thermocycler settings were an initial denaturation at 95 °C for 5 min, 30 cycles at 94 °C for 60 s, 58 °C for 60 s, and 72 °C for 90 s, and a final extension step at 72 °C for 5 min. Further RFLP analysis of the PCR-amplified nagt gene was performed separately using three restriction enzymes: Nae I, Alw I, and Nci I (New England Biolabs, Ipswich, MA, USA).

    Techniques: Polymerase Chain Reaction, Amplification, Negative Control, Marker