nb100-74435 Search Results


anti phospho histone h2a x ser139  (Novus Biologicals)
94
Novus Biologicals anti phospho histone h2a x ser139
(A) Measuring expression levels of phospho-ATR protein, phospho-Chk1 Ser317 to assess replication checkpoint activation using western blotting. Expression levels of H3 were used as a loading control and (B) DNA fiber analysis of 50uM hydroxyurea (HU) treated to assess replication fork stalling in pol eta −/− , PIP*, UBZ*, F1* and pol eta+ /+ cells. The fork rate (CldU/IdU ratio) is indicated, n=150. The p-values are indicated as follows: * <0.03, ** <0.0021, *** <0.0002, **** <0.0001. Scale bar 10 μm; (C) Analysis of number of gH2AX foci (red) in the nucleus per cell in Pol eta −/− , PIP*, UBZ*, F1* and Pol eta +/+ cells when exposed to Aphidicolin (Aph). Representative images are on the top, n=250; The p-values are indicated as follows: * <0.03, ** <0.0021, *** <0.0002, **** <0.0001. (D) Measurement of DNA single strand breaks by alkaline Comet assay in Pol eta −/− , PIP*, UBZ*, F1* and Pol eta +/+ cells treated with Aphidicolin (APH). The p-values are indicated as follows: * <0.03, ** <0.0021, *** <0.0002, **** <0.0001. Lengths were measured using the Adobe Photoshop Tools. (E) Time course experiment to measure recovery of cells after release into drug free media over the course of 48hours. Cells were collected at six time points (0, 4, 8, 12, 24, 28 hours) and expression levels of phospho-Histone H2AX <t>Ser139</t> were measured by Immunofluorescence staining. The p-values are indicated as follows: * <0.03, ** <0.0021, *** <0.0002, **** <0.0001. Scale bar 10 μm.
Anti Phospho Histone H2a X Ser139, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti phospho histone h2a x ser139/product/Novus Biologicals
Average 94 stars, based on 1 article reviews
anti phospho histone h2a x ser139 - by Bioz Stars, 2026-05
94/100 stars
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phosphorylated γh2ax  (Novus Biologicals)
94
Novus Biologicals phosphorylated γh2ax
(A) Measuring expression levels of phospho-ATR protein, phospho-Chk1 Ser317 to assess replication checkpoint activation using western blotting. Expression levels of H3 were used as a loading control and (B) DNA fiber analysis of 50uM hydroxyurea (HU) treated to assess replication fork stalling in pol eta −/− , PIP*, UBZ*, F1* and pol eta+ /+ cells. The fork rate (CldU/IdU ratio) is indicated, n=150. The p-values are indicated as follows: * <0.03, ** <0.0021, *** <0.0002, **** <0.0001. Scale bar 10 μm; (C) Analysis of number of gH2AX foci (red) in the nucleus per cell in Pol eta −/− , PIP*, UBZ*, F1* and Pol eta +/+ cells when exposed to Aphidicolin (Aph). Representative images are on the top, n=250; The p-values are indicated as follows: * <0.03, ** <0.0021, *** <0.0002, **** <0.0001. (D) Measurement of DNA single strand breaks by alkaline Comet assay in Pol eta −/− , PIP*, UBZ*, F1* and Pol eta +/+ cells treated with Aphidicolin (APH). The p-values are indicated as follows: * <0.03, ** <0.0021, *** <0.0002, **** <0.0001. Lengths were measured using the Adobe Photoshop Tools. (E) Time course experiment to measure recovery of cells after release into drug free media over the course of 48hours. Cells were collected at six time points (0, 4, 8, 12, 24, 28 hours) and expression levels of phospho-Histone H2AX <t>Ser139</t> were measured by Immunofluorescence staining. The p-values are indicated as follows: * <0.03, ** <0.0021, *** <0.0002, **** <0.0001. Scale bar 10 μm.
Phosphorylated γh2ax, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phosphorylated γh2ax/product/Novus Biologicals
Average 94 stars, based on 1 article reviews
phosphorylated γh2ax - by Bioz Stars, 2026-05
94/100 stars
  Buy from Supplier

Image Search Results


(A) Measuring expression levels of phospho-ATR protein, phospho-Chk1 Ser317 to assess replication checkpoint activation using western blotting. Expression levels of H3 were used as a loading control and (B) DNA fiber analysis of 50uM hydroxyurea (HU) treated to assess replication fork stalling in pol eta −/− , PIP*, UBZ*, F1* and pol eta+ /+ cells. The fork rate (CldU/IdU ratio) is indicated, n=150. The p-values are indicated as follows: * <0.03, ** <0.0021, *** <0.0002, **** <0.0001. Scale bar 10 μm; (C) Analysis of number of gH2AX foci (red) in the nucleus per cell in Pol eta −/− , PIP*, UBZ*, F1* and Pol eta +/+ cells when exposed to Aphidicolin (Aph). Representative images are on the top, n=250; The p-values are indicated as follows: * <0.03, ** <0.0021, *** <0.0002, **** <0.0001. (D) Measurement of DNA single strand breaks by alkaline Comet assay in Pol eta −/− , PIP*, UBZ*, F1* and Pol eta +/+ cells treated with Aphidicolin (APH). The p-values are indicated as follows: * <0.03, ** <0.0021, *** <0.0002, **** <0.0001. Lengths were measured using the Adobe Photoshop Tools. (E) Time course experiment to measure recovery of cells after release into drug free media over the course of 48hours. Cells were collected at six time points (0, 4, 8, 12, 24, 28 hours) and expression levels of phospho-Histone H2AX Ser139 were measured by Immunofluorescence staining. The p-values are indicated as follows: * <0.03, ** <0.0021, *** <0.0002, **** <0.0001. Scale bar 10 μm.

Journal: bioRxiv

Article Title: Polymerase Eta Recruits FANCD2 to Common Fragile Sites to Maintain Genome Stability

doi: 10.1101/2025.01.06.631600

Figure Lengend Snippet: (A) Measuring expression levels of phospho-ATR protein, phospho-Chk1 Ser317 to assess replication checkpoint activation using western blotting. Expression levels of H3 were used as a loading control and (B) DNA fiber analysis of 50uM hydroxyurea (HU) treated to assess replication fork stalling in pol eta −/− , PIP*, UBZ*, F1* and pol eta+ /+ cells. The fork rate (CldU/IdU ratio) is indicated, n=150. The p-values are indicated as follows: * <0.03, ** <0.0021, *** <0.0002, **** <0.0001. Scale bar 10 μm; (C) Analysis of number of gH2AX foci (red) in the nucleus per cell in Pol eta −/− , PIP*, UBZ*, F1* and Pol eta +/+ cells when exposed to Aphidicolin (Aph). Representative images are on the top, n=250; The p-values are indicated as follows: * <0.03, ** <0.0021, *** <0.0002, **** <0.0001. (D) Measurement of DNA single strand breaks by alkaline Comet assay in Pol eta −/− , PIP*, UBZ*, F1* and Pol eta +/+ cells treated with Aphidicolin (APH). The p-values are indicated as follows: * <0.03, ** <0.0021, *** <0.0002, **** <0.0001. Lengths were measured using the Adobe Photoshop Tools. (E) Time course experiment to measure recovery of cells after release into drug free media over the course of 48hours. Cells were collected at six time points (0, 4, 8, 12, 24, 28 hours) and expression levels of phospho-Histone H2AX Ser139 were measured by Immunofluorescence staining. The p-values are indicated as follows: * <0.03, ** <0.0021, *** <0.0002, **** <0.0001. Scale bar 10 μm.

Article Snippet: Primary Antibodies used-Primary antibodies used were anti-FANCD2 (1:1000, NB100-182, Novus), anti-cGAS (1:1000 D1D3G, Rabbit mAb), anti-Phospho-Histone H2A.X (Ser139), anti-XPV DNA Pol eta (Novus NBP1-87165, 1:2000), anti-BRCA1 (Sigma 07-434, 1:1000), anti-BRCA2 (Merck Millipore OP95, 1:1000) and anti-RAD51 (CosmobioUSA BAM-70-002-EX, 1:1000).

Techniques: Expressing, Activation Assay, Western Blot, Control, Alkaline Single Cell Gel Electrophoresis, Immunofluorescence, Staining