nb100-593 Search Results


rock2 antibody  (Bio-Techne corporation)
92
Bio-Techne corporation rock2 antibody
Rock2 Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rock2 antibody/product/Bio-Techne corporation
Average 92 stars, based on 1 article reviews
rock2 antibody - by Bioz Stars, 2026-05
92/100 stars
  Buy from Supplier
rock2  (Novus Biologicals)
91
Novus Biologicals rock2
FIGURE 3 KDM4A regulation of <t>ROCK2</t> and HUWE1 expression through histone demethylation. (A) Enrichment of KDM4A, H3K9me3, and H3K36me3 on ROCK2 and HUWE1 promoters assessed using ChIP. (B) Transcriptional levels of ROCK2 and HUWE1 evaluated by qRT-PCR. (C) Protein expressions of ROCK2 and HUWE1 analyzed by Western blot. Each experiment was independently repeated three times. Data in panels (A–C) were analyzed using two-way ANOVA, followed by Sidak's multiple comparisons test, *p < 0.05; **p < 0.01.
Rock2, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rock2/product/Novus Biologicals
Average 91 stars, based on 1 article reviews
rock2 - by Bioz Stars, 2026-05
91/100 stars
  Buy from Supplier

Image Search Results


FIGURE 3 KDM4A regulation of ROCK2 and HUWE1 expression through histone demethylation. (A) Enrichment of KDM4A, H3K9me3, and H3K36me3 on ROCK2 and HUWE1 promoters assessed using ChIP. (B) Transcriptional levels of ROCK2 and HUWE1 evaluated by qRT-PCR. (C) Protein expressions of ROCK2 and HUWE1 analyzed by Western blot. Each experiment was independently repeated three times. Data in panels (A–C) were analyzed using two-way ANOVA, followed by Sidak's multiple comparisons test, *p < 0.05; **p < 0.01.

Journal: The Kaohsiung journal of medical sciences

Article Title: The role of KDM4A-mediated histone methylation on temozolomide resistance in glioma cells through the HUWE1/ROCK2 axis.

doi: 10.1002/kjm2.12768

Figure Lengend Snippet: FIGURE 3 KDM4A regulation of ROCK2 and HUWE1 expression through histone demethylation. (A) Enrichment of KDM4A, H3K9me3, and H3K36me3 on ROCK2 and HUWE1 promoters assessed using ChIP. (B) Transcriptional levels of ROCK2 and HUWE1 evaluated by qRT-PCR. (C) Protein expressions of ROCK2 and HUWE1 analyzed by Western blot. Each experiment was independently repeated three times. Data in panels (A–C) were analyzed using two-way ANOVA, followed by Sidak's multiple comparisons test, *p < 0.05; **p < 0.01.

Article Snippet: The antibodies used included ROCK2 (1:1000, NB100-593, NOVUS) and HUWE1 (1:1000, ab70161, Abcam).

Techniques: Expressing, Quantitative RT-PCR, Western Blot

FIGURE 4 HUWE1 binding to ROCK2 and promotion of its ubiquitination degradation. (A) Binding relationship between HUWE1 and ROCK2 assessed by co-immunoprecipitation, with IgG serving as the isotype control. (B) ROCK2 ubiquitination level in U251MG and T98G cells evaluated by co- immunoprecipitation and Western blot, with IgG serving as the isotype control. (C) ROCK2 ubiquitination level detected after adding MG132 (DMSO treatment as control) to different transfected cells, with IgG serving as the isotype control. Each experiment was independently repeated three times.

Journal: The Kaohsiung journal of medical sciences

Article Title: The role of KDM4A-mediated histone methylation on temozolomide resistance in glioma cells through the HUWE1/ROCK2 axis.

doi: 10.1002/kjm2.12768

Figure Lengend Snippet: FIGURE 4 HUWE1 binding to ROCK2 and promotion of its ubiquitination degradation. (A) Binding relationship between HUWE1 and ROCK2 assessed by co-immunoprecipitation, with IgG serving as the isotype control. (B) ROCK2 ubiquitination level in U251MG and T98G cells evaluated by co- immunoprecipitation and Western blot, with IgG serving as the isotype control. (C) ROCK2 ubiquitination level detected after adding MG132 (DMSO treatment as control) to different transfected cells, with IgG serving as the isotype control. Each experiment was independently repeated three times.

Article Snippet: The antibodies used included ROCK2 (1:1000, NB100-593, NOVUS) and HUWE1 (1:1000, ab70161, Abcam).

Techniques: Binding Assay, Ubiquitin Proteomics, Immunoprecipitation, Control, Western Blot, Transfection

FIGURE 7 Chinese glioma genome atlas database analysis of ROCK2 and HUWE1 in GBM. (A–D) ROCK2 and HUWE1 expressions in different WHO grades of GBM and recurrent or primary GBM. (E and F) Survival analysis of GBM patients with different ROCK2 and HUWE1 expressions.

Journal: The Kaohsiung journal of medical sciences

Article Title: The role of KDM4A-mediated histone methylation on temozolomide resistance in glioma cells through the HUWE1/ROCK2 axis.

doi: 10.1002/kjm2.12768

Figure Lengend Snippet: FIGURE 7 Chinese glioma genome atlas database analysis of ROCK2 and HUWE1 in GBM. (A–D) ROCK2 and HUWE1 expressions in different WHO grades of GBM and recurrent or primary GBM. (E and F) Survival analysis of GBM patients with different ROCK2 and HUWE1 expressions.

Article Snippet: The antibodies used included ROCK2 (1:1000, NB100-593, NOVUS) and HUWE1 (1:1000, ab70161, Abcam).

Techniques:

FIGURE 8 Mechanism of KDM4A in glioma cell resistance to TMZ. In glioma cells, KDM4A reduces H3K9me3 to promote ROCK2 expression and reduces H3K36me3 to inhibit HUWE1 expression through histone demethylation. This represses HUWE1 binding to ROCK2 and inhibits ubiquitin degradation of ROCK2, ultimately promoting glioma cell resistance to TMZ.

Journal: The Kaohsiung journal of medical sciences

Article Title: The role of KDM4A-mediated histone methylation on temozolomide resistance in glioma cells through the HUWE1/ROCK2 axis.

doi: 10.1002/kjm2.12768

Figure Lengend Snippet: FIGURE 8 Mechanism of KDM4A in glioma cell resistance to TMZ. In glioma cells, KDM4A reduces H3K9me3 to promote ROCK2 expression and reduces H3K36me3 to inhibit HUWE1 expression through histone demethylation. This represses HUWE1 binding to ROCK2 and inhibits ubiquitin degradation of ROCK2, ultimately promoting glioma cell resistance to TMZ.

Article Snippet: The antibodies used included ROCK2 (1:1000, NB100-593, NOVUS) and HUWE1 (1:1000, ab70161, Abcam).

Techniques: Expressing, Binding Assay, Ubiquitin Proteomics