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Image Search Results
Journal: PLoS ONE
Article Title: Mycobacterium tuberculosis Infection and Tissue Factor Expression in Macrophages
doi: 10.1371/journal.pone.0045700
Figure Lengend Snippet: ( A ) MDMs were treated with TLR2 agonist (Pam3CSK4, 1 µg/ml), TLR4 agonist (MPLA, 1 µg/ml) and TLR9 agonist (ODN2006, 10 µg/ml) for 9 h. As a control, MDMs were also treated with γ- Mtb H37Rv (10 µg/ml). Cell surface TF activity was determined in factor X activation assay.* indicates significant difference compared to untreated cells (P≤0.01); # denotes significant difference compared to γ- Mtb treated cells (P<0.01) ( B ) MDMs were preincubated with CD14, TLR2 and TLR4 blocking antibodies (each, 10 µg/ml) either alone or in combination and with isotype control IgG for 45 min at 37°C and then cells were stimulated with γ- Mtb for 9 h. At the end of the stimulation period cell surface TF activity was measured in factor X activation assay. # denotes significant inhibition compared to γ- Mtb or LPS alone treated cells (P<0.05); paired t-test. Data are mean ± SEM (n = 3–8).
Article Snippet: TLR2 and
Techniques: Control, Activity Assay, Activation Assay, Blocking Assay, Inhibition