nanolc-ms Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher nanolc ms ms
    Nanolc Ms Ms, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1214 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nanolc ms ms/product/Thermo Fisher
    Average 99 stars, based on 1214 article reviews
    Price from $9.99 to $1999.99
    nanolc ms ms - by Bioz Stars, 2020-12
    99/100 stars
      Buy from Supplier

    92
    Agilent technologies nanolc ms ms
    <t>HPLC</t> of proteins in PMNs secretions induced by CHR and CAT and implication for innate immunity. A, B) Secretion from PMNs (1.10 8 cells) was induced during 30 min stimulation by (A) 20 µM CHR or (B) 20 µM CAT. The secreted proteins > 3 kDa were purified by RP-HPLC on a Macherey Nagel reverse-phase C18 column (4×250 mm; particle size 5 µM and pore size 100 nm). Numbered peaks in the chromatograms indicate protein fractions subjected to proteomic analyses. (C) Proteomic identification by <t>NanoLC-MS/MS</t> analysis of protein fractions involved in innate immunity (protein identification probability > 93%). The numbered HPLC fractions correspond to the peaks of secreted protein in the chromatograms presented in A and B, respectively.
    Nanolc Ms Ms, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 319 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nanolc ms ms/product/Agilent technologies
    Average 92 stars, based on 319 article reviews
    Price from $9.99 to $1999.99
    nanolc ms ms - by Bioz Stars, 2020-12
    92/100 stars
      Buy from Supplier

    93
    Waters Corporation nanolc ms ms
    Deciphering N -glycan structures and positioning on <t>GAP50</t> protein. A , GAP50 glycopeptides were identified using <t>nanoLC-MS/MS.</t> The MS/MS spectrum from the parent ion m/z = 1150.48 corresponds to the chymotryptic peptide 227 SNVTSRAW 234 with the N -glycosylation Hex 6 (HexNAc) 2 in position Asn228. B , The MS/MS spectrum from parent ion at m/z = 1329.03 corresponds to the tryptic peptide 136 NYTSEALR 143 with the N -glycosylation Hex 8 (HexNAc) 2 in position Asn136. C , The MS/MS spectrum from parent ion at m/z = 1414.31 corresponds to the tryptic peptide 77 VAANEHISFIASPGSNFLGGVSSLNDTR 104 with the N -glycosylation Hex 6 (HexNAc) 2 in position Asn101. Symbols were as follows: p , peptide; ■, N-acetylglucosamine; ●, mannose; ●, hexose. (D-G) Chymotryptic digestion of GAP50-HAFLAG profile using nanoLC-MS. D , Extracted ion chromatograms ( m/z 1150.0, 1231.0 for peak 1; 1245.5, 1326.5, 1407.5 for peak 2; 1261.0, 1342.1, 1423.1, for peak 3; ± 0.2 Da) indicated the elution zone of glycopeptides assigned by MS/MS. E , Combined mass spectrum of the glycopeptides containing Asn228 eluting at 17 min (peak 1). F , Combined mass spectrum of the glycopeptides containing Asn136 eluting at 19 min (peak 2). G , Combined mass spectrum of the glycopeptides containing Asn101 eluting at 23 min (peak 3).
    Nanolc Ms Ms, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 93/100, based on 158 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nanolc ms ms/product/Waters Corporation
    Average 93 stars, based on 158 article reviews
    Price from $9.99 to $1999.99
    nanolc ms ms - by Bioz Stars, 2020-12
    93/100 stars
      Buy from Supplier

    99
    Thermo Fisher ultimate 3000 nanolc ms ms system
    Deciphering N -glycan structures and positioning on <t>GAP50</t> protein. A , GAP50 glycopeptides were identified using <t>nanoLC-MS/MS.</t> The MS/MS spectrum from the parent ion m/z = 1150.48 corresponds to the chymotryptic peptide 227 SNVTSRAW 234 with the N -glycosylation Hex 6 (HexNAc) 2 in position Asn228. B , The MS/MS spectrum from parent ion at m/z = 1329.03 corresponds to the tryptic peptide 136 NYTSEALR 143 with the N -glycosylation Hex 8 (HexNAc) 2 in position Asn136. C , The MS/MS spectrum from parent ion at m/z = 1414.31 corresponds to the tryptic peptide 77 VAANEHISFIASPGSNFLGGVSSLNDTR 104 with the N -glycosylation Hex 6 (HexNAc) 2 in position Asn101. Symbols were as follows: p , peptide; ■, N-acetylglucosamine; ●, mannose; ●, hexose. (D-G) Chymotryptic digestion of GAP50-HAFLAG profile using nanoLC-MS. D , Extracted ion chromatograms ( m/z 1150.0, 1231.0 for peak 1; 1245.5, 1326.5, 1407.5 for peak 2; 1261.0, 1342.1, 1423.1, for peak 3; ± 0.2 Da) indicated the elution zone of glycopeptides assigned by MS/MS. E , Combined mass spectrum of the glycopeptides containing Asn228 eluting at 17 min (peak 1). F , Combined mass spectrum of the glycopeptides containing Asn136 eluting at 19 min (peak 2). G , Combined mass spectrum of the glycopeptides containing Asn101 eluting at 23 min (peak 3).
    Ultimate 3000 Nanolc Ms Ms System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultimate 3000 nanolc ms ms system/product/Thermo Fisher
    Average 99 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    ultimate 3000 nanolc ms ms system - by Bioz Stars, 2020-12
    99/100 stars
      Buy from Supplier

    90
    Agilent technologies nanolc esi ms ms analysis
    Sample process flow chart. M. incognita proteins were collected from the aqueous medium (the secretome) and from extracts of worms. Proteins in the tomato root exudates (TRE) that diffused across a 3,500Da filter membrane were also collected. Proteins were identified by <t>nanoLC</t> <t>ESI</t> MS/MS. A, B, C and D are Protein databases used for protein identification, complementary information for each database are available on Table S3 .
    Nanolc Esi Ms Ms Analysis, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 116 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nanolc esi ms ms analysis/product/Agilent technologies
    Average 90 stars, based on 116 article reviews
    Price from $9.99 to $1999.99
    nanolc esi ms ms analysis - by Bioz Stars, 2020-12
    90/100 stars
      Buy from Supplier

    99
    Thermo Fisher nanolc ms ms system
    Sample process flow chart. M. incognita proteins were collected from the aqueous medium (the secretome) and from extracts of worms. Proteins in the tomato root exudates (TRE) that diffused across a 3,500Da filter membrane were also collected. Proteins were identified by <t>nanoLC</t> <t>ESI</t> MS/MS. A, B, C and D are Protein databases used for protein identification, complementary information for each database are available on Table S3 .
    Nanolc Ms Ms System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 121 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nanolc ms ms system/product/Thermo Fisher
    Average 99 stars, based on 121 article reviews
    Price from $9.99 to $1999.99
    nanolc ms ms system - by Bioz Stars, 2020-12
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher online nanolc ms ms
    Sample process flow chart. M. incognita proteins were collected from the aqueous medium (the secretome) and from extracts of worms. Proteins in the tomato root exudates (TRE) that diffused across a 3,500Da filter membrane were also collected. Proteins were identified by <t>nanoLC</t> <t>ESI</t> MS/MS. A, B, C and D are Protein databases used for protein identification, complementary information for each database are available on Table S3 .
    Online Nanolc Ms Ms, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/online nanolc ms ms/product/Thermo Fisher
    Average 99 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    online nanolc ms ms - by Bioz Stars, 2020-12
    99/100 stars
      Buy from Supplier

    89
    Prottech Inc nanolc esi ms ms analysis
    MYL6B interacts with both MDM2 and p53. ( a , b ) Whole cell lysate from Huh7 cell transfected with myc3-vector or myc3-MDM2 were subjected to Co-IP with anti-myc agarose and followed by <t>NanoLC-ESI-MS/MS</t> analysis ( a ) or immunoblotting analysis with antibody against MDM2 or MYL6B (B). ( c ) Whole cell lysate from Huh7 cell transfected with 3FLAG-vector or 3FLAG-MYL6B were subjected to Co-IP with anti-FLAG Agarose Affinity Gel and followed by immunoblotting with antibody against MYL6B, MDM2 or p53. ( d ) Huh7 cell lysate was subjected to Co-IP with anti-MDM2 or IgG and followed by immunoblotting with antibody against MYL6B. ( e ) Whole cell lysate from Huh7 cell lysate was subjected to Co-IP with anti-p53 or IgG and followed by immunoblotting with antibody against MYL6B. ( f ) Increasing amounts (0, 1, and 2 μg) of MYL6B plasmid were transfected into Huh7 together with myc3-MDM2 and FLAG-p53. Anti-myc was used to immunoprecipitate myc3-MDM2 and immunoblotting were performed with p53, MYL6B and MDM2 antibodies
    Nanolc Esi Ms Ms Analysis, supplied by Prottech Inc, used in various techniques. Bioz Stars score: 89/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nanolc esi ms ms analysis/product/Prottech Inc
    Average 89 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    nanolc esi ms ms analysis - by Bioz Stars, 2020-12
    89/100 stars
      Buy from Supplier

    92
    Applied Biomics nanolc ms ms
    MYL6B interacts with both MDM2 and p53. ( a , b ) Whole cell lysate from Huh7 cell transfected with myc3-vector or myc3-MDM2 were subjected to Co-IP with anti-myc agarose and followed by <t>NanoLC-ESI-MS/MS</t> analysis ( a ) or immunoblotting analysis with antibody against MDM2 or MYL6B (B). ( c ) Whole cell lysate from Huh7 cell transfected with 3FLAG-vector or 3FLAG-MYL6B were subjected to Co-IP with anti-FLAG Agarose Affinity Gel and followed by immunoblotting with antibody against MYL6B, MDM2 or p53. ( d ) Huh7 cell lysate was subjected to Co-IP with anti-MDM2 or IgG and followed by immunoblotting with antibody against MYL6B. ( e ) Whole cell lysate from Huh7 cell lysate was subjected to Co-IP with anti-p53 or IgG and followed by immunoblotting with antibody against MYL6B. ( f ) Increasing amounts (0, 1, and 2 μg) of MYL6B plasmid were transfected into Huh7 together with myc3-MDM2 and FLAG-p53. Anti-myc was used to immunoprecipitate myc3-MDM2 and immunoblotting were performed with p53, MYL6B and MDM2 antibodies
    Nanolc Ms Ms, supplied by Applied Biomics, used in various techniques. Bioz Stars score: 92/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nanolc ms ms/product/Applied Biomics
    Average 92 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    nanolc ms ms - by Bioz Stars, 2020-12
    92/100 stars
      Buy from Supplier

    85
    Agilent technologies nanolc chip ms
    MYL6B interacts with both MDM2 and p53. ( a , b ) Whole cell lysate from Huh7 cell transfected with myc3-vector or myc3-MDM2 were subjected to Co-IP with anti-myc agarose and followed by <t>NanoLC-ESI-MS/MS</t> analysis ( a ) or immunoblotting analysis with antibody against MDM2 or MYL6B (B). ( c ) Whole cell lysate from Huh7 cell transfected with 3FLAG-vector or 3FLAG-MYL6B were subjected to Co-IP with anti-FLAG Agarose Affinity Gel and followed by immunoblotting with antibody against MYL6B, MDM2 or p53. ( d ) Huh7 cell lysate was subjected to Co-IP with anti-MDM2 or IgG and followed by immunoblotting with antibody against MYL6B. ( e ) Whole cell lysate from Huh7 cell lysate was subjected to Co-IP with anti-p53 or IgG and followed by immunoblotting with antibody against MYL6B. ( f ) Increasing amounts (0, 1, and 2 μg) of MYL6B plasmid were transfected into Huh7 together with myc3-MDM2 and FLAG-p53. Anti-myc was used to immunoprecipitate myc3-MDM2 and immunoblotting were performed with p53, MYL6B and MDM2 antibodies
    Nanolc Chip Ms, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nanolc chip ms/product/Agilent technologies
    Average 85 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    nanolc chip ms - by Bioz Stars, 2020-12
    85/100 stars
      Buy from Supplier

    85
    KYA Technologies Corporation nanolc ms ms systems
    MYL6B interacts with both MDM2 and p53. ( a , b ) Whole cell lysate from Huh7 cell transfected with myc3-vector or myc3-MDM2 were subjected to Co-IP with anti-myc agarose and followed by <t>NanoLC-ESI-MS/MS</t> analysis ( a ) or immunoblotting analysis with antibody against MDM2 or MYL6B (B). ( c ) Whole cell lysate from Huh7 cell transfected with 3FLAG-vector or 3FLAG-MYL6B were subjected to Co-IP with anti-FLAG Agarose Affinity Gel and followed by immunoblotting with antibody against MYL6B, MDM2 or p53. ( d ) Huh7 cell lysate was subjected to Co-IP with anti-MDM2 or IgG and followed by immunoblotting with antibody against MYL6B. ( e ) Whole cell lysate from Huh7 cell lysate was subjected to Co-IP with anti-p53 or IgG and followed by immunoblotting with antibody against MYL6B. ( f ) Increasing amounts (0, 1, and 2 μg) of MYL6B plasmid were transfected into Huh7 together with myc3-MDM2 and FLAG-p53. Anti-myc was used to immunoprecipitate myc3-MDM2 and immunoblotting were performed with p53, MYL6B and MDM2 antibodies
    Nanolc Ms Ms Systems, supplied by KYA Technologies Corporation, used in various techniques. Bioz Stars score: 85/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nanolc ms ms systems/product/KYA Technologies Corporation
    Average 85 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    nanolc ms ms systems - by Bioz Stars, 2020-12
    85/100 stars
      Buy from Supplier

    91
    Shimadzu Corporation nanolc ms ms
    MYL6B interacts with both MDM2 and p53. ( a , b ) Whole cell lysate from Huh7 cell transfected with myc3-vector or myc3-MDM2 were subjected to Co-IP with anti-myc agarose and followed by <t>NanoLC-ESI-MS/MS</t> analysis ( a ) or immunoblotting analysis with antibody against MDM2 or MYL6B (B). ( c ) Whole cell lysate from Huh7 cell transfected with 3FLAG-vector or 3FLAG-MYL6B were subjected to Co-IP with anti-FLAG Agarose Affinity Gel and followed by immunoblotting with antibody against MYL6B, MDM2 or p53. ( d ) Huh7 cell lysate was subjected to Co-IP with anti-MDM2 or IgG and followed by immunoblotting with antibody against MYL6B. ( e ) Whole cell lysate from Huh7 cell lysate was subjected to Co-IP with anti-p53 or IgG and followed by immunoblotting with antibody against MYL6B. ( f ) Increasing amounts (0, 1, and 2 μg) of MYL6B plasmid were transfected into Huh7 together with myc3-MDM2 and FLAG-p53. Anti-myc was used to immunoprecipitate myc3-MDM2 and immunoblotting were performed with p53, MYL6B and MDM2 antibodies
    Nanolc Ms Ms, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nanolc ms ms/product/Shimadzu Corporation
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nanolc ms ms - by Bioz Stars, 2020-12
    91/100 stars
      Buy from Supplier

    92
    Thermo Fisher 3000rslc nanolc ms ms system
    MYL6B interacts with both MDM2 and p53. ( a , b ) Whole cell lysate from Huh7 cell transfected with myc3-vector or myc3-MDM2 were subjected to Co-IP with anti-myc agarose and followed by <t>NanoLC-ESI-MS/MS</t> analysis ( a ) or immunoblotting analysis with antibody against MDM2 or MYL6B (B). ( c ) Whole cell lysate from Huh7 cell transfected with 3FLAG-vector or 3FLAG-MYL6B were subjected to Co-IP with anti-FLAG Agarose Affinity Gel and followed by immunoblotting with antibody against MYL6B, MDM2 or p53. ( d ) Huh7 cell lysate was subjected to Co-IP with anti-MDM2 or IgG and followed by immunoblotting with antibody against MYL6B. ( e ) Whole cell lysate from Huh7 cell lysate was subjected to Co-IP with anti-p53 or IgG and followed by immunoblotting with antibody against MYL6B. ( f ) Increasing amounts (0, 1, and 2 μg) of MYL6B plasmid were transfected into Huh7 together with myc3-MDM2 and FLAG-p53. Anti-myc was used to immunoprecipitate myc3-MDM2 and immunoblotting were performed with p53, MYL6B and MDM2 antibodies
    3000rslc Nanolc Ms Ms System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3000rslc nanolc ms ms system/product/Thermo Fisher
    Average 92 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    3000rslc nanolc ms ms system - by Bioz Stars, 2020-12
    92/100 stars
      Buy from Supplier

    93
    Agilent technologies agilent nanolc esi qtof ms system
    MYL6B interacts with both MDM2 and p53. ( a , b ) Whole cell lysate from Huh7 cell transfected with myc3-vector or myc3-MDM2 were subjected to Co-IP with anti-myc agarose and followed by <t>NanoLC-ESI-MS/MS</t> analysis ( a ) or immunoblotting analysis with antibody against MDM2 or MYL6B (B). ( c ) Whole cell lysate from Huh7 cell transfected with 3FLAG-vector or 3FLAG-MYL6B were subjected to Co-IP with anti-FLAG Agarose Affinity Gel and followed by immunoblotting with antibody against MYL6B, MDM2 or p53. ( d ) Huh7 cell lysate was subjected to Co-IP with anti-MDM2 or IgG and followed by immunoblotting with antibody against MYL6B. ( e ) Whole cell lysate from Huh7 cell lysate was subjected to Co-IP with anti-p53 or IgG and followed by immunoblotting with antibody against MYL6B. ( f ) Increasing amounts (0, 1, and 2 μg) of MYL6B plasmid were transfected into Huh7 together with myc3-MDM2 and FLAG-p53. Anti-myc was used to immunoprecipitate myc3-MDM2 and immunoblotting were performed with p53, MYL6B and MDM2 antibodies
    Agilent Nanolc Esi Qtof Ms System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/agilent nanolc esi qtof ms system/product/Agilent technologies
    Average 93 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    agilent nanolc esi qtof ms system - by Bioz Stars, 2020-12
    93/100 stars
      Buy from Supplier

    93
    Thermo Fisher ab sciex nanolc ms ms system
    MYL6B interacts with both MDM2 and p53. ( a , b ) Whole cell lysate from Huh7 cell transfected with myc3-vector or myc3-MDM2 were subjected to Co-IP with anti-myc agarose and followed by <t>NanoLC-ESI-MS/MS</t> analysis ( a ) or immunoblotting analysis with antibody against MDM2 or MYL6B (B). ( c ) Whole cell lysate from Huh7 cell transfected with 3FLAG-vector or 3FLAG-MYL6B were subjected to Co-IP with anti-FLAG Agarose Affinity Gel and followed by immunoblotting with antibody against MYL6B, MDM2 or p53. ( d ) Huh7 cell lysate was subjected to Co-IP with anti-MDM2 or IgG and followed by immunoblotting with antibody against MYL6B. ( e ) Whole cell lysate from Huh7 cell lysate was subjected to Co-IP with anti-p53 or IgG and followed by immunoblotting with antibody against MYL6B. ( f ) Increasing amounts (0, 1, and 2 μg) of MYL6B plasmid were transfected into Huh7 together with myc3-MDM2 and FLAG-p53. Anti-myc was used to immunoprecipitate myc3-MDM2 and immunoblotting were performed with p53, MYL6B and MDM2 antibodies
    Ab Sciex Nanolc Ms Ms System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ab sciex nanolc ms ms system/product/Thermo Fisher
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    ab sciex nanolc ms ms system - by Bioz Stars, 2020-12
    93/100 stars
      Buy from Supplier

    92
    Waters Corporation mass spectrometry nanolc ms
    MYL6B interacts with both MDM2 and p53. ( a , b ) Whole cell lysate from Huh7 cell transfected with myc3-vector or myc3-MDM2 were subjected to Co-IP with anti-myc agarose and followed by <t>NanoLC-ESI-MS/MS</t> analysis ( a ) or immunoblotting analysis with antibody against MDM2 or MYL6B (B). ( c ) Whole cell lysate from Huh7 cell transfected with 3FLAG-vector or 3FLAG-MYL6B were subjected to Co-IP with anti-FLAG Agarose Affinity Gel and followed by immunoblotting with antibody against MYL6B, MDM2 or p53. ( d ) Huh7 cell lysate was subjected to Co-IP with anti-MDM2 or IgG and followed by immunoblotting with antibody against MYL6B. ( e ) Whole cell lysate from Huh7 cell lysate was subjected to Co-IP with anti-p53 or IgG and followed by immunoblotting with antibody against MYL6B. ( f ) Increasing amounts (0, 1, and 2 μg) of MYL6B plasmid were transfected into Huh7 together with myc3-MDM2 and FLAG-p53. Anti-myc was used to immunoprecipitate myc3-MDM2 and immunoblotting were performed with p53, MYL6B and MDM2 antibodies
    Mass Spectrometry Nanolc Ms, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mass spectrometry nanolc ms/product/Waters Corporation
    Average 92 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    mass spectrometry nanolc ms - by Bioz Stars, 2020-12
    92/100 stars
      Buy from Supplier

    88
    Nanoflex high resolution tandem mass spectrometry a nanolc 2d plus system
    MYL6B interacts with both MDM2 and p53. ( a , b ) Whole cell lysate from Huh7 cell transfected with myc3-vector or myc3-MDM2 were subjected to Co-IP with anti-myc agarose and followed by <t>NanoLC-ESI-MS/MS</t> analysis ( a ) or immunoblotting analysis with antibody against MDM2 or MYL6B (B). ( c ) Whole cell lysate from Huh7 cell transfected with 3FLAG-vector or 3FLAG-MYL6B were subjected to Co-IP with anti-FLAG Agarose Affinity Gel and followed by immunoblotting with antibody against MYL6B, MDM2 or p53. ( d ) Huh7 cell lysate was subjected to Co-IP with anti-MDM2 or IgG and followed by immunoblotting with antibody against MYL6B. ( e ) Whole cell lysate from Huh7 cell lysate was subjected to Co-IP with anti-p53 or IgG and followed by immunoblotting with antibody against MYL6B. ( f ) Increasing amounts (0, 1, and 2 μg) of MYL6B plasmid were transfected into Huh7 together with myc3-MDM2 and FLAG-p53. Anti-myc was used to immunoprecipitate myc3-MDM2 and immunoblotting were performed with p53, MYL6B and MDM2 antibodies
    High Resolution Tandem Mass Spectrometry A Nanolc 2d Plus System, supplied by Nanoflex, used in various techniques. Bioz Stars score: 88/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high resolution tandem mass spectrometry a nanolc 2d plus system/product/Nanoflex
    Average 88 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    high resolution tandem mass spectrometry a nanolc 2d plus system - by Bioz Stars, 2020-12
    88/100 stars
      Buy from Supplier

    Image Search Results


    HPLC of proteins in PMNs secretions induced by CHR and CAT and implication for innate immunity. A, B) Secretion from PMNs (1.10 8 cells) was induced during 30 min stimulation by (A) 20 µM CHR or (B) 20 µM CAT. The secreted proteins > 3 kDa were purified by RP-HPLC on a Macherey Nagel reverse-phase C18 column (4×250 mm; particle size 5 µM and pore size 100 nm). Numbered peaks in the chromatograms indicate protein fractions subjected to proteomic analyses. (C) Proteomic identification by NanoLC-MS/MS analysis of protein fractions involved in innate immunity (protein identification probability > 93%). The numbered HPLC fractions correspond to the peaks of secreted protein in the chromatograms presented in A and B, respectively.

    Journal: PLoS ONE

    Article Title: Two Chromogranin A-Derived Peptides Induce Calcium Entry in Human Neutrophils by Calmodulin-Regulated Calcium Independent Phospholipase A2

    doi: 10.1371/journal.pone.0004501

    Figure Lengend Snippet: HPLC of proteins in PMNs secretions induced by CHR and CAT and implication for innate immunity. A, B) Secretion from PMNs (1.10 8 cells) was induced during 30 min stimulation by (A) 20 µM CHR or (B) 20 µM CAT. The secreted proteins > 3 kDa were purified by RP-HPLC on a Macherey Nagel reverse-phase C18 column (4×250 mm; particle size 5 µM and pore size 100 nm). Numbered peaks in the chromatograms indicate protein fractions subjected to proteomic analyses. (C) Proteomic identification by NanoLC-MS/MS analysis of protein fractions involved in innate immunity (protein identification probability > 93%). The numbered HPLC fractions correspond to the peaks of secreted protein in the chromatograms presented in A and B, respectively.

    Article Snippet: The proteins were digested with 40 µL of 12.5 ng/µL of modified porcine trypsin (Promega, Madison, WI, USA) in 25 mM NH4 HCO3 at 37°C for 14 h. The generated peptides were analyzed directly by NanoLC-MS/MS on an Agilent 1100 Series HPLC-Chip/MS system (Agilent Technologies, Palo Alto, USA) coupled to an HCT Ultra ion trap (Bruker Daltonics, Bremen, Germany).

    Techniques: High Performance Liquid Chromatography, Purification, Mass Spectrometry

    Phosphorylation sites in hTTP co-expressed with CIN85. Panels A, B, and C are extracted ion chromatograms (EICs) for residues 66–82 (A), 83–103 (B), and 183–194 (C) generated from nanoLC-ESI-MS runs derived from tandem MS data of ions m/z 915.4, m/z 1083.5, and m/z 658.5, corresponding to the phosphorylated peptides 66–82, 83–103, and 183–194, respectively (Data/Fig. not shown). The normalized responses demonstrated in the EICs are an estimation of the abundance of the ion of interest. The currents from the non-phosphorylated peptides are represented as solid lines, and the ion currents attributed to the phosphorylated forms of the same peptides are shown as dashed lines. Duplicate technical replicates yielded similar results.

    Journal: PLoS ONE

    Article Title: Phosphorylation of Human Tristetraprolin in Response to Its Interaction with the Cbl Interacting Protein CIN85

    doi: 10.1371/journal.pone.0009588

    Figure Lengend Snippet: Phosphorylation sites in hTTP co-expressed with CIN85. Panels A, B, and C are extracted ion chromatograms (EICs) for residues 66–82 (A), 83–103 (B), and 183–194 (C) generated from nanoLC-ESI-MS runs derived from tandem MS data of ions m/z 915.4, m/z 1083.5, and m/z 658.5, corresponding to the phosphorylated peptides 66–82, 83–103, and 183–194, respectively (Data/Fig. not shown). The normalized responses demonstrated in the EICs are an estimation of the abundance of the ion of interest. The currents from the non-phosphorylated peptides are represented as solid lines, and the ion currents attributed to the phosphorylated forms of the same peptides are shown as dashed lines. Duplicate technical replicates yielded similar results.

    Article Snippet: NanoLC-ESI-MS and MS/MS analyses were performed on hTTP digests using an Agilent 1100 nanoLC system on-line with an Agilent 6340 ion trap mass spectrometer with the Chip Cube Interface.

    Techniques: Generated, Mass Spectrometry, Derivative Assay

    Deciphering N -glycan structures and positioning on GAP50 protein. A , GAP50 glycopeptides were identified using nanoLC-MS/MS. The MS/MS spectrum from the parent ion m/z = 1150.48 corresponds to the chymotryptic peptide 227 SNVTSRAW 234 with the N -glycosylation Hex 6 (HexNAc) 2 in position Asn228. B , The MS/MS spectrum from parent ion at m/z = 1329.03 corresponds to the tryptic peptide 136 NYTSEALR 143 with the N -glycosylation Hex 8 (HexNAc) 2 in position Asn136. C , The MS/MS spectrum from parent ion at m/z = 1414.31 corresponds to the tryptic peptide 77 VAANEHISFIASPGSNFLGGVSSLNDTR 104 with the N -glycosylation Hex 6 (HexNAc) 2 in position Asn101. Symbols were as follows: p , peptide; ■, N-acetylglucosamine; ●, mannose; ●, hexose. (D-G) Chymotryptic digestion of GAP50-HAFLAG profile using nanoLC-MS. D , Extracted ion chromatograms ( m/z 1150.0, 1231.0 for peak 1; 1245.5, 1326.5, 1407.5 for peak 2; 1261.0, 1342.1, 1423.1, for peak 3; ± 0.2 Da) indicated the elution zone of glycopeptides assigned by MS/MS. E , Combined mass spectrum of the glycopeptides containing Asn228 eluting at 17 min (peak 1). F , Combined mass spectrum of the glycopeptides containing Asn136 eluting at 19 min (peak 2). G , Combined mass spectrum of the glycopeptides containing Asn101 eluting at 23 min (peak 3).

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: Unusual N-glycan Structures Required for Trafficking Toxoplasma gondii GAP50 to the Inner Membrane Complex Regulate Host Cell Entry Through Parasite Motility *

    doi: 10.1074/mcp.M111.008953

    Figure Lengend Snippet: Deciphering N -glycan structures and positioning on GAP50 protein. A , GAP50 glycopeptides were identified using nanoLC-MS/MS. The MS/MS spectrum from the parent ion m/z = 1150.48 corresponds to the chymotryptic peptide 227 SNVTSRAW 234 with the N -glycosylation Hex 6 (HexNAc) 2 in position Asn228. B , The MS/MS spectrum from parent ion at m/z = 1329.03 corresponds to the tryptic peptide 136 NYTSEALR 143 with the N -glycosylation Hex 8 (HexNAc) 2 in position Asn136. C , The MS/MS spectrum from parent ion at m/z = 1414.31 corresponds to the tryptic peptide 77 VAANEHISFIASPGSNFLGGVSSLNDTR 104 with the N -glycosylation Hex 6 (HexNAc) 2 in position Asn101. Symbols were as follows: p , peptide; ■, N-acetylglucosamine; ●, mannose; ●, hexose. (D-G) Chymotryptic digestion of GAP50-HAFLAG profile using nanoLC-MS. D , Extracted ion chromatograms ( m/z 1150.0, 1231.0 for peak 1; 1245.5, 1326.5, 1407.5 for peak 2; 1261.0, 1342.1, 1423.1, for peak 3; ± 0.2 Da) indicated the elution zone of glycopeptides assigned by MS/MS. E , Combined mass spectrum of the glycopeptides containing Asn228 eluting at 17 min (peak 1). F , Combined mass spectrum of the glycopeptides containing Asn136 eluting at 19 min (peak 2). G , Combined mass spectrum of the glycopeptides containing Asn101 eluting at 23 min (peak 3).

    Article Snippet: Five microliters of chymotrypsin GAP50 digest were analyzed by nanoLC-MS using the nanoACQUITY Ultra-Performance-LC system (UPLC, Waters, Milford, MA) coupled to a SYNAPT High Definition Mass Spectrometry quadrupole time-of-flight tandem mass spectrometer (Waters, Milford, MA) equipped with a nano-electrospray source.

    Techniques: Mass Spectrometry

    Sample process flow chart. M. incognita proteins were collected from the aqueous medium (the secretome) and from extracts of worms. Proteins in the tomato root exudates (TRE) that diffused across a 3,500Da filter membrane were also collected. Proteins were identified by nanoLC ESI MS/MS. A, B, C and D are Protein databases used for protein identification, complementary information for each database are available on Table S3 .

    Journal: PLoS Pathogens

    Article Title: Direct Identification of the Meloidogyne incognita Secretome Reveals Proteins with Host Cell Reprogramming Potential

    doi: 10.1371/journal.ppat.1000192

    Figure Lengend Snippet: Sample process flow chart. M. incognita proteins were collected from the aqueous medium (the secretome) and from extracts of worms. Proteins in the tomato root exudates (TRE) that diffused across a 3,500Da filter membrane were also collected. Proteins were identified by nanoLC ESI MS/MS. A, B, C and D are Protein databases used for protein identification, complementary information for each database are available on Table S3 .

    Article Snippet: nanoLC ESI MS/MS analysis An Agilent 1100 HPLC system (Agilent Technologies, Wilmington, DE) delivered a flow rate of 300 nL min−1 to a 3-phase capillary chromatography column through a splitter.

    Techniques: Flow Cytometry, Mass Spectrometry

    MYL6B interacts with both MDM2 and p53. ( a , b ) Whole cell lysate from Huh7 cell transfected with myc3-vector or myc3-MDM2 were subjected to Co-IP with anti-myc agarose and followed by NanoLC-ESI-MS/MS analysis ( a ) or immunoblotting analysis with antibody against MDM2 or MYL6B (B). ( c ) Whole cell lysate from Huh7 cell transfected with 3FLAG-vector or 3FLAG-MYL6B were subjected to Co-IP with anti-FLAG Agarose Affinity Gel and followed by immunoblotting with antibody against MYL6B, MDM2 or p53. ( d ) Huh7 cell lysate was subjected to Co-IP with anti-MDM2 or IgG and followed by immunoblotting with antibody against MYL6B. ( e ) Whole cell lysate from Huh7 cell lysate was subjected to Co-IP with anti-p53 or IgG and followed by immunoblotting with antibody against MYL6B. ( f ) Increasing amounts (0, 1, and 2 μg) of MYL6B plasmid were transfected into Huh7 together with myc3-MDM2 and FLAG-p53. Anti-myc was used to immunoprecipitate myc3-MDM2 and immunoblotting were performed with p53, MYL6B and MDM2 antibodies

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: MYL6B, a myosin light chain, promotes MDM2-mediated p53 degradation and drives HCC development

    doi: 10.1186/s13046-018-0693-7

    Figure Lengend Snippet: MYL6B interacts with both MDM2 and p53. ( a , b ) Whole cell lysate from Huh7 cell transfected with myc3-vector or myc3-MDM2 were subjected to Co-IP with anti-myc agarose and followed by NanoLC-ESI-MS/MS analysis ( a ) or immunoblotting analysis with antibody against MDM2 or MYL6B (B). ( c ) Whole cell lysate from Huh7 cell transfected with 3FLAG-vector or 3FLAG-MYL6B were subjected to Co-IP with anti-FLAG Agarose Affinity Gel and followed by immunoblotting with antibody against MYL6B, MDM2 or p53. ( d ) Huh7 cell lysate was subjected to Co-IP with anti-MDM2 or IgG and followed by immunoblotting with antibody against MYL6B. ( e ) Whole cell lysate from Huh7 cell lysate was subjected to Co-IP with anti-p53 or IgG and followed by immunoblotting with antibody against MYL6B. ( f ) Increasing amounts (0, 1, and 2 μg) of MYL6B plasmid were transfected into Huh7 together with myc3-MDM2 and FLAG-p53. Anti-myc was used to immunoprecipitate myc3-MDM2 and immunoblotting were performed with p53, MYL6B and MDM2 antibodies

    Article Snippet: Elution was resolved on NuPAGE™ Bis-Tris Gel (Life Technologies), silver stained, and subjected to NanoLC-ESI-MS/MS analysis sequencing and data analysis (ProtTech Inc.).

    Techniques: Transfection, Plasmid Preparation, Co-Immunoprecipitation Assay, Mass Spectrometry