nano-lc-nano-electrospray ionization esi - Search Results


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  • 99
    Thermo Fisher ultimate 3000 hplc system
    Ultimate 3000 Hplc System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3582 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies nano liquid chromatography nano lc electrospray ionization esi
    Nano Liquid Chromatography Nano Lc Electrospray Ionization Esi, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Waters Corporation nano lc electrospray ionization esi ms ms analysis
    Two-dimensional gel electrophoresis on antifatigue mice. 2-DE was performed and gels stained with SYPRO Ruby staining reagent. Gel images were analyzed by SameSpots (Nonlinear) software, and the protein spots significantly altered were selected and then subjected to <t>LC-ESI-Q-TOF</t> MS/MS analysis.
    Nano Lc Electrospray Ionization Esi Ms Ms Analysis, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher nano lc electrospray ionization source
    Two-dimensional gel electrophoresis on antifatigue mice. 2-DE was performed and gels stained with SYPRO Ruby staining reagent. Gel images were analyzed by SameSpots (Nonlinear) software, and the protein spots significantly altered were selected and then subjected to <t>LC-ESI-Q-TOF</t> MS/MS analysis.
    Nano Lc Electrospray Ionization Source, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 278 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    SCIEX nano lc electrospray ionization source
    Two-dimensional gel electrophoresis on antifatigue mice. 2-DE was performed and gels stained with SYPRO Ruby staining reagent. Gel images were analyzed by SameSpots (Nonlinear) software, and the protein spots significantly altered were selected and then subjected to <t>LC-ESI-Q-TOF</t> MS/MS analysis.
    Nano Lc Electrospray Ionization Source, supplied by SCIEX, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher nano liquid chromatography nano lc electrospray ionization source
    Two-dimensional gel electrophoresis on antifatigue mice. 2-DE was performed and gels stained with SYPRO Ruby staining reagent. Gel images were analyzed by SameSpots (Nonlinear) software, and the protein spots significantly altered were selected and then subjected to <t>LC-ESI-Q-TOF</t> MS/MS analysis.
    Nano Liquid Chromatography Nano Lc Electrospray Ionization Source, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Waters Corporation nano lc esi ms ms
    Two-dimensional gel electrophoresis on antifatigue mice. 2-DE was performed and gels stained with SYPRO Ruby staining reagent. Gel images were analyzed by SameSpots (Nonlinear) software, and the protein spots significantly altered were selected and then subjected to <t>LC-ESI-Q-TOF</t> MS/MS analysis.
    Nano Lc Esi Ms Ms, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 93/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bruker Corporation nano lc electrospray ionization ms ms
    Two-dimensional gel electrophoresis on antifatigue mice. 2-DE was performed and gels stained with SYPRO Ruby staining reagent. Gel images were analyzed by SameSpots (Nonlinear) software, and the protein spots significantly altered were selected and then subjected to <t>LC-ESI-Q-TOF</t> MS/MS analysis.
    Nano Lc Electrospray Ionization Ms Ms, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 86/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Waters Corporation nano electrospray ionization esi liquid chromatography mass spectrometry
    Two-dimensional gel electrophoresis on antifatigue mice. 2-DE was performed and gels stained with SYPRO Ruby staining reagent. Gel images were analyzed by SameSpots (Nonlinear) software, and the protein spots significantly altered were selected and then subjected to <t>LC-ESI-Q-TOF</t> MS/MS analysis.
    Nano Electrospray Ionization Esi Liquid Chromatography Mass Spectrometry, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 86/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies 1260 hplc chip nano electrospray ionization 6520 q tof tandem
    Two-dimensional gel electrophoresis on antifatigue mice. 2-DE was performed and gels stained with SYPRO Ruby staining reagent. Gel images were analyzed by SameSpots (Nonlinear) software, and the protein spots significantly altered were selected and then subjected to <t>LC-ESI-Q-TOF</t> MS/MS analysis.
    1260 Hplc Chip Nano Electrospray Ionization 6520 Q Tof Tandem, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 88/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies 1260 hplc chip nano electrospray ionization 6520 q tof ms system
    Two-dimensional gel electrophoresis on antifatigue mice. 2-DE was performed and gels stained with SYPRO Ruby staining reagent. Gel images were analyzed by SameSpots (Nonlinear) software, and the protein spots significantly altered were selected and then subjected to <t>LC-ESI-Q-TOF</t> MS/MS analysis.
    1260 Hplc Chip Nano Electrospray Ionization 6520 Q Tof Ms System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Waters Corporation nano liquid chromatography electrospray ionization mass spectroscopy
    Two-dimensional gel electrophoresis on antifatigue mice. 2-DE was performed and gels stained with SYPRO Ruby staining reagent. Gel images were analyzed by SameSpots (Nonlinear) software, and the protein spots significantly altered were selected and then subjected to <t>LC-ESI-Q-TOF</t> MS/MS analysis.
    Nano Liquid Chromatography Electrospray Ionization Mass Spectroscopy, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies 6210 electrospray ionization time of flight esi tof
    Two-dimensional gel electrophoresis on antifatigue mice. 2-DE was performed and gels stained with SYPRO Ruby staining reagent. Gel images were analyzed by SameSpots (Nonlinear) software, and the protein spots significantly altered were selected and then subjected to <t>LC-ESI-Q-TOF</t> MS/MS analysis.
    6210 Electrospray Ionization Time Of Flight Esi Tof, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher nano flow liquid chromatography electrospray ionization tandem mass spectrometry
    Two-dimensional gel electrophoresis on antifatigue mice. 2-DE was performed and gels stained with SYPRO Ruby staining reagent. Gel images were analyzed by SameSpots (Nonlinear) software, and the protein spots significantly altered were selected and then subjected to <t>LC-ESI-Q-TOF</t> MS/MS analysis.
    Nano Flow Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Waters Corporation nano liquid chromatography electron spray ionization tandem mass spectrometry lc esi ms ms
    Two-dimensional gel electrophoresis on antifatigue mice. 2-DE was performed and gels stained with SYPRO Ruby staining reagent. Gel images were analyzed by SameSpots (Nonlinear) software, and the protein spots significantly altered were selected and then subjected to <t>LC-ESI-Q-TOF</t> MS/MS analysis.
    Nano Liquid Chromatography Electron Spray Ionization Tandem Mass Spectrometry Lc Esi Ms Ms, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher proxeon nano spray esi source
    Two-dimensional gel electrophoresis on antifatigue mice. 2-DE was performed and gels stained with SYPRO Ruby staining reagent. Gel images were analyzed by SameSpots (Nonlinear) software, and the protein spots significantly altered were selected and then subjected to <t>LC-ESI-Q-TOF</t> MS/MS analysis.
    Proxeon Nano Spray Esi Source, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 70 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bruker Corporation hct ultra esi trap
    Two-dimensional gel electrophoresis on antifatigue mice. 2-DE was performed and gels stained with SYPRO Ruby staining reagent. Gel images were analyzed by SameSpots (Nonlinear) software, and the protein spots significantly altered were selected and then subjected to <t>LC-ESI-Q-TOF</t> MS/MS analysis.
    Hct Ultra Esi Trap, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 86/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New Objective Inc adpc ims picofrit nano esi probe
    Two-dimensional gel electrophoresis on antifatigue mice. 2-DE was performed and gels stained with SYPRO Ruby staining reagent. Gel images were analyzed by SameSpots (Nonlinear) software, and the protein spots significantly altered were selected and then subjected to <t>LC-ESI-Q-TOF</t> MS/MS analysis.
    Adpc Ims Picofrit Nano Esi Probe, supplied by New Objective Inc, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Agilent technologies nano lc esi qtof ms system
    Two-dimensional gel electrophoresis on antifatigue mice. 2-DE was performed and gels stained with SYPRO Ruby staining reagent. Gel images were analyzed by SameSpots (Nonlinear) software, and the protein spots significantly altered were selected and then subjected to <t>LC-ESI-Q-TOF</t> MS/MS analysis.
    Nano Lc Esi Qtof Ms System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 95/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bruker Corporation nano lc esi q tof ms
    Two-dimensional gel electrophoresis on antifatigue mice. 2-DE was performed and gels stained with SYPRO Ruby staining reagent. Gel images were analyzed by SameSpots (Nonlinear) software, and the protein spots significantly altered were selected and then subjected to <t>LC-ESI-Q-TOF</t> MS/MS analysis.
    Nano Lc Esi Q Tof Ms, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 89/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher nano esi lc ms ms
    Expression of ENPP3 in the uterine fluid of receptive and non-receptive phase. Tukey plot for the expression of ENPP3 quantified by <t>nano-ESI-LC/MS</t> in uterine fluids from early secretory phase (LH + 2) and mid-secretory phase (LH + 8) of the same set of women. A significant upregulation of ENPP3 (p = 0.0032) was observed in P dominant, receptive phase (LH + 8) on comparison with non-receptive phase (LH + 2).
    Nano Esi Lc Ms Ms, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher positive mode electrospray ionization tandem mass spectrometry
    Expression of ENPP3 in the uterine fluid of receptive and non-receptive phase. Tukey plot for the expression of ENPP3 quantified by <t>nano-ESI-LC/MS</t> in uterine fluids from early secretory phase (LH + 2) and mid-secretory phase (LH + 8) of the same set of women. A significant upregulation of ENPP3 (p = 0.0032) was observed in P dominant, receptive phase (LH + 8) on comparison with non-receptive phase (LH + 2).
    Positive Mode Electrospray Ionization Tandem Mass Spectrometry, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher nano lc esi ms ms a nanolc system
    Expression of ENPP3 in the uterine fluid of receptive and non-receptive phase. Tukey plot for the expression of ENPP3 quantified by <t>nano-ESI-LC/MS</t> in uterine fluids from early secretory phase (LH + 2) and mid-secretory phase (LH + 8) of the same set of women. A significant upregulation of ENPP3 (p = 0.0032) was observed in P dominant, receptive phase (LH + 8) on comparison with non-receptive phase (LH + 2).
    Nano Lc Esi Ms Ms A Nanolc System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher nano lc esi msms
    Expression of ENPP3 in the uterine fluid of receptive and non-receptive phase. Tukey plot for the expression of ENPP3 quantified by <t>nano-ESI-LC/MS</t> in uterine fluids from early secretory phase (LH + 2) and mid-secretory phase (LH + 8) of the same set of women. A significant upregulation of ENPP3 (p = 0.0032) was observed in P dominant, receptive phase (LH + 8) on comparison with non-receptive phase (LH + 2).
    Nano Lc Esi Msms, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher q exactive plus nano lc esi ms
    Identification of MtCEP1 peptide species in MtCEP1ox root and vector control exudates. (A) The pre-propeptide structure of MtCEP1 showing the two 15 amino acid peptide domains. (B) Eight species of MtCEP1 domain 1 (D1) peptide and one species of the domain 2 (D2) peptide were identified with their respective PTMs. HyP: hydroxylated proline; TaP: tri-arabinosylated proline. (C) The relative concentration of the nine MtCEP1 peptide species found in MtCEP1ox exudate and the five species found in the vector control exudate. Serial dilutions of the synthetic peptide were performed to establish a standard calibration curve (20amol to 200 femtomol) from which the concentration of each peptide was extrapolated. (D) The peptide species in MtCEP1ox samples were analysed using Quadrupole-Orbitrap and Q-TOF mass spectrometers. The ratio of the five peptide species identified correlated well between both <t>nano-LC-ESI-MS</t> systems. (E) The five most abundant peptides in MtCEP1ox sample eluted from the column based on their hydrophobicity as indicated by their retention time in the Quadrapole-Orbitrap. (F) The peptides in the vector control samples were detected in relative minute amounts as shown in the extracted ion chromatogram.
    Q Exactive Plus Nano Lc Esi Ms, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies nano lc esi ms
    Identification of MtCEP1 peptide species in MtCEP1ox root and vector control exudates. (A) The pre-propeptide structure of MtCEP1 showing the two 15 amino acid peptide domains. (B) Eight species of MtCEP1 domain 1 (D1) peptide and one species of the domain 2 (D2) peptide were identified with their respective PTMs. HyP: hydroxylated proline; TaP: tri-arabinosylated proline. (C) The relative concentration of the nine MtCEP1 peptide species found in MtCEP1ox exudate and the five species found in the vector control exudate. Serial dilutions of the synthetic peptide were performed to establish a standard calibration curve (20amol to 200 femtomol) from which the concentration of each peptide was extrapolated. (D) The peptide species in MtCEP1ox samples were analysed using Quadrupole-Orbitrap and Q-TOF mass spectrometers. The ratio of the five peptide species identified correlated well between both <t>nano-LC-ESI-MS</t> systems. (E) The five most abundant peptides in MtCEP1ox sample eluted from the column based on their hydrophobicity as indicated by their retention time in the Quadrapole-Orbitrap. (F) The peptides in the vector control samples were detected in relative minute amounts as shown in the extracted ion chromatogram.
    Nano Lc Esi Ms, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Waters Corporation lc esi ms ms analysis
    <t>Nano</t> <t>LC-ESI-MS/MS</t> spectrum scanning at m / z region 350 to 1600 Da from giant grouper roe protein band B with representative spectrum of identified tryptic peptides in doubly ( A ) and triply ( B ) charged signal.
    Lc Esi Ms Ms Analysis, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 95/100, based on 75 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher nano lc esi ltq orbitrap ms ms system
    <t>Nano</t> <t>LC-ESI-MS/MS</t> spectrum scanning at m / z region 350 to 1600 Da from giant grouper roe protein band B with representative spectrum of identified tryptic peptides in doubly ( A ) and triply ( B ) charged signal.
    Nano Lc Esi Ltq Orbitrap Ms Ms System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies 2d nano lc esi trap
    <t>Nano</t> <t>LC-ESI-MS/MS</t> spectrum scanning at m / z region 350 to 1600 Da from giant grouper roe protein band B with representative spectrum of identified tryptic peptides in doubly ( A ) and triply ( B ) charged signal.
    2d Nano Lc Esi Trap, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Waters Corporation nanouplc esi qtof ms system
    <t>Nano</t> <t>LC-ESI-MS/MS</t> spectrum scanning at m / z region 350 to 1600 Da from giant grouper roe protein band B with representative spectrum of identified tryptic peptides in doubly ( A ) and triply ( B ) charged signal.
    Nanouplc Esi Qtof Ms System, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies nano lc esi ms ms analysis
    <t>Nano</t> <t>LC-ESI-MS/MS</t> spectrum scanning at m / z region 350 to 1600 Da from giant grouper roe protein band B with representative spectrum of identified tryptic peptides in doubly ( A ) and triply ( B ) charged signal.
    Nano Lc Esi Ms Ms Analysis, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies nano lc esi ms ms system
    <t>Nano</t> <t>LC-ESI-MS/MS</t> spectrum scanning at m / z region 350 to 1600 Da from giant grouper roe protein band B with representative spectrum of identified tryptic peptides in doubly ( A ) and triply ( B ) charged signal.
    Nano Lc Esi Ms Ms System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 86/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nano lc esi ms ms system/product/Agilent technologies
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    Two-dimensional gel electrophoresis on antifatigue mice. 2-DE was performed and gels stained with SYPRO Ruby staining reagent. Gel images were analyzed by SameSpots (Nonlinear) software, and the protein spots significantly altered were selected and then subjected to LC-ESI-Q-TOF MS/MS analysis.

    Journal: International Journal of Medical Sciences

    Article Title: Rutin, a Flavonoid and Principal Component of Saussurea Involucrata, Attenuates Physical Fatigue in a Forced Swimming Mouse Model

    doi: 10.7150/ijms.8220

    Figure Lengend Snippet: Two-dimensional gel electrophoresis on antifatigue mice. 2-DE was performed and gels stained with SYPRO Ruby staining reagent. Gel images were analyzed by SameSpots (Nonlinear) software, and the protein spots significantly altered were selected and then subjected to LC-ESI-Q-TOF MS/MS analysis.

    Article Snippet: LC-nano ESI-Q-TOF MS/MS analysis The resulting peptide mixture was subjected to the CapLC system (Waters, Milford, MA,) utilizing a capillary column (75 m i.d., 10 cm in length, C SUN, Taiwan) with a linear gradient from 5% to 50% acetonitrile containing 0.1% formic acid over 46 min.

    Techniques: Two-Dimensional Gel Electrophoresis, Electrophoresis, Mouse Assay, Staining, Software, Mass Spectrometry

    Expression of ENPP3 in the uterine fluid of receptive and non-receptive phase. Tukey plot for the expression of ENPP3 quantified by nano-ESI-LC/MS in uterine fluids from early secretory phase (LH + 2) and mid-secretory phase (LH + 8) of the same set of women. A significant upregulation of ENPP3 (p = 0.0032) was observed in P dominant, receptive phase (LH + 8) on comparison with non-receptive phase (LH + 2).

    Journal: Scientific Reports

    Article Title: Compartmentalized gene expression profiling of receptive endometrium reveals progesterone regulated ENPP3 is differentially expressed and secreted in glycosylated form

    doi: 10.1038/srep33811

    Figure Lengend Snippet: Expression of ENPP3 in the uterine fluid of receptive and non-receptive phase. Tukey plot for the expression of ENPP3 quantified by nano-ESI-LC/MS in uterine fluids from early secretory phase (LH + 2) and mid-secretory phase (LH + 8) of the same set of women. A significant upregulation of ENPP3 (p = 0.0032) was observed in P dominant, receptive phase (LH + 8) on comparison with non-receptive phase (LH + 2).

    Article Snippet: Proteins were then reduced, alkylated and in-gel digested with dimethylated porcine trypsin (Sigma) followed by analysis with nano-ESI-LC/MS/MS (Dionex Ultimate 3000 RSLC and Q Exactive MS/MS, Thermo Fisher Scientific at University of Tartu) using 2 h reversed phase gradients and a top-10 data-dependent acquisition method.

    Techniques: Expressing, Liquid Chromatography with Mass Spectroscopy

    Identification of MtCEP1 peptide species in MtCEP1ox root and vector control exudates. (A) The pre-propeptide structure of MtCEP1 showing the two 15 amino acid peptide domains. (B) Eight species of MtCEP1 domain 1 (D1) peptide and one species of the domain 2 (D2) peptide were identified with their respective PTMs. HyP: hydroxylated proline; TaP: tri-arabinosylated proline. (C) The relative concentration of the nine MtCEP1 peptide species found in MtCEP1ox exudate and the five species found in the vector control exudate. Serial dilutions of the synthetic peptide were performed to establish a standard calibration curve (20amol to 200 femtomol) from which the concentration of each peptide was extrapolated. (D) The peptide species in MtCEP1ox samples were analysed using Quadrupole-Orbitrap and Q-TOF mass spectrometers. The ratio of the five peptide species identified correlated well between both nano-LC-ESI-MS systems. (E) The five most abundant peptides in MtCEP1ox sample eluted from the column based on their hydrophobicity as indicated by their retention time in the Quadrapole-Orbitrap. (F) The peptides in the vector control samples were detected in relative minute amounts as shown in the extracted ion chromatogram.

    Journal: Journal of Experimental Botany

    Article Title: Novel MtCEP1 peptides produced in vivo differentially regulate root development in Medicago truncatula

    doi: 10.1093/jxb/erv008

    Figure Lengend Snippet: Identification of MtCEP1 peptide species in MtCEP1ox root and vector control exudates. (A) The pre-propeptide structure of MtCEP1 showing the two 15 amino acid peptide domains. (B) Eight species of MtCEP1 domain 1 (D1) peptide and one species of the domain 2 (D2) peptide were identified with their respective PTMs. HyP: hydroxylated proline; TaP: tri-arabinosylated proline. (C) The relative concentration of the nine MtCEP1 peptide species found in MtCEP1ox exudate and the five species found in the vector control exudate. Serial dilutions of the synthetic peptide were performed to establish a standard calibration curve (20amol to 200 femtomol) from which the concentration of each peptide was extrapolated. (D) The peptide species in MtCEP1ox samples were analysed using Quadrupole-Orbitrap and Q-TOF mass spectrometers. The ratio of the five peptide species identified correlated well between both nano-LC-ESI-MS systems. (E) The five most abundant peptides in MtCEP1ox sample eluted from the column based on their hydrophobicity as indicated by their retention time in the Quadrapole-Orbitrap. (F) The peptides in the vector control samples were detected in relative minute amounts as shown in the extracted ion chromatogram.

    Article Snippet: The peptide wash was lyophilized overnight and resuspended in 400 µL of 3% acetonitrile with 0.1% formic acid prior to analysis using Q Exactive Plus nano-LC-ESI-MS/MS (Thermo Fisher Scientific, Waltham, MA, USA).

    Techniques: Plasmid Preparation, Concentration Assay, Mass Spectrometry

    Nano LC-ESI-MS/MS spectrum scanning at m / z region 350 to 1600 Da from giant grouper roe protein band B with representative spectrum of identified tryptic peptides in doubly ( A ) and triply ( B ) charged signal.

    Journal: Molecules

    Article Title: In Silico Analysis of Bioactive Peptides Released from Giant Grouper (Epinephelus lanceolatus) Roe Proteins Identified by Proteomics Approach

    doi: 10.3390/molecules23112910

    Figure Lengend Snippet: Nano LC-ESI-MS/MS spectrum scanning at m / z region 350 to 1600 Da from giant grouper roe protein band B with representative spectrum of identified tryptic peptides in doubly ( A ) and triply ( B ) charged signal.

    Article Snippet: Nano LC-ESI-MS/MS Analysis Tryptic peptide extracts were subjected to nano LC-ESI-MS/MS analysis using a nanoAcquity system (Waters, Milford, MA, USA).

    Techniques: Mass Spectrometry