n. meningitidis Search Results


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  • 99
    ATCC n meningitidis
    Colorimetric and fluorescence based detection for NAATs in 3D printed reactor array. A) Representative photographs of colorimetric LAMP assay for detection of N. <t>meningitidis</t> with 0, 50, 500 and 5000 CFU/reaction on the same chip, alongside LAMP fluorescence based image at given time interval. B) LAMP amplification curves for P. falciparum with 0, 0.1 1, 10, 100, 1000 pg per reaction. C) Calibration curve for P. falciparum as function of log target concentration, n=3. D) LAMP amplification curves for N. meningitidis with 0, 50, 500, 5000 CFU per reaction. E) Calibration curve for N. meningitidis as function of log target concentration, n=3. WarmStart ® LAMP master mix was used.
    N Meningitidis, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 99 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore tetracycline
    Colorimetric and fluorescence based detection for NAATs in 3D printed reactor array. A) Representative photographs of colorimetric LAMP assay for detection of N. <t>meningitidis</t> with 0, 50, 500 and 5000 CFU/reaction on the same chip, alongside LAMP fluorescence based image at given time interval. B) LAMP amplification curves for P. falciparum with 0, 0.1 1, 10, 100, 1000 pg per reaction. C) Calibration curve for P. falciparum as function of log target concentration, n=3. D) LAMP amplification curves for N. meningitidis with 0, 50, 500, 5000 CFU per reaction. E) Calibration curve for N. meningitidis as function of log target concentration, n=3. WarmStart ® LAMP master mix was used.
    Tetracycline, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 4615 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck & Co n meningitidis
    Colorimetric and fluorescence based detection for NAATs in 3D printed reactor array. A) Representative photographs of colorimetric LAMP assay for detection of N. <t>meningitidis</t> with 0, 50, 500 and 5000 CFU/reaction on the same chip, alongside LAMP fluorescence based image at given time interval. B) LAMP amplification curves for P. falciparum with 0, 0.1 1, 10, 100, 1000 pg per reaction. C) Calibration curve for P. falciparum as function of log target concentration, n=3. D) LAMP amplification curves for N. meningitidis with 0, 50, 500, 5000 CFU per reaction. E) Calibration curve for N. meningitidis as function of log target concentration, n=3. WarmStart ® LAMP master mix was used.
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    Connaught Labs n meningitidis
    Colorimetric and fluorescence based detection for NAATs in 3D printed reactor array. A) Representative photographs of colorimetric LAMP assay for detection of N. <t>meningitidis</t> with 0, 50, 500 and 5000 CFU/reaction on the same chip, alongside LAMP fluorescence based image at given time interval. B) LAMP amplification curves for P. falciparum with 0, 0.1 1, 10, 100, 1000 pg per reaction. C) Calibration curve for P. falciparum as function of log target concentration, n=3. D) LAMP amplification curves for N. meningitidis with 0, 50, 500, 5000 CFU per reaction. E) Calibration curve for N. meningitidis as function of log target concentration, n=3. WarmStart ® LAMP master mix was used.
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    Virostat c n meningitidis
    Colorimetric and fluorescence based detection for NAATs in 3D printed reactor array. A) Representative photographs of colorimetric LAMP assay for detection of N. <t>meningitidis</t> with 0, 50, 500 and 5000 CFU/reaction on the same chip, alongside LAMP fluorescence based image at given time interval. B) LAMP amplification curves for P. falciparum with 0, 0.1 1, 10, 100, 1000 pg per reaction. C) Calibration curve for P. falciparum as function of log target concentration, n=3. D) LAMP amplification curves for N. meningitidis with 0, 50, 500, 5000 CFU per reaction. E) Calibration curve for N. meningitidis as function of log target concentration, n=3. WarmStart ® LAMP master mix was used.
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    Clinical and Laboratory Standards Institute n meningitidis
    Colorimetric and fluorescence based detection for NAATs in 3D printed reactor array. A) Representative photographs of colorimetric LAMP assay for detection of N. <t>meningitidis</t> with 0, 50, 500 and 5000 CFU/reaction on the same chip, alongside LAMP fluorescence based image at given time interval. B) LAMP amplification curves for P. falciparum with 0, 0.1 1, 10, 100, 1000 pg per reaction. C) Calibration curve for P. falciparum as function of log target concentration, n=3. D) LAMP amplification curves for N. meningitidis with 0, 50, 500, 5000 CFU per reaction. E) Calibration curve for N. meningitidis as function of log target concentration, n=3. WarmStart ® LAMP master mix was used.
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    Becton Dickinson n meningitidis
    Lack of cross-complementation of E. coli and N. <t>meningitidis</t> Skp. (A) The indicated N. meningitidis strains were grown in the presence or absence of 1 mM IPTG as specified below the gel and analyzed as cell lysates by denaturing SDS-PAGE and Coomassie
    N Meningitidis, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Copan Diagnostics n meningitidis
    Lack of cross-complementation of E. coli and N. <t>meningitidis</t> Skp. (A) The indicated N. meningitidis strains were grown in the presence or absence of 1 mM IPTG as specified below the gel and analyzed as cell lysates by denaturing SDS-PAGE and Coomassie
    N Meningitidis, supplied by Copan Diagnostics, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Starplex Scientific n meningitidis
    Lack of cross-complementation of E. coli and N. <t>meningitidis</t> Skp. (A) The indicated N. meningitidis strains were grown in the presence or absence of 1 mM IPTG as specified below the gel and analyzed as cell lysates by denaturing SDS-PAGE and Coomassie
    N Meningitidis, supplied by Starplex Scientific, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Addgene inc n meningitidis
    Lack of cross-complementation of E. coli and N. <t>meningitidis</t> Skp. (A) The indicated N. meningitidis strains were grown in the presence or absence of 1 mM IPTG as specified below the gel and analyzed as cell lysates by denaturing SDS-PAGE and Coomassie
    N Meningitidis, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Mimotopes n meningitidis
    Lack of cross-complementation of E. coli and N. <t>meningitidis</t> Skp. (A) The indicated N. meningitidis strains were grown in the presence or absence of 1 mM IPTG as specified below the gel and analyzed as cell lysates by denaturing SDS-PAGE and Coomassie
    N Meningitidis, supplied by Mimotopes, used in various techniques. Bioz Stars score: 84/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher n meningitidis
    Lack of cross-complementation of E. coli and N. <t>meningitidis</t> Skp. (A) The indicated N. meningitidis strains were grown in the presence or absence of 1 mM IPTG as specified below the gel and analyzed as cell lysates by denaturing SDS-PAGE and Coomassie
    N Meningitidis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology n meningitidis pib
    Lack of cross-complementation of E. coli and N. <t>meningitidis</t> Skp. (A) The indicated N. meningitidis strains were grown in the presence or absence of 1 mM IPTG as specified below the gel and analyzed as cell lysates by denaturing SDS-PAGE and Coomassie
    N Meningitidis Pib, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 87/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Connaught Labs n meningitidis quadrivalent
    Lack of cross-complementation of E. coli and N. <t>meningitidis</t> Skp. (A) The indicated N. meningitidis strains were grown in the presence or absence of 1 mM IPTG as specified below the gel and analyzed as cell lysates by denaturing SDS-PAGE and Coomassie
    N Meningitidis Quadrivalent, supplied by Connaught Labs, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Abcam wc n meningitidis
    Immunofluorescence microscopy of WCs of strains MC58, MC58Lnt and MC58LntC. To confirm the presence of meningococcal cells, cells were incubated with FITC‐labelled rabbit polyclonal IgG raised against WC N. <t>meningitidis</t> (left and right panel). To compare FHbp cell surface expression between the strains, cells were also incubated with anti‐FHbp antibody JAR4 that was detected by Alexa Fluor 555 labelled donkey anti‐mouse IgG secondary antibody (right panel).
    Wc N Meningitidis, supplied by Abcam, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Difco n meningitidis antiserum
    Immunofluorescence microscopy of WCs of strains MC58, MC58Lnt and MC58LntC. To confirm the presence of meningococcal cells, cells were incubated with FITC‐labelled rabbit polyclonal IgG raised against WC N. <t>meningitidis</t> (left and right panel). To compare FHbp cell surface expression between the strains, cells were also incubated with anti‐FHbp antibody JAR4 that was detected by Alexa Fluor 555 labelled donkey anti‐mouse IgG secondary antibody (right panel).
    N Meningitidis Antiserum, supplied by Difco, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson unencapsulated n meningitidis
    Immunofluorescence microscopy of WCs of strains MC58, MC58Lnt and MC58LntC. To confirm the presence of meningococcal cells, cells were incubated with FITC‐labelled rabbit polyclonal IgG raised against WC N. <t>meningitidis</t> (left and right panel). To compare FHbp cell surface expression between the strains, cells were also incubated with anti‐FHbp antibody JAR4 that was detected by Alexa Fluor 555 labelled donkey anti‐mouse IgG secondary antibody (right panel).
    Unencapsulated N Meningitidis, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore n meningitidis group b
    Immunofluorescence microscopy of WCs of strains MC58, MC58Lnt and MC58LntC. To confirm the presence of meningococcal cells, cells were incubated with FITC‐labelled rabbit polyclonal IgG raised against WC N. <t>meningitidis</t> (left and right panel). To compare FHbp cell surface expression between the strains, cells were also incubated with anti‐FHbp antibody JAR4 that was detected by Alexa Fluor 555 labelled donkey anti‐mouse IgG secondary antibody (right panel).
    N Meningitidis Group B, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    US Biological Life Sciences rabbit antibody against n meningitidis
    Dynamic meningococcal disease progression selects for Opa expression. (A) Mice with mild-disease pattern were imaged over 4 days using a detection scale of 200/2000 counts. Bioluminescent signals from the mice were undetectable at 2 days (2d) post-infection. From day 3 (3d), the signals reappeared either in the brain (upper panel) or in the whole body (lower panel). Images are representative of each subgroup. Each panel follows one mouse over time. Bacterial loads in blood and CSF at indicated time points post-challenge are shown. (B) N. <t>meningitidis</t> was isolated from blood, CSF or nasal washes (N wash) at day 1, 3 and 4 after infection. Recovered isolates together with the initial inoculum, were analyzed for expression of Opa proteins (30 kDa), PilC (110 kDa), and pili (PilE subunit 16 kDa) by immunoblots. LOS (6.5 kDa) was analyzed by Tricine SDS-PAGE.
    Rabbit Antibody Against N Meningitidis, supplied by US Biological Life Sciences, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Thermo Fisher wellcogen n meningitidis acyw135 latex reagent
    Dynamic meningococcal disease progression selects for Opa expression. (A) Mice with mild-disease pattern were imaged over 4 days using a detection scale of 200/2000 counts. Bioluminescent signals from the mice were undetectable at 2 days (2d) post-infection. From day 3 (3d), the signals reappeared either in the brain (upper panel) or in the whole body (lower panel). Images are representative of each subgroup. Each panel follows one mouse over time. Bacterial loads in blood and CSF at indicated time points post-challenge are shown. (B) N. <t>meningitidis</t> was isolated from blood, CSF or nasal washes (N wash) at day 1, 3 and 4 after infection. Recovered isolates together with the initial inoculum, were analyzed for expression of Opa proteins (30 kDa), PilC (110 kDa), and pili (PilE subunit 16 kDa) by immunoblots. LOS (6.5 kDa) was analyzed by Tricine SDS-PAGE.
    Wellcogen N Meningitidis Acyw135 Latex Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Merck & Co n meningitidis strain c11
    Dynamic meningococcal disease progression selects for Opa expression. (A) Mice with mild-disease pattern were imaged over 4 days using a detection scale of 200/2000 counts. Bioluminescent signals from the mice were undetectable at 2 days (2d) post-infection. From day 3 (3d), the signals reappeared either in the brain (upper panel) or in the whole body (lower panel). Images are representative of each subgroup. Each panel follows one mouse over time. Bacterial loads in blood and CSF at indicated time points post-challenge are shown. (B) N. <t>meningitidis</t> was isolated from blood, CSF or nasal washes (N wash) at day 1, 3 and 4 after infection. Recovered isolates together with the initial inoculum, were analyzed for expression of Opa proteins (30 kDa), PilC (110 kDa), and pili (PilE subunit 16 kDa) by immunoblots. LOS (6.5 kDa) was analyzed by Tricine SDS-PAGE.
    N Meningitidis Strain C11, supplied by Merck & Co, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Pasteur Institute n meningitidis strain 8013
    Dynamic meningococcal disease progression selects for Opa expression. (A) Mice with mild-disease pattern were imaged over 4 days using a detection scale of 200/2000 counts. Bioluminescent signals from the mice were undetectable at 2 days (2d) post-infection. From day 3 (3d), the signals reappeared either in the brain (upper panel) or in the whole body (lower panel). Images are representative of each subgroup. Each panel follows one mouse over time. Bacterial loads in blood and CSF at indicated time points post-challenge are shown. (B) N. <t>meningitidis</t> was isolated from blood, CSF or nasal washes (N wash) at day 1, 3 and 4 after infection. Recovered isolates together with the initial inoculum, were analyzed for expression of Opa proteins (30 kDa), PilC (110 kDa), and pili (PilE subunit 16 kDa) by immunoblots. LOS (6.5 kDa) was analyzed by Tricine SDS-PAGE.
    N Meningitidis Strain 8013, supplied by Pasteur Institute, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    81
    Promega n meningitidis strain 608b
    Bactericidal activity of protein A–purified mAb Me-1 against N. <t>meningitidis</t> strain <t>608B.</t> Guinea pig serum ( closed circles ) or heat inactivated guinea pig serum ( open circles ) were used as the source of complement to evaluate the bactericidal activity.
    N Meningitidis Strain 608b, supplied by Promega, used in various techniques. Bioz Stars score: 81/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Trinity Biotech polyclonal antibody against n meningitidis
    Bactericidal activity of protein A–purified mAb Me-1 against N. <t>meningitidis</t> strain <t>608B.</t> Guinea pig serum ( closed circles ) or heat inactivated guinea pig serum ( open circles ) were used as the source of complement to evaluate the bactericidal activity.
    Polyclonal Antibody Against N Meningitidis, supplied by Trinity Biotech, used in various techniques. Bioz Stars score: 86/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Pasteur Institute pbad giiia strain n meningitidis csbpi
    Bactericidal activity of protein A–purified mAb Me-1 against N. <t>meningitidis</t> strain <t>608B.</t> Guinea pig serum ( closed circles ) or heat inactivated guinea pig serum ( open circles ) were used as the source of complement to evaluate the bactericidal activity.
    Pbad Giiia Strain N Meningitidis Csbpi, supplied by Pasteur Institute, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC n meningitidis atcc 13102
    Bactericidal activity of protein A–purified mAb Me-1 against N. <t>meningitidis</t> strain <t>608B.</t> Guinea pig serum ( closed circles ) or heat inactivated guinea pig serum ( open circles ) were used as the source of complement to evaluate the bactericidal activity.
    N Meningitidis Atcc 13102, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC n meningitidis serogroup a
    Bactericidal activity of protein A–purified mAb Me-1 against N. <t>meningitidis</t> strain <t>608B.</t> Guinea pig serum ( closed circles ) or heat inactivated guinea pig serum ( open circles ) were used as the source of complement to evaluate the bactericidal activity.
    N Meningitidis Serogroup A, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC serogroup c n meningitidis
    Bactericidal activity of protein A–purified mAb Me-1 against N. <t>meningitidis</t> strain <t>608B.</t> Guinea pig serum ( closed circles ) or heat inactivated guinea pig serum ( open circles ) were used as the source of complement to evaluate the bactericidal activity.
    Serogroup C N Meningitidis, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Thermo Fisher anti n meningitidis strain 2c4 3
    N. <t>meningitidis</t> do not cross the epithelial layer. (A) Z-stack from confocal 3D reconstruction of Calu-3 cell layer infected 24 h with 10 6 bacteria of the wild-type strain <t>(2C4.3</t> WT) or the strain defective for type IV pili (2C4.3 Δ pilE ). The Calu-3 cell layer was fixed and immunostained with anti-2C4.3 antibody (green). Cells were stained with Alexa Fluor-conjugated phalloidin (red), and nuclei were stained with DAPI (blue). Bar, 20 μm. (B) Transmission electron microscopy images (longitudinal sections) of Calu-3 cell layer infected for 24 h with 10 6 wild-type meningococci. (C) Kaplan-Meier plots showing the traversal of wild-type N. meningitidis across the Calu-3 cell layer. Data were expressed as percentages of invaded basal chamber. For untreated cells, eight filters were infected with 10 4 or 10 2 bacteria, and three independent experiments were performed. For IL-4- or IL-13-treated cells, four filters were infected with 10 4 or 10 2 bacteria, and two independent experiments were performed.
    Anti N Meningitidis Strain 2c4 3, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC n meningitidis serogroup w strain
    Transmission electron micrographs showing the presence of surface capsular polysaccharide for ( a ) Neisseria <t>meningitidis</t> <t>serogroup</t> W (ATCC 35559) and ( b ) absence of capsule for Neisseria lactamica (ATCC 23970). Clinical isolates from South Africa are depicted in ( c ) 29312 ( d ) 29306 ( e ) 41961 and ( f ) 41860. The scale bar represent 200 nm
    N Meningitidis Serogroup W Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    n meningitidis serogroup w strain - by Bioz Stars, 2020-04
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    ATCC n meningitidis b strain
    Transmission electron micrographs showing the presence of surface capsular polysaccharide for ( a ) Neisseria <t>meningitidis</t> <t>serogroup</t> W (ATCC 35559) and ( b ) absence of capsule for Neisseria lactamica (ATCC 23970). Clinical isolates from South Africa are depicted in ( c ) 29312 ( d ) 29306 ( e ) 41961 and ( f ) 41860. The scale bar represent 200 nm
    N Meningitidis B Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/n meningitidis b strain/product/ATCC
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    n meningitidis b strain - by Bioz Stars, 2020-04
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    Image Search Results


    Colorimetric and fluorescence based detection for NAATs in 3D printed reactor array. A) Representative photographs of colorimetric LAMP assay for detection of N. meningitidis with 0, 50, 500 and 5000 CFU/reaction on the same chip, alongside LAMP fluorescence based image at given time interval. B) LAMP amplification curves for P. falciparum with 0, 0.1 1, 10, 100, 1000 pg per reaction. C) Calibration curve for P. falciparum as function of log target concentration, n=3. D) LAMP amplification curves for N. meningitidis with 0, 50, 500, 5000 CFU per reaction. E) Calibration curve for N. meningitidis as function of log target concentration, n=3. WarmStart ® LAMP master mix was used.

    Journal: Biosensors & bioelectronics

    Article Title: Fully 3D Printed Integrated Reactor Array for Point-of-Care Molecular Diagnostics

    doi: 10.1016/j.bios.2018.03.009

    Figure Lengend Snippet: Colorimetric and fluorescence based detection for NAATs in 3D printed reactor array. A) Representative photographs of colorimetric LAMP assay for detection of N. meningitidis with 0, 50, 500 and 5000 CFU/reaction on the same chip, alongside LAMP fluorescence based image at given time interval. B) LAMP amplification curves for P. falciparum with 0, 0.1 1, 10, 100, 1000 pg per reaction. C) Calibration curve for P. falciparum as function of log target concentration, n=3. D) LAMP amplification curves for N. meningitidis with 0, 50, 500, 5000 CFU per reaction. E) Calibration curve for N. meningitidis as function of log target concentration, n=3. WarmStart ® LAMP master mix was used.

    Article Snippet: Bovine serum albumin (BSA), Poly (ethylene glycol) 8000 (PEG), poly(vinyl alcohol) (PVA) are from Sigma-Aldrich, RT-PCR grade water from Ambion, Inc., intercalating Eva Green fluorescent dye from Biotium, Isothermal Master Mix used in LAMP reaction buffer from ISO-001nd, OptiGene, Horsham, UK, WarmStart® colorimetric LAMP 2X master mix from New England Biolabs Inc. N. meningitidis (ATCC 13098) from American Type Culture Collection (ATCC, Rockville, MD).

    Techniques: Fluorescence, Lamp Assay, Chromatin Immunoprecipitation, Amplification, Concentration Assay

    Lack of cross-complementation of E. coli and N. meningitidis Skp. (A) The indicated N. meningitidis strains were grown in the presence or absence of 1 mM IPTG as specified below the gel and analyzed as cell lysates by denaturing SDS-PAGE and Coomassie

    Journal: Journal of Bacteriology

    Article Title: Role of the Periplasmic Chaperones Skp, SurA, and DegQ in Outer Membrane Protein Biogenesis in Neisseria meningitidis ▿ ▿ †

    doi: 10.1128/JB.00532-10

    Figure Lengend Snippet: Lack of cross-complementation of E. coli and N. meningitidis Skp. (A) The indicated N. meningitidis strains were grown in the presence or absence of 1 mM IPTG as specified below the gel and analyzed as cell lysates by denaturing SDS-PAGE and Coomassie

    Article Snippet: For liquid cultures, N. meningitidis was grown overnight on plates, from which it was swabbed into tryptic soy broth (TSB) (Becton Dickinson) to an optical density at 550 nm (OD550 ) of 0.1 and grown for 6 h with shaking.

    Techniques: SDS Page

    Immunofluorescence microscopy of WCs of strains MC58, MC58Lnt and MC58LntC. To confirm the presence of meningococcal cells, cells were incubated with FITC‐labelled rabbit polyclonal IgG raised against WC N. meningitidis (left and right panel). To compare FHbp cell surface expression between the strains, cells were also incubated with anti‐FHbp antibody JAR4 that was detected by Alexa Fluor 555 labelled donkey anti‐mouse IgG secondary antibody (right panel).

    Journal: British Journal of Pharmacology

    Article Title: The role of apolipoprotein N‐acyl transferase, Lnt, in the lipidation of factor H binding protein of Neisseria meningitidis strain MC58 and its potential as a drug target) The role of apolipoprotein N‐acyl transferase, Lnt, in the lipidation of factor H binding protein of Neisseria meningitidis strain MC58 and its potential as a drug target

    doi: 10.1111/bph.13660

    Figure Lengend Snippet: Immunofluorescence microscopy of WCs of strains MC58, MC58Lnt and MC58LntC. To confirm the presence of meningococcal cells, cells were incubated with FITC‐labelled rabbit polyclonal IgG raised against WC N. meningitidis (left and right panel). To compare FHbp cell surface expression between the strains, cells were also incubated with anti‐FHbp antibody JAR4 that was detected by Alexa Fluor 555 labelled donkey anti‐mouse IgG secondary antibody (right panel).

    Article Snippet: The wells were washed three times in PBS then PBS with 1% ( w / v ) BSA containing 1:500 dilution Alexa Fluor 555 labelled donkey anti‐mouse IgG secondary antibody (Abcam) was added (to fluorescently label the FHbp‐bound JAR4 antibody) and 1:500 dilution of FITC‐labelled rabbit polyclonal IgG raised against WC N. meningitidis (Abcam) to detect meningococcal cells.

    Techniques: Immunofluorescence, Microscopy, Incubation, Expressing

    Dynamic meningococcal disease progression selects for Opa expression. (A) Mice with mild-disease pattern were imaged over 4 days using a detection scale of 200/2000 counts. Bioluminescent signals from the mice were undetectable at 2 days (2d) post-infection. From day 3 (3d), the signals reappeared either in the brain (upper panel) or in the whole body (lower panel). Images are representative of each subgroup. Each panel follows one mouse over time. Bacterial loads in blood and CSF at indicated time points post-challenge are shown. (B) N. meningitidis was isolated from blood, CSF or nasal washes (N wash) at day 1, 3 and 4 after infection. Recovered isolates together with the initial inoculum, were analyzed for expression of Opa proteins (30 kDa), PilC (110 kDa), and pili (PilE subunit 16 kDa) by immunoblots. LOS (6.5 kDa) was analyzed by Tricine SDS-PAGE.

    Journal: PLoS ONE

    Article Title: Imaging of Disease Dynamics during Meningococcal Sepsis

    doi: 10.1371/journal.pone.0000241

    Figure Lengend Snippet: Dynamic meningococcal disease progression selects for Opa expression. (A) Mice with mild-disease pattern were imaged over 4 days using a detection scale of 200/2000 counts. Bioluminescent signals from the mice were undetectable at 2 days (2d) post-infection. From day 3 (3d), the signals reappeared either in the brain (upper panel) or in the whole body (lower panel). Images are representative of each subgroup. Each panel follows one mouse over time. Bacterial loads in blood and CSF at indicated time points post-challenge are shown. (B) N. meningitidis was isolated from blood, CSF or nasal washes (N wash) at day 1, 3 and 4 after infection. Recovered isolates together with the initial inoculum, were analyzed for expression of Opa proteins (30 kDa), PilC (110 kDa), and pili (PilE subunit 16 kDa) by immunoblots. LOS (6.5 kDa) was analyzed by Tricine SDS-PAGE.

    Article Snippet: For detection of meningococci the tissue sections were incubated with rabbit antibody against N. meningitidis (diluted 1∶50; USBiological) for 60 min, horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (diluted 1∶50; BioRad) for 60 min, and stained using ImmunoPure metal Enhanced DAB substrate (Pierce).

    Techniques: Expressing, Mouse Assay, Infection, Isolation, Western Blot, SDS Page

    Translocation of N. meningitidis to the respiratory mucosa is dependent on PilC1. (A) Bioluminescent imaging of mice and nasal washes spread on GC-plates 24 h post infection. CD46 transgenic mice were infected i.v. with wild-type FAM20 LU or the PilC1-deficient mutant FAM20Δ pilC1 LU . Nontransgenic mice were challenged with FAM20 LU . Bacterial counts in the nasal washes (CFU/ml) are presented. Significantly fewer PilC1 mutants were recovered compared with wild-type bacteria ( P

    Journal: PLoS ONE

    Article Title: Imaging of Disease Dynamics during Meningococcal Sepsis

    doi: 10.1371/journal.pone.0000241

    Figure Lengend Snippet: Translocation of N. meningitidis to the respiratory mucosa is dependent on PilC1. (A) Bioluminescent imaging of mice and nasal washes spread on GC-plates 24 h post infection. CD46 transgenic mice were infected i.v. with wild-type FAM20 LU or the PilC1-deficient mutant FAM20Δ pilC1 LU . Nontransgenic mice were challenged with FAM20 LU . Bacterial counts in the nasal washes (CFU/ml) are presented. Significantly fewer PilC1 mutants were recovered compared with wild-type bacteria ( P

    Article Snippet: For detection of meningococci the tissue sections were incubated with rabbit antibody against N. meningitidis (diluted 1∶50; USBiological) for 60 min, horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (diluted 1∶50; BioRad) for 60 min, and stained using ImmunoPure metal Enhanced DAB substrate (Pierce).

    Techniques: Translocation Assay, Imaging, Mouse Assay, Infection, Transgenic Assay, Mutagenesis

    N. meningitidis targets the thyroid gland and the nasal area of CD46 transgenic mice. (A) BLI of mice at 24 h after i.v. infection. Distinct bioluminescent signals are visible in the thyroid area (T) and oral-nasal region (N) of CD46 transgenic mice. (B) Ex vivo BLI imaging of a dissected thyroid. (C) Bacterial loads in blood and thyroid at 24 h post-infection. Significant difference between thyroid of CD46 transgenic mice and nontransgenic mice is indicated ( P

    Journal: PLoS ONE

    Article Title: Imaging of Disease Dynamics during Meningococcal Sepsis

    doi: 10.1371/journal.pone.0000241

    Figure Lengend Snippet: N. meningitidis targets the thyroid gland and the nasal area of CD46 transgenic mice. (A) BLI of mice at 24 h after i.v. infection. Distinct bioluminescent signals are visible in the thyroid area (T) and oral-nasal region (N) of CD46 transgenic mice. (B) Ex vivo BLI imaging of a dissected thyroid. (C) Bacterial loads in blood and thyroid at 24 h post-infection. Significant difference between thyroid of CD46 transgenic mice and nontransgenic mice is indicated ( P

    Article Snippet: For detection of meningococci the tissue sections were incubated with rabbit antibody against N. meningitidis (diluted 1∶50; USBiological) for 60 min, horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (diluted 1∶50; BioRad) for 60 min, and stained using ImmunoPure metal Enhanced DAB substrate (Pierce).

    Techniques: Transgenic Assay, Mouse Assay, Infection, Ex Vivo, Imaging

    Generation and characterization of the bioluminescent N. meningitidis strain FAM20 LU . (A) Chromosomal integration of a bioluminescence expression cassette into strain FAM20. The luxCDABE operon of plasmid pLKMp is flanked by the Neisseria specific porA promoter (P) and terminator (T) sequences of the gapdh gene. The kanamycin resistance gene, kanR , enables selection on agar-plates. UHS and DHS represent two sequences homologous to a non-coding region between orf73 and orf74 of the FAM20 genome. (B) The porA promoter enhances bioluminescent emission. Bioluminescence (in photon units) emitted from N. meningitidis strain FAM20 transformed with pLKMp (black line) or transformed with control vector without promoter (grey line) is plotted against bacterial counts. (C) In vivo BLI of mice challenged i.p. with 10 8 FAM20 LU . Mice were imaged at indicated time points post-infection. In contrast to nontransgenic mice (upper panel), strong signals in the body together with focal signals in the brain were detected in CD46 transgenic mice (lower panel). Images show one representative mouse from each group.

    Journal: PLoS ONE

    Article Title: Imaging of Disease Dynamics during Meningococcal Sepsis

    doi: 10.1371/journal.pone.0000241

    Figure Lengend Snippet: Generation and characterization of the bioluminescent N. meningitidis strain FAM20 LU . (A) Chromosomal integration of a bioluminescence expression cassette into strain FAM20. The luxCDABE operon of plasmid pLKMp is flanked by the Neisseria specific porA promoter (P) and terminator (T) sequences of the gapdh gene. The kanamycin resistance gene, kanR , enables selection on agar-plates. UHS and DHS represent two sequences homologous to a non-coding region between orf73 and orf74 of the FAM20 genome. (B) The porA promoter enhances bioluminescent emission. Bioluminescence (in photon units) emitted from N. meningitidis strain FAM20 transformed with pLKMp (black line) or transformed with control vector without promoter (grey line) is plotted against bacterial counts. (C) In vivo BLI of mice challenged i.p. with 10 8 FAM20 LU . Mice were imaged at indicated time points post-infection. In contrast to nontransgenic mice (upper panel), strong signals in the body together with focal signals in the brain were detected in CD46 transgenic mice (lower panel). Images show one representative mouse from each group.

    Article Snippet: For detection of meningococci the tissue sections were incubated with rabbit antibody against N. meningitidis (diluted 1∶50; USBiological) for 60 min, horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (diluted 1∶50; BioRad) for 60 min, and stained using ImmunoPure metal Enhanced DAB substrate (Pierce).

    Techniques: Expressing, Plasmid Preparation, Selection, Transformation Assay, In Vivo, Mouse Assay, Infection, Transgenic Assay

    Bactericidal activity of protein A–purified mAb Me-1 against N. meningitidis strain 608B. Guinea pig serum ( closed circles ) or heat inactivated guinea pig serum ( open circles ) were used as the source of complement to evaluate the bactericidal activity.

    Journal: The Journal of Experimental Medicine

    Article Title: Highly Conserved Neisseria meningitidis Surface Protein Confers Protection against Experimental Infection

    doi:

    Figure Lengend Snippet: Bactericidal activity of protein A–purified mAb Me-1 against N. meningitidis strain 608B. Guinea pig serum ( closed circles ) or heat inactivated guinea pig serum ( open circles ) were used as the source of complement to evaluate the bactericidal activity.

    Article Snippet: A λGEM-11 genomic DNA library from N. meningitidis strain 608B was constructed according to the manufacturer's recommendations ( Promega , Madison, WI).

    Techniques: Activity Assay, Purification

    Nucleotide and amino acid sequences of the gene encoding the N. meningitidis strain 608B NspA protein. The open reading frame of 525 bp extends from the start codon at base 143 to the stop codon at base 667. A 19–amino acid leader peptide is underlined, and a putative ribosome binding site is boxed. These sequence data are available from GenBank under accession number U52066.

    Journal: The Journal of Experimental Medicine

    Article Title: Highly Conserved Neisseria meningitidis Surface Protein Confers Protection against Experimental Infection

    doi:

    Figure Lengend Snippet: Nucleotide and amino acid sequences of the gene encoding the N. meningitidis strain 608B NspA protein. The open reading frame of 525 bp extends from the start codon at base 143 to the stop codon at base 667. A 19–amino acid leader peptide is underlined, and a putative ribosome binding site is boxed. These sequence data are available from GenBank under accession number U52066.

    Article Snippet: A λGEM-11 genomic DNA library from N. meningitidis strain 608B was constructed according to the manufacturer's recommendations ( Promega , Madison, WI).

    Techniques: Binding Assay, Sequencing

    N. meningitidis do not cross the epithelial layer. (A) Z-stack from confocal 3D reconstruction of Calu-3 cell layer infected 24 h with 10 6 bacteria of the wild-type strain (2C4.3 WT) or the strain defective for type IV pili (2C4.3 Δ pilE ). The Calu-3 cell layer was fixed and immunostained with anti-2C4.3 antibody (green). Cells were stained with Alexa Fluor-conjugated phalloidin (red), and nuclei were stained with DAPI (blue). Bar, 20 μm. (B) Transmission electron microscopy images (longitudinal sections) of Calu-3 cell layer infected for 24 h with 10 6 wild-type meningococci. (C) Kaplan-Meier plots showing the traversal of wild-type N. meningitidis across the Calu-3 cell layer. Data were expressed as percentages of invaded basal chamber. For untreated cells, eight filters were infected with 10 4 or 10 2 bacteria, and three independent experiments were performed. For IL-4- or IL-13-treated cells, four filters were infected with 10 4 or 10 2 bacteria, and two independent experiments were performed.

    Journal: mSphere

    Article Title: Airway Mucus Restricts Neisseria meningitidis Away from Nasopharyngeal Epithelial Cells and Protects the Mucosa from Inflammation

    doi: 10.1128/mSphere.00494-19

    Figure Lengend Snippet: N. meningitidis do not cross the epithelial layer. (A) Z-stack from confocal 3D reconstruction of Calu-3 cell layer infected 24 h with 10 6 bacteria of the wild-type strain (2C4.3 WT) or the strain defective for type IV pili (2C4.3 Δ pilE ). The Calu-3 cell layer was fixed and immunostained with anti-2C4.3 antibody (green). Cells were stained with Alexa Fluor-conjugated phalloidin (red), and nuclei were stained with DAPI (blue). Bar, 20 μm. (B) Transmission electron microscopy images (longitudinal sections) of Calu-3 cell layer infected for 24 h with 10 6 wild-type meningococci. (C) Kaplan-Meier plots showing the traversal of wild-type N. meningitidis across the Calu-3 cell layer. Data were expressed as percentages of invaded basal chamber. For untreated cells, eight filters were infected with 10 4 or 10 2 bacteria, and three independent experiments were performed. For IL-4- or IL-13-treated cells, four filters were infected with 10 4 or 10 2 bacteria, and two independent experiments were performed.

    Article Snippet: The cells were then incubated with an anti- N. meningitidis strain 2C4.3 (anti-2C4.3) (and an anti-MUC5AC monoclonal antibody (clone 45M1; Life Technologies) in staining buffer for 2 h. After three washes in PBS, the filters were incubated with Alexa Fluor-conjugated secondary antibodies for 2 h. Nuclear DNA and actin were stained with 4′,6′-diamidino-2-phenylindole (DAPI) at 1 μg/ml and Alexa Fluor-conjugated phalloidin (Invitrogen), respectively.

    Techniques: Infection, Staining, Transmission Assay, Electron Microscopy

    N. meningitidis colonizes the outer mucus. Calu-3 cells grown in AIC were infected for 24 h with 10 6 bacteria. (A) After Calu-3 cells were infected with wild-type (WT) or Δ pilE mutant of N. meningitidis strain 2C4.3, the outer layer of the mucus (outer mucus) was dissociated from the cell-attached mucus using N -acetylcysteine. Bacterial load in the N -acetylcysteine fraction or the cell-attached fraction was determined. Data were expressed as mean percentages of CFU ± SEM for at least five filters from three independent experiments. (B) Scanning electron microscopy images showing bacteria trapped in the mucus. Bacteria trapped in mucus (red asterisk) and bacteria that directly interact with Calu-3 cells (red arrow) are indicated. Bars, 10 μm. (C) Transmission electron microscopy images showing bacteria in the mucus (left) or in contact with cells (right). A dying bacterium (red asterisk) and bacteria adherent to Calu-3 cells (red arrows) are indicated. (D) Z-stack from confocal 3D reconstruction of two different Calu-3 cell layers infected with N. meningitidis . Calu-3 cell layers were fixed and immunostained with anti-2C4.3 antibody (green) and anti-MUC5AC antibody (purple). Cells were stained with A546-phalloidin (red), and nuclei were stained with DAPI (blue). Bar, 20 μm.

    Journal: mSphere

    Article Title: Airway Mucus Restricts Neisseria meningitidis Away from Nasopharyngeal Epithelial Cells and Protects the Mucosa from Inflammation

    doi: 10.1128/mSphere.00494-19

    Figure Lengend Snippet: N. meningitidis colonizes the outer mucus. Calu-3 cells grown in AIC were infected for 24 h with 10 6 bacteria. (A) After Calu-3 cells were infected with wild-type (WT) or Δ pilE mutant of N. meningitidis strain 2C4.3, the outer layer of the mucus (outer mucus) was dissociated from the cell-attached mucus using N -acetylcysteine. Bacterial load in the N -acetylcysteine fraction or the cell-attached fraction was determined. Data were expressed as mean percentages of CFU ± SEM for at least five filters from three independent experiments. (B) Scanning electron microscopy images showing bacteria trapped in the mucus. Bacteria trapped in mucus (red asterisk) and bacteria that directly interact with Calu-3 cells (red arrow) are indicated. Bars, 10 μm. (C) Transmission electron microscopy images showing bacteria in the mucus (left) or in contact with cells (right). A dying bacterium (red asterisk) and bacteria adherent to Calu-3 cells (red arrows) are indicated. (D) Z-stack from confocal 3D reconstruction of two different Calu-3 cell layers infected with N. meningitidis . Calu-3 cell layers were fixed and immunostained with anti-2C4.3 antibody (green) and anti-MUC5AC antibody (purple). Cells were stained with A546-phalloidin (red), and nuclei were stained with DAPI (blue). Bar, 20 μm.

    Article Snippet: The cells were then incubated with an anti- N. meningitidis strain 2C4.3 (anti-2C4.3) (and an anti-MUC5AC monoclonal antibody (clone 45M1; Life Technologies) in staining buffer for 2 h. After three washes in PBS, the filters were incubated with Alexa Fluor-conjugated secondary antibodies for 2 h. Nuclear DNA and actin were stained with 4′,6′-diamidino-2-phenylindole (DAPI) at 1 μg/ml and Alexa Fluor-conjugated phalloidin (Invitrogen), respectively.

    Techniques: Infection, Mutagenesis, Electron Microscopy, Transmission Assay, Staining

    Transmission electron micrographs showing the presence of surface capsular polysaccharide for ( a ) Neisseria meningitidis serogroup W (ATCC 35559) and ( b ) absence of capsule for Neisseria lactamica (ATCC 23970). Clinical isolates from South Africa are depicted in ( c ) 29312 ( d ) 29306 ( e ) 41961 and ( f ) 41860. The scale bar represent 200 nm

    Journal: BMC Microbiology

    Article Title: Molecular characterization of invasive capsule null Neisseria meningitidis in South Africa

    doi: 10.1186/s12866-017-0942-5

    Figure Lengend Snippet: Transmission electron micrographs showing the presence of surface capsular polysaccharide for ( a ) Neisseria meningitidis serogroup W (ATCC 35559) and ( b ) absence of capsule for Neisseria lactamica (ATCC 23970). Clinical isolates from South Africa are depicted in ( c ) 29312 ( d ) 29306 ( e ) 41961 and ( f ) 41860. The scale bar represent 200 nm

    Article Snippet: American Type Culture Collection (ATCC®) isolate M-603, a N. meningitidis serogroup W strain (ATCC-35559™) was used as an encapsulated control.

    Techniques: Transmission Assay