n-terminal his-tag Search Results


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  • 92
    Thermo Fisher alicator lic cloning
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    Preparation and characterization of <t>PrA-ssDNA</t> tags. ( a ) Scheme of PrA-ssDNA conjugation procedure. Amine-functionalized ssDNA is activated by sulfo-SMCC, while <t>HIS-tagged</t> cysteine-terminated PrA (supplied in an oxidized PrA-S-S-PrA form) is reduced by TCEP. Activated ssDNA and reduced PrA are reacted to form covalent PrA-ssDNA bioconjugates and purified from the excess ssDNA with HIS SpinTrap column. ( b ) Verification of PrA-ssDNA conjugation and purity with SDS-PAGE: (1) Reference protein ladder (KDa); (2) PrA-S-S-PrA dimer; (3) PrA-SH reduced by TCEP; (4) PrA-ssDNA bioconjugate. ( c,d ) Functionality of PrA-ssDNA tags was assessed by staining of model targets (Lamin A, HSP90, and Cox4) in fixed HeLa cells with pre-assembled 1′Ab/PrA-ssDNA probes followed by complementary ssDNA′-AP ( c ) in comparison to conventional 2-step immunostaining with unmodified 1′Ab followed by 2′Ab-AP probes ( d ). Scale bar, 50 μm.
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    Thermo Fisher n terminal his tag
    Preparation and characterization of <t>PrA-ssDNA</t> tags. ( a ) Scheme of PrA-ssDNA conjugation procedure. Amine-functionalized ssDNA is activated by sulfo-SMCC, while <t>HIS-tagged</t> cysteine-terminated PrA (supplied in an oxidized PrA-S-S-PrA form) is reduced by TCEP. Activated ssDNA and reduced PrA are reacted to form covalent PrA-ssDNA bioconjugates and purified from the excess ssDNA with HIS SpinTrap column. ( b ) Verification of PrA-ssDNA conjugation and purity with SDS-PAGE: (1) Reference protein ladder (KDa); (2) PrA-S-S-PrA dimer; (3) PrA-SH reduced by TCEP; (4) PrA-ssDNA bioconjugate. ( c,d ) Functionality of PrA-ssDNA tags was assessed by staining of model targets (Lamin A, HSP90, and Cox4) in fixed HeLa cells with pre-assembled 1′Ab/PrA-ssDNA probes followed by complementary ssDNA′-AP ( c ) in comparison to conventional 2-step immunostaining with unmodified 1′Ab followed by 2′Ab-AP probes ( d ). Scale bar, 50 μm.
    N Terminal His Tag, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 540 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare n terminal his tag
    Preparation and characterization of <t>PrA-ssDNA</t> tags. ( a ) Scheme of PrA-ssDNA conjugation procedure. Amine-functionalized ssDNA is activated by sulfo-SMCC, while <t>HIS-tagged</t> cysteine-terminated PrA (supplied in an oxidized PrA-S-S-PrA form) is reduced by TCEP. Activated ssDNA and reduced PrA are reacted to form covalent PrA-ssDNA bioconjugates and purified from the excess ssDNA with HIS SpinTrap column. ( b ) Verification of PrA-ssDNA conjugation and purity with SDS-PAGE: (1) Reference protein ladder (KDa); (2) PrA-S-S-PrA dimer; (3) PrA-SH reduced by TCEP; (4) PrA-ssDNA bioconjugate. ( c,d ) Functionality of PrA-ssDNA tags was assessed by staining of model targets (Lamin A, HSP90, and Cox4) in fixed HeLa cells with pre-assembled 1′Ab/PrA-ssDNA probes followed by complementary ssDNA′-AP ( c ) in comparison to conventional 2-step immunostaining with unmodified 1′Ab followed by 2′Ab-AP probes ( d ). Scale bar, 50 μm.
    N Terminal His Tag, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 395 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Genewiz n terminal his tag
    Preparation and characterization of <t>PrA-ssDNA</t> tags. ( a ) Scheme of PrA-ssDNA conjugation procedure. Amine-functionalized ssDNA is activated by sulfo-SMCC, while <t>HIS-tagged</t> cysteine-terminated PrA (supplied in an oxidized PrA-S-S-PrA form) is reduced by TCEP. Activated ssDNA and reduced PrA are reacted to form covalent PrA-ssDNA bioconjugates and purified from the excess ssDNA with HIS SpinTrap column. ( b ) Verification of PrA-ssDNA conjugation and purity with SDS-PAGE: (1) Reference protein ladder (KDa); (2) PrA-S-S-PrA dimer; (3) PrA-SH reduced by TCEP; (4) PrA-ssDNA bioconjugate. ( c,d ) Functionality of PrA-ssDNA tags was assessed by staining of model targets (Lamin A, HSP90, and Cox4) in fixed HeLa cells with pre-assembled 1′Ab/PrA-ssDNA probes followed by complementary ssDNA′-AP ( c ) in comparison to conventional 2-step immunostaining with unmodified 1′Ab followed by 2′Ab-AP probes ( d ). Scale bar, 50 μm.
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    Syntaxin n terminal his tag
    Preparation and characterization of <t>PrA-ssDNA</t> tags. ( a ) Scheme of PrA-ssDNA conjugation procedure. Amine-functionalized ssDNA is activated by sulfo-SMCC, while <t>HIS-tagged</t> cysteine-terminated PrA (supplied in an oxidized PrA-S-S-PrA form) is reduced by TCEP. Activated ssDNA and reduced PrA are reacted to form covalent PrA-ssDNA bioconjugates and purified from the excess ssDNA with HIS SpinTrap column. ( b ) Verification of PrA-ssDNA conjugation and purity with SDS-PAGE: (1) Reference protein ladder (KDa); (2) PrA-S-S-PrA dimer; (3) PrA-SH reduced by TCEP; (4) PrA-ssDNA bioconjugate. ( c,d ) Functionality of PrA-ssDNA tags was assessed by staining of model targets (Lamin A, HSP90, and Cox4) in fixed HeLa cells with pre-assembled 1′Ab/PrA-ssDNA probes followed by complementary ssDNA′-AP ( c ) in comparison to conventional 2-step immunostaining with unmodified 1′Ab followed by 2′Ab-AP probes ( d ). Scale bar, 50 μm.
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    Affibody n terminal his tag
    Preparation and characterization of <t>PrA-ssDNA</t> tags. ( a ) Scheme of PrA-ssDNA conjugation procedure. Amine-functionalized ssDNA is activated by sulfo-SMCC, while <t>HIS-tagged</t> cysteine-terminated PrA (supplied in an oxidized PrA-S-S-PrA form) is reduced by TCEP. Activated ssDNA and reduced PrA are reacted to form covalent PrA-ssDNA bioconjugates and purified from the excess ssDNA with HIS SpinTrap column. ( b ) Verification of PrA-ssDNA conjugation and purity with SDS-PAGE: (1) Reference protein ladder (KDa); (2) PrA-S-S-PrA dimer; (3) PrA-SH reduced by TCEP; (4) PrA-ssDNA bioconjugate. ( c,d ) Functionality of PrA-ssDNA tags was assessed by staining of model targets (Lamin A, HSP90, and Cox4) in fixed HeLa cells with pre-assembled 1′Ab/PrA-ssDNA probes followed by complementary ssDNA′-AP ( c ) in comparison to conventional 2-step immunostaining with unmodified 1′Ab followed by 2′Ab-AP probes ( d ). Scale bar, 50 μm.
    N Terminal His Tag, supplied by Affibody, used in various techniques. Bioz Stars score: 91/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen n terminal his tag
    Preparation and characterization of <t>PrA-ssDNA</t> tags. ( a ) Scheme of PrA-ssDNA conjugation procedure. Amine-functionalized ssDNA is activated by sulfo-SMCC, while <t>HIS-tagged</t> cysteine-terminated PrA (supplied in an oxidized PrA-S-S-PrA form) is reduced by TCEP. Activated ssDNA and reduced PrA are reacted to form covalent PrA-ssDNA bioconjugates and purified from the excess ssDNA with HIS SpinTrap column. ( b ) Verification of PrA-ssDNA conjugation and purity with SDS-PAGE: (1) Reference protein ladder (KDa); (2) PrA-S-S-PrA dimer; (3) PrA-SH reduced by TCEP; (4) PrA-ssDNA bioconjugate. ( c,d ) Functionality of PrA-ssDNA tags was assessed by staining of model targets (Lamin A, HSP90, and Cox4) in fixed HeLa cells with pre-assembled 1′Ab/PrA-ssDNA probes followed by complementary ssDNA′-AP ( c ) in comparison to conventional 2-step immunostaining with unmodified 1′Ab followed by 2′Ab-AP probes ( d ). Scale bar, 50 μm.
    N Terminal His Tag, supplied by Qiagen, used in various techniques. Bioz Stars score: 92/100, based on 295 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Addgene inc noncleavable n terminal his tag
    Preparation and characterization of <t>PrA-ssDNA</t> tags. ( a ) Scheme of PrA-ssDNA conjugation procedure. Amine-functionalized ssDNA is activated by sulfo-SMCC, while <t>HIS-tagged</t> cysteine-terminated PrA (supplied in an oxidized PrA-S-S-PrA form) is reduced by TCEP. Activated ssDNA and reduced PrA are reacted to form covalent PrA-ssDNA bioconjugates and purified from the excess ssDNA with HIS SpinTrap column. ( b ) Verification of PrA-ssDNA conjugation and purity with SDS-PAGE: (1) Reference protein ladder (KDa); (2) PrA-S-S-PrA dimer; (3) PrA-SH reduced by TCEP; (4) PrA-ssDNA bioconjugate. ( c,d ) Functionality of PrA-ssDNA tags was assessed by staining of model targets (Lamin A, HSP90, and Cox4) in fixed HeLa cells with pre-assembled 1′Ab/PrA-ssDNA probes followed by complementary ssDNA′-AP ( c ) in comparison to conventional 2-step immunostaining with unmodified 1′Ab followed by 2′Ab-AP probes ( d ). Scale bar, 50 μm.
    Noncleavable N Terminal His Tag, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher n terminal his tag vector prset a
    Preparation and characterization of <t>PrA-ssDNA</t> tags. ( a ) Scheme of PrA-ssDNA conjugation procedure. Amine-functionalized ssDNA is activated by sulfo-SMCC, while <t>HIS-tagged</t> cysteine-terminated PrA (supplied in an oxidized PrA-S-S-PrA form) is reduced by TCEP. Activated ssDNA and reduced PrA are reacted to form covalent PrA-ssDNA bioconjugates and purified from the excess ssDNA with HIS SpinTrap column. ( b ) Verification of PrA-ssDNA conjugation and purity with SDS-PAGE: (1) Reference protein ladder (KDa); (2) PrA-S-S-PrA dimer; (3) PrA-SH reduced by TCEP; (4) PrA-ssDNA bioconjugate. ( c,d ) Functionality of PrA-ssDNA tags was assessed by staining of model targets (Lamin A, HSP90, and Cox4) in fixed HeLa cells with pre-assembled 1′Ab/PrA-ssDNA probes followed by complementary ssDNA′-AP ( c ) in comparison to conventional 2-step immunostaining with unmodified 1′Ab followed by 2′Ab-AP probes ( d ). Scale bar, 50 μm.
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    Seca Inc n terminal his tag substituted seca
    Preparation and characterization of <t>PrA-ssDNA</t> tags. ( a ) Scheme of PrA-ssDNA conjugation procedure. Amine-functionalized ssDNA is activated by sulfo-SMCC, while <t>HIS-tagged</t> cysteine-terminated PrA (supplied in an oxidized PrA-S-S-PrA form) is reduced by TCEP. Activated ssDNA and reduced PrA are reacted to form covalent PrA-ssDNA bioconjugates and purified from the excess ssDNA with HIS SpinTrap column. ( b ) Verification of PrA-ssDNA conjugation and purity with SDS-PAGE: (1) Reference protein ladder (KDa); (2) PrA-S-S-PrA dimer; (3) PrA-SH reduced by TCEP; (4) PrA-ssDNA bioconjugate. ( c,d ) Functionality of PrA-ssDNA tags was assessed by staining of model targets (Lamin A, HSP90, and Cox4) in fixed HeLa cells with pre-assembled 1′Ab/PrA-ssDNA probes followed by complementary ssDNA′-AP ( c ) in comparison to conventional 2-step immunostaining with unmodified 1′Ab followed by 2′Ab-AP probes ( d ). Scale bar, 50 μm.
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    Preparation and characterization of <t>PrA-ssDNA</t> tags. ( a ) Scheme of PrA-ssDNA conjugation procedure. Amine-functionalized ssDNA is activated by sulfo-SMCC, while <t>HIS-tagged</t> cysteine-terminated PrA (supplied in an oxidized PrA-S-S-PrA form) is reduced by TCEP. Activated ssDNA and reduced PrA are reacted to form covalent PrA-ssDNA bioconjugates and purified from the excess ssDNA with HIS SpinTrap column. ( b ) Verification of PrA-ssDNA conjugation and purity with SDS-PAGE: (1) Reference protein ladder (KDa); (2) PrA-S-S-PrA dimer; (3) PrA-SH reduced by TCEP; (4) PrA-ssDNA bioconjugate. ( c,d ) Functionality of PrA-ssDNA tags was assessed by staining of model targets (Lamin A, HSP90, and Cox4) in fixed HeLa cells with pre-assembled 1′Ab/PrA-ssDNA probes followed by complementary ssDNA′-AP ( c ) in comparison to conventional 2-step immunostaining with unmodified 1′Ab followed by 2′Ab-AP probes ( d ). Scale bar, 50 μm.
    Thrombin Cleavable N Terminal His Tag, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher tev protease cleavable n terminal his tag
    Preparation and characterization of <t>PrA-ssDNA</t> tags. ( a ) Scheme of PrA-ssDNA conjugation procedure. Amine-functionalized ssDNA is activated by sulfo-SMCC, while <t>HIS-tagged</t> cysteine-terminated PrA (supplied in an oxidized PrA-S-S-PrA form) is reduced by TCEP. Activated ssDNA and reduced PrA are reacted to form covalent PrA-ssDNA bioconjugates and purified from the excess ssDNA with HIS SpinTrap column. ( b ) Verification of PrA-ssDNA conjugation and purity with SDS-PAGE: (1) Reference protein ladder (KDa); (2) PrA-S-S-PrA dimer; (3) PrA-SH reduced by TCEP; (4) PrA-ssDNA bioconjugate. ( c,d ) Functionality of PrA-ssDNA tags was assessed by staining of model targets (Lamin A, HSP90, and Cox4) in fixed HeLa cells with pre-assembled 1′Ab/PrA-ssDNA probes followed by complementary ssDNA′-AP ( c ) in comparison to conventional 2-step immunostaining with unmodified 1′Ab followed by 2′Ab-AP probes ( d ). Scale bar, 50 μm.
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    Thermo Fisher n terminal his tag encoding expression plasmid pdet17
    Preparation and characterization of <t>PrA-ssDNA</t> tags. ( a ) Scheme of PrA-ssDNA conjugation procedure. Amine-functionalized ssDNA is activated by sulfo-SMCC, while <t>HIS-tagged</t> cysteine-terminated PrA (supplied in an oxidized PrA-S-S-PrA form) is reduced by TCEP. Activated ssDNA and reduced PrA are reacted to form covalent PrA-ssDNA bioconjugates and purified from the excess ssDNA with HIS SpinTrap column. ( b ) Verification of PrA-ssDNA conjugation and purity with SDS-PAGE: (1) Reference protein ladder (KDa); (2) PrA-S-S-PrA dimer; (3) PrA-SH reduced by TCEP; (4) PrA-ssDNA bioconjugate. ( c,d ) Functionality of PrA-ssDNA tags was assessed by staining of model targets (Lamin A, HSP90, and Cox4) in fixed HeLa cells with pre-assembled 1′Ab/PrA-ssDNA probes followed by complementary ssDNA′-AP ( c ) in comparison to conventional 2-step immunostaining with unmodified 1′Ab followed by 2′Ab-AP probes ( d ). Scale bar, 50 μm.
    N Terminal His Tag Encoding Expression Plasmid Pdet17, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen n terminal his tag expression vector pqe30
    Preparation and characterization of <t>PrA-ssDNA</t> tags. ( a ) Scheme of PrA-ssDNA conjugation procedure. Amine-functionalized ssDNA is activated by sulfo-SMCC, while <t>HIS-tagged</t> cysteine-terminated PrA (supplied in an oxidized PrA-S-S-PrA form) is reduced by TCEP. Activated ssDNA and reduced PrA are reacted to form covalent PrA-ssDNA bioconjugates and purified from the excess ssDNA with HIS SpinTrap column. ( b ) Verification of PrA-ssDNA conjugation and purity with SDS-PAGE: (1) Reference protein ladder (KDa); (2) PrA-S-S-PrA dimer; (3) PrA-SH reduced by TCEP; (4) PrA-ssDNA bioconjugate. ( c,d ) Functionality of PrA-ssDNA tags was assessed by staining of model targets (Lamin A, HSP90, and Cox4) in fixed HeLa cells with pre-assembled 1′Ab/PrA-ssDNA probes followed by complementary ssDNA′-AP ( c ) in comparison to conventional 2-step immunostaining with unmodified 1′Ab followed by 2′Ab-AP probes ( d ). Scale bar, 50 μm.
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    GenScript tev cleavable poly his
    Preparation and characterization of <t>PrA-ssDNA</t> tags. ( a ) Scheme of PrA-ssDNA conjugation procedure. Amine-functionalized ssDNA is activated by sulfo-SMCC, while <t>HIS-tagged</t> cysteine-terminated PrA (supplied in an oxidized PrA-S-S-PrA form) is reduced by TCEP. Activated ssDNA and reduced PrA are reacted to form covalent PrA-ssDNA bioconjugates and purified from the excess ssDNA with HIS SpinTrap column. ( b ) Verification of PrA-ssDNA conjugation and purity with SDS-PAGE: (1) Reference protein ladder (KDa); (2) PrA-S-S-PrA dimer; (3) PrA-SH reduced by TCEP; (4) PrA-ssDNA bioconjugate. ( c,d ) Functionality of PrA-ssDNA tags was assessed by staining of model targets (Lamin A, HSP90, and Cox4) in fixed HeLa cells with pre-assembled 1′Ab/PrA-ssDNA probes followed by complementary ssDNA′-AP ( c ) in comparison to conventional 2-step immunostaining with unmodified 1′Ab followed by 2′Ab-AP probes ( d ). Scale bar, 50 μm.
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    Millipore his tagged hrv 3c protease
    Preparation and characterization of <t>PrA-ssDNA</t> tags. ( a ) Scheme of PrA-ssDNA conjugation procedure. Amine-functionalized ssDNA is activated by sulfo-SMCC, while <t>HIS-tagged</t> cysteine-terminated PrA (supplied in an oxidized PrA-S-S-PrA form) is reduced by TCEP. Activated ssDNA and reduced PrA are reacted to form covalent PrA-ssDNA bioconjugates and purified from the excess ssDNA with HIS SpinTrap column. ( b ) Verification of PrA-ssDNA conjugation and purity with SDS-PAGE: (1) Reference protein ladder (KDa); (2) PrA-S-S-PrA dimer; (3) PrA-SH reduced by TCEP; (4) PrA-ssDNA bioconjugate. ( c,d ) Functionality of PrA-ssDNA tags was assessed by staining of model targets (Lamin A, HSP90, and Cox4) in fixed HeLa cells with pre-assembled 1′Ab/PrA-ssDNA probes followed by complementary ssDNA′-AP ( c ) in comparison to conventional 2-step immunostaining with unmodified 1′Ab followed by 2′Ab-AP probes ( d ). Scale bar, 50 μm.
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    Image Search Results


    Preparation and characterization of PrA-ssDNA tags. ( a ) Scheme of PrA-ssDNA conjugation procedure. Amine-functionalized ssDNA is activated by sulfo-SMCC, while HIS-tagged cysteine-terminated PrA (supplied in an oxidized PrA-S-S-PrA form) is reduced by TCEP. Activated ssDNA and reduced PrA are reacted to form covalent PrA-ssDNA bioconjugates and purified from the excess ssDNA with HIS SpinTrap column. ( b ) Verification of PrA-ssDNA conjugation and purity with SDS-PAGE: (1) Reference protein ladder (KDa); (2) PrA-S-S-PrA dimer; (3) PrA-SH reduced by TCEP; (4) PrA-ssDNA bioconjugate. ( c,d ) Functionality of PrA-ssDNA tags was assessed by staining of model targets (Lamin A, HSP90, and Cox4) in fixed HeLa cells with pre-assembled 1′Ab/PrA-ssDNA probes followed by complementary ssDNA′-AP ( c ) in comparison to conventional 2-step immunostaining with unmodified 1′Ab followed by 2′Ab-AP probes ( d ). Scale bar, 50 μm.

    Journal: Scientific Reports

    Article Title: Multiplexed In-cell Immunoassay for Same-sample Protein Expression Profiling

    doi: 10.1038/srep13651

    Figure Lengend Snippet: Preparation and characterization of PrA-ssDNA tags. ( a ) Scheme of PrA-ssDNA conjugation procedure. Amine-functionalized ssDNA is activated by sulfo-SMCC, while HIS-tagged cysteine-terminated PrA (supplied in an oxidized PrA-S-S-PrA form) is reduced by TCEP. Activated ssDNA and reduced PrA are reacted to form covalent PrA-ssDNA bioconjugates and purified from the excess ssDNA with HIS SpinTrap column. ( b ) Verification of PrA-ssDNA conjugation and purity with SDS-PAGE: (1) Reference protein ladder (KDa); (2) PrA-S-S-PrA dimer; (3) PrA-SH reduced by TCEP; (4) PrA-ssDNA bioconjugate. ( c,d ) Functionality of PrA-ssDNA tags was assessed by staining of model targets (Lamin A, HSP90, and Cox4) in fixed HeLa cells with pre-assembled 1′Ab/PrA-ssDNA probes followed by complementary ssDNA′-AP ( c ) in comparison to conventional 2-step immunostaining with unmodified 1′Ab followed by 2′Ab-AP probes ( d ). Scale bar, 50 μm.

    Article Snippet: Materials Protein A with C-terminal cysteine and N-terminal HIS-tag (PrA-SH) was purchased from Abcam.

    Techniques: Conjugation Assay, Purification, SDS Page, Staining, Immunostaining